CN115380999A - Rumen-bypass melatonin composition for improving pregnancy rate of ruminants as well as preparation method and application of rumen-bypass melatonin composition - Google Patents
Rumen-bypass melatonin composition for improving pregnancy rate of ruminants as well as preparation method and application of rumen-bypass melatonin composition Download PDFInfo
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Abstract
The application relates to the technical field of feed additives, and particularly discloses a rumen-bypass melatonin composition for improving pregnancy rate, and a preparation method and application thereof, wherein the rumen-bypass melatonin composition comprises a core material and an outer coating layer which are sequentially arranged from inside to outside, and the core material comprises a mass ratio of 1: (2-3): (0.5-0.8) melatonin, corn gluten meal and geniposide; the preparation method comprises the following steps: preparing core material particles: dissolving geniposide in water, adding corn protein powder, mixing and stirring at 35-40 ℃, adding melatonin, mixing and stirring, granulating, and drying to obtain a core material; coating with an outer coating layer: coating the core material to obtain the rumen bypass melatonin composition. The application also discloses the use of the rumen bypass melatonin composition in ruminant additives. The application has the effect of improving the pregnancy rate of the ruminant by melatonin.
Description
Technical Field
The application relates to the technical field of cow pregnancy rate improvement, in particular to a rumen-bypass melatonin composition for improving pregnancy rate and a preparation method and application thereof.
Background
The quality of the reproductive performance of the dairy cows not only affects the increase of the number and the quality of the dairy cows, but also affects the production performance and the economic benefit of the dairy cows, so that the pregnancy rate of the dairy cows is widely regarded by the dairy cows of various countries.
Especially, the low pregnancy rate of the postpartum mating of the dairy cows is always the bottleneck of the development of the dairy industry. Some, but not insignificant, results were obtained by improving the diet and nutritional levels. Researches show that the melatonin is involved in follicular development, oocyte quality and embryo development, and the pregnancy rate of cows can be improved when the melatonin is injected subcutaneously into Holstein cows. However, the subcutaneous injection method is complex in operation and has higher requirements on the injection technology of culturists, and the injection method has higher risk of cross infection, especially in hot summer, the risk of wound infection still exists, and other feeding methods are needed.
However, the direct feeding of melatonin is a problem to be solved urgently, because rumen microorganisms of ruminants can utilize melatonin, the melatonin cannot reach the small intestine as much as possible, absorption and utilization of the melatonin by the ruminants are influenced, and how to further improve the effect of the melatonin on the pregnancy rate of the ruminants is needed.
Disclosure of Invention
In order to improve the effect of melatonin on improving the pregnancy rate of ruminants, the application provides a rumen-bypass melatonin composition for improving the pregnancy rate and a preparation method thereof.
In a first aspect, the rumen bypass melatonin composition for improving pregnancy rate provided by the application adopts the following technical scheme:
the rumen-protected melatonin composition for improving pregnancy rate comprises a core material and an outer coating layer which are sequentially arranged from inside to outside, wherein the core material comprises the following components in percentage by mass: (2-3): (0.5-0.8) melatonin, corn gluten meal and geniposide.
By adopting the technical scheme, the chemical name of the melatonin is N-acetyl-5-hydroxytryptamine, the hormone is secreted by the pineal body and has strong oxidation resistance, the oxidation resistance of the melatonin can protect the corpus luteum from being damaged by oxidative stress, the corpus luteum function is maintained, the ovarian apoptosis is reduced, the hormone acts on corpus luteum cells to generate the progestogen, and the pregnancy rate of the ruminant is further improved.
The applicant finds that the corn protein powder is used as an auxiliary nutritional factor, on one hand, the corn protein powder contains linoleic acid, can promote lipid metabolism and can promote the polymerization of essential amino acid, and probably because the corn protein powder influences the lipid metabolism and the amino acid synthesis, a certain antagonistic effect is generated on the apoptosis of ovarian cells in an animal body, and the normal development of follicles is promoted; on the other hand, the addition of the corn protein powder can ensure that partial protein which cannot be digested by rumen can be better digested and absorbed in small intestine, so that more energy is provided, positive significance is provided for the development and synthesis of oocyte, and positive effects of melatonin on follicular development, oocyte quality and the like are further promoted, so that the pregnancy rate is further improved;
but zein powder leads to zein powder's absorption utilization ratio lower because the water-solubility is poor, geniposide acid is as the modifier in the core material of this application, geniposide acid and zein powder reaction to can realize the modification to zein powder, improve zein powder's water-solubility, thereby promote ruminant to zein powder's absorption and utilization, can be better with melatonin combined action improvement pregnancy rate.
Optionally, the core material further includes genipin and L-hydroxyproline, the mass ratio of genipin to genipin acid is (1.2-1.5), and the mass ratio of genipin to L-hydroxyproline is 1: (0.8-1).
Through adopting above-mentioned technical scheme, L-hydroxyproline acts on corn gluten meal's granule through genipin with L-hydroxyproline as the amino acid of soluble in water in this application to can further improve corn gluten meal's water-soluble, promote the absorption to corn gluten meal. Furthermore, the addition of L-hydroxyproline as a sweet substance can also increase the palatability of the melatonin composition.
Optionally, the geniposide acid is added after being coated with a polyacrylic resin coating, and the addition mass ratio of the polyacrylic resin to the geniposide acid is 1: (0.5-0.8).
By adopting the technical scheme, the genipin is coated by the polyacrylic resin, so that after rumen-bypass melatonin enters the true stomach, genipin and L-hydroxyproline directly act with corn protein powder, and after the polyacrylic resin is dissolved for a period of time, the genipin is released to act with the corn protein powder, so that the action of carboxyl of the genipin and hydroxyl of the genipin and L-hydroxyproline is prevented, the protection of carboxylic acid on genipin chains is realized, and finally, the corn protein powder has better water solubility and is more favorable for absorption.
Optionally, the genipin and the L-hydroxyproline are added after being coated by a coating agent, and the addition mass ratio of the coating agent to the sum of the genipin and the L-hydroxyproline is 1: (1.5-2).
By adopting the technical scheme, the ratio of geniposidic acid to polyacrylic resin is controlled, the sum of the masses of genipin and L-hydroxyproline and the ratio of the coating agent are controlled, so that the thickness of the coating layer of the geniposidic acid is far lower than that of the coating layer of the genipin and the L-hydroxyproline, the genipin and the L-hydroxyproline firstly react with the corn protein powder, and the geniposidic acid is protected.
Optionally, the coating agent for coating genipin and L-hydroxyproline comprises the following components in a mass ratio of 1: (2-3): (0.3-0.5): (0.2-0.4) low ester pectin, shellac, and calcium ascorbate, and calcium carbonate.
By adopting the technical scheme, the coating agent is a mixture of low-ester pectin, shellac, calcium ascorbate and calcium carbonate, the low-ester pectin forms gel when calcium ions are provided by the calcium ascorbate, and after genipin and L-hydroxyproline enter the true stomach, the calcium ascorbate is firstly dissociated in vivo into the ascorbic acid and absorbable calcium, so that the low-ester pectin breaks the gel, and the calcium carbonate reacts under an acidic condition to accelerate the gel breaking and release the genipin, so that the genipin and the L-hydroxyproline with thinner coatings can quickly break the gel to act with the corn gluten meal.
In a second aspect, the present application provides a method for preparing rumen-bypass melatonin for improving pregnancy rate, which adopts the following technical scheme:
a preparation method of rumen-bypass melatonin for improving pregnancy rate comprises the following steps:
preparing core material particles: dissolving geniposide in water, adding corn protein powder, mixing and stirring at 35-40 ℃, adding melatonin, mixing and stirring, granulating, and drying to obtain a core material;
coating with an outer coating layer: coating the core material to obtain the rumen-protected melatonin composition.
By adopting the technical scheme, the geniposide is firstly dissolved in water, then the corn protein powder is added, the geniposide reacts with the corn protein powder to realize hydrophilic modification on the corn protein powder, then the geniposide is mixed with the melatonin to obtain the core material, and finally the rumen-bypass melatonin composition with the core material of the melatonin, the corn protein powder and the geniposide is obtained.
Optionally, the core material further comprises genipin and L-hydroxyproline, the addition mass ratio of genipin to genipin acid is (1.2-1.5), and the addition mass ratio of genipin to L-hydroxyproline is 1: (0.8-1);
in the step of preparing the core material particles, geniposidic acid is dissolved in water, L-hydroxyproline is added, and then corn protein powder and genipin are added.
Optionally, in the step of preparing the core material particles, the geniposidic acid is added after being spray-dried and coated with polyacrylic resin, and the addition mass ratio of the polyacrylic resin to the geniposidic acid is 1: (0.5-0.8).
Optionally, the genipin and the L-hydroxyproline are added after being coated by a coating agent, wherein the coating agent comprises the following components in a mass ratio of 1: (2-3): (0.3-0.5): (0.2-0.4) low-ester pectin, shellac, calcium ascorbate and calcium carbonate, wherein the addition mass ratio of the coating agent to the sum of the masses of genipin and L-hydroxyproline is 1: (1.5-2);
in the core material particle preparation step, the coating operation of genipin and L-hydroxyproline is as follows:
adding calcium carbonate into the shellac solution, and dispersing and stirring to obtain a mixture A;
adding genipin and L-hydroxyproline into the mixture A, and stirring to obtain a mixture B;
adding low-ester pectin into the mixture B, heating and stirring to obtain a mixture C;
and (3) dropwise adding the calcium ascorbate solution into the mixture C, stirring at low temperature, drying and forming to obtain the coated genipin/L-hydroxyproline.
In a third aspect, the present application provides an application of a rumen bypass melatonin composition for increasing the pregnancy rate of ruminants, which adopts the following technical scheme:
use of a rumen bypass melatonin composition for increasing pregnancy rate of ruminant in ruminant additives is provided.
In summary, the present application has the following beneficial effects:
1. according to the application, through the addition of the corn protein powder and the geniposide, the influence of the corn protein powder on apoptosis is promoted by utilizing the antagonistic effect of the corn protein powder on the apoptosis, and the pregnancy rate is improved;
2. in the application, L-hydroxyproline is used as amino acid which is easy to dissolve in water, and the L-hydroxyproline is grafted on particles of corn protein powder through genipin, so that the water solubility of the corn protein powder can be further improved, and the absorption of the corn protein powder is promoted;
3. through the thickness and the coating material to genipin and L-hydroxyproline and genipin acid coating in this application for genipin and L-hydroxyproline act on corn gluten meal earlier, then genipin acid releases back and the effect of corn gluten meal, thereby prevent the carboxyl of genipin acid and the hydroxyl effect of genipin and L-hydroxyproline, realize the protection to carboxylic acid on the genipin glycoside chain, finally corn gluten meal's water-solubility is better, be favorable to the absorption more.
Detailed Description
The present application is further described in detail with reference to the following examples, which are specifically illustrated by the following: the following examples, in which specific conditions are not specified, were carried out according to conventional conditions or conditions recommended by the manufacturer, and the starting materials used in the following examples are available from ordinary commercial sources unless otherwise specified.
The outer coating layer for coating the core material adopts ethyl cellulose, and the outer coating of the core material is realized by adopting a spray drying mode.
Example 1
A preparation method of a rumen bypass melatonin composition for improving pregnancy rate comprises the following steps:
s1, preparing core material particles: dissolving geniposide in water, adding corn protein powder, mixing and stirring at 35-40 deg.C for 30min, adding melatonin, mixing and stirring, granulating, and drying to obtain core material;
wherein, the mass ratio of the geniposide to the water is 2;
s2, coating of an outer coating layer: dissolving ethyl cellulose in ethanol to prepare an ethyl cellulose solution, wherein the mass ratio of the ethyl cellulose to the absolute ethanol is 1.
Example 2
A preparation method of rumen bypass melatonin composition for improving pregnancy rate comprises the following steps:
s1, preparing core material particles: dissolving geniposide in water, adding corn protein powder, mixing and stirring at 40 deg.C for 40min, adding melatonin, mixing and stirring, granulating, and drying to obtain core material;
wherein, the mass ratio of the geniposide to the water is 2;
s2, coating of an outer coating layer: dissolving ethyl cellulose in ethanol to prepare an ethyl cellulose solution, wherein the mass ratio of the ethyl cellulose to the absolute ethanol is 1.
Example 3
A preparation method of rumen bypass melatonin composition for improving pregnancy rate comprises the following steps:
s1, preparing core material particles: dissolving geniposide in water, adding corn protein powder, mixing and stirring at 40 deg.C for 1h, adding melatonin, mixing and stirring, granulating, and drying to obtain core material;
wherein, the mass ratio of the geniposide to the water is 2;
s2, coating of an outer coating layer: dissolving ethyl cellulose in ethanol to prepare an ethyl cellulose solution, wherein the mass ratio of the ethyl cellulose to the absolute ethanol is 1.
Example 4
A method for preparing a rumen bypass melatonin composition for increasing pregnancy rate, which is carried out according to the method of example 2, except that:
the core material in the step S1 is also added with a raw material with the mass ratio of 1:0.9 of genipin and L-hydroxyproline, wherein the addition mass ratio of genipin to genipin acid is 1:1.3;
the specific operation of step S1 is:
dissolving geniposide and L-hydroxyproline in water, adding corn protein powder and genipin, mixing and stirring at 40 deg.C for 40min, adding melatonin, mixing and stirring, granulating, and drying to obtain core material.
Example 5
The preparation method of the rumen bypass melatonin composition for improving pregnancy rate is carried out according to the method in the embodiment 4, and the difference is that geniposidic acid is added after polyacrylic resin spray drying coating, and the adding mass ratio of the polyacrylic resin to the geniposidic acid is 1:0.6;
the coating operation is as follows: dissolving polyacrylic resin in absolute ethyl alcohol with the mass multiple of 2, and then carrying out spray drying on the obtained polyacrylic resin solution on geniposidic acid.
Example 6
A preparation method of a rumen bypass melatonin composition for improving pregnancy rate is carried out according to the method in the embodiment 5, wherein genipin and L-hydroxyproline are added after being coated by a coating agent, and the coating agent comprises the following components in a mass ratio of 1:2.5:0.4:0.3 of low-ester pectin, shellac, calcium ascorbate and calcium carbonate, wherein the mass ratio of the sum of the masses of genipin and L-hydroxyproline to the sum of the low-ester pectin, shellac, calcium ascorbate and calcium carbonate is 1;
in the core material particle preparation step, the coating of genipin and L-hydroxyproline specifically comprises the following steps:
adding calcium carbonate into a shellac solution (obtained by mixing shellac and absolute ethyl alcohol according to a mass ratio of 1;
adding genipin and L-hydroxyproline into the mixture A, and stirring to obtain a mixture B;
adding low-ester pectin into the mixture B, and heating and stirring at 50 ℃ to obtain a mixture C;
and (3) dropwise adding a 15wt% calcium ascorbate solution into the mixture C, stirring at a low temperature of 20 ℃, drying and forming to obtain the coated genipin/L-hydroxyproline.
Example 7
A method for preparing a rumen bypass melatonin composition for improving pregnancy rate, which is carried out according to the method in the embodiment 6, and is characterized in that genipin and L-hydroxyproline are also added after being coated by polyacrylic resin, and the mass ratio of the polyacrylic resin to the sum of the genipin and the L-hydroxyproline is 1;
the coating method comprises the following specific operations: uniformly mixing genipin and L-hydroxyproline, preparing with water, drying to obtain mixed particles, dissolving polyacrylic resin in absolute ethyl alcohol with the mass multiple of 2, and spray-drying the obtained polyacrylic resin solution on the genipin/L-hydroxyproline mixed particles.
Example 8
A rumen bypass melatonin composition for improving pregnancy rate was prepared by the same method as in example 6, except that calcium carbonate was not added to the coating agent coated with genipin and L-hydroxyproline.
Comparative example
Comparative example 1
The preparation method of the rumen bypass melatonin composition for improving the pregnancy rate is carried out according to the method in the embodiment 2, and the difference is that the addition mass ratio of the melatonin, the corn protein powder and the geniposide is 1.
Comparative example 2
A preparation method of a rumen-bypass melatonin composition for improving pregnancy rate is carried out according to the method in example 2, except that genipin is replaced by melatonin and corn protein powder in an equal amount, and the mass ratio of the melatonin to the corn protein powder is the same as that in example 2.
Comparative example 3
A method for preparing a rumen bypass melatonin composition for improving pregnancy rate, which comprises the following steps of example 2, wherein the difference is that geniposide and corn gluten meal are replaced by melatonin with equal amount.
Performance detection
1. Rumen bypass performance detection:
rumen degradation rates (%) of the rumen-protected melatonin compositions prepared in examples 1 to 3 and the uncoated rumen-protected melatonin compositions (core particles obtained in example 2) at four culture points of 2h, 6h, 12h and 24h were measured by the rumen nylon bag method using a holstein lactating cow having a permanent rumen fistula as a test animal, and the results of the rumen degradation rate (%) were shown in table 1 below.
Table 1:
| detecting items | 2h | 6h | 12h | 24h |
| Example 1 | 4.02 | 7.67 | 9.66 | 13.29 |
| Example 2 | 2.98 | 4.46 | 5.89 | 8.92 |
| Example 3 | 5.28 | 8.90 | 12.76 | 15.45 |
| Uncoated melatonin | 90.27 | 92.86 | 98.54 | 98.96 |
As can be seen from table 1 above, the rumen bypass melatonin composition which is not coated in the present application is degraded by more than 95% after 12 hours, whereas the degradation rate of the rumen bypass melatonin composition which is coated in examples 1-3 is reduced to less than 15% after 12 hours in the rumen, and the degradation rate of the rumen bypass composition which is coated is greatly reduced in the rumen.
2. Effect of rumen bypass composition on pregnancy Rate
Healthy and multiparous (2-4) cows with Holstein are selected, and fed with full mixed feed, and the cows can move freely and drink water. The pedometer method determines 360 naturally-estrous Holstein cows, each 30 cows are a test group, and 12 test groups are provided, wherein one test group is a control group, basic daily ration is fed, 50g of rumen-bypass melatonin composition prepared by the methods in examples 1-8 and comparative examples 1-3 is added to the basic daily ration respectively fed to the remaining 11 test groups, and the control group is fed with the basic daily ration without adding rumen-bypass melatonin. After 12h, artificial insemination was performed on the estrus Holstein cows in the test group, pregnancy detection was performed with a B ultrasonic instrument 20-30d after insemination, and the pregnancy rate was recorded in detail, with the results shown in Table 2 below.
Table 2:
| detecting items | Example 1 | Example 2 | Example 3 | Example 4 | Example 5 | Example 6 |
| Percentage of pregnancy/%) | 42.00％ | 44.00％ | 40.00％ | 48.00％ | 55.00％ | 58.00％ |
| Detecting items | Example 7 | Example 8 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Control group |
| Pregnancy rate/%) | 46.00％ | 50.00％ | 40.00％ | 34.00％ | 32.00％ | 28.00％ |
By combining the above table 2 and referring to the detection results of the example 2 and the control group, it can be seen that the pregnancy rate of the dairy cows can be significantly improved when the rumen bypass melatonin composition provided in the application is added to the daily feed; in combination with the detection result of the comparative example 3, it can be seen that when only melatonin is added to the core material of rumen-bypass melatonin, the pregnancy rate is improved compared with the control group, but is far lower than that after the core material of the feeding example 2 is added with corn protein powder and genipin, and in combination with the detection results of the comparative example 1 and the comparative example 2, it can be seen that genipin is not added in the comparative example 2, the effect is close to that of the core material of the comparative example 3, and probably the absorption rate of the corn protein powder is lower; although the geniposide and the corn gluten meal are also added in the comparative example 1, when the addition amount of the geniposide is too large, the effect is reduced compared with that in the example 2, and the dosage of the geniposide is not too high.
Referring to the detection results of the embodiment 2 and the embodiment 4, it can be seen that the embodiment 4 is added with genipin and L-hydroxyproline simultaneously, which is helpful for further improving the pregnancy rate, and probably because the genipin and the L-hydroxyproline can further improve the water solubility of the corn protein powder, so as to promote the absorption of the corn protein powder by the dairy cows; according to the detection result of the embodiment 5, the genipin acid is added after being coated in the embodiment 5, compared with the embodiment 4, the pregnancy rate is further improved, and the genipin acid coating realizes the protection of carboxyl on the side chain of the genipin acid, so that the corn protein powder can be better modified by water dissolution; in combination with the test results of example 6, the pregnancy rates of genipin and L-hydroxyproline were further improved after coating by the coating method of example 6, and in combination with the test data of example 7, it can be seen that when both genipin, L-hydroxyproline and genipin are coated with polyacrylic resin, even though the coating thickness is controlled to be different according to the ratio of the coating to the core material, the pregnancy rate is slightly higher than that of example 4, but is weaker than that of example 6 when the same coating material is used. In combination with the results of the test in example 8, it can be seen that the final pregnancy rate is still reduced when genipin and L-hydroxyproline are coated without calcium carbonate.
In conclusion, it can be seen that when melatonin, corn protein powder and genipin acid are added to the core material, the pregnancy rate of the dairy cows fed with the feed is remarkably improved, and genipin and L-hydroxyproline are further improved; after different coating materials are adopted for the genipin, the genipin and the L-hydroxyproline, the control of the coating thickness is combined, so that the genipin and the L-hydroxyproline are released firstly to act on the corn protein powder, and the genipin is released later to act on the corn protein powder, the protection of the genipin carboxyl is realized, the water-soluble modification effect of the corn protein powder is better, the absorption of a milk cow is more facilitated, melatonin is better assisted, and the pregnancy rate is improved.
The specific embodiments are only for explaining the present application and are not limiting to the present application, and those skilled in the art can make modifications to the embodiments without inventive contribution as required after reading the present specification, but all the embodiments are protected by patent law within the scope of the claims of the present application.
Claims (10)
1. The rumen bypass melatonin composition for improving pregnancy rate is characterized by comprising a core material and an outer coating layer which are sequentially arranged from inside to outside, wherein the core material comprises the following components in percentage by mass: (2-3): (0.5-0.8) melatonin, corn gluten meal and geniposide.
2. The rumen bypass melatonin composition for increasing pregnancy rate according to claim 1, wherein: the core material also comprises genipin and L-hydroxyproline, wherein the addition mass ratio of genipin to genipin acid is 1: (1.2-1.5), wherein the addition mass ratio of the genipin to the L-hydroxyproline is 1: (0.8-1).
3. The rumen bypass melatonin composition for increasing pregnancy rate according to claim 2, wherein: the geniposide is added after being coated by polyacrylic resin, and the addition mass ratio of the polyacrylic resin to the geniposide is 1: (0.5-0.8).
4. The rumen bypass melatonin composition for increasing pregnancy rate according to claim 2, wherein: the genipin and the L-hydroxyproline are added after being coated by a coating agent, and the addition mass ratio of the coating agent to the sum of the genipin and the L-hydroxyproline is 1: (1.5-2).
5. The rumen bypass melatonin composition for increasing pregnancy rate according to claim 4, wherein: the coating agent for coating genipin and L-hydroxyproline comprises the following components in a mass ratio of 1: (2-3): (0.3-0.5): (0.2-0.4) low ester pectin, shellac, and calcium ascorbate, and calcium carbonate.
6. A method for preparing a rumen bypass melatonin composition for increasing pregnancy rate as claimed in claim 1, wherein: the method comprises the following steps:
preparing core material particles: dissolving geniposide in water, adding corn protein powder, mixing and stirring at 35-40 deg.C, adding melatonin, mixing and stirring, granulating, and drying to obtain core material;
coating with an outer coating layer: coating the core material to obtain rumen-passing melatonin composition.
7. The method for preparing a rumen bypass melatonin composition for increasing pregnancy rate as claimed in claim 6, wherein: the core material also comprises genipin and L-hydroxyproline, wherein the addition mass ratio of genipin to genipin acid is 1: (1.2-1.5), wherein the addition mass ratio of genipin to L-hydroxyproline is 1: (0.8-1);
in the step of preparing the core material particles, geniposidic acid is dissolved in water, L-hydroxyproline is added, and then corn protein powder and genipin are added.
8. The method for preparing a rumen bypass melatonin composition for increasing pregnancy rate as claimed in claim 7, wherein: in the core material particle preparation step, the geniposidic acid is added after being coated by polyacrylic resin, and the adding mass ratio of the polyacrylic resin to the geniposidic acid is 1: (0.5-0.8).
9. The method for preparing a rumen bypass melatonin composition for increasing pregnancy rate as claimed in claim 8, wherein: the genipin and the L-hydroxyproline are added after being coated by a coating agent, wherein the coating agent comprises the following components in percentage by mass of 1: (2-3): (0.3-0.5): (0.2-0.4) the mass ratio of the genipin to the L-hydroxyproline to the mass sum of the low-ester pectin, the shellac, the calcium ascorbate and the calcium carbonate is 1 (0.8-1.2);
in the core material particle preparation step, the coating operation of genipin and L-hydroxyproline is as follows:
adding calcium carbonate into the shellac solution, and stirring to obtain a mixture A;
adding genipin and L-hydroxyproline into the mixture A, and stirring to obtain a mixture B;
adding low-ester pectin into the mixture B, heating and stirring to obtain a mixture C;
and (3) dropwise adding the calcium ascorbate solution into the mixture C, stirring at low temperature, drying and forming to obtain the coated genipin/L-hydroxyproline.
10. Use of rumen bypass melatonin composition for increasing pregnancy rate of ruminant in ruminant feed additive is provided.
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