CN111972426A - Compound pesticide for preventing and treating drug-resistant spodoptera frugiperda and preparation method thereof - Google Patents
Compound pesticide for preventing and treating drug-resistant spodoptera frugiperda and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/32—Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
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Abstract
The invention belongs to the technical field of preparation of compound pesticides, and discloses a compound pesticide for preventing and treating drug-resistant spodoptera frugiperda and a preparation method thereof, wherein the compound pesticide for preventing and treating drug-resistant spodoptera frugiperda comprises, by mass, 30-40 parts of an active ingredient, 20-25 parts of an attractant, 10-15 parts of jasmonic acid, 8-12 parts of beer yeast powder, 6-10 parts of composite vitamin powder and 3-5 parts of an auxiliary agent. Compared with a single medicament, the pesticide composition provided by the invention has the advantages that the indoxacarb and lufenuron are used as effective components, the pesticide effect is remarkably improved, the use amount of the pesticide is reduced, the duration is long, eggs and larvae are killed at the same time, the spodoptera frugiperda can be fully killed, and the side effect is small; the attractant is added to attract the spodoptera frugiperda, so that the insecticidal effect of the compound pesticide can be fully exerted; the plant growth regulator is added, so that the damage of the pesticide to plant bodies can be reduced, and the control effect on Spodoptera frugiperda is better.
Description
Technical Field
The invention belongs to the technical field of preparation of compound pesticides, and particularly relates to a compound pesticide for preventing and treating drug-resistant Spodoptera frugiperda and a preparation method thereof.
Background
At present, spodoptera frugiperda, also known as fall armyworm, belongs to the lepidoptera noctuidae family and is a omnivorous pest. Since the invasion of China, the method has occurred in large area in China, and great threat is caused to the safe production of corn and other crops. At present, scholars in China study the influence of cantharidin and peganum harmala total alkali on Spodoptera frugiperda cells, and simultaneously study the influence of azadirachtin, lufenuron, deltamethrin and the like on the development of Spodoptera frugiperda embryos. In the prior art, the prior prevention and treatment method adopts chlorantraniliprole, lambda-cyhalothrin, emamectin benzoate, indoxacarb and other medicaments to obtain better prevention and treatment effects. Because Spodoptera frugiperda is mainly hidden and harmed in the corn heart leaves, the Spodoptera frugiperda has better hiding capability, the conventional spraying liquid medicine has larger loss, insects are not easy to contact the liquid medicine, and better control effect is difficult to achieve; the traditional spraying method cannot control the dosage of each corn plant, the control effect is difficult to achieve when the dosage is small, and the core leaves of the tender corn plants are damaged when the dosage is large, so that the corn plants are damaged and the chemical injury is caused. Meanwhile, the existing pesticide for preventing and controlling the Spodoptera frugiperda has poor prevention and control effects due to improper use, is easy to generate drug resistance subsequently, and is easy to generate adverse effects such as environmental pollution, pesticide residue and the like after long-term large-scale use.
Through the above analysis, the problems and defects of the prior art are as follows:
(1) the conventional spraying liquid medicine has large loss, so that insects are not easy to contact the liquid medicine and can hardly achieve better control effect; the traditional spraying method cannot control the dosage of each corn plant, the control effect is difficult to achieve when the dosage is small, and the core leaves of the tender corn plants are damaged when the dosage is large, so that the corn plants are damaged and the chemical injury is caused.
(2) The existing pesticide for preventing and controlling the Spodoptera frugiperda has poor prevention and control effects due to improper use, is easy to generate drug resistance in the follow-up process, and is easy to generate adverse effects such as environmental pollution, pesticide residue and the like after long-term large-scale use.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a compound pesticide for preventing and treating drug-resistant Spodoptera frugiperda and a preparation method thereof.
The invention is realized in such a way that the preparation method of the compound pesticide for preventing and treating the drug-resistant Spodoptera frugiperda comprises the following steps:
step one, beer yeast powder treatment: and weighing beer yeast powder according to the formula, soaking the beer yeast powder in sterilized deionized water for 3-5 h, taking out, and distilling and purifying by using a distillation method for later use.
Step two, pretreatment of the composite vitamin powder: weighing compound vitamin powder according to the formula, adding distilled water to completely dissolve the compound vitamin powder, adding the dissolved compound vitamin powder into mannitol solution, and checking the pH value of the solution.
Step three, preparing the composite vitamin powder: adding water for injection to full dose, adding 0.01% of activated carbon, stirring for 30-50 min at room temperature, filtering with a titanium filter stick, and filtering with a 0.22 μm microporous filter membrane to obtain clear filtrate; purifying the effective components by distillation, sterilizing the purified essence, and storing at low temperature.
Step four, preparing jasmonic acid: diallyl adipate is used as a starting material, and is reduced by NaH and reacts with 2-pentyne chloride; performing Pd catalysis to obtain cyclopentanone containing double bonds, and performing addition reaction with dimethyl malonate; NaHCO is added3Washing and purifying to obtain methyl jasmonate, and hydrolyzing to obtain jasmonic acid.
Step five, respectively weighing the missible oil, the aqueous emulsion, the microemulsion, the suspending agent and the water dispersible granules according to the formula, dispersing the missible oil and the aqueous emulsion in water, and then adding the water dispersible granules to obtain the solvent A.
Step six, adding the microemulsion into the solvent A, and fully stirring to obtain a solvent B; and adding the suspending agent into the solvent C, adding deionized water, and performing ultrasonic dispersion at 10-40 kHz for 20-30 min to obtain the solvent.
Seventhly, adding the crude indoxacarb into acetone, wherein the mass ratio of the crude indoxacarb to the acetone is 1: 1 to 2.
Step eight, setting the temperature to be 25-30 ℃, and stirring until the solution is dissolved; adding activated carbon into the dissolved solution for decoloring, performing suction filtration on the solution, and collecting filtrate.
And step nine, cooling the collected filtrate to 2-6 ℃, precipitating solid substances, performing ultrasonic treatment on the precipitated suspension, centrifuging, filtering, washing, and freeze-drying to obtain the solid active ingredients.
And step ten, sequentially adding the attractant, the jasmonic acid, the light stabilizer and the antioxidant into the solvent prepared in the step six, and slowly stirring to obtain a first mixture.
Step eleven, heating the first mixture to 30 ℃ and keeping the temperature, adding the solid active ingredients prepared in the step nine, and uniformly stirring to obtain a second mixture.
And a twelfth step of placing the second mixture in a refrigerating chamber, and refrigerating overnight at 8-10 ℃.
And step thirteen, taking out the second mixture, adding a slow release carrier into the second mixture for adsorption, and uniformly stirring to obtain the premix of the compound pesticide for preventing and treating the drug-resistant spodoptera frugiperda.
And step fourteen, placing the premix of the compound pesticide for preventing and treating the drug-resistant Spodoptera frugiperda prepared in the step thirteen in a stirrer, and stirring for 2-3 hours at 40-60 rpm to obtain the compound pesticide for preventing and treating the drug-resistant Spodoptera frugiperda.
Further, in the second step, the vitamin complex powder comprises vitamin A, vitamin B, vitamin C and vitamin K1Vitamin E, vitamin D2Vitamin D3Any three or more of them.
Further, in the second step, the method for adjusting the pH value comprises the following steps: adjusting the pH value to 6.5-9.0 by using sodium hydroxide or hydrochloric acid solution.
Further, in the ninth step, the output frequency of the ultrasonic treatment is 1500-2000 Hz, and the time of the ultrasonic treatment is 10-20 min.
Further, in the ninth step, the centrifugation and washing method comprises: repeatedly centrifuging and washing for 8-12 times under the condition of 3000-4000 r/min, and 5-10 min each time.
The invention also aims to provide the compound pesticide for preventing and treating the drug-resistant Spodoptera frugiperda, which is prepared by the preparation method of the compound pesticide for preventing and treating the drug-resistant Spodoptera frugiperda, and the compound pesticide for preventing and treating the drug-resistant Spodoptera frugiperda comprises, by mass, 30-40 parts of an active ingredient, 20-25 parts of an attractant, 10-15 parts of jasmonic acid, 8-12 parts of beer yeast powder, 6-10 parts of compound vitamin powder and 3-5 parts of an auxiliary agent.
Further, the effective components comprise indoxacarb and lufenuron.
Further, the attractant is a composition of four substances of (Z) -7-dodecen-1-ol acetate, (Z) -9-tetradecene acetate, (Z) -11-hexadecene alcohol acetate and (Z) -9-dodecen-1-ol acetate, and the mass ratio of the substances is 9: 12: 5: 8.
further, the auxiliary agents comprise a solvent, a light stabilizer, an antioxidant and a slow release carrier.
Further, the solvent is composed of water, missible oil, aqueous emulsion, microemulsion, suspending agent and water dispersible granules; the light stabilizer is one or a mixture of more of salicylic acid esters, benzophenones, benzotriazoles, substituted acrylonitrile, triazines and hindered amine ultraviolet absorbers; the antioxidant is at least one of vitamin E, ascorbic acid, sorbic acid and methyl paraben; the slow release carrier is at least one of rubber, polyethylene slow release tube or bottle and polyvinyl chloride slow release tube.
By combining all the technical schemes, the invention has the advantages and positive effects that: the compound pesticide for preventing and treating the drug-resistant spodoptera frugiperda uses the indoxacarb and the lufenuron as effective components, and compared with a single drug, the compound pesticide provided by the invention has the advantages that the pesticide effect is remarkably improved, the use amount of the pesticide is reduced, the duration is long, eggs and larvae are killed at the same time, the spodoptera frugiperda can be fully killed, the side effect is small, and the damage to plants is small. The attractant is added to attract the spodoptera frugiperda, so that the insecticidal effect of the compound pesticide can be fully exerted; the plant growth regulator is added, so that the damage of the pesticide to the plant can be reduced; the slow-release carrier is added to improve the slow-release effect of the pesticide, so that the pesticide effect is durable, and the pesticide spraying amount can be reduced. The compound pesticide disclosed by the invention is simple in preparation method, durable in pesticide effect and better in control effect on Spodoptera frugiperda.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
FIG. 1 is a flow chart of a preparation method of the compound pesticide for preventing and treating drug-resistant Spodoptera frugiperda provided by the embodiment of the invention.
Fig. 2 is a flow chart of a preparation method of the composite vitamin powder provided by the embodiment of the invention.
Fig. 3 is a flow chart of a method for preparing jasmonic acid according to an embodiment of the present invention.
FIG. 4 is a flow chart of a method for preparing a solvent using water, emulsifiable solution, an aqueous emulsion, a microemulsion, a suspending agent, and water dispersible granules provided by an embodiment of the present invention.
Fig. 5 is a flow chart of a method for mixing indoxacarb and lufenuron according to an embodiment of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a compound pesticide for preventing and treating drug-resistant Spodoptera frugiperda and a preparation method thereof, and the invention is described in detail below with reference to the accompanying drawings.
As shown in fig. 1, the preparation method of the compound pesticide for controlling drug-resistant spodoptera frugiperda provided by the embodiment of the invention comprises the following steps:
s101, beer yeast powder treatment: and weighing beer yeast powder according to the formula, soaking the beer yeast powder in sterilized deionized water for 3-5 h, taking out, and distilling and purifying by using a distillation method for later use.
S102, respectively preparing composite vitamin powder and jasmonic acid; and the solvent is prepared by using water, missible oil, aqueous emulsion, microemulsion, suspending agent and water dispersible granules.
S103, mixing the indoxacarb and the lufenuron to obtain the solid active ingredients.
And S104, sequentially adding the attractant, the jasmonic acid, the light stabilizer and the antioxidant into the solvent obtained in the S102, and slowly stirring to obtain a first mixture.
And S105, heating the first mixture to 30 ℃, keeping the temperature, adding the solid active ingredients prepared in the S103, and uniformly stirring to obtain a second mixture.
And S106, placing the second mixture in a refrigerating chamber, and refrigerating overnight at 8-10 ℃.
S107, taking out the second mixture, adding a slow release carrier into the second mixture, and stirring to obtain the premix of the compound pesticide for preventing and treating the drug-resistant Spodoptera frugiperda.
And S108, placing the premix of the compound pesticide for preventing and treating the drug-resistant Spodoptera frugiperda prepared in the step S107 into a stirrer, and stirring for 2-3 hours at 40-60 rpm to obtain the compound pesticide for preventing and treating the drug-resistant Spodoptera frugiperda.
As shown in fig. 2, in step S102 provided in the embodiment of the present invention, the preparation method of the composite vitamin powder includes:
s201, pretreating the composite vitamin powder: weighing compound vitamin powder according to the formula, adding distilled water to completely dissolve the compound vitamin powder, adding the dissolved compound vitamin powder into mannitol solution, and checking the pH value of the solution.
S202, adding water for injection to the full amount, adding 0.01% of activated carbon, stirring for 30-50 min at room temperature, filtering with a titanium filter stick, and filtering with a 0.22-micron microporous filter membrane to obtain clear filtrate.
S203, purifying the nutrient effective components by a distillation method, sterilizing the purified essence, and storing at low temperature for later use.
In step S201 provided in the embodiment of the present invention, the vitamin complex powder includes vitamin a, vitamin B, vitamin C, and vitamin K1Vitamin E, vitamin D2Vitamin D3Any three or more of them.
In step S201 provided in the embodiment of the present invention, the method for adjusting the pH value includes: adjusting the pH value to 6.5-9.0 by using sodium hydroxide or hydrochloric acid solution.
As shown in fig. 3, in step S102 provided in the embodiment of the present invention, the preparation method of jasmonic acid is:
s301, taking diallyl adipate as a starting material, reducing by NaH, and reacting with 2-pentyne chloride.
S302, obtaining cyclopentanone containing double bonds through Pd catalysis, and carrying out addition reaction with dimethyl malonate.
S303, NaHCO3Washing and purifying to obtain methyl jasmonate, and hydrolyzing to obtain jasmonic acid.
As shown in fig. 4, in step S102 provided in the embodiment of the present invention, the method for preparing the solvent by using water, emulsifiable solution, aqueous emulsion, microemulsion, suspending agent, and water dispersible granule includes:
s401, respectively weighing missible oil, an aqueous emulsion, a microemulsion, a suspending agent and water dispersible granules according to a formula, dispersing the missible oil and the aqueous emulsion in water, and then adding the water dispersible granules to obtain a solvent A.
S402, adding the microemulsion into the solvent A, and fully stirring to obtain a solvent B.
S403, adding the suspending agent into the solvent C, adding deionized water, and performing ultrasonic dispersion at 10-40 kHz for 20-30 min to obtain the solvent.
As shown in fig. 5, in step S103, the method for mixing indoxacarb and lufenuron according to the embodiment of the present invention includes:
s501, adding the crude indoxacarb into acetone, wherein the mass ratio of the crude indoxacarb to the acetone is 1: 1 to 2.
S502, stirring at a set temperature of 25-30 ℃ until the mixture is dissolved; adding activated carbon into the dissolved solution for decoloring, performing suction filtration on the solution, and collecting filtrate.
S503, cooling the collected filtrate to 2-6 ℃, precipitating solid substances, carrying out ultrasonic treatment on the precipitated suspension, centrifuging, filtering, washing, and freeze-drying to obtain solid active ingredients.
In step S503 provided by the embodiment of the present invention, the output frequency of the ultrasonic treatment is 1500 to 2000Hz, and the time of the ultrasonic treatment is 10 to 20 min.
In step S503 provided in the embodiment of the present invention, the centrifugation and washing method includes: repeatedly centrifuging and washing for 8-12 times under the condition of 3000-4000 r/min, and 5-10 min each time.
The invention also aims to provide the compound pesticide for preventing and treating the drug-resistant Spodoptera frugiperda, which is prepared by the preparation method of the compound pesticide for preventing and treating the drug-resistant Spodoptera frugiperda, and the compound pesticide for preventing and treating the drug-resistant Spodoptera frugiperda comprises, by mass, 30-40 parts of an active ingredient, 20-25 parts of an attractant, 10-15 parts of jasmonic acid, 8-12 parts of beer yeast powder, 6-10 parts of compound vitamin powder and 3-5 parts of an auxiliary agent.
The effective components provided by the embodiment of the invention comprise indoxacarb and lufenuron.
The attractant provided by the embodiment of the invention is a composition of four substances, namely (Z) -7-dodecen-1-ol acetate, (Z) -9-tetradecene acetate, (Z) -11-hexadecene alcohol acetate and (Z) -9-dodecen-1-ol acetate, wherein the mass ratio of the substances is 9: 12: 5: 8.
the auxiliary agent provided by the embodiment of the invention comprises a solvent, a light stabilizer, an antioxidant and a slow release carrier.
The solvent provided by the embodiment of the invention is composed of water, missible oil, aqueous emulsion, microemulsion, suspending agent and water dispersible granules; the light stabilizer is one or a mixture of more of salicylic acid esters, benzophenones, benzotriazoles, substituted acrylonitrile, triazines and hindered amine ultraviolet absorbers; the antioxidant is at least one of vitamin E, ascorbic acid, sorbic acid and methyl paraben; the slow release carrier is at least one of rubber, polyethylene slow release tube or bottle and polyvinyl chloride slow release tube.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, and any modification, equivalent replacement, and improvement made by those skilled in the art within the technical scope of the present invention disclosed herein, which is within the spirit and principle of the present invention, should be covered by the present invention.
Claims (10)
1. The preparation method of the compound pesticide for preventing and treating the drug-resistant Spodoptera frugiperda is characterized by comprising the following steps of:
step one, beer yeast powder treatment: weighing beer yeast powder according to a formula, soaking the beer yeast powder in sterilized deionized water for 3-5 h, taking out, and distilling and purifying by using a distillation method for later use;
step two, pretreatment of the composite vitamin powder: weighing compound vitamin powder according to a formula, adding distilled water to completely dissolve the compound vitamin powder, adding the dissolved compound vitamin powder into a mannitol solution, and checking the pH value of the solution;
step three, preparing the composite vitamin powder: adding water for injection to full dose, adding 0.01% of activated carbon, stirring for 30-50 min at room temperature, filtering with a titanium filter stick, and filtering with a 0.22 μm microporous filter membrane to obtain clear filtrate; purifying the effective nutrient components by distillation, sterilizing the purified essence, and storing at low temperature;
step four, preparing jasmonic acid: diallyl adipate is used as a starting material, and is reduced by NaH and reacts with 2-pentyne chloride; performing Pd catalysis to obtain cyclopentanone containing double bonds, and performing addition reaction with dimethyl malonate; NaHCO is added3Washing and purifying to obtain methyl jasmonate, and hydrolyzing to obtain jasmonic acid;
step five, respectively weighing missible oil, an aqueous emulsion, a microemulsion, a suspending agent and water dispersible granules according to a formula, dispersing the missible oil and the aqueous emulsion in water, and then adding the water dispersible granules to obtain a solvent A;
step six, adding the microemulsion into the solvent A, and fully stirring to obtain a solvent B; adding a suspending agent into the solvent C, adding deionized water, and performing ultrasonic dispersion at 10-40 kHz for 20-30 min to obtain a solvent;
seventhly, adding the crude indoxacarb into acetone, wherein the mass ratio of the crude indoxacarb to the acetone is 1: 1-2;
step eight, setting the temperature to be 25-30 ℃, and stirring until the solution is dissolved; adding activated carbon into the dissolved solution for decolorization, performing suction filtration on the solution, and collecting filtrate;
cooling the collected filtrate to 2-6 ℃, precipitating solid substances, performing ultrasonic treatment on the precipitated suspension, centrifuging, filtering, washing, and freeze-drying to obtain solid active ingredients;
step ten, sequentially adding the attractant, the jasmonic acid, the light stabilizer and the antioxidant into the solvent prepared in the step six, and slowly stirring to obtain a first mixture;
step eleven, heating the first mixture to 30 ℃, keeping the temperature, adding the solid active ingredients prepared in the step nine, and uniformly stirring to obtain a second mixture;
twelfth, placing the second mixture in a refrigerating chamber, and refrigerating at 8-10 ℃ overnight;
step thirteen, taking out the second mixture, adding a slow release carrier into the second mixture for adsorption, and uniformly stirring to obtain a premix of the compound pesticide for preventing and treating the drug-resistant spodoptera frugiperda;
and step fourteen, placing the premix of the compound pesticide for preventing and treating the drug-resistant Spodoptera frugiperda prepared in the step thirteen in a stirrer, and stirring for 2-3 hours at 40-60 rpm to obtain the compound pesticide for preventing and treating the drug-resistant Spodoptera frugiperda.
2. The method for preparing a compound pesticide for controlling drug-resistant Spodoptera frugiperda as claimed in claim 1, wherein in the second step, the compound vitamin powder comprises vitamin A, vitamin B, vitamin C and vitamin K1Vitamin E, vitamin D2Vitamin D3Any three or more of them.
3. The preparation method of the compound pesticide for controlling drug-resistant Spodoptera frugiperda as claimed in claim 1, wherein in the second step, the pH value is adjusted by: adjusting the pH value to 6.5-9.0 by using sodium hydroxide or hydrochloric acid solution.
4. The preparation method of the compound pesticide for preventing and treating the drug-resistant Spodoptera frugiperda as claimed in claim 1, wherein in the ninth step, the output frequency of the ultrasonic treatment is 1500-2000 Hz, and the time of the ultrasonic treatment is 10-20 min.
5. The preparation method of the compound pesticide for controlling the drug-resistant Spodoptera frugiperda as claimed in claim 1, wherein in the ninth step, the centrifugation and washing method comprises the following steps: repeatedly centrifuging and washing for 8-12 times under the condition of 3000-4000 r/min, and 5-10 min each time.
6. The compound pesticide for controlling drug-resistant spodoptera frugiperda, which is prepared by the preparation method of the compound pesticide for controlling drug-resistant spodoptera frugiperda according to any one of claims 1 to 5, is characterized in that the compound pesticide for controlling drug-resistant spodoptera frugiperda comprises, by mass, 30 to 40 parts of an active ingredient, 20 to 25 parts of an attractant, 10 to 15 parts of jasmonic acid, 8 to 12 parts of beer yeast powder, 6 to 10 parts of compound vitamin powder and 3 to 5 parts of an auxiliary agent.
7. The compound pesticide for controlling drug-resistant Spodoptera frugiperda as claimed in claim 6, wherein the effective ingredients comprise indoxacarb and lufenuron.
8. The compound pesticide for controlling drug-resistant Spodoptera frugiperda as claimed in claim 6, wherein the attractant is a combination of four substances of (Z) -7-dodecen-1-ol acetate, (Z) -9-tetradecene acetate, (Z) -11-hexadecenol acetate and (Z) -9-dodecen-1-ol acetate, and the mass ratio of the substances is 9: 12: 5: 8.
9. the compounded pesticide for controlling drug-resistant Spodoptera frugiperda as claimed in claim 6, wherein said adjuvants comprise a solvent, a light stabilizer, an antioxidant and a slow-release carrier.
10. The compound pesticide for controlling drug-resistant Spodoptera frugiperda as claimed in claim 9, wherein the solvent comprises water, missible oil, aqueous emulsion, microemulsion, suspending agent and water dispersible granule; the light stabilizer is one or a mixture of more of salicylic acid esters, benzophenones, benzotriazoles, substituted acrylonitrile, triazines and hindered amine ultraviolet absorbers; the antioxidant is at least one of vitamin E, ascorbic acid, sorbic acid and methyl paraben; the slow release carrier is at least one of rubber, polyethylene slow release tube or bottle and polyvinyl chloride slow release tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113826644A (en) * | 2021-11-08 | 2021-12-24 | 四川省农业科学院植物保护研究所 | Entomopathogenic nematode preparation for preventing and controlling spodoptera frugiperda, preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105707107A (en) * | 2016-03-31 | 2016-06-29 | 北京颖泰嘉和生物科技股份有限公司 | Insecticide composition, preparation and applications thereof |
| CN110074107A (en) * | 2019-05-14 | 2019-08-02 | 中国农业科学院植物保护研究所 | A kind of Spodopterafrugiperda attractant composition and its application |
| CN111096319A (en) * | 2019-12-13 | 2020-05-05 | 广东新景象生物工程有限公司 | Pesticide for preventing and treating spodoptera frugiperda and preparation method thereof |
-
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105707107A (en) * | 2016-03-31 | 2016-06-29 | 北京颖泰嘉和生物科技股份有限公司 | Insecticide composition, preparation and applications thereof |
| CN110074107A (en) * | 2019-05-14 | 2019-08-02 | 中国农业科学院植物保护研究所 | A kind of Spodopterafrugiperda attractant composition and its application |
| CN111096319A (en) * | 2019-12-13 | 2020-05-05 | 广东新景象生物工程有限公司 | Pesticide for preventing and treating spodoptera frugiperda and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113826644A (en) * | 2021-11-08 | 2021-12-24 | 四川省农业科学院植物保护研究所 | Entomopathogenic nematode preparation for preventing and controlling spodoptera frugiperda, preparation method and application thereof |
| CN113826644B (en) * | 2021-11-08 | 2022-12-13 | 四川省农业科学院植物保护研究所 | Entomopathogenic nematode preparation for preventing and controlling spodoptera frugiperda, preparation method and application thereof |
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