CN111957365A - Multi-range quantitative pipette and pipetting method thereof - Google Patents
Multi-range quantitative pipette and pipetting method thereof Download PDFInfo
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- CN111957365A CN111957365A CN202010853157.4A CN202010853157A CN111957365A CN 111957365 A CN111957365 A CN 111957365A CN 202010853157 A CN202010853157 A CN 202010853157A CN 111957365 A CN111957365 A CN 111957365A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses a multi-range quantitative pipette and a pipetting method thereof, wherein the multi-range quantitative pipette comprises a top air bag, a vent pipe, at least one lower air bag, a pipette and at least one horizontal connecting pipe; the top air bag and the lower air bag are provided with independent paths which do not interfere with each other and are communicated with a pipette, so that liquid is sucked in through the top air bag, each lower air bag discharges liquid to achieve the aim of quantitative liquid transfer, and redundant liquid samples are discharged through extrusion of the top air bag to achieve recovery and avoid waste; because the top air bag and the lower air bag are provided with independent paths which do not interfere with each other and are used for ventilating with the pipette, even if the top air bag is excessively extruded and filled with the whole liquid, the quantitative liquid drainage of each lower air bag is not influenced; when the lower air bags are sequentially connected with the upper air bag from top to bottom below the top air bag, the invention has the advantages of multiple ranges and more flexible and changeable use.
Description
Technical Field
The invention relates to a multi-range quantitative pipette and a pipetting method thereof.
Background
At present, a quantitative pipette is a pipetting tool for sucking a certain amount of liquid and then transferring the liquid to another place, and a conventional quantitative pipette structure is shown in fig. 1, and includes an air bag 91, a liquid storage bag 92, and a quantitative pipette 93, wherein the air bag 91 is connected to an upper end of the liquid storage bag 92, an upper end of the quantitative pipette 93 is inserted from a lower end of the liquid storage bag 92 and extends into the air bag 91 and the liquid storage bag 92, and the peripheral outer side of the quantitative pipette 93 is kept to be hermetically connected with the peripheral inner side of the lower end of the liquid storage bag 92, so that the quantitative pipette with the existing structure is formed, by squeezing the bladder 91 and then releasing, such that liquid is drawn from the dosing pipette 93 to fill the entire dosing pipette 93, excess liquid will overflow the upper end of the dosing pipette 93 into the lower reservoir 92, then the air bag 91 is squeezed again to discharge the liquid in the quantitative pipette 93, thus forming the whole sucking and pipetting process; however, the liquid overflowing the liquid storage bag 92 cannot be discharged by the squeezing air bag 91, so that a part of liquid sample is wasted, and great cost consumption is caused for expensive samples, and in addition, some novice operators have excessive force for squeezing the air bag 91, so that the quantitative pipette 93, the liquid storage bag 92 and the air bag 91 are completely filled with the liquid, and thus, the air pressure is insufficient, so that the pipetting fails; the structure of the quantitative pipette can derive various deformation structures, for example, chinese patent with publication number CN205126256U, which is to arrange a liquid storage bag in communication with one side of the upper end of the quantitative pipette and prevent backflow through an overflow pipe designed in an inclined manner, so the same drawback exists in the patent, and it is necessary to develop a pipette capable of squeezing, discharging, collecting redundant liquid samples and preventing excessive suction caused by poor control of squeezing pressure by a novice operator; in addition, the conventional quantitative pipette has only one measuring range, so that the function is relatively single, and the invention needs to develop a quantitative pipette with multiple measuring ranges.
Disclosure of Invention
Aiming at the defects of the prior art, the invention solves the problems that: the multi-range quantitative pipette and the pipetting method thereof can not only quantitatively pipette, but also squeeze, discharge and collect redundant liquid samples, prevent the defect of excessive suction caused by poor control of squeezing pressure degree by a novice operator and have multiple ranges.
In order to solve the problems, the technical scheme adopted by the invention is as follows:
a multi-range quantitative pipette comprises a top air bag, a vent pipe, at least one lower air bag, a pipette and at least one horizontal connecting pipe; the upper end of the top air bag is closed, and one side of the lower end of the top air bag is provided with a top opening; the lower part of the top air bag is sequentially connected with at least one lower air bag from top to bottom; the upper end of the lower air bag is closed, and one side of the bottom of the lower air bag is provided with a lower opening; a horizontal connecting pipe is horizontally arranged at the lower opening on one side of the bottom of the lower air bag; the pipettes are arranged on the same side of the top air bag and the lower air bag and are distributed in the vertical direction; the upper end of the vent pipe is connected with the top opening on one side of the lower end of the top air bag, and the lower end of the vent pipe is connected with the upper end of the pipette; one end of the horizontal connecting pipe is connected to the lower opening at one side of the bottom of the lower air bag, and the other end of the horizontal connecting pipe is communicated and connected to the side part of the pipette; the horizontal connecting pipe and the pipette are vertically distributed.
Further, the pipette is a glass tube or a plastic tube.
Further, two lower air bags are connected below the top air bag from top to bottom in sequence; the two lower airbags comprise a first lower airbag and a second lower airbag; the first lower air bag is positioned above the second lower air bag; the first lower air bag and the second lower air bag are respectively communicated and connected with the side part of the pipette through horizontal connecting pipes.
A pipetting method of a multi-range quantitative pipette comprises the following steps:
s1, pressing of the top airbag: firstly, pressing the top air bag, then contacting the lower end of the pipette with the liquid level to be sucked, loosening the top air bag, sucking the liquid from the lower end of the pipette and filling the whole pipette, and sucking the liquid into the vent pipe and even reaching the top air bag;
s2, quantitative pressing air bag: the first lower air bag and/or the second lower air bag are pressed to realize the quantitative liquid transfer;
s3, discharging and collecting residual liquid: the top balloon is squeezed again so that the vent tube and the remaining liquid from the pipette are expelled and collected.
Further, in step S2, the first lower bladder is pressed so that all the liquid in the pipette below the horizontal connection tube connected to the first lower bladder is discharged, thereby achieving quantitative liquid transfer in the maximum range.
Further, in step S2, the second lower air bag is pressed so that all the liquid in the pipette below the horizontal connection tube connected to the second lower air bag is discharged, thereby realizing quantitative liquid transfer.
Further, in step S2, the second lower air bag is first pressed so that all the liquid in the pipette below the horizontal connection tube connected to the second lower air bag is discharged, thereby achieving quantitative liquid transfer in one range; then the first lower air bag is pressed, so that the liquid in the pipette between the horizontal connecting pipes connected with the first lower air bag and the second lower air bag is completely discharged, and quantitative pipetting of another measuring range is realized.
The invention has the advantages of
The lower end of the top air bag and the upper end of the lower air bag are sealed, the top air bag is directly ventilated with the upper end of the pipette through the vent pipe, the lower air bag is ventilated with the side part of the pipette through the horizontal connecting pipe, so that the top air bag and the lower air bag are provided with independent and non-interfering paths to be ventilated with the pipette, liquid is sucked through the top air bag, each lower air bag discharges liquid to achieve the purpose of quantitative liquid transfer, and redundant liquid samples are discharged through extrusion of the top air bag to achieve recycling and avoid waste; because the top air bag and the lower air bag are provided with independent paths which do not interfere with each other and are used for ventilating with the pipette, even if the top air bag is excessively extruded and filled with the whole liquid, the quantitative liquid drainage of each lower air bag is not influenced; when the lower air bags are sequentially connected with the upper air bag from top to bottom below the top air bag, the invention has the advantages of multiple ranges and more flexible and changeable use.
Drawings
Fig. 1 is a schematic structural diagram of the prior art.
Fig. 2 is a schematic view showing the structure of the present invention with a lower bag.
Fig. 3 is a schematic view showing the structure of the present invention having two lower air bags.
Fig. 4 is a schematic view showing a structure of the present invention having three lower airbags.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
Example 1
As shown in fig. 2, a multi-range quantitative pipette comprises a top air bag 1, a vent pipe 3, at least one lower air bag 2, a pipette 4, at least one horizontal connecting pipe 5; the upper end of the top airbag 1 is closed, and a top opening 11 is formed in one side of the lower end of the top airbag 1; the lower part of the top airbag 1 is sequentially connected with at least one lower airbag 2 from top to bottom; the upper end of the lower air bag 2 is closed, and one side of the bottom of the lower air bag 2 is provided with a lower opening 21; a horizontal connecting pipe 5 is horizontally arranged at a lower opening 21 at one side of the bottom of the lower air bag 2; the pipettes 4 are arranged on the same side of the top air bag 1 and the lower air bag 2 and are distributed in the vertical direction; the upper end of the vent pipe 3 is connected with the top opening 11 at one side of the lower end of the top airbag 1, and the lower end of the vent pipe 3 is connected with the upper end of the pipette 4; one end of the horizontal connecting pipe 5 is connected to the lower opening 21 at one side of the bottom of the lower air bag 2, and the other end of the horizontal connecting pipe 5 is communicated and connected to the side part of the pipette 4; the horizontal connecting tube 5 and the pipette 4 are distributed vertically to each other. Further, the pipette 4 is a glass or plastic tube, the inside of the pipette 4 is a generally circular cavity structure, and the pipette capacity is determined by the inner diameter and length thereof. At least one scale mark is arranged on the pipette 4; the scale marks are horizontally aligned with the upper end surface of the inner cavity of the horizontal connecting pipe 5. And the top air bag 1, the lower air bag 2, the vent pipe 3 and one side above the pipette 4 are connected through a plastic piece 6 in a curing manner. In the present embodiment, a lower airbag 2 is optionally connected below the top airbag 1. In this way, when pipetting, the embodiment can have two modes, wherein the first mode is that the top air bag 1 is directly extruded to aspirate liquid, then the lower air bag 2 is extruded to quantitatively pipette liquid, and finally the top air bag 1 is extruded again to discharge the residual liquid for recovery; the second is directly extruding the lower air bag 2 to suck liquid, then quantitatively transferring liquid by extruding the top air bag 1, and finally extruding the lower air bag 2 again to discharge residual liquid for recovery.
Example 2
As shown in fig. 3, this embodiment is different from embodiment 1 in the number of lower air bags 2 and the pipetting method, and two lower air bags 2 are connected to the lower part of the top air bag 1 from top to bottom; the two lower airbags 2 include a first lower airbag 2a and a second lower airbag 2 b; the first lower bag 2a is positioned above the second lower bag 2 b; the first lower air cell 2a and the second lower air cell 2b are connected to the side of the pipette 4 via a horizontal connection tube 5.
A pipetting method of a multi-range quantitative pipette comprises the following steps:
s1, pressing of the top airbag: the top air bag 1 is first pressed and then the lower end of the pipette 4 is brought into contact with the liquid surface to be sucked, the top air bag 1 is released, liquid is sucked from the lower end of the pipette 4 and fills the entire pipette 4, and liquid is sucked into the vent tube 3 and even into the top air bag 1.
S2, quantitative pressing air bag: the first lower air bag 2a and/or the second lower air bag 2b are pressed to realize the purpose of quantitative liquid transfer; the step has three modes for quantitative pipetting, and can be selected according to actual requirements. Firstly, the first lower air bag 2a is pressed, so that the liquid in the pipette 4 below the horizontal connecting pipe 5 connected with the first lower air bag 2a is completely discharged, and quantitative liquid transfer with the maximum range is realized; secondly, the second lower air bag 2b is pressed, so that the liquid in the pipette 4 below the horizontal connecting pipe 5 connected with the second lower air bag 2b is completely discharged, and quantitative liquid transfer is realized; thirdly, the second lower air bag 2b is firstly pressed, so that the liquid in the pipette below the horizontal connecting pipe 5 connected with the second lower air bag 2b is completely discharged, and quantitative liquid transfer of one measuring range is realized; then, the first lower air bag 2a is pressed, so that the liquid in the pipette 4 between the horizontal connecting tubes 5 connected with the first lower air bag 2a and the second lower air bag 2b is completely discharged, and quantitative pipetting of another range is realized.
S3, discharging and collecting residual liquid: the top balloon 1 is squeezed again so that the remaining liquid in the vent tube 3 and the pipette 4 is expelled and collected.
Referring to embodiment 2, three lower airbags 2, four lower airbags 2, five lower airbags 2, and so on are connected in sequence from top to bottom below the top airbag 1, as shown in fig. 4, thus having more range.
The lower end of a top air bag 1 and the upper end of a lower air bag 2 are sealed, the top air bag 1 is directly ventilated with the upper end of a pipette 4 through a ventilating pipe 3, the lower air bag 2 is ventilated with the side part of the pipette 4 through a horizontal connecting pipe 5, so that the top air bag 1 and the lower air bag 2 are provided with independent and non-interfering paths to be ventilated with the pipette, liquid is sucked through the top air bag 1, each lower air bag 2 discharges liquid to achieve the quantitative liquid transferring purpose, and redundant liquid samples are discharged through extrusion of the top air bag 1 to achieve recycling and avoid waste; because the top air bag 1 and the lower air bag 2 are provided with independent paths which do not interfere with each other and are used for ventilating with the pipette, even if the top air bag 1 is excessively extruded and filled with the whole liquid, the quantitative liquid drainage of each lower air bag 2 is not influenced; when the lower air bags 2 are sequentially connected with the lower air bags 1 from top to bottom, the invention has the advantages of multiple ranges and more flexible and variable use.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (7)
1. A multi-range quantitative pipette is characterized by comprising a top air bag, a vent pipe, at least one lower air bag, a pipette and at least one horizontal connecting pipe; the upper end of the top air bag is closed, and one side of the lower end of the top air bag is provided with a top opening; the lower part of the top air bag is sequentially connected with at least one lower air bag from top to bottom; the upper end of the lower air bag is closed, and one side of the bottom of the lower air bag is provided with a lower opening; a horizontal connecting pipe is horizontally arranged at the lower opening on one side of the bottom of the lower air bag; the pipettes are arranged on the same side of the top air bag and the lower air bag and are distributed in the vertical direction; the upper end of the vent pipe is connected with the top opening on one side of the lower end of the top air bag, and the lower end of the vent pipe is connected with the upper end of the pipette; one end of the horizontal connecting pipe is connected to the lower opening at one side of the bottom of the lower air bag, and the other end of the horizontal connecting pipe is communicated and connected to the side part of the pipette; the horizontal connecting pipe and the pipette are vertically distributed.
2. The multi-range dosing pipette of claim 1, wherein the pipette is a glass or plastic tube.
3. The multi-range quantitative pipette according to claim 1, wherein two lower air bags are connected below the top air bag from top to bottom in sequence; the two lower airbags comprise a first lower airbag and a second lower airbag; the first lower air bag is positioned above the second lower air bag; the first lower air bag and the second lower air bag are respectively communicated and connected with the side part of the pipette through horizontal connecting pipes.
4. A pipetting method with a multirange quantitative pipette according to claim 3, characterized by the following steps:
s1, pressing of the top airbag: firstly, pressing the top air bag, then contacting the lower end of the pipette with the liquid level to be sucked, loosening the top air bag, sucking the liquid from the lower end of the pipette and filling the whole pipette, and sucking the liquid into the vent pipe and even reaching the top air bag;
s2, quantitative pressing air bag: the first lower air bag and/or the second lower air bag are pressed to realize the quantitative liquid transfer;
s3, discharging and collecting residual liquid: the top balloon is squeezed again so that the vent tube and the remaining liquid from the pipette are expelled and collected.
5. The method for pipetting a multirange quantitative pipette as claimed in claim 4, wherein in step S2, the first lower air cell is pressed so that all the liquid in the pipette below the horizontal connection tube connected to the first lower air cell is discharged, thereby realizing quantitative pipetting with a maximum range.
6. The method for pipetting a multi-range quantitative pipette according to claim 4, wherein in step S2, the second lower air bag is pressed so that the liquid in the pipette below the horizontal connection tube connected to the second lower air bag is completely discharged to perform quantitative pipetting.
7. The method for pipetting a multi-range quantitative pipette according to claim 4, wherein in step S2, the second lower air bag is first pressed so that the liquid in the pipette below the horizontal connection tube connected to the second lower air bag is completely discharged to perform quantitative pipetting in one range; then the first lower air bag is pressed, so that the liquid in the pipette between the horizontal connecting pipes connected with the first lower air bag and the second lower air bag is completely discharged, and quantitative pipetting of another measuring range is realized.
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