CN112023706A

CN112023706A - Adsorption membrane, composite membrane module and filter

Info

Publication number: CN112023706A
Application number: CN202011116401.5A
Authority: CN
Inventors: 卢洪洲; 张�林; 薛俊; 黄鹤平; 陈沈磊; 陆雪峰
Original assignee: Shanghai Taizi Biological Technology Co ltd
Current assignee: Shanghai Taizi Biological Technology Co ltd
Priority date: 2019-10-28
Filing date: 2020-10-19
Publication date: 2020-12-04
Also published as: CN110711493A

Abstract

The application provides an adsorption membrane, and a composite membrane component and a filter using the adsorption membrane, which are used for adsorbing HIV virus in blood plasma. The material of the adsorption film is lignin mixed with a plurality of particles with positive charges or negative charges, and the particles with positive charges or negative charges are used for adsorbing HIV virus or coronavirus; and, the adsorption film is a porous structure.

Description

Adsorption membrane, composite membrane module and filter

Technical Field

The application relates to the technical field of blood filtration, in particular to an adsorption membrane, and a composite membrane assembly and a filter using the adsorption membrane.

Background

The prevention and treatment of AIDS is a major research subject related to life health after 21 century. Governments in various countries pay great attention to and support for the prevention and treatment of AIDS, and various medicines and treatment methods for preventing and treating AIDS are continuously appeared.

At present, no report that AIDS virus (HIV) in blood of a patient is directly removed by a filtering method to achieve a treatment effect exists clinically in China. U.S. Pat. No. 6,168,718 discloses a method for reducing HIV in blood by filtration, but the method belongs to ultrafiltration method, and although it has a certain effect in reducing HIV, it also damages normal cells and proteins in human blood.

However, other existing aids virus filters are only suitable for small-dose filtration, and when a large amount of filtration is required, the filter membrane cannot be loaded, so that the situation that filtration cannot be realized is caused.

The Coronavirus belongs to the phylogenetic group of the family of the genus Nidovirales (Nidovirales) coronaviruses (Coronavir), is a single-stranded positive-sense RNA virus, and has certain similarity with HIV virus. Viruses of the genus coronaviruses are enveloped (envelope) RNA viruses with a linear single-stranded positive strand genome, and are a large group of viruses that are widely found in nature.

It has been shown that coronavirus, such as SARS-CoV envelope protein, may have a positive charge (Vicenzi E, Canduci F, Pinna D, et al. Coronavir and SARS-associated coronavirus strain HSR1. emulsifying infection Diseases, 2004, 10(3): 413-.

Therefore, a new filter is needed to overcome the above-mentioned drawbacks of the prior art and to filter hiv and coronavirus.

Disclosure of Invention

The purpose of the application is to provide an adsorption film, and a composite film assembly and a filter using the adsorption film, so as to remove HIV virus or coronavirus in plasma, and the adsorption film can specifically adsorb the HIV virus or coronavirus by mixing particles with positive charge or negative charge in lignin, so as to remove the HIV virus or coronavirus.

In order to achieve the above objects, the present application provides an adsorption film for adsorbing HIV virus or coronavirus, the adsorption film being made of a material of lignin doped with a plurality of positively or negatively charged microparticles for adsorbing HIV virus; and, the adsorption film is a porous structure.

In an embodiment of the application, the thickness of the filtering membrane is 3-5 mm.

In an embodiment of the present application, the pores of the porous structure are 0.1 to 0.6 μm.

In one embodiment of the present application, the adsorption film comprises positively or negatively charged particles, such as but not limited to silicon particles with positive or negative surface charge, so that the particles are 1+, 2+, 1-, or 2-. In one embodiment of the present application, the adsorbent membrane has specific adsorption for HIV virus or coronavirus.

The application also provides a composite membrane component for filtering HIV virus or coronavirus in blood plasma, which comprises at least one layer of adsorption membrane and at least one layer of terminal filtering membrane.

In one embodiment of the present application, the composite membrane module comprises at least one first adsorption membrane and at least one second adsorption membrane; wherein the pore diameter of the first adsorption membrane is 0.6 micron, and the pore diameter of the second adsorption membrane is 0.1-0.3 micron.

In a preferred embodiment of the present application, the composite membrane module includes four layers of the first adsorption membrane, one layer of the second adsorption membrane, and one layer of the end filtration membrane, which are sequentially stacked.

In an embodiment of the application, the terminal filtering membrane is of a porous structure, the pore diameter is 0.1-0.3 micrometers, and the material of the terminal filtering membrane is polyether sulfone.

The application also provides a filter for filtering HIV virus or coronavirus in blood plasma, which comprises a first shell and a second shell, wherein the first shell and the second shell are mutually clamped to form an accommodating space, and a composite membrane component is arranged in the accommodating space; the composite membrane assembly comprises at least one first adsorption membrane, at least one second adsorption membrane and a terminal filtering membrane which are laminated; wherein the content of the first and second substances,

the composite membrane assembly is formed along the direction of the plasma flow according to the arrangement mode of the first adsorption membrane, the second adsorption membrane and the terminal filtering membrane; and wherein the one or more of the one,

the first adsorption membrane, the second adsorption membrane and the terminal filtering membrane are all of porous structures, the aperture of the first adsorption membrane is 0.6 micrometer, the aperture of the second adsorption membrane is 0.1-0.3 micrometer, the aperture of the terminal filtering membrane is 0.1-0.3 micrometer, the first adsorption membrane and the second adsorption membrane are made of lignin mixed with a plurality of particles with positive charges or negative charges, and the terminal filtering membrane is made of polyether sulfone.

In an embodiment of the present application, the pressure difference between the first adsorption membrane and the final filtration membrane along the plasma flow direction is 0.3-0.6 kPa.

In the composite membrane module, i.e. the filter, the specific adsorption of the HIV virus or coronavirus is realized through the positively or negatively charged particles in the adsorption membrane, so that the HIV virus or coronavirus can be adsorbed by the adsorption membrane and retained in the filter, and the filtered plasma is returned to the patient.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.

FIG. 1 is a schematic structural view of a composite membrane module according to an embodiment of the present application;

FIG. 2 is a schematic structural diagram of a filter according to an embodiment of the present application;

fig. 3A to 3D are test results of the filter according to the present application.

Detailed Description

Reference will now be made in detail to embodiments of the present application, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative and are only for the purpose of explaining the present application and are not to be construed as limiting the present application.

The following disclosure provides many different embodiments or examples for implementing different features of the application. In order to simplify the disclosure of the present application, specific example components and arrangements are described below. Of course, they are merely examples and are not intended to limit the present application. Moreover, the present application may repeat reference numerals and/or letters in the various examples, such repetition is for the purpose of simplicity and clarity and does not in itself dictate a relationship between the various embodiments and/or configurations discussed. In addition, examples of various specific processes and materials are provided herein, but one of ordinary skill in the art may recognize applications of other processes and/or use of other materials.

Example 1 adsorption Membrane

In this embodiment, an adsorption membrane for adsorbing HIV virus or coronavirus in plasma is first provided. The material of the adsorption film is lignin mixed with a plurality of particles with positive charges or negative charges, namely, the particles with positive charges or negative charges are uniformly dispersed in the lignin, and the adsorption film with a porous structure is further formed.

In this embodiment, the plurality of positively or negatively charged microparticles are used to adsorb the HIV virus or coronavirus, thereby achieving specific adsorption of the HIV virus or coronavirus. The positively or negatively charged particles are particles with a surface that is positively or negatively charged, such as but not limited to silicon particles with a surface that is positively or negatively charged, such that the particles are 1+, 2+, 1-, or 2-.

In the embodiment, the thickness of the filtering membrane is 3-5 mm; the pore of the porous structure is 0.1-0.6 micron.

Example 2. composite Membrane Module 100

In this embodiment, as shown in FIG. 1, a composite membrane assembly 100 is provided for filtering HIV virus or coronavirus from plasma.

As shown in fig. 1, the composite membrane module 100 may include at least one layer of the adsorption membrane according to example 1 and at least one end filtration membrane.

In the present embodiment, as shown in fig. 1, the composite membrane module 100 includes four layers of the first adsorption membrane 110, one layer of the second adsorption membrane 120, and one layer of the end filtration membrane 130, which are sequentially stacked. The first adsorption membrane 110 is the adsorption membrane described in example 1, and has a pore size of 0.6 μm. The second adsorption film 120 is also the adsorption film described in embodiment 1, and has a pore size of 0.1 to 0.3 μm. In the present embodiment, the bacteria in the plasma can permeate the first adsorption film 110 but cannot pass through the second adsorption film 120, and the HIV virus or coronavirus is adsorbed by the first adsorption film 110 and the second adsorption film 120.

In this embodiment, the terminal filtering membrane 130 has a porous structure, a pore size of 0.1 to 0.3 μm, and the material of the terminal filtering membrane 130 is polyethersulfone. The final filtering membrane 130 is used for intercepting and separating bacteria and particles thereof in the plasma by means of physical barrier.

EXAMPLE 3 Filter 200

In this embodiment, as shown in fig. 2, the present application also provides a filter 200 for filtering HIV virus or coronavirus in plasma. The filter 200 includes a first housing 211 and a second housing 212, wherein the first housing 211 and the second housing 212 are mutually fastened to form an accommodating space, and a composite membrane assembly 100 is disposed in the accommodating space. The composite membrane assembly 100 may have the structure of the composite membrane assembly 100 described in example 2. Specifically, the composite membrane assembly 100 includes at least one first adsorption membrane 110, at least one second adsorption membrane 120, and a final filtration membrane 130, which are stacked in the plasma flow direction. In this embodiment, the composite film module 100 described in embodiment 2 will be described as an example. That is, in the filter 200 of the present embodiment, the first adsorption membrane 110, the second adsorption membrane 120, and the end filtration membrane 130 are all porous structures, the pore size of the first adsorption membrane 110 is 0.6 μm, the pore size of the second adsorption membrane 120 is 0.1 to 0.3 μm, and the pore size of the end filtration membrane 130 is 0.1 to 0.3 μm. The first adsorption membrane 110 and the second adsorption membrane 120 are made of a material in which a plurality of positively or negatively charged fine particles are mixed with lignin, and the end filtration membrane 130 is made of polyethersulfone.

In this embodiment, the composite membrane module 100 includes four layers of the first adsorption membrane 110, one layer of the second adsorption membrane 120, and one layer of the end filtration membrane 130, which are sequentially stacked, as in example 2.

As shown in fig. 2, the first housing 211 is provided with a plasma inlet 201 and an exhaust port 202, so that plasma can smoothly flow in. As shown in fig. 2, the plasma inlet 201 is located at the center of the first housing 211, which facilitates the smooth and uniform plasma entering the composite membrane assembly 100 after entering the filter. The second housing 212 is provided with an outlet 203 for filtered plasma to flow out. Thus, the pressure difference between the first adsorption membrane 110 and the final filtration membrane 130 in the direction along the plasma flow direction (downward in the figure) is set to 0.3 to 0.6 kPa.

It will be understood by those skilled in the art that the first and

second housings

211 and 212 may be made of polysulfone or other injection molded materials.

When the composite membrane component is used, blood in a patient body is subjected to separation treatment of blood cells and plasma, the plasma with certain pressure enters the surface layer (namely, the first adsorption layer) of the composite membrane component of the filter 200 from the plasma inlet 201 at the top, and the exhaust port 202 can ensure the smooth inflow of the plasma. It will be understood by those skilled in the art that the first housing 211 and the second housing 212 are sealed by a silicone rubber gasket for suction filtration purposes. The pressure difference between the first adsorption membrane 110 and the terminal filtering membrane 130 is 0.3-0.6 kPa, so that the plasma directly leaks downwards through the composite membrane 100 after flowing in through the plasma inlet 201.

Verification examples

The filter of the present application is sent to south hospital of Zhongshan hospital affiliated to the university of double denier at the public health clinical center of Shanghai, and the results of the test report obtained by the applicant are shown in fig. 3A to 3C, and fig. 3D is a test report of control group serum.

In this verification example, after the control sera (designated as sample No. 1, sample No. 2, and sample No. 3, respectively) were filtered with the filters described in the application described in example 2, the virus content (copies/ml) of HIV was measured, and the measurement results shown in fig. 3A to 3D and table 1 were obtained.

TABLE 1 viral content of HIV in filtered sera

Group of	Viral content of HIV (copies/ml)
		Sample No. 1	775.00
Sample No. 2	687.00
		Sample No. 3	186.00
Control group	↑1.01E+05

As shown in fig. 3A to 3D and table 1, the filter of the present application has a specific adsorption filtration effect on HIV virus, and the removal rate reaches 99.2%.

The present application has been described in relation to the above embodiments, which are only examples for implementing the present application. It must be noted that the disclosed embodiments do not limit the scope of the application. Rather, modifications and equivalent arrangements included within the spirit and scope of the claims are included within the scope of the present application.

Claims

1. An adsorption film for adsorbing HIV virus or coronavirus, wherein the adsorption film is made of lignin mixed with a plurality of positively or negatively charged particles for adsorbing HIV virus or coronavirus; and, the adsorption film is a porous structure.

2. The adsorption film according to claim 1, wherein the thickness of the adsorption film is 3 to 5 mm.

3. An adsorption film according to claim 1, wherein the porous structure has a gap of 0.1 to 0.6 μm.

4. A composite membrane module for filtering HIV or coronavirus in plasma, comprising at least one adsorption membrane according to claim 1 and at least one end filtration membrane.

5. The composite membrane module of claim 4, wherein the composite membrane module comprises at least one first adsorbent membrane and at least one second adsorbent membrane; wherein the pore diameter of the first adsorption membrane is 0.6 micron, and the pore diameter of the second adsorption membrane is 0.1-0.3 micron.

6. The composite membrane module of claim 5, wherein the composite membrane module comprises four layers of the first adsorption membrane, the second adsorption membrane, and the end filtration membrane, which are stacked.

7. The composite membrane module according to claim 4, wherein the terminal filtering membrane has a porous structure and a pore size of 0.1-0.3 μm, and the material of the terminal filtering membrane is polyethersulfone.

8. A filter is used for filtering HIV virus or coronavirus in blood plasma, and is characterized by comprising a first shell and a second shell, wherein the first shell and the second shell are mutually clamped to form an accommodating space, and a composite membrane component is arranged in the accommodating space; the composite membrane assembly comprises at least one first adsorption membrane, at least one second adsorption membrane and a terminal filtering membrane which are laminated; wherein the content of the first and second substances,

the composite membrane assembly is formed along the plasma flow direction according to the arrangement mode of the first adsorption membrane, the second adsorption membrane and the terminal filtering membrane; and wherein the one or more of the one,

the first adsorption membrane, the second adsorption membrane and the terminal filtering membrane are all of a porous structure, the aperture of the first adsorption membrane is 0.6 micrometer, the aperture of the second adsorption membrane is 0.1-0.3 micrometer, the aperture of the terminal filtering membrane is 0.1-0.3 micrometer, the first adsorption membrane and the second adsorption membrane are made of materials mixed with a plurality of lignin with positive charge or negative charge particles, and the terminal filtering membrane is made of polyether sulfone.

9. The filter according to claim 8, wherein the pressure difference between the first adsorption membrane and the terminal filtration membrane in the plasma flow direction is 0.3 to 0.6 kPa.