CN111956535A

CN111956535A - Conditioner composition and preparation method and application thereof

Info

Publication number: CN111956535A
Application number: CN201911405762.9A
Authority: CN
Inventors: 常坦然; 陈琦; 沙鸥; 连萌; 李如彦; 李庆中
Original assignee: Yunnan Hanmeng Pharmaceutical Co ltd
Current assignee: Yunnan Hanmeng Pharmaceutical Co ltd
Priority date: 2019-12-30
Filing date: 2019-12-30
Publication date: 2020-11-20

Abstract

The invention discloses a conditioner composition, a preparation method and application thereof. The composition disclosed by the invention comprises industrial hemp extract or cannabidiol, vitamin E, retinol, soybean lecithin, coenzyme Q10, vegetable oil and the like. The main components of the composition have a synergistic effect after compatibility, the bioavailability is high, the oxidation resistance is obviously improved, the composition has a good anti-aging effect, and the composition can effectively relieve skin inflammation and repair skin photodamage. Can be made into soft capsule, has stable quality, safety and no adverse side effect, can be orally taken or externally used, and can be used as conditioner for long term use.

Description

Conditioner composition and preparation method and application thereof

Technical Field

The invention belongs to the field of cosmetics, and relates to a conditioner composition, and a preparation method and application thereof.

Background

Cannabis is often used to assist in the treatment of certain advanced absolute conditions (cancer, aids), to stimulate appetite, relieve pain, to relieve neurological symptoms such as glaucoma and epilepsy, migraine, and bipolar mood swings, to relieve nausea in chemotherapy patients.

The human life generally goes through three stages of growth, maturity and aging (aging). The old age is the last stage of life and is the process of walking down a slope. During the aging stage of the human body, various organs, tissues and systems are changed. But the skin and appendages are the most likely to change in appearance. The most obvious changes of aging of people after the age of 40-50 are that hair changes from black to grey, skin changes from smooth to dull and loose, age spots appear, subcutaneous fat is reduced and denatured, skin elasticity is reduced, and skin is dry and thin. Skin aging is divided into two types, one is inherent aging, also called natural aging or endogenous aging, which is caused by genetic factors and non-resistant factors (the endocrine and immune functions of the body change along with the aging of the body); the other is exogenous aging caused by environmental factors such as ultraviolet rays, smoking, wind blowing, chemical contact and the like, wherein the long-term repeated irradiation of ultraviolet rays in sunlight is the most important factor influencing skin aging in the environment, and the factor is also called photoaging.

Endogenous aging is inevitable in the human development process, effective measures cannot be taken at present, and exogenous skin aging caused by ultraviolet rays can be prevented. The main mechanism of photoaging is that various components in the skin can induce the generation of active oxides after absorbing the energy of ultraviolet rays, directly attack cell membranes, cause lipid peroxidation, exhaust intracellular antioxidant enzymes, and accelerate the skin aging process. Meanwhile, the active oxide can also stimulate signal conduction in cells, induce skin fibroblasts to synthesize matrix metalloproteinase, and degrade various matrix components including collagen and elastin, so that the specific clinical expression and pathological change of the photoaging skin are formed.

Sunlight can cause skin damage in addition to photoaging. Wherein, the biological effect caused by the infrared ray is mainly a thermal effect, the skin can be hyperemic and form red spots after being irradiated by the infrared ray, and the skin can feel causalgia when the temperature is raised to 45 ℃. Erythema can be rapidly removed, and pigmentation can occur after repeated times; the biological effect of ultraviolet light is mainly a photochemical effect, and the photon flow with higher energy level causes the denaturation of nuclear protein and some enzymes in cells. After a latency period of 6-8 hours following irradiation, cellular changes occur and symptoms appear, including dry skin pain, shrinking epidermis, and even blistering and desquamation. Chronic, multiple exposures can cause solar keratosis (actinic keratosis) of the skin and mucous membranes, manifested by thickening of single or multiple flat-topped cornified layers, sometimes nodular, freckle-like or keratodermic, at exposed sites such as the forehead, cheek, nose tip, lips, eyelids, conjunctiva, and histopathological examination of the epithelial lining with parakeratosis, hyperkeratosis and abnormal keratosis, and loss of polarity of cell arrangement (see solar dermatitis). This is a precancerous change that may progress to skin cancer.

The main approach to reducing photoaging and photodamage is to resist oxidation: scavenging free radicals in vivo blocks the antioxidant pathway.

Most of the anti-aging products on the market at present are prepared by combining food or extracts of traditional Chinese medicines, have the function of enhancing the functions of human bodies to a certain extent, but the using effect is not ideal, and the anti-aging effect needs to be enhanced. Some of the existing anti-aging products use industrial components with strong oxidation resistance to achieve good anti-aging effect, take effect quickly in a short time, but are extremely harmful to human bodies after being used for a long time; some anti-aging products are added with vitamin antioxidants, but the products have poor stability and side effects after long-term use, for example, although vitamin E has anti-aging effect, if more than 100 mg of vitamin E is taken orally every day and is continuously applied for more than 6 months, gastrointestinal dysfunction, angular stomatitis, myasthenia, cheilitis can appear, hypoglycemia, thrombophlebitis, high blood fat and cholesterol can be caused, and the like. On the other hand, most of the existing products on the market have single function, although most of the products have certain antioxidation and anti-aging effects, for example, products containing vitamin E, vitamin C, superoxide dismutase, methionine sulfoxide reductase A, human cell protection protein, recombinant Trx-TAT-hMsrA fusion protein and other components have certain antioxidation effect, but the products can not repair damaged skin. When the skin is seriously damaged by light, urea ointment and Utrel are commonly used, but the drugs have great side effects and cannot be used for a long time. It is therefore desirable to develop a product that is effective, long-term, highly resistant to oxidation, and capable of repairing photodamaged skin.

Cannabidiol (CBD) is a non-toxic phenolic substance with high added value extracted from cannabis sativa leaves and can be used in medicines, cosmetics and health foods. Developed countries such as Israel, the United states, the United kingdom and the like have used the raw materials and developed various specific medicines and cosmetics. CBD is a non-addictive component in marijuana, can block the influence of THC on human nervous system, has pharmacological activities of antispasmodic, antirheumatic arthritis, antianxiety and the like, and also has reports on the aspects of antioxidation, anti-aging and photodamage repair, but the effect of singly using Cannabidiol (CBD) is not ideal.

Disclosure of Invention

The invention aims to provide a conditioner composition which has high oxidation resistance, can effectively repair skin photodamage, can be orally taken and externally used, and has good effects of resisting aging, lightening melanin, resisting inflammation, repairing photodamage, improving sleep and the like.

In view of the above objects, the present invention provides a composition comprising industrial cannabis extract or cannabidiol, vitamin E, retinol, soy lecithin, coenzyme Q10 and vegetable oil.

In some embodiments, in the above composition, the composition comprises, in mass percent:

the sum of the components is 100 wt%;

the Cannabidiol (CBD) may be a chemically synthesized product, a biologically synthesized product, a plant extract or prepared in other ways; preferably, the cannabidiol of the invention is a plant extract, and the plant extract part can be stem, flower, leaf, root and/or seed hulls of industrial Cannabis sativa l;

the industrial hemp extract contains 30-99% of cannabidiol by mass percentage;

preferably, the industrial hemp extract contains 50 to 99% of cannabidiol by mass percentage, more preferably 60 to 99% of cannabidiol by mass percentage, and particularly preferably 95 to 99% of cannabidiol by mass percentage.

Preferably, the industrial cannabis extract is a broad-spectrum cannabis oil, wherein the broad-spectrum cannabis oil is an industrial cannabis oil which mainly contains cannabinoids such as cannabidiol, cannabidivarin, cannabigerol and tetrahydrocannabinoid and contains active ingredients such as flavone and terpenoids, and can be directly purchased from the market;

the industrial hemp extract and cannabidiol both contain THC, do not contain any banned substances, and can be directly purchased from the market.

In some embodiments, in the above composition, the vegetable oil is one or more of hemp seed oil, sunflower seed oil, olive oil, camellia oil, wheat germ oil, and sweet almond oil, and preferably, the vegetable oil is hemp seed oil.

In some embodiments, the composition comprises the following components in percentage by mass according to utility and cost:

based on the total of the components as 100wt percent.

In some embodiments, the composition of any of the above, wherein the industrial cannabis extract or cannabidiol is present in an amount of, for example, 5 wt%, 6 wt%, 7 wt%, 8 wt%.

In some embodiments, in any of the above compositions, the vitamin E can be present in an amount of, for example, 1 wt%, 2 wt%, 3 wt%, 4 wt%.

In some embodiments, the composition of any one of the above, wherein the retinol is present in an amount of 1 wt%, 2 wt%, 3 wt%, for example.

In some embodiments, the composition of any one of the above, wherein the soy lecithin is present in an amount of, for example, 2 wt%, 3 wt%, 4 wt%.

In some embodiments, the composition of any of the above, the coenzyme Q10 can be present in an amount of, for example, 2 wt%, 3 wt%, 4 wt%.

In some embodiments, in any one of the above compositions, the composition comprises the following components in percentage by mass:

based on the total of the components as 100wt percent.

In some embodiments, in any of the above compositions, the composition further comprises one or more of peony seed oil, lemon peel oil, sea buckthorn fruit oil, and grape seed oil.

based on the total of the components as 100wt percent.

In some embodiments, in any of the above compositions, the peony seed oil may be present in an amount of, for example, 1 wt%, 2 wt%, 3 wt%, 4 wt%.

In some embodiments, the composition of any of the above, wherein the lemon peel oil is present in an amount of, for example, 1 wt%, 2 wt%, 3 wt%, 4 wt%.

In some embodiments, in any one of the above compositions, the content of the seabuckthorn fruit oil may be, for example, 1 wt%, 2 wt%, 3 wt%, 4 wt%.

In some embodiments, in any one of the above compositions, the grape seed oil may be present in an amount of, for example, 1 wt%, 2 wt%, 3 wt%, 4 wt%.

the sum of the above components is 100 wt%.

the sum of the above components is 100 wt%.

based on 100 wt% of the total mass of the composition.

based on 100 wt% of the total mass of the composition.

based on 100 wt% of the total mass of the composition.

In another aspect, the present invention also provides a method for preparing the composition as described in any one of the above, comprising the steps of adding the components other than the vegetable oil to the vegetable oil, and dissolving the components to be clear by heating and stirring at 55 to 60 ℃.

In another aspect, the present invention also provides a soft capsule consisting of a capsule shell and contents filled in the capsule shell, the contents comprising a therapeutically effective amount of the composition as described in any one of the above.

In some embodiments, in the above soft capsules, the capsule shell is a gelatin soft capsule shell.

In another aspect, the present invention also provides a process for preparing the soft capsule as described in any of the above, comprising the steps of adding the components except the vegetable oil to the vegetable oil, heating and stirring at 55-60 ℃ to dissolve until clear, and filling into the capsule shell.

In another aspect, the present invention also provides the use of any of the above compositions and/or any of the above soft capsules in the preparation of any of the following:

1) an antioxidant product;

2) products for repairing skin lighting damage;

3) products for preventing or treating skin inflammation;

4) an anti-aging product;

5) products for improving sleep;

6) products for regulating endocrine function;

7) a product for improving immunity.

The conditioner composition has the advantages that the main components in the conditioner composition have a synergistic effect after compatibility, the bioavailability is high, the oxidation resistance is obviously improved, the anti-aging effect is good, the skin inflammation can be effectively relieved, the skin photodamage can be repaired, the endocrine can be regulated, the sleep can be improved, the stability is good, the conditioner composition is safe and has no toxic or side effect, the conditioner composition can be used for a long time, the skin can be nourished and delayed by external application, the skin inflammation can be effectively relieved, the skin photodamage can be relieved, the endocrine can be regulated, the immunity of the organism can be improved by internal administration, and the free radicals in the body can be inhibited and eliminated. The composition of the present invention can be used as a conditioner for a long period of time.

Detailed Description

The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.

Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.

The present invention will be further described with reference to the following examples. It should be understood that the following examples are illustrative only and are not intended to limit the scope of the present invention.

The wide spectrum cannabis oil and cannabidiol are self-prepared samples in laboratories of Yunnan Han union pharmaceutical Co.

Example 1

Weighing 8g of broad spectrum hemp oil, 1g of vitamin E, 2g of retinol, 2g of soybean lecithin, 2g of coenzyme Q10 and 85g of hemp seed oil, sequentially adding the weighed broad spectrum hemp oil, vitamin E, retinol, soybean lecithin and coenzyme Q10 into the hemp seed oil, heating at 55-60 ℃ for 3h, stirring and dissolving until the mixture is clear, and filling the mixture into gelatin soft capsule shells, wherein each capsule is 0.58 g.

Example 2

Weighing 5g of broad-spectrum hemp oil, 4g of vitamin E, 0.1g of retinol, 0.1g of soybean lecithin, 4g of coenzyme Q10, 3g of peony seed oil and 83.8g of hemp seed oil, sequentially adding the weighed broad-spectrum hemp oil, vitamin E, retinol, soybean lecithin, coenzyme Q10 and peony seed oil into the hemp seed oil, heating for 3 hours at 55-60 ℃, stirring and dissolving to be clear, and filling into a gelatin soft capsule shell, wherein each capsule is 0.58 g.

Example 3

Weighing 10g of cannabidiol, 0.1g of vitamin E, 5g of retinol, 5g of soybean lecithin, 0.1g of coenzyme Q10, 4.8g of lemon peel oil and 75g of hemp seed oil, sequentially adding the weighed cannabidiol, vitamin E, retinol, soybean lecithin, coenzyme Q10 and lemon peel oil into the hemp seed oil, heating at 55-60 ℃ for 3h, stirring and dissolving until the mixture is clear, and filling the mixture into a gelatin soft capsule shell, wherein each capsule is 0.58 g.

Example 4

Weighing 10g of broad spectrum hemp oil, 5g of vitamin E, 5g of retinol, 5g of soybean lecithin, 5g of coenzyme Q10, 5g of lemon peel oil and 65g of hemp seed oil, sequentially adding the weighed broad spectrum hemp oil, vitamin E, retinol, soybean lecithin, coenzyme Q10 and lemon peel oil into the hemp seed oil, heating at 55-60 ℃ for 3h, stirring and dissolving until the mixture is clear, and filling the mixture into a gelatin soft capsule shell, wherein each capsule is 0.58 g.

Example 5

Weighing 5g of cannabidiol, 1g of vitamin E, 1g of retinol, 1g of soybean lecithin, 1g of coenzyme Q10, 1g of peony seed oil and 90g of hemp seed oil, sequentially adding the weighed cannabidiol, vitamin E, retinol, soybean lecithin, coenzyme Q10 and peony seed oil into the hemp seed oil, heating at 55-60 ℃ for 3h, stirring and dissolving until the mixture is clear, and filling the mixture into a gelatin soft capsule shell, wherein each capsule is 0.58 g.

Example 6

Weighing 8g of cannabidiol, 1g of vitamin E, 2g of retinol, 4g of soybean lecithin, 0.1g of coenzyme Q10, 3g of seabuckthorn fruit oil and 81.9g of hemp seed oil, sequentially adding the weighed cannabidiol, vitamin E, retinol, soybean lecithin, coenzyme Q10 and seabuckthorn fruit oil into the hemp seed oil, heating for 3 hours at 55-60 ℃, stirring and dissolving until the mixture is clear, and filling the mixture into a gelatin soft capsule shell, wherein each capsule is 0.58 g.

Comparative example 1

Weighing 7g of vitamin E, 7g of retinol, 7g of soybean lecithin, 7g of coenzyme Q10, 7g of lemon peel oil and 65g of hemp seed oil, sequentially adding the weighed vitamin E, retinol, soybean lecithin, coenzyme Q10 and lemon peel oil into the hemp seed oil, heating at 55-60 ℃ for 3h, stirring and dissolving until the mixture is clear, and filling the mixture into a gelatin soft capsule shell with 0.58g per capsule.

Comparative example 2

Weighing 11g of broad spectrum hemp oil, 6g of retinol, 6g of soybean lecithin, 6g of coenzyme Q10, 6g of lemon fruit peel oil and 65g of hemp seed oil, sequentially adding the weighed broad spectrum hemp oil, retinol, soybean lecithin, coenzyme Q10 and lemon fruit peel oil into the hemp seed oil, heating at 55-60 ℃ for 3h, stirring and dissolving until the mixture is clear, and filling the mixture into a gelatin soft capsule shell with 0.58g per capsule.

Comparative example 3

Weighing 15g of broad-spectrum hemp oil, adding into 65g of hemp seed oil, heating at 55-60 deg.C for 3h, stirring to dissolve until clear, and filling into gelatin soft capsule shell of 0.58 g/capsule.

Comparative example 4

Weighing 35g of vitamin E, adding into 65g of hemp seed oil, heating at 55-60 ℃ for 3h, stirring to dissolve until the mixture is clear, and filling into gelatin soft capsule shells of 0.58 g/capsule.

Comparative example 5

Weighing 12g of broad-spectrum hemp oil and 15g of vitamin E, adding into 65g of hemp seed oil, heating at 55-60 ℃ for 3h, stirring to dissolve until the mixture is clear, and filling into gelatin soft capsule shells at 0.58 g/capsule.

Test example 1 evaluation of antioxidant Properties

Preparing the contents of the soft capsules prepared in examples 1-6 and comparative examples 1-5 into ethanol solutions with different concentrations (each sample is prepared into the concentration of 6, 10, 15, 20, 25 and 50 mu g/mL), respectively adding 2.0mL of the prepared sample to be tested into a test tube with a plug, adding 2.0mL of 0.2mmol/L DPPH solution, shaking uniformly, reacting in a dark place for 30min, and measuring the absorbance A1 at 517nm by using absolute ethyl alcohol as a reference; and simultaneously measuring the absorbance A0 of a mixed solution of 2.0mL of 0.2mmol/L DPPH solution and 2.0mL of absolute ethyl alcohol and the absorbance A2 of a mixed solution of 2.0mL of a sample to be measured and 2.0mL of absolute ethyl alcohol. The measurement results are expressed as clearance (E) and are calculated by the formula: e ═ 1- (a 1-a 2) ]/a0 × 100%. And drawing a curve for eliminating DPPH & of the sample to be detected by taking the concentration of the sample to be detected as an abscissa and the elimination rate value as an ordinate, and calculating the concentration of the sample to be detected when the DPPH & elimination rate is 50% according to a linear regression equation of the curve, wherein the concentration is defined as a half inhibition concentration (IC 50). The DPPH-removing ability of the samples was measured by the IC50 value of the sample concentration with a removing rate of 50%, and the results are shown in Table 1.

TABLE 1 comparison of DPPH scavenging ability of samples

As can be seen from Table 1, the compositions of examples 1-6 all have very good radical scavenging effect, while the compositions of comparative examples 1-5 have a certain radical scavenging effect, but the effect is significantly less than that of the compositions of examples 1-6. Particularly, in comparative examples 3 and 4, the radical scavenging action is remarkably reduced compared with other examples and comparative examples, which shows that the antioxidant activity of the cannabidiol after being combined with vitamin E is enhanced, particularly the antioxidant activity of the cannabidiol after being combined with other components in the composition is stronger, and the synergistic effect of the components of the composition after being combined is shown.

Test example 2 animal experiment for repairing photodamage

Removing hair from back of mouse (ICR mouse, purchased from animal experiment center of Zhejiang university of traditional Chinese medicine, body weight 20 + -5 g), exposing skin of back sufficiently, and performing UVB (300 mJ/cm)²) Irradiating the mice once a day for 3 days continuously, finishing molding, and enabling the back skin of the successfully molded mice to have obvious erythema and scale change.

The successfully modeled mice were divided into 10 groups of 10 mice each, and each group of mice was administered with the contents of the soft capsules prepared in examples 1 to 6 and comparative examples 1 to 5, and the contents of the soft capsules were applied to the injured skin of the back of the mice by 1ml each day in the morning and evening, and administered for two weeks.

Skin damage status was scored on UVB-irradiated areas of the backs of the mice (see table 2 for details), and the results are shown in table 3.

TABLE 2 evaluation criteria for skin damage status of UVB-irradiated area of mouse back

TABLE 3 mice dorsal skin injury status score

As can be seen from the results in table 3, the compositions of examples 1 to 6 of the present invention have better photo-repairing effects than the composition of the comparative example, indicating that the photo-damage repairing effect is enhanced after the cannabidiol or the cannabis oil of the broad spectrum is combined with other components, and the synergistic effect is obtained.

Claims

1. A composition comprising industrial cannabis extract or cannabidiol, vitamin E, retinol, soy lecithin, coenzyme Q10, and vegetable oil.

2. The composition of claim 1, wherein: the composition comprises the following components in percentage by mass:

the balance of the vegetable oil is the vegetable oil,

based on the total of the components as 100wt percent.

3. The composition of claim 1, wherein: the composition comprises the following components in percentage by mass:

the balance of the vegetable oil is the vegetable oil,

based on the total of the components as 100wt percent.

4. The composition according to any one of claims 1 to 3, characterized in that: the vegetable oil is one or more of hemp seed oil, sunflower seed oil, olive oil, camellia oil, wheat germ oil and sweet almond oil;

preferably, the vegetable oil is hemp seed oil.

5. The composition according to any one of claims 1 to 4, characterized in that: the industrial hemp extract contains 30-99% of cannabidiol by mass percentage;

preferably, the industrial hemp extract contains 50-99% of cannabidiol by mass percentage;

preferably, the industrial cannabis extract is a broad spectrum of cannabis oils.

6. The composition of claim 1, wherein: the composition further comprises one or more of peony seed oil, lemon peel oil, sea buckthorn fruit oil, and grape seed oil.

7. The composition of claim 6, wherein: the composition comprises the following components in percentage by mass:

the balance of the vegetable oil is the vegetable oil,

based on the total of the components as 100wt percent.

8. A soft capsule comprised of a capsule shell and contents filled in the capsule shell, the contents comprising a therapeutically effective amount of the composition of any one of claims 1-7.

9. The soft capsule according to claim 8, characterized in that: the capsule shell is a gelatin soft capsule shell.

10. Use of a composition according to any one of claims 1 to 7 and/or a soft capsule according to claim 8 or 9 for the preparation of a product according to any one of the following:

1) an antioxidant product;

2) products for repairing skin lighting damage;

3) products for preventing or treating skin inflammation;

4) an anti-aging product;

5) products for improving sleep;

6) products for regulating endocrine function;

7) a product for improving immunity.