CN112006998A - Novel rumen bypass vitamin B6Preparation method and application thereof - Google Patents
Novel rumen bypass vitamin B6Preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
- A61K31/4415—Pyridoxine, i.e. Vitamin B6
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/5005—Wall or coating material
- A61K9/5021—Organic macromolecular compounds
- A61K9/5052—Proteins, e.g. albumin
- A61K9/5057—Gelatin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/14—Drugs for genital or sexual disorders; Contraceptives for lactation disorders, e.g. galactorrhoea
Abstract
The invention relates to the technical field of rumen bypass vitamins, and particularly discloses a novel rumen bypass vitamin B6The preparation method comprises the steps of preparing core material solution, preparing sodium carboxymethyl cellulose solution and preparing gelatin solutionAdding a paraffin mixed solution, a sodium carboxymethylcellulose solution and a gelatin solution into the paraffin mixed solution for emulsification, dissolving ethyl cellulose and polyethylene, mixing the solutions for high-speed dispersion, cooling for crystallization, washing and filtering crystallized particles, freeze-drying and the like; the invention uses special treatment means to treat the existing vitamin B6Preparing a novel rumen bypass vitamin B6So that the prepared novel rumen bypass vitamin B6The degradation rate in rumen is low, and the rumen can reach the small intestine and be fully utilized; and the research shows that a proper amount of novel rumen-bypass vitamin B is added into the daily ration of the dairy cow6Its therapeutic effect is obvious, and said tumor-treating vitamin B6Can realize rapid milk reduction, repair the function of mammary gland, improve economic benefit and is worthy of popularization and application.
Description
Technical Field
The invention relates to the technical field of rumen bypass vitamins, in particular to a novel rumen bypass vitamin B6A preparation method and application thereof.
Background
During the production process of the dairy cow, a large amount of nutrient substances are consumed and the body is easy to fatigue and damage due to long-time lactation and in-vivo fetal growth and development. In order to make the fetus healthy and restore the body condition of the cow, the rumen and mammary gland of the cow have a repair process, and the cow needs to be stopped from lactation in an artificial control mode in the last 2 months of pregnancy of the cow, and the process is called dry milk. The scientific and reasonable dry milk can prolong the service life of the milk cow, keep the milk cow in the optimal state, lay a good foundation for the full play of the production performance of the next period of lactation, reproduction and the like, and promote the milk cow to produce the maximum economic benefit. During the dry period, the turnover of breast tissue does not begin until all of the milk in the breast has been absorbed, which requires a great deal of energy and energy to be expended. If the milk yield of the cow is less than 15 liters when the milk is dry, the process needs about one week; if the dry milk is produced in an amount greater than 15 liters, the absorption of the milk in the udder will take longer and the teat orifice will not seal too well, possibly resulting in infection of the udder and thus mastitis. Therefore, the milk yield of the dairy cows is actively reduced before and after the dry milk, and the method is very important for ensuring the dry milk effect.
Vitamin B6Also known as pyridoxine, which includes pyridoxine, pyridoxal and pyridoxamine, exists in vivo in the form of phosphate esters, and is a water-soluble vitamin. Vitamin B6Is a component of certain coenzymes and is involved in various metabolic reactions, particularly has close relation with amino acid metabolism. In the medical world, vitamin B6Can be used for treating delactation of lactating women, and has remarkable curative effect and no adverse reaction, and its action mechanism may play a coenzyme role in decarboxylation and transamination to increase dopa to dopa in hypothalamusConversion of amine, dopamine may be a prolactin inhibitory factor. However, vitamin B6The treatment for cow's milk withdrawal is rarely reported because of the action of rumen microorganisms if vitamin B is added directly to the cow's ration6Most of vitamin B6Will degrade in the rumen and will be available to the small intestine for little use. Therefore, the vitamin B is not treated in the prior art6Most of vitamin B existing in the process of directly adding dairy cow ration for delactation application6Can be degraded in rumen and has low utilization rate by small intestine, and a novel rumen-passing vitamin B treated by special process is designed6And the application of the composition in the milk returning of dairy cows are technical problems to be solved.
Disclosure of Invention
The invention aims to provide a novel rumen-bypass vitamin B6Preparation method and rumen bypass vitamin B6The application of the vitamin B in the rumen bypass of the milk cow6The application of the milk cow delactation provides a certain theoretical basis.
The invention is realized by the following technical scheme:
novel rumen bypass vitamin B6The preparation method comprises the following preparation steps:
1) preparing a core material solution: taking appropriate amount of vitamin B6Placing the mixture into a reagent bottle containing purified water, then placing the mixture into a constant-temperature water bath shaking table, heating and dissolving the mixture to obtain a solution A after the mixture is fully mixed and dissolved;
2) preparing a sodium carboxymethyl cellulose solution: weighing 1 part by weight of sodium carboxymethylcellulose, adding the sodium carboxymethylcellulose into 80-120 parts by weight of deionized water, heating and stirring until the sodium carboxymethylcellulose is completely dissolved to obtain a solution B, pouring the solution B into a high-pressure homogenizer, homogenizing under high pressure of 40-60 MPa, and repeating the high-pressure homogenization for 2-4 times;
3) preparing a gelatin solution: weighing 1 part by weight of gelatin, adding the gelatin into 80-120 parts by weight of deionized water, heating and stirring until the gelatin is completely dissolved to obtain a solution C, pouring the solution C into a high-pressure homogenizer, homogenizing under high pressure of 40-60 Mpa, and repeating the high-pressure homogenization for 2-4 times;
4) preparing a paraffin mixed solution: taking 1000 parts of liquid paraffin and 1-3 parts of sorbitan monooleate according to the volume ratio, and uniformly mixing the liquid paraffin and the sorbitan monooleate to obtain a solution D;
5) mixing and stirring the solution B prepared in the step 2 and the solution C prepared in the step 3 according to the volume of 1:1, then adding the stirred solution into the solution D, wherein the volume ratio of the solution D to the solution B to the solution C is 1:2:2, ultrasonically emulsifying for 5-10 min, immediately transferring into a low-temperature water bath, and stirring for 30-60 min at the temperature of 20-25 ℃ to obtain a solution E;
6) adding ethyl cellulose and polyethanol into an organic solvent according to the mass ratio of 1:1, heating and stirring until the ethyl cellulose and the polyethanol are completely dissolved, homogenizing under high pressure of 40-60 Mpa, and repeating the high-pressure homogenization for 2-4 times to obtain a solution F;
7) mixing the solution A prepared in the step 1 and the solution E prepared in the step 5 in a ratio of (8-10): 5, dispersing the mixture in a high-speed refiner at a rotating speed of 6000 to 10000r/min for about 30 to 50s, repeating the high-speed dispersion treatment for 2 to 4 times, and then using an electric stirrer at a temperature of between 60 and 85 ℃ for 20 to 30 min;
8) adding the solution F prepared in the step 6 into the mixed solution stirred in the step 7, wherein the volume ratio of the solution F to the mixed solution stirred in the step 7 is 3: (6-8), continuously stirring for 20-30 min, slowly reducing the temperature to reduce the solubility of the ethyl cellulose for cooling and solidification to obtain granular crystals, washing and filtering with an organic solvent, and freeze-drying in a vacuum freeze-drying machine after washing and filtering to obtain rumen-bypass vitamin B6。
Preferably, vitamin B is present in step 16The weight ratio of the purified water to the purified water is 2: 3.
preferably, the heating dissolution temperature in the step 1 is 80 ℃, and the operation is carried out for 30 min.
Preferably, the heating and stirring temperature in the step 2 is 80 ℃, and the stirring speed is 200-400 r/min.
Preferably, the heating and stirring temperature in the step 3 is 50 ℃, and the stirring speed is 200-400 r/min.
Preferably, the heating and stirring temperature in the step 6 is 80 ℃, and the stirring speed is 400-600 r/min.
Preferably, the organic solvent in step 6 and step 8 is cyclohexane.
Preferably, the diameter of the granular crystal in the step 8 is 0.8-1 mm.
A novel rumen-protected vitamin B prepared by the above preparation method6The application in cow colostrum is provided.
Preferably, the novel rumen bypass vitamin B6Feeding the cows in the dry period by adding them to the basal ration.
Has the advantages that:
1. the invention uses special treatment means to treat the existing vitamin B6Preparing a novel rumen bypass vitamin B6So that the prepared novel rumen bypass vitamin B6The degradation rate in rumen is low, and the rumen can reach the small intestine and be fully utilized; and through research, the rumen-bypass vitamin B using the invention is found6The milk cow feed is put in the basic daily ration to feed the milk cow in the dry period, so that the milk of the milk cow can be reduced in a short period, and a solid foundation is laid for the full play of the production performances of the milk cow in the next period, such as lactation, reproduction and the like.
2. The novel rumen-bypass vitamin B prepared by the invention6After passing relevant experiments, the method can be obtained by adding a proper amount of novel rumen-bypass vitamin B into daily ration of the dairy cow6Its therapeutic effect is obvious, and said tumor-treating vitamin B6Can realize rapid milk reduction, repair the function of mammary gland, improve economic benefit and is worthy of popularization and application.
Detailed Description
Example 1
Novel rumen bypass vitamin B6The preparation method comprises the following eight preparation steps:
1) preparing a core material solution:
per vitamin B6And the purified water is 2: 3 the weight ratio of proper vitamin B6Is placed in a reagent filled with purified waterPutting the mixture into a bottle, putting the bottle into a constant-temperature water bath shaking table, heating the bottle to 80 ℃, and running the bottle for 30min to ensure that the solution A is fully mixed and dissolved to obtain a solution A.
2) Preparing a sodium carboxymethyl cellulose solution:
accurately weighing 1 part by weight of CMC (sodium carboxymethylcellulose), slowly adding into 99 parts by weight of deionized water, stirring at a stirring speed of 350r/min and at a temperature of 80 ℃ by using an electric stirrer until the CMC is completely dissolved to obtain a solution B, pouring the solution B into a high-pressure homogenizer, homogenizing under a high pressure of 50MPa, and repeating the high-pressure homogenization treatment for 3 times by using the same parameters.
3) Preparing a gelatin solution:
accurately weighing 1 part by weight of gelatin, slowly adding the gelatin into 99 parts by weight of deionized water, stirring the gelatin at a stirring speed of 350r/min and a temperature of 50 ℃ by using an electric stirrer until the gelatin is completely dissolved to obtain a solution C, homogenizing the solution C under the same conditions (namely 50 MPa) as the step 2, and repeating the high-pressure homogenization treatment for 3 times by using the same parameters.
4) Preparing a paraffin mixed solution:
selecting liquid paraffin according to paraffin HLB, taking 1000 parts of liquid paraffin and 1 part of sorbitan monooleate (span-80) according to volume ratio, and uniformly mixing the liquid paraffin and the sorbitan monooleate to obtain a solution D.
5) Mixing and stirring the solution B prepared in the step 2 and the solution C prepared in the step 3 according to the volume of 1:1, then adding the stirred solution into the solution D, wherein the volume ratio of the solution D to the solution B to the solution C is 1:2:2, immediately carrying out ultrasonic emulsification for 10min, and then immediately transferring into a low-temperature water bath kettle to stir for 30min at the temperature of 22 ℃ to obtain a solution E.
6) Adding ethyl cellulose and polyethanol into an organic solvent (cyclohexane is selected as the organic solvent) according to the mass ratio of 1:1, heating to 80 ℃, stirring at the rotating speed of 500r/min until the ethyl cellulose and the polyethanol are completely dissolved, then homogenizing under high pressure of 50MPa, and repeating the high-pressure homogenizing treatment for 3 times according to the same parameters to obtain a solution F.
7) Mixing solution a prepared in step 1 with solution E prepared in step 5 at a ratio of 9: 5, dispersing in a high-speed homogenizer at a rotation speed of 10000r/min for about 30s, repeating the high-speed dispersion treatment 3 times, and then stirring with an electric stirrer at 80 ℃ for 20 min.
8) Adding the solution F prepared in the step 6 into the mixed solution stirred in the step 7, wherein the volume ratio of the solution F to the mixed solution stirred in the step 7 is 3: 7, continuously stirring for 25min, slowly reducing the temperature to reduce the solubility of the ethyl cellulose for cooling and solidifying to obtain granular crystals with the diameter of 0.8-1 mm, washing and filtering by using an organic solvent (the organic solvent also selects cyclohexane), freeze-drying in a vacuum freeze dryer after washing and filtering, and finally obtaining the rumen-bypass vitamin B6。
The novel rumen-protected vitamin B prepared in example 16Can be used for cow milk recovery, and can be prepared into B in specific application6Added to the basal ration in the appropriate amount and fed to the cows on the dry period.
For the novel rumen-protected vitamin B prepared in example 16Evaluation of rumen bypass rate performance:
rumen bypass vitamin B prepared in example 1 was measured by the rumen nylon bag method using 4 holstein lactating cows with permanent rumen fistula as test animals6And uncoated rumen bypass vitamin B6Rumen degradation rate at 2, 6, 12, 24 hours 4 culture points. The results of the measurements of 1 plastic tube (2 nylon bags) per cow per time point are shown in table 1 below, where the data in table 1 below is the average of 8 replicates.
Specifically, the rumen degradation rate is calculated as follows:
rumen degradation rate = (rumen-passing vitamin B at certain culture time point)6Degradation amount of the vitamin B6The weight)' 100%;
rumen bypass vitamin B6Degradation amount at a certain time point = corrected bagged rumen bypass vitamin B6Weight of-rumen bypass vitamin B at a certain incubation time6The weight of (c);
correcting and bagging rumen bypass vitamin B6Weight = practically bagging rumen bypass vitamin B6' (1-rumen-protected vitamin B)6Bag escape rate);
rumen bypass vitamin B6Escape rate (%) in packaging = [ (rumen-protected vitamin B in blank test)6By-pass vitamin B in weight-blank test of6Weight of residue) ¸ rumen bypass vitamin B in blank test6Weight of (2)]´100%;
The results of the rumen degradation rate measurement are shown in Table 1.
Table 1: rumen-bypass vitamin B determined by nylon bag method6Degradation ratio (%)
2(h) | 6(h) | 12(h) | 24(h) | |
Example 1 | 8.25 | 12.47 | 16.21 | 22.29 |
Uncoated vitamins | 94.81 | 96.31 | 98.49 | 99.04 |
As can be seen from the data in Table 1, vitamin B was not treated with coating6Substantially degraded at 12h, whereas example 1 prepared rumen bypass vitamin B6The degradation rate of the novel rumen bypass vitamin B treated by the preparation method is 16.21 percent after the novel rumen bypass vitamin B is cultured in rumen for 12 hours6Its rumen-bypass rate is up to 80%, and its effect is obvious.
For the novel rumen-protected vitamin B prepared in example 16Evaluation of the delactation effect on cows:
1. materials and methods
1.1 selection, grouping and management of test cows
Selecting 40 cows (7 days before dry milk) which have similar weight, 2-4 fetuses and good body conditions and normal breasts and milk and are close to a dry milk period, randomly dividing the cows into 2 groups, wherein each group comprises 20 cows, and the group I is a control group and is fed with basic ration; group II rumen bypass vitamin B prepared in example 1 was added to basal diet 10g per day6Test period 7 days.
1.2 test materials
The test material was rumen bypass vitamin B prepared in example 16。
1.3 items of examination
Recording of milk yield: the milk yield of the cows before the test and on days 1-7 was recorded.
1.4 data processing
Data analysis was performed using SPSS24.0 software ANOVN (one-way analysis of variance), and the experimental data are presented as means. + -. standard deviation (x. + -. SD).
2. Results and analysis
2.1 rumen bypass vitamin B6Delactation effect on cow
Table 2: rumen bypass vitamin B6Delactation effect on cow
Note: the data in the same row marked with lower case letters completely different indicates that the difference is significant (P < 0.05), and the data in the same row marked with the same letter or without the letter indicates that the difference is not significant (P > 0.05).
In actual production, the daily milk yield of the cows is generally reduced to 4-5 kg by measures of reducing the daily ration nutrition of the cows, reducing the milking frequency, enhancing the cow motion and the like, and then the cows are dried. As can be seen from Table 2, the average milk yield on day 7 of group I was 10.25kg and did not meet the dry milk standard, while the average milk yield on balance 5 of group II was 5.28kg and meets the dry milk standard, and the rumen bypass vitamin B prepared in example 1 was added to the basal ration of cows as determined by the above experiment6It has obvious effect of stopping milk of milk cow, and the tumor-treating vitamin B6Can realize rapid milk reduction, repair the function of mammary gland, improve economic benefit and is worthy of popularization and application.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. Novel rumen bypass vitamin B6The preparation method is characterized by comprising the following preparation steps:
1) taking appropriate amount of vitamin B6Placing the mixture into a reagent bottle containing purified water, then placing the mixture into a constant-temperature water bath shaking table, heating and dissolving the mixture to obtain a solution A after the mixture is fully mixed and dissolved;
2) weighing 1 part by weight of sodium carboxymethylcellulose, adding the sodium carboxymethylcellulose into 80-120 parts by weight of deionized water, heating and stirring until the sodium carboxymethylcellulose is completely dissolved to obtain a solution B, pouring the solution B into a high-pressure homogenizer, homogenizing under high pressure of 40-60 MPa, and repeating the high-pressure homogenization for 2-4 times;
3) weighing 1 part by weight of gelatin, adding the gelatin into 80-120 parts by weight of deionized water, heating and stirring until the gelatin is completely dissolved to obtain a solution C, pouring the solution C into a high-pressure homogenizer, homogenizing under high pressure of 40-60 Mpa, and repeating the high-pressure homogenization for 2-4 times;
4) taking 1000 parts of liquid paraffin and 1-3 parts of sorbitan monooleate according to the volume ratio, and uniformly mixing the liquid paraffin and the sorbitan monooleate to obtain a solution D;
5) mixing and stirring the solution B prepared in the step 2 and the solution C prepared in the step 3 according to the volume of 1:1, then adding the stirred solution into the solution D, wherein the volume ratio of the solution D to the solution B to the solution C is 1:2:2, ultrasonically emulsifying for 5-10 min, immediately transferring into a low-temperature water bath, and stirring for 30-60 min at the temperature of 20-25 ℃ to obtain a solution E;
6) adding ethyl cellulose and polyethanol into an organic solvent according to the mass ratio of 1:1, heating and stirring until the ethyl cellulose and the polyethanol are completely dissolved, homogenizing under high pressure of 40-60 Mpa, and repeating the high-pressure homogenization for 2-4 times to obtain a solution F;
7) mixing the solution A prepared in the step 1 and the solution E prepared in the step 5 in a ratio of (8-10): 5, dispersing the mixture in a high-speed refiner at a rotating speed of 6000 to 10000r/min for 30 to 50s, repeating the high-speed dispersion treatment for 2 to 4 times, and then using an electric stirrer at a temperature of between 60 and 85 ℃ for 20 to 30 min;
8) adding the solution F prepared in the step 6 into the mixed solution stirred in the step 7, wherein the volume ratio of the solution F to the mixed solution stirred in the step 7 is 3: (6-8), continuously stirring for 20-30 min, slowly reducing the temperature to reduce the solubility of the ethyl cellulose for cooling and solidification to obtain granular crystals, washing and filtering with an organic solvent, and freeze-drying in a vacuum freeze-drying machine after washing and filtering to obtain rumen-bypass vitamin B6。
2. Novel rumen-protected vitamin B as claimed in claim 16The preparation method is characterized by comprising the following steps: vitamin B in said step 16The weight ratio of the purified water to the purified water is 2: 3.
3. novel rumen-protected vitamin B as claimed in claim 16The preparation method is characterized in thatIn the following steps: the heating and dissolving temperature in the step 1 is 80 ℃, and the operation is carried out for 30 min.
4. Novel rumen-protected vitamin B as claimed in claim 16The preparation method is characterized by comprising the following steps: the heating and stirring temperature in the step 2 is 80 ℃, and the stirring speed is 200-400 r/min.
5. Novel rumen-protected vitamin B as claimed in claim 16The preparation method is characterized by comprising the following steps: the heating and stirring temperature in the step 3 is 50 ℃, and the stirring speed is 200-400 r/min.
6. Novel rumen-protected vitamin B as claimed in claim 16The preparation method is characterized by comprising the following steps: the heating and stirring temperature in the step 6 is 80 ℃, and the stirring speed is 400-600 r/min.
7. Novel rumen-protected vitamin B as claimed in claim 16The preparation method is characterized by comprising the following steps: the organic solvent in the step 6 and the organic solvent in the step 8 are both cyclohexane.
8. Novel rumen-protected vitamin B as claimed in claim 16The preparation method is characterized by comprising the following steps: the diameter of the granular crystal in the step 8 is 0.8-1 mm.
9. A novel rumen-protected vitamin B as claimed in any one of claims 1 to 86Novel rumen-bypass vitamin B obtained by preparation method6The application in cow colostrum is provided.
10. Novel rumen-protected vitamin B as claimed in claim 96Use in cow's milk recovery, characterized in that, the novel rumen-protected vitamin B6Feeding the cows in the dry period by adding them to the basal ration.
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CN112868912A (en) * | 2021-02-02 | 2021-06-01 | 北京东方天合过瘤胃技术研究院有限公司 | Preparation method of novel rumen-bypass glucose oxidase |
CN112868912B (en) * | 2021-02-02 | 2022-05-10 | 北京东方天合过瘤胃技术研究院有限公司 | Preparation method of rumen-bypass glucose oxidase |
CN115380999A (en) * | 2022-08-12 | 2022-11-25 | 北京东方天合生物技术有限责任公司 | Rumen-bypass melatonin composition for improving pregnancy rate of ruminants as well as preparation method and application of rumen-bypass melatonin composition |
CN115380999B (en) * | 2022-08-12 | 2023-08-29 | 北京东方天合生物技术有限责任公司 | Rumen bypass melatonin composition for improving pregnancy rate of ruminant animals as well as preparation method and application thereof |
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