CN112010712A - Strawberry color value repairing fertilizer and preparation method thereof - Google Patents

Strawberry color value repairing fertilizer and preparation method thereof Download PDF

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Publication number
CN112010712A
CN112010712A CN202010755408.5A CN202010755408A CN112010712A CN 112010712 A CN112010712 A CN 112010712A CN 202010755408 A CN202010755408 A CN 202010755408A CN 112010712 A CN112010712 A CN 112010712A
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parts
fertilizer
weight
amino acid
strawberry
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张力
万建华
徐化林
杨世立
田文成
郭卫红
汪波
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Huaqiang Chemical Group Co Ltd
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Huaqiang Chemical Group Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02—Superphosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/10—Solid or semi-solid fertilisers, e.g. powders
    • C05G5/12—Granules or flakes
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/30—Layered or coated, e.g. dust-preventing coatings

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Fertilizers (AREA)

Abstract

The invention provides a strawberry color value repairing fertilizer which comprises the following components in parts by weight: 90-110 parts of ammonium sulfate, 22-42 parts of triple superphosphate, 263 parts of urea 243-. The strawberry cultivation method has the advantages that the uniform growth of strawberry crops is promoted, the morbidity of the strawberries is reduced, the planted strawberries cannot be deformed, the finally obtained strawberries are more nutritious, the taste is better, the appearance color value is higher, and the strawberries are more easily accepted by markets.

Description

Strawberry color value repairing fertilizer and preparation method thereof
Technical Field
The invention relates to the technical field of fertilizers, and particularly relates to a strawberry color value repairing fertilizer and a preparation method thereof.
Background
The strawberry is sweet in taste, bright red in color and small in appearance, and is popular with female consumers. At present, more than 2000 varieties of strawberries exist in the world, in order to meet the domestic market, various strawberries are introduced from Japan and other countries in China, and because the characteristics of the various strawberries such as color, taste, appearance, size, growth period, growth environment requirements, disease and insect resistance and the like are different, a certain gap exists between the planting output and the market demand. A batch of new strawberry varieties are cultured by various agricultural scientific research institutions in China through technologies such as grafting, hybridization and the like, great breakthrough is made in yield, color, taste and appearance, but new problems also occur, for example, the marketing of strawberries in the market is influenced because the probability of hollowness and deformity of the strawberries is high. The reason for this is that the plants of strawberries are very dependent on the fertility of soil, and if the effect of nutrient absorption of the root systems of strawberries is not good due to the soil environment, the strawberries are hollow and grow abnormally.
Disclosure of Invention
The invention aims to provide a strawberry color value repairing fertilizer, which solves the problems of hollowness and appearance deformity of strawberries after the conventional fertilizer is applied, and also provides a preparation method of the strawberry color value repairing fertilizer.
In order to solve the technical problem, the invention provides the following scheme: the strawberry color value repairing fertilizer comprises the following components in parts by weight: 90-110 parts of ammonium sulfate, 22-42 parts of triple superphosphate, 263 parts of urea 243-.
Furthermore, by weight, 90-110 parts of ammonium sulfate, 22-42 parts of triple superphosphate, 263 parts of urea 243-.
Further, the paint comprises the following components in parts by weight: 100 parts of ammonium sulfate, 32 parts of triple superphosphate, 253 parts of urea, 200 parts of superphosphate, 20 parts of calcium magnesium phosphate fertilizer, 320 parts of potassium sulfate, 10 parts of borax, 18 parts of trace element package, 10 parts of polyamine peptide and 0.5 part of amino acid-containing water-soluble fertilizer, wherein the nutrient content of the strawberry color value restoration fertilizer is 35%.
Further, the amino acid-containing water-soluble fertilizer is a mixture of cyclic dipeptide, pyrimidine nucleoside, amino acid and sugar.
Further, the polyamine peptide is a mixture of polyglutamic acid, active amino acids, organic matters and crude plant proteins.
Further, the trace element package is a mixture of zinc chelate, manganese chelate, boric acid and ammonium heptamolybdate.
Furthermore, in the trace element package, according to the weight proportion, the weight proportion of the chelated zinc is 40-45%, the weight proportion of the chelated manganese is 23-28%, the weight proportion of the boric acid is 29-33%, and the weight proportion of the ammonium heptamolybdate is 1-2%.
The invention also provides a preparation method of the strawberry color value restoration fertilizer, which comprises the following steps:
s1, preparing 90-110 parts of ammonium sulfate, 22-42 parts of triple superphosphate, 263 parts of urea 243-;
s2: granulating the mixed product in the S1 to prepare a coarse fertilizer;
s3, coating 8-12 parts of polyamine peptide and 0.4-0.6 part of amino acid-containing water-soluble fertilizer in the crude fertilizer in the S2 to form a finished product.
Further, the method comprises the following steps:
s1, preparing 90-110 parts of ammonium sulfate, 22-42 parts of triple superphosphate, 263 parts of urea 243-;
s2: granulating the mixed product in the S1 to prepare a coarse fertilizer;
s3, coating 8-28 parts of trace element package, 8-12 parts of polyamine peptide and 0.4-0.6 part of amino acid-containing water-soluble fertilizer in the crude fertilizer in the S2 to form a finished product, wherein the nutrient content of the finished product is 30%.
Further, the step S1 includes:
s10: preparing 90-110 parts of ammonium sulfate, 22-42 parts of triple superphosphate, 190-210 parts of calcium superphosphate, 10-30 parts of calcium magnesium phosphate fertilizer, 330 parts of potassium sulfate and 310-12 parts of borax by weight part, and mixing;
s11: preparing 243 and 263 parts by weight of urea;
s12: mixing urea in S11 with the product obtained in S10;
the sequence of S10 and S11 is not fixed, and can be performed simultaneously or sequentially.
According to the invention, by utilizing the reasonable proportion and the cooperation of the components, the soil acid-base environment can be improved after the strawberry cultivation substrate is used, the effect of promoting the root growth of strawberry crops is achieved, the main roots and hairy roots of strawberries are enabled to be more, the nutrient absorption of the crops is improved, the hollow phenomenon of the strawberries is avoided, the soil environment can be effectively repaired, and the growth deformity of the strawberries is avoided.
Compared with the prior art, the strawberry cultivation method has the advantages that the uniform growth of strawberry crops is promoted, the morbidity of strawberries is reduced, and the planted strawberries are not deformed, so that the finally obtained strawberries are more nutritious, have better mouthfeel and higher appearance color value, and are more easily accepted by markets.
Detailed Description
The present invention is further described below in conjunction with specific examples so that those skilled in the art may better understand the present invention and practice it. The following discloses many different embodiments or examples for implementing the subject technology described. Specific examples of one or more arrangements of features presented below are set forth to simplify the disclosure, but are not intended to limit the invention.
Example one
The strawberry color value repairing fertilizer comprises the following components in parts by weight: 90 parts of ammonium sulfate, 22 parts of triple superphosphate, 243 parts of urea, 190 parts of superphosphate, 10 parts of calcium magnesium phosphate fertilizer, 310 parts of potassium sulfate, 8 parts of borax, 8 parts of polyamine peptide and 0.4 part of amino acid-containing water-soluble fertilizer.
The amino acid-containing water-soluble fertilizer is a mixture of cyclic dipeptide, pyrimidine nucleoside, amino acid and sugar, and the weight ratio of cyclic dipeptide: pyrimidine nucleosides: amino acids: the sugars were mixed 1:1:1: 1.
The polyamine peptide is a mixture of polyglutamic acid, active amino acid, organic matters and crude plant protein, wherein the weight ratio of the polyglutamic acid: active amino acids: organic matter: crude protein was mixed 2.5:8:20: 60.
The preparation method comprises the following steps:
s1, weighing 90 kg of ammonium sulfate, 22 kg of heavy calcium superphosphate, 190 kg of calcium superphosphate, 10 kg of calcium magnesium phosphate fertilizer, 310 kg of potassium sulfate and 8 kg of borax by a material proportioning and weighing scale, and conveying the weighed materials to a mixing tank;
s2, weighing 243 kg of molten urea from the urea process step through proportioning and weighing, and conveying the weighed molten urea to a mixing tank; the sequence of S1 and S2 is not fixed, and can be carried out simultaneously or sequentially, so that the mixing result is not influenced;
mixing the raw materials in S3, S1 and S2 in a mixing tank, and then conveying the mixture to a granulator through a potential difference, wherein the mixture flows out from the bottom of the mixing tank;
s4, spraying the mixture in the step S3 from the top of the granulating tower by a granulator through a rotating centrifugal force to form a coarse fertilizer, and feeding the coarse fertilizer into a cooling system;
s5, preparing cyclic dipeptide according to the mass ratio: pyrimidine nucleosides: amino acids: mixing the sugar in a ratio of 1:1:1:1 to obtain the amino acid-containing water-soluble fertilizer; the weight ratio of polyglutamic acid: active amino acids: organic matter: mixing crude protein 2.5:8:20:60 to obtain polyamine peptide;
s6, cooling the coarse fertilizer by air cooling of an empty tower of a cooling system, screening out coarse fertilizer particles with qualified particle sizes, sending the coarse fertilizer particles to a coating machine, weighing 8 kg of polyamine peptide and 0.4 kg of amino acid-containing water-soluble fertilizer by a batching scale, mixing the polyamine peptide, the amino acid-containing water-soluble fertilizer and liquid coating oil, adding the mixture into the coating machine, wrapping the polyamine peptide and the amino acid-containing water-soluble fertilizer outside the coarse fertilizer particles to form a finished product of strawberry color value repairing fertilizer, and sending the finished product of strawberry color value repairing fertilizer to a finished product package.
Example two
The strawberry color value repairing fertilizer comprises the following components in parts by weight: 110 parts of ammonium sulfate, 42 parts of triple superphosphate, 263 parts of urea, 210 parts of superphosphate, 30 parts of calcium magnesium phosphate fertilizer, 330 parts of potassium sulfate, 12 parts of borax, 12 parts of polyamine peptide and 0.6 part of amino acid-containing water-soluble fertilizer.
The amino acid-containing water-soluble fertilizer is a mixture of cyclic dipeptide, pyrimidine nucleoside, amino acid and sugar, and the weight ratio of cyclic dipeptide: pyrimidine nucleosides: amino acids: the sugars were mixed 1:1:1: 1.
The polyamine peptide is a mixture of polyglutamic acid, active amino acid, organic matters and crude plant protein, wherein the weight ratio of the polyglutamic acid: active amino acids: organic matter: crude protein was mixed 2.5:8:20: 60.
The preparation method comprises the following steps:
s1, weighing 110 kg of ammonium sulfate, 42 kg of heavy calcium superphosphate, 210 kg of calcium superphosphate, 30 kg of calcium magnesium phosphate fertilizer, 330 kg of potassium sulfate and 12 kg of borax by material proportioning and weighing, and conveying to a mixing tank;
s2, 263 kilograms of molten urea sent from the urea process is weighed by the batching scale and then is conveyed to a mixing tank; the sequence of S1 and S2 is not fixed, and can be carried out simultaneously or sequentially, so that the mixing result is not influenced;
mixing the raw materials in S3, S1 and S2 in a mixing tank, and then conveying the mixture to a granulator through a potential difference, wherein the mixture flows out from the bottom of the mixing tank;
s4, spraying the mixture in the step S3 from the top of the granulating tower by a granulator through a rotating centrifugal force to form a coarse fertilizer, and feeding the coarse fertilizer into a cooling system;
s5, preparing cyclic dipeptide according to the mass ratio: pyrimidine nucleosides: amino acids: mixing the sugar in a ratio of 1:1:1:1 to obtain the amino acid-containing water-soluble fertilizer; the weight ratio of polyglutamic acid: active amino acids: organic matter: mixing crude protein 2.5:8:20:60 to obtain polyamine peptide;
s6, cooling the coarse fertilizer by air cooling of an empty tower of a cooling system, screening out coarse fertilizer particles with qualified particle sizes, sending the coarse fertilizer particles to a coating machine, weighing 12 kg of polyamine peptide and 0.6 kg of amino acid-containing water-soluble fertilizer by a batching scale, mixing the polyamine peptide, the amino acid-containing water-soluble fertilizer and liquid coating oil, adding the mixture into the coating machine, wrapping the polyamine peptide and the amino acid-containing water-soluble fertilizer outside the coarse fertilizer particles to form a finished product of strawberry color value repairing fertilizer, and sending the finished product of strawberry color value repairing fertilizer to a finished product package.
EXAMPLE III
The strawberry color value repairing fertilizer comprises the following components in parts by weight: 100 parts of ammonium sulfate, 32 parts of triple superphosphate, 253 parts of urea, 200 parts of superphosphate, 20 parts of calcium magnesium phosphate fertilizer, 320 parts of potassium sulfate, 10 parts of borax, 10 parts of polyamine peptide and 0.5 part of amino acid-containing water-soluble fertilizer.
The amino acid-containing water-soluble fertilizer is a mixture of cyclic dipeptide, pyrimidine nucleoside, amino acid and sugar, and the weight ratio of cyclic dipeptide: pyrimidine nucleosides: amino acids: the sugars were mixed 1:1:1: 1.
The polyamine peptide is a mixture of polyglutamic acid, active amino acid, organic matters and crude plant protein, wherein the weight ratio of the polyglutamic acid: active amino acids: organic matter: crude protein was mixed 2.5:8:20: 60.
The preparation method comprises the following steps:
s1, weighing 100 kg of ammonium sulfate, 32 kg of heavy superphosphate, 200 kg of superphosphate, 20 kg of calcium magnesium phosphate fertilizer, 320 kg of potassium sulfate and 10 kg of borax by material proportioning and weighing, and conveying to a mixing tank;
s2, weighing 253 kilograms of molten urea from the urea process and conveying the molten urea to a mixing tank; the sequence of S1 and S2 is not fixed, and can be carried out simultaneously or sequentially, so that the mixing result is not influenced;
mixing the raw materials in S3, S1 and S2 in a mixing tank, and then conveying the mixture to a granulator through a potential difference, wherein the mixture flows out from the bottom of the mixing tank;
s4, spraying the mixture in the step S3 from the top of the granulating tower by a granulator through a rotating centrifugal force to form a coarse fertilizer, and feeding the coarse fertilizer into a cooling system;
s5, preparing cyclic dipeptide according to the mass ratio: pyrimidine nucleosides: amino acids: mixing the sugar in a ratio of 1:1:1:1 to obtain the amino acid-containing water-soluble fertilizer; the weight ratio of polyglutamic acid: active amino acids: organic matter: mixing crude protein 2.5:8:20:60 to obtain polyamine peptide;
s6, cooling the coarse fertilizer by air cooling of an empty tower of a cooling system, screening out coarse fertilizer particles with qualified particle sizes, sending the coarse fertilizer particles to a coating machine, weighing 10 kg of polyamine peptide and 0.5 kg of amino acid-containing water-soluble fertilizer by a batching scale, mixing the polyamine peptide, the amino acid-containing water-soluble fertilizer and liquid coating oil, adding the mixture into the coating machine, wrapping the polyamine peptide and the amino acid-containing water-soluble fertilizer outside the coarse fertilizer particles to form a finished product of strawberry color value repairing fertilizer, and sending the finished product of strawberry color value repairing fertilizer to a finished product package.
Example four
The strawberry color value repairing fertilizer comprises the following components in parts by weight: 90 parts of ammonium sulfate, 22 parts of triple superphosphate, 243 parts of urea, 190 parts of superphosphate, 10 parts of calcium magnesium phosphate fertilizer, 310 parts of potassium sulfate, 8 parts of borax, 8 parts of trace element package, 8 parts of polyamine peptide and 0.4 part of amino acid-containing water-soluble fertilizer.
The amino acid-containing water-soluble fertilizer is a mixture of cyclic dipeptide, pyrimidine nucleoside, amino acid and sugar, and the weight ratio of cyclic dipeptide: pyrimidine nucleosides: amino acids: the sugars were mixed 1:1:1: 1.
The polyamine peptide is a mixture of polyglutamic acid, active amino acid, organic matters and crude plant protein, wherein the weight ratio of the polyglutamic acid: active amino acids: organic matter: crude protein was mixed 2.5:8:20: 60.
In the trace element package, the weight ratio of the chelated zinc is 40%, the weight ratio of the chelated manganese is 28%, the weight ratio of the boric acid is 30%, and the weight ratio of the ammonium heptamolybdate is 2%.
The nutrient content of the strawberry color value repairing fertilizer is 33%, and the nutrient content is N: p2O5：K2O ═ 13: 4: 16, compared to the conventional fertilizer N: p2O5：K2O15: 15: 15, the nutrient content is reduced, the cost is reduced, but after other raw materials are reasonably matched, the fertilizer is not affected to the growth of crops after being appliedAnd (6) sounding.
The preparation method comprises the following steps:
s1, weighing 90 kg of ammonium sulfate, 22 kg of heavy calcium superphosphate, 190 kg of calcium superphosphate, 10 kg of calcium magnesium phosphate fertilizer, 310 kg of potassium sulfate and 8 kg of borax by a material proportioning and weighing scale, and conveying the weighed materials to a mixing tank;
s2, weighing 243 kg of molten urea from the urea process step through proportioning and weighing, and conveying the weighed molten urea to a mixing tank; the sequence of S1 and S2 is not fixed, and can be carried out simultaneously or sequentially, so that the mixing result is not influenced;
mixing the raw materials in S3, S1 and S2 in a mixing tank, and then conveying the mixture to a granulator through a potential difference, wherein the mixture flows out from the bottom of the mixing tank;
s4, spraying the mixture in the step S3 from the top of the granulating tower by a granulator through a rotating centrifugal force to form a coarse fertilizer, and feeding the coarse fertilizer into a cooling system;
s5, preparing cyclic dipeptide according to the mass ratio: pyrimidine nucleosides: amino acids: mixing the sugar in a ratio of 1:1:1:1 to obtain the amino acid-containing water-soluble fertilizer; the weight ratio of polyglutamic acid: active amino acids: organic matter: mixing crude protein 2.5:8:20:60 to obtain polyamine peptide; chelating zinc according to the mass ratio: chelating manganese: boric acid: mixing heptamolybdic acid (40: 28:30: 2) to obtain a trace element package;
s6, cooling the coarse fertilizer by air cooling of an empty tower of a cooling system, screening out coarse fertilizer particles with qualified particle sizes, sending the coarse fertilizer particles to a coating machine, weighing 8 kg of polyamine peptide, 8 kg of a trace element package and 0.4 kg of an amino acid-containing water-soluble fertilizer by a batching scale, mixing the polyamine peptide, the trace element package, the amino acid-containing water-soluble fertilizer and liquid coating oil, adding the mixture into the coating machine, wrapping the polyamine peptide, the trace element package and the amino acid-containing water-soluble fertilizer outside the coarse fertilizer particles to form a finished product of the strawberry color value repairing fertilizer, and sending the finished product of the strawberry color value repairing fertilizer to a finished product package.
EXAMPLE five
The strawberry color value repairing fertilizer comprises the following components in parts by weight: 110 parts of ammonium sulfate, 42 parts of triple superphosphate, 263 parts of urea, 210 parts of superphosphate, 30 parts of calcium magnesium phosphate fertilizer, 330 parts of potassium sulfate, 12 parts of borax, 28 parts of trace element package, 12 parts of polyamine peptide and 0.6 part of amino acid-containing water-soluble fertilizer.
The amino acid-containing water-soluble fertilizer is a mixture of cyclic dipeptide, pyrimidine nucleoside, amino acid and sugar, and the weight ratio of cyclic dipeptide: pyrimidine nucleosides: amino acids: the sugars were mixed 1:1:1: 1.
The polyamine peptide is a mixture of polyglutamic acid, active amino acid, organic matters and crude plant protein, wherein the weight ratio of the polyglutamic acid: active amino acids: organic matter: crude protein was mixed at 5:10:30: 80.
In the trace element package, according to the weight ratio, the weight of the chelated zinc accounts for 45%, the weight of the chelated manganese accounts for 23%, the weight of the boric acid accounts for 31%, and the weight of the ammonium heptamolybdate accounts for 1%.
The nutrient content of the strawberry color value restoration fertilizer is 37%, and the nutrient content is N: p2O5：K2O15: 5: 17, compared to the conventional fertilizer N: p2O5：K2O15: 15: 15, the nutrient content is reduced, the cost is reduced, but after other raw materials are reasonably matched, the growth of crops is not affected after fertilization.
The preparation method comprises the following steps:
s1, weighing 110 kg of ammonium sulfate, 42 kg of heavy calcium superphosphate, 210 kg of calcium superphosphate, 30 kg of calcium magnesium phosphate fertilizer, 330 kg of potassium sulfate and 12 kg of borax by material proportioning and weighing, and conveying to a mixing tank;
s2, 263 kilograms of molten urea sent from the urea process is weighed by the batching scale and then is conveyed to a mixing tank; the sequence of S1 and S2 is not fixed, and can be carried out simultaneously or sequentially, so that the mixing result is not influenced;
mixing the raw materials in S3, S1 and S2 in a mixing tank, and then conveying the mixture to a granulator through a potential difference, wherein the mixture flows out from the bottom of the mixing tank;
s4, spraying the mixture in the step S3 from the top of the granulating tower by a granulator through a rotating centrifugal force to form a coarse fertilizer, and feeding the coarse fertilizer into a cooling system;
s5, preparing cyclic dipeptide according to the mass ratio: pyrimidine nucleosides: amino acids: mixing the sugar in a ratio of 1:1:1:1 to obtain the amino acid-containing water-soluble fertilizer; the weight ratio of polyglutamic acid: active amino acids: organic matter: mixing crude protein with the ratio of 5:10:30:80 to obtain polyamine peptide; chelating zinc according to the mass ratio: chelating manganese: boric acid: mixing heptamolybdic acid 45:23:31:1 to obtain a trace element package;
s6, cooling the coarse fertilizer by air cooling of an empty tower of a cooling system, screening out coarse fertilizer particles with qualified particle sizes, sending the coarse fertilizer particles to a coating machine, weighing 12 kg of polyamine peptide, 28 kg of trace element package and 0.6 kg of amino acid-containing water-soluble fertilizer by a batching scale, mixing the polyamine peptide, the trace element package, the amino acid-containing water-soluble fertilizer and liquid coating oil, adding the mixture into the coating machine, wrapping the polyamine peptide, the trace element package and the amino acid-containing water-soluble fertilizer outside the coarse fertilizer particles to form a finished product strawberry color value repairing fertilizer, and sending the finished product strawberry color value repairing fertilizer to a finished product package.
EXAMPLE six
The strawberry color value repairing fertilizer comprises the following components in parts by weight: 100 parts of ammonium sulfate, 32 parts of triple superphosphate, 253 parts of urea, 200 parts of superphosphate, 20 parts of calcium magnesium phosphate fertilizer, 320 parts of potassium sulfate, 10 parts of borax, 18 parts of trace element package, 10 parts of polyamine peptide and 0.5 part of amino acid-containing water-soluble fertilizer.
The amino acid-containing water-soluble fertilizer is a mixture of cyclic dipeptide, pyrimidine nucleoside, amino acid and sugar, and the weight ratio of cyclic dipeptide: pyrimidine nucleosides: amino acids: the sugars were mixed 1:1:1: 1.
The polyamine peptide is a mixture of polyglutamic acid, active amino acid, organic matters and crude plant protein, wherein the weight ratio of the polyglutamic acid: active amino acids: organic matter: crude protein was mixed 3:9:25: 70.
In the trace element package, according to the weight proportion, the weight of the chelated zinc accounts for 44%, the weight of the chelated manganese accounts for 25%, the weight of the boric acid accounts for 29%, and the weight of the ammonium heptamolybdate accounts for 2%.
The nutrient content of the strawberry color value restoration fertilizer is 35%, and the nutrient content is N: p2O5：K2O ═ 14: 5: 16, compared to the conventional fertilizer N: p2O5：K2O15: 15: 15, the nutrient content of the fertilizer is reduced,the cost is reduced, but after other raw materials are reasonably matched, the growth of crops is not affected after fertilization.
The preparation method comprises the following steps:
s1, weighing 100 kg of ammonium sulfate, 32 kg of heavy superphosphate, 200 kg of superphosphate, 20 kg of calcium magnesium phosphate fertilizer, 320 kg of potassium sulfate and 10 kg of borax by material proportioning and weighing, and conveying to a mixing tank;
s2, weighing 253 kilograms of molten urea from the urea process and conveying the molten urea to a mixing tank; the sequence of S1 and S2 is not fixed, and can be carried out simultaneously or sequentially, so that the mixing result is not influenced;
mixing the raw materials in S3, S1 and S2 in a mixing tank, and then conveying the mixture to a granulator through a potential difference, wherein the mixture flows out from the bottom of the mixing tank;
s4, spraying the mixture in the step S3 from the top of the granulating tower by a granulator through a rotating centrifugal force to form a coarse fertilizer, and feeding the coarse fertilizer into a cooling system;
s5, preparing cyclic dipeptide according to the mass ratio: pyrimidine nucleosides: amino acids: mixing the sugar in a ratio of 1:1:1:1 to obtain the amino acid-containing water-soluble fertilizer; the weight ratio of polyglutamic acid: active amino acids: organic matter: mixing crude protein 3:9:25:70 to obtain polyamine peptide; chelating zinc according to the mass ratio: chelating manganese: boric acid: mixing 44:25:29:2 heptamolybdic acid to obtain a trace element package;
s6, cooling the coarse fertilizer by air cooling of an empty tower of a cooling system, screening out coarse fertilizer particles with qualified particle sizes, sending the coarse fertilizer particles to a coating machine, weighing 10 kg of polyamine peptide, 18 kg of trace element package and 0.5 kg of amino acid-containing water-soluble fertilizer by a batching scale, mixing the polyamine peptide, the trace element package, the amino acid-containing water-soluble fertilizer and liquid coating oil, adding the mixture into the coating machine, wrapping the polyamine peptide, the trace element package and the amino acid-containing water-soluble fertilizer outside the coarse fertilizer particles to form a finished product strawberry color value repairing fertilizer, and sending the finished product strawberry color value repairing fertilizer to a finished product package.
EXAMPLE seven
The strawberry color value repairing fertilizer comprises the following components in parts by weight: 100 parts of ammonium sulfate, 32 parts of triple superphosphate, 253 parts of urea, 200 parts of superphosphate, 20 parts of calcium magnesium phosphate fertilizer, 320 parts of potassium sulfate, 10 parts of borax, 18 parts of trace element package, 10 parts of polyamine peptide and 0.5 part of amino acid-containing water-soluble fertilizer.
The amino acid-containing water-soluble fertilizer is a mixture of cyclic dipeptide, pyrimidine nucleoside, amino acid and sugar, and the weight ratio of cyclic dipeptide: pyrimidine nucleosides: amino acids: the sugars were mixed 1:1:1: 1.
The polyamine peptide is a mixture of polyglutamic acid, active amino acid, organic matters and crude plant protein, wherein the weight ratio of the polyglutamic acid: active amino acids: organic matter: crude protein was mixed 3:9:25: 70.
In the trace element package, according to the weight ratio, the weight of the chelated zinc accounts for 42%, the weight of the chelated manganese accounts for 23%, the weight of the boric acid accounts for 33%, and the weight of the ammonium heptamolybdate accounts for 2%.
The nutrient content of the strawberry color value restoration fertilizer is 35%, and the nutrient content is N: p2O5：K2O ═ 14: 5: 16, compared to the conventional fertilizer N: p2O5：K2O15: 15: 15, the nutrient content is reduced, the cost is reduced, but after other raw materials are reasonably matched, the growth of crops is not affected after fertilization.
The preparation method comprises the following steps:
s1, weighing 100 kg of ammonium sulfate, 32 kg of heavy superphosphate, 200 kg of superphosphate, 20 kg of calcium magnesium phosphate fertilizer, 320 kg of potassium sulfate and 10 kg of borax by material proportioning and weighing, and conveying to a mixing tank;
s2, weighing 253 kilograms of molten urea from the urea process and conveying the molten urea to a mixing tank; the sequence of S1 and S2 is not fixed, and can be carried out simultaneously or sequentially, so that the mixing result is not influenced;
mixing the raw materials in S3, S1 and S2 in a mixing tank, and then conveying the mixture to a granulator through a potential difference, wherein the mixture flows out from the bottom of the mixing tank;
s4, spraying the mixture in the step S3 from the top of the granulating tower by a granulator through a rotating centrifugal force to form a coarse fertilizer, and feeding the coarse fertilizer into a cooling system;
s5, preparing cyclic dipeptide according to the mass ratio: pyrimidine nucleosides: amino acids: mixing the sugar in a ratio of 1:1:1:1 to obtain the amino acid-containing water-soluble fertilizer; the weight ratio of polyglutamic acid: active amino acids: organic matter: mixing crude protein 3:9:25:70 to obtain polyamine peptide; chelating zinc according to the mass ratio: chelating manganese: boric acid: mixing heptamolybdic acid (42: 23:33: 2) to obtain a trace element package;
s6, cooling the coarse fertilizer by air cooling of an empty tower of a cooling system, screening out coarse fertilizer particles with qualified particle sizes, sending the coarse fertilizer particles to a coating machine, weighing 10 kg of polyamine peptide, 18 kg of trace element package and 0.5 kg of amino acid-containing water-soluble fertilizer by a batching scale, mixing the polyamine peptide, the trace element package, the amino acid-containing water-soluble fertilizer and liquid coating oil, adding the mixture into the coating machine, wrapping the polyamine peptide, the trace element package and the amino acid-containing water-soluble fertilizer outside the coarse fertilizer particles to form a finished product strawberry color value repairing fertilizer, and sending the finished product strawberry color value repairing fertilizer to a finished product package.
Comparative example 1
The strawberry color value repairing fertilizer comprises the following components in parts by weight: 100 parts of ammonium sulfate, 32 parts of triple superphosphate, 253 parts of urea, 200 parts of superphosphate, 20 parts of calcium magnesium phosphate fertilizer, 320 parts of potassium sulfate, 10 parts of borax and 18 parts of trace element package.
In the trace element package, according to the weight proportion, the weight of the chelated zinc accounts for 44%, the weight of the chelated manganese accounts for 25%, the weight of the boric acid accounts for 29%, and the weight of the ammonium heptamolybdate accounts for 2%.
The nutrient content of the strawberry color value restoration fertilizer is 35%, and the nutrient content is N: p2O5：K2O＝14：5：16。
The preparation method comprises the following steps:
s1, weighing 100 kg of ammonium sulfate, 32 kg of heavy superphosphate, 200 kg of superphosphate, 20 kg of calcium magnesium phosphate fertilizer, 320 kg of potassium sulfate and 10 kg of borax by material proportioning and weighing, and conveying to a mixing tank;
s2, weighing 253 kilograms of molten urea from the urea process and conveying the molten urea to a mixing tank; the sequence of S1 and S2 is not fixed, and can be carried out simultaneously or sequentially, so that the mixing result is not influenced;
mixing the raw materials in S3, S1 and S2 in a mixing tank, and then conveying the mixture to a granulator through a potential difference, wherein the mixture flows out from the bottom of the mixing tank;
s4, spraying the mixture in the step S3 from the top of the granulating tower by a granulator through a rotating centrifugal force to form a coarse fertilizer, and feeding the coarse fertilizer into a cooling system;
s5, chelating zinc according to the mass ratio: chelating manganese: boric acid: mixing 44:25:29:2 heptamolybdic acid to obtain a trace element package;
s6, cooling the coarse fertilizer by air cooling of an empty tower of a cooling system, screening out coarse fertilizer particles with qualified particle sizes, sending the coarse fertilizer particles to a film coating machine, weighing 18 kg of trace element bags by a batching scale, mixing the trace element bags with liquid coating oil, adding the mixture into the film coating machine, enabling raw materials in the trace element bags to wrap the coarse fertilizer particles to form finished fertilizer, and sending the finished fertilizer to finished product packaging.
Comparative example No. two
The strawberry color value repairing fertilizer comprises the following components in parts by weight: 100 parts of ammonium sulfate, 32 parts of triple superphosphate, 253 parts of urea, 200 parts of superphosphate, 20 parts of calcium magnesium phosphate fertilizer, 320 parts of potassium sulfate and 10 parts of borax.
The nutrient content of the strawberry color value restoration fertilizer is 35%, and the nutrient content is N: p2O5：K2O＝14：5：16。
The preparation method comprises the following steps:
s1, weighing 100 kg of ammonium sulfate, 32 kg of heavy superphosphate, 200 kg of superphosphate, 20 kg of calcium magnesium phosphate fertilizer, 320 kg of potassium sulfate and 10 kg of borax by material proportioning and weighing, and conveying to a mixing tank;
s2, weighing 253 kilograms of molten urea from the urea process and conveying the molten urea to a mixing tank; the sequence of S1 and S2 is not fixed, and can be carried out simultaneously or sequentially, so that the mixing result is not influenced;
mixing the raw materials in S3, S1 and S2 in a mixing tank, and then conveying the mixture to a granulator through a potential difference, wherein the mixture flows out from the bottom of the mixing tank;
s4, spraying the mixture in the step S3 from the top of the granulating tower by a granulator through a rotating centrifugal force to form a coarse fertilizer, and feeding the coarse fertilizer into a cooling system;
and S5, cooling the coarse fertilizer by air cooling of an empty tower of a cooling system, screening out coarse fertilizer particles with qualified particle sizes, sending the coarse fertilizer particles to a film coating machine, adding liquid coating oil into the film coating machine to form a finished fertilizer, and sending the finished fertilizer to a finished product package.
The strawberry color value restoration fertilizers prepared in the first to seventh examples were fertilized, and the fertilizers prepared in the first and second comparative examples were fertilized, wherein the strawberry variety is a warm strawberry variety, Miaoxiangqi, bred by Shandong agriculture university, and the results are as follows:
Figure BDA0002611390220000101
Figure BDA0002611390220000111
the first to third examples are full of raw materials without adding the trace element package, and the fourth to seventh examples are full of raw materials, namely the first comparative example is not added with the polyamine peptide and the amino acid-containing water-soluble fertilizer, and the second comparative example is not added with the polyamine peptide, the trace element package and the amino acid-containing water-soluble fertilizer. As can be clearly seen from the above table, the four to seven examples with complete components have the highest per mu yield, the heaviest average single fruit weight, the lowest ugly fruit rate and the lowest hollow rate in one year, which indicates that after the polyamine peptide, the trace element package and the amino acid-containing water-soluble fertilizer are added, the fertilizer improves the acid-base environment of soil, promotes the root growth of strawberry crops, improves the nutrient absorption of the crops under the condition of reasonable component ratio, thereby avoiding the hollow phenomenon of the strawberries and effectively avoiding the growth deformity of the strawberries; although the yield per mu per year is not as good as that of the four to seven full-component examples, the yield per mu per year is higher than that of the first and second comparative examples, and the ugly fruit rate and the hollow rate of the samples are greatly reduced compared with those of the first and second comparative examples, which shows that the polyamine peptide and the amino acid-containing water-soluble fertilizer are added to effectively improve the nutrient absorption of crops, avoid the hollow phenomenon of strawberries and avoid the growth deformity of strawberries; the first comparative example only adds the trace element package, can also correspondingly improve the yield per mu per year to be higher than that of the second comparative example, a single fruit also has certain weight gain effect, but the hollowness and the deformity of the crops can not be well avoided, and the ugly fruit rate and the hollowness rate are only higher than those of the second comparative example which does not add any trace element package; and in the comparative example II, the polyamine peptide, the trace element package and the amino acid-containing water-soluble fertilizer are not added, so that the yield per mu per year is lowest, the weight of a single fruit is lowest, the nutrient absorption of crops is poor, the ugly fruit rate and the hollow rate are also highest, and the effect of adding the polyamine peptide, the trace element package and the amino acid-containing water-soluble fertilizer on the growth and the color value adjustment of the crops is shown.
In conclusion, the invention utilizes the reasonable proportion and the cooperation of the components, can improve the acid-base environment of soil after being used, has the effect of promoting the root growth of strawberry crops, ensures that the main roots and hairy roots of the strawberries are more, and improves the nutrient absorption of the crops, thereby avoiding the hollow phenomenon of the strawberries, effectively repairing the soil environment and avoiding the growth deformity of the strawberries.
Compared with the prior art, the strawberry cultivation method has the advantages that the uniform growth of strawberry crops is promoted, the morbidity of strawberries is reduced, and the planted strawberries are not deformed, so that the finally obtained strawberries are more nutritious, have better mouthfeel and higher appearance color value, and are more easily accepted by markets.
Finally, the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all of them should be covered in the claims of the present invention.

Claims (10)

1. The strawberry color value repairing fertilizer is characterized by comprising the following components in parts by weight: 90-110 parts of ammonium sulfate, 22-42 parts of triple superphosphate, 263 parts of urea 243-.
2. The strawberry color restoration fertilizer as claimed in claim 1, wherein the strawberry color restoration fertilizer comprises, by weight, 90-110 parts of ammonium sulfate, 22-42 parts of triple superphosphate, 263 parts of urea 243-.
3. The strawberry color restoration fertilizer according to claim 2, comprising the following components in parts by weight: 100 parts of ammonium sulfate, 32 parts of triple superphosphate, 253 parts of urea, 200 parts of superphosphate, 20 parts of calcium magnesium phosphate fertilizer, 320 parts of potassium sulfate, 10 parts of borax, 18 parts of trace element package, 10 parts of polyamine peptide and 0.5 part of amino acid-containing water-soluble fertilizer, wherein the nutrient content of the strawberry color value restoration fertilizer is 35%.
4. A strawberry color number restoration fertilizer according to any one of claims 1 to 3, wherein the amino acid-containing water-soluble fertilizer is a mixture of cyclic dipeptide, pyrimidine nucleoside, amino acid and sugar.
5. A strawberry color restoration fertilizer according to any one of claims 1 to 3, wherein the polyamine peptide is a mixture of polyglutamic acid, active amino acids, organic matter and crude plant proteins.
6. The strawberry color number restoration fertilizer according to claim 2 or 3, wherein the trace element package is a mixture of zinc chelate, manganese chelate, boric acid and ammonium heptamolybdate.
7. The strawberry color number restoration fertilizer of claim 6, wherein in the trace element package, the chelated zinc accounts for 40% -45% by weight, the chelated manganese accounts for 23% -28% by weight, the boric acid accounts for 29% -33% by weight, and the ammonium heptamolybdate accounts for 1% -2% by weight.
8. The preparation method of the strawberry color number restoration fertilizer according to claim 1, wherein the method is carried out according to the following steps:
s1, preparing 90-110 parts of ammonium sulfate, 22-42 parts of triple superphosphate, 263 parts of urea 243-;
s2: granulating the mixed product in the S1 to prepare a coarse fertilizer;
s3, coating 8-12 parts of polyamine peptide and 0.4-0.6 part of amino acid-containing water-soluble fertilizer in the crude fertilizer in the S2 to form a finished product.
9. The preparation method of the strawberry color restoration fertilizer according to claim 2, wherein the method comprises the following steps:
s1, preparing 90-110 parts of ammonium sulfate, 22-42 parts of triple superphosphate, 263 parts of urea 243-;
s2: granulating the mixed product in the S1 to prepare a coarse fertilizer;
s3, coating 8-28 parts of trace element package, 8-12 parts of polyamine peptide and 0.4-0.6 part of amino acid-containing water-soluble fertilizer in the crude fertilizer in the S2 to form a finished product, wherein the nutrient content of the finished product is 30%.
10. The method for preparing a strawberry color number restoration fertilizer according to claim 8 or 9, wherein the step S1 comprises:
s10: preparing 90-110 parts of ammonium sulfate, 22-42 parts of triple superphosphate, 190-210 parts of calcium superphosphate, 10-30 parts of calcium magnesium phosphate fertilizer, 330 parts of potassium sulfate and 310-12 parts of borax by weight part, and mixing;
s11: preparing 243 and 263 parts by weight of urea;
s12: the urea in S11 was mixed with the product from S10.
CN202010755408.5A 2020-07-31 2020-07-31 Strawberry color value repairing fertilizer and preparation method thereof Pending CN112010712A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113354478A (en) * 2021-06-01 2021-09-07 华强化工集团股份有限公司 Compound fertilizer for houttuynia cordata and preparation method thereof
CN113416103A (en) * 2021-06-03 2021-09-21 华强化工集团股份有限公司 Composite fertilizer for rape and preparation method thereof
CN113416105A (en) * 2021-06-28 2021-09-21 华强化工集团股份有限公司 Water-soluble fertilizer for increasing yield of cabbages and preparation method thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107056500A (en) * 2017-06-07 2017-08-18 四川安达农森科技股份有限公司 One kind promotes photosynthesis liquid fertilizer
CN109095999A (en) * 2018-10-18 2018-12-28 山东福瑞达生物科技有限公司 A kind of strawberry special complex fertilizer of the polyglutamic acid containing low molecular weight and preparation method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107056500A (en) * 2017-06-07 2017-08-18 四川安达农森科技股份有限公司 One kind promotes photosynthesis liquid fertilizer
CN109095999A (en) * 2018-10-18 2018-12-28 山东福瑞达生物科技有限公司 A kind of strawberry special complex fertilizer of the polyglutamic acid containing low molecular weight and preparation method thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113354478A (en) * 2021-06-01 2021-09-07 华强化工集团股份有限公司 Compound fertilizer for houttuynia cordata and preparation method thereof
CN113416103A (en) * 2021-06-03 2021-09-21 华强化工集团股份有限公司 Composite fertilizer for rape and preparation method thereof
CN113416105A (en) * 2021-06-28 2021-09-21 华强化工集团股份有限公司 Water-soluble fertilizer for increasing yield of cabbages and preparation method thereof

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