CN117990897A - Biochemical immunity all-in-one - Google Patents

Abstract

A biochemical immune all-in-one machine, comprising: the device comprises a rack, a reagent processing module, a scanning module, a sample processing module, an incubation module, a pipetting device, a cleaning device module, a mixing reading module, a three-dimensional arm module, a substrate refrigerating assembly, a reaction module, a TIP head module, a biochemical detection module, a waste box module, a cleaning liquid and waste liquid barrel, an electric control part and a computer control end. The device can detect the same or different projects simultaneously, has large detection capacity, fully automatic detection process, effectively improves detection efficiency, and has simple integral structure, small occupied area and high economic benefit; the sample, the reaction cup, the TIP head and most of equipment devices are shared in the biochemical detection and the immune detection processes, the equipment integration level is high, the unification that a plurality of items and consumable materials can be detected by the same tube sample is realized, and the sample detection amount and detection time are reduced.

Description

Biochemical immunity all-in-one

Technical Field

The invention relates to the technical field of in-vitro diagnosis and detection, in particular to a biochemical immunity integrated machine.

Background

Biochemical analyzers and immunoassays are two types of analyzers commonly used in the field of in vitro diagnosis. The biochemical analyzer is an instrument for measuring a specific chemical component in body fluid by adopting a photoelectric colorimetric principle, is mainly used for quantitative measurement of biochemical and chemical components such as hemoglobin, cholesterol, triglyceride, albumin, C-reactive protein, glutamate aminotransferase, glucose, potassium, sodium, calcium and the like in clinical blood and other body fluid samples, and is one of the most commonly used inspection instruments for clinical analysis. The immunity analyzer is a high-selectivity diagnosis instrument which is established by utilizing the specific reaction between the substance to be detected and the corresponding substance, and usually realizes detection through the immune reaction of antigen and antibody, and the sensitivity of detection is enhanced by utilizing various carriers to amplify reaction signals (the markers are isotopes, enzymes and chemiluminescent substances), including radioimmunoassay, enzyme-linked immunoassay, chemiluminescence and the like, and is mainly used for detecting in vivo micromolecular proteins, hormones, fatty acids, medicines and other substances, and is usually used for detecting protein targets such as infectious diseases, endocrine diseases, tumors, pregnancy tests, blood group antigens and the like.

At present, biochemical detection and luminous immunity detection are carried out on two instruments separately, or a large-scale assembly line type equipment biochemical immunity integrated machine, in clinical practice, biochemical detection and immunity detection are often needed to be carried out on a patient at the same time, and at present, common practice is to send blood samples of the patient to the instruments of biochemical analysis and immunity analysis respectively for detection, or to be equipped with the large-scale assembly line type equipment biochemical immunity integrated machine for detection.

Based on the prior art, when detecting on two instruments, two large-scale devices are needed, the blood sampling amount is large, more pains are brought to patients, too much blood sampling is very unfavorable to the patients for patients with bad physical states, and meanwhile, the detection takes more time to influence the detection efficiency; the large-scale assembly line type biochemical immunity integrated machine is complex in structure, large in occupied area, high in price of equipment and high in detection corresponding project charge, and is inconvenient for primary hospitals or physical examination institutions to develop project detection.

Disclosure of Invention

The invention mainly solves the technical problem of how to detect the same or different items at the same time.

According to a first aspect, in one embodiment, there is provided a biochemical immune integrated machine, including: the device comprises a rack, a reagent processing module, a scanning module, a sample processing module, an incubation module, a pipetting device, a cleaning device module, a mixing reading module, a three-dimensional arm module, a substrate refrigerating assembly, a reaction module, a TIP head module, a biochemical detection module and a waste box module, a cleaning liquid and a waste liquid barrel which are arranged on the outer side of the rack;

The sample processing module is provided with a sample rack for arranging a plurality of sample tubes; the scanning module is used for reading the test strip information of the sample; the incubation module is used for placing the reaction cup and providing an incubation reaction environment for the reagent in the reaction cup; the pipetting device is used for sucking, transferring and uniformly mixing reagents or samples;

The cleaning device module is used for cleaning magnetic beads; the mixing reading module is used for vibrating the uniform reaction cup, detecting the luminescence value of the reaction and outputting the luminescence value to a computer end for result conversion; the three-dimensional arm module is used for transferring a reagent, a sample or a reaction cup; the substrate refrigeration assembly is used for keeping the constant temperature of the reagent; the pipetting device is arranged on the three-dimensional arm module and is used for sleeving the reaction cup; the reaction module is used for testing the reaction of each reagent and sample in the reaction process; the reagent processing module is used for refrigerating, storing and uniformly mixing the reagents; the TIP head module is used for placing a TIP head; the biochemical detection module is used for biochemical test; the waste box module is used for recovering the reaction cup and the TIP head which are tested; the cleaning liquid and the waste liquid barrel are used for placing the cleaning liquid and the waste liquid.

In another embodiment, the incubation module comprises a support frame, wen Yo arranged on the support frame, a cup holder and a temperature control assembly, wherein the cup holder is arranged on the incubation seat, and a plurality of mounting positions are arranged on the cup holder and used for bearing a reaction cup; the incubation seat is arranged on the temperature control component, and the temperature control component is used for heating the incubation seat.

In another embodiment, the temperature control assembly comprises a heating film, a temperature sensor and a temperature control switch, wherein the heating film is arranged at the bottom of the incubation seat, the temperature control switch and the temperature sensor are arranged on one side of the heating film, the temperature sensor is used for measuring and transmitting the ambient temperature of the reaction in real time, the heating film is used for heating the incubation seat, and the temperature control switch is used for powering off at a fixed temperature to ensure the temperature environment required by the reaction of the reagent.

In another embodiment, the biochemical detection module is disposed on the incubation seat and comprises a plurality of detection seats and a photoreaction assembly disposed on the detection seats, the detection seats are used for placing the reaction cups for reagent detection, the photoreaction assembly is used for forming a photodetection channel, the photodetection channel passes through the reaction cups for detecting project parameters of the same wavelength, and the photodetection channel is disposed perpendicular to the reaction cups.

In another embodiment, the photoreaction assembly includes a light emitting portion for emitting a light source through the cuvette and a light receiving portion for receiving a light wave passing through the cuvette and capable of converting the intensity of the light wave into an electrical signal to calculate the concentration of the measured substance.

In another embodiment, any one of the detecting seats is provided with a plurality of groups of light reaction components, the plurality of groups of light emission parts are used for emitting light waves with different wavelengths, and the centers of the plurality of light detection channels are collected at the bottom of the reaction cup so as to meet different project tests according to different measured wavelengths.

In another embodiment, the photoreaction assembly further includes a condensing part disposed at one side of the light receiving part and configured to condense light waves transmitted through the reaction cup.

In another embodiment, a light filtering part is provided at one side of the light receiving part, and the light filtering part is used for filtering the stray light.

In another embodiment, the detection seat is provided with a multi-surface column structure, the top wall of the detection seat is provided with detection placement holes for placing the reaction cups, the side surfaces of the detection seat are provided with two numbers, the light emitting parts and the light receiving parts are distributed on the two side surfaces of the detection seat, which are symmetrically arranged, the detection seat is correspondingly provided with a plurality of light path holes for light waves to pass through, and the light path holes are communicated with the detection placement holes.

In another embodiment, the reaction cup comprises a large-diameter section and a small-diameter section from top to bottom, wherein the diameter of the large-diameter section is larger than that of the detection placement hole and is used for limiting the top wall of the detection seat, and the small-diameter section extends into the detection placement hole and is positioned at the center of intersection of a plurality of light path holes; the light emitting part, the light path hole and the light receiving part are arranged along the same central line and are perpendicular to the axis of the small-diameter section.

According to the biochemical immune integrated machine of the embodiment, a sample tube loaded with items to be detected is manually clamped on a sample frame and then integrally inserted into a position corresponding to a sample processing module, meanwhile, a scanning module synchronously scans information on the sample tube, then a reagent box is placed at a reagent box bearing position in an instrument, a TIP head is placed on a TIP head module, a reaction cup is placed on an incubation module, a cabin door is closed, and the items can be selected to start testing; when the detection is carried out, the sample processing module is provided with a plurality of sample positions, and single or a plurality of samples can be tested simultaneously; the test items can be selected into single or a plurality of different combination items, and the test samples and the item time are ordered according to the test flow software of each item; the biochemical and immunological integrated machine provided by the application completes biochemical detection and immunological detection on the same equipment, and has the advantages of more accurate and efficient detection, higher integration degree, more compact structure and smaller volume.

Drawings

FIG. 1 is a schematic diagram of the main structure of a biochemical immune system in an embodiment;

FIG. 2 is a flow chart of test operations in one embodiment;

FIG. 3 is a schematic structural view of a reaction cup according to an embodiment;

FIG. 4 is a schematic perspective view of an incubation test module according to one embodiment;

FIG. 5 is a schematic diagram of a biochemical detection module according to an embodiment;

FIG. 6 is an internal anatomic view of a biochemical detection module in one embodiment.

Reference numerals: 1. a frame; 2. a sample processing module; 3. an incubation module; 301. a support frame; 302. an incubation seat; 303. cup holder; 304. heating the film; 305. a temperature sensor; 306. a temperature control switch; 4. a cleaning device module; 5. a blending reading module; 6. a scanning module; 7. a waste cartridge module; 8. a three-dimensional arm module; 9. a substrate refrigeration assembly; 10. a pipetting device; 11. a reaction module; 12. cleaning liquid and waste liquid barrels; 13. a biochemical detection module; 131. a detection seat; 1311. detecting a placement hole; 132. a light emitting section; 1321. a light source; 133. a light receiving section; 1331. a receiving chip; 134. a light-gathering section; 135. a light filtering part; 14. a reagent processing module; 15. a TIP head module; 16. a reaction cup; 161. a large diameter section; 162. and a small diameter section.

Detailed Description

The application will be described in further detail below with reference to the drawings by means of specific embodiments. Wherein like elements in different embodiments are numbered alike in association. In the following embodiments, numerous specific details are set forth in order to provide a better understanding of the present application. However, one skilled in the art will readily recognize that some of the features may be omitted, or replaced by other elements, materials, or methods in different situations. In some instances, related operations of the present application have not been shown or described in the specification in order to avoid obscuring the core portions of the present application, and may be unnecessary to persons skilled in the art from a detailed description of the related operations, which may be presented in the description and general knowledge of one skilled in the art.

Furthermore, the described features, operations, or characteristics of the description may be combined in any suitable manner in various embodiments. Also, various steps or acts in the method descriptions may be interchanged or modified in a manner apparent to those of ordinary skill in the art. Thus, the various orders in the description and drawings are for clarity of description of only certain embodiments, and are not meant to be required orders unless otherwise indicated.

The numbering of the components itself, e.g. "first", "second", etc., is used herein merely to distinguish between the described objects and does not have any sequential or technical meaning. The term "coupled" as used herein includes both direct and indirect coupling (coupling), unless otherwise indicated.

The biochemical analyzer and the immunity analyzer are two kinds of analysis instruments commonly used in the field of in-vitro diagnosis, at present, biochemical detection and luminous immunity detection are carried out on the two kinds of instruments separately, or a large-scale assembly line type equipment biochemical immunity integrated machine, in clinical practice, a patient always needs to carry out biochemical detection and immunity detection at the same time, at present, the common practice is to send blood samples of the patient to the biochemical analysis and immunity analysis instruments respectively for detection, two large-scale equipment are needed, and meanwhile, the blood sampling amount is too much, more pain is brought to the patient, too much blood sampling is very unfavorable to the patient, and more time is spent for detection at the same time, so that the detection efficiency is affected; or be equipped with large-scale pipelined equipment biochemical immunity all-in-one and detect, but large-scale pipelined equipment biochemical immunity all-in-one structure is complicated, and area is big, and equipment price is expensive and detect corresponding project charge is high, is inconvenient for basic unit's hospital or physical examination institution to develop project detection.

In the embodiment of the invention, the biochemical analysis and immune analysis devices are integrated and arranged on one device, and the device is provided with a shared rack 1, a sample processing module 2, a reagent processing module 14, an incubation module 3, a three-dimensional arm module 8, a pipetting device 10, a scanning module 6, a TIP head module 15 and a waste box module 7, so that biochemical detection and immune detection can be completed on the same device, the integration degree is higher, the structure is more compact, and the volume is smaller.

Referring to fig. 1, in one embodiment, a biochemical immune integrated machine is provided, including: the device comprises a rack 1, a reagent processing module 14, a scanning module 6, a sample processing module 2, an incubation module 3, a pipetting device 10, a cleaning device module 4, a mixing read value module 5, a three-dimensional arm module 8, a substrate refrigeration assembly 9, a reaction module 11, a TIP head module 15, a biochemical detection module 13, a waste box module 7, a cleaning liquid and waste liquid barrel 12, an electric control part and a computer control end. The sample processing module 2 is provided with a sample rack for arranging a plurality of sample tubes; the scanning module 6 is used for reading the test strip information of the sample; the incubation module 3 is used for placing the reaction cup 16 and providing an incubation reaction environment for the reagent in the reaction cup 16; the pipetting device 10 is used for aspirating, transferring, mixing reagents or samples; the cleaning device module 4 is used for cleaning magnetic beads; the mixing read value module 5 is used for vibrating the uniform reaction cup 16, detecting the luminescence value of the reaction and outputting the luminescence value to a computer end for result conversion; the three-dimensional arm module 8 is used to transfer reagents, samples or cuvettes 16; the substrate refrigeration assembly 9 is used for keeping the constant temperature of the reagent; the pipetting device 10 is arranged on the three-dimensional arm module 8 and is used for sleeving the reaction cup 16; the reaction module 11 is used for testing the reaction of each reagent and sample in the reaction process; a reagent processing module 14 for refrigerating, storing and mixing the reagent; the TIP head module 15 is used for placing a TIP head; the biochemical detection module 13 is used for biochemical test; the waste box module 7 is used for retracting the reaction cup 16 and the TIP head after the test; the cleaning liquid and waste liquid barrel 12 is used for placing cleaning liquid and waste liquid; the electronic control part and the computer control end are used for controlling the testing steps of each module, so that the whole biochemical immune integrated machine is automated.

In the embodiment of the application, the sample processing module 2 is provided with thirty sample positions, and can test single or multiple samples simultaneously.

When the biochemical immunity integrated machine is used for detection, the test operation flow is as follows:

selecting items to be tested on a screen of the rack 1, wherein the test items can be selected into single or multiple items with different combinations, sequencing each test sample and item time according to test flow software of each item, and simultaneously testing single or multiple samples, wherein the positions of each test sample have no interference effect;

Manually clamping a sample tube loaded with items to be detected on a sample frame, then integrally inserting the sample tube into a position corresponding to a sample processing module 2, and synchronously scanning information on the sample tube through a scanning module 6 while inserting the sample frame;

the reagent box is placed at a reagent box bearing position in the instrument, the TIP head is placed on the TIP head module 15, the reaction cup 16 is placed on the incubation module 3, the cabin door is closed, and the test is started by clicking a selected item;

The testing process is fully automatic;

If a luminescent immunoassay is performed, the test procedure includes: sample frame test information code scanning and reading, automatic TIP head taking, reagent and sample sucking to the reaction cup 16, reagent incubation, reagent mixing (mixing in a sucking and spitting mode), magnetic particle cleaning and separation, substrate adding, reaction cup 16 sleeving by the pipetting device 10 to the mixing reading module 5 for vibration mixing and luminous value reading, test result outputting, and discarding the tested reaction cup 16 and TIP head to a waste box.

If a biochemical test is performed, the test procedure includes: sample rack test information code scanning and reading, automatic TIP head taking, reagent and sample sucking to the reaction cup 16, reagent mixing (mixing in sucking and spitting mode), reagent transferring device 10 sleeve the reaction cup 16 to the biochemical detection module 13 for detection and reading, test result outputting, and discarding the tested reaction cup 16 and TIP head to a waste box.

Referring to fig. 1, in the embodiment of the present application, a full-automatic biochemical immune integrated machine may test a single sample or multiple samples simultaneously by using a single test method of luminescence immune test and biochemical test or one of them, and test items may select a single or multiple items with different combinations, and the test samples and item times may be ordered according to the test flow software of each item. Specifically, the rack 1, the sample processing module 2, the reagent processing module 14, the incubation module 3, the three-dimensional arm module 8, the pipetting device 10, the scanning module 6, the TIP head module 15 and the waste cartridge module 7 are shared in the biochemical detection and the immunoluminescent detection processes.

In the embodiment of the present application, the cleaning device module 4, the pipetting device 10, the reaction module 11, the scanning module 6, the reagent processing module 14, the TIP head module 15, and the electronic control part and the computer control end may be implemented in any feasible manner in the prior art to achieve the functions of the modules,

The sample processing module 2 comprises a sensor arranged at the mounting position of the sample rack, and when the sample pipe is placed on the sample rack, the sample rack is inserted into the sample processing module 2, the sensor is used for detecting an insertion signal and transmitting the insertion signal to a computer control end, so that an instrument can master sample loading information at any time.

The reagent processing module 14 comprises a reagent kit frame, wherein the reagent kit frame contains a reagent bottle and a magnetic bead bottle, the magnetic bead bottle needs to rotate continuously to ensure that magnetic beads in the bottle are not precipitated when in use, and the reagent processing module 14 also comprises a structure for driving the magnetic bead bottle to rotate so as to ensure that the magnetic beads are uniformly mixed.

The reaction module 11 and the cleaning device module 4 are arranged, the reaction cup 16 is grabbed by the grabbing cup clamping sleeve and then rapidly rotated, and the sample, the reagent and the magnetic beads in the reaction cup 16 are driven to be uniformly mixed by a structure of controlling the rotation by software; after being evenly mixed, the magnetic separation can be better carried out, and the waste liquid is pumped away and discharged after being cleaned by the cleaning liquid.

After the special reagent for detection items is added into the reaction cup 16 by other modules in the whole chemiluminescent process and the magnetic beads are added and mixed uniformly, the reaction cup 16 is grabbed by the three-dimensional arm module 8 and put into a detection position for luminescent immunodetection, then the value of the luminescent value of the reagent in the reaction cup 16 is directly read, the luminescent value of the reagent is detected and output to a PC end for result conversion, and then the detection result is output, so that the luminescent immunodetection can be completed, and the detected reaction cup 16 can be put into the waste box module 7 for processing.

The TIP head module 15 comprises a TIP head box for loading a TIP head, wherein the TIP head comprises a TIP head upper pipe, a TIP head lower pipe and a suction head part, the inner size of a nozzle of the TIP head is consistent with that of a nozzle of the reaction cup 16, the size can be matched with the pressure head of the front section of the pipetting device 10, and the pipetting device 10 can be directly pressed into the pipette device for insertion.

The waste cartridge module 7 comprises waste cartridges. The waste cartridge can load the used TIP head and the reaction cup 16, the waste cartridge module 7 further comprises a structure capable of driving the waste cartridge to move and shake the TIP head and the reaction cup 16 uniformly, and in this embodiment, the waste cartridge is preferably made of aluminum alloy.

The three-dimensional arm module 8 is used for driving the pipetting device 10 to move, and comprises a X, Y, Z axial movement structure, and by arranging a Y-axis movement assembly on the X-axis movement assembly and arranging a Z-axis movement assembly on the Y-axis movement assembly, an executive component arranged on the Z-axis movement assembly, namely the pipetting device 10, can complete the movement in the front-back, left-right and up-down directions in space to sleeve the TIP head and the reaction cup 16, the TIP head is the same as the inner caliber size at the upper end of the reaction cup 16, the pipetting device 10 is convenient to sleeve the TIP head to absorb liquid and automatically withdraw, and the aim of sleeve the reaction cup 16 to transfer and withdraw a designated station can be realized.

Referring to fig. 1, a reagent processing module 14, a scanning module 6, a sample processing module 2, an incubation module 3, a pipetting device 10, a cleaning device module 4, a mixing read module 5, a three-dimensional arm module 8, a substrate refrigeration assembly 9, a reaction module 11, a TIP head module 15, and a biochemical detection module 13 are all disposed in a rack 1, and a waste box module 7, a cleaning solution and a waste liquid barrel 12 are disposed outside the rack 1, so that the structure of the detection step is more concentrated. Specifically, since the steps and methods of biochemical detection and luminescence immunoassay belong to the existing schemes, that is, the structure and test flow of each module can refer to the disclosure of the authorized prior art, so as to achieve the above objective.

In the embodiment of the present application, please refer to fig. 1, when performing biochemical detection, the reaction cup 16 moves to the biochemical detection module 13 for testing after the incubation module 3 completes incubation, so when the orientation is set, the incubation module 3 is set adjacent to the biochemical detection module 13; specifically, incubation module 3 includes support frame 301, wen Yo on support frame 301, cup holder 303 and control by temperature change subassembly, and support frame 301 includes four spinal branch daggers, and the support column lower extreme is fixed in frame 1, and the upper end is used for supporting and installing incubation seat 302's corner, and cup holder 303 installs on incubation seat 302, in this embodiment, is provided with seventy installation positions on cup holder 303 for bear reaction cup 16, and biochemical detection module 13 sets up in cup holder 303 one side, and the control by temperature change subassembly is used for heating incubation seat 302. The reaction cup 16 and the TIP head used in the luminescence immunoassay and biochemical test project are common, and the unification of consumable materials is realized.

Referring to fig. 1, the temperature control assembly includes a heating film 304, a temperature sensor 305 and a temperature control switch 306, wherein the heating film 304 is disposed at the bottom of the incubation seat 302, the temperature control switch 306 and the temperature sensor 305 are disposed on the side surfaces of the heating film 304 and are fixed on a bar connected to the tops of the two support columns, the temperature sensor 305 is used for measuring, transmitting and displaying the current reaction environment temperature in real time, the heating film 304 is used for heating the incubation seat 302, ensuring the temperature environment required by the reaction of the reagent, and when the set temperature is too high, the temperature control switch 306 is used for automatically powering off at a fixed temperature, ensuring the temperature environment required by the reaction of the reagent, and ensuring the temperature of the incubation seat 302 to be constant.

In the embodiment of the present application, please refer to fig. 1, the biochemical detection module 13 is disposed on the incubation seat 302, and includes a plurality of detection seats 131 and a photoreaction assembly disposed on the detection seats 131, in this embodiment, four groups of detection seats 131, that is, four detection positions, are disposed, the four groups of detection seats 131 are uniformly distributed on the upper surface of the incubation seat 302, of course, more detection positions can be disposed according to the arrangement of structural space, the detection seats 131 are used for placing the reaction cup 16 for reagent detection, the photoreaction assembly is used for forming a photoreaction channel, the bottom of the reaction cup 16 is disposed in the center of the detection channel, and the detection is performed by the photoreaction assembly.

Any one group of photoreaction components can form a photodetection channel, each photodetection channel passes through the reaction cup 16 to detect project parameters of the same wavelength, and the photodetection channel is perpendicular to the reaction cup 16, in this embodiment, a plurality of groups of photoreaction components are arranged on any one detection seat 131, a plurality of groups of light emitting parts 132 are used for emitting light waves of different wavelengths, and the centers of the plurality of photodetection channels are collected at the bottom of the reaction cup 16 so as to meet different project tests according to different measurement wavelengths.

Referring to fig. 1, the photoreaction assembly includes a light emitting portion 132, a light receiving portion 133, a light condensing portion 134 and a light filtering portion 135, wherein the light emitting portion 132 is used for emitting a light source 1321 through the reaction cup 16, and a receiving chip 1331 disposed at the light receiving portion 133 is used for receiving a light wave passing through the reaction cup 16 and converting the light intensity into an electrical signal through a series of circuits for calculating the concentration of a measured substance. The light condensing portion 134 and the light filtering portion 135 are disposed on the light receiving portion 133 side, the light condensing portion 134 is used for condensing light waves transmitted through the reaction cup 16, and the light filtering portion 135 is used for filtering the stray light so as to accurately measure light waves with specific wavelengths.

Wherein, the light emitting part 132 is provided with a light source 1321, different emitting devices can be provided with light sources 1321 with different wavelengths so as to detect different projects, the light condensing part 134 adopts a lens, the light transmitted through the reaction cup 16 can be condensed, and the filtering part 135 adopts a filter to filter out the stray light; that is, the light source 1321 at the light emitting part 132 emits light waves, the light waves pass through the reaction cup 16 and reach the light receiving part 133, the light waves after light condensation by a lens and color filtration by a filter are converted into electric signals by the receiving chip 1331 and a series of circuits, and the concentration of the measured substance is calculated. The light source 1321, the lens, the filter, and the receiving chip 1331 of the receiving device are on the same central line.

Specifically, referring to fig. 1, the detecting seat 131 is configured as a multi-sided cylinder structure, the top wall and the bottom wall of the detecting seat 131 are horizontal, a detecting placement hole 1311 for placing the reaction cup 16 is formed in the center of the top wall of the detecting seat 131, and the side surfaces of the periphery of the detecting seat 131 are configured as a double number; the light emitting portion 132 and the light receiving portion 133 are distributed on two side surfaces of the symmetrical arrangement of the detecting seat 131, and the detecting seat 131 is correspondingly provided with a plurality of light path holes for light waves to pass through, and the plurality of light path holes are communicated with the detecting placement hole 1311.

Referring to fig. 1, the reaction cup 16 includes, from top to bottom, a large diameter section 161 and a small diameter section 162, where the diameter of the large diameter section 161 is greater than that of the detection placement hole 1311 and is used to be limited on the top wall of the detection seat 131 for positioning the reaction cup 16, and the small diameter section 162 extends into the detection placement hole 1311 and is placed in the center of intersection of multiple light path holes, so that each group of photoreaction components are all samples acting on the same reaction cup 16, and because the detection reading time is very short, so as to avoid the mutual influence of all light channels, the reaction cup can be sequentially read according to the test time of the project.

For example, the detecting seat 131 is hexahedral, and has four side walls except the top wall and the bottom wall, two sets of light reaction components can be disposed opposite to each other, and the light emitting device and the light receiving device of the same light reaction component are disposed on two opposite sides of the detecting seat 131, that is, two detecting channels are disposed. Or in another embodiment, the detecting seat 131 is an octahedron, six side walls are arranged except the top wall and the bottom wall, three groups of light reaction components can be arranged oppositely, correspondingly, three detecting channels can be formed, simultaneous detection of three different test items can be met, and more detecting channels can be formed under the condition that space allows by analogy.

The application provides a biochemical immune integrated machine integrating biochemical detection and immune analysis detection functions, which can detect the same or different items simultaneously, has large detection capacity, fully-automatic detection process, effectively improves detection efficiency, and has simple integral structure, small occupied area and high economic benefit.

The foregoing description of the invention has been presented for purposes of illustration and description, and is not intended to be limiting. Several simple deductions, modifications or substitutions may also be made by a person skilled in the art to which the invention pertains, based on the idea of the invention.

Claims (10)

1. A biochemical immunity all-in-one machine, characterized by comprising: the device comprises a rack (1), a reagent processing module (14), a scanning module (6), a sample processing module (2), an incubation module (3), a pipetting device (10), a cleaning device module (4), a mixing reading module (5), a three-dimensional arm module (8), a substrate refrigerating assembly (9), a reaction module (11), a TIP head module (15), a biochemical detection module (13) which are arranged on the rack (1), and a waste box module (7), a cleaning liquid and a waste liquid barrel (12) which are arranged on the outer side of the rack (1);

The sample processing module (2) is provided with a sample rack for arranging a plurality of sample tubes; the scanning module (6) is used for reading test strip information of a sample; the incubation module (3) is used for placing the reaction cup (16) and providing an incubation reaction environment for the reagent in the reaction cup (16); the pipetting device (10) is used for sucking, transferring and mixing reagents or samples;

The cleaning device module (4) is used for cleaning magnetic beads; the mixing reading module (5) is used for vibrating the uniform reaction cup (16), detecting the luminous value of the reaction and outputting the luminous value to a computer end for result conversion; the three-dimensional arm module (8) is used for transferring reagents, samples or reaction cups (16); the substrate refrigeration assembly (9) is used for keeping the temperature of the reagent constant; the pipetting device (10) is arranged on the three-dimensional arm module (8) and is used for sleeving the reaction cup (16); the reaction module (11) is used for testing the reaction of each reagent and sample in the reaction process; the reagent processing module (14) is used for refrigerating, storing and uniformly mixing the reagent; the TIP head module (15) is used for placing a TIP head; the biochemical detection module (13) is used for biochemical test; the waste box module (7) is used for retracting the reaction cup (16) and the TIP head after the test; the cleaning liquid and waste liquid barrel (12) is used for placing the cleaning liquid and waste liquid.

2. The biochemical immune integrated machine according to claim 1, wherein the incubation module (3) comprises a support frame (301), an incubation seat (302) arranged on the support frame (301), a cup holder (303) and a temperature control assembly, the cup holder (303) is arranged on the incubation seat (302), and a plurality of mounting positions are arranged on the cup holder (303) and used for bearing a reaction cup (16); the incubation seat (302) is arranged on the temperature control assembly for heating the incubation seat (302).

3. The biochemical immune all-in-one machine according to claim 2, wherein the temperature control assembly comprises a heating film (304), a temperature sensor (305) and a temperature control switch (306), the heating film (304) is arranged at the bottom of the incubation seat (302), the temperature control switch (306) and the temperature sensor (305) are arranged at one side of the heating film (304), the temperature sensor (305) is used for measuring and transmitting the ambient temperature of the reaction in real time, the heating film (304) is used for heating the incubation seat (302), and the temperature control switch (306) is used for fixing the temperature and cutting off the power, so that the temperature environment required by the reaction of the reagent is ensured.

4. A biochemical immune all-in-one machine according to any one of claims 2 or 3, characterized in that the biochemical detection module (13) is arranged on the incubation seat (302) and comprises a plurality of detection seats (131) and a photoreaction component arranged on the detection seats (131), wherein the detection seats (131) are used for placing the reaction cups (16) for reagent detection, the photoreaction component is used for forming a photodetection channel, the photodetection channel passes through the reaction cups (16) for detecting project parameters with the same wavelength, and the photodetection channel is arranged perpendicular to the reaction cups (16).

5. The biochemical immune integrated machine according to claim 4, wherein the light reaction component comprises a light emitting part (132) and a light receiving part (133), the light emitting part (132) is used for emitting a light source (1321) to pass through the reaction cup (16), and the light receiving part (133) is used for receiving light waves passing through the reaction cup (16) and can convert the intensity of the light waves into an electric signal so as to calculate the concentration of a measured substance.

6. The biochemical immune integrated machine according to claim 5, wherein any one of the detecting seats (131) is provided with a plurality of groups of photoreaction components, the plurality of groups of the light emitting parts (132) are used for emitting light waves with different wavelengths, and the centers of the plurality of light detecting channels are collected at the bottom of the reaction cup (16) so as to meet different project tests according to different measuring wavelengths.

7. The biochemical immune integrated machine according to claim 6, wherein the photoreaction assembly further comprises a light condensing part (134), and the light condensing part (134) is disposed at one side of the light receiving part (133) and is used for condensing light waves transmitted through the reaction cup (16).

8. The biochemical immune integrated machine according to claim 7, wherein a filter part (135) is provided at one side of the light receiving part (133), and the filter part (135) is used for filtering the stray light.

9. The biochemical immune all-in-one machine according to any one of claims 5 to 8, wherein the detection seat (131) is provided with a multi-surface cylinder structure, a detection placement hole (1311) for placing the reaction cup (16) is formed in the top wall of the detection seat (131), the side surfaces of the detection seat (131) are provided with two numbers, the light emitting parts (132) and the light receiving parts (133) are distributed on the two side surfaces symmetrically arranged on the detection seat (131), the detection seat (131) is correspondingly provided with a plurality of light path holes for light waves to pass through, and the plurality of light path holes are communicated with the detection placement hole (1311).

10. The biochemical immune integrated machine according to claim 9, wherein the reaction cup (16) comprises a large-diameter section (161) and a small-diameter section (162) from top to bottom, the diameter of the large-diameter section (161) is larger than that of the detection placement hole (1311) and is used for being limited on the top wall of the detection seat (131), and the small-diameter section (162) extends into the detection placement hole (1311) and is arranged in the intersection center of a plurality of light path holes; the light emitting portion (132), the light path hole, and the light receiving portion (133) are disposed along the same center line and perpendicular to the axis of the small-diameter section (162).