CN111937736A - Device and method for measuring plant nutrient absorption and utilization rate - Google Patents

Device and method for measuring plant nutrient absorption and utilization rate Download PDF

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Publication number
CN111937736A
CN111937736A CN202010843060.5A CN202010843060A CN111937736A CN 111937736 A CN111937736 A CN 111937736A CN 202010843060 A CN202010843060 A CN 202010843060A CN 111937736 A CN111937736 A CN 111937736A
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nutrient solution
plant
flowerpot
absorption
utilization rate
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孙海
金桥
张亚玉
张淋淋
邵财
王秋霞
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Institute Special Animal and Plant Sciences CAAS
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Institute Special Animal and Plant Sciences CAAS
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Priority to CN202010843060.5A priority Critical patent/CN111937736A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • A01G31/02Special apparatus therefor
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/0098Plants or trees
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

Abstract

The invention provides a device and a method for measuring the absorption and utilization rate of plant nutrients; the device includes: a carrier; a plurality of flowerpots which are horizontally placed on the bearing frame; non-woven fabrics are laid in the flowerpot and filled with fine sand, and a drain hole with a valve is arranged at the position, close to the bottom, of the side wall; a bucket is hoisted on the drain hole; the hanging bottles are hung on one side of the bearing frame; nutrient solution is filled in the infusion bottle and is communicated with each flowerpot through an infusion pipeline; the infusion pipeline is provided with a flow rate control valve. Compared with the prior art, the method realizes the determination of the absorption and utilization rate of the plant nutrients based on the sand culture treatment for the first time, and provides the specific sand culture device which can meet the requirement of regular detection of the plant; the device and the method provided by the invention can avoid the occurrence of root rot, and the determination result is accurate, so that the nutrient absorption rule of the plant can be effectively mastered, and a foundation is laid for scientific fertilization and management of farmland planting.

Description

Device and method for measuring plant nutrient absorption and utilization rate
Technical Field
The invention relates to the technical field of plant nutrient absorption detection, in particular to a device and a method for determining the absorption utilization rate of plant nutrients.
Background
The soil fertilizer belongs to a relatively important component in the agricultural production process, and the scientific use of the soil fertilizer in the agricultural production process can improve the condition of soil, adjust the fertility of the soil, improve the yield and quality of agricultural planting and improve the economic benefit. On the contrary, unreasonable use of soil fertilizer can cause waste of soil fertilizer and damage of ecological environment, which seriously jeopardizes agricultural production progress. Therefore, effective and scientific application and popularization of the soil fertilizer are particularly important.
Researches show that the more accurately the nutrient absorption and utilization rate of plants is mastered, the more beneficial the fertilizer application amount is reduced, the cost is saved and the environment is protected. At present, a water culture device is mostly adopted for plant nutrient absorption and utilization rate experiments and plant nutrient deficiency experiments; however, one of the biggest disadvantages of the hydroponic experiment is that the plants are easy to rot roots, so that the nutrient solution needs to be replaced frequently, which not only takes time, but also may bring large experimental errors due to the replacement of the nutrient solution for many times.
Disclosure of Invention
In view of the above, the present invention aims to provide a device and a method for determining plant nutrient absorption and utilization rate, which can avoid root rot and achieve accurate determination results, so that the plant nutrient absorption rule can be effectively mastered, and a foundation is laid for scientific fertilization and management of farmland planting.
The invention provides a device for measuring the absorption and utilization rate of plant nutrients, which comprises:
a carrier;
a plurality of flowerpots which are horizontally placed on the bearing frame; non-woven fabrics are laid in the flowerpot and filled with fine sand, and a drain hole with a valve is arranged at the position, close to the bottom, of the side wall; a bucket is hoisted on the drain hole;
the hanging bottles are hung on one side of the bearing frame; nutrient solution is filled in the infusion bottle and is communicated with each flowerpot through an infusion pipeline; the infusion pipeline is provided with a flow rate control valve.
Preferably, the flowerpot is made of PVC, the volume of the flowerpot is 5L-15L, and the amount of the filled fine sand is 1/2-4/5 of the height of the flowerpot.
Preferably, the fine sand is soaked in 0.5-1.5 mol/L hydrochloric acid for 2 days-4 days and then washed by water, and the particle size is 0.5-1 mm.
Preferably, the aperture of the drain hole is 1 cm-2 cm; the bucket is made of PVC and has a volume of 4-6L.
Preferably, the nutrient solution is selected from a complete nutrient solution or an incomplete nutrient solution.
Preferably, the complete nutrient solution has the following formula:
1.25g/L Ca(NO3)2、0.12g/L FeSO4、37g/L MgSO4、28g/L(NH4)2HPO4、41g/L KNO3、0.6g/L H3BO3、0.4g/L MnSO4、0.004g/L CuSO4、0.004g/L ZnSO4、pH5.5~6.5。
the invention also provides a method for measuring the absorption and utilization rate of plant nutrients, and the device adopting the technical scheme comprises the following steps:
a) placing the plant to be screened in fine sand of a flowerpot for cultivation and planting; adjusting environmental parameters according to the growth conditions of the plants to be screened, and then carrying out drip irrigation by adopting the nutrient solution in the hanging bottle to culture the plants;
b) detecting the concentration of each element in the initial nutrient solution, opening a valve after the plants are cultured for a certain time, collecting the residual nutrient solution in the flowerpot into a water bucket through a drain hole, detecting the concentration of each element in the residual nutrient solution, and calculating to obtain the absorption utilization rate of the plant nutrients.
Preferably, the environmental parameters in step a) include temperature, humidity, illumination type and illumination intensity.
Preferably, the flow rate of the drip irrigation in the step a) is 100mL/h to 300 mL/h.
Preferably, during the plant cultivation in the step a), the nutrient solution is dripped into the pot in an amount exceeding 1/4 of the height of the pot.
The invention provides a device and a method for measuring the absorption and utilization rate of plant nutrients; the device includes: a carrier; a plurality of flowerpots which are horizontally placed on the bearing frame; non-woven fabrics are laid in the flowerpot and filled with fine sand, and a drain hole with a valve is arranged at the position, close to the bottom, of the side wall; a bucket is hoisted on the drain hole; the hanging bottles are hung on one side of the bearing frame; nutrient solution is filled in the infusion bottle and is communicated with each flowerpot through an infusion pipeline; the infusion pipeline is provided with a flow rate control valve. Compared with the prior art, the method realizes the determination of the absorption and utilization rate of the plant nutrients based on the sand culture treatment for the first time, and provides the specific sand culture device which can meet the requirement of regular detection of the plant; the device and the method provided by the invention can avoid the occurrence of root rot, and the determination result is accurate, so that the nutrient absorption rule of the plant can be effectively mastered, and a foundation is laid for scientific fertilization and management of farmland planting.
In addition, the method for measuring the plant nutrient absorption and utilization rate is simple and rapid, and can also detect the plant leaf color change, the nutrient content in the plant body and the residual nutrient content of the nutrient solution, so that the plant nutrient absorption rule is mastered, the plant nutrient deficiency symptom is known, and the method has good popularization and application values.
Drawings
FIG. 1 is a schematic structural diagram of an apparatus for determining plant nutrient absorption and utilization rate provided by an embodiment of the invention.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a device for measuring the absorption and utilization rate of plant nutrients, which comprises:
a carrier;
a plurality of flowerpots which are horizontally placed on the bearing frame; non-woven fabrics are laid in the flowerpot and filled with fine sand, and a drain hole with a valve is arranged at the position, close to the bottom, of the side wall; a bucket is hoisted on the drain hole;
the hanging bottles are hung on one side of the bearing frame; nutrient solution is filled in the infusion bottle and is communicated with each flowerpot through an infusion pipeline; the infusion pipeline is provided with a flow rate control valve.
Referring to fig. 1, fig. 1 is a schematic structural diagram of an apparatus for determining plant nutrient absorption and utilization rate according to an embodiment of the present invention; wherein, 1 is a bearing frame, 2 is fine sand, 3 is a flowerpot, 4 is a bucket, 5 is a valve, 6 is a transfusion bottle, 7 is nutrient solution, and 8 is a flow rate control valve.
In the invention, the bearing frame mainly plays a role in supporting and fixing, so that the whole device is stable and firm.
In the invention, the plurality of flowerpots are flatly placed on the bearing frame; set up a plurality of flowerpots and can satisfy parallel test requirement, preferably 3. In the invention, the material of the flowerpot is preferably PVC; the flowerpot is made of PVC materials, the PVC materials are safe and non-toxic, harmful substances and heavy metals are not easy to leach out under the slightly acid pH condition of the nutrient solution, so that the accuracy of the research result is influenced, and meanwhile, the dark condition of the root of the crop in the soil can be further shaded and simulated, so that the accuracy and the scientificity of the research result are guaranteed. In the present invention, the volume of the flowerpot is preferably 5L to 15L, more preferably 10L; thereby satisfying the culture of various plants.
In the invention, non-woven fabrics are laid in the flowerpot and filled with fine sand, and a drain hole with a valve is arranged at the position of the side wall close to the bottom. In the invention, the non-woven fabric is air-permeable and water-permeable, and can prevent fine sand from leaking from the water drainage hole of the flowerpot.
In the invention, the fine sand is preferably soaked in 0.5-1.5 mol/L hydrochloric acid for 2 days-4 days and then washed by water; by adopting the color elution process and soaking with hydrochloric acid, the invention can remove nutrient residues and bacteria in sand and effectively reduce test errors. In the present invention, the fine sand preferably has a particle size of 0.5mm to 1 mm.
In the invention, the amount of fine sand filled in the flowerpot is preferably 1/2-4/5 of the height of the flowerpot, and more preferably 2/3 of the height of the flowerpot. The invention adopts the fine sand as the plant culture medium and is matched with nutrient solution with specific components and flow, thereby greatly improving the efficiency of the nutrient solution and avoiding the frequent replacement of the nutrient solution, thereby improving the accuracy of measuring the nutrient absorption rate of the plant.
In the invention, the drain hole is arranged at the position of the side wall close to the bottom, so that the residual nutrient solution in the flowerpot can be completely drained; the aperture of the drain hole is preferably 1 cm-2 cm.
In the invention, the drain holes are hoisted with buckets, namely, each drain hole is matched with one bucket and used for collecting residual nutrient solution drained from the drain hole. In the invention, the material of the bucket is preferably PVC; the volume of the water tank is preferably 4L to 6L, and more preferably 5L.
In the invention, the plurality of hanging bottles are hung on one side of the bearing frame; the infusion bottle can be prevented from shielding the light required by the plant to influence the test result. In the invention, nutrient solution is filled in the infusion bottle and is communicated with each flowerpot through an infusion pipeline; namely, each hanging bottle can be provided with a plurality of infusion pipelines which are respectively communicated with each flowerpot in the plurality of flowerpots, so that nutrient solution can be indiscriminately delivered to each flowerpot. In the invention, the infusion pipeline is provided with a flow rate control valve; the device is used for controlling the flow rate of the nutrient solution in each infusion pipeline, so that the nutrient solution is slowly dropped into and uniformly distributed in the sand layer of the flowerpot, and the growth condition of the plants is continuously observed.
In the present invention, the nutrient solution is preferably selected from a complete nutrient solution or an incomplete nutrient solution; wherein, the formula of the complete nutrient solution is preferably as follows:
1.25g/L Ca(NO3)2、0.12g/L FeSO4、37g/L MgSO4、28g/L(NH4)2HPO4、41g/L KNO3、0.6g/L H3BO3、0.4g/L MnSO4、0.004g/L CuSO4、0.004g/L ZnSO4pH5.5-6.5; the incomplete nutrient solution is prepared by reducing one or more components in the formula of the complete nutrient solution, and ensuring at least one component. In a preferred embodiment of the present invention, the incomplete nutrient solution has a formula of 1.25g/L Ca (NO)3)2、0.12g/L FeSO4、37g/L MgSO4、41g/L KNO3、0.6g/L H3BO3、0.4g/L MnSO4、0.004g/L CuSO4、0.004g/L ZnSO4pH5.5-6.5; the corresponding also includes 28g/L (NH)4)2HPO4The nutrient solution was used for phosphorus deficiency experiments.
In the invention, the complete nutrient solution or the incomplete nutrient solution is filled into a hanging bottle for experiment according to the needs in the growth process of the seedlings, on one hand, the complete nutrient solution can be adopted to provide sufficient nutrition and moisture for the growth of plants, which is beneficial to ensuring the normal growth of the plants, and the nutrient content of the residual nutrient solution in a bucket is measured under the condition, so that the nutrient content and the nutrient absorption utilization rate required by the normal growth of the plants can be calculated; on the other hand, an incomplete nutrient solution is adopted to carry out a plant nutrient deficiency experiment, and then the deficient elements are supplemented, so that the plant nutrient deficiency symptom and the recovery process are observed; therefore, the normal growth environment or the nutrient deficiency growth environment of the plant can be simulated, the nutrient utilization efficiency of the normal growth of the plant can be further obtained, and the nutrient deficiency symptom of the plant can be known.
The invention also provides a method for measuring the absorption and utilization rate of plant nutrients, and the device adopting the technical scheme comprises the following steps:
a) placing the plant to be screened in fine sand of a flowerpot for cultivation and planting; adjusting environmental parameters according to the growth conditions of the plants to be screened, and then carrying out drip irrigation by adopting the nutrient solution in the hanging bottle to culture the plants;
b) detecting the concentration of each element in the initial nutrient solution, opening a valve after the plants are cultured for a certain time, collecting the residual nutrient solution in the flowerpot into a water bucket through a drain hole, detecting the concentration of each element in the residual nutrient solution, and calculating to obtain the absorption utilization rate of the plant nutrients.
Firstly, placing a plant to be screened in fine sand of a flowerpot for cultivation and planting; and adjusting environmental parameters according to the growth conditions of the plants to be screened, and then carrying out drip irrigation by adopting the nutrient solution in the hanging bottle to culture the plants. The present invention is not particularly limited in kind and source of the plant to be screened, and plants for determining nutrient absorption and utilization, which are well known to those skilled in the art, may be used. In a preferred embodiment of the present invention, the plant to be screened is American ginseng.
In the present invention, the environmental parameters preferably include temperature, humidity, illumination type and illumination intensity; in the preferred embodiment of the invention, the plant culture is carried out by adjusting the environmental parameters to 25 ℃ at room temperature, 60% of air humidity, and 3000Lux of red illumination according to the growth conditions of the American ginseng.
In the present invention, the flow rate of the drip irrigation is preferably 100mL/h to 300mL/h, more preferably 200 mL/h.
In the invention, in the process of plant cultivation, the dropping amount of the nutrient solution, namely the liquid depth at the bottom of the flowerpot, is preferably 1/5-1/3 of the height of the flowerpot; more preferably 1/4, which exceeds the height of the pot.
Then, the concentration of each element in the initial nutrient solution is detected, after the plants are cultured for a certain time, the valve is opened, the residual nutrient solution in the flowerpot is collected into the water bucket through the drain hole, the concentration of each element in the residual nutrient solution is detected, and the absorption utilization rate of the plant nutrients is calculated. In the present invention, the predetermined time is preferably 3d to 10d, and more preferably 7 d.
The invention realizes the determination of the absorption and utilization rate of plant nutrients based on sand culture treatment for the first time, and provides a specific sand culture device which can meet the requirement of regular detection on plants; the device and the method provided by the invention effectively simulate the real environment of plant planting in soil, are simpler and more convenient compared with the traditional water planting device in which the nutrient solution is frequently replaced, can avoid the occurrence of root rot, and have accurate determination results, thereby effectively mastering the nutrient absorption rule of plants and laying a foundation for scientific fertilization and management of farmland planting.
The invention provides a device and a method for measuring the absorption and utilization rate of plant nutrients; the device includes: a carrier; a plurality of flowerpots which are horizontally placed on the bearing frame; non-woven fabrics are laid in the flowerpot and filled with fine sand, and a drain hole with a valve is arranged at the position, close to the bottom, of the side wall; a bucket is hoisted on the drain hole; the hanging bottles are hung on one side of the bearing frame; nutrient solution is filled in the infusion bottle and is communicated with each flowerpot through an infusion pipeline; the infusion pipeline is provided with a flow rate control valve. Compared with the prior art, the method realizes the determination of the absorption and utilization rate of the plant nutrients based on the sand culture treatment for the first time, and provides the specific sand culture device which can meet the requirement of regular detection of the plant; the device and the method provided by the invention can avoid the occurrence of root rot, and the determination result is accurate, so that the nutrient absorption rule of the plant can be effectively mastered, and a foundation is laid for scientific fertilization and management of farmland planting.
In addition, the method for measuring the plant nutrient absorption and utilization rate is simple and rapid, and can also detect the plant leaf color change, the nutrient content in the plant body and the residual nutrient content of the nutrient solution, so that the plant nutrient absorption rule is mastered, the plant nutrient deficiency symptom is known, and the method has good popularization and application values.
To further illustrate the present invention, the following examples are provided for illustration.
Example 1
By adopting the device and the method for measuring the absorption and utilization rate of the plant nutrients in the technical scheme, the absorption and utilization rate of the nutrients (complete nutrient solution) of the American ginseng is measured:
the length, width and height of a bearing frame in the device are 3m multiplied by 1m multiplied by 2m, and a 5L water bucket, a 3.5L transfusion bottle and a 10L flowerpot are adopted; laying non-woven fabrics at the bottom of the flowerpot, filling 2/3 fine sand in the flowerpot, soaking the fine sand in 1.0mol/L hydrochloric acid for 3 days, and washing with distilled water to obtain a particle size of 0.5-1 mm; 3L of complete nutrient solution is filled in the hanging bottle, and the formula of the complete nutrient solution is as follows: 1.25g/L Ca (NO)3)2、0.12g/L FeSO4、37g/L MgSO4、28g/L(NH4)2HPO4、41g/L KNO3、0.6g/L H3BO3、0.4g/L MnSO4、0.004g/L CuSO4、0.004g/L ZnSO4pH5.5-6.5; the device is placed flat and facing the direction of the light in the greenhouse.
The method specifically comprises the following steps:
(1) placing annual American ginseng seedlings in flowerpot fine sand for cultivation and planting; regulating the environmental parameters to be 25 ℃ at room temperature, 60% of air humidity and 3000Lux according to the growth conditions of the American ginseng, and carrying out plant culture; controlling the flow rate of the transfusion bottle filled with the complete nutrient solution to be 200mL/h for drip irrigation; the amount of nutrient solution (the liquid depth at the bottom of the flowerpot) is required to exceed 1/4 of the height of the flowerpot.
(2) Detecting the concentration of each element in the initial nutrient solution, opening a valve of a flowerpot after seven days to discharge the residual nutrient solution into a bucket, detecting the concentration of each element in the residual nutrient solution in the bucket, and calculating the nutrient absorption utilization rate of the American ginseng for seven days; the results are shown in Table 1.
TABLE 1 nutrient absorption rate of American ginseng in example 1 of the present invention for seven days
Element(s) Initial concentration (mg/L) Concentration after seven days (mg/L) Nutrient absorption rate (%)
Phosphorus (P) 2786.89±1.66 2615.06±1.46 6.17±0.01
Potassium salt 4096.91±1.95 3798.94±2.02 7.27±0.02
Calcium carbonate 124.59±0.19 118.37±0.18 4.99±0
Magnesium alloy 3677.4±4.62 3524.51±5.05 4.16±0.02
Manganese oxide 39.47±0.14 38.22±0.13 3.16±0.01
Boron 59.62±0.04 58.81±0.06 1.36±0.03
Iron 11.89±0.01 11.51±0 3.2±0.02
Copper (Cu) 0.4±0 0.39±0 1.96±0
Zinc 0.4±0 0.39±0 2.44±0.04
Note: the data after "+/-" all represent the standard error of the data of three experiments.
Example 2
By adopting the device and the method for measuring the plant nutrient absorption and utilization rate in the technical scheme, the phosphorus deficiency experiment of the American ginseng is carried out:
the length, width and height of a bearing frame in the device are 3m multiplied by 1m multiplied by 2m, and 5L water buckets, 3.5L transfusion bottles multiplied by 2 and 10L flowerpots are adopted; laying non-woven fabrics at the bottom of the flowerpot, filling 2/3 fine sand in the flowerpot, soaking the fine sand in 1.0mol/L hydrochloric acid for 3 days, and washing with distilled water to obtain a particle size of 0.5-1 mm; one hanging bottle is filled with 3L of incomplete nutrient solution, and the formula of the incomplete nutrient solution is as follows: 1.25g/L Ca (NO)3)2、0.12g/L FeSO4、37g/L MgSO4、41g/L KNO3、0.6g/L H3BO3、0.4g/L MnSO4、0.004g/L CuSO4、0.004g/L ZnSO4And the pH value is 5.5-6.5, and 0.4L of 28g/L (NH) is filled in the other infusion bottle4)2HPO4A nutrient solution; the device is placed flat and facing the direction of the light in the greenhouse.
The method specifically comprises the following steps:
(1) placing annual American ginseng seedlings in flowerpot fine sand for cultivation and planting; regulating the environmental parameters to be 25 ℃ at room temperature, 60% of air humidity and 3000Lux according to the growth conditions of the American ginseng, and carrying out plant culture; firstly, drip irrigation is carried out on a hanging bottle filled with incomplete nutrient solution at the flow rate of 200mL/h, the process of phosphorus deficiency of American ginseng is observed for three days, and (NH) is filled in the hanging bottle on the fourth day4)2HPO4Drip irrigation is carried out by controlling the flow rate of a transfusion bottle of the nutrient solution to be 200 mL/h; the dropping amount of the nutrient solution (the liquid depth at the bottom of the flowerpot) needs to exceed 1/4 of the height of the flowerpot in the whole process.
(2) Detecting the concentration of each element in the initial nutrient solution, opening a valve of a flowerpot after seven days to discharge the residual nutrient solution into a bucket, detecting the concentration of each element in the residual nutrient solution in the bucket, and calculating the nutrient absorption utilization rate of the American ginseng for seven days; the results are shown in Table 2.
TABLE 2 nutrient absorption rate of American ginseng in example 2 of the present invention for seven days
Element(s) Initial concentration (mg/L) Concentration after seven days (mg/L) Nutrient absorption rate (%)
Phosphorus (P) 2792.2±3.64 2620.33±3.63 6.16±0.01
Potassium salt 4099.25±0.44 3801.94±0.43 7.25±0.01
Calcium carbonate 124.54±0.2 118.33±0.19 4.99±0
Magnesium alloy 3685.9±6.78 3532.51±6.01 4.16±0.02
Manganese oxide 39.44±0.14 38.18±0.15 3.21±0.04
Boron 59.57±0.19 58.77±0.18 1.35±0.02
Iron 11.93±0.04 11.55±0.03 3.18±0.03
Copper (Cu) 0.4±0 0.39±0 1.97±0.01
Zinc 0.4±0 0.39±0 2.42±0.01
Note: the data after "+/-" all represent the standard error of the data of three experiments.
From examples 1-2, the three parallel experiments are performed on the complete nutrient solution experiment and the incomplete nutrient solution experiment, the standard error of the experiment results is very small, the range is 0-6.78 mg/L, and the reliability of the detection results of the device and the method for determining the plant nutrient absorption and utilization rate is higher; meanwhile, the nutrient solution is convenient and simple to replace, and the residual liquid is collected without loss basically, so that simplicity, convenience and high efficiency are really achieved, and the nutrient solution can be popularized in the field of detection of the nutrient absorption rate of plants.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (10)

1. An apparatus for determining the absorption and utilization rate of nutrients in plants, comprising:
a carrier;
a plurality of flowerpots which are horizontally placed on the bearing frame; non-woven fabrics are laid in the flowerpot and filled with fine sand, and a drain hole with a valve is arranged at the position, close to the bottom, of the side wall; a bucket is hoisted on the drain hole;
the hanging bottles are hung on one side of the bearing frame; nutrient solution is filled in the infusion bottle and is communicated with each flowerpot through an infusion pipeline; the infusion pipeline is provided with a flow rate control valve.
2. The apparatus of claim 1, wherein the pot is made of PVC, has a volume of 5L to 15L, and is filled with fine sand in an amount of 1/2 to 4/5 of the height of the pot.
3. The device according to claim 1, wherein the fine sand is soaked in 0.5-1.5 mol/L hydrochloric acid for 2-4 days and then washed by water, and the particle size is 0.5-1 mm.
4. The apparatus according to claim 1, wherein the hole diameter of the drain hole is 1cm to 2 cm; the bucket is made of PVC and has a volume of 4-6L.
5. The device of claim 1, wherein the nutrient solution is selected from a complete nutrient solution or an incomplete nutrient solution.
6. The device according to claim 5, characterized in that said complete nutrient solution has a formula of:
1.25g/L Ca(NO3)2、0.12g/L FeSO4、37g/L MgSO4、28g/L(NH4)2HPO4、41g/LKNO3、0.6g/L H3BO3、0.4g/L MnSO4、0.004g/L CuSO4、0.004g/L ZnSO4、pH5.5~6.5。
7. a method for measuring the absorption and utilization rate of plant nutrients, which is characterized by adopting the device of any one of claims 1 to 6 and comprising the following steps:
a) placing the plant to be screened in fine sand of a flowerpot for cultivation and planting; adjusting environmental parameters according to the growth conditions of the plants to be screened, and then carrying out drip irrigation by adopting the nutrient solution in the hanging bottle to culture the plants;
b) detecting the concentration of each element in the initial nutrient solution, opening a valve after the plants are cultured for a certain time, collecting the residual nutrient solution in the flowerpot into a water bucket through a drain hole, detecting the concentration of each element in the residual nutrient solution, and calculating to obtain the absorption utilization rate of the plant nutrients.
8. The method according to claim 7, wherein the environmental parameters in step a) include temperature, humidity, illumination type and illumination intensity.
9. The method as claimed in claim 7, wherein the flow rate of the drip irrigation in the step a) is 100mL/h to 300 mL/h.
10. The method as claimed in claim 7, wherein the nutrient solution is dropped in an amount exceeding 1/4 of the height of the pot during the cultivation of the plant in the step a).
CN202010843060.5A 2020-08-20 2020-08-20 Device and method for measuring plant nutrient absorption and utilization rate Pending CN111937736A (en)

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