CN112089054A - 降血糖益生菌组合物 - Google Patents
降血糖益生菌组合物 Download PDFInfo
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- CN112089054A CN112089054A CN202011004485.3A CN202011004485A CN112089054A CN 112089054 A CN112089054 A CN 112089054A CN 202011004485 A CN202011004485 A CN 202011004485A CN 112089054 A CN112089054 A CN 112089054A
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- lactobacillus
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- cfu
- lactobacillus reuteri
- lactobacillus salivarius
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Abstract
本发明公开了降血糖益生菌组合物，属于益生菌的所有相关食品产业领域。它包括唾液乳杆菌、罗伊氏乳杆菌和苦瓜粉。本发明中降血糖益生菌组合物组合物，有着高效的单糖利用率，并能减少肠道黏膜细胞对葡糖糖、半乳糖、果糖的吸收，降低血糖浓度。同时，显著改善葡萄糖的代谢情况和血脂水平和含量，减轻二型糖尿病对肝脏和肾脏的损伤。是一种能长期安全服用的，降低机体对糖类物质的吸收与利用，提高糖类降解代谢，改善二型糖尿病对机体伤害之食品补充或药物。
Description
技术领域
本发明涉及降血糖益生菌组合物，属于益生菌的所有相关食品产业领域。
背景技术
根据世界卫生组织的数据，全球糖尿病（diabetes）疾病的发病率约占人口数量的10%，在部分地区甚至高达33%。导致糖尿病的病因有很多，包括遗传和环境因素，占非传染性慢性疾病死亡病例的3.5%（WHO, 2016）。科学证据表明，胰岛素（insulin）耐受导致机体炎症应激的增加，肠道菌群与环境、遗传因素的相互影响导致了糖尿病的发生（Cani P Det al，2005）。
人的肠胃道腔内平均每毫升含有1014个，超过5000种微生物。研究表明，肠道菌群组成与过敏、肠道炎性疾病、癌症、糖尿病、心血管疾病和血脂异常等情况有关（Holmes E,2011; Larsen N, 2010）。肠道微生物通过多种分子机制对2型糖尿病（type 2 diabetes，T2D）产生影响。T2D与促炎细胞因子、趋化因子和炎症蛋白水平升高有关。一些肠道微生物和微生物产物，特别是脂多糖（lipopolysaccharide，LPS）能够降低炎症反应，刺激产生抗炎细胞因子和趋化因子（Gurung M，2020）。另外，由于紧密连接蛋白表达减少，导致细菌脂多糖（LPS）易位，进而导致肠道通透性和黏膜免疫反应的增加，这可能导致代谢性内毒素血症和胰岛素抵抗（Cani PD, 2008; Secondulfo M, 2004）。肠道菌群还可能通过影响肝脏、肌肉和脂肪等主要代谢器官的葡萄糖稳态和胰岛素抵抗，以及通过影响糖的消化和控制这一过程的肠道激素的产生来影响T2D（Gurung M，2020）。研究表明，乳酸双歧杆菌可以增加糖原合成，降低与葡萄糖异生相关的肝脏基因表达；同时改善了葡萄糖转运体4 （glucosetransporter-4，GLUT4）对葡萄糖的转运和刺激胰岛素对葡萄糖的摄取（Kim SH，2014）。增加脂肪酸的氧化和能量消耗以及减少脂肪酸的合成可以改善肥胖，从而改善T2D（HoumardJA, 2008）。益生菌对肠道菌群的调节可以促进一些临床疾病的改善，也被认为是胰岛素抵抗治疗中有效的佐剂。（Moroti C, 2012）研究表明，链脲佐菌素（streptozotocin）诱导的糖尿病小鼠，联合使用益生元和二甲双胍（metformin），与单用二甲双胍相比，联合用药可改善空腹血糖、葡萄糖耐量和胰岛素抵抗（Zheng J, 2018）。在一项随机双盲试验中，二甲双胍治疗T2D患者4个月后，对肠道菌群有很强的影响；安慰剂组转换成二甲双胍继续治疗2个月后，对肠道菌群影响也很大。将二甲双胍治疗后的粪便菌群移植后无菌小鼠后，小鼠葡萄糖耐受得到改善（Wu H，2017）。
为了筛选对T2D有干预潜力的益生菌菌株，体外研究测试了20株天然乳酸菌菌株并评估他们对单糖的分解能力。结果表明，唾液乳杆菌Lactobacillus salivarius AP-32和罗伊氏乳杆菌Lactobacillus reuteri GL-104有着优秀的单糖（葡萄糖）和混合单糖（葡萄糖、果糖和半乳糖）利用率。AP-32和GL-104能够抑制转运蛋白SGLT1和GLUT5基因的表达，从而减少葡萄糖、半乳糖、果糖从肠道中被肠粘膜细胞吸收进入血液，降低血糖浓度。进一步的实验表明，AP-32和GL-104可显著改善糖尿病小鼠的葡萄糖代谢，改善糖尿病小鼠的血脂水平和含量。同时，AP-32和GL-104联合使用，可显著改善T2D对肝脏和肾脏的损伤（HsiehP S，2020）。
苦瓜是一种复杂的却又不同寻常的植物药物，它不仅被广泛用作食物，而且在治疗糖尿病、牛皮癣和感染、调节月经周期、治疗不孕症和诱导流产等疾病方面也有着悠久的历史。苦瓜中含有一种蛋白质，在结构和药理上与胰岛素相似，它通常被称为v-胰岛素。在一项小型研究中，9例患者(6例为少年期发病，1例为成熟期发病，2例为无症状型糖尿病)皮下注射v-胰岛素。5例健康和5例明显糖尿病患者作为对照组，给予安慰剂注射。治疗组的降糖效果在注射后30 - 60分钟开始，且血糖浓度达到高峰的时间也显著低于常规时间（Baldwa VS，1977）。苦瓜富含膳食纤维和其他生物活性化合物，给肥胖大鼠喂食高脂饮食（HFD）并用苦瓜粉（BMP）处理8周；与HFD组相比，BMP有效延缓体重增加和提高胰岛素敏感性，降低内毒素负荷；BMP改变了肠道菌群，也改变了代谢谱，包括有机酸、氨基酸和糖类的代谢；在肥胖小鼠中，BMP通过恢复肠道菌群和肠道代谢产物发挥抑制胰岛素抵抗和抗炎作用（Bai J，2018）。苦瓜的另一个特点是它可以减轻糖尿病并发症。糖尿病与肾脏、眼睛、神经和血管的不可逆转的功能和结构变化有关，而苦瓜在各种动物模型中可能减轻这些并发症的某些方面。在三高综合征（高血糖与高胰岛素血症、高甘油三酯血症和肥胖相关）大鼠模型中，苦瓜提取物喂养的试验组大鼠，与对照组大鼠相比，可防止高胰岛素血症和高血糖（Grover JK，2004）。
全世界有约十分之一的人口饱受糖尿病的困扰。科学证据表明，胰岛素（insulin）耐受导致机体炎症应激的增加，肠道菌群与环境、遗传因素的相互影响导致了糖尿病的发生。目前临床上对糖尿病的治疗方案，多为口服或者注射促胰岛素分泌剂、胰岛素类似物或者胰岛血糖素抑制剂等。长期药物的使用，会导致机体对胰岛素出现耐受，使得药物逐渐没有效果，更不能改善并发症对机体的危害。因此，迫切需要一个安全、有效、无耐药风险的针对2型糖尿病的解决方案。近年来，药物（例如二甲双胍）和肠道菌群的相互作用受到了极大的关注。通过益生菌、益生元改变肠道菌群可能更加安全有效地影响糖尿病药物的效果。
发明内容
本发明所要解决的技术问题在于：提供降血糖益生菌组合物，作为有效、安全、不复发改善机体二型糖尿病的食品补充或药物的方法，解决降血糖相关市场上急需解决的问题。
本发明所要解决的技术问题采取以下技术方案来实现：
降血糖益生菌组合物，包括唾液乳杆菌、罗伊氏乳杆菌合并苦瓜粉，有效量为每日合并补充100亿～500亿唾液乳杆菌、罗伊氏乳杆菌，即0.5*1010 cfu/day～2.5*1010 cfu/day的唾液乳杆菌菌量和0.5*1010 cfu/day～2.5*1010 cfu/day的罗伊氏乳杆菌菌量，每日0.5克～2.5克苦瓜粉。
作为优选实例，所述唾液乳杆菌为唾液乳杆菌AP-32、罗伊氏乳杆菌为罗伊氏乳杆菌GL-104。。
作为优选实例，所述唾液乳杆菌、罗伊氏乳杆菌合并苦瓜粉的有效量为每日补充100亿，即0.5*1010 cfu/day的唾液乳杆菌和0.5*1010 cfu/day的罗伊氏乳杆菌菌量以及每日0.5克苦瓜粉。
作为优选实例，所述唾液乳杆菌、罗伊氏乳杆菌合并苦瓜粉可进一步包括药学上可接受之载剂、赋形剂或稀释剂；并可进一步制成流体乳品、浓缩牛奶、优酪乳、酸乳、冷冻优格、乳杆菌发酵饮料、奶粉、冰淇淋、乳酪、干酪、豆浆、发酵豆浆、蔬果汁、果汁、运动饮料、甜点、果冻、糖果、婴儿食品、健康食品、动物饲料、中草药组合物或膳食补充品。
本发明的有益效果是：本发明中的唾液乳杆菌、罗伊氏乳杆菌合并苦瓜粉，通过益生菌有效降低血糖，并组合降血糖因子苦瓜粉。有着高效的单糖利用率，并能减少肠道黏膜细胞对葡糖糖、半乳糖、果糖的吸收，降低血糖浓度。同时，显著改善葡萄糖的代谢情况和血脂水平和含量，减轻二型糖尿病对肝脏和肾脏的损伤。是一种能长期安全服用的，降低机体对糖类物质的吸收与利用，提高糖类降解代谢，改善二型糖尿病对机体伤害之食品补充或药物。
具体实施方式
为了对本发明的技术手段、创作特征、达成目的与功效易于明白了解，下面结合具体实施例，进一步阐述本发明。
实施例一
降血糖益生菌组合物，包括唾液乳杆菌、罗伊氏乳杆菌合并苦瓜粉，所述唾液乳杆菌、罗伊氏乳杆菌合并苦瓜粉的有效量为每日补充100亿，即0.5*1010 cfu/day的唾液乳杆菌和0.5*1010 cfu/day的罗伊氏乳杆菌菌量以及每日0.5克苦瓜粉。
实施例二
降血糖益生菌组合物，包括唾液乳杆菌、罗伊氏乳杆菌合并苦瓜粉，所述唾液乳杆菌、罗伊氏乳杆菌合并苦瓜粉的有效量为每日补充100亿，即1.0*1010 cfu/day的唾液乳杆菌和1.0*1010 cfu/day的罗伊氏乳杆菌菌量以及每日1.0克苦瓜粉。
实施例三
降血糖益生菌组合物，包括唾液乳杆菌、罗伊氏乳杆菌合并苦瓜粉，所述唾液乳杆菌、罗伊氏乳杆菌合并苦瓜粉的有效量为每日补充100亿，即1.5*1010 cfu/day的唾液乳杆菌和1.5*1010 cfu/day的罗伊氏乳杆菌菌量以及每日1.5克苦瓜粉。
实施例四
降血糖益生菌组合物，包括唾液乳杆菌、罗伊氏乳杆菌合并苦瓜粉，所述唾液乳杆菌、罗伊氏乳杆菌合并苦瓜粉的有效量为每日补充100亿，即2.0*1010 cfu/day的唾液乳杆菌和2.0*1010 cfu/day的罗伊氏乳杆菌菌量以及每日2.0克苦瓜粉。
实施例五
降血糖益生菌组合物，包括唾液乳杆菌、罗伊氏乳杆菌合并苦瓜粉，所述唾液乳杆菌、罗伊氏乳杆菌合并苦瓜粉的有效量为每日补充100亿，即2.5*1010 cfu/day的唾液乳杆菌和2.5*1010 cfu/day的罗伊氏乳杆菌菌量以及每日2.5克苦瓜粉。
研究方法及进行步骤
1.临床试验
选取60名年龄在18-60岁的患有二型糖尿病患者，男女不限；
随机分为试验组50例和对照组10例，试验组按照上述五个实施例（每组10例）服用本品，对照组口服安慰剂一个月；
试验组连续试用一个月，并随后停止试用再追踪观察一个月；
试用期间不使用其它降血糖的中西药物及疗法，追踪观察试验者服用本品1天前、服用药物30天后和服用药物60天后空腹血糖指标。
疗效判断标准：
(1)显效: 服用药物30天后的空腹血糖指标显著低于服用本品1天前水平，服用药物60天后的空腹血糖指标与服用药物30天后的空腹血糖指标无显著性差异；
(2)有效: 服用药物30天后的空腹血糖指标显著低于服用本品1天前水平，服用药物60天后的空腹血糖指标与服用药物30天后的空腹血糖指标有显著性差异，并接近于服用本品1天前水平；
(3)无效: 服用药物30天后的空腹血糖指标与服用本品1天前水平无显著性差异；
按照以上降血糖疗效标准观察效果，结果表明，本发明的组合物能够显著改善二型糖尿病患者的血糖水平，相比降血糖药物，本组合物安全有效，有助于二型糖尿病的治疗和维护。
以上显示和描述了本发明的基本原理和主要特征和本发明的优点。本行业的技术人员应该了解，本发明不受上述实施例的限制，在不脱离本发明精神和范围的前提下，本发明还会有各种变化和改进，这些变化和改进都落入要求保护的本发明范围内。本发明要求保护范围由所附的权利要求书及其等效物界定。
Claims (4)
1.降血糖益生菌组合物，其特征在于：包括唾液乳杆菌、罗伊氏乳杆菌和苦瓜粉，有效量为每日合并补充100亿～500亿唾液乳杆菌、罗伊氏乳杆菌，即0.5*1010 cfu/day～2.5*1010 cfu/day的唾液乳杆菌菌量和0.5*1010 cfu/day～2.5*1010 cfu/day的罗伊氏乳杆菌菌量，每日0.5克～2.5克苦瓜粉。
2.根据权利要求1所述的降血糖益生菌组合物，其特征在于：所述唾液乳杆菌为唾液乳杆菌AP-32、罗伊氏乳杆菌为罗伊氏乳杆菌GL-104。
3.根据权利要求1所述的降血糖益生菌组合物，其特征在于：唾液乳杆菌、罗伊氏乳杆菌合并苦瓜粉的有效量为每日补充100亿，即0.5*1010 cfu/day的唾液乳杆菌和0.5*1010cfu/day的罗伊氏乳杆菌菌量以及每日0.5克苦瓜粉。
4.根据权利要求1所述的降血糖益生菌组合物，其特征在于：所述唾液乳杆菌、罗伊氏乳杆菌合并苦瓜粉可进一步包括药学上可接受之载剂、赋形剂或稀释剂；并可进一步制成流体乳品、浓缩牛奶、优酪乳、酸乳、冷冻优格、乳杆菌发酵饮料、奶粉、冰淇淋、乳酪、干酪、豆浆、发酵豆浆、蔬果汁、果汁、运动饮料、甜点、果冻、糖果、婴儿食品、健康食品、动物饲料、中草药组合物或膳食补充品。
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| CN115463176A (zh) * | 2022-09-20 | 2022-12-13 | 潍坊君薇生物科技有限责任公司 | 一种降血糖组合物、制备方法及其应用 |
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