CN112063563A - Preparation method and use method of livestock and poultry manure deodorizing composite microbial inoculum - Google Patents

Preparation method and use method of livestock and poultry manure deodorizing composite microbial inoculum Download PDF

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CN112063563A
CN112063563A CN202011002574.4A CN202011002574A CN112063563A CN 112063563 A CN112063563 A CN 112063563A CN 202011002574 A CN202011002574 A CN 202011002574A CN 112063563 A CN112063563 A CN 112063563A
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fermentation
microbial inoculum
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deodorizing
livestock
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孙亚运
孙秀峰
王丹
房英伟
孙金刚
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Anhui Tongqiang Agricultural Co ltd
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Anhui Tongqiang Agricultural Co ltd
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F11/00—Treatment of sludge; Devices therefor
    • C02F11/02—Biological treatment
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00—Specific treatment goals
    • C02F2303/02—Odour removal or prevention of malodour

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Abstract

The invention discloses a preparation method of a livestock and poultry manure deodorizing composite microbial inoculum, which comprises the following steps: respectively adding bacillus amyloliquefaciens, alcaligenes, arthrobacter, streptomycete, micromonospora and mucor into a fermentation chamber in a fermentation box through a feed inlet of the fermentation chamber for fermentation; opening a discharge port on the fermentation chamber to enable strain fermentation liquor to flow out and be collected, then mixing six kinds of strain fermentation liquor, and preparing the deodorizing composite microbial inoculum; adding the deodorizing composite microbial inoculum into a culture medium for culturing; meanwhile, adding a tea polyphenol activating agent and a trehalose protecting agent into the culture medium, and uniformly stirring to obtain the livestock manure deodorizing composite microbial inoculum; the method for preparing the livestock excrement deodorizing composite microbial inoculum and the method for using the livestock excrement deodorizing composite microbial inoculum have the advantages of simple process and easy operation.

Description

Preparation method and use method of livestock and poultry manure deodorizing composite microbial inoculum
Technical Field
The invention belongs to the technical field of livestock and poultry, relates to a preparation method and a use method of a deodorizing complex microbial inoculum, and particularly relates to a preparation method and a use method of a deodorizing complex microbial inoculum for livestock and poultry manure.
Background
A large amount of livestock and poultry manure is stacked, so that the land is occupied, the resource waste is caused, and harmful gases such as hydrogen sulfide, ammonia gas and the like are generated, so that the environment is polluted, and the human health is seriously harmed.
A comparison document CN108913626A discloses a livestock and poultry excrement deodorizing composite microbial agent and a preparation method thereof, relates to the field of microorganism application, and is provided based on the problems of high cost, complex reaction, secondary pollution, poor adaptability and single action of a commercial biological deodorant based on a chemical deodorizing technology. The deodorizing composite microbial agent for the livestock and poultry manure comprises five bacteria of bacillus amyloliquefaciens, alcaligenes, arthrobacter, streptomycete and micromonospora; the preparation method comprises single strain fermentation, composite microbial inoculum preparation, and fresh culture medium, activating agent and protective agent addition. The invention has the beneficial effects that: improves the processing speed and the utilization rate of the excrement, and has quick and lasting deodorization effect.
In the prior art, the preparation efficiency of the livestock manure deodorizing complex microbial inoculum is low in the preparation process, and the effects of the livestock manure deodorizing complex microbial inoculum are not obvious and slow in use.
Disclosure of Invention
The invention aims to solve the problems that in the prior art, the preparation efficiency is low in the preparation process of the livestock manure deodorizing composite microbial inoculum and the effects are not obvious and slow in the use process of the livestock manure deodorizing composite microbial inoculum, and provides a preparation method and a use method of the livestock manure deodorizing composite microbial inoculum.
The purpose of the invention can be realized by the following technical scheme:
a preparation method of a livestock and poultry manure deodorizing complex microbial inoculum comprises the following steps:
s1, respectively adding bacillus amyloliquefaciens, alcaligenes, arthrobacter, streptomycete, micromonospora and mucor into a fermentation chamber in a fermentation box through a feed inlet of the fermentation chamber for fermentation, so that the six strains of bacillus amyloliquefaciens, alcaligenes, arthrobacter, streptomycete, micromonospora and mucor are simultaneously fermented in different fermentation chambers; during fermentation, the driving motor is controlled to rotate forwards and backwards to drive the transmission gear to rotate, and the side wall of the bottom surface of the second arc-shaped plate is provided with the rack which is meshed and connected with the transmission gear, so that the mounting frame rotates back and forth along the base, and the bacillus amyloliquefaciens, the alcaligenes, the arthrobacter, the streptomyces, the micromonospora and the mucor are respectively rocked and fermented in different fermentation chambers;
s2, after fermentation of six strains of bacillus amyloliquefaciens, alcaligenes, arthrobacter, streptomyces, micromonospora and mucor is finished, opening a discharge port on a fermentation chamber to enable strain fermentation liquor to flow out and be collected, then mixing the six strain fermentation liquor, and preparing the deodorizing composite microbial inoculum;
s3, adding the deodorizing composite bacterial agent into a culture medium for culture; meanwhile, adding a tea polyphenol activating agent and a trehalose protecting agent into the culture medium, and uniformly stirring to obtain the livestock manure deodorizing composite microbial inoculum.
Preferably, the installing frame is U type structure, is provided with the third transfer roller on the bottom inner wall of installing frame, and the both sides inner wall of installing frame is provided with first transfer roller and second transfer roller respectively, and the fermenting case setting is between first transfer roller, second transfer roller and third transfer roller.
Preferably, the installing frame is connected with the base through the subassembly that rolls, and the subassembly that rolls includes first arc, limiting plate, first spacing wheel, the spacing wheel of second, second arc, rack, driving motor, drive gear, limiting gear, and first arc is provided with two sets ofly side by side, and the one end of first arc is connected with the lateral wall top of installing frame, and the mid-portion of first arc is connected on one side of installing frame, and the other end of first arc is connected with the opposite side of installing frame bottom surface.
Preferably, the two sides of the top surface of the base are respectively provided with a first limiting wheel, a limiting plate is arranged between the two sets of first limiting wheels, a second limiting wheel is installed on the limiting plate, the two sets of first limiting wheels are located on the bottom surface of the first arc-shaped plate and are connected with the bottom surface of the first arc-shaped plate in a rotating mode, and the second limiting wheel is located on the top surface of the first arc-shaped plate and is connected with the top surface of the first arc-shaped plate in a rotating mode.
Preferably, a second arc is arranged between the two sets of first limiting wheels, one end of the second arc is connected with the top of the side wall of the mounting frame, the middle of the second arc is connected to one side of the mounting frame, the other end of the second arc is connected with the other side of the bottom surface of the mounting frame, a rack is arranged on the side wall of the bottom surface of the second arc, the rack is meshed with a transmission gear, the transmission gear is connected with the output end of a driving motor, the driving motor is mounted on the top surface of the base, limiting gears are arranged on the two sides of the transmission gear, the limiting gears are meshed with the rack on the second arc and mounted on the base.
Preferably, a plurality of groups of partition plates are arranged in the fermentation box at equal intervals, the fermentation box is divided into a plurality of groups of fermentation chambers through the partition plates, a feed inlet is formed in one side of the top surface of each group of fermentation chambers, and a discharge outlet is formed in the other side of the top surface of each group of fermentation chambers.
A use method of the livestock and poultry manure deodorizing complex microbial inoculum comprises the following steps:
s1, diluting the obtained livestock and poultry manure deodorizing composite microbial inoculum by water, adding the diluted microbial inoculum into a feed box, moving the microbial inoculum along a slide rail through a limiting roller at the bottom of the feed box, starting a pump body in the feed box to work, enabling the pump body to enable the livestock and poultry manure deodorizing composite microbial inoculum liquid to enter a spray plate along a connecting hose, spraying the liquid to feeding chambers at two sides of a passageway from spray holes of the spray plate, and deodorizing and sterilizing the livestock and poultry manure in the feeding chambers;
s2, in the sterilization process, the double-shaft motor is started to rotate forwards and backwards, the output end of the double-shaft motor is connected with the lead screw, the lead screw penetrates through the sliding block and is in threaded connection with the sliding block, the bottom surface of the sliding block is in sliding connection with the top surface of the feeding box, the top surface of the sliding block is in movable connection with one end of the movable rod, the other end of the movable rod is in movable connection with the side wall of the spraying plate, the spraying plate rotates along the feeding box, the feeding chamber is comprehensively sprayed, and then excrement and urine are turned and stirred and nest reaction treatment is carried out.
Preferably, a passageway is reserved at the feeding chamber, a sliding rail matched with the limiting idler wheels is arranged on the passageway, the limiting idler wheels are arranged on two sides of the bottom surface of the feed box, and a spraying mechanism is arranged on the top surface of the feed box.
Preferably, the spraying mechanism comprises a double-shaft motor, a screw rod, a sliding block, a movable rod, a connecting hose, a spraying plate and spraying holes, the double-shaft motor is installed on the top surface of the material box, the output end of the double-shaft motor is connected with the screw rod, the screw rod penetrates through the sliding block and is in threaded connection with the sliding block, the bottom surface of the sliding block is in sliding connection with the top surface of the material box, the top surface of the sliding block is movably connected with one end of the movable rod, the other end of the movable rod is movably connected with the side wall of the spraying plate, two sides of the top surface of the material box are respectively movably installed with the bottom surface of the spraying plate;
the two ends of the side wall of the spraying plate, which are close to the movable rod, are respectively connected with the discharge end of the connecting hose, and the feed end of the connecting hose extends to the inside of the material box and is connected with the output end of the pump body.
Compared with the prior art, the invention has the beneficial effects that: respectively adding bacillus amyloliquefaciens, alcaligenes, arthrobacter, streptomycete, micromonospora and mucor into a fermentation chamber in a fermentation box through a charging hole of the fermentation chamber for fermentation, so that six strains of bacillus amyloliquefaciens, alcaligenes, arthrobacter, streptomycete, micromonospora and mucor are simultaneously fermented in different fermentation chambers; during fermentation, the driving motor is controlled to rotate forwards and backwards to drive the transmission gear to rotate, and the side wall of the bottom surface of the second arc-shaped plate is provided with the rack which is meshed and connected with the transmission gear, so that the mounting frame rotates back and forth along the base, and the bacillus amyloliquefaciens, the alcaligenes, the arthrobacter, the streptomyces, the micromonospora and the mucor are respectively rocked and fermented in different fermentation chambers; the fermentation box can simultaneously ferment six strains, greatly improves the preparation efficiency of the livestock manure deodorizing composite microbial inoculum, and simultaneously, the strains can be effectively fermented by arranging the rolling component, so that the preparation efficiency of the livestock manure deodorizing composite microbial inoculum is further improved;
after the fermentation of six strains of bacillus amyloliquefaciens, alcaligenes, arthrobacter, streptomycete, micromonospora and mucor is finished, opening a discharge port on a fermentation chamber to enable strain fermentation liquor to flow out and be collected, then mixing the six strain fermentation liquor, and preparing the deodorizing composite microbial inoculum;
adding the deodorizing composite microbial inoculum into a culture medium for culturing; meanwhile, the tea polyphenol activator and the trehalose protective agent are added into the culture medium and stirred uniformly to obtain the livestock manure deodorizing composite microbial inoculum, and the obtained livestock manure deodorizing composite microbial inoculum has the advantages of stable structure, high viable count and quick and lasting deodorizing effect by utilizing the synergistic effect of the six strains; the problems that the manure and the urine need to be separated in the traditional livestock manure composting treatment, the traditional deodorizing microbial inoculum has unobvious effect and takes effect slowly are solved, and the manure treatment speed and the manure utilization rate are improved; meanwhile, in the preparation process, the arranged fermentation box is matched with the rolling component, so that the preparation efficiency of the livestock and poultry manure deodorizing composite microbial inoculum is greatly improved; meanwhile, tea polyphenol is added in the preparation process, and interacts with microbial strains to inhibit the growth of mixed bacteria, so that odorous gas in the environment can be eliminated for a long time, and the preparation method has no other pollution and has good ecological benefit and economic benefit;
diluting the obtained livestock and poultry excrement deodorizing composite microbial inoculum by using water, adding the diluted livestock and poultry excrement deodorizing composite microbial inoculum into a feed box, moving the feed box along a slide rail through a limiting roller at the bottom of the feed box, starting a pump body in the feed box to work, enabling the pump body to enable the livestock and poultry excrement deodorizing composite microbial inoculum liquid to enter a spray plate along a connecting hose, spraying the livestock and poultry excrement deodorizing composite microbial inoculum liquid to feeding chambers on two sides of a passageway from spray holes of the spray plate, and deodorizing and sterilizing the livestock and poultry excrement in the feeding chambers;
in the sterilization process, the double-shaft motor is started to rotate forwards and backwards, the output end of the double-shaft motor is connected with the lead screw, the lead screw penetrates through the sliding block and is in threaded connection with the sliding block, the bottom surface of the sliding block is in sliding connection with the top surface of the feed box, the top surface of the sliding block is movably connected with one end of the movable rod, the other end of the movable rod is movably connected with the side wall of the spraying plate, the spraying plate is enabled to rotate along the feed box, the feeding chamber is comprehensively sprayed, and then excrement is turned and stirred and subjected to pit reactor reaction treatment; when the livestock excrement deodorizing composite microbial inoculum is used, the livestock excrement deodorizing composite microbial inoculum can be uniformly and comprehensively sprayed in a feeding room for reaction treatment by virtue of a feed box and a spraying mechanism on the feed box, so that the use efficiency of the livestock excrement deodorizing composite microbial inoculum is improved;
the method for preparing the livestock excrement deodorizing composite microbial inoculum and the method for using the livestock excrement deodorizing composite microbial inoculum have the advantages of simple process and easy operation.
Drawings
In order to facilitate understanding for those skilled in the art, the present invention will be further described with reference to the accompanying drawings.
Fig. 1 is a perspective view of the tumbling assembly of the present invention.
Fig. 2 is a side view of the tumbling assembly of the present invention.
Fig. 3 is a bottom view of the tumbling assembly of the present invention.
FIG. 4 is a schematic view of the structure of the fermenter according to the present invention.
Fig. 5 is a schematic structural view of a spraying mechanism of the present invention.
In the figure: 1. a base; 2. installing a frame; 3. a fermentation box; 4. a first arc-shaped plate; 5. a limiting plate; 6. a first limit wheel; 7. a second limiting wheel; 8. a first transfer roller; 9. a second transfer roller; 10. a third transfer roller; 11. a partition plate; 12. a second arc-shaped plate; 13. a rack; 14. a drive motor; 15. a transmission gear; 16. a limit gear; 17. a fermentation chamber; 18. a feed inlet; 19. a discharge port; 20. a material box; 21. a feeding room; 22. limiting the idler wheel; 23. a double-shaft motor; 24. a screw rod; 25. a slider; 26. a movable rod; 27. a connecting hose; 28. a spray plate; 29. spraying holes; 30. a slide rail.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, a method for preparing a composite microbial agent for deodorizing livestock and poultry manure comprises the following steps:
s1, respectively adding bacillus amyloliquefaciens, alcaligenes, arthrobacter, streptomycete, micromonospora and mucor into the fermentation chamber 17 in the fermentation box 3 through the feed inlet 18 of the fermentation chamber 17 for fermentation, so that the six strains of bacillus amyloliquefaciens, alcaligenes, arthrobacter, streptomycete, micromonospora and mucor are simultaneously fermented in different fermentation chambers 17; during fermentation, the driving motor 14 is controlled to rotate forwards and backwards to drive the transmission gear 15 to rotate, and as the rack 13 is arranged on the side wall of the bottom surface of the second arc-shaped plate 12 and the rack 13 is meshed and connected with the transmission gear 15, the mounting frame 2 rotates back and forth along the base 1, so that the bacillus amyloliquefaciens, the alcaligenes, the arthrobacter, the streptomyces, the micromonospora and the mucor are respectively rocked and fermented in different fermentation chambers 17; the fermentation box 3 can simultaneously ferment six strains, so that the preparation efficiency of the livestock manure deodorizing composite microbial inoculum is greatly improved, and meanwhile, the strains can be effectively fermented by arranging the rolling component, so that the preparation efficiency of the livestock manure deodorizing composite microbial inoculum is further improved;
s2, after fermentation of six strains of bacillus amyloliquefaciens, alcaligenes, arthrobacter, streptomyces, micromonospora and mucor is finished, opening a discharge port 19 on a fermentation chamber 17 to enable strain fermentation liquor to flow out and be collected, then mixing the six strain fermentation liquor, and preparing the deodorizing composite microbial inoculum;
s3, adding the deodorizing composite bacterial agent into a culture medium for culture; meanwhile, adding a tea polyphenol activating agent and a trehalose protecting agent into the culture medium, and uniformly stirring to obtain the livestock manure deodorizing composite microbial inoculum.
The installing frame 2 is U type structure, is provided with third transfer roller 10 on the bottom inner wall of installing frame 2, and the both sides inner wall of installing frame 2 is provided with first transfer roller 8 and second transfer roller 9 respectively, and fermenting case 3 sets up between first transfer roller 8, second transfer roller 9 and third transfer roller 10.
Installing frame 2 is connected with base 1 through the subassembly that rolls, the subassembly that rolls includes first arc 4, limiting plate 5, first spacing round 6, spacing round 7 of second, second arc 12, rack 13, driving motor 14, drive gear 15, stop gear 16, first arc 4 is provided with two sets ofly side by side, the one end of first arc 4 is connected with the lateral wall top of installing frame 2, the mid-connection of first arc 4 is on one side of installing frame 2, the other end of first arc 4 is connected with the opposite side of installing frame 2 bottom surface.
The top surface both sides of base 1 are provided with first spacing round 6 respectively, are provided with limiting plate 5 between two sets of first spacing rounds 6, install the spacing round 7 of second on the limiting plate 5, and two sets of first spacing rounds 6 are located the bottom surface of first arc 4 to rotate with the bottom surface of first arc 4 and be connected, and the spacing round 7 of second is located the top surface of first arc 4, and rotates with the top surface of first arc 4 and be connected.
Be provided with second arc 12 between two sets of first spacing wheels 6, the one end of second arc 12 is connected with the lateral wall top of installing frame 2, the middle part of second arc 12 is connected on one side of installing frame 2, the other end of second arc 12 is connected with the opposite side of installing frame 2 bottom surface, be provided with rack 13 on the bottom surface lateral wall of second arc 12, rack 13 is connected with drive gear 15 meshing, drive gear 15 is connected with driving motor 14's output, driving motor 14 installs on the top surface of base 1, the both sides of drive gear 15 are provided with limit gear 16, limit gear 16 is connected with the rack 13 meshing on the second arc 12, limit gear 16 installs on base 1.
A plurality of groups of partition plates 11 are arranged in the fermentation box 3 at equal intervals, the fermentation box 3 is divided into a plurality of groups of fermentation chambers 17 through the partition plates 11, a feeding port 18 is arranged on one side of the top surface of each group of fermentation chambers 17, and a discharging port 19 is arranged on the other side of the top surface of each group of fermentation chambers 17.
A use method of the livestock and poultry manure deodorizing complex microbial inoculum comprises the following steps:
s1, diluting the obtained livestock manure deodorizing composite microbial inoculum by 100 times with water, then adding the diluted bacteria into a feed box 20, moving along a slide rail 30 through a limiting roller 22 at the bottom of the feed box 20, starting a pump body in the feed box 20 to work, enabling the pump body to enable the livestock manure deodorizing composite microbial inoculum liquid to enter a spraying plate 28 along a connecting hose 27, then spraying the livestock manure deodorizing composite microbial inoculum liquid to the feeding chambers 21 at two sides of a passageway from spraying holes 29 of the spraying plate 28, and deodorizing and sterilizing the livestock manure in the feeding chambers 21;
s2, in the sterilization process, the double-shaft motor 23 is started to rotate forwards and backwards, the output end of the double-shaft motor 23 is connected with the lead screw 24, the lead screw 24 penetrates through the sliding block 25 and is in threaded connection with the sliding block 25, the bottom surface of the sliding block 25 is in sliding connection with the top surface of the feed box 20, the top surface of the sliding block 25 is movably connected with one end of the movable rod 26, the other end of the movable rod 26 is movably connected with the side wall of the spraying plate 28, the spraying plate 28 rotates along the feed box 20, the feeding chamber 21 is comprehensively sprayed, and then excrement is turned and mixed and nest stacking reaction treatment is carried out.
A passageway is reserved in the feeding chamber 21, a sliding rail 30 matched with the limiting idler wheels 22 is arranged on the passageway, the limiting idler wheels 22 are arranged on two sides of the bottom surface of the feed box 20, and a spraying mechanism is arranged on the top surface of the feed box 20.
The spraying mechanism comprises a double-shaft motor 23, a screw rod 24, a sliding block 25, a movable rod 26, a connecting hose 27, a spraying plate 28 and spraying holes 29, the double-shaft motor 23 is installed on the top surface of the material box 20, the output end of the double-shaft motor 23 is connected with the screw rod 24, the screw rod 24 penetrates through the sliding block 25 and is in threaded connection with the sliding block 25, the bottom surface of the sliding block 25 is in sliding connection with the top surface of the material box 20, the top surface of the sliding block 25 is movably connected with one end of the movable rod 26, the other end of the movable rod 26 is movably connected with the side wall of the spraying plate 28, two sides of the top surface of the material box 20 are respectively movably installed with the bottom surface of the spraying plate 28, and;
the two ends of the side wall of the spray plate 28 close to the movable rod 26 are respectively connected with the discharge end of the connecting hose 27, and the feed end of the connecting hose 27 extends to the inside of the feed box 20 and is connected with the output end of the pump body.
The working principle of the invention is as follows: respectively adding bacillus amyloliquefaciens, alcaligenes, arthrobacter, streptomycete, micromonospora and mucor into a fermentation chamber 17 in a fermentation box 3 through a feed inlet 18 of the fermentation chamber 17 for fermentation, so that six strains of the bacillus amyloliquefaciens, the alcaligenes, the arthrobacter, the streptomycete, the micromonospora and the mucor are simultaneously fermented in different fermentation chambers 17; during fermentation, the driving motor 14 is controlled to rotate forwards and backwards to drive the transmission gear 15 to rotate, and as the rack 13 is arranged on the side wall of the bottom surface of the second arc-shaped plate 12 and the rack 13 is meshed and connected with the transmission gear 15, the mounting frame 2 rotates back and forth along the base 1, so that the bacillus amyloliquefaciens, the alcaligenes, the arthrobacter, the streptomyces, the micromonospora and the mucor are respectively rocked and fermented in different fermentation chambers 17; the fermentation box 3 can simultaneously ferment six strains, so that the preparation efficiency of the livestock manure deodorizing composite microbial inoculum is greatly improved, and meanwhile, the strains can be effectively fermented by arranging the rolling component, so that the preparation efficiency of the livestock manure deodorizing composite microbial inoculum is further improved;
after the fermentation of six strains of bacillus amyloliquefaciens, alcaligenes, arthrobacter, streptomycete, micromonospora and mucor is finished, opening a discharge port 19 on a fermentation chamber 17 to enable strain fermentation liquor to flow out and be collected, then mixing the six strain fermentation liquor, and preparing the deodorizing composite microbial inoculum;
adding the deodorizing composite microbial inoculum into a culture medium for culturing; meanwhile, the tea polyphenol activator and the trehalose protective agent are added into the culture medium and stirred uniformly to obtain the livestock manure deodorizing composite microbial inoculum, and the obtained livestock manure deodorizing composite microbial inoculum has the advantages of stable structure, high viable count and quick and lasting deodorizing effect by utilizing the synergistic effect of the six strains; the problems that the manure and the urine need to be separated in the traditional livestock manure composting treatment, the traditional deodorizing microbial inoculum has unobvious effect and takes effect slowly are solved, and the manure treatment speed and the manure utilization rate are improved; meanwhile, in the preparation process, the fermentation box 3 is matched with the rolling component, so that the preparation efficiency of the livestock manure deodorizing composite microbial inoculum is greatly improved; meanwhile, tea polyphenol is added in the preparation process, and interacts with microbial strains to inhibit the growth of mixed bacteria, so that odorous gas in the environment can be eliminated for a long time, and the preparation method has no other pollution and has good ecological benefit and economic benefit;
diluting the obtained livestock manure deodorizing composite microbial inoculum by 100 times with water, adding the diluted bacteria into a feed box 20, moving the bacteria along a slide rail 30 through a limiting roller 22 at the bottom of the feed box 20, starting a pump body in the feed box 20 to work, enabling the pump body to enable the livestock manure deodorizing composite microbial inoculum liquid to enter a spray plate 28 along a connecting hose 27, then spraying the bacteria to a feeding chamber 21 at two sides of a passageway from spray holes 29 of the spray plate 28, and deodorizing and sterilizing the livestock manure in the feeding chamber 21;
in the sterilization process, the double-shaft motor 23 is started to rotate forwards and backwards, the output end of the double-shaft motor 23 is connected with the screw rod 24, the screw rod 24 penetrates through the sliding block 25 and is in threaded connection with the sliding block 25, the bottom surface of the sliding block 25 is in sliding connection with the top surface of the feed box 20, the top surface of the sliding block 25 is movably connected with one end of the movable rod 26, the other end of the movable rod 26 is movably connected with the side wall of the spraying plate 28, the spraying plate 28 rotates along the feed box 20, so that the feeding chamber 21 is sprayed comprehensively, and then excrement is turned and stirred and subjected to pit reaction treatment; when the livestock excrement deodorizing composite microbial inoculum is used, the livestock excrement deodorizing composite microbial inoculum can be uniformly and comprehensively sprayed in the feeding room 21 for reaction treatment by virtue of the feed box 20 and the spraying mechanism on the feed box 20, so that the use efficiency of the livestock excrement deodorizing composite microbial inoculum is improved;
the method for preparing the livestock excrement deodorizing composite microbial inoculum and the method for using the livestock excrement deodorizing composite microbial inoculum have the advantages of simple process and easy operation.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise forms disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims (9)

1. A preparation method of a composite microbial inoculum for deodorizing livestock and poultry manure is characterized by comprising the following steps: the method comprises the following steps:
s1, adding bacillus amyloliquefaciens, alcaligenes, arthrobacter, streptomycete, micromonospora and mucor into a fermentation chamber (17) in a fermentation box (3) through a feed inlet (18) of the fermentation chamber (17) respectively for fermentation, so that six strains of bacillus amyloliquefaciens, alcaligenes, arthrobacter, streptomycete, micromonospora and mucor are fermented simultaneously in different fermentation chambers (17); during fermentation, the driving motor (14) is controlled to rotate forwards and backwards to drive the transmission gear (15) to rotate, and as the rack (13) is arranged on the side wall of the bottom surface of the second arc-shaped plate (12), and the rack (13) is meshed and connected with the transmission gear (15), the mounting frame (2) rotates back and forth along the base (1), so that the bacillus amyloliquefaciens, the alcaligenes, the arthrobacter, the streptomyces, the micromonospora and the mucor are respectively rocked and fermented in different fermentation chambers (17);
s2, after fermentation of six strains of bacillus amyloliquefaciens, alcaligenes, arthrobacter, streptomyces, micromonospora and mucor is finished, opening a discharge hole (19) on a fermentation chamber (17) to enable strain fermentation liquor to flow out and be collected, then mixing the six strain fermentation liquor, and preparing the deodorizing composite microbial inoculum;
s3, adding the deodorizing composite bacterial agent into a culture medium for culture; meanwhile, adding a tea polyphenol activating agent and a trehalose protecting agent into the culture medium, and uniformly stirring to obtain the livestock manure deodorizing composite microbial inoculum.
2. The preparation method of the composite microbial inoculum for deodorizing livestock manure according to claim 1, wherein the mounting frame (2) is of a U-shaped structure, the bottom inner wall of the mounting frame (2) is provided with a third conveying roller (10), the inner walls of two sides of the mounting frame (2) are respectively provided with a first conveying roller (8) and a second conveying roller (9), and the fermentation box (3) is arranged among the first conveying roller (8), the second conveying roller (9) and the third conveying roller (10).
3. The preparation method of the poultry and livestock manure deodorizing composite microbial inoculum according to claim 1, characterized in that the mounting frame (2) is connected with the base (1) through a rolling assembly, the rolling assembly comprises a first arc-shaped plate (4), a limiting plate (5), a first limiting wheel (6), a second limiting wheel (7), a second arc-shaped plate (12), a rack (13), a driving motor (14), a transmission gear (15) and a limiting gear (16), two groups of the first arc-shaped plates (4) are arranged side by side, one end of each first arc-shaped plate (4) is connected with the top of the side wall of the mounting frame (2), the middle of each first arc-shaped plate (4) is connected to one side of the mounting frame (2), and the other end of each first arc-shaped plate (4) is connected with the other side of the bottom surface of the mounting frame (2).
4. The preparation method of the composite microbial inoculum for deodorizing the livestock and poultry manure according to claim 3, wherein two sides of the top surface of the base (1) are respectively provided with a first limiting wheel (6), a limiting plate (5) is arranged between two groups of the first limiting wheels (6), a second limiting wheel (7) is arranged on the limiting plate (5), the two groups of the first limiting wheels (6) are positioned on the bottom surface of the first arc-shaped plate (4) and are rotatably connected with the bottom surface of the first arc-shaped plate (4), and the second limiting wheel (7) is positioned on the top surface of the first arc-shaped plate (4) and is rotatably connected with the top surface of the first arc-shaped plate (4).
5. The preparation method of the composite microbial inoculum for deodorizing the livestock manure according to claim 4, characterized in that a second arc-shaped plate (12) is arranged between two groups of first limiting wheels (6), one end of the second arc-shaped plate (12) is connected with the top of the side wall of the mounting frame (2), the middle part of the second arc-shaped plate (12) is connected with one side of the mounting frame (2), the other end of the second arc-shaped plate (12) is connected with the other side of the bottom surface of the mounting frame (2), a rack (13) is arranged on the side wall of the bottom surface of the second arc-shaped plate (12), the rack (13) is meshed with a transmission gear (15), the transmission gear (15) is connected with the output end of a driving motor (14), the driving motor (14) is installed on the top surface of the base (1), limiting gears (16) are arranged on two sides of the transmission gear (15), the limiting gears (16) are meshed with the rack (13) on the second arc-shaped plate (, the limit gear (16) is arranged on the base (1).
6. The preparation method of the composite microbial inoculum for deodorizing livestock and poultry manure according to claim 1, characterized in that a plurality of groups of partition plates (11) are arranged in the fermentation box (3) at equal intervals, the fermentation box (3) is divided into a plurality of groups of fermentation chambers (17) through the partition plates (11), one side of the top surface of each group of fermentation chambers (17) is provided with a feeding port (18), and the other side of the top surface of each group of fermentation chambers (17) is provided with a discharging port (19).
7. A use method of the livestock and poultry manure deodorizing complex microbial inoculum is characterized by comprising the following steps:
s1, diluting the obtained livestock manure deodorizing composite bacterial agent by 100 times with water, adding the diluted bacterial agent into a feed box (20), moving along a slide rail (30) through a limiting roller (22) at the bottom of the feed box (20), starting a pump body in the feed box (20) to work, enabling the pump body to enable the livestock manure deodorizing composite bacterial agent liquid to enter a spraying plate (28) along a connecting hose (27), spraying the liquid onto a feeding room (21) at two sides of a passageway from spraying holes (29) of the spraying plate (28), and deodorizing and sterilizing the livestock manure in the feeding room (21);
s2, in the sterilization process, the double-shaft motor (23) is started to rotate forwards and backwards, the output end of the double-shaft motor (23) is connected with the screw rod (24), the screw rod (24) penetrates through the sliding block (25) and is in threaded connection with the sliding block (25), the bottom surface of the sliding block (25) is in sliding connection with the top surface of the feed box (20), the top surface of the sliding block (25) is movably connected with one end of the movable rod (26), the other end of the movable rod (26) is movably connected with the side wall of the spraying plate (28), the spraying plate (28) is enabled to rotate along the feed box (20), the feeding chamber (21) is fully sprayed, and then excrement is turned and stirred and is subjected to nest pile reaction treatment.
8. The use method of the livestock and poultry manure deodorizing composite microbial inoculum according to claim 7, characterized in that a passageway is reserved at the feeding chamber (21), a slide rail (30) matched with the limiting roller (22) is arranged on the passageway, the limiting roller (22) is arranged on two sides of the bottom surface of the feed box (20), and a spraying mechanism is arranged on the top surface of the feed box (20).
9. The use method of the composite microbial inoculum for deodorizing the livestock and poultry manure according to claim 8, characterized in that the spraying mechanism comprises a double-shaft motor (23), a screw rod (24), a slide block (25), a movable rod (26), a connecting hose (27), a spraying plate (28) and a spraying hole (29), the double-shaft motor (23) is arranged on the top surface of the feed box (20), the output end of the double-shaft motor (23) is connected with a screw rod (24), the screw rod (24) penetrates through a sliding block (25), the spraying device is connected with the sliding block (25) in a threaded manner, the bottom surface of the sliding block (25) is in sliding connection with the top surface of the material box (20), the top surface of the sliding block (25) is movably connected with one end of a movable rod (26), the other end of the movable rod (26) is movably connected with the side wall of a spraying plate (28), two sides of the top surface of the material box (20) are respectively movably installed with the bottom surface of the spraying plate (28), and a plurality of groups of spraying holes (29) are uniformly formed in the side wall, far away from the movable rod (26), of the spraying plate (28);
two ends of the side wall of the spray plate (28) close to the movable rod (26) are respectively connected with the discharge end of the connecting hose (27), and the feed end of the connecting hose (27) extends to the inside of the feed box (20) and is connected with the output end of the pump body.
CN202011002574.4A 2020-09-22 2020-09-22 Preparation method and use method of livestock and poultry manure deodorizing composite microbial inoculum Pending CN112063563A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013110133A1 (en) * 2012-01-27 2013-08-01 Gfs Corporation Aus Pty Ltd. Improved poultry farm practices
CN105255756A (en) * 2015-09-29 2016-01-20 北京市畜牧业环境监测站 Deodorant complex microbial inoculant and preparation method and application thereof
CN206980462U (en) * 2017-06-16 2018-02-09 北京智创环绿科技有限公司 A kind of composite bacteria agent sprays odor removal
CN108913626A (en) * 2018-07-26 2018-11-30 中国科学院合肥物质科学研究院 A kind of fowl and animal excrement deodorization composite bacteria agent and preparation method thereof
CN208517425U (en) * 2018-06-21 2019-02-19 河北土木启生物科技有限公司 A kind of process units of complex micro organism fungicide

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013110133A1 (en) * 2012-01-27 2013-08-01 Gfs Corporation Aus Pty Ltd. Improved poultry farm practices
CN105255756A (en) * 2015-09-29 2016-01-20 北京市畜牧业环境监测站 Deodorant complex microbial inoculant and preparation method and application thereof
CN206980462U (en) * 2017-06-16 2018-02-09 北京智创环绿科技有限公司 A kind of composite bacteria agent sprays odor removal
CN208517425U (en) * 2018-06-21 2019-02-19 河北土木启生物科技有限公司 A kind of process units of complex micro organism fungicide
CN108913626A (en) * 2018-07-26 2018-11-30 中国科学院合肥物质科学研究院 A kind of fowl and animal excrement deodorization composite bacteria agent and preparation method thereof

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