CN111972424B - Sweet cherry branching agent and preparation method and application thereof - Google Patents
Sweet cherry branching agent and preparation method and application thereof Download PDFInfo
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- 239000006085 branching agent Substances 0.000 title abstract description 14
- 238000002360 preparation method Methods 0.000 title abstract description 10
- 241001290151 Prunus avium subsp. avium Species 0.000 title description 4
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- 238000002156 mixing Methods 0.000 claims description 31
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- 108010051842 Advanced Oxidation Protein Products Proteins 0.000 description 13
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- JLJLRLWOEMWYQK-GDUNQVSHSA-N giberellic acid Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)C1C(O)=O)CC2[C@@]2(OC3=O)C1[C@]3(C)[C@@H](O)CC2 JLJLRLWOEMWYQK-GDUNQVSHSA-N 0.000 description 3
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- DJSISFGPUUYILV-UHFFFAOYSA-N UNPD161792 Natural products O1C(C(O)=O)C(O)C(O)C(O)C1OC(C(=C1O)O)=CC2=C1C(=O)C=C(C=1C=CC(O)=CC=1)O2 DJSISFGPUUYILV-UHFFFAOYSA-N 0.000 description 1
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- NPLTVGMLNDMOQE-UHFFFAOYSA-N carthamidin Natural products C1=CC(O)=CC=C1C1OC2=CC(O)=C(O)C(O)=C2C(=O)C1 NPLTVGMLNDMOQE-UHFFFAOYSA-N 0.000 description 1
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- JXTHEWSKYLZVJC-UHFFFAOYSA-N naptalam Chemical compound OC(=O)C1=CC=CC=C1C(=O)NC1=CC=CC2=CC=CC=C12 JXTHEWSKYLZVJC-UHFFFAOYSA-N 0.000 description 1
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- DJSISFGPUUYILV-ZFORQUDYSA-N scutellarin Chemical compound O1[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1OC(C(=C1O)O)=CC2=C1C(=O)C=C(C=1C=CC(O)=CC=1)O2 DJSISFGPUUYILV-ZFORQUDYSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
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- A—HUMAN NECESSITIES
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
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- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The invention discloses a sweet cherry branching agent, a preparation method and application thereof. According to the invention, through designing different proportions of different plant hormones (cytokinin, gibberellin, brassinosteroids and the like) and adding 20 formulas of auxin inhibitors, strigolactone inhibitors, silver nitrate and the like with different proportions, the branching capability of axillary buds of the sweet cherry can be obviously improved by 5 formulas through screening, namely the branching number is obviously increased; the invention respectively applies the 5 formulas to axillary buds of the current-year branch growing in the summer growing season of the sweet cherry, and finds that the branching capability of the axillary buds growing in the summer of the sweet cherry can be obviously improved by the two types of the ramification obtained by adding the auxin transport inhibitor on the basis of the formula of the ramification, wherein the ramification is composed of cytokinin, gibberellin, brassinosteroid, a strigolactone inhibitor, an ethylene synthesis inhibitor and silver nitrate.
Description
Technical Field
The invention relates to a fruit tree branching agent, in particular to a sweet cherry branching agent, a preparation method and application thereof, and belongs to the field of sweet cherry branching agents.
Background
The sweet cherry is a current-season fresh fruit which is matured and sold on the market at the earliest time in the spring in the north, is one of the tree species with the best cultivation benefit in the northern deciduous fruit trees at present, and is entitled "first fruit in spring branch". Due to good economic benefit, sweet cherry cultivation is disputed and developed across the country in recent years, and currently, a loop Bohai gulf producing area mainly comprising the Dalian, the tobacco terrace and the Qinhuang island, a long sea producing area mainly comprising Zheng, Xian and Tianshui and a high-altitude producing area in southwest are formed. However, the sweet cherry has strong top advantages and weak branching capability, axillary buds are difficult to branch, cultivation management measures such as bud picking, bud carving and pruning are required every year, and the base of a 1-year-old branch of an untrimmed sweet cherry is difficult to sprout a lateral branch, so that the management difficulty and the production cost are greatly increased, the sweet cherry cultivation method is one of the key problems which need to be solved urgently in the development of the sweet cherry industry, and is also a bottleneck for facilitating the modern labor-saving cultivation or protected cultivation production of the sweet cherry for many years.
The ideal tree body structure is a determining factor for realizing premature and high yield of the sweet cherries and also guarantees labor-saving and efficient production in an orchard. The formation of a certain number of effective branches on the saplings is an effective way for shortening the growth period of the saplings of the sweet cherry trees and forming a preset ideal tree shape. However, no growth regulator (or a branchier) effective for increasing the number of branches of sweet cherries has been found so far at home and abroad. Therefore, there is an urgent need for a growth regulator (a brancher) which can be used not only before germination in spring but also in summer season and is effective in increasing the number of branches of sweet cherries.
Disclosure of Invention
The invention mainly aims to provide the branching agent for effectively increasing the branching number of sweet cherries, and the branching agent can be used before germination in spring and can also be used in summer growing season;
another object of the present invention is to provide a method for preparing said hair-pin.
The above object of the present invention is achieved by the following technical solutions:
a kind of branchial for increasing the branch number of sweet cherry consists of active component and solvent; wherein the active ingredients are cytokinin (6-BA), Gibberellin (GA)3) Brassinosteroids (BR), strigolactone inhibitors (TIS-108), ethylene synthesis inhibitors (AVG) and AgNO3Composition is carried out; the solvent is ethanol, acetone or water.
The concentrations of the above components are preferably: cytokinin (6-BA)60-360 mg. L-1Gibberellin (GA)3)60-360mg·L-1Brassinosteroids (BR)10-150 mg.L-10.5-10 mg.L of strigolactone inhibitor (TIS-108)-1Ethylene synthesis inhibition (AVG)0.5-30 mg.L-1，AgNO3 1-100mg·L-1。
The concentrations of the above components are more preferably: cytokinin (6-BA)90-210 mg. L-1Gibberellin (GA)3)90-210mg·L-1Brassinosteroids (BR)20-50 mg.L-11.0-3.0 mg.L of strigolactone inhibitor (TIS-108)-1Ethylene Synthesis inhibition (AVG)2.5-7.5 mg.L-1，AgNO3 5-20mg·L-1。
The concentrations of the above components are most preferably: cytokinin (6-BA)120 mg. L-1Gibberellin (GA)3)150mg·L-1Brassinosteroids (BR)30 mg.L-1Strigolactone inhibitor (TIS-108)2 mg.L-1Ethylene Synthesis inhibition (AVG)5 mg.L-1，AgNO3 15mg·L-1。
The invention further provides a method for preparing the hair-pin, which comprises the following steps:
(1) mixing cytokinin (6-BA) and Gibberellin (GA)3) Dissolving Brassinolide (BR) and AVG in absolute ethyl alcohol to obtain a solvent 1;
(2) dissolving a strigolactone inhibitor (TIS-108) in 100% acetone to obtain a solvent 2;
(3) mixing AgNO3Dissolving in water to obtain a solvent 3;
(4) dissolving the solvent 1, the solvent 2 and the solvent 3 in 25% ethanol, and mixing uniformly to obtain the final product.
Experiments show that the branching effect of the sweet cherries can be more effectively improved by adding the auxin transport inhibitor (NPA) on the basis of the composition of the active ingredients of the above-mentioned branching agent.
Thus, the invention further provides another sweet cherry branching element, which consists of an active ingredient and a solvent; wherein the active ingredients are cytokinin (6-BA), Gibberellin (GA)3) Brassinosteroids (BR), strigolactone inhibitors (TIS-108), ethylene synthesis inhibitors (AVG), auxin transport inhibitors (NPA), AgNO3Composition is carried out; the solvent is ethanol, acetone or water.
In order to achieve a better branching effect, the concentrations of the above components are preferably: cytokinin (6-BA)60-360 mg. L-1Gibberellin (GA)3)60-360mg·L-1Brassinosteroids (BR)10-150 mg.L-10.5-10 mg.L of strigolactone inhibitor (TIS-108)-1Ethylene synthesis inhibition (AVG)0.5-30 mg.L-1Auxin transport inhibitor (NPA)1-100 mg. L-1，AgNO31-100mg·L-1。
The concentrations of the above components are more preferably: cytokinin (6-BA)90-210 mg. L-1Gibberellin (GA)3)90-210mg·L-1Brassinosteroids (BR)20-50 mg.L-11.0-3.0 mg.L of strigolactone inhibitor (TIS-108)-1Ethylene Synthesis inhibition (AVG)2.5-7.5 mg.L-1Auxin transport inhibitor (NPA)5-20 mg. L-1，AgNO3 5-20mg·L-1。
The concentrations of the above components are most preferably: cytokinin (6-BA)120 mg. L-1Gibberellin (GA)3)150mg·L-1Brassinosteroids (BR)30 mg.L-1Strigolactone inhibitor (TIS-108)2 mg.L-1Ethylene Synthesis inhibition (AVG)5 mg.L-1AuxinTransport inhibitor (NPA)10 mg.L-1，AgNO3 15mg·L-1。
The invention further provides a method for preparing the hair-pin, which comprises the following steps:
(1) mixing cytokinin (6-BA) and Gibberellin (GA)3) Dissolving Brassinolide (BR), auxin transport inhibitor (NPA) and ethylene synthesis inhibitor (AVG) in absolute ethyl alcohol to obtain a solvent 1;
(2) dissolving a strigolactone inhibitor (TIS-108) in 100% acetone to obtain a solvent 2;
(3) mixing AgNO3Dissolving in water to obtain a solvent 3;
(4) dissolving the solvent 1, the solvent 2 and the solvent 3 in 25% ethanol, and mixing uniformly to obtain the final product.
For reference, the invention provides a use method for promoting the sprouting of sweet cherries by applying the sprouting element, which comprises the following steps: (1) spring festival treatment: before the fruit tree sprouts or when the sprouts are just exposed to green, cotton swabs are respectively dipped in solvent solutions with different concentrations and are smeared at the axillary buds at the middle part and below the middle part of the annual branch;
(2) and (4) summer treatment: in the growth season of axillary buds in summer (6-8 months), the solution dipped with the ramigenin is smeared at the axillary buds at the middle part and below the middle part of the current-year branch.
According to the invention, through designing different proportions of different plant hormones (cytokinin, gibberellin, brassinosteroids and the like) and adding 20 formulas of auxin inhibitors, strigolactone inhibitors, silver nitrate and the like with different proportions, 5 formulas are screened to obviously improve the branching capability of axillary buds, namely the branching number is obviously increased. Further applying the above 5 formulas to axillary buds of current-year-old branches grown in summer (6-8 months) of sweet cherry, and finding out that the axillary buds are composed of cytokinin (6-BA) and Gibberellin (GA)3) Brassinosteroids (BR), strigolactone inhibitors (TIS-108), ethylene synthesis inhibitors (AVG) and AgNO3The composed branching agent and the branching agent obtained by adding the component of auxin transport inhibitor (NPA) on the basis of the formula of the branching agent can more obviously improve the branching capability of axillary buds growing in summer of the sweet cherry, i.e. the branching number is obviously increased。
Drawings
The branch rate of 1-year branch axillary buds of the 120-species scutellarin formula before spring germination is shown.
FIG. 25 shows that the branching rate of axillary buds of the current-year branch is treated by the branching agent in summer.
Detailed Description
The invention is further described below in conjunction with specific embodiments, the advantages and features of which will become apparent from the description. These examples are illustrative only and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be within the scope of the invention.
Example 1 preparation of sweet cherry Flexin
(1) Mixing cytokinin (6-BA) and Gibberellin (GA)3) Dissolving Brassinolide (BR) and AVG in absolute ethyl alcohol to obtain a solvent 1;
(2) dissolving a strigolactone inhibitor (TIS-108) in 100% acetone to obtain a solvent 2;
(3) mixing AgNO3Dissolving in water to obtain a solvent 3;
(4) dissolving solvent 1, solvent 2 and solvent 3 in 25% ethanol, and controlling the final concentration of 6-BA at 60 mg.L-1Control of GA3Has a final concentration of 60 mg.L-1Controlling final concentration of BR to 10 mg.L-1Controlling the final concentration of AVG to be 0.5 mg.L-1Controlling the final concentration of TIS-108 to be 0.5 mg.L-1Control of AgNO3Has a final concentration of 1 mg.L-1And uniformly mixing to obtain the product.
Example 2 preparation of sweet cherry Flexin
(1) Mixing cytokinin (6-BA) and Gibberellin (GA)3) Dissolving Brassinolide (BR) and AVG in absolute ethyl alcohol to obtain a solvent 1;
(2) dissolving a strigolactone inhibitor (TIS-108) in 100% acetone to obtain a solvent 2;
(3) mixing AgNO3Dissolving in water to obtainTo solvent 3;
(4) dissolving solvent 1, solvent 2 and solvent 3 in 25% ethanol, and controlling the final concentration of 6-BA to 360 mg.L-1Control of GA3Has a final concentration of 360 mg.L-1The final concentration of BR was controlled to 150 mg.L-1Controlling the final concentration of AVG to be 30 mg.L-1Controlling the final concentration of TIS-108 to be 10 mg.L-1Control of AgNO3Has a final concentration of 100 mg.L-1And uniformly mixing to obtain the product.
Example 3 preparation of sweet cherry Flexin
(1) Mixing cytokinin (6-BA) and Gibberellin (GA)3) Dissolving Brassinolide (BR) and AVG in absolute ethyl alcohol to obtain a solvent 1;
(2) dissolving a strigolactone inhibitor (TIS-108) in 100% acetone to obtain a solvent 2;
(3) mixing AgNO3Dissolving in water to obtain a solvent 3;
(4) dissolving solvent 1, solvent 2 and solvent 3 in 25% ethanol, and controlling the final concentration of 6-BA at 120 mg.L-1Control of GA3Has a final concentration of 150 mg.L-1The final concentration of BR was controlled to 30 mg.L-1Controlling the final concentration of AVG to be 5 mg.L-1Controlling the final concentration of TIS-108 to be 2 mg.L-1Control of AgNO3Has a final concentration of 15 mg.L-1And uniformly mixing to obtain the product.
Example 4 preparation of sweet cherry Flexin
(1) Mixing cytokinin (6-BA) and Gibberellin (GA)3) Dissolving Brassinolide (BR), auxin transport inhibitor (NPA) and ethylene synthesis inhibitor (AVG) in absolute ethyl alcohol to obtain a solvent 1;
(2) dissolving strigolactone inhibitor (TIS-108) in 100% acetone to obtain solvent 2;
(3) mixing AgNO3Dissolving in water to obtain a solvent 3;
(4) dissolving solvent 1, solvent 2 and solvent 3 in 25% ethanol, and controlling the final concentration of 6-BA at 60 mg.L-1Control of GA3Has a final concentration of 60 mg.L-1Controlling final concentration of BR to 10 mg.L-1Control of the terminal of AVGThe concentration is 0.5 mg.L-1The final concentration of NPA is controlled to be 1 mg.L-1Controlling the final concentration of TIS-108 to be 0.5 mg.L-1Control of AgNO3Has a final concentration of 1 mg.L-1And uniformly mixing to obtain the product.
Example 5 preparation of sweet cherry Flexin
(1) Mixing cytokinin (6-BA) and Gibberellin (GA)3) Dissolving Brassinolide (BR), auxin transport inhibitor (NPA) and ethylene synthesis inhibitor (AVG) in absolute ethyl alcohol to obtain a solvent 1;
(2) dissolving a strigolactone inhibitor (TIS-108) in 100% acetone to obtain a solvent 2;
(3) mixing AgNO3Dissolving in water to obtain a solvent 3;
(4) dissolving solvent 1, solvent 2 and solvent 3 in 25% ethanol, and controlling the final concentration of 6-BA to 360 mg.L-1Control of GA3Has a final concentration of 360 mg.L-1The final concentration of BR was controlled to 150 mg.L-1Controlling the final concentration of AVG to be 30 mg.L-1Controlling the final concentration of TIS-108 to be 10 mg.L-1The final concentration of NPA is controlled to be 100 mg.L-1Control of AgNO3Has a final concentration of 100 mg.L-1And uniformly mixing to obtain the product.
Example 6 preparation of sweet cherry Flexin
(1) Mixing cytokinin (6-BA) and Gibberellin (GA)3) Dissolving Brassinolide (BR), auxin transport inhibitor (NPA) and ethylene synthesis inhibitor (AVG) in absolute ethyl alcohol to obtain a solvent 1;
(2) dissolving a strigolactone inhibitor (TIS-108) in 100% acetone to obtain a solvent 2;
(3) mixing AgNO3Dissolving in water to obtain a solvent 3;
(4) dissolving solvent 1, solvent 2 and solvent 3 in 25% ethanol, and controlling the final concentration of 6-BA at 120 mg.L-1Control of GA3Has a final concentration of 150 mg.L-1The final concentration of BR was controlled to 30 mg.L-1Controlling the final concentration of AVG to be 5 mg.L-1Controlling the final concentration of TIS-108 to be 2 mg.L-1The final concentration of NPA is controlled to be 10 mg.L-1Control ofAgNO production3Has a final concentration of 15 mg.L-1And uniformly mixing to obtain the product.
Test example 1 determination of the composition of the Flexin and screening test of the optimum concentration
1. Materials and methods
1.1 Experimental materials
Plant material: the European sweet cherry cultivars 'Brukas' and 'Sammi' are planted in a cherry germplasm resource garden of Zhengzhou fruit tree institute of Chinese academy of agricultural sciences, the stock is 'ZY-1', the tree age is 2 years, and the tree body grows normally.
1.2 test methods
Mixing cytokinin (6-BA) and Gibberellin (GA)3) Brassinolide (BR), auxin synthesis inhibitor (AOPP), ethylene synthesis inhibitor (AVG), Brassinolide (BR) and auxin transport inhibitor (NPA) are respectively dissolved in an absolute ethanol solvent and prepared according to concentration values shown in tables 1 to 5; dissolving strigolactone inhibitor (TIS-108) in 100% acetone solvent, and preparing according to the concentration values shown in Table 3; AgNO3The solutions were prepared in water according to the concentration values shown in Table 4.
Selecting 2 sweet cherry cultivars 'Bruce' and 'Samidori', respectively dipping cotton swabs with solvent solutions with different concentrations to be smeared at the axillary buds at the middle part and below the middle part of an annual branch before the fruit tree sprouts or when the buds just reveal green, smearing more than 40 axillary buds in each cultivar ('Bruce' and 'Samidori') by each branching element, and repeating the steps for three times biologically.
1.3 phenotypic observations and shoot count
After each component is coated for 20 days, the branch forming condition is observed, the branch forming quantity is counted, and the branch forming rate is calculated, wherein the branch forming rate is the quantity of branches/the total number of the coated axillary buds multiplied by 100.
And 1.4, data processing.
The obtained data were processed and plotted using Microsoft Excel 2010 software; shoot Rate Duncan test was performed for each variable using the one-way ANOVA method (p < 0.05).
2. Test results
The test results are shown in tables 1 to 5.
TABLE 1 selection of optimum concentrations of cytokinin (6-BA) and gibberellin (GA 3).
Table 2 selection of optimum concentration of Brassinosteroids (BR).
TABLE 3 optimal concentration selection for strigolactone inhibitor (TIS-108)
TABLE 4 auxin Synthesis inhibitor (AOPP), auxin transport inhibitor and AgNO3Selecting the optimum concentration of (3).
TABLE 5 selection of optimum concentration of ethylene Synthesis inhibitor (AVG)
Based on the test results of tables 1 to 5, the optimum final concentrations of the respective components were finally determined as follows: the optimum concentration of cytokinin (6-BA) was 120 mg.L-1Gibberellin (GA)3) The optimum concentration is 150 mg.L-1The optimum concentration of Brassinosteroids (BR) is 30 mg.L-1The optimum concentration of strigolactone inhibitor (TIS-108) is 2 mg.L-1The optimum concentration of auxin synthesis inhibitor (AOPP) is 10 mg.L-1The optimum concentration of auxin transport inhibitor (NPA) is 10 mg.L-1The optimum concentration of ethylene synthesis inhibitor (AVG) is 5 mg.L-1，AgNO3The optimum concentration is 15 mg.L-1。
Test example 2 screening test for formulation composition of sweet cherry hair restorer
1. Materials and methods
1.1 Experimental materials
Plant material: the European sweet cherry cultivars 'Brukas' and 'Sammi' are planted in a cherry germplasm resource garden of Zhengzhou fruit tree institute of Chinese academy of agricultural sciences, the stock is 'ZY-1', the tree age is 2 years, and the tree body grows normally.
Formula design of 1.220 kinds of hair-growing agent
The composition of the formula of the branchial comprises the following components: cytokinin (6-BA) and Gibberellin (GA)3) Dissolving brassinolide, AOPP and AVG in an absolute ethanol solvent; strigolactone inhibitor (TIS-108) dissolved in 100% acetone solvent; AgNO3Dissolved in water.
The chemical components of 20 formulas of the hair dye are designed, the concentration of each component in each formula adopts the optimal concentration determined by the experimental example 1, and the hair dye of 20 formulas is finally dissolved in 25% ethanol.
Formula 1: 6-BA, GA3，BR
And (2) formula: 6-BA, AOPP, GA3
And (3) formula: 6-BA, GA3，BR，TIS-108，AOPP
And (4) formula: 6-BA, GA3，BR，AOPP，AVG
And (5) formula: 6-BA, GA3，BR，AVG，AgNO3
And (6) formula: 6-BA, GA3，BR，TIS-108，AVG
And (3) formula 7: 6-BA, GA3，BR，AOPP，AVG，AgNO3
And (4) formula 8: 6-BA, TIS-108, AVG, AgNO3
Formula 9: 6-BA, GA3，TIS-108，AOPP，NPA
Formula 10: 6-BA, GA3，TIS-108，AVG，AgNO3
Formula 11: 6-BA, GA3，BR，TIS-108，AVG，AgNO3
Formula 12: 6-BA, BR, TIS-108, AVG, AgNO3
Formula 13: 6-BA, BR, TIS-108, AgNO3
Formula 14: 6-BA, GA3，BR，TIS-108，NPA，AgNO3
Formula 15: 6-BA, GA3，BR，TIS-108，AOPP，NPA
Formula 16: 6-BA, GA3，BR，TIS-108
Formula 17: 6-BA, GA3，BR，TIS-108，AVG，NPA，AgNO3
Formula 18: BR, TIS-108, AOPP, AgNO3，AVG
Formulation 19: GA3，BR，NPA，AgNO3
The formula 20 is as follows: GA3，BR，TIS-108，NPA，AOPP，AVG。
1.3 time and methods for treatment of the Flexin
Spring treatment: selecting 2 sweet cherry cultivars 'Brukes' and 'Samiedes', respectively dipping a small amount of 20 kinds of different formula of the auxin solution by using cotton swabs before or just before the fruit tree sprouts, smearing the auxin solution on the axillary buds at the middle part and below the middle part of an annual branch, smearing more than 40 axillary buds in each cultivar ('Brukes' and 'Samiedes') by each auxin, and repeating the biological process for three times.
1.4. Phenotypic observations and shoot statistics
After each kind of the branching element is smeared for 20 days, the branching condition is observed, the branching quantity is counted, and the branching rate is calculated, wherein the branching rate is the quantity of branches/the total number of smeared axillary buds multiplied by 100.
1.5. And (6) data processing.
The obtained data were processed and plotted using Microsoft Excel 2010 software; shoot Rate Duncan test was performed for each variable using the one-way ANOVA method (p < 0.05).
2. Test results
In order to detect the effect of the sweet cherry branchings with the 20 branchings formulas, the test respectively coats the 20 branchings with the 20 formula in the middle and below the middle of an annual branch before spring germination. After 30 days, it was observed and statistically analyzed.
The test results show that: compared with the control (25% ethanol solvent), 5 of the 20 formulas significantly increased the number of branches, and 5 were formula 6, formula 11, formula 14, formula 15, and formula 17, respectively. Moreover, the branching rate of the 5 formulas is more than 70 percent, and is respectively 71.89 percent, 91.36 percent, 76.53 percent, 82.52 percent and 97.35 percent, wherein the branching rate of the formula 11 and the formula 17 is the highest and reaches more than 90 percent (figure 1). However, the branching effect of the remaining 15 formulas on 1-year-old branches of the sweet cherries is not significant, the branching rate of 10 formulas is below 20% (figure 1), the branching rate of 2 formulas (formula 12 and formula 16) is between 20% and 30%, the branching rate of 2 formulas (formula 7 and formula 10) is between 30% and 40%, and the branching rate of 1 formula (formula 3) is between 40% and 50%. Further analyzing the formula components of the 5 kinds of the hair-growing agent, the results show that: BR (brassinosteroids), TIS-108 (monolignol inhibitors), AgNO3And AVG (ethylene synthesis inhibitor) can significantly improve the branching ability of sweet cherries, suggesting BR (brassinosteroids), TIS-108 (strigolactone inhibitor), AgNO3And AVG (ethylene synthesis inhibitor) plays an important role in the process of branching formation of axillary buds of sweet cherries.
In conclusion, the experiment confirms that the growth regulators of the 5 formulas (formula 6, formula 11, formula 14, formula 15 and formula 17) have remarkable promotion effects on the branching of the sweet cherries, and the formula 11 and the formula 17 have the most remarkable promotion effects on the branching of the sweet cherries.
Test example 35 kinds of branching agents comparative test on branching ability of axillary buds of sweet cherry in summer
1 materials and methods
1.1 Experimental materials
Plant material: the European sweet cherry cultivars 'Brukas' and 'Sammi' are planted in a cherry germplasm resource garden of Zhengzhou fruit tree institute of Chinese academy of agricultural sciences, the stock is 'ZY-1', the tree age is 2 years, and the tree body grows normally.
Formula of 1.2.5 kinds of hair-growing agent
The 5 formulations were formulation 6, formulation 11, formulation 14, formulation 15 and formulation 17, respectively, i.e.:
and (6) formula: 6-BA, GA3，BR，TIS-108，AVG
Formula 11: 6-BA, GA3，BR，TIS-108，AVG，AgNO3
Formula 14: 6-BA, GA3，BR，TIS-108，NPA，AgNO3
Formula 15: 6-BA, GA3，BR，TIS-108，AOPP，NPA
Formula 17: 6-BA, GA3，BR，TIS-108，AVG，NPA，AgNO3。
The concentrations of the different components of the 5 formulas mist are the optimum concentrations determined in the test example 1.
1.3 time and methods for treatment of the Flexin
And (4) summer treatment: selecting 2 sweet cherry cultivars 'Brukas' and 'Samie', respectively dipping a small amount of 5 kinds of different formula of the auxin solution by using cotton swabs in the axillary bud growth season in summer (6-8 months), and coating the solution on the axillary buds at the middle part and below the middle part of the annual branch, wherein each auxin is at least coated with more than 40 buds in each cultivar ('Brukas' and 'Samie'). The treatment is performed at least three times of biological repetition, wherein the treatment is performed once in each of 6 months, 7 months and 8 months.
1.4 phenotypic observations and shoot counts
After each kind of the branching element is smeared for 5 days, the branching condition is observed, the branching quantity is counted, and the branching rate is calculated, wherein the branching rate is the quantity of branches/the total number of smeared axillary buds multiplied by 100.
And 1.5, data processing.
The obtained data were processed and plotted using Microsoft Excel 2010 software; shoot Rate Duncan test was performed for each variable using the one-way ANOVA method (p < 0.05).
2. Test results
The current year's branches of sweet cherries treated according to formulas 6, 14 and 15 were branched by only a few axillary buds in the middle compared to the control (25% ethanol solvent). In contrast, the axillary buds of the current-year shoots treated by the formulas 11 and 17 have a significantly increased number of branches formed by the axillary buds compared with the control (25% ethanol solvent), and not only branches are formed in the middle of the current-year shoots, but also a large number of branches are formed in the middle and lower parts of the current-year shoots. After 30 days, statistics of the branching rate were carried out for the 5 formulations.
The test results show that: the axillary bud branching rate of the current-year branches of the sweet cherries treated by the formulas 6, 14 and 15 is 15-35% (fig. 2), while the axillary buds of the current-year branches treated by the formulas 11 and 17 are 65-95%, and especially the axillary bud branching rate of the current-year branches of the sweet cherries treated by the formula 17 is more than 90% (fig. 2). In addition, the axillary buds of the current-year branches are respectively treated in 6 months, 7 months and 8 months, and the observation shows that: the sprouting rate of the axillary buds of the current-year branches treated by the formulas 11 and 17 is 65-95%, and the sprouting rate has no influence on the months treated in summer (6 months, 7 months and 8 months), which suggests that the sprouting of the formulas 11 and 17 can be treated in 6-8 months.
In conclusion, the test results show that the formula 11 and the formula 17 can obviously improve the branching number of the current-year branches of the sweet cherries, and especially the formula 17 has the most obvious effect of improving the branching number of the current-year branches of the sweet cherries.
Claims (5)
1. A kind of branchial for increasing the branch number of sweet cherry consists of active component and solvent; the active ingredients are cytokinin 6-BA and gibberellin GA3Brassinosteroid BR, strigolactone inhibitor TIS-108, ethylene synthesis inhibitor AVG and AgNO3Composition is carried out; the solvent is ethanol, acetone and water;
the concentration of each component is as follows: cytokinin 6-BA 120 mg. L-1Gibberellin GA3 150 mg·L-1Brassinosteroids BR 30 mg.L-1Strigolactone inhibitor TIS-1082 mg.L-1Ethylene Synthesis inhibition AVG 5 mg.L-1，AgNO3 15 mg·L-1。
2. A method for preparing the hair restorer of claim 1, which comprises:
(1) mixing cytokinin 6-BA and gibberellin GA3Dissolving brassinolide BR and an ethylene synthesis inhibitor AVG in absolute ethyl alcohol to obtain a solvent 1;
(2) dissolving strigolactone inhibitor TIS-108 in 100% acetone to obtain solvent 2;
(3) mixing AgNO3Dissolving in water to obtain a solvent 3;
(4) dissolving the solvent 1, the solvent 2 and the solvent 3 in 25% ethanol, and mixing uniformly to obtain the final product.
3. A kind of branchial for increasing the branch number of sweet cherry consists of active component and solvent; the active ingredients are cytokinin 6-BA and gibberellin GA3Brassinosterol BR, strigolactone inhibitor TIS-108, ethylene synthesis inhibitor AVG, auxin transport inhibitor NPA and AgNO3Composition is carried out; the solvent is ethanol, acetone and water;
the concentration of each component is as follows: cytokinin 6-BA 120 mg. L-1Gibberellin GA3 150 mg·L-1Brassinosteroids BR 30 mg.L-1Strigolactone inhibitor TIS-1082 mg.L-1Ethylene Synthesis inhibition AVG 5 mg.L-1Auxin transport inhibitor NPA 10 mg.L-1，AgNO3 15 mg·L-1。
4. A method for preparing the hair restorer of claim 3, which comprises:
(1) mixing cytokinin 6-BA and gibberellin GA3The brassinosteroid BR, the auxin transport inhibitor NPA and the ethylene synthesis inhibitor AVG are dissolved in absolute ethyl alcohol to obtain a solvent 1;
(2) dissolving strigolactone inhibitor TIS-108 in 100% acetone to obtain solvent 2;
(3) mixing AgNO3Dissolving in water to obtain a solvent 3;
(4) dissolving the solvent 1, the solvent 2 and the solvent 3 in 25% ethanol, and mixing uniformly to obtain the final product.
5. Use of the beta-ramus of claim 1 or 3 for promoting the branching of sweet cherries.
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| CN106386349A (en) * | 2016-09-07 | 2017-02-15 | 山东省烟台市农业科学研究院 | Method for promoting branching of central trunk of tree-rootstock sweet cherry |
| CN109628475A (en) * | 2019-01-22 | 2019-04-16 | 中国农业科学院郑州果树研究所 | Brassinosteroid synthesizes purposes of the gene PaCYP724B1 in regulation plant branching |
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| CN109628475A (en) * | 2019-01-22 | 2019-04-16 | 中国农业科学院郑州果树研究所 | Brassinosteroid synthesizes purposes of the gene PaCYP724B1 in regulation plant branching |
Non-Patent Citations (5)
| Title |
|---|
| "AVG在果树栽培上的应用研究";于继洲;《果树科学》;19891231;第6卷(第4期);第248-251页 * |
| "Brassinosteroid-stimulated branch elongation in the marubakaido apple rootstock";A. B. Pereira-Netto et al.;《Trees》;20051216(第20期);第286-291页 * |
| "新型植物激素独脚金内酯的研究进展";陈虞超等;《中国农学通报》;20150825;第31卷(第24期);第157-162页 * |
| "桃树型及其调控关键基因研究进展";谭彬等;《果树学报》;20200410;第37卷(第4期);第599-605页 * |
| "欧洲甜樱桃中心主干形"促枝促花"技术研究进展";高华君等;《山东农业科学》;20160330;第48卷(第3期);第150-157页 * |
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