CN111909282A - Method for extracting cyperus esculentus polysaccharide - Google Patents
Method for extracting cyperus esculentus polysaccharide Download PDFInfo
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- CN111909282A CN111909282A CN201910375309.1A CN201910375309A CN111909282A CN 111909282 A CN111909282 A CN 111909282A CN 201910375309 A CN201910375309 A CN 201910375309A CN 111909282 A CN111909282 A CN 111909282A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
A method for extracting cyperus esculentus polysaccharide comprises the following steps: crushing the puffed cyperus esculentus, and sieving to obtain cyperus esculentus powder for later use; adding distilled water into the cyperus esculentus powder according to the liquid-material ratio of 10-20 times, and extracting soluble total sugar under the ultrasonic condition to obtain cyperus esculentus soluble total sugar solution; centrifuging the cyperus esculentus soluble total sugar solution, and collecting supernatant; concentrating the supernatant under reduced pressure to a preset volume, adding ethanol while stirring to generate a mixed solution, standing the mixed solution, centrifuging, and collecting precipitates in the mixed solution through alcohol precipitation; and after removing residual ethanol in the precipitate, re-dissolving the precipitate after ethanol removal by using distilled water, and performing vacuum freeze drying to obtain the cyperus esculentus crude polysaccharide.
Description
Technical Field
The invention relates to an agricultural product processing technology, in particular to a method for efficiently extracting cyperus esculentus polysaccharide.
Background
Cyperus esculentus belongs to angiosperma, monocotyledonous plant class, Cyperales, Cyperaceae and Cyperus, is native to African Nile river basin, is also called underground walnut and tiger nut, and enjoys the reputation of 'fruit of life'. Is a novel multipurpose crop integrating oil collection, grain, grazing and feeding, which has high quality, high yield and high comprehensive utilization value. Cyperus esculentus has been successfully and widely planted in tropical, subtropical and temperate regions. The cyperus esculentus does not contain gluten protein, is not easy to cause allergy, and is a high-quality raw material for producing formula food and special diet. Since the introduction of the original Su Union and the successful cultivation by the institute of plants of Chinese academy of sciences in 1952, more than 20 provinces are planted in the north of China from Heilongjiang, inner Mongolia and Xinjiang, south to Yunnan, Guangxi and Guangdong.
The cyperus esculentus is rich in nutritional ingredients, wherein the oil content is usually 20% -30%, the starch content is 25% -40%, the sugar content is 15% -20%, the crude protein content is about 8%, the unsaturated fatty acid content is more than 80%, the oleic acid content is 65%, the linoleic acid content is 15%, and the cyperus esculentus is rich in vitamin E and various trace mineral elements.
Cyperus esculentus is an important oil crop, and oil extraction is an effective means for utilization at present, while development and utilization of other ingredients such as polysaccharide are relatively less.
Disclosure of Invention
The invention aims to solve the technical problem of providing a method for efficiently extracting the cyperus esculentus polysaccharide so as to realize high-value utilization of the cyperus esculentus.
In order to achieve the above object, the present invention provides a method for extracting cyperus esculentus polysaccharide, comprising the steps of:
s100, crushing the puffed cyperus esculentus, and sieving to obtain cyperus esculentus powder for later use;
s200, adding distilled water into the cyperus esculentus powder according to the liquid-material ratio of 10-20 times, and extracting soluble total sugar under the ultrasonic condition to obtain cyperus esculentus soluble total sugar solution;
s300, centrifuging the cyperus esculentus soluble total sugar solution, and collecting supernatant;
s400, concentrating the supernatant under reduced pressure to a preset volume, adding ethanol while stirring to generate a mixed solution, standing the mixed solution, centrifuging, and collecting precipitates in the mixed solution through alcohol precipitation; and
s500, after removing residual ethanol in the precipitate, re-dissolving the precipitate after removing the ethanol with distilled water, and performing vacuum freeze drying to obtain the cyperus esculentus crude polysaccharide.
The method for extracting the cyperus esculentus polysaccharide is characterized in that the particle size and the mesh number of the cyperus esculentus powder are 80-120 meshes.
The method for extracting the cyperus esculentus polysaccharide is characterized in that the particle size of the cyperus esculentus powder is 100 meshes.
The method for extracting the cyperus esculentus polysaccharide comprises the steps of performing ultrasonic treatment on the cyperus esculentus polysaccharide at the ultrasonic temperature of 50-80 ℃, performing ultrasonic treatment at the ultrasonic power of 200-500W, and performing ultrasonic treatment for 5-10 min.
The method for extracting the cyperus esculentus polysaccharide comprises the steps of performing centrifugation at the rotation speed of the centrifuge of 5000-.
The method for extracting the cyperus esculentus polysaccharide comprises the following steps of: adding ethanol while stirring until the volume fraction is 75% v/v, standing at 4 ℃ for more than 12h, centrifuging at the rotation speed of 2500-3500r/min for 15min, and collecting the precipitate.
In the method for extracting the cyperus esculentus polysaccharide, in the step S500, the precipitate is subjected to removal of residual ethanol to obtain a dried substance, distilled water is added into the dried substance according to the liquid-material ratio of 5-20mL/g to obtain a re-dissolved substance, and the re-dissolved substance is subjected to vacuum freeze drying to obtain the cyperus esculentus crude polysaccharide.
In the method for extracting the cyperus esculentus polysaccharide, in the step S100, the puffed cyperus esculentus is crushed and then passes through a 100-mesh sieve to obtain the cyperus esculentus powder, and the cyperus esculentus powder is stored in a ventilated and dried place for later use.
The invention has the technical effects that:
the invention can realize the high-efficiency and value-added utilization of the cyperus esculentus, can drive the health and high-value development of the industry, can obtain active ingredients with higher economic value, and further realizes the development of functional products of the cyperus esculentus.
The present invention will be described in detail with reference to specific examples, but the present invention is not limited thereto.
Detailed Description
The following is a detailed description of the structural and operational principles of the present invention:
the method for extracting the cyperus esculentus polysaccharide comprises the following steps of:
step S100, crushing the puffed cyperus esculentus, and sieving to obtain cyperus esculentus powder for later use, wherein the particle size of the cyperus esculentus powder is 80-120 meshes, preferably 100 meshes, for example, the puffed cyperus esculentus can be crushed and then sieved by a 100-mesh sieve to obtain the cyperus esculentus powder, and the cyperus esculentus powder is placed in a ventilation and drying place for later use;
step S200, adding distilled water into the cyperus esculentus powder according to the liquid-material ratio of 10-20 times, and extracting soluble total sugar under the ultrasonic wave condition to obtain cyperus esculentus soluble total sugar solution; wherein the ultrasonic conditions are preferably that the ultrasonic temperature is 50-80 ℃, the ultrasonic power is 200-500W, and the ultrasonic time is 5-10 min;
step S300, centrifuging the cyperus esculentus soluble total sugar solution, and collecting supernatant, wherein the conditions of the centrifugation are preferably that the rotating speed of a centrifuge is 5000-;
step S400, concentrating the supernate under reduced pressure to a certain preset volume, adding ethanol while stirring to generate a mixed solution, standing the mixed solution, performing centrifugal treatment, and collecting precipitates in the mixed solution through alcohol precipitation, wherein the alcohol precipitation comprises the following steps: adding ethanol while stirring until the volume fraction is 75% v/v, standing at 4 ℃ for more than 12h, then centrifuging at the rotation speed of 2500-;
and S500, after removing residual ethanol in the precipitate, redissolving the precipitate after the ethanol removal by using distilled water, and performing vacuum freeze drying to obtain the cyperus esculentus crude polysaccharide, wherein the precipitate is subjected to the removal of the residual ethanol to obtain a dried substance, the dried substance is added with the distilled water according to the liquid-material ratio of 5-20mL/g to obtain a redissolved substance, and the redissolved substance is subjected to vacuum freeze drying to obtain the cyperus esculentus crude polysaccharide.
The process of the invention for extracting cyperus esculentus polysaccharide is illustrated below in a specific example:
s100, crushing the puffed cyperus esculentus, sieving the crushed cyperus esculentus with a 100-mesh sieve to obtain cyperus esculentus powder, and storing the cyperus esculentus powder in a ventilated and dry place for later use;
s200, adding 500mL of distilled water into 50g of cyperus esculentus powder, and extracting soluble total sugar solution under the conditions that the temperature is 60 ℃, the ultrasonic power is 300W, and the ultrasonic time is 10 min;
s300, centrifuging the extracted soluble total sugar solution for 15min at the rotating speed of a centrifugal machine of 6000r/min, and collecting supernatant;
s400, concentrating the collected supernatant under reduced pressure to a certain volume, adding ethanol while stirring until the volume fraction is 75% (v/v), standing for 13h at 4 ℃, centrifuging for 15min at the rotating speed of a centrifuge of 3000r/min, and collecting precipitates;
s500, removing residual ethanol from the precipitate to obtain a dried substance, adding distilled water into the dried substance according to the liquid-material ratio of 10mL/g for redissolution, and performing vacuum freeze drying on the redissolved substance to obtain the cyperus esculentus crude polysaccharide.
The invention can realize the high-efficiency and value-added utilization of the cyperus esculentus, can drive the health and high-value development of the industry, can obtain active ingredients with higher economic value, and further realizes the development of functional products of the cyperus esculentus.
The present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof, and it should be understood that various changes and modifications can be effected therein by one skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (10)
1. The method for extracting the cyperus esculentus polysaccharide is characterized by comprising the following steps of:
s100, crushing the puffed cyperus esculentus, and sieving to obtain cyperus esculentus powder for later use;
s200, adding distilled water into the cyperus esculentus powder according to the liquid-material ratio of 10-20 times, and extracting soluble total sugar under the ultrasonic condition to obtain cyperus esculentus soluble total sugar solution;
s300, centrifuging the cyperus esculentus soluble total sugar solution, and collecting supernatant;
s400, concentrating the supernatant under reduced pressure to a preset volume, adding ethanol while stirring to generate a mixed solution, standing the mixed solution, centrifuging, and collecting precipitates in the mixed solution through alcohol precipitation; and
s500, after removing residual ethanol in the precipitate, re-dissolving the precipitate after removing the ethanol with distilled water, and performing vacuum freeze drying to obtain the cyperus esculentus crude polysaccharide.
2. The method for extracting cyperus esculentus polysaccharide according to claim 1, wherein the particle size mesh number of the cyperus esculentus powder is 80-120 mesh.
3. The process for extracting cyperus esculentus polysaccharide according to claim 2, wherein the particle size of the cyperus esculentus powder is 100 mesh.
4. The method for extracting cyperus esculentus polysaccharide according to claim 1, 2 or 3, wherein the ultrasonic conditions are that the ultrasonic temperature is 50-80 ℃, the ultrasonic power is 200-500W, and the ultrasonic time is 5-10 min.
5. The method for extracting cyperus esculentus polysaccharide according to claim 1, 2 or 3, wherein the centrifugation conditions are 5000-.
6. The method for extracting cyperus esculentus polysaccharide according to claim 4, wherein the centrifugation conditions are 5000-.
7. The method for extracting cyperus esculentus polysaccharide according to claim 1, 2 or 3, wherein the alcohol precipitation collection comprises: adding ethanol while stirring until the volume fraction is 75% v/v, standing at 4 ℃ for more than 12h, centrifuging at the rotation speed of 2500-.
8. The method for extracting cyperus esculentus polysaccharide according to claim 4, wherein the alcohol precipitation collection comprises: adding ethanol while stirring until the volume fraction is 75% v/v, standing at 4 ℃ for more than 12h, centrifuging at the rotation speed of 2500-.
9. The method for extracting cyperus esculentus polysaccharide according to claim 1, 2 or 3, wherein in step S500, the precipitate is subjected to removal of residual ethanol to obtain a dried substance, distilled water is added into the dried substance according to the liquid-to-material ratio of 5-20mL/g to obtain a re-dissolved substance, and the re-dissolved substance is subjected to vacuum freeze drying to obtain cyperus esculentus crude polysaccharide.
10. The method for extracting cyperus esculentus polysaccharide according to claim 1, 2 or 3, wherein the cyperus esculentus powder is obtained by crushing the expanded cyperus esculentus in step S100 and passing the crushed cyperus esculentus through a 100-mesh sieve, and the cyperus esculentus powder is stored in a ventilated and dried place for use.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910375309.1A CN111909282A (en) | 2019-05-07 | 2019-05-07 | Method for extracting cyperus esculentus polysaccharide |
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| CN201910375309.1A CN111909282A (en) | 2019-05-07 | 2019-05-07 | Method for extracting cyperus esculentus polysaccharide |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113699197A (en) * | 2021-09-01 | 2021-11-26 | 吉林大学 | Process for extracting cyperus esculentus oligosaccharide by enzyme method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107141357A (en) * | 2017-05-23 | 2017-09-08 | 北京鑫科创油莎豆科技发展有限公司 | A kind of continuous method and its polyose that starch and polysaccharide are extracted from cyperue esculentus |
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- 2019-05-07 CN CN201910375309.1A patent/CN111909282A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107141357A (en) * | 2017-05-23 | 2017-09-08 | 北京鑫科创油莎豆科技发展有限公司 | A kind of continuous method and its polyose that starch and polysaccharide are extracted from cyperue esculentus |
Non-Patent Citations (1)
| Title |
|---|
| 张莉弘等: ""超声辅助提取油莎豆可溶性糖"", 《东北师大学报（自然科学版）》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113699197A (en) * | 2021-09-01 | 2021-11-26 | 吉林大学 | Process for extracting cyperus esculentus oligosaccharide by enzyme method |
| CN113699197B (en) * | 2021-09-01 | 2023-04-07 | 吉林大学 | Process for extracting cyperus esculentus oligosaccharide by enzyme method |
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