CN111918964A - 促进免疫细胞增殖的方法 - Google Patents

促进免疫细胞增殖的方法 Download PDF

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CN111918964A
CN111918964A CN201980022452.0A CN201980022452A CN111918964A CN 111918964 A CN111918964 A CN 111918964A CN 201980022452 A CN201980022452 A CN 201980022452A CN 111918964 A CN111918964 A CN 111918964A
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acid sequence
amino acid
seq
homology
immune cell
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凌有国
郭昊
马海立
李慧姣
王华菁
应碧
杨焕凤
何晓文
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Shanghai OriginCell Medical Technology Co Ltd
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Abstract

本申请公开了一种促进免疫细胞增殖的方法。所述方法包括以下步骤:使该免疫细胞中低密度脂蛋白受体相关蛋白或其片段的表达量上调。

Description

PCT国内申请,说明书已公开。

Claims (154)

  1. PCT国内申请,权利要求书已公开。
CN201980022452.0A 2018-03-26 2019-03-25 促进免疫细胞增殖的方法 Pending CN111918964A (zh)

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CN2018102518757 2018-03-26
CN201810251875 2018-03-26
PCT/CN2019/079576 WO2019184886A1 (zh) 2018-03-26 2019-03-25 促进免疫细胞增殖的方法

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CN111918964A true CN111918964A (zh) 2020-11-10

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EP (1) EP3778876A4 (zh)
JP (1) JP2021518149A (zh)
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WO (1) WO2019184886A1 (zh)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114891115A (zh) * 2022-02-18 2022-08-12 杭州美中疾病基因研究院有限公司 一种car细胞疗法用核酸构建体及其慢病毒载体、细胞制剂和应用
WO2022228429A1 (zh) * 2021-04-27 2022-11-03 原启生物科技(上海)有限责任公司 靶向bcma的嵌合抗原受体及其应用
CN116656614A (zh) * 2022-02-17 2023-08-29 杭州乾合细胞生物科技有限公司 一种靶向Her-2的CAR-NK细胞及其应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016008973A1 (en) * 2014-07-16 2016-01-21 Universitaet Zu Koeln Chimeric antigen receptor and its use
TW201729818A (zh) * 2015-10-20 2017-09-01 凱特製藥公司 製備用於t細胞治療之t細胞的方法
WO2018009972A1 (en) * 2016-07-14 2018-01-18 Peter Maccallum Cancer Institute Chimeric antigen receptor modified t cells
CN108392492A (zh) * 2017-12-28 2018-08-14 中国人民解放军第二军医大学东方肝胆外科医院 Ldlr过表达在nk细胞过继治疗中的应用
US20200179510A1 (en) * 2017-03-24 2020-06-11 Yale University Low density lipoprotein receptor related protein 5 inhibition suppresses tumor formation

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113429455A (zh) * 2015-08-28 2021-09-24 赛品制药有限责任公司 用于疾病治疗的方法
GB201516972D0 (en) * 2015-09-25 2015-11-11 Epiontis Gmbh LRP5 as epigenetic marker for the identification of immune cells, in particular B-cells

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016008973A1 (en) * 2014-07-16 2016-01-21 Universitaet Zu Koeln Chimeric antigen receptor and its use
TW201729818A (zh) * 2015-10-20 2017-09-01 凱特製藥公司 製備用於t細胞治療之t細胞的方法
WO2018009972A1 (en) * 2016-07-14 2018-01-18 Peter Maccallum Cancer Institute Chimeric antigen receptor modified t cells
US20200179510A1 (en) * 2017-03-24 2020-06-11 Yale University Low density lipoprotein receptor related protein 5 inhibition suppresses tumor formation
CN108392492A (zh) * 2017-12-28 2018-08-14 中国人民解放军第二军医大学东方肝胆外科医院 Ldlr过表达在nk细胞过继治疗中的应用

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
J ZHANG等: "Wnt signaling activation and mammary gland hyperplasia in MMTV-LRP6 transgenic mice: implication for breast cancer tumorigenesis", ONCOGENE, vol. 29, no. 4, 28 January 2010 (2010-01-28), pages 539, XP037742491, DOI: 10.1038/onc.2009.339 *
PING HE等: "Co-expressing LRP6 With Anti-CD19 CAR-T Cells for Improved Therapeutic Effect Against B-ALL", 《FRONTIERS IN ONCOLOGY》, vol. 10, 15 September 2020 (2020-09-15), pages 1 - 9 *
于田田;李敬达;刘庆平;王仁军;乔辉;陈玉华;李明英;于柯楠;迟彦;: "人血凝素样氧化型低密度脂蛋白受体1双顺反子表达载体的构建及其在293T细胞中的表达", 《中国动脉硬化杂志》, vol. 25, no. 01, 21 January 2017 (2017-01-21), pages 25 - 31 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022228429A1 (zh) * 2021-04-27 2022-11-03 原启生物科技(上海)有限责任公司 靶向bcma的嵌合抗原受体及其应用
CN116656614A (zh) * 2022-02-17 2023-08-29 杭州乾合细胞生物科技有限公司 一种靶向Her-2的CAR-NK细胞及其应用
CN114891115A (zh) * 2022-02-18 2022-08-12 杭州美中疾病基因研究院有限公司 一种car细胞疗法用核酸构建体及其慢病毒载体、细胞制剂和应用

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US12005080B2 (en) 2024-06-11
EP3778876A1 (en) 2021-02-17
US20210106620A1 (en) 2021-04-15
WO2019184886A1 (zh) 2019-10-03
JP2021518149A (ja) 2021-08-02
EP3778876A4 (en) 2022-01-12

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