CA3092916A1 - Thermolabile proteinases - Google Patents

Thermolabile proteinases Download PDF

Info

Publication number
CA3092916A1
CA3092916A1 CA3092916A CA3092916A CA3092916A1 CA 3092916 A1 CA3092916 A1 CA 3092916A1 CA 3092916 A CA3092916 A CA 3092916A CA 3092916 A CA3092916 A CA 3092916A CA 3092916 A1 CA3092916 A1 CA 3092916A1
Authority
CA
Canada
Prior art keywords
sample
proteinase
concentration
minutes
heating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3092916A
Other languages
English (en)
French (fr)
Inventor
Bernd Ketelsen STRIBERNY
Cathrine PEDERSEN
Jorn Remi Henriksen
Olav Lanes
Marit Sjo Lorentzen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Arcticzymes ASA
Original Assignee
Arcticzymes ASA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arcticzymes ASA filed Critical Arcticzymes ASA
Publication of CA3092916A1 publication Critical patent/CA3092916A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA3092916A 2018-03-07 2019-03-07 Thermolabile proteinases Pending CA3092916A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1803654.1 2018-03-07
GBGB1803654.1A GB201803654D0 (en) 2018-03-07 2018-03-07 Thermolabile proteinases
PCT/EP2019/055724 WO2019170809A1 (en) 2018-03-07 2019-03-07 Thermolabile proteinases

Publications (1)

Publication Number Publication Date
CA3092916A1 true CA3092916A1 (en) 2019-09-12

Family

ID=61972698

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3092916A Pending CA3092916A1 (en) 2018-03-07 2019-03-07 Thermolabile proteinases

Country Status (10)

Country Link
US (1) US11591584B2 (https=)
EP (1) EP3762490A1 (https=)
JP (3) JP2021516993A (https=)
KR (1) KR20200142001A (https=)
CN (1) CN112105730B (https=)
BR (1) BR112020018050A2 (https=)
CA (1) CA3092916A1 (https=)
GB (1) GB201803654D0 (https=)
WO (1) WO2019170809A1 (https=)
ZA (1) ZA202006186B (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL244825B1 (pl) 2021-11-26 2024-03-11 Blirt Spolka Akcyjna Mutant proteinazy K Tritirachium album i jej zymogenu, plazmid ekspresyjny, rekombinantowy szczep Pichia pastoris i sposób wytwarzania dojrzałej formy mutanta proteinazy K

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3012377B2 (ja) * 1991-10-11 2000-02-21 名糖産業株式会社 低耐熱性プロテアーゼとその関連物及びその生産法
JPH06261752A (ja) * 1993-03-17 1994-09-20 Amano Pharmaceut Co Ltd 新規セリンプロテイナーゼ及びその製造法
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
JP3664804B2 (ja) * 1996-05-10 2005-06-29 花王株式会社 低温アルカリプロテアーゼｘ、それを生産する微生物、その製造法、並びに当該酵素を含有する洗浄剤組成物及び食品加工用酵素製剤
US5981235A (en) * 1996-07-29 1999-11-09 Promega Corporation Methods for isolating nucleic acids using alkaline protease
EP1476573B1 (en) 2002-01-28 2010-01-06 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
EP1769240B1 (en) * 2004-06-17 2011-11-09 ZyGEM Corporation Limited Purification methods and uses thereof
DE102005028295A1 (de) 2005-06-18 2006-11-16 Henkel Kgaa Proteasen aus psychrophilen Organismen
EP2147119B1 (en) 2007-05-03 2011-05-25 Roche Diagnostics GmbH SINGLE-CELL mRNA QUANTIFICATION WITH REAL-TIME RT-PCR
JP5283154B2 (ja) * 2008-01-22 2013-09-04 長瀬産業株式会社 トリプシン様酵素
ES2539856T3 (es) 2008-08-01 2015-07-06 F. Hoffmann-La Roche Ag Lisis mejorada y transcripción reversa para la cuantificación de mRNA
CN101586086B (zh) * 2009-05-15 2011-05-04 南京工业大学 一种耐有机溶剂蛋白酶及其产生菌株
JP2016036327A (ja) * 2014-08-11 2016-03-22 国立大学法人福島大学 プロテアーゼ含有洗浄組成物及びプロテアーゼの安定化方法
CA3028612A1 (en) * 2015-07-06 2017-01-12 Uvic Industry Partnerships Inc. Variants of the subtilisin carlsberg polypeptide with decreased thermostability

Also Published As

Publication number Publication date
JP2024178230A (ja) 2024-12-24
WO2019170809A1 (en) 2019-09-12
CN112105730A (zh) 2020-12-18
GB201803654D0 (en) 2018-04-25
JP2021516993A (ja) 2021-07-15
JP2024160310A (ja) 2024-11-13
EP3762490A1 (en) 2021-01-13
CN112105730B (zh) 2024-10-18
BR112020018050A2 (pt) 2020-12-29
JP7854473B2 (ja) 2026-05-01
US20200407701A1 (en) 2020-12-31
ZA202006186B (en) 2021-10-27
US11591584B2 (en) 2023-02-28
KR20200142001A (ko) 2020-12-21

Similar Documents

Publication Publication Date Title
EP3670655B1 (en) Proteinases with improved properties
Cao et al. Identification of a myofibril-bound serine proteinase (MBSP) in the skeletal muscle of lizard fish Saurida wanieso which specifically cleaves the arginine site
US10767211B2 (en) Modified amadoriase reacting with fructosyl hexapeptide
DK2814954T3 (en) endonucleases
WO2006089259A1 (en) Enzymatic digestion of tissue
Ertan et al. A new broad specificity alkaline metalloprotease from a Pseudomonas sp. isolated from refrigerated milk: Role of calcium in improving enzyme productivity
JP7854473B2 (ja) 易熱性プロテイナーゼ
CAO et al. Purification and characterization of a myofibril‐bound serine proteinase from the skeletal muscle of silver carp
KR102390400B1 (ko) 당화 펩티드에 작용하는 아마도리아제를 이용한 HbA1c 측정법
Bozóki et al. A recombinant fusion protein-based, fluorescent protease assay for high throughput-compatible substrate screening
US10934530B2 (en) Amadoriase having improved specific activity
NZ511680A (en) Method for preparing nucleic acid or DNA samples and a DNA extraction process using thermophilic proteases
Chi et al. Effects of sodium dodecyl sulfate on the enzyme catalysis and conformation of a recombinant γ-glutamyltranspeptidase from Bacillus licheniformis
Mojallal-Tabatabei et al. Purification and biochemical characterization of angiotensin I-converting enzyme (ACE) from ostrich lung: The effect of 2, 2, 2-trifluoroethanol on ACE conformation and activity
Akiyama et al. Roles of homooligomerization and membrane association in ATPase and proteolytic activities of FtsH in vitro
Klomklao et al. Cationic trypsin: A predominant proteinase in Pacific saury (Cololabis saira) pyloric ceca
Okubo et al. Processing, catalytic activity and crystal structures of kumamolisin‐As with an engineered active site
Yang et al. Glu88 in the non-catalytic domain of acylpeptide hydrolase plays dual roles: charge neutralization for enzymatic activity and formation of salt bridge for thermodynamic stability
CN103305588A (zh) 使用了酶的测定方法
US20070190552A1 (en) Purification methods and uses thereof
Henehan et al. Approaches to avoid proteolysis during protein expression and purification
Saijo-Hamano et al. A new purification method for overproduced proteins sensitive to endogenous proteases
Okada et al. Slow unfolding pathway of hyperthermophilic Tk-RNase H2 examined by pulse proteolysis using the stable protease Tk-subtilisin
Ganesh et al. Comparisons of protein extraction procedures and quantification methods for the proteomic analysis of Gram-positive Paenibacillus sp. strain D9
KANG et al. EFFECT OF TWO POLAR ORGANIC‐AQUEOUS SOLVENT SYSTEMS ON THE STRUCTURE‐FUNCTION RELATIONSHIPS OF PROTEASES III. PAPAIN AND TRYPSIN

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20220812

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 6TH ANNIV.) - STANDARD

Year of fee payment: 6

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20250205

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT

Effective date: 20250205

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20250205

D15 Examination report completed

Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D15-D126 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: EXAMINER'S REPORT

Effective date: 20250306

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT RECEIVED - RESPONSE TO EXAMINER'S REQUISITION

Effective date: 20250704

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT

Effective date: 20250708

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT DETERMINED COMPLIANT

Effective date: 20250804

P13 Application amended

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P13-X000 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: APPLICATION AMENDED

Effective date: 20250804

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 7TH ANNIV.) - STANDARD

Year of fee payment: 7

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20260205

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20260205

D15 Examination report completed

Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D15-D126 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: EXAMINER'S REPORT

Effective date: 20260423