CN112014429B - Cell membrane vibration detection method based on ultramicro electroosmotic flow regulation and control - Google Patents
Cell membrane vibration detection method based on ultramicro electroosmotic flow regulation and control Download PDFInfo
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- 210000000170 cell membrane Anatomy 0.000 title claims abstract description 49
- 238000005370 electroosmosis Methods 0.000 title claims abstract description 24
- 230000033228 biological regulation Effects 0.000 title claims abstract description 15
- 238000001514 detection method Methods 0.000 title claims abstract description 6
- 239000002071 nanotube Substances 0.000 claims abstract description 48
- 239000010453 quartz Substances 0.000 claims abstract description 42
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 42
- 210000004027 cell Anatomy 0.000 claims abstract description 40
- 238000000034 method Methods 0.000 claims abstract description 15
- 238000012544 monitoring process Methods 0.000 claims abstract description 8
- 229910021607 Silver chloride Inorganic materials 0.000 claims description 6
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 claims description 6
- 238000004113 cell culture Methods 0.000 claims description 5
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims description 2
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 2
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 2
- 239000012528 membrane Substances 0.000 abstract description 4
- 230000007547 defect Effects 0.000 abstract description 3
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- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
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- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
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- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
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- 206010008342 Cervix carcinoma Diseases 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 239000000232 Lipid Bilayer Substances 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
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- 238000013459 approach Methods 0.000 description 1
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- 238000005119 centrifugation Methods 0.000 description 1
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- 238000002474 experimental method Methods 0.000 description 1
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- 230000003834 intracellular effect Effects 0.000 description 1
- 238000004093 laser heating Methods 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000034515 regulation of cell shape Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
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Abstract
The invention provides a cell membrane vibration detection method based on ultramicro electroosmotic flow regulation, which is characterized in that different cells and different states of the same type of cells are distinguished through cell membrane vibration frequency caused by the electroosmotic flow regulation in a quartz nanotube, and the tip size of the quartz nanotube is 50-300 nm. The invention overcomes the defects of time consumption, difficult operation and the like of the traditional cell membrane monitoring, the quartz nanotube is directly used for monitoring from a specific position on the surface of a single living cell membrane, the voltage applied to the electrode end can accurately control electroosmosis flow generated by the tip of the quartz nanotube, and the electroosmosis flow can drive the cell membrane to vibrate, so that the cell membrane biophysics of the single cell level can be more comprehensively understood. The method provides a simple and reliable method for simultaneously analyzing the local and global membrane vibration of single living cells, has small damage and provides a tool for quantifying drugs, diseases or cell structural mutation.
Description
Technical Field
The invention relates to a cell imaging technology, in particular to a method for detecting cell membrane vibration based on an ultramicro electroosmotic flow regulation technology.
Background
The cell membrane, also called plasma membrane, is a very thin membrane that surrounds the cell surface and is mainly composed of phospholipids and membrane proteins. The basic function of the cell membrane is to maintain the relative stability of the intracellular microenvironment and to participate in substance exchange, energy and information transfer with the external environment. In addition, it plays an important role in cell survival, growth, division, and differentiation. Cell membranes are not only cell boundaries, but also expression platforms for many proteins and glycans, which are used to regulate a variety of cell essential functions such as cellular communication, metabolism and transport. The cell membrane is composed of lipid bilayer and can vibrate and deform, which is critical for the regulation of cell shape. Membrane vibration plays an important role in cell behavior and is a key factor reflecting the physiological state of cells. However, previous studies of cell mechanical properties often require complex equipment or require indirect studies through exogenous modification.
Traditional patch clamp measurement method: when the opening quartz electrode touches the cell membrane, the opening end and the cell membrane form a very small sealing area together, so that the sealing area is isolated from the surrounding environment, the ion current of the area is monitored, and the traditional cell membrane has the defects of time consumption, difficult operation and the like.
Disclosure of Invention
The invention aims to provide a cell membrane vibration detection method based on ultramicro electroosmotic flow regulation, which does not need to use any external mark and only uses quartz nanotubes to accurately measure the vibration of a cell membrane.
A second object of the present invention is to provide the use of quartz nanotubes in monitoring cell membranes.
In order to achieve the first object, the present invention provides a method for detecting cell membrane vibration based on ultramicro electroosmotic flow regulation, which is characterized in that different cells and different states of the same cell are distinguished by cell membrane vibration frequency caused by electroosmotic flow regulation in a quartz nanotube, and the tip size of the quartz nanotube is 50nm-300nm.
As a preferred embodiment, the in-detect circuit is formed by: the silver wire is soaked in ferric chloride solution overnight, one Ag/AgCl electrode is soaked in the solution inside the quartz nano tube, and the other Ag/AgCl electrode is soaked in the cell culture solution.
As a preferable scheme, the quartz nano tube is drawn by a laser drawing instrument P-2000, and the instrument parameters are specifically set as follows: line 1:Heat 650,Fil 3,Vel 35,Del 145,Pul 75; line 2:Heat 920,Fil 2,Vel 15,Del 128,Pul 200.
As a preferred embodiment, the tip size of the quartz nanotube is 100nm.
In order to achieve the second object, the present invention provides an application of a quartz nanotube in monitoring cell membranes, wherein different cells and different states of the same cell are distinguished by cell membrane vibration frequency caused by electroosmotic flow regulation in the quartz nanotube, and the tip size of the quartz nanotube is 50nm-300nm.
The invention selects two kinds of cells: human breast cancer cells (MCF-7) and cervical cancer cells (HeLa) were cultured on the wall overnight. The cell membranes of MCF-7 cells and HeLa cells are accurately measured by using the nanotubes, and voltage is regulated at two ends of the electrode to cause the cell membranes to vibrate.
The invention has the advantages that the invention discloses a method for detecting the vibration frequency of a cell membrane based on a quartz nano pore canal, which does not need to use any external mark, overcomes the defects of time consumption, difficult operation and the like of the traditional cell membrane monitoring, directly monitors from a specific position on the surface of a single living cell membrane through a quartz nano tube, can accurately control electroosmosis flow generated by the tip of the quartz nano tube through voltage applied to an electrode end, can drive the cell membrane to vibrate, and has more comprehensive understanding on the cell membrane biophysics of the single cell level. The method provides a simple and reliable method for simultaneously analyzing the local and global membrane vibration of single living cells, has small damage and provides a tool for quantifying drugs, diseases or cell structural mutation.
Drawings
FIG. 1.A schematic diagram of a nanotube device, b vibration of cell membrane caused by electroosmotic flow at the tip of the nanotube, c continuous current plot of the nanotube at 0 mV.
Fig. 2. According to the current signal, the time domain of the current can be decomposed into two different frequencies: local vibrations under the action of electroosmotic flow and the natural frequency of the cell membrane itself.
Detailed Description
Hereinafter, the technology of the present invention will be described in detail with reference to the specific embodiments. It should be understood that the following detailed description is merely intended to aid those skilled in the art in understanding the invention, and is not intended to limit the invention.
Example 1.
(1) The quartz nano tube is prepared by laser heating a quartz capillary tube through a P-2000 laser drawing instrument. The specific method comprises the following steps: the quartz capillary tubes were sequentially cleaned in ethanol, acetone and pure water, respectively, for about 30 minutes, and finally blow-dried with nitrogen. Setting parameters of a laser drawing instrument program to obtain quartz nanometer pore channels with different sizes. First, the capillary was placed on P-2000, placed symmetrically, and drawn after the carbon dioxide laser was stabilized. Different parameters in the procedure affect the pore size of the quartz nanotubes and the length of the tube tip drawn. The parameters to be set are heating temperature (HEAT): 0-999, which is a quartz capillary tube used for heating and melting; FILAMENT (FILAMENT): 0-15, which are used for setting different scanning speeds of laser; speed (VELOCITY): 0-255, which is to adjust the moving speed of the pull rod; DELAY (DELAY) affects the length of the tip of the quartz nanotube, when the DELAY is 128 values, the laser irradiation is finished to start the pull rod, the DELAY is more than 128 values, the pull rod starts to move after a period of time is required to elapse after the laser is finished, the DELAY is less than 128 values, and the pull rod starts to move when the laser is not finished; tension (PULL): 0-255, for adjusting the pulling force applied to the quartz capillary, the magnitude of the pulling force also affects the diameter and length of the nanotube tip. In the invention, a two-step method is used for drawing the nanotube, and the parameters are as follows:
Line 1:Heat 650,Fil 3,Vel 35,Del 145,Pul 75；
Line 2:Heat 920,Fil 2,Vel 15,Del 128,Pul 200。
(2) Cells are cultured using conventional methods. MCF-7 cell culture: RPMI 1640, fetal bovine serum (10%), streptomycin (100. Mu.g mL -1 ) And penicillin (100. Mu.g mL) -1 ) Preparing a culture solution. HeLa cell culture: DMEM, fetal bovine serum (10%), streptomycin (100 μg mL) -1 ) And penicillin (100. Mu.g mL) -1 ) Preparing a culture solution. Culture flask and cultureThe cells in the dishes were all cultured at 37℃under 5% carbon dioxide.
(3) 2mM PBS solution was injected into the quartz nanotubes using a micro-syringe, and the remaining air in the nanotubes was removed by centrifugation to ensure that the solution reached the tip of the tube. One Ag/AgCl electrode is immersed in the solution inside the quartz nanotube, and the other Ag/AgCl electrode is immersed in the cell culture solution. The experimental method comprises the following steps: the sample stage is temporarily not placed with cells, the nanotube tip is moved to the middle of the visual field through movement in X, Y and Z axis directions, then the XY axis is not moved, the nanotube is lifted up only by moving the Z axis, after the nanotube is lifted to a sufficient height, the experimental cells are placed on the sample stage, single MCF-7 cells (or HeLa cells) with good morphology are found, the cells are moved to the visual field, at the moment, the sample stage is kept still, and the nanotube is lowered again until the nanotube is lowered into the culture solution but the state of the cells is not touched yet. The tube and the cell are then gradually focused clearly until they approach the same plane, at which time the micro-operation with less torque is exchanged, the current software is turned on to start recording, and when the current fluctuates instantaneously, it is proved that the nanotube has touched the cell membrane.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (5)
1.A cell membrane vibration detection method based on ultramicro electroosmotic flow regulation is characterized in that different cells and different states of the same type of cells are distinguished through cell membrane vibration frequency caused by electroosmotic flow regulation in a quartz nanotube, the tip size of the quartz nanotube is 50nm-300nm,
the method comprises the steps of monitoring a specific position on the surface of a single living cell membrane through a quartz nanotube, accurately controlling electroosmosis flow generated by the tip of the quartz nanotube by direct-current voltage applied to an electrode end, driving the cell membrane of the single cell to vibrate by the electroosmosis flow, and obtaining a track of continuous current of the quartz nanotube to time; from the current signal, the time domain of the current is decomposed into two different frequencies: the high frequency at which the cell membrane vibrates locally and the natural frequency of the cell membrane itself.
2. The method for detecting the vibration of the cell membrane based on the ultra-micro electroosmotic flow regulation according to claim 1, wherein the circuit in detection is formed by the following steps: the silver wire is soaked in ferric chloride solution overnight, one Ag/AgCl electrode is soaked in the solution inside the quartz nano tube, and the other Ag/AgCl electrode is soaked in the cell culture solution.
3. The method for detecting the vibration of the cell membrane based on the ultra-micro electroosmotic flow regulation and control according to claim 1, wherein the quartz nanotube is drawn by a laser drawing instrument P-2000, and the instrument parameters are specifically set as follows: line 1:Heat 650,Fil 3,Vel 35,Del 145,Pul 75; line 2:Heat 920,Fil 2,Vel 15,Del 128,Pul 200.
4. The method for detecting the vibration of the cell membrane based on the ultra-micro electroosmotic flow regulation according to claim 1, wherein the tip size of the quartz nanotube is 100nm.
5. The application of the quartz nano tube in monitoring cell membranes is characterized in that different cells and different states of the same type of cells are distinguished by the cell membrane vibration frequency caused by the electroosmotic flow regulation in the quartz nano tube, the tip size of the quartz nano tube is 50nm-300nm,
the method comprises the steps of monitoring a specific position on the surface of a single living cell membrane through a quartz nanotube, accurately controlling electroosmosis flow generated by the tip of the quartz nanotube by direct-current voltage applied to an electrode end, driving the cell membrane of the single cell to vibrate by the electroosmosis flow, and obtaining a track of continuous current of the quartz nanotube to time; from the current signal, the time domain of the current is decomposed into two different frequencies: the high frequency at which the cell membrane vibrates locally and the natural frequency of the cell membrane itself.
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07159698A (en) * | 1993-12-07 | 1995-06-23 | Olympus Optical Co Ltd | Micromanipulator and driving method of micromanipulator |
| US7384733B1 (en) * | 1998-12-05 | 2008-06-10 | Xention Discovery Limited | Interface patch clamping |
| WO2008092607A1 (en) * | 2007-01-31 | 2008-08-07 | Universität Wien | Pipette device, manipulation device and method for manipulating biological cells |
| DE102008024803A1 (en) * | 2007-05-23 | 2008-11-27 | Grönemeyer, Dietrich H.W., Prof. Dr. | Device for determining a resonant frequencies of a cell sample e.g. tumor cells for diagnostic purposes, comprises a holding device, a first frequency generator, an observation mechanism, a bringing device, and a second frequency generator |
| CN102071135A (en) * | 2009-11-20 | 2011-05-25 | 国家纳米技术与工程研究院 | High resolution patch clamp based on scanning probe microscopy technology and operating method thereof |
| JP2011147400A (en) * | 2010-01-22 | 2011-08-04 | Hamamatsu Univ School Of Medicine | Cell discrimination method, reference data formation method for cell discrimination, and cell distinguishing device |
| CN102353818A (en) * | 2011-06-23 | 2012-02-15 | 国家纳米技术与工程研究院 | Device and method for evaluating neuron-like differentiation degree of PC12 cell |
| CN102455355A (en) * | 2010-10-22 | 2012-05-16 | 国家纳米技术与工程研究院 | Apparatus and method for rapidly assessing nano-material on biological security of breathing system |
| CN102636551A (en) * | 2012-04-18 | 2012-08-15 | 南京师范大学 | Dynamic detection method of potassium ion exchange inside and outside HEK (human embryonic kidney) 293 cell and erythrocyte |
| CN107407657A (en) * | 2015-02-25 | 2017-11-28 | 加利福尼亚大学董事会 | Individual cells intracellular nanometer PH probes |
| CN107907588A (en) * | 2017-12-15 | 2018-04-13 | 湖南农业大学 | A kind of cyto-mechanics characteristic tester based on STM32 |
| CN110514634A (en) * | 2019-09-02 | 2019-11-29 | 华东理工大学 | Unicellular glycosyl metabolism labeling method based on glass nano electrode |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8232074B2 (en) * | 2002-10-16 | 2012-07-31 | Cellectricon Ab | Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells |
-
2019
- 2019-05-30 CN CN201910461520.5A patent/CN112014429B/en active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07159698A (en) * | 1993-12-07 | 1995-06-23 | Olympus Optical Co Ltd | Micromanipulator and driving method of micromanipulator |
| US7384733B1 (en) * | 1998-12-05 | 2008-06-10 | Xention Discovery Limited | Interface patch clamping |
| WO2008092607A1 (en) * | 2007-01-31 | 2008-08-07 | Universität Wien | Pipette device, manipulation device and method for manipulating biological cells |
| DE102008024803A1 (en) * | 2007-05-23 | 2008-11-27 | Grönemeyer, Dietrich H.W., Prof. Dr. | Device for determining a resonant frequencies of a cell sample e.g. tumor cells for diagnostic purposes, comprises a holding device, a first frequency generator, an observation mechanism, a bringing device, and a second frequency generator |
| CN102071135A (en) * | 2009-11-20 | 2011-05-25 | 国家纳米技术与工程研究院 | High resolution patch clamp based on scanning probe microscopy technology and operating method thereof |
| JP2011147400A (en) * | 2010-01-22 | 2011-08-04 | Hamamatsu Univ School Of Medicine | Cell discrimination method, reference data formation method for cell discrimination, and cell distinguishing device |
| CN102455355A (en) * | 2010-10-22 | 2012-05-16 | 国家纳米技术与工程研究院 | Apparatus and method for rapidly assessing nano-material on biological security of breathing system |
| CN102353818A (en) * | 2011-06-23 | 2012-02-15 | 国家纳米技术与工程研究院 | Device and method for evaluating neuron-like differentiation degree of PC12 cell |
| CN102636551A (en) * | 2012-04-18 | 2012-08-15 | 南京师范大学 | Dynamic detection method of potassium ion exchange inside and outside HEK (human embryonic kidney) 293 cell and erythrocyte |
| CN107407657A (en) * | 2015-02-25 | 2017-11-28 | 加利福尼亚大学董事会 | Individual cells intracellular nanometer PH probes |
| CN107907588A (en) * | 2017-12-15 | 2018-04-13 | 湖南农业大学 | A kind of cyto-mechanics characteristic tester based on STM32 |
| CN110514634A (en) * | 2019-09-02 | 2019-11-29 | 华东理工大学 | Unicellular glycosyl metabolism labeling method based on glass nano electrode |
Non-Patent Citations (5)
| Title |
|---|
| Low-coherent quantitative phase microscope for nanometer-scale measurement of living cells morphology;Toyohiko Yamauchi, et al.;OPTICS EXPRESS;第16卷(第16期);12227-12238 * |
| Probing the Membrane Vibration of Single Living Cells by Using Nanopipettes;Bin-Bin Chen, et al.;CHEMBIOCHEM;第21卷(第5期);650-655 * |
| 基于扫描离子电导显微镜负反馈扫描控制技术的高分辨率膜片钳技术;杨茜;刘晓;张晓帆;卢虎杰;张彦军;;生理学报(03);275-283 * |
| 扫描离子电导显微镜在生物学研究的应用;张娜;郑金华;宋武琦;乔远东;金连弘;牛木正男;张凤民;;中国医学装备(第S2期);215-216 * |
| 扫描离子电导显微镜在细胞表征中的应用研究;郎瑾新，等;中国科学:化学;第49卷(第6期);844-860 * |
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