CN111920779A - Tremella fuciformis polysaccharide capsule medicament and preparation method thereof - Google Patents
Tremella fuciformis polysaccharide capsule medicament and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a tremella polysaccharide capsule medicament and a preparation method thereof, relating to the technical field of tremella polysaccharide, wherein the raw materials comprise: the preparation method of the tremella polysaccharide capsule medicament is realized by the following steps of 1: uniformly coating the tremella spore solution on a prepared inclined plane, and culturing for 5-7 days in a constant temperature room at 28 ℃; transferring the tremella polysaccharide to a seed shake flask mother bottle culture medium, putting the mother bottle culture medium into a constant temperature freezing oscillator, carrying out oscillation culture for 60-72h at the temperature of 28 ℃ and the rotation speed of 220-240rpm, carrying out propagation and fermentation on the tremella polysaccharide through a seed tank and a fermentation tank, and purifying the tremella polysaccharide through concentration, alcohol precipitation, centrifugation and vacuum drying processes, so that the drug effect of the tremella polysaccharide capsule is maximized, the drug effect of the tremella polysaccharide capsule is improved, and the tremella polysaccharide capsule is filled into capsules and packaged to obtain a finished product which can be stored for a long time.
Description
Technical Field
The invention relates to the technical field of tremella polysaccharide, in particular to a tremella polysaccharide capsule medicament and a preparation method thereof.
Background
Tremella is the fruiting body of fungi of the genus Tremella of the family Tremellaceae of the order Tremellales of the class Tremellales of the phylum Basidiomycota, also called Tremella, etc., and is known as the crown of fungi. Is an edible fungus with higher edible and medicinal value, and the research in recent years shows that: the tremella polysaccharide existing in tremella spore and sporophore is one kind of acidic heteropolysaccharide, and has several medicinal functions of raising immunity, resisting senility, inhibiting tumor synthesizing rate, promoting the synthesis of protein and nucleic acid, promoting the production of interferon, etc. and belongs to the field of functional oligose. The cultivation of tremella has been in China for over 100 years, but the tremella has the defects of long period, low yield, uneven tremella growing and single eating method. The production of tremella spore and mycelium by liquid submerged fermentation is carried out in large-scale fermentation tank without interference of environmental conditions, and by adjusting the composition of culture medium, selecting and controlling conditions of fermentation temperature, oxygen supply and the like, a large amount of tremella spore and mycelium can be obtained in a short time, and the effective components of the tremella spore and mycelium are basically the same as the tremella sporophore. The liquid culture method has the characteristics of large production scale, high yield, short period and easy realization of industrialization, the tremella is generally chrysanthemum-shaped or cockscomb-shaped, the diameter is 5-10 cm, the tremella is soft, white, semi-transparent and elastic, the tremella is taken as the traditional edible fungus in China and is historically a food which is deeply favored by the vast people, the tremella polysaccharide which is an active ingredient contained in the tremella polysaccharide has a special health care function, for example, the preparation process of the tremella polysaccharide soft capsule and the invention patent with the publication number of CN101439056A are designed to solve the problem that the hard capsule made of the tremella polysaccharide which is an active substance is easy to absorb moisture and deteriorate and also solve the problem that the syrup is inconvenient to quantify, the content is in a suspension state, the soft capsule is fast to absorb after being disintegrated, the content can be kept in a homogeneous state for a long time by the treatment method and the composition proportion of the content, but the preparation process of the existing tremella polysaccharide, the polysaccharide in the tremella can not be fully exerted, thereby reducing the drug effect of the tremella polysaccharide soft capsule.
Disclosure of Invention
The invention aims to solve the problems and designs a tremella polysaccharide capsule medicament and a preparation method thereof.
The technical scheme of the invention is that the tremella polysaccharide capsule medicament and the preparation method thereof, the raw materials comprise: the preparation method of the tremella polysaccharide capsule medicament is realized by the following steps:
step 1: uniformly coating the tremella spore solution on a prepared inclined plane, and culturing for 5-7 days in a constant temperature room at 28 ℃; transferring the strain to a seed shake flask mother bottle culture medium, placing the strain in a constant temperature freezing oscillator, carrying out shake culture for 60-72h at the temperature of 28 ℃ and the rotation speed of 220-plus-240 rpm, correspondingly inoculating each qualified seed shake flask mother bottle to a seed shake flask child bottle, one-to-one inoculating the seed shake flask mother bottle to the seed shake flask child bottle culture medium, placing the strain in the constant temperature freezing oscillator, carrying out shake culture for 48-60h at the temperature of 28 ℃ and the rotation speed of 100-plus-150 rpm;
step 2: weighing 2.4kg-2.5kg of glucose and 0.96kg-0.97kg of corn steep liquor dry powder as a first group, and weighing 40kg-45kg of glucose and 24kg-25kg of corn steep liquor dry powder as a second group;
and step 3: selecting a 500L seed tank and a 1000L fermentation tank for air elimination, and actually eliminating the seed tank and the fermentation tank after air elimination, taking 16% of the volume of the seed tank as the total volume of ingredients, sequentially adding 2.4kg-2.5kg of the first group of glucose and 0.96kg-0.97kg of corn steep liquor dry powder in the step 2 into the seed tank with 10% of water in the tank body under stirring, washing the seed tank with water to fill the raw material container, adding washing water into the tank, taking 80% of the volume of the fermentation tank as the total volume of the ingredients, sequentially adding 40kg-45kg of the second group of glucose and 24kg-25kg of corn steep liquor dry powder in the step 1 into the fermentation tank with 75% of water in the tank body under stirring, washing the raw material container with water to fill the washing water into the tank;
and 4, step 4: inoculating the seed tank in the step 3, closing a compressed air valve entering the seed tank when the pressure in the seed tank reaches 0.1Mpa, slightly opening an exhaust valve to slowly reduce the pressure in the tank, closing the exhaust valve when the pressure in the tank is reduced to 0.03Mpa, opening the compressed air valve to ensure that the compressed air pressure in the tank reaches 0.1Mpa, repeatedly operating, completely inoculating the tremella spores in the seed shake flask into the seed tank through the inoculation valve on the seed tank until the tremella seed liquid is completely pumped into the seed tank, and closing the inoculation valve;
and 5: the seeding tank inoculated in the step 4 is controlled to have the tank pressure of 0.03-0.05Mpa and the air flow rate of 2.4m3/h at the temperature of 27-29 ℃; stirring at a frequency of 46 Hz; culturing for 48 hours;
step 6: opening the inoculation valve of the seeding tank in the step 5, closing the inoculation valve to exhaust, increasing the pressure of the seeding tank to 0.1-0.15Mpa, then opening the inoculation valve of the fermentation tank, starting to transfer seeds, and closing the inoculation valve of the fermentation tank after the seed transfer is finished;
and 7: culturing the inoculated fermentation tank in the step 6 at the culture temperature of 27-29 ℃ and the tank pressure of 0.03-0.05Mpa at the air flow rate of 48m3/h and the stirring frequency of 35Hz for 72-90 hours to form fermentation liquor, heating to 80 ℃, and keeping for 30 minutes;
and 8: introducing the fermentation liquor obtained in the step 7 into a single-effect evaporation concentrator, carrying out first concentration under the conditions that the vacuum degree is controlled to be-0.06-0.09 MPa and the steam pressure is less than or equal to 0.3MPa, concentrating to the relative density of 1.08-1.15 (60 ℃), pumping into a spherical concentrator, carrying out second concentration under the conditions that the vacuum degree is controlled to be-0.06-0.09 MPa and the steam pressure is less than or equal to 0.3MPa, and concentrating to the relative density of 1.20-1.25 (60 ℃) to form concentrated solution;
and step 9: introducing the concentrated solution obtained in the step 8 into an alcohol precipitation tank, adding 2 times of ethanol into the alcohol precipitation tank to form a mixed solution, stirring, and standing for 8-12 hours after a precipitation layer is clear;
step 10: guiding the precipitate in the alcohol precipitation tank in the step 9 into a white steel barrel with a respirator, centrifuging for 30 minutes at the frequency of 42Hz, breaking the centrifuged wet paste into small pieces of 1cm3, and filling the small pieces into the white steel barrel;
step 11: step 10, putting the centrifuged wet paste into a vacuum drying oven in a split disc mode according to the thickness of 1.5-2.0cm per disc, and drying for 10-14 hours under the conditions that the heating circulating water temperature is set to be 85 ℃, the vacuum degree is-0.07-0.09 Mpa and the temperature is lower than 80 ℃ to form the tremella polysaccharide dry paste;
step 12: and (3) putting the dried tremella polysaccharide cream obtained in the step (11) into a Chinese herbal medicine grinder for grinding, filling the ground dried tremella polysaccharide cream into capsules by a capsule filling machine, polishing by a polishing machine, and finally packaging by an aluminum-plastic packaging machine to obtain finished products.
Preferably, the step 2 is performed by an empty tank sterilization process under the conditions that the pressure in the seed tank and the pressure in the fermentation tank are 0.1-0.2Mpa, the temperature is 120-.
Preferably, the actual sterilization in the step 3 is performed in the seed tank and the fermentation tank under the conditions of 0.1-0.2MPa of pressure, 120-123 ℃ of temperature and 30min of heat preservation time.
Preferably, after the sterilization of the seed tank and the fermentation tank is finished, the jacket cooling water valve is opened when the pressure of the seed tank and the fermentation tank is reduced to 0.03-0.05Mpa, and the temperature is reduced to 27-29 ℃.
Preferably, the temperature and pressure are monitored every two hours in step 5 and step 7.
Preferably, the temperature of the first concentration in the step 8 is controlled to be 60-80 ℃.
Preferably, the temperature of the second concentration in the step 8 is controlled to be 60-75 ℃.
Preferably, the ethanol concentration in the mixed solution in the step 9 reaches 65-70%.
Preferably, the wet paste after centrifugation in the step 10 is broken into small pieces of 1cm3 or chopped.
Preferably, the dried tremella polysaccharide paste crushed in the step 12 passes through a 80-mesh sieve.
Advantageous effects
The tremella polysaccharide capsule medicament prepared by the technical scheme of the invention and the preparation method thereof propagate and ferment tremella bacteria through the seed tank and the fermentation tank, and purify the tremella polysaccharide through the processes of concentration, alcohol precipitation, centrifugation and vacuum drying, thereby maximizing the drug effect of the tremella polysaccharide capsule, improving the drug effect of the tremella polysaccharide capsule, and filling and packaging the tremella polysaccharide capsule into finished products for long-term storage.
The tremella polysaccharide capsule is processed by modern preparation technology, not only retains the active ingredients and curative effect of tremella, but also exerts the characteristics of convenient taking and quick drug release of western medicine. The main component of the tremella polysaccharide capsule is tremella polysaccharide obtained by deep fermentation and separation and purification of tremella of Tremellaceae, the organism immunity function improves the function of leucocyte, can be used for leucopenia caused by chemo-treatment, radiotherapy and other reasons, the adjuvant treatment of chronic persistent hepatitis and chronic active hepatitis, and the tremella polysaccharide has obvious effect of inhibiting tumor growth on various animal tumor models.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1: a tremella polysaccharide capsule medicament and a preparation method thereof, the raw materials comprise: the preparation method of the tremella, glucose and corn steep liquor dry powder and tremella polysaccharide capsule medicament is realized by the following steps:
step 1: uniformly coating the tremella spore solution on a prepared inclined plane, and culturing for 5-7 days in a constant temperature room at 28 ℃; transferring the strain to a seed shake flask mother bottle culture medium, placing the strain in a constant temperature freezing oscillator, carrying out shake culture for 60-72h at the temperature of 28 ℃ and the rotation speed of 220-plus-240 rpm, correspondingly inoculating each qualified seed shake flask mother bottle to a seed shake flask child bottle, one-to-one inoculating the seed shake flask mother bottle to the seed shake flask child bottle culture medium, placing the strain in the constant temperature freezing oscillator, carrying out shake culture for 48-60h at the temperature of 28 ℃ and the rotation speed of 100-plus-150 rpm;
step 2: weighing 2.4kg of glucose and 0.96kg of corn steep liquor dry powder as a first group, and weighing 40kg of glucose and 24kg of corn steep liquor dry powder as a second group;
and step 3: selecting 500L of seed tanks and 1000L of fermentation tanks, performing empty tank sterilization under the conditions of pressure 0.1Mpa in the seed tanks and the fermentation tanks, temperature 120 ℃ and heat preservation time 30min, performing actual tank sterilization under the conditions of pressure 0.1Mpa in the seed tanks and the fermentation tanks, temperature 120 ℃ and heat preservation time 30min, taking 16% of the volume of the seed tanks as the total volume of ingredients, adding 2.4kg of the first group of glucose and 0.96kg of corn steep liquor dry powder in the step 2 into the seed tanks with 10% of water in the tank body under stirring, washing the raw material containers with water, adding washing water into the tanks, taking 80% of the volume of the fermentation tanks as the total volume of ingredients, adding 40kg of the second group of glucose and 24kg of the corn steep liquor in the step 1 into the fermentation tanks with 75% of water in the tank body under stirring, washing the raw material container with water, adding the washing water into the tank, sterilizing the solid tank, and opening the jacket cooling water valve when the pressure of the seed tank and the fermentation tank is reduced to 0.03Mpa to reduce the temperature to 27 ℃;
and 4, step 4: inoculating the seed tank in the step 3, closing a compressed air valve entering the seed tank when the pressure in the seed tank reaches 0.1Mpa, slightly opening an exhaust valve to slowly reduce the pressure in the tank, closing the exhaust valve when the pressure in the tank is reduced to 0.03Mpa, opening the compressed air valve to ensure that the compressed air pressure in the tank reaches 0.1Mpa, repeatedly operating, completely inoculating the Tremella fuciformis into the seed tank through the inoculation valve on the seed tank until the Tremella fuciformis is completely pumped into the seed tank, and closing the inoculation valve;
and 5: the seeding tank inoculated in the step 4 is maintained at 27 ℃,the pressure of the tank is controlled to be 0.03MPa, and the air flow rate is 2.4m3H; stirring at a frequency of 46 Hz; culturing for 48 hours, and monitoring the temperature and the pressure once every two hours;
step 6: opening the inoculation valve of the seeding tank in the step 5, closing the inoculation valve to exhaust, increasing the pressure of the seeding tank to 0.1Mpa, then opening the inoculation valve of the fermentation tank, starting to transfer seeds, and closing the inoculation valve of the fermentation tank after the seed transfer is finished;
and 7: the fermentation tank inoculated in the step 6 is cultured at the temperature of 27 ℃, the tank pressure is controlled to be 0.03Mpa, and the air flow rate is 48m3H, stirring at the frequency of 35Hz, culturing for 72 hours to form a fermentation liquid, monitoring the temperature and the pressure once every two hours, heating to 80 ℃, keeping for 30 minutes, and inactivating;
and 8: introducing the fermentation liquor obtained in the step 7 into a single-effect evaporation concentrator, performing first concentration under the conditions that the vacuum degree is controlled to be-0.06 MPa, the steam pressure is less than or equal to 0.3MPa and the temperature is controlled to be 60 ℃, concentrating to the relative density of 1.08-1.15 (60 ℃), pumping into a spherical concentrator, performing second concentration under the conditions that the vacuum degree is controlled to be-0.06 MPa, the steam pressure is less than or equal to 0.3MPa and the temperature is controlled to be 60 ℃, and concentrating to the relative density of 1.20-1.25 (60 ℃) to form concentrated solution;
and step 9: introducing the concentrated solution obtained in the step 8 into an alcohol precipitation tank, adding 2 times of ethanol into the alcohol precipitation tank to form a mixed solution, starting stirring when the concentration of the ethanol in the mixed solution reaches 65%, stopping stirring after stirring for 20 minutes, standing for 0.5 hour, observing the state in the alcohol precipitation tank, adding the ethanol until a clear precipitation layer is formed, and standing for 8 hours after the precipitation layer is clear;
step 10: guiding the precipitate in the alcohol precipitation tank in the step 9 into a white steel barrel with a respirator, centrifuging for 30 minutes at the frequency of 42Hz, and breaking the centrifuged wet paste into 1cm3Cutting into small pieces, and placing into a white steel barrel;
step 11: loading the centrifuged wet paste in the step 10 into a vacuum drying oven in a split disc mode according to the thickness of 1.5cm per disc, and drying for 10 hours under the conditions that the heating circulating water temperature is set to be 85 ℃, the vacuum degree is-0.07 Mpa and the temperature is lower than 80 ℃ to form the tremella polysaccharide dry paste;
step 12: and (3) putting the dried tremella polysaccharide cream obtained in the step (11) into a Chinese herbal medicine grinder for grinding, enabling all the ground dried tremella polysaccharide cream to pass through a 80-mesh sieve, putting the dried tremella polysaccharide cream into a batch mixer, operating at the rotating speed of 12rpm/m for 20 minutes, filling the mixed dried tremella polysaccharide cream powder into capsules by a capsule filling machine, polishing the capsules by a polishing machine, and finally packaging the capsules by an aluminum-plastic packaging machine to obtain the finished product.
Example 2: a tremella polysaccharide capsule medicament and a preparation method thereof, the raw materials comprise: the preparation method of the tremella, glucose and corn steep liquor dry powder and tremella polysaccharide capsule medicament is realized by the following steps:
step 1: uniformly coating the tremella spore solution on a prepared inclined plane, and culturing for 5-7 days in a constant temperature room at 28 ℃; transferring the strain to a seed shake flask mother bottle culture medium, placing the strain in a constant temperature freezing oscillator, carrying out shake culture for 60-72h at the temperature of 28 ℃ and the rotation speed of 220-plus-240 rpm, correspondingly inoculating each qualified seed shake flask mother bottle to a seed shake flask child bottle, one-to-one inoculating the seed shake flask mother bottle to the seed shake flask child bottle culture medium, placing the strain in the constant temperature freezing oscillator, carrying out shake culture for 48-60h at the temperature of 28 ℃ and the rotation speed of 100-plus-150 rpm;
step 2: weighing 2.5kg of glucose and 0.97kg of corn steep liquor dry powder as a first group, and weighing 45kg of glucose and 25kg of corn steep liquor dry powder as a second group;
and step 3: selecting 500L of seed tanks and 1000L of fermentation tanks, performing empty tank sterilization under the conditions of pressure 0.2Mpa in the seed tanks and the fermentation tanks, temperature 140 ℃ and heat preservation time 30min, performing actual tank sterilization under the conditions of pressure 0.2Mpa in the seed tanks and the fermentation tanks, temperature 123 ℃ and heat preservation time 30min, taking 16% of the volume of the seed tanks as the total volume of ingredients, adding 2.5kg of the first group of glucose and 0.97kg of corn steep liquor dry powder in the step 2 into the seed tanks with 10% of water in the tank body under stirring, washing the raw material containers with water, adding washing water into the tanks, taking 80% of the volume of the fermentation tanks as the total volume of ingredients, adding 45kg of the second group of glucose and 25kg of the corn steep liquor in the step 1 into the fermentation tanks with 75% of water in the tank body under stirring, washing the raw material container with water, adding the washing water into the tank, sterilizing the solid tank, and opening the jacket cooling water valve when the pressure of the seed tank and the fermentation tank is reduced to 0.05Mpa to reduce the temperature to 29 ℃;
and 4, step 4: inoculating the seed tank in the step 3, closing a compressed air valve entering the seed tank when the pressure in the seed tank reaches 0.1Mpa, slightly opening an exhaust valve to slowly reduce the pressure in the tank, closing the exhaust valve when the pressure in the tank is reduced to 0.03Mpa, opening the compressed air valve to ensure that the compressed air pressure in the tank reaches 0.1Mpa, repeatedly operating, completely inoculating the Tremella fuciformis into the seed tank through the inoculation valve on the seed tank until the Tremella fuciformis is completely pumped into the seed tank, and closing the inoculation valve;
and 5: the seeding tank inoculated in the step 4 is controlled at 29 ℃, the tank pressure is controlled to be 0.05Mpa, and the air flow rate is 2.4m3H; stirring at a frequency of 46 Hz; culturing for 48 hours, and monitoring the temperature and the pressure once every two hours;
step 6: opening the inoculation valve of the seeding tank in the step 5, closing the inoculation valve to exhaust, increasing the pressure of the seeding tank to 0.15Mpa, then opening the inoculation valve of the fermentation tank, starting to transfer seeds, and closing the inoculation valve of the fermentation tank after the seed transfer is finished;
and 7: the fermentation tank inoculated in the step 6 is cultured at the temperature of 29 ℃, the tank pressure is controlled to be 0.05Mpa, and the air flow rate is 48m3H, stirring at the frequency of 35Hz, culturing for 90 hours to form a fermentation liquid, monitoring the temperature and the pressure once every two hours, heating to 80 ℃, keeping for 30 minutes, and inactivating;
and 8: introducing the fermentation liquor obtained in the step 7 into a single-effect evaporation concentrator, performing first concentration under the conditions that the vacuum degree is controlled to be-0.09 MPa, the steam pressure is less than or equal to 0.3MPa and the temperature is controlled to be 60 ℃, concentrating to the relative density of 1.08-1.15 (60 ℃), pumping into a spherical concentrator, performing second concentration under the conditions that the vacuum degree is controlled to be-0.09 MPa, the steam pressure is less than or equal to 0.3MPa and the temperature is controlled to be 60 ℃, and concentrating to the relative density of 1.20-1.25 (60 ℃) to form concentrated solution;
and step 9: introducing the concentrated solution obtained in the step 8 into an alcohol precipitation tank, adding 2 times of ethanol into the alcohol precipitation tank to form a mixed solution, stirring until the concentration of the ethanol in the mixed solution reaches more than 70%, stirring for 20 minutes, stopping stirring, standing for 0.5 hour, observing the state in the alcohol precipitation tank, adding ethanol until a clear precipitation layer is formed, and standing for 12 hours after the precipitation layer is clear;
step 10: guiding the precipitate in the alcohol precipitation tank in the step 9 into a white steel barrel with a respirator, centrifuging for 30 minutes at the frequency of 42Hz, and breaking the centrifuged wet paste into 1cm3Cutting into small pieces, and placing into a white steel barrel;
step 11: loading the centrifuged wet paste in the step 10 into a vacuum drying oven in a split disc mode according to the thickness of 2.0cm per disc, and drying for 14 hours under the conditions that the heating circulating water temperature is set to be 85 ℃, the vacuum degree is 0.09Mpa and the temperature is lower than 80 ℃ to form the tremella polysaccharide dry paste;
step 12: and (3) putting the dried tremella polysaccharide cream obtained in the step (11) into a Chinese herbal medicine grinder for grinding, enabling all the ground dried tremella polysaccharide cream to pass through a 80-mesh sieve, putting the dried tremella polysaccharide cream into a batch mixer, operating at the rotating speed of 12rpm/m for 20 minutes, filling the mixed dried tremella polysaccharide cream powder into capsules by a capsule filling machine, polishing the capsules by a polishing machine, and finally packaging the capsules by an aluminum-plastic packaging machine to obtain the finished product.
The technical solutions described above only represent the preferred technical solutions of the present invention, and some possible modifications to some parts of the technical solutions by those skilled in the art all represent the principles of the present invention, and fall within the protection scope of the present invention.
Claims (10)
1. The tremella polysaccharide capsule medicament and the preparation method thereof are characterized in that the raw materials comprise: the preparation method of the tremella polysaccharide capsule medicament is realized by the following steps:
step 1: uniformly coating the tremella spore solution on a prepared inclined plane, and culturing for 5-7 days in a constant temperature room at 28 ℃; transferring the strain to a seed shake flask mother bottle culture medium, placing the strain in a constant temperature freezing oscillator, carrying out shake culture for 60-72h at the temperature of 28 ℃ and the rotation speed of 220-plus-240 rpm, correspondingly inoculating each qualified seed shake flask mother bottle to a seed shake flask child bottle, one-to-one inoculating the seed shake flask mother bottle to the seed shake flask child bottle culture medium, placing the strain in the constant temperature freezing oscillator, carrying out shake culture for 48-60h at the temperature of 28 ℃ and the rotation speed of 100-plus-150 rpm;
step 2: weighing 2.4kg-2.5kg of glucose and 0.96kg-0.97kg of corn steep liquor dry powder as a first group, and weighing 40kg-45kg of glucose and 24kg-25kg of corn steep liquor dry powder as a second group;
and step 3: selecting a 500L seed tank and a 1000L fermentation tank for air elimination, and actually eliminating the seed tank and the fermentation tank after air elimination, taking 16% of the volume of the seed tank as the total volume of ingredients, sequentially adding 2.4kg-2.5kg of the first group of glucose and 0.96kg-0.97kg of corn steep liquor dry powder in the step 2 into the seed tank with 10% of water in the tank body under stirring, washing the seed tank with water to fill the raw material container, adding washing water into the tank, taking 80% of the volume of the fermentation tank as the total volume of the ingredients, sequentially adding 40kg-45kg of the second group of glucose and 24kg-25kg of corn steep liquor dry powder in the step 1 into the fermentation tank with 75% of water in the tank body under stirring, washing the raw material container with water to fill the washing water into the tank;
and 4, step 4: inoculating the seed tank in the step 3, closing a compressed air valve entering the seed tank when the pressure in the seed tank reaches 0.1Mpa, slightly opening an exhaust valve to slowly reduce the pressure in the tank, closing the exhaust valve when the pressure in the tank is reduced to 0.03Mpa, opening the compressed air valve to ensure that the compressed air pressure in the tank reaches 0.1Mpa, repeatedly operating, completely inoculating the tremella spores in the seed shake flask into the seed tank through the inoculation valve on the seed tank until the tremella seed liquid is completely pumped into the seed tank, and closing the inoculation valve;
and 5: the seeding tank inoculated in the step 4 is controlled at 27-29 ℃, the tank pressure is controlled at 0.03-0.05Mpa, and the air flow rate is 2.4m3H; stirring at a frequency of 46 Hz; culturing for 48 hours;
step 6: opening the inoculation valve of the seeding tank in the step 5, closing the inoculation valve to exhaust, increasing the pressure of the seeding tank to 0.1-0.15Mpa, then opening the inoculation valve of the fermentation tank, starting to transfer seeds, and closing the inoculation valve of the fermentation tank after the seed transfer is finished;
and 7: the fermentation tank inoculated in the step 6 has the culture temperature of 27-29 ℃, the tank pressure of 0.03-0.05Mpa and the air flow rate of 48m3H, stirring at the frequency of 35Hz, culturing for 72-90 hours to form fermentation liquor, heating to 80 ℃, and keeping for 30 minutes;
and 8: introducing the fermentation liquor obtained in the step 7 into a single-effect evaporation concentrator, carrying out first concentration under the conditions that the vacuum degree is controlled to be-0.06-0.09 MPa and the steam pressure is less than or equal to 0.3MPa, concentrating to the relative density of 1.08-1.15 (60 ℃), pumping into a spherical concentrator, carrying out second concentration under the conditions that the vacuum degree is controlled to be-0.06-0.09 MPa and the steam pressure is less than or equal to 0.3MPa, and concentrating to the relative density of 1.20-1.25 (60 ℃) to form concentrated solution;
and step 9: introducing the concentrated solution obtained in the step 8 into an alcohol precipitation tank, adding 2 times of ethanol into the alcohol precipitation tank to form a mixed solution, stirring, and standing for 8-12 hours after a precipitation layer is clear;
step 10: guiding the precipitate in the alcohol precipitation tank in the step 9 into a white steel barrel with a respirator, centrifuging for 30 minutes at the frequency of 42Hz, and breaking the centrifuged wet paste into 1cm3The small blocks are put into a white steel barrel;
step 11: step 10, putting the centrifuged wet paste into a vacuum drying oven in a split disc mode according to the thickness of 1.5-2.0cm per disc, and drying for 10-14 hours under the conditions that the heating circulating water temperature is set to be 85 ℃, the vacuum degree is-0.07-0.09 Mpa and the temperature is lower than 80 ℃ to form the tremella polysaccharide dry paste;
step 12: and (3) putting the dried tremella polysaccharide cream obtained in the step (11) into a Chinese herbal medicine grinder for grinding, filling the ground dried tremella polysaccharide cream into capsules by a capsule filling machine, polishing by a polishing machine, and finally packaging by an aluminum-plastic packaging machine to obtain finished products.
2. The tremella polysaccharide capsule as claimed in claim 1, wherein the sterilization in step 2 is performed under the conditions of 0.1-0.2Mpa pressure, 120-140 ℃ temperature, and 30min holding time in the seeding tank and the fermentation tank.
3. The tremella polysaccharide capsule as claimed in claim 1, wherein the sterilization in step 3 is performed in seed tank and fermentation tank under 0.1-0.2MPa, 120-123 deg.C, and 30min holding time.
4. The tremella polysaccharide capsule as claimed in claim 3, wherein the temperature of the seed tank and the fermenter is lowered to 27-29 deg.C by opening the jacket cooling water valve when the pressure in the seed tank and the fermenter is lowered to 0.03-0.05MPa after the sterilization of the fermenter is completed.
5. The tremella polysaccharide capsule as claimed in claim 1, wherein the temperature and pressure are monitored every two hours in steps 5 and 7.
6. The tremella polysaccharide capsule as claimed in claim 1, wherein the temperature of the first concentration in step 8 is controlled to 60-80 ℃.
7. The tremella polysaccharide capsule as claimed in claim 1, wherein the temperature of the second concentration in step 8 is controlled to 60-75 ℃.
8. The tremella polysaccharide capsule as claimed in claim 1, wherein the ethanol concentration in the mixture of step 9 is 65% -70%.
9. The tremella polysaccharide capsule as claimed in claim 1, wherein the wet paste centrifuged in step 10 is broken into 1cm3Or chopped.
10. The tremella polysaccharide capsule as claimed in claim 1, wherein the dried extract of tremella polysaccharide obtained by pulverizing in step 12 is passed through a 80 mesh sieve.
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| CN101439056A (en) * | 2008-12-18 | 2009-05-27 | 北京九和药业有限公司 | Technique for preparing Tremella fuciformis spores polysaccharide soft capsules |
| CN101990822A (en) * | 2009-08-20 | 2011-03-30 | 吴访其 | Tremella spore fermentation material bioactive feed additive |
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| CN110964761A (en) * | 2019-12-13 | 2020-04-07 | 江苏远大仙乐药业有限公司 | Tremella and application thereof |
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