CN111991258A

CN111991258A - Skin detoxification and oxidation resistance composition and application thereof

Info

Publication number: CN111991258A
Application number: CN202011084282.XA
Authority: CN
Inventors: 骆峰; 杨敏; 杨瑞萍; 钮琳
Original assignee: Shanghai Huiwen Biotech Corp ltd
Current assignee: Shanghai Huiwen Biotech Corp ltd
Priority date: 2020-10-12
Filing date: 2020-10-12
Publication date: 2020-11-27
Anticipated expiration: 2040-10-12
Also published as: CN111991258B

Abstract

The invention discloses a skin detoxification and oxidation resistance composition and application thereof, wherein the composition is composed of the following raw materials: 1-5% of thiotaurine; 5-15% of Cladosiphon okamuranus polysaccharide; adenosine 0.5-1%; 10-20% of composite plant extract; 5% -20% of butanediol; 3-7% of PEG-6 caprylic/capric glycerides; the balance of water; weighing medicinal rhizoma Polygonati, origanum vulgaris and Codium spinulosum powder as medicinal materials at a mass ratio of 4-5:3-4:2, mixing the medicinal materials and the solvent at a mass ratio of 1:6-10 with 40% ethanol as solvent, heating and refluxing at 70-90 deg.C for 0.5-1.5 hr, and performing rotary evaporation to obtain the final product. The invention not only can help skin in the polluted environment to detoxify and avoid damage of polycyclic aromatic hydrocarbon and heavy metal to the skin, but also can further provide a lasting antioxidation effect and prevent the problems of environmental pollution and skin aging acceleration.

Description

Skin detoxification and oxidation resistance composition and application thereof

Technical Field

The invention relates to the technical field of skin care products, in particular to a skin detoxification and oxidation resistance composition and application thereof.

Background

With the rapid growth of production and economy, the environment deteriorates year by year, and air pollution is continuously aggravated. And a variety of substances harmful to skin health are present in the contaminants. Such as: heavy metals, particulate matters such as PM2.5, and polycyclic aromatic hydrocarbon substances with phototoxic effect. Ultraviolet rays, ozone, carbon dioxide and heavy metals cause oxidation reactions inside the skin, free radicals cause collagen loss and damage DNA. The phototoxic effects caused by polycyclic aromatic hydrocarbons can severely damage the skin, including accelerating the formation of free radicals, leading to oxidative stress and even accelerated skin aging. Therefore, skin care products need to help the skin resist damage from toxic substances, while having strong and durable antioxidant capacity to protect cells from oxidative stress.

In the current cosmetics sold in the market, only a few products have the anti-pollution effect, the anti-pollution effect is mainly focused on the aspects of film formation protection, heavy metal complexing, immunity improvement and the like, and products capable of completely removing the harm of polycyclic aromatic hydrocarbon and heavy metal to the skin are not seen. For example, patent CN105055261A discloses an anti-pollution skin care product and a preparation method thereof, which is added with various natural plant components such as moringa oleifera seed extract, trehalose, tremella polysaccharide and the like, so as to protect the skin, repel atmospheric dust with positive charges and complex harmful heavy metals, reduce the damage to the skin caused by atmospheric pollution, protect cells, and reduce the damage to the skin caused by dehydration and cold and heat changes.

In addition, although there are a large number of active materials having an antioxidant effect in conventional skin care products, they are generally easily oxidized themselves, and thus cannot maintain their antioxidant ability when the skin is exposed to a polluted environment for a long period of time.

Disclosure of Invention

The invention aims to overcome the problems in the prior art and provide a skin detoxification and oxidation resistance composition which not only can help skin in the polluted environment to detoxify and avoid the damage of polycyclic aromatic hydrocarbon and heavy metal to the skin, but also can further provide a lasting oxidation resistance effect and prevent the environmental pollution and accelerate the skin aging.

In order to achieve the technical purpose and achieve the technical effect, the invention is realized by the following technical scheme:

a skin detoxification and antioxidant composition, which comprises the following raw materials:

1-5% of thiotaurine;

5-15% of Cladosiphon okamuranus polysaccharide;

adenosine 0.5-1%;

10-20% of composite plant extract;

5% -20% of butanediol;

3-7% of PEG-6 caprylic/capric glycerides;

the balance being water.

The skin detoxification and antioxidant composition as described above, preferably, the composition is composed of the following raw materials:

1-5% of thiotaurine;

5-15% of Cladosiphon okamuranus polysaccharide;

adenosine 0.5-1%;

15% of compound plant extract;

5% -20% of butanediol;

3-7% of PEG-6 caprylic/capric glycerides;

the balance being water.

1-5% of thiotaurine;

5-15% of Cladosiphon okamuranus polysaccharide;

adenosine 0.5-1%;

15% of compound plant extract;

5% -20% of butanediol;

5% of PEG-6 caprylic/capric glycerides;

the balance being water.

The skin detoxification and anti-oxidation composition as described above, preferably, the preparation process of the composite plant extract is:

weighing medicinal rhizoma Polygonati, origanum vulgaris and Codium spinulosum powder as medicinal materials according to the mass ratio of 4-5:3-4:2, mixing the medicinal materials and the solvent according to the mass ratio of 1:6-10 by using 40% ethanol as a solvent, heating and refluxing at 70-90 ℃ for 0.5-1.5h, and performing rotary evaporation to obtain an extract concentrated solution, namely the composite plant extract.

weighing medicinal rhizoma Polygonati, origanum vulgaris and Codium spinosum powder as medicinal materials according to the mass ratio of 4-5:3-4:2, mixing the medicinal materials and the solvent according to the mass ratio of 1:8 by using 40% ethanol as a solvent, heating and refluxing for 0.5-1.5h at the temperature of 70-90 ℃, and performing rotary evaporation to obtain an extract concentrated solution, namely the composite plant extract.

weighing medicinal rhizoma Polygonati, origanum vulgaris and Codium spinosum powder as medicinal materials according to the mass ratio of 4-5:3-4:2, using 40% ethanol as a solvent, mixing the medicinal materials and the solvent according to the mass ratio of 1:8, heating and refluxing at the temperature of 80 ℃ for 0.5-1.5h, and performing rotary evaporation to obtain an extract concentrated solution, namely the composite plant extract.

weighing medicinal rhizoma Polygonati, origanum vulgaris and Codium spinulosum powder as medicinal materials according to the mass ratio of 4-5:3-4:2, using 40% ethanol as a solvent, mixing the medicinal materials and the solvent according to the mass ratio of 1:8, heating and refluxing for 1h at the temperature of 80 ℃, and performing rotary evaporation to obtain an extract concentrated solution, namely the composite plant extract.

Use of a skin detoxification and antioxidant composition as described above for the preparation of a skin care product.

For use as described above, the composition is preferably present in the skin care product in an amount of from 1 to 5% by weight.

For use as described above, preferably, the skin care product is a skin care product that is applied when exposed to the outside for a long period of time.

The invention has the beneficial effects that:

1. the composition provided by the invention has a remarkable anti-pollution effect, can comprehensively resist the problems of cell damage and oxidation of the skin in a polluted environment, and can remarkably improve the redness and darkness of the skin when being applied to the skin.

2. The composition provided by the invention has scientific and reasonable formula design, can be mutually synergistic, shows the optimal skin effect, and can resist the damage of various pollutants to cells. Meanwhile, compared with the thiotaurine, the composition is not only aimed at lipid peroxidation, but also has more extensive and durable antioxidant performance.

Of course, it is not necessary for any one product that embodies the invention to achieve all of the above advantages simultaneously.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic representation of MDA effect of cells stimulated with polycyclic aromatic hydrocarbons;

FIG. 2 is a schematic representation of the effect of MDA after stimulation of the cells by automobile exhaust;

FIG. 3 is a schematic representation of the effect of fibroblast activation under polycyclic aromatic hydrocarbon stimulation;

FIG. 4 is a schematic illustration of the effect of fibroblast activity under stimulation of automobile exhaust;

FIG. 5 is a line graph showing the variation of data of erythema value (hemoglobin) in the trial period of six groups of patients.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

The embodiment is a skin detoxification and anti-oxidation composition, which is composed of the following raw materials:

5% of thiotaurine;

15% of Cladosiphon okamuranus polysaccharide;

adenosine 1%;

15% of compound plant extract;

15% of butanediol;

5% of PEG-6 caprylic/capric glycerides;

the balance being water.

In this embodiment, the preparation process of the composite plant extract includes:

weighing medicinal rhizoma polygonati, origanum vulgaris and codium spinulosum powder as medicinal materials according to the mass ratio of 4:4:2, using 40% ethanol as a solvent, mixing the medicinal materials and the solvent according to the mass ratio of 1:8, heating and refluxing for 1h at the temperature of 80 ℃, and performing rotary evaporation to obtain an extract concentrated solution, namely the composite plant extract.

Example 2

1% of thiotaurine;

cladosiphon okamuranus polysaccharide 5%;

adenosine 0.5%;

15% of compound plant extract;

20% of butanediol;

5% of PEG-6 caprylic/capric glycerides;

the balance being water.

Example 3

3% of thiotaurine;

8% of Cladosiphon okamuranus polysaccharide;

adenosine 0.6%;

10% of compound plant extract;

20% of butanediol;

3% of PEG-6 caprylic/capric glycerides;

the balance being water.

weighing medicinal rhizoma polygonati, origanum vulgaris and codium spinulosum powder as medicinal materials according to the mass ratio of 5:3:2, using 40% ethanol as a solvent, mixing the medicinal materials and the solvent according to the mass ratio of 1:6, heating and refluxing for 1.5h at the temperature of 70 ℃, and performing rotary evaporation to obtain an extract concentrated solution, namely the composite plant extract.

Example 4

4% of thiotaurine;

10% of Cladosiphon okamuranus polysaccharide;

adenosine 0.9%;

20% of compound plant extract;

5% of butanediol;

7% of PEG-6 caprylic/capric glycerides;

the balance being water.

weighing medicinal rhizoma polygonati, origanum vulgaris and codium spinulosum powder as medicinal materials according to the mass ratio of 4:3:2, using 40% ethanol as a solvent, mixing the medicinal materials and the solvent according to the mass ratio of 1:10, heating and refluxing at the temperature of 90 ℃ for 0.5h, and performing rotary evaporation to obtain an extract concentrated solution, namely the composite plant extract.

In the four examples, the efficacy of example 1 is the strongest, and example 2 is the lowest-acting example. The thiotaurine is from Shanghai Huiwen Biotechnology GmbH, and its preparation method is disclosed in CN 110698376A. The taurine-containing antioxidant is a stable antioxidant, and the main mechanism is that under the stimulation of pollutants (polycyclic aromatic hydrocarbons and heavy metals), the hypotaurine is generated through decomposition reaction, and then the hypotaurine and glutathione in a body jointly generate oxidation-reduction reaction to generate the taurine. Finally, the derivative can be further converted into taurocholic acid to play an anti-inflammatory role.

The Cladosiphon okamuranus polysaccharide is from Shanghai Huiwen Biotechnology GmbH, and its preparation method is disclosed in CN 110655588A. It is a mucopolysaccharide of natural origin, which has a good biological barrier and immunity. The sulfuric acid ester functional group carried by the compound is a characteristic functional group of the compound, so that the compound has good synergistic effect with thiotaurine.

Adenosine, purchased from the plum organism, is a precursor for ATP synthesis and provides sufficient energy to the cells.

The compound extract can further supplement the antioxidant performance of the thiotaurine and provide the cell detoxification function and the immune mechanism.

Comparative example 1

This example is a composition comprising the following raw materials:

5% of thiotaurine;

15% of Cladosiphon okamuranus polysaccharide;

adenosine 1%;

15% of compound plant extract;

15% of butanediol;

5% of PEG-6 caprylic/capric glycerides;

the balance being water.

Comparative example 2

This example is a composition comprising the following raw materials:

5% of thiotaurine;

4% of Cladosiphon okamuranus polysaccharide;

adenosine 1%;

15% of compound plant extract;

15% of butanediol;

5% of PEG-6 caprylic/capric glycerides;

the balance being water.

Comparative example 3

This example is a composition comprising the following raw materials:

5% of thiotaurine;

15% of Cladosiphon okamuranus polysaccharide;

15% of compound plant extract;

15% of butanediol;

5% of PEG-6 caprylic/capric glycerides;

the balance being water.

Comparative example 4

This example is a composition comprising the following raw materials:

5% of thiotaurine;

15% of Cladosiphon okamuranus polysaccharide;

adenosine 1%;

15% of butanediol;

5% of PEG-6 caprylic/capric glycerides;

the balance being water.

The invention selects examples 1-2 and comparative examples 1-4 to carry out efficacy test:

lipid peroxidation test (in vitro experiment)

The test principle is as follows: skin cells are damaged under the stimulation of pollutants (polycyclic aromatic hydrocarbons) to cause peroxidation of membrane lipids of tissues or organs, and Malondialdehyde (MDA) is one of products of membrane lipid peroxidation and can directly reflect the damage degree of the membrane.

Test results are shown in fig. 1 and 2: examples 1 and 2 can obviously reduce the content of malondialdehyde which is a lipid peroxidation product under the stimulation of polycyclic aromatic hydrocarbon and automobile exhaust, and show that the lipid peroxidation product has a certain anti-pollution effect.

Second, lipid peroxidation test (in vitro experiment)

The test principle is as follows: in the research, primary cultured fibroblasts are used as research objects, and the influence of pollutants (polycyclic aromatic hydrocarbons and automobile exhaust) on the biological characteristics of the fibroblasts and the damage repair condition of the fibroblasts in the embodiment are observed.

The test results are shown in fig. 3 and 4: the activity of the fibroblast is obviously reduced under the stimulation of polycyclic aromatic hydrocarbon and automobile exhaust collecting fluid. While examples 1 and 2 can not only avoid the decrease in the viability of fibroblasts, but can also promote the increase in the viability of fibroblasts. Examples 1 and 2 were demonstrated to be able to completely combat the damage of the cells by contaminants and to restore cell viability.

Third, anti-pollution human body efficacy test (human body experiment)

The above examples and comparative examples were prepared into essences according to the following formulas, and 90 outdoor workers were selected to perform product trial experiments, and were divided into 6 groups of 15 persons each.

The formula of the essence is as follows:

1-2, respectively trying to add the essence with anti-pollution and anti-oxidation effects prepared in the examples 1 and 2; the 3 rd to 6 th components are respectively added into the essence with the anti-pollution and anti-oxidation effects prepared in the comparative examples 1 to 4.

The above subjects were asked to apply a proper amount of the product to the face, and to slightly massage and absorb it, after cleansing the face twice a day, once in the morning and evening. The trial data of the volunteers were counted after 7 days, 14 days, 21 days and 28 days, respectively, with 7 days as a cycle.

Testing parameters: facial wrinkles (linear light intensity), erythema value (hemoglobin);

testing an instrument: VISIA-CR.

The erythema values (hemoglobin) in the trial period for six groups of subjects are shown in the following table:

time of use	Group	1	Group 2	Group 3	Group 4	Group 5	Group 6
								Day 0	349	363	358	342	375	360
7 days	310	343	360	355	355	365
							14 days	287	322	345	357	353	348
21 days	265	296	356	362	340	342
							28 days	258	302	363	368	348	344

Data changes can be seen in figure 5.

The following table shows the wrinkles (areas) of the surface in the trial period of six groups of test subjects:

and (3) testing results: outdoor workers are exposed to pollutants for a long time, skin is easy to be stimulated to generate redness, and wrinkles are accelerated to generate for a long time. Whereas the test panel using the skin care products of example 1 and example 2 showed a reduction in visible skin redness and a reduction in wrinkle area within 28 days. The data for redness improvement and wrinkle improvement were less evident using the test panel of the comparative examples 1-4 skin care products.

The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. A skin detoxification and antioxidant composition, comprising the following components:

1-5% of thiotaurine;

5-15% of Cladosiphon okamuranus polysaccharide;

adenosine 0.5-1%;

10-20% of composite plant extract;

5% -20% of butanediol;

3-7% of PEG-6 caprylic/capric glycerides;

the balance being water.

2. The skin detoxification and antioxidant composition according to claim 1, wherein the composition is comprised of the following raw materials:

1-5% of thiotaurine;

5-15% of Cladosiphon okamuranus polysaccharide;

adenosine 0.5-1%;

15% of compound plant extract;

5% -20% of butanediol;

3-7% of PEG-6 caprylic/capric glycerides;

the balance being water.

3. The skin detoxification and antioxidant composition according to claim 2, wherein the composition is comprised of the following raw materials:

1-5% of thiotaurine;

5-15% of Cladosiphon okamuranus polysaccharide;

adenosine 0.5-1%;

15% of compound plant extract;

5% -20% of butanediol;

5% of PEG-6 caprylic/capric glycerides;

the balance being water.

4. The skin detoxification and oxidation resistance composition according to any one of claims 1 to 3, wherein the process for preparing the composite plant extract comprises:

5. The skin detoxification and anti-oxidation composition according to claim 4, wherein the process for preparing the composite plant extract comprises:

6. The skin detoxification and anti-oxidation composition according to claim 5, wherein the process for preparing the composite plant extract comprises:

7. The skin detoxification and anti-oxidation composition according to claim 6, wherein the process for preparing the composite plant extract comprises:

8. Use of a skin detoxification and antioxidant composition according to any of claims 1 to 3 for the preparation of a skin care product.

9. Use according to claim 8, wherein the composition is present in the skin care product in an amount of from 1 to 5% by weight.

10. Use according to claim 8, wherein the skin care product is a skin care product to be applied upon prolonged exposure to the outside.