CN111903665B - Liquid storage device and isolated organ perfusion system - Google Patents
Liquid storage device and isolated organ perfusion system Download PDFInfo
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- CN111903665B CN111903665B CN202010823548.1A CN202010823548A CN111903665B CN 111903665 B CN111903665 B CN 111903665B CN 202010823548 A CN202010823548 A CN 202010823548A CN 111903665 B CN111903665 B CN 111903665B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0247—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components for perfusion, i.e. for circulating fluid through organs, blood vessels or other living parts
Abstract
The invention relates to a liquid storage device and an isolated organ perfusion system, wherein the liquid storage device comprises: liquid storage container, communicating pipe and level sensor. The liquid storage container is used for containing perfusate. The communicating pipe is positioned outside the liquid storage container, and the bottom end of the communicating pipe is communicated with the liquid storage container. The liquid level sensor is arranged on the communicating pipe and used for acquiring the liquid level height on the communicating pipe. The bottom that the perfusate in the stock solution container passes through communicating pipe enters into communicating pipe, and the liquid level height in the communicating pipe is the liquid level height of stock solution container promptly, because the bubble on the perfusate surface of stock solution container can't enter into the communicating pipe, therefore level sensor can acquire the liquid level height in the communicating pipe comparatively accurately, namely can acquire the liquid level height in the stock solution container to whether can acquire the perfusate capacity in the stock solution container comparatively accurately and predetermine the scope.
Description
Technical Field
The invention relates to the technical field of perfusion devices, in particular to a liquid storage device and an isolated organ perfusion system.
Background
Mechanical perfusion is a way of preserving and transferring organs, blood vessels of the organs are connected to an isolated organ perfusion system after the organs are obtained, and the isolated organ perfusion system continuously perfuses perfusion liquid to the isolated organs in the organ preservation and transfer stage and simultaneously supplies oxygen, nutrients and the like to the isolated organs.
In the conventional technology, an isolated organ perfusion system comprises a membrane lung, a liquid storage container, a waste liquid tank, a leukocyte filter and a dialyzer. The liquid storage container, the centrifugal pump, the leukocyte filter, the membrane lung and the isolated organ form a main path, and the liquid storage container, the peristaltic pump, the dialyzer and the waste liquid tank form a branch. Because the price of the perfusate of installing in the stock solution container is comparatively expensive to and the perfusate can only satisfy the nutritional requirement in isolated organ to reach sufficient volume, need control the capacity of the perfusate in the stock solution container for predetermineeing the scope (for example 1.9L to 2.1L), make the perfusate of installing in the stock solution container be unlikely to too much and lead to the cost increase, also be so little as not to satisfy the nutritional requirement in isolated organ. However, there are many bubbles on the surface of the perfusion fluid in the liquid storage container, which results in that the height of the perfusion fluid level in the liquid storage container cannot be accurately obtained, and the volume of the perfusion fluid in the liquid storage container cannot be accurately determined, and it is difficult to control the volume of the perfusion fluid in the liquid storage container within a preset range.
Disclosure of Invention
Therefore, it is necessary to overcome the defects of the prior art, and provide a liquid storage device and an isolated organ perfusion system, which can accurately obtain whether the volume of perfusion liquid in a liquid storage container is within a preset range.
The technical scheme is as follows: a fluid reservoir, the fluid reservoir comprising: the liquid storage container is used for containing perfusate; the communicating pipe is positioned outside the liquid storage container, and the bottom end of the communicating pipe is communicated with the liquid storage container; the liquid level sensor is arranged on the communicating pipe and used for acquiring the liquid level height on the communicating pipe.
Foretell stock solution device, the bottom that the perfusate in the stock solution container passes through communicating pipe enters into communicating pipe, and the liquid level height in the communicating pipe is the liquid level height of stock solution container promptly, because the bubble on the perfusate surface of stock solution container can't enter into in the communicating pipe, therefore level sensor can acquire the liquid level height in the communicating pipe comparatively accurately, namely can acquire the liquid level height in the stock solution container to whether can acquire the perfusate capacity in the stock solution container comparatively accurately and predetermine the scope.
In one embodiment, the liquid level sensor is a light sensor, the light sensor is disposed outside the communicating tube, and the communicating tube is a transparent tube or a translucent tube.
In one embodiment, the top end of the communicating pipe is communicated with the liquid storage container; or, the pipe wall of the communicating pipe is provided with scale marks.
In one embodiment, the middle part of the bottom wall of the liquid storage container is upwards bulged to form a trapezoid boss, and the area of the top surface of the trapezoid boss is smaller than that of the bottom surface of the trapezoid boss.
In one embodiment, the liquid storage device further comprises a flow guide frame, and the flow guide frame is arranged in the liquid storage container and is positioned above the liquid outlet of the liquid storage container.
In one embodiment, the middle part of the bottom wall of the liquid storage container is upwards bulged to form a trapezoid boss, the area of the top surface of the trapezoid boss is smaller than that of the bottom surface of the trapezoid boss, and the liquid outlet of the liquid storage container is located at the peripheral part of the bottom wall of the liquid storage container; the liquid storage device further comprises a flow guide frame, the flow guide frame is arranged between the side face of the trapezoid boss and the side wall of the liquid storage container, the flow guide frame is located above the liquid outlet of the liquid storage container, and a gap is formed between the flow guide frame and the side face of the trapezoid boss or between the flow guide frame and the side wall of the liquid storage container.
In one embodiment, the liquid storage device further comprises a bracket and a cover body, the bracket is arranged on the inner wall of the liquid storage container and is used for supporting and placing an isolated organ; the cover body is arranged at the opening part of the liquid storage container in an openable mode.
An ex vivo organ perfusion system comprising the reservoir device, the ex vivo organ perfusion system further comprising: the liquid inlet end of the centrifugal pump is communicated with the liquid storage container through a pipeline, the liquid outlet end of the centrifugal pump is communicated with the liquid inlet end of the membrane lung through a pipeline, the liquid outlet end of the membrane lung is used for being connected with an isolated organ through a pipeline, and the liquid storage container, the centrifugal pump, the membrane lung and the isolated organ form a perfusion circulation main path; the filter device comprises a first peristaltic pump, a hemodialyzer and a waste liquid bag, wherein a liquid inlet end of the first peristaltic pump is communicated with a liquid storage container through a pipeline, a liquid outlet end of the first peristaltic pump is communicated with a liquid inlet end of the hemodialyzer through a pipeline, a liquid outlet end of the hemodialyzer is communicated with the liquid storage container through a pipeline, a waste discharge end of the hemodialyzer is communicated with the waste liquid bag through a pipeline, and the liquid storage container, the first peristaltic pump and the hemodialyzer form a filtering circulation auxiliary path; the liquid replenishing bag is communicated with the liquid storage container through a pipeline, the first switch valve is arranged on the pipeline, connected to the liquid storage container, of the liquid replenishing bag, and the liquid level sensor is electrically connected with the controller.
Foretell isolated organ perfusion system, on the one hand, the bottom that the perfusate in the stock solution container passes through communicating pipe enters into communicating pipe, and the liquid level height of communicating pipe is the liquid level height of stock solution container promptly, because the bubble on the perfusate surface of stock solution container can't enter into the communicating pipe, therefore level sensor can acquire the liquid level height in the communicating pipe comparatively accurately, also be exactly can acquire the liquid level height in the stock solution container to whether can comparatively accurately acquire the perfusate capacity in the stock solution container at preset scope. On the other hand, the controller is according to the corresponding aperture size of controlling first ooff valve of level sensor induction stock solution container's liquid level height information to control fluid infusion bag and give stock solution container's fluid infusion speed and fluid infusion volume size, make the perfusate volume in the stock solution container maintain balance. Specifically, the first on-off valve is, for example, an electric pinch valve. The electric pinch valve is clamped on a pipeline of the fluid infusion bag connected to the fluid storage container, and the fluid infusion flow of the fluid infusion bag is controlled by controlling the opening degree. In addition, the electric pinch valve can not contact the supply liquid, so that the supply liquid can be prevented from being polluted, the supply liquid can be recycled, and disposable consumables are not needed.
In one embodiment, the isolated organ perfusion system further comprises a leukocyte filter and a micro-plug filter, wherein the leukocyte filter is arranged on the filtration circulation auxiliary circuit; the micro-suppository filter is arranged on the perfusion circulation main path or the filtration circulation auxiliary path.
In one embodiment, the isolated organ perfusion system further comprises a PH monitor, wherein the PH monitor is electrically connected with the controller and is used for acquiring the PH value of the perfusate.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention.
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a liquid storage device according to an embodiment of the invention;
FIG. 2 is a perspective view of an isolated organ perfusion system according to an embodiment of the present invention;
FIG. 3 is an enlarged schematic view of FIG. 2 at A;
FIG. 4 is a schematic sectional view of a portion of an isolated organ perfusion system according to an embodiment of the present invention;
FIG. 5 is a schematic sectional view of another part of an isolated organ perfusion system according to an embodiment of the present invention;
FIG. 6 is an enlarged schematic view of FIG. 5 at B;
FIG. 7 is a schematic view of another isolated organ perfusion system according to an embodiment of the present invention;
FIG. 8 is a simplified schematic diagram of an ex vivo organ perfusion system in accordance with an embodiment of the present invention.
10. A liquid storage device; 11. a reservoir; 111. a trapezoidal boss; 112. a liquid outlet; 113. a joint; 12. a communicating pipe; 13. a liquid level sensor; 14. a flow guiding frame; 141. a top plate; 142. a foot plate; 143. a gap; 15. a support; 16. a cover body; 20. a centrifugal pump; 30. a membrane lung; 41. a first peristaltic pump; 42. a second peristaltic pump; 50. a hemodialyzer; 61. a waste liquid bag; 71. a first on-off valve; 72. a second on-off valve; 80. a leukocyte filter; 91. a microembolus filter; 92. a first pressure sensor; 93. a fluid infusion bag; 94. a pH monitor; 95. a second pressure sensor; 96. a first temperature sensor; 97. a second temperature sensor; 98. wrapping the bag; 99. a flow sensor.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.
Referring to fig. 1 to 3, fig. 1 shows a schematic structural diagram of a liquid storage device 10 in an embodiment of the present invention, fig. 2 shows a structural diagram of an isolated organ perfusion system in an embodiment of the present invention from a perspective, and fig. 3 shows an enlarged schematic structural diagram of fig. 2 at a point a. In a liquid storage device 10 according to an embodiment of the present invention, the liquid storage device 10 includes: a liquid storage container 11, a communicating pipe 12 and a liquid level sensor 13. The reservoir 11 is used for holding perfusion fluid. The communicating pipe 12 is located outside the liquid storage container 11, and the bottom end of the communicating pipe 12 is communicated with the liquid storage container 11. The liquid level sensor 13 is arranged on the communicating pipe 12, and the liquid level sensor 13 is used for acquiring the liquid level height on the communicating pipe 12.
Foretell stock solution device 10, the bottom that communicating pipe 12 was passed through to the perfusate in the stock solution container 11 enters into communicating pipe 12, and the liquid level height in communicating pipe 12 is the liquid level height of stock solution container 11 promptly, because the bubble on the perfusate surface of stock solution container 11 can't enter into communicating pipe 12 in, therefore level sensor 13 can acquire the liquid level height in communicating pipe 12 comparatively accurately, also can acquire the liquid level height in stock solution container 11 promptly to can acquire the perfusate capacity in the stock solution container 11 comparatively accurately and whether in the scope of predetermineeing.
Referring to fig. 1, the liquid level sensor 13 is a light sensor, the light sensor is disposed outside the communicating tube 12, and the communicating tube 12 is a transparent tube or a translucent tube. So, level sensor 13 need not with communicating pipe 12 in the perfusate contact, can not lead to polluting the perfusate to level sensor 13 can reuse, need not regard as disposable consumptive material, makes device cost reduction. It is understood that the liquid level sensor 13 may also be other types of sensors, such as an ultrasonic sensor, and is not limited thereto.
Referring to fig. 1, further, the top end of the communicating tube 12 is communicated with the liquid storage container 11; alternatively, the wall of the communication pipe 12 is provided with scale marks. Thus, after the top end of the communication pipe 12 is communicated with the liquid storage container 11, the perfusion fluid in the liquid storage container 11 cannot be discharged outwards through the top end of the communication pipe 12. In addition, after the scale marks are arranged on the pipe wall of the communicating pipe 12, the liquid level height of the liquid storage container 11 can be observed through the scale marks, and the use is convenient.
Referring to fig. 4 to 6, fig. 4 is a schematic structural diagram of a part of an isolated organ perfusion system according to an embodiment of the present invention, fig. 5 is a schematic structural diagram of another part of the isolated organ perfusion system according to an embodiment of the present invention, and fig. 6 is an enlarged structural diagram of fig. 5 at B. Further, the middle part of the bottom wall of the liquid storage container 11 is protruded upwards to form a trapezoidal boss 111, and the area of the top surface of the trapezoidal boss 111 is smaller than the area of the bottom surface of the trapezoidal boss 111. So, the interval width of the space region that trapezoidal boss 111's side and the lateral wall of stock solution container 11 enclose narrows down gradually from last, can realize the in-process that the perfusate in the stock solution container 11 outwards discharged like this, the perfusate assembles in the bottom region of stock solution container 11 gradually to liquid outlet 112 through the stock solution container 11 bottom outwards discharges, and the emission effect is comparatively complete, and it is less to remain the volume on the diapire wall of stock solution container 11.
It should be noted that the bottom wall of the liquid storage container 11 is generally provided with two liquid outlets 112, for example, two liquid outlets 112 are provided, one of the liquid outlets 112 is connected to the centrifugal pump 20 in the main filling cycle path through a joint 113, for example, and when the centrifugal pump 20 is in operation, the filling liquid in the liquid storage container 11 is pumped out through one of the liquid outlets 112; the other outlet 112 is connected to the first peristaltic pump 41 of the filter circuit, for example, via a connector 113, and the first peristaltic pump 41 is operated to draw the perfusion fluid in the reservoir 11 out through the other outlet 112. The perfusion liquid is easy to generate vortex in the liquid storage container 11 in the process of being drawn out through the liquid outlet 112, so that bubbles are generated and mixed in the perfusion liquid, and adverse effects are caused to the isolated organ.
Referring to fig. 3, 5 and 6, the liquid storage device 10 further includes a flow guiding frame 14. The diversion frame 14 is disposed in the liquid storage container 11 and above the liquid outlet 112 of the liquid storage container 11. Specifically, the projection of the diversion frame 14 on the bottom wall of the liquid storage container 11 covers the liquid outlet 112. So, under the blocking effect of water conservancy diversion frame 14, the perfusate of stock solution container 11 upper portion department can not directly discharge outwards through the liquid outlet 112 of stock solution container 11 bottom department, can avoid producing the swirl in the stock solution container 11 like this, also can effectively restrain and produce the bubble in the perfusate or sneak into the air.
Referring to fig. 3 to 6, in a specific embodiment, a central portion of a bottom wall of the liquid storage container 11 is protruded upward to form a trapezoidal boss 111, a top surface area of the trapezoidal boss 111 is smaller than a bottom surface area of the trapezoidal boss 111, and the liquid outlet 112 of the liquid storage container 11 is located at an outer peripheral portion of the bottom wall of the liquid storage container 11. The reservoir 10 also includes a flow shelf 14. The flow guiding frame 14 is disposed between the side surface of the trapezoidal boss 111 and the side wall of the liquid storage container 11, the flow guiding frame 14 is located above the liquid outlet 112 of the liquid storage container 11, and a gap 143 is disposed between the flow guiding frame 14 and the side surface of the trapezoidal boss 111 or between the flow guiding frame 14 and the side wall of the liquid storage container 11. Thus, under the blocking effect of the diversion frame 14, the perfusion fluid at the upper part of the liquid storage container 11 cannot be directly discharged outwards through the liquid outlet 112 at the bottom of the liquid storage container 11, but enters the lower area of the diversion frame 14 through the gap 143 and is discharged outwards through the liquid outlet 112, so that the vortex generated in the liquid storage container 11 can be better avoided, and the generation of bubbles or the mixing of air in the perfusion fluid can be better effectively inhibited. Specifically, the baffle frame 14 includes a top plate 141 and leg plates 142 located on both sides of the top plate 141, the leg plates 142 are connected to the bottom wall of the liquid storage container 11, and the leg plates 142 on both sides support the top plate 141 so that the top plate 141 and the bottom wall of the liquid storage container 11 form a gap. The top plate 141 is located above the liquid outlet 112 of the liquid container 11, and the top plate 141 prevents the perfusion liquid at the upper portion of the liquid container 11 from directly discharging outwards through the liquid outlet 112 at the bottom of the liquid container 11, but enters the lower area of the flow guiding frame 14 through the gap 143 and discharges outwards through the liquid outlet 112.
Referring to fig. 1 or fig. 7, fig. 7 is a schematic diagram of another perspective view of an ex vivo organ perfusion system according to an embodiment of the present invention. Further, the liquid storage device 10 further includes a bracket 15 and a cover 16. The support 15 is arranged on the inner wall of the liquid storage container 11, and the support 15 is used for supporting and placing an isolated organ. The cap 16 is detachably provided to the mouth of the liquid storage container 11.
It should be noted that, in the infringement comparison, the "flow guiding frame 14" may be a part of the "liquid storage container 11", that is, the "flow guiding frame 14" and the "other part of the liquid storage container 11" are integrally manufactured; or a separate component which can be separated from the other parts of the liquid storage container 11, namely the flow guiding frame 14 can be manufactured separately and then combined with the other parts of the liquid storage container 11 into a whole.
It should be noted that, in the infringement comparison, the "bracket 15" may be a "part of the liquid storage container 11", that is, the "bracket 15" is integrally formed with "other parts of the liquid storage container 11"; or a separate member that is separable from the other parts of the reservoir 11, i.e., the "holder 15" may be manufactured separately and then integrated with the other parts of the reservoir 11.
Referring to fig. 8, fig. 8 is a simplified diagram of an ex vivo organ perfusion system according to an embodiment of the present invention. In one embodiment, an ex vivo organ perfusion system comprises the reservoir device 10 of any of the above embodiments, and further comprises: centrifugal pump 20, membrane lung 30, first peristaltic pump 41, hemodialyzer 50, waste fluid bag 61, fluid replacement bag 93, first on-off valve 71 and controller.
The liquid inlet end of the centrifugal pump 20 is communicated with the liquid storage container 11 through a pipeline, and the liquid outlet end of the centrifugal pump 20 is communicated with the liquid inlet end of the membrane lung 30 through a pipeline. The fluid outlet end of the membrane lung 30 is used to connect to an isolated organ via a tube. The liquid storage container 11, the centrifugal pump 20, the membrane lung 30 and the isolated organ form a main perfusion circulation path. The liquid inlet end of the first peristaltic pump 41 is communicated with the liquid storage container 11 through a pipeline, and the liquid outlet end of the first peristaltic pump 41 is communicated with the liquid inlet end of the hemodialyzer 50 through a pipeline. The liquid outlet end of the hemodialyzer 50 is communicated with the liquid storage container 11 through a pipeline, and the waste discharge end of the hemodialyzer 50 is communicated with the waste liquid bag 61 through a pipeline. The reservoir 11, the first peristaltic pump 41, and the hemodialyzer 50 form a secondary filtration circuit. The fluid infusion bag 93 is communicated with the liquid storage container 11 through a pipeline. The first switch valve 71 is disposed on a pipeline of the fluid infusion bag 93 connected to the fluid storage container 11, and both the first switch valve 71 and the fluid level sensor 13 are electrically connected to the controller.
Foretell separation organ perfusion system, on the one hand, the bottom that communicating pipe 12 was passed through to the perfusate in the stock solution container 11 enters into communicating pipe 12, the liquid level height in communicating pipe 12 is the liquid level height of stock solution container 11 promptly, because the bubble on the perfusate surface of stock solution container 11 can't enter into communicating pipe 12 in, consequently level sensor 13 can acquire the liquid level height in communicating pipe 12 comparatively accurately, also can acquire the liquid level height in stock solution container 11 promptly, thereby can acquire the perfusate capacity in the stock solution container 11 comparatively accurately and whether in the scope of predetermineeing. On the other hand, the controller correspondingly controls the opening of the first switch valve 71 according to the liquid level height information of the liquid storage container 11 sensed by the liquid level sensor 13, so as to control the liquid supplementing speed and the liquid supplementing amount of the liquid supplementing bag 93 for the liquid storage container 11, and maintain the volume of the perfusion liquid in the liquid storage container 11 in a balanced manner. Specifically, the first on-off valve 71 is, for example, an electric pinch valve. The electric pinch valve is clamped on a pipeline of the fluid infusion bag 93 connected to the liquid storage container 11, and controls the fluid infusion flow of the fluid infusion bag 93 by controlling the opening. In addition, the electric pinch valve can not contact the supply liquid, so that the supply liquid can be prevented from being polluted, the supply liquid can be recycled, and disposable consumables are not needed.
Generally, the leukocyte filter 80 is connected between the membrane lung 30 and the artery by a tube. The perfusate flows out of the membrane lung 30 and then enters the leukocyte filter 80 for leukocyte filtration, so that the damage of the isolated organ caused by perfusing the perfusate is reduced, and the occurrence of organ transplant rejection reaction is effectively delayed. However, the leukocyte filter 80 is prone to blockage after long-term use (e.g. 10 hours), which may cause the perfusate in the reservoir 11 to enter the isolated organ through the main path or the main path with a small flow rate, and thus may cause the isolated organ to stop supplying oxygen and nutrients during the isolated maintenance.
Further, the ex vivo organ perfusion system further comprises a leukocyte filter 80. The leukocyte filter 80 is disposed on the filter circulation path.
The perfusate is, for example, a mixed solution of low-molecular dextran-40, sodium chloride, potassium chloride, calcium chloride, glucose, etc., and the specific components of the perfusate are not limited herein.
In addition, when the perfusate circularly flows in the perfusion circulation main path, the perfusate can be continuously perfused to the isolated organ, so that the isolated organ can be preserved for a long time in a normal temperature environment, and the problems of tissue cell damage, organ ischemia and the like caused by a low-temperature environment are effectively avoided; meanwhile, the perfusion fluid is driven to circularly flow by the power pump, so that thrombus, inflammatory factors or other foreign matter harmful substances in the isolated organ can be effectively removed, and functional vessels of the isolated organ can be maintained to be smooth, pulmonary edema can be improved, oxygenation capacity of the heart and lung can be improved, the isolated organ can be subjected to repair treatment, the risk of postoperative graft failure is reduced, and the utilization rate of the isolated organ and the success rate of transplantation operation can be effectively improved.
In addition, the fluid of the fluid infusion bag 93 is used for supplementing trace substances, such as sodium element, potassium element, etc., which are needed and consumed by the isolated organ, and is not limited herein.
The isolated organ perfusion system can realize that perfusion fluid in the liquid storage container 11 enters the isolated organ through the membrane lung 30 and circularly flows back to the liquid storage container 11 from the isolated organ under the power action of the centrifugal pump 20. Under the power action of the first peristaltic pump 41, the perfusate in the liquid storage container 11 can be filtered by the hemodialyzer 50, and after the perfusate is filtered by the hemodialyzer 50, impurities such as particles, heavy metals and the like can be filtered; wherein, because the filtering circulation auxiliary road is provided with the leucocyte filter 80, on one hand, the leucocyte filter 80 can filter the leucocyte in the perfusate, effectively reduce the damage of the isolated organ caused by perfusate perfusion, meanwhile, the occurrence of rejection reaction of organ transplantation can be effectively delayed, the utilization rate of donor organs and the success rate of transplantation operation are further improved, on the other hand, since the leukocyte filter 80 is disposed on the secondary path of the filtration cycle, rather than on the primary path of the perfusion cycle, thus, when the leukocyte filter 80 is clogged due to a long time operation (for example, 10 hours), the leukocyte filter 80 may cause the clogging of the filter circulation path, the main perfusion circulation path can still continue to work, the isolated organ can be continuously protected, and the leukocyte filter 80 on the filtering circulation path can be replaced in time.
Further, the ex vivo organ perfusion system further comprises a micro-plug filter 91. The micro-plug filter 91 is arranged on the perfusion circulation main path or the filtration circulation auxiliary path. Thus, the micro-suppository filter 91 is used for filtering various micro-suppositories in the perfusate, preventing the micro-blood vessels of the isolated organs from being embolized due to various micro-suppositories such as thrombus or air embolism, effectively improving the blood perfusion of the micro-blood vessels of the human body, and further improving the utilization rate of the donor organs and the success rate of the transplantation operation.
Further, a micro-plug filter 91 is provided on the line between the centrifugal pump 20 and the membrane lung 30. Therefore, the perfusate flowing out of the centrifugal pump 20 enters the membrane lung 30 after being filtered by the micro-suppository filter 91, so that the phenomenon that the membrane lung 30 is blocked due to the fact that the micro-suppository enters the membrane lung 30 can be avoided.
Further, a leukocyte filter 80 is provided on the line between the first peristaltic pump 41 and the hemodialyzer 50. So, leukocyte filter 80's the position of setting is comparatively reasonable, because when leukocyte filter 80 takes place to block up, the perfusate can not reentrant hemodialyzer 50 and cause harmful effects to hemodialyzer 50, and first peristaltic pump 41 still can provide power promotion perfusate and move forward in addition. Of course, alternatively, the leukocyte filter 80 may be disposed on the line from the reservoir 11 to the first peristaltic pump 41, or on the line from the hemodialyzer 50 back to the reservoir 11.
Further, the isolated organ perfusion system further comprises a second on-off valve 72. The number of the second switch valves 72 and the number of the leukocyte filters 80 are two or more, the two or more leukocyte filters 80 are arranged in parallel on the auxiliary filtration circulation path, the two or more second switch valves 72 and the two or more leukocyte filters 80 are arranged in a one-to-one correspondence, and the second switch valves 72 are used for controlling whether the corresponding leukocyte filters 80 are connected to the auxiliary filtration circulation path. Specifically, in this embodiment, there are two leukocyte filters 80, and when one of the leukocyte-encapsulating filters is connected to the filtration cycle auxiliary, the corresponding second on-off valve 72 is in an open state, the other leukocyte filter 80 is not connected to the filtration cycle auxiliary, and the second on-off valve 72 corresponding to the other leukocyte filter 80 is in a closed state. When one of the leukocyte filters 80 is blocked, the corresponding second switch valve 72 is closed, and the corresponding second switch valve 72 of the other leukocyte filter 80 is opened, so that the other leukocyte filter 80 is connected to the filtration circulation auxiliary circuit, and the filtration circulation auxiliary circuit can still perform the function of circularly filtering the perfusion fluid.
Further, the second on-off valve 72 is an electric pinch valve or a manual pinch valve for controlling the opening degree of the branch in which the leukocyte-reduction filter 80 is located. Thus, the electric pinch valve or the manual pinch valve is clamped on the branch where the leukocyte filter 80 is located, and the flow rate of the branch where the leukocyte filter 80 is located and whether the branch where the leukocyte filter 80 is located is connected to the auxiliary filtering cycle are controlled by controlling the opening degree of the branch. In addition, the electric pinch valve or the manual pinch valve can not contact the perfusion liquid, so that the perfusion liquid can be prevented from being polluted, the circulation utilization can be realized, and disposable consumables are not required.
In one embodiment, the ex vivo organ perfusion system further comprises a first pressure sensor 92. The number of the first pressure sensors 92 is two or more, and the two or more first pressure sensors 92 are provided in series in one-to-one correspondence with the two or more leukocyte filters 80. Therefore, the first pressure sensor 92 can sense the pressure of the branch of the corresponding leukocyte filter 80, and can judge whether the corresponding leukocyte filter 80 is blocked according to the pressure, so that manual judgment is not needed, and the automation degree is greatly improved. Further, in order to better judge whether the leukocyte filter 80 is clogged, the first pressure sensor 92 is disposed at both the inlet end and the outlet end of the leukocyte filter 80.
In one embodiment, the controller is electrically connected to the first pressure sensor 92 and the second switch valve 72, respectively. In this way, when the controller determines that the leukocyte filter 80 corresponding to the first pressure sensor 92 is clogged according to the first pressure sensor 92, the controller controls the other second switching valve 72 to be opened, and opens and connects the branch where the other second switching valve 72 is located to the filtration cycle auxiliary.
In one embodiment, the isolated organ perfusion system further comprises a PH monitor 94. The PH monitor 94 is electrically connected to the controller, and the PH monitor 94 is used to obtain the PH value of the perfusate. The PH monitor 94 may be disposed on the main perfusion circulation path or the auxiliary filtration circulation path, and is not limited herein. In addition, the pH monitor 94 can monitor the pH condition of the perfusate on line in real time, manual detection is replaced, and the degree of automation is high.
Alternatively, when the pH monitor 94 detects a decrease in the pH of the perfusate below a predetermined range, a pH adjusting fluid is added to the fluid replacement bag 93 to increase the pH of the perfusate.
Referring to fig. 8, the isolated organ perfusion apparatus may further include a regulation fluid bag (not shown), in which a PH regulation fluid is filled, and the regulation fluid bag is connected to the fluid reservoir 10 through a pipeline. A third on-off valve (not shown) is arranged on a pipeline connecting the regulating fluid bag to the fluid reservoir 10, and the third on-off valve is electrically connected with the controller. When the pH monitor 94 detects that the pH of the perfusate is reduced and is smaller than the preset range, the controller correspondingly controls the third switch valve to act, and the pH regulating solution in the regulating solution bag is conveyed into the liquid storage tank 10 so as to increase the pH of the perfusate and maintain the pH of the perfusate within the preset range. On the contrary, when the PH monitor 94 detects that the PH value of the perfusate is increased and is greater than the preset range, the controller correspondingly controls the third on/off valve to close, and the fluid infusion is not performed.
In one embodiment, the membrane 30 is provided with a mixed gas port for communicating with a mixed gas source (not shown in the drawings). The perfusate combines with the gas mixture in the membrane lung 30 to form an oxygenated perfusate to ensure that the perfusate flowing into the artery has sufficient oxygen content. In addition, the membrane lung 30 is communicated with a heat exchange device through a heat exchange tube to form a circulating heat exchange loop, and the perfusate exchanges heat with a heat exchange medium of the circulating heat exchange loop in the membrane lung 30 so as to keep the temperature of the perfusate suitable for the isolated organ.
In one embodiment, the ex vivo organ perfusion system further comprises a second peristaltic pump 42. The second peristaltic pump 42 is disposed on a line between the waste end of the hemodialyzer 50 and the waste liquid bag 61. The second peristaltic pump 42 can provide enough power to drive the waste liquid in the hemodialyzer 50 to be discharged into the waste liquid bag 61, so that the problem that the blood filter is not smooth in waste liquid discharge due to insufficient pressure difference is avoided.
In one embodiment, the ex vivo organ perfusion system further comprises a second pressure sensor 95, a first temperature sensor 96, and a second temperature sensor 97. The second pressure sensor 95 and the first temperature sensor 96 are disposed on the pipeline of the membrane lung 30 connected to the isolated organ, the second pressure sensor 95 is used for acquiring the pressure at the fluid outlet end of the membrane lung 30, and the first temperature sensor 96 is used for acquiring the temperature at the fluid outlet end of the membrane lung 30.
In addition, the second temperature sensor 97 is disposed on the liquid storage container 11 and is used for acquiring the temperature of the perfusion fluid in the liquid storage container 11. In addition, the ex vivo organ perfusion system further comprises a flow sensor 99. The flow sensor 99 is disposed on the pipeline between the centrifugal pump 20 and the microembolus filter 91, and the flow sensor 99 can acquire the flow of the perfusate on the pipeline between the centrifugal pump 20 and the microembolus filter 91, and can determine whether the centrifugal pump 20, the microembolus filter 91, and the membrane lung 30 are working normally according to the flow. In addition, the flow sensor 99 can be used to control the rotation speed of the centrifugal pump 20, so as to ensure that the rotation speed of the centrifugal pump 20 is within a preset range.
Referring to fig. 2 or fig. 7, in one embodiment, the isolated organ perfusion system further includes an outer sheath 98. The liquid storage device 10 is installed in the sheath 98. In addition, membrane lung 30, hemodialyzer 50, microembolus filter 91, pH monitor 94, first pressure sensor 92 and second pressure sensor 95 all set up in overcoat package 98, so overcoat package 98 can realize that devices such as stock solution container 11, membrane lung 30, hemodialyzer 50, microembolus filter 91, pH monitor 94, first pressure sensor 92 and second pressure sensor 95 are integrated together, and it is comparatively convenient to use, and occupation space is less.
The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above examples only show some embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.
Claims (10)
1. A liquid storage device, characterized in that it comprises:
the liquid storage container is used for containing perfusate;
the communicating pipe is positioned outside the liquid storage container, the bottom end of the communicating pipe is communicated with the liquid storage container, and the top end of the communicating pipe is communicated with the liquid storage container; the communicating pipe is a transparent pipe or a semitransparent pipe;
the liquid level sensor is used for acquiring the liquid level height on the communicating pipe;
the flow guide frame comprises a top plate and foot plates positioned on two sides of the top plate, the foot plates are connected with the bottom wall of the liquid storage container, and the foot plates on the two sides support the top plate, so that an interval is formed between the top plate and the bottom wall of the liquid storage container; the top plate is positioned above a liquid outlet of the liquid storage container; the middle part of the bottom wall of the liquid storage container is upwards bulged to form a trapezoidal boss, and the area of the top surface of the trapezoidal boss is smaller than that of the bottom surface of the trapezoidal boss.
2. The liquid storage device as claimed in claim 1, wherein the liquid level sensor is a light sensor, and the light sensor is disposed outside the communication pipe.
3. The liquid storage device as claimed in claim 1, wherein the wall of the communicating tube is provided with scale marks.
4. The liquid storage device as claimed in claim 1, wherein the liquid outlet of the liquid storage container is located at a peripheral portion of a bottom wall of the liquid storage container; the projection of the guide frame on the bottom wall of the liquid storage container covers the liquid outlet.
5. The liquid storage device of claim 1, wherein the flow guide frame is disposed between a side surface of the trapezoidal boss and a side wall of the liquid storage container, and a gap is provided between the flow guide frame and the side surface of the trapezoidal boss or between the flow guide frame and the side wall of the liquid storage container.
6. The liquid storage device as claimed in any one of claims 1 to 5, further comprising a support and a cover, wherein the support is arranged on the inner wall of the liquid storage container and is used for supporting and placing an isolated organ; the cover body is arranged at the opening part of the liquid storage container in an openable mode.
7. An ex vivo organ perfusion system comprising the reservoir device of any one of claims 1 to 6, the system further comprising:
the liquid inlet end of the centrifugal pump is communicated with the liquid storage container through a pipeline, the liquid outlet end of the centrifugal pump is communicated with the liquid inlet end of the membrane lung through a pipeline, the liquid outlet end of the membrane lung is used for being connected with an isolated organ through a pipeline, and the liquid storage container, the centrifugal pump, the membrane lung and the isolated organ form a perfusion circulation main path;
the filter device comprises a first peristaltic pump, a hemodialyzer and a waste liquid bag, wherein a liquid inlet end of the first peristaltic pump is communicated with a liquid storage container through a pipeline, a liquid outlet end of the first peristaltic pump is communicated with a liquid inlet end of the hemodialyzer through a pipeline, a liquid outlet end of the hemodialyzer is communicated with the liquid storage container through a pipeline, a waste discharge end of the hemodialyzer is communicated with the waste liquid bag through a pipeline, and the liquid storage container, the first peristaltic pump and the hemodialyzer form a filtering circulation auxiliary path;
the liquid replenishing bag is communicated with the liquid storage container through a pipeline, the first switch valve is arranged on the pipeline, connected to the liquid storage container, of the liquid replenishing bag, and the liquid level sensor is electrically connected with the controller.
8. The ex vivo organ perfusion system of claim 7, further comprising a leukocyte filter disposed on the filtration cycle secondary.
9. The ex-vivo organ perfusion system according to claim 7, further comprising a micro-plug filter disposed on the primary perfusion cycle path or on the secondary filtration cycle path.
10. The isolated organ perfusion system of any one of claims 7-9, further comprising a pH monitor electrically connected to the controller, the pH monitor configured to obtain a pH value of the perfusate.
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| CN111937860B (en) * | 2020-08-17 | 2021-11-09 | 无锡市人民医院 | Isolated organ perfusion system |
| CN112354575B (en) * | 2020-11-18 | 2022-04-01 | 珠海市康利莱医疗器械有限公司 | Production device and process suitable for heparin anticoagulant tube |
| US20230092486A1 (en) * | 2021-09-23 | 2023-03-23 | Lifeline Scientific, Inc. | Low fluid level detection device |
| CN115517241A (en) * | 2022-01-25 | 2022-12-27 | 佛山瑜跃医疗器械有限公司 | Isolated organ perfusion device |
| CN115316375B (en) * | 2022-08-15 | 2023-08-18 | 四川大学华西医院 | Integrated puncture perfusion catheter for perfusion of abdominal organ transplantation donor |
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