CN111999516A - Sample introduction device and method for testing sample by using quintuplet cup - Google Patents
Sample introduction device and method for testing sample by using quintuplet cup Download PDFInfo
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- CN111999516A CN111999516A CN201910391248.8A CN201910391248A CN111999516A CN 111999516 A CN111999516 A CN 111999516A CN 201910391248 A CN201910391248 A CN 201910391248A CN 111999516 A CN111999516 A CN 111999516A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/026—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having blocks or racks of reaction cells or cuvettes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1079—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices with means for piercing stoppers or septums
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Abstract
The invention discloses a quintuplet cup, a sample feeding device and a detection method using the quintuplet cup, wherein the quintuplet cup comprises a connecting sleeve and a plurality of cup grooves, the connecting sleeve is provided with a plurality of connecting holes corresponding to the cup grooves, and the cup grooves are respectively vertically connected with the connecting holes in a one-to-one correspondence manner, so that the cup grooves are mutually independent; the sample feeding device comprises a sample feeding device body, a quintuplet cup, a ball plunger and a driving device, wherein the ball plunger is connected to the sample feeding device body, and the driving device drives and controls the quintuplet cup to move; the ball plunger is used for limiting the quintuplet cup. All reagents required by detection are integrated in one test card box, so that the use is convenient, and the clinical test time can be saved; simple structure, and the operation is controllable, has reduced the cost simultaneously, is applicable to extensive popularization.
Description
Technical Field
The invention belongs to the technical field of medical equipment, and particularly relates to a quintuplet cup, a sample injection device using the quintuplet cup and a method for testing a sample by using the quintuplet cup.
Background
Chemiluminescence immunoassay combines a chemiluminescence assay technology with high sensitivity and high specificity immune reaction, and is used for detection and analysis technologies of various antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins, medicines and the like. Is a latest immunoassay technology developed after radioimmunoassay, enzyme immunoassay, fluoroimmunoassay and time-resolved fluoroimmunoassay. The chemiluminescence diagnostic system utilizes chemiluminescence to form an excited intermediate through catalysis of a catalyst and oxidation of an oxidant, the excited intermediate simultaneously emits photons (hM) when returning to a stable ground state, and a luminescence signal measuring instrument is utilized to measure the quantum yield of light. The immunoreaction system is to label the luminous matter (generating excited state intermediate under the excitation of reactant) directly on antigen (chemiluminescence immunoassay) or antibody (immunochemiluminescence assay), or enzyme acts on the luminous substrate.
The chemiluminescence diagnosis is widely applied at present, such as detection of infectious diseases, HBsAg, HBsAb, HBeAb and the like; or thyroid function test, TT3, TT4, T3, etc.; or tumor marker detection, AFP, CEA, PSA, NSE, CA50, etc.
Chinese patent document (application number: 201820280185.X) discloses a sample injection device and a system. The sample feeding device comprises a first driving mechanism, a second driving mechanism and a turntable. Through a actuating mechanism drive carousel rotation, place at the storage tank carousel when the reagent pipe and rotate, when the reagent pipe is placed in the storage tank, just can realize rotating and advance the appearance. With this sampling device and sampling needle cooperation use, this kind of mode of rotating to advance a kind makes things convenient for the sampling needle to take a sample more for the straight line sampling mode among the prior art to the structure is compacter. Further, each accommodating groove is driven to rotate by the second driving mechanism, so that the reagent tube placed in the accommodating groove can be driven to rotate, and samples in the reagent tube can be uniformly mixed. However, the sample introduction device places the reaction reagent and the sample to be tested in different reagent tubes, and all the reagent tubes need to be replaced after the test, so that the detection process is complex and the cost is high.
Therefore, there is a need to develop a quintuplet cup with a reasonable design, which integrates the reagents required for detection into one test cartridge and is convenient to use, and a sample introduction device using the quintuplet cup and a method for testing a sample using the quintuplet cup.
Disclosure of Invention
The invention aims to solve the technical problem of providing a quintuplet cup which is reasonable in design, integrates reagents required by detection into a test card box and is convenient to use.
In order to solve the technical problem, the technical scheme adopted by the invention is that the quintuplet cup comprises a connecting sleeve and a plurality of cup grooves, wherein the connecting sleeve is provided with a plurality of connecting holes corresponding to the cup grooves, and the cup grooves are respectively vertically connected with the connecting holes in a one-to-one correspondence manner, so that the cup grooves are mutually independent.
Adopt above-mentioned technical scheme, through setting up a plurality of cup grooves and adopting the adapter sleeve to link together and mutually independent be used for placing the reaction cup and/or preset reaction reagent, realized will detecting required all reagents integration in a test card box, convenient to use just can practice thrift clinical examination time, this quintuplet cup is disposable consumables in the testing process simultaneously, can avoid reagent and sample tube or test card box's repeated washing to use the pollution that probably brings, thereby improve and detect the accuracy.
As a preferable scheme of the invention, the quintuplet cup further comprises a cup sleeve, and the cup sleeve surrounds the outer walls of the plurality of cup grooves and is vertically connected with the connecting sleeve; the connecting sleeve further comprises a plurality of connecting parts, and the connecting parts are respectively arranged among the cup grooves and between the cup grooves and the cup sleeves. The cup grooves are fixed with each other, so that the cup grooves cannot be inclined or misplaced in the detection process.
As a preferable scheme of the invention, one end of the connecting sleeve extends along the horizontal direction to be provided with a handheld part. The handheld part is arranged, so that the quintuplet cup can be conveniently held and is more convenient when placed at the sample inlet.
As a preferable scheme of the present invention, the outer edge of the hand-held portion is arc-shaped, and the back surface of the hand-held portion is provided with at least one anti-slip strip. The edges are arc-shaped and the anti-slip strips are arranged to facilitate the use of the quintuplet cup.
As a preferable scheme of the invention, the quintuplet cup also comprises at least one reaction cup, and the reaction cup is placed in the cup groove. By placing the cuvette in the cup well, a sample or antibody reagent or the like can be placed.
As a preferable scheme of the invention, a clamping groove is arranged on the connecting sleeve at one end of the quintuplet cup, which is far away from the handheld part. The clamping groove is arranged to be matched with the sample feeding device in the testing process.
The invention also aims to solve the problem of providing a sample feeding device matched with a quintuplet cup for integrating all detection reagents.
In order to solve the technical problems, the invention adopts the technical scheme that the sample feeding device comprises a sample feeding device body, a quintuplet cup, a ball plunger and a driving device, wherein the ball plunger is connected to the sample feeding device body, and the driving device drives and controls the quintuplet cup to move; the ball plunger is used for limiting the quintuplet cup. By adopting the technical scheme, the driving device drives the quintuplet cup to move by utilizing the shifting hand device, and the ball head plunger is clamped with the clamping groove of the quintuplet cup, namely the quintuplet cup is in position.
The invention has the further improvement that the sample feeding device also comprises a hand stirring device, wherein the hand stirring device comprises a second hand stirring part, a first hand stirring part and a connecting rod, the first hand stirring part and the second hand stirring part are respectively arranged at two ends of the connecting rod, one side of the first hand stirring part is provided with a hand stirring rotating shaft, the connecting rod is connected with a spring below the first hand stirring part through a spring fixing block, one end of the spring is in press contact with the first hand stirring part, and an optical coupling limiting sheet is connected below the other end of the spring; the driving device drives the quintuplet cup to move through the hand poking device. The driving device drives the quintuplet cup to move by utilizing the first shifting handle and the second shifting handle through the arrangement of the hand pulling device, and meanwhile, the first shifting handle and the second shifting handle have a limiting effect on the quintuplet cup, so that the movement of the quintuplet cup and the position of the quintuplet cup after the movement are better controlled; the arrangement of the light coupling limiting piece can prompt the position of the hand dialing device.
The invention is further improved in that the driving device comprises a fixed block and a guide rail, wherein one side of the fixed block is sleeved, clamped and connected with the guide rail to enable the fixed block to move on the guide rail; the hand pulling device is fixed on the fixed block, and the fixed block drives the hand pulling device to move along the guide rail; the quintuplet cup is placed between the first shifting handle and the second shifting handle and is driven by the first shifting handle and the second shifting handle to move. The fixed block is matched with the guide rail to drive the shifting handle device to move along the guide rail, so that the quintuplet cup moves back and forth along the guide rail, and the movement of the quintuplet cup is favorably controlled better.
The invention is further improved in that the sample feeding device body comprises the first support column, the second support column, a first side baffle and a second side baffle; the first side baffle and the second side baffle are arranged in parallel and are connected between the first supporting column and the second supporting column; the ball plunger is mounted on the first side baffle. The hand shifting device and the quintuplet cup are arranged between the first side baffle and the second side baffle through the arrangement of the first side baffle and the second side baffle, the lower end of the hand shifting device is exposed below the first side baffle and the second side plate and is fixed on the fixing block outside the second side baffle, and the arrangement is further favorable for controlling the movement track of the quintuplet cup and is simultaneously favorable for limiting the ball plunger. The front to back of the position is the moving direction relative to the quintuplet cup, the position where the front end of the quintuplet cup reaches first is front, and the position where the front end of the quintuplet cup reaches back is back.
The invention is further improved in that a touch sensing probe is connected beside the ball plunger through a fixed compression connecting block, and the fixed compression connecting block is arranged on the outer side of the first side baffle and surrounds the ball plunger in the space of the fixed compression connecting block.
As a preferred technical scheme of the present invention, the driving device is installed at the outer side of the second side baffle, the driving device further includes a motor, a screw rod and a slider, the motor is connected with the screw rod, the slider is sleeved on the screw rod, the slider is connected with the fixed block, and the guide rail is installed at the outer side of the second side baffle.
The invention is further improved in that the sample feeding device further comprises a code scanner which is arranged on the outer side of the first side baffle and on one side of the fixed compression connecting block. And the code scanner is arranged for recording the information of the sample to be detected in the five united cups.
The invention is further improved in that the sample feeding device also comprises a guide groove and a sample feeding groove vacancy, wherein the sample feeding groove vacancy is used for placing the pre-discarded quintuplet cup, and the quintuplet cup is driven by a hand pulling device to slide in the guide groove to the sample feeding groove vacancy. After the samples are mixed, the hand pulling device continues to drive the five-linked cup to move, the head of the first hand pulling is tilted to clamp the lower end angle of the handheld part of the five-linked cup under the action of the spring shrinkage and the hand pulling rotating shaft to drive the five-linked cup to move, the five-linked cup slides in the guide groove, and when the empty position of the sample feeding groove is reached, the five-linked cup downwards passes through the waste material groove and is discharged out of the instrument.
The invention is further improved in that the sample introduction device further comprises an optical coupler, wherein the optical coupler is arranged on the first support column through an optical coupler support piece and matched with the optical coupler limiting piece to prompt the position of the hand poking device.
As a preferable technical scheme of the invention, the second side baffle is provided with notches, and the notches are connected through a connecting block.
As a preferred technical scheme, cup grooves of the quintuplet cup are divided into a first cup groove, a second cup groove, a third cup groove, a fourth cup groove and a fifth cup groove, and the clamping groove is positioned between the first cup groove and the second cup groove; the diameters of the second cup groove and the fifth cup groove are larger than those of the first cup groove, the third cup groove and the fourth cup groove, and the second cup groove and the fifth cup groove are used for placing the reaction cup. The quintuplet cup is provided with a clamping groove between the first cup groove and the second cup groove, is clamped with the first spherical plunger and is used for limiting the quintuplet cup; a reagent reaction cup is arranged in a second cup groove in the quintuplet cup, and the reagent reaction cup is designed to be light-proof and is internally provided with a labeled antibody; the reaction cup is placed in the fifth cup groove, and is a sample reaction cup to be tested, and other cup grooves directly load the reagent therein, so that all the reagents required by the detection are integrated into one test card box, the use is convenient, and the clinical test time can be saved; the quintuplet cup is a single consumable, and can effectively avoid possible pollution caused by repeated cleaning and use of the reagent and the sample tube or the test card box, thereby improving the detection accuracy.
The present invention also addresses the problem of providing a method of testing a sample using a quintuplet cup that integrates all of the detection reagents together.
In order to solve the technical problems, the invention adopts the technical scheme that the method for testing the sample by using the quintuplet cup specifically comprises the following steps:
(1) a sample inlet of the detection instrument pops up a quintuplet cup receiving device;
(2) a quintuplet cup is arranged in a receiving device, a sample to be detected is added into a sample reaction cup and placed into a fifth cup groove, a labeled antibody is added into a reagent reaction cup and placed into a second cup groove, a labeled antibody buffer solution is preset in the first cup groove, a reaction buffer solution is preset in the third cup groove, and magnetic beads are preset in the fourth cup groove;
(3) the quintuplet cup enters from a sample inlet of the detection instrument;
(4) firstly, absorbing a labeled antibody buffer solution in a first cup groove by using a combined cleaning needle, adding the labeled antibody buffer solution into a labeled antibody in the reagent reaction cup, and uniformly mixing;
(5) after the combined cleaning needle is cleaned, absorbing the reaction buffer solution in the third cup groove, adding the reaction buffer solution into the fourth cup groove, uniformly mixing the reaction buffer solution with the magnetic beads in the fourth cup groove, and absorbing the uniformly mixed liquid;
(6) adding the uniformly mixed liquid in the step (5) into a sample reaction cup, and uniformly mixing;
(7) then grabbing the reagent reaction cup and the sample reaction cup to be placed in a turntable, and incubating the sample reaction cup;
(8) after the combined cleaning needle is cleaned, sucking liquid in a reagent reaction cup on the turntable, flushing the liquid into a sample reaction cup, uniformly mixing, and then incubating;
(9) the combined washing needle was washed and the sample cuvette was transferred to a dark room, developed and the results read.
By adopting the technical scheme, the detection reagent, the reaction cup filled with the detection sample and the reagent reaction cup marked with the antibody are respectively placed in the independent 5 cup grooves of the quintuplet cup, so that the rapid detection is realized, and the cost is reduced.
Compared with the prior art, the quintuplet cup with a plurality of independent cup grooves is designed, so that the detection reagent, the reaction cup filled with the detection sample and the reagent reaction cup marked with the antibody can be respectively placed in the cup grooves, all reagents required by detection are integrated into one detection cassette, the use is convenient, and the clinical detection time can be saved; the quintuplet cup is a single consumable, so that the pollution possibly caused by repeated cleaning and use of the reagent and the sample tube or the test card box can be effectively avoided, and the detection accuracy is improved; meanwhile, a sample feeding device suitable for the quintuplet cup is designed by matching with the quintuplet cup, and the quintuplet cup is driven to move by a driving device through a hand stirring device. Simple structure, controllable operation, reduced cost and wide application.
Drawings
The following further detailed description of embodiments of the invention is made with reference to the accompanying drawings:
fig. 1 is a schematic perspective view of a quintuplet cup according to embodiment 1 of the present invention;
FIG. 2 is a schematic bottom view of a quintuplet cup according to embodiment 1 of the present invention;
FIG. 3 is a structural view of a reagent cuvette in a second cuvette well in a quintuplet cuvette in example 1 of the present invention;
FIG. 4 is a structural diagram of a sample cuvette in a groove of a fifth cup in a quintuplet cup according to example 1 of the present invention;
FIG. 5 is a schematic perspective view of a sample injection device in example 2 of the present invention;
FIG. 6 is a schematic perspective view of a sample injection device of example 2 of the present invention with a second side baffle removed;
FIG. 7 is a top view structural diagram of a sample injection device in embodiment 2 of the present invention;
fig. 8 is a structural view of a hand dialing member according to embodiment 2 of the invention;
fig. 9 is a side sectional view of the hand dialing member according to embodiment 2 of the invention;
FIG. 10 is an enlarged partial plan view of embodiment 2 of the present invention;
FIG. 11 is a flow chart of a method of testing a sample using a quintuplet cup of the present invention;
wherein: 1-quintuplet cup; 101-a cup groove; 102-a connecting sleeve; 103-connecting hole; 104-a cup sleeve; 105-a connecting portion; 106-a hand-held part; 107-cleats; 108-a reaction cup; 109-card slot; 2-a guide groove; 201-sample groove vacancy position; 3-ball plunger; 4-a drive device; 401-a slider; 402-a guide rail; 403-a motor; 404-a screw rod; 405-fixing block; 5-a hand-dialing device; 501-a second hand-dialing; 502-first paddle; 503-connecting rod; 504-a spring; 505-spring fixing block; 506-hand-pulling rotating shaft; 6-optical coupling limiting sheet; 7-sample introduction device body; 701-a first support column; 702-a second support column; 703-a first side dam; 704-a second side dam; 705-connecting block; 8-fixing the compression connecting block; 9-contact induction probe; 10-a code scanner; 11-an optical coupler; 12-opto-coupler support.
Detailed Description
In order to better explain the technical scheme of the invention, the technical scheme of the invention is further described in detail by combining the drawings and the embodiment.
Example 1: as shown in fig. 1 to 4, the quintuplet cup 1 includes a connecting sleeve 102 and 5 cup grooves 101, the connecting sleeve 102 is provided with 5 connecting holes 103 corresponding to the cup grooves 101, and the 5 cup grooves 101 are respectively vertically connected with the 5 connecting holes 103 in a one-to-one correspondence manner, so that the cup grooves 101 are independent from each other; the quintuplet cup 1 further comprises a cup sleeve 104, and the cup sleeve 104 surrounds the outer walls of the 5 cup grooves 101 and is vertically connected with the connecting sleeve 102; the connecting sleeve 102 further comprises a plurality of connecting parts 105, and the connecting parts 105 are respectively arranged between the 5 cup grooves 101 and between the cup grooves 101 and the cup sleeve 104; one end of the connecting sleeve 102 extends along the horizontal direction to be provided with a handheld part 106; the outer edge of the handheld portion 106 is arc-shaped, and 4 anti-slip strips 107 are arranged on the back surface of the handheld portion 106; the quintuplet cup 1 also comprises 2 reaction cups 108, wherein the reaction cups 108 are placed in the cup groove 101; a clamping groove 109 is arranged on the connecting sleeve 102 at one end of the quintuplet cup 1 far away from the handheld part 106; the cup groove 101 of the quintuplet cup 1 is divided into a first cup groove, a second cup groove, a third cup groove, a fourth cup groove and a fifth cup groove, and the clamping groove 109 is positioned between the first cup groove and the second cup groove; the diameters of the second cup groove and the fifth cup groove are larger than those of the first cup groove, the third cup groove and the fourth cup groove, and the second cup groove and the fifth cup groove are used for placing the reaction cup 108; the five-linked cup 1 is provided with a clamping groove 109 between the first cup groove and the second cup groove, is clamped with the spherical plunger 2 and is used for limiting the five-linked cup 1; a reagent reaction cup is arranged in a second cup groove in the quintuplet cup 1, and the reagent reaction cup is designed to be light-proof and is internally provided with a labeled antibody; the reaction cup 108 is placed in the fifth cup groove, and is a sample reaction cup to be tested, and other cup grooves directly load the reagents therein, so that all the reagents required by detection are integrated into a detection cassette, the use is convenient, and the clinical detection time can be saved; this quintuplet cup 1 is the single consumables, can effectively avoid reagent and sample tube or test card box's repeated cleaning to use the pollution that probably brings to improve detection accuracy.
Example 2: as shown in fig. 5-10, the sample feeding device comprises a sample feeding device body 7, a quintuplet cup 1, a ball plunger 3 and a driving device 4, wherein the ball plunger 3 is connected to the sample feeding device body 7, and the driving device 4 drives and controls the quintuplet cup 1 to move; the ball plunger 3 limits the quintuplet cup 1; the sample feeding device further comprises a hand shifting device 5, wherein the hand shifting device 5 comprises a second hand shifting handle 501, a first hand shifting handle 502 and a connecting rod 503, the second hand shifting handle 501 and the first hand shifting handle 502 are respectively arranged at two ends of the connecting rod 503, and a hand shifting rotating shaft 506 is arranged at one side of the first hand shifting handle 502; a spring 504 is connected to the connecting rod 503 below the first toggle 502 through a spring fixing block 505, one end of the spring 504 is in press-fit contact with the first toggle 502, and an optical coupling limiting sheet 6 is connected below the other end of the spring 504; the driving device 4 drives the quintuplet cup 1 to move through the hand poking device 5; the hand pulling device 5 is arranged, so that the driving device 4 drives the quintuplet cup 1 to move by utilizing the second hand pulling 501 and the first hand pulling 502, and meanwhile, the second hand pulling 501 and the first hand pulling 502 also have a limiting effect on the quintuplet cup 1, so that the movement of the quintuplet cup 1 and the position after the movement are better controlled; the position of the hand dialing device can be prompted by arranging the optocoupler limiting piece 6; the driving device 4 comprises a fixed block 405 and a guide rail 402, wherein one side of the fixed block 405 is sleeved and clamped with the guide rail 402 to enable the fixed block 405 to move on the guide rail 402; the hand-shifting device 5 is fixed on the fixed block 405, and the fixed block 405 drives the hand-shifting device 5 to move along the guide rail 502; the quintuplet cup 1 is placed between the second hand dial 501 and the first hand dial 502, and the quintuplet cup 1 is driven by the second hand dial 501 and the first hand dial 502 to move; the fixed block 405 is matched with the guide rail 402 to drive the hand-shifting device 5 to move along the guide rail 402, so that the quintuplet cup 1 moves back and forth along the guide rail 402, and the movement of the quintuplet cup 1 is favorably controlled better; the sample injection device body 7 comprises the first support column 701, a second support column 702, a first side baffle 703 and a second side baffle 704; the first side baffle 703 and the second side baffle 704 are arranged in parallel and connected between the first support column 701 and the second support column 702; the ball plunger 3 is mounted on the first side baffle 703; the hand poking device 5 and the quintuplet cup 1 are arranged between the first side baffle 703 and the second side baffle 704 through arranging the first side baffle 703 and the second side baffle 704, and the lower end of the hand poking device 5 is exposed below the first side baffle 703 and the second side baffle 704 and is fixed on the fixed block 405 outside the second side baffle 704; a contact induction probe 9 is connected beside the ball plunger 3 through a fixed compression connecting block 8, the fixed compression connecting block 8 is installed on the outer side of the first side baffle 703, and the ball plunger 3 is enclosed in the space of the fixed compression connecting block 8; the driving device 4 is installed on the outer side of the second side baffle 704, the driving device 4 further comprises a motor 403, a screw rod 404 and a sliding block 401, the motor 403 is connected with the screw rod 404, the sliding block 401 is sleeved on the screw rod 404, the sliding block 401 is connected with the fixed block 405, and the guide rail 402 is installed on the outer side of the second side baffle 704; the sampling device further comprises a code scanner 10, wherein the code scanner 10 is installed on the outer side of the first side baffle 703 and on one side of the fixed compression connecting block 8; a code scanner 10 is arranged for recording the information of the sample to be tested in the quintuplet cup 1; the sample introduction device further comprises a guide groove 2 and a sample introduction groove vacancy 201, wherein the sample introduction groove vacancy 201 is used for placing the pre-discarded quintuplet cup 1, and the quintuplet cup 1 is driven by a hand poking device 5 to slide in the guide groove 2 to the sample introduction groove vacancy 201; the sample introduction device further comprises an optical coupler 11, wherein the optical coupler 11 is arranged on the first support column 701 through an optical coupler support part 12, is matched with the optical coupler limiting sheet 6 and is used for prompting the position of the hand poking device 5; the second side baffle 704 is provided with a gap, and the gap is connected through a connecting block 705. The quintuplet cup 1 comprises a connecting sleeve 102 and 5 cup grooves 101, wherein the connecting sleeve 102 is provided with 5 connecting holes 103 corresponding to the cup grooves 101, and the 5 cup grooves 101 are respectively vertically connected with the 5 connecting holes 103 in a one-to-one correspondence manner, so that the cup grooves 101 are mutually independent; the quintuplet cup 1 further comprises a cup sleeve 104, and the cup sleeve 104 surrounds the outer walls of the 5 cup grooves 101 and is vertically connected with the connecting sleeve 102; the connecting sleeve 102 further comprises a plurality of connecting parts 105, and the connecting parts 105 are respectively arranged between the 5 cup grooves 101 and between the cup grooves 101 and the cup sleeve 104; one end of the connecting sleeve 102 extends along the horizontal direction to be provided with a handheld part 106; the outer edge of the handheld part 106 is arc-shaped, and 4 anti-slip strips 107 are arranged on the back surface of the handheld part 106; the quintuplet cup 1 also comprises 2 reaction cups 108, wherein the reaction cups 108 are placed in the cup groove 101; a clamping groove 109 is arranged on the connecting sleeve 102 at one end of the quintuplet cup 1 far away from the handheld part 106; the cup groove 101 of the quintuplet cup 1 is divided into a first cup groove, a second cup groove, a third cup groove, a fourth cup groove and a fifth cup groove, and the clamping groove 109 is positioned between the first cup groove and the second cup groove; the diameters of the second cup groove and the fifth cup groove are larger than those of the first cup groove, the third cup groove and the fourth cup groove, and the second cup groove and the fifth cup groove are used for placing the reaction cup 108; the five-linked cup 1 is provided with a clamping groove 109 between the first cup groove and the second cup groove, is clamped with the spherical plunger 3 and is used for limiting the five-linked cup 1; a reagent reaction cup is arranged in a second cup groove in the quintuplet cup 1, and the reagent reaction cup is designed to be light-proof and is internally provided with a labeled antibody; the reaction cup 108 is placed in the fifth cup groove, and is a sample reaction cup to be tested, and other cup grooves directly load the reagents therein, so that all the reagents required by detection are integrated into a detection cassette, the use is convenient, and the clinical detection time can be saved; this quintuplet cup 1 is the single consumables, can effectively avoid the repeated washing of sample seat to use the pollution that probably brings to improve and detect the accuracy.
Wherein the sampling process of the sampling device is as follows: firstly, placing a reagent reaction cup in a second cup groove in a quintuplet cup 1, wherein a labeled antibody is filled in the reagent reaction cup; a reaction cup is placed in the fifth cup groove and is a sample reaction cup to be tested, and reagents are directly loaded in other cup grooves; scanning the five-link cup 1 by a code scanner 10, inputting information of a sample to be detected, then placing the five-link cup 1 into a sample inlet of a detection instrument, starting a motor 403, driving a sliding block 401 to move by the motor 403 through a lead screw 404, driving a fixed block 405 to move on a guide rail 402 by the sliding block 401, and synchronously driving a hand-dialing device 5 connected with the fixed block 405 to move along the guide rail 402 so as to drive the five-link cup 1 to move together; when the front end of the quintuplet cup 1 reaches the spherical plunger 3, the clamping groove 109 at the front end of the quintuplet cup 1 is clamped with the spherical plunger 3, and the manual putting position is prompted to be reached; the contact induction probe 9 senses that the quintuplet cup 1 is in place; at the moment, the first shifting hand 502 is located at the initial position, the second shifting hand 501 contacts the front end of the quintuplet cup 1 in the corner of the first supporting column 701, and the display screen of the detection instrument prompts that the next operation can be carried out; after the labeled antibody, the buffer solution, the sample, the magnetic beads and the like in the quintuplet cup 1 are mixed, transferring the sample reaction cup and the labeled antibody cup to an incubation disc; the detecting instrument controls the quintuplet cup 1 to be shifted by the hand shifting device 5 to move forwards continuously, the head of the first shifting hand 502 tilts under the action of the contraction of the spring 504 and the action of the shifting hand rotating shaft 506 to clamp the lower end angle of the handheld part 106 of the quintuplet cup 1 to drive the quintuplet cup 1 to move, the quintuplet cup 1 slides in the guide groove 2, and when the position reaches the vacant position 201 of the sample feeding groove, the detecting instrument is discharged out of the detecting instrument through the waste material groove downwards; after the quintuplet cup 1 is discarded, the motor 403 moves to drive the hand-shifting device 5 to move forwards, the optical coupler 11 on the first supporting column 701 is matched with the optical coupler limiting piece 6 fixedly connected with the first hand-shifting 502, the hand-shifting device 5 is prompted to reach the initial position, and the quintuplet cup 1 can be fed again.
As shown in fig. 11, the method for testing a sample by using a quintuplet cup specifically comprises the following steps:
(1) a sample inlet of the detection instrument pops up a quintuplet cup receiving device;
(2) the method comprises the following steps of (1) loading a quintuplet cup 1 in a receiving device, adding a sample to be detected into a sample reaction cup, placing the sample reaction cup into a fifth cup groove, adding a labeled antibody into a reagent reaction cup, placing the reagent reaction cup into a second cup groove, presetting labeled antibody buffer solution in the first cup groove, presetting reaction buffer solution in the third cup groove, and presetting magnetic beads in the fourth cup groove;
(3) the quintuplet cup 1 enters from a sample inlet of the detection instrument;
(4) firstly, absorbing a labeled antibody buffer solution in a first cup groove by using a combined cleaning needle, adding the labeled antibody buffer solution into a labeled antibody in the reagent reaction cup, and uniformly mixing;
(5) after the combined cleaning needle is cleaned, absorbing the reaction buffer solution in the third cup groove, adding the reaction buffer solution into the fourth cup groove, uniformly mixing the reaction buffer solution with the magnetic beads in the fourth cup groove, and absorbing the uniformly mixed liquid;
(6) adding the uniformly mixed liquid in the step (5) into a sample reaction cup, and uniformly mixing;
(7) then grabbing the reagent reaction cup and the sample reaction cup to be placed in a turntable, and incubating the sample reaction cup;
(8) after the combined cleaning needle is cleaned, sucking liquid in a reagent reaction cup on the turntable, flushing the liquid into a sample reaction cup, uniformly mixing, and then incubating;
(9) the combined washing needle was washed and the sample cuvette was transferred to a dark room, developed and the results read.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the present invention, and that various changes and modifications, such as changes in the shape or size of the quintuplet cup or other minor adjustments, may be made without departing from the spirit and scope of the invention and these changes and modifications are within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (18)
1. The utility model provides a quintuplet cup, its characterized in that, this quintuplet cup includes adapter sleeve and a plurality of cup groove, be equipped with a plurality of on the adapter sleeve with the connecting hole that the cup groove corresponds, it is a plurality of the cup groove respectively with a plurality of the connecting hole one-to-one is connected perpendicularly to make a plurality of the cup groove is mutually independent.
2. The quintuplet cup as claimed in claim 1, further comprising a sleeve vertically connected to the connection sleeve around the outer wall of the plurality of cup grooves; the connecting sleeve further comprises a plurality of connecting parts, and the connecting parts are respectively arranged among the cup grooves and between the cup grooves and the cup sleeves.
3. The quintuplet cup as claimed in claim 1, wherein one end of the connecting sleeve is provided with a hand-held portion extending in a horizontal direction.
4. The quintuplet cup of claim 3, wherein an outer edge of the hand-held portion is arcuate, and a back surface of the hand-held portion is provided with at least one cleat.
5. The quintuplet cup of claim 1, further comprising at least one reaction cup, wherein the reaction cup is placed in the cup well.
6. The quintuplet cup of claim 3, wherein a clamping groove is formed in the connecting sleeve at one end of the quintuplet cup, which is far away from the handheld portion.
7. A sample introduction device using the quintuplet cup of claims 1-6, comprising a sample introduction device body, the quintuplet cup, a ball plunger and a driving device, wherein the ball plunger is connected to the sample introduction device body, and the driving device drives and controls the quintuplet cup to move; the ball plunger is used for limiting the quintuplet cup.
8. The sample feeding device according to claim 7, further comprising a hand pulling device, wherein the hand pulling device comprises a first hand pulling device, a second hand pulling device and a connecting rod, the first hand pulling device and the second hand pulling device are respectively arranged at two ends of the connecting rod, a hand pulling rotating shaft is arranged at one side of the first hand pulling device, the connecting rod is connected with a spring through a spring fixing block below the first hand pulling device, one end of the spring is in press contact with the first hand pulling device, and an optical coupling limiting sheet is connected below the other end of the spring; the driving device drives the quintuplet cup to move through the hand poking device.
9. The sample feeding device as claimed in claim 8, wherein the driving device comprises a fixed block and a guide rail, and one side of the fixed block is sleeved and clamped with the guide rail to move the fixed block on the guide rail; the hand pulling device is fixed on the fixed block, and the fixed block drives the hand pulling device to move along the guide rail; the quintuplet cup is placed between the first shifting handle and the second shifting handle and is driven by the first shifting handle and the second shifting handle to move.
10. The sample introduction device according to claim 9, wherein the sample introduction device body comprises the first support column, a second support column, a first side baffle, and a second side baffle; the first side baffle and the second side baffle are arranged in parallel and are connected between the first supporting column and the second supporting column; the ball plunger is mounted on the first side baffle.
11. The sampling device of claim 10, wherein a contact sensing probe is connected beside the ball plunger through a fixed compression connecting block, and the fixed compression connecting block is installed outside the first side baffle and encloses the ball plunger in a space of the fixed compression connecting block.
12. The sampling device according to claim 10, wherein the driving device is installed outside the second side baffle, the driving device further comprises a motor, a screw rod and a slider, the motor is connected with the screw rod, the slider is sleeved on the screw rod, the slider is connected with the fixed block, and the guide rail is installed outside the second side baffle.
13. The sample introduction device according to claim 11, further comprising a code scanner mounted outside the first side baffle and mounted on one side of the fixed compression connection block.
14. The sample introduction device according to claim 11, further comprising a guide slot and a sample introduction slot vacancy, wherein the sample introduction slot vacancy is used for placing the pre-discarded quintuplet cup, and the quintuplet cup is driven by a hand poking device to slide in the guide slot to the sample introduction slot vacancy.
15. The sampling device of claim 11, further comprising an optical coupler, wherein the optical coupler is disposed on the first support column through an optical coupler support and is matched with the optical coupler limiting piece to prompt the position of the hand-shifting device.
16. The sample injection device as claimed in claim 11, wherein the second side baffle is provided with a gap, and the gap is connected through a connecting block.
17. The sample introduction device according to claim 11, wherein the cup grooves of the quintuplet cup are divided into a first cup groove, a second cup groove, a third cup groove, a fourth cup groove and a fifth cup groove, and the clamping groove is located between the first cup groove and the second cup groove; the diameters of the second cup groove and the fifth cup groove are larger than those of the first cup groove, the third cup groove and the fourth cup groove, and the second cup groove and the fifth cup groove are used for placing the reaction cup.
18. A method for testing a sample by using a quintuplet cup is characterized by comprising the following steps:
(1) a sample inlet of the detection instrument pops up a quintuplet cup receiving device;
(2) a quintuplet cup is arranged in a receiving device, a sample to be detected is added into a sample reaction cup and placed into a fifth cup groove, a labeled antibody is added into a reagent reaction cup and placed into a second cup groove, a labeled antibody buffer solution is preset in the first cup groove, a reaction buffer solution is preset in the third cup groove, and magnetic beads are preset in the fourth cup groove;
(3) the quintuplet cup enters from a sample inlet of the detection instrument;
(4) firstly, absorbing a labeled antibody buffer solution in a first cup groove by using a combined cleaning needle, adding the labeled antibody buffer solution into a labeled antibody in the reagent reaction cup, and uniformly mixing;
(5) after the combined cleaning needle is cleaned, absorbing the reaction buffer solution in the third cup groove, adding the reaction buffer solution into the fourth cup groove, uniformly mixing the reaction buffer solution with the magnetic beads in the fourth cup groove, and absorbing the uniformly mixed liquid;
(6) adding the uniformly mixed liquid in the step (5) into a sample reaction cup, and uniformly mixing;
(7) then grabbing the reagent reaction cup and the sample reaction cup to be placed in a turntable, and incubating the sample reaction cup;
(8) after the combined cleaning needle is cleaned, sucking liquid in a reagent reaction cup on the turntable, flushing the liquid into a sample reaction cup, uniformly mixing, and then incubating;
(9) the combined washing needle was washed and the sample cuvette was transferred to a dark room, developed and the results read.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910391248.8A CN111999516B (en) | 2019-05-11 | 2019-05-11 | Sample injection device and method for testing sample by using five-joint cup |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910391248.8A CN111999516B (en) | 2019-05-11 | 2019-05-11 | Sample injection device and method for testing sample by using five-joint cup |
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| CN111999516A true CN111999516A (en) | 2020-11-27 |
| CN111999516B CN111999516B (en) | 2024-10-18 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108107227A (en) * | 2018-02-09 | 2018-06-01 | 深圳市活水床旁诊断仪器有限公司 | A kind of chemiluminescence immune assay chip cartridges and operating method |
| CN108593949A (en) * | 2018-02-11 | 2018-09-28 | 金博特（北京）生物科技有限公司 | A kind of chemical illumination immunity analysis instrument |
| CN210051779U (en) * | 2019-05-11 | 2020-02-11 | 南京岚煜生物科技有限公司 | Quintuplet cup and sample introduction device using same |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108107227A (en) * | 2018-02-09 | 2018-06-01 | 深圳市活水床旁诊断仪器有限公司 | A kind of chemiluminescence immune assay chip cartridges and operating method |
| CN108593949A (en) * | 2018-02-11 | 2018-09-28 | 金博特（北京）生物科技有限公司 | A kind of chemical illumination immunity analysis instrument |
| CN210051779U (en) * | 2019-05-11 | 2020-02-11 | 南京岚煜生物科技有限公司 | Quintuplet cup and sample introduction device using same |
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