CN111903504A - Method for breeding and identifying idioplasm of two-body addition line blue wheat and translocation substitution line blue grain wheat by utilizing blue dwarf male sterile - Google Patents
Method for breeding and identifying idioplasm of two-body addition line blue wheat and translocation substitution line blue grain wheat by utilizing blue dwarf male sterile Download PDFInfo
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Abstract
The invention belongs to the technical field of breeding and identification, and relates to a method for breeding and identifying germplasm of a binary additive line blue wheat and a translocation substitution line blue grain wheat by utilizing blue dwarf male sterile. The invention provides a method for breeding germplasm of two-body additional line blue wheat and heteroreplacement line blue wheat by directly pollinating blue dwarf male sterile plants (or by carrying out radiation treatment on the blue male sterile plants and the heteroreplacement line blue wheat), and provides a time-saving breeding alternative, and the breeding efficiency is high and the directionality is strong. The method for determining the genetic type of the obtained full-blue-grain fertile plant or the obtained stable blue wheat germ plasm is provided by carrying out indoor rooting and sprouting, root tip tissue flaking and chromosome observation under a microscope, and the identification method is simple and easy to realize.
Description
Technical Field
The invention belongs to the technical field of wheat breeding, and relates to a method for breeding and identifying blue wheat germplasm of a two-body additional line blue wheat and a translocation substitution line blue grain wheat by utilizing blue dwarf male sterile.
Background
The blue wheat contains anthocyanin with antioxidant function in aleurone layer and endosperm, and can enrich microelements such as zinc, iron, chromium, selenium and the like beneficial to human body from soil, thus having stronger health care effect. Wheat is a main grain crop for people in China, and is especially the most main staple food for people in northern areas of China, and the hidden hunger caused by unbalanced nutrients can be solved through the staple food, so that the hidden health hazards are relieved. The method is the same as the method advocated by the world health organization and the international food and agriculture organization, and the method solves the problems of deficiency and imbalance of nutrients such as trace elements, vitamins and the like through biological enhancement.
The blue dwarf male sterile line is a wheat sterile line, belongs to a dominant genic male sterile line of a monomer additional line type, and comprises blue grains (4D/4E) and dwarf stalks (Rht)10) Anther abortion (Ms)2) Three dominant characters are linked and locked at 4D#On a chromosomal monomer. In the process of blue dwarf-male-sterile backcross transfer, blue-grain fertile plants with different numbers are often separated, which causes troubles for seed production work of wheat heterosis utilization (the fertile plants need to be pulled out in the hybrid seed production of the blue dwarf-male-sterile line), and on the other hand, the blue-grain fertile plants can be used for breeding blue wheat varieties. But the research on the genetic characteristics and types of the blue wheat varieties bred by the blue dwarf male-sterile way is very lacked, and the research on the breeding method and the skill for breeding the blue wheat by utilizing the blue dwarf male-sterile way is limited, so the breeding efficiency of the blue wheat varieties and the character predictability of the bred varieties are improved,our depth of investigation is far from sufficient.
Disclosure of Invention
The invention provides a novel method for breeding and identifying idioplasm of blue wheat of a two-body additional line and blue grain wheat of a translocation substitution line by utilizing blue dwarf and abortive aiming at the problem that the genetic characteristics and types of the traditional blue wheat variety are scarce.
In order to achieve the purpose, the invention is realized by adopting the following technical scheme:
a method for selectively breeding two-body additional line blue wheat and translocation substitution line blue grain wheat germplasm includes such steps as pollinating blue dwarf male-sterile group with blue dwarf male-sterile separated blue plant, sorting blue grain seeds (the blue grain seeds account for most) on the blue dwarf male-sterile plant, planting, sorting and planting blue grain seeds, and choosing full blue grain fertile plant. If the blue dwarf male-sterile population is a stable blue dwarf male-sterile population, the full blue grain fertile plant is a stable blue wheat germplasm material (if the blue dwarf male-sterile utilized is not stable, the blue grain selection plant is required to be continuously selected and bred to be stable according to a pedigree selection procedure). The blue wheat bred by the conventional blue dwarf whose kernel has high blue grain occupation ratio (about half) belongs to 42 chromosomes of heteroreplacement blue wheat (example 1); most of the blue wheat bred by the blue dwarf-male-sterile method, which has low blue grain ratio (1/4-1/3) in the seeds, belongs to the blue wheat with 44 chromosome disomic addition lines (example 2).
Specifically, M2F1The generation adopts the mixed selection of full blue grain plant selection to build a mixed population M3F24 group groups are established by combining 4 types of plant selection in the generation, and 10 plant selection in each type is selected; m4F3And the subsequent generation carries on the selective breeding to the key plant selection according to the systematic seed selection procedure; as a breeding path backup, breeding the formed 4 mixed selection clusters according to a mixed selection program; at M3F2The generation carries out the genetic type identification and confirmation on 40 confirmed key selected plants in advance, and carries out indoor germination, root tip tissue flaking and chromosome observation under a microscope on the selected plants, so that the blue wheat with 44 chromosomesThe dimeric addition system is blue wheat; the blue wheat with 42 chromosomes is the ectopic substitution line blue wheat
Preferably, at the current generation where blue-bred plants are found, neighboring blue-bred plants are bagged in the blue dwarf-male-sterile isolation region at the same time, and pollinated with the found blue-bred plants, so that the heteroreplacement line blue wheat or the disomic addition line blue wheat can be bred one year ahead of time.
Preferably, the basic materials (blue dwarf male sterile and blue grain male parent) for breeding the germplasm of the binary episome blue wheat and the heteroreplacement blue grain wheat are carried out before sowing60COr-ray (50% -60% lethal dose) treatment to increase the chance of breaking linkage, changing linkage position and increasing character exchange probability.
Preferably, the obtained full blue grain fertile plant or the obtained stable blue wheat germ plasm is subjected to germination and rooting of blue grain seeds, root tip tissue flaking and chromosome observation under a microscope to confirm the genetic type. Blue wheat with 44 chromosomes, which is a disomic line blue wheat; the blue wheat with 42 chromosomes is a heteroreplacement blue wheat.
The method can also be applied to breeding and identifying the two-body additional line blue wheat and the heteroreplacement line blue wheat by utilizing the blue-abortive germplasm.
Compared with the prior art, the invention has the advantages and positive effects that:
1. the invention provides a method for breeding germplasm of a binary additional line blue wheat and a heteroreplacement line blue wheat, namely a method for pollinating a blue dwarf male sterile population to breed the blue wheat by using a blue-bred plant separated from a blue dwarf male sterile population, creates a new breeding thought, innovatively provides a new method for breeding the blue wheat, and provides new possibility for increasing consumption varieties of the wheat.
2. The method has the advantages that indoor rooting and germination, root tip tissue flaking and chromosome observation under a microscope are carried out on the full blue grain fertile plants obtained in the breeding process in advance, the genetic types of the full blue grain fertile plants can be confirmed as soon as possible, the method is simple, the accuracy is high, the realization is easy, and the blindness of subsequent breeding is reduced.
3. By the radiation treatment of blue dwarf male sterile and blue cultivated plants, the linkage breaking, the linkage position changing and the character exchange probability increasing are increased, certain target characters of blue wheat are improved, and the breeding period is shortened.
Detailed Description
In order that the above objects, features and advantages of the present invention may be more clearly understood, the present invention will be further described with reference to specific embodiments. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced in other ways than those specifically described herein, and thus the present invention is not limited to the specific embodiments of the present disclosure.
Examples 1
The germplasm materials utilized in this example were: blue dwarf male-sterile black horse No. 1, No. wheat No. 8Blue (B)。
The main method is described as follows: the blue wheat containing 4E/4D translocation gene is used for No. 8 wheatBlue (B)"as male parent, pollinating blue dwarf male-sterile black horse No. 1 belonging to 4E/4D monomer addition line, and using before sowing60COradiating parents by using 50-60% of lethal dose of r-ray, and searching and identifying various blue-grain wheat varieties which break linkage or are generated by recombination from the dwarf male-sterile progeny of the pollination blue, and the specific steps are as follows:
A.【M0obtaining and M1Generations
Before sowing, the blue dwarf male sterile black horse No. 1 and the male parent wheat No. 8 are respectively alignedBlue (B)To carry out60COAnd (3) performing radiation treatment on 50-60% of r-ray lethal dose to increase the chances of breaking linkage, changing linkage positions, increasing character exchange probability and separating more genetic types. Adopts a 3 m sowing belt (containing 1 m reserved field walkway), and 6 lines of warp are sowed in the middle60COR-ray processed blue dwarf male-sterile black horse No. 1M0The row spacing is 25 cm, the row length is 3 m, single-grain dibble seeding is carried out, 30 grains/m and about 500 grains of blue dwarf male-sterile population scale are carried out, and a row of male parent wheat No. 8 is respectively broadcast on two sidesBlue (B)M0The male parent adopts a 5-8 cm wide seedling belt with 100 grains/m, and a 1 m wide walkway is reserved. Before the ear-picking and the flower-raising, an isolation area (about 2.2 m wide) is built, two isolation walls are built on two sides of the walkway by bamboo piles and iron wire plastic woven bags, and the walkway is about 0.8 m so as to be convenient for observation and walking. Blue wheat wheat No. 8 on either sideBlue (B)(and the separated blue-cultivated plants) freely pollinate the dwarf male sterile blue, and the plants are stirred by small bamboo rods for auxiliary pollination in the full-bloom period. In the mature period, seeds on the blue dwarf-male-sterile single plants are mainly harvested, mixed harvesting (almost all blue grains) is carried out, a small amount of white grains and lean grains are sorted and removed, and full blue grains are reserved, (the male parent blue wheat subjected to radiation treatment is independently harvested, and subsequent breeding is carried out according to a radiation breeding program, which does not belong to the category of the patent content and is not discussed).
B.【M2F1Generations
B1) The inventor analyzes that: the generation is black horse No. 1 and wheat No. 8Blue (B)The hybridization of the two varieties is only F1Generation, character separation and recombination generation is not available yet; but as M of radiation treatment2The generation is a major generation that breaks linkage, gene recombination, and trait expression, and particularly, the 4D/4E translocation gene is genetically a generation that is genetically involved in the formation of a blue-grain translocation heterosubstitution line and a blue-grain translocation disomic addition line, although the position of the gene on the chromosome may be changed, it is also a generation that requires appropriate selection.
B2) The method mainly comprises the following steps: sowing the sorted and retained blue seeds in autumn, using a 3-row sowing building with a seed sowing plate, adopting a seed sowing plate with a sowing quantity of 4 kg, adopting a 9-row area, a row spacing of 25 cm, a cell interval of 0.5 m, a cell width of 2.5 m, a row length of 20m and a cell area of 50m2Approximately 6000 population size. And (3) performing conventional fertilizer and water management in the field, observing most fertile plants in the heading and flowering period, freely pollinating partial blue dwarf-male-sterile and blue fertile plants separated in the field, selecting single plants in a small area before maturity, and selecting more plants by adopting a mixed seed selection procedure. Selecting more than 600 individual plants, simply copying seeds, eliminating the individual plants with blue and white grains, and making the blue grains have light color, thin and shrivelled, and large sizeSingle plants with uneven seeds and the like and single plants with deviation of high yield and stalk quality. 500 individuals were kept from 600 selected plants and mixed for autumn sowing.
And harvesting blue dwarf male-sterile plant seeds, sorting and eliminating thin and shrunken grains such as white grains and the like, sowing in autumn if necessary (according to the conditions of manpower and material resources), and planting and selecting the seeds as same as the seeds in the same year.
C 【M3F2Generations
C1) The inventor analyzes that: f2The generation is the most key generation of character separation, gene recombination and breeding selection, and the filial generation processed by group mixed selection method is most suitable for M3F2The generation has relatively small workload and high seed selection effect.
C2) The method mainly comprises the following steps: sowing the sorted and retained blue seeds in autumn, using a 3-row sowing building with a seed sowing plate, adopting a seed sowing plate with 4 kg sowing quantity, adopting an 18-row region, wherein the row spacing is 20 cm, the cell width is 4.0 m, the row length is 20m, and the cell area is 80m2About 1-1.2 million population size. And (3) performing conventional fertilizer and water management in the field, selecting single plants in a plot before maturity, mainly selecting excellent full-blue-grain plants, comprehensively inspecting the high yield, disease resistance, plant type growth phase and the like of the selected plants, selecting single plants of different types (big ears and multiple ears, medium stems and medium short stems, precocity and medium precocity and the like) as much as possible, and selecting multiple plants. Selecting 800-1000 single plants from the plants, and simply copying seeds to eliminate the single plants with inconsistent grain color, the single plants with grain defects such as light blue grain color, thin and flat grains, irregular sizes and the like, and the single plants with deviation of high yield and deviation of stem quality. In the seed copying process, the seeds are divided into four categories of big ears, multiple ears, short stalks and medium-short stalks according to main characters, about 100 single plants of each category are selected and reserved to be mixed into a group, and the mixed sowing is carried out in autumn to 4 group cells. According to the first 10 individuals of each type of seed, the seeds are used as the key objects of attention, and the rows of the individual seeds are prepared and threshed according to the individual ears.
C3) As the blue grain recombination genetic type (belonging to a 4D/4E translocation replacement line or a disomic translocation addition line) is determined, the progeny thereof can not be changed, so that 40 individuals requiring a single seed ear are taken, a small amount of seeds are taken, indoor germination is carried out, the root tip is made into a sheet, the chromosome number is detected, and as a result, 39 individuals belong to the disomic addition line, and 1 individual belongs to the replacement line (detected in the big ear type).
D 【M4F3And subsequent generations ]
D1)M4F3For the generation, 40 single plants selected and reserved from 4 types are sown for each single plant, 5-8 ear rows are sown for each single plant, the row length is 2 meters, the row spacing is 25 centimeters, 10 single plants of the same type are adjacently planted, the quality of each plant line is comprehensively evaluated before wheat harvest, a key plant line (two plant lines are determined according to the type of big ear, a translocation substitution line is reserved, a two-body additional line is selected and reserved) is determined from each type, the ear rows of the single plant are selected again, and the rest ear lines are replaced into the group mixed group again. And (4) breeding the key strain subsequent generations according to a systematic seed selection procedure until the key strain is stable, and comparing the yield to breed the blue-grain wheat variety.
D2) The mixed 4 kinds of mixed group colony is planted according to the group, each thin sowing of a sowing building is used as a cell, ears are simply selected from the group or selected plants are mixed to form seeds to be sown in the next generation, four or five generations are continuously sown, after stabilization, individual plants are selected from each group, all selected individual plants are uniformly identified in the field and compared with the yield, and the required blue wheat variety is selected.
E [ Breeding results ]
Finally, through the procedures of mixed selection, group selection and system selection, two blue wheat varieties Am61 and Am62 are bred in the determined key strain.
E1) Blue wheat Am61, 4D/4E translocation replacement line blue wheat grown from large ear type population. The variety belongs to a large spike type medium stem and semi-winter blue wheat variety. Rectangular ears, long awns, white glumes and blue grains, the plant height is about 78 cm, the number of ears per mu is about 33 ten thousand, the number of ears is about 45, and the weight of thousand grains is about 45 g.
E2) Blue wheat Am62, a 4D/4E disomic addition line blue wheat variety grown from a multi-panicle type strain. The variety belongs to a multi-spike type winter-biased blue wheat variety. The Chinese cabbage is characterized by being of a spindle type close to a rectangular spike, long awns, white glumes and blue grains, the plant height is about 74 cm, the number of spikes per mu is about 42 ten thousand, the number of the spikes is about 35, and the weight of each thousand grains is about 42 g.
EXAMPLES example 2
From the implementation example 1, through the steps A and B, by utilizing the blue dwarf and abortive obtained by the step B, selecting any common white-grain wheat variety as a backcross male parent, selecting blue grains (blue dwarf and abortive) for continuous backcross, identifying the sorted blue grain proportion of each line and the field blue-cultivated plant proportion of each backcross generation, presuming the genetic type of the bred blue dwarf and abortive according to the two proportions, breeding two monomer ectopic addition line type blue dwarf and abortive with relatively low proportions, and breeding a translocation substitution line blue dwarf and abortive with both higher proportions; and secondly, selecting and breeding disomic addition type blue grain wheat from the monomer ectopic addition line type blue dwarf and abortive, and obtaining the heterosubstitution line type blue grain wheat from the heterosubstitution line blue dwarf and abortive. The method comprises the following specific steps:
【M3F2generation to find the marked sterile plant and obtain blue dwarf-male-sterile again.
Blue dwarf-male-sterile seeds (the seeds are seeds which are subjected to radiation treatment by parents and are endowed with blue wheat pollen) obtained in the step B2 in the example 1 are sowed in a sowing area of 1.5 m for 6 rows, and are sowed in a single seed way for 20 seeds/m, and common white seeds are sowed on two sides as backcrossed male parents (in the example, black horse No. 1) according to a broad seedling band of 100 seeds/m. After node is pulled out to before heading, sterile plants are observed and confirmed as soon as possible, in-line adjacent fertile plants are pulled out, the whole plants are bagged before heading, black horse No. 1 is used for pollination in the glume opening period, the bagging is removed after field pollination is finished, the branding is marked, the plants are harvested according to single plants when the plants are mature, blue grains are sorted after harvesting and continuously planted, the ratio (%) of the blue grains to white grains is counted in the sorting process, and the numbers of each selected plant are filled in a table and registered.
【M4F3Planting the blue seeds of each bagged blue dwarf-male-sterile single plant sorted in summer according to the serial number sequence during autumn sowing, wherein the field layout of the generation is: reserving 1.5 m of field walkway and male parent row, sowing blue dwarf-abortive plants with the width of 1 m, the row direction is vertical to the walkway and the male parent row direction, sowing the row length 1 m by single seeds, sowing 4 rows by each single plant, uniformly sowing the common male parent Hema No. 1 by all selected blue dwarf-abortive single plants, arranging the male parents along the walkway direction, establishing a partition wall with the height of 1.2-1.3 m outside the male parents before heading, using bamboo piles, woven bags made of iron wires and other materials along the two sides of the walkway, removing the blue cultivated plants in the heading period, counting the percentage (%) of fertile plants, and filling in a table for registration. In the full-bloom stage, the young bamboo rod is used for stirring the ear of the male parent to carry out auxiliary pollination, after the pollination is finished, the facility of the isolation area is removed, the male parent at one end is cut off, the field observation and recording and other test activities are facilitated, when the wheat is mature, the wheat is singly harvested one by one according to the number of the seeding, singly harvested one by one and taken off, singly stored one by one according to the number, the blue grains and the white grains are sorted, the proportion (%) of the blue grains is counted, and the table. According to the proportion of the field fertile plants and the proportion of the sorted blue grains, the plants with similar proportions are classified into one class, and the plants are numbered again (but the original individual plant numbers and the related data recorded and registered are clearly recorded in the archives).
【M5F4Generation in autumn sowing, the ratio of the blue-grain fertile plants is arranged from low to high. The field layout of the generation is as follows: reserving a small walk of 1 meter, sowing the seeds in the area of 1.5 meters, sowing 6 rows of sorted blue dwarf-rancid blue grains, sowing the seeds in the row direction along the small walk in the field, and sowing 1 row of wide seedlings with white grains on two sides respectively and backcrossing the male parent black horse No. 1. The generation is an important generation for observing and confirming the recombination exchange rate of the blue dwarf-male-sterile blue fertile plants, and the population can be properly amplified according to the seed quantity. Single-seed dibbling, plant spacing 20 plants/meter, 5-row area, row spacing 20 cm, sowing blue seeds, dwarfing and aborting, then sowing 1 row of backcross male parents on both sides. Before ear-picking and pollen-scattering, a separation wall is built, an isolation area is built, after the nodes are pulled out, the plants are checked one by one to determine whether anthers exist, fertile plants are strictly pulled out, the pulled-out fertile plants are counted, and the ratio (%) of blue fertile plants is confirmed. And after all pollinations are finished, removing the isolation facilities, and cutting out a row of male parents in advance. In the mature period, the operations of singly collecting and removing, sorting blue grains, confirming the blue grain proportion (%) and the like are the same as the previous year.
【M6F5Autumn sowing completely according to the mode of last yearAnd (3) checking the reliability of the data of the last year, confirming the blue grain exchange rate according to the field blue-cultivated plant proportion and the sorting blue grain proportion (two proportions) of each bred blue dwarf male-sterile line, and confirming the genetic type of each blue dwarf male-sterile line through the root tip tabletting chromosome observation result.
The first step up to this point is the preliminary cultivation "MC2Blue dwarf male-sterile black horse No. 1 "(upper left symbol indicates blue grain character exchange rate of 2%); "MC42Blue dwarf male sterile line and blue dwarf male sterile line No. 1 (the upper left corner mark represents the blue grain character exchange rate of 42%). The second step is that:
1. utilize "MC2Blue dwarf male sterile black horse No. 1' breeding two-body additional line blue grain wheat
Sowing in autumn "MC2Blue seeds sorted by blue dwarf-male-sterile black horse No. 1' are sowed by using a 3-row sowing building with a sowing plate, adopting a 4-kilogram sowing quantity sowing plate, adopting a 9-row area, wherein the row spacing is 20 cm, the cell width is 2.0 m, the row length is 10 m, the colony scale is about 2000 more than one, black horse No. 1 male parents are sowed on two sides, and the area of the cell is 20m2Building a partition wall before heading, building an isolation area, bagging all the whole blue-cultivated plants (in order to prevent pollution to blue dwarf male-sterile groups) which appear after chain breaking, selecting 46 blue-cultivated plants in total, removing bagging and branding after pollination, pulling out all the plants during wheat harvesting, mixing and threshing, sorting blue and white grains, and counting the blue grain percentage to be 28.5%.
The blue grains sorted from the blue-raised plants are sown again in autumn, a 3-row sowing building with a seed sowing plate is used, a 4-kilogram sowing quantity seed sowing plate is adopted, a 9-row area is adopted, the row spacing is 20 cm, the cell width is 2.0 m, the row length is 10 m, the population scale of more than 2000 grains is adopted, the field is managed conventionally, about 100 full-blue grain single plants are selected and collected before maturity, indoor seed testing is carried out, misselected non-full-blue single plants and single plants with light blue, unsaturated and uneven grains are eliminated, the seeds of the selected full-blue grain single plants are mixed, namely, the near isogenic lines of the blue grains of the black horse No. 1 are obtained, indoor seed germination and sheet preparation are carried out, root tip staining is carried out, and chromosome microscopic examination is carried out to confirm that the blue wheat is the black horse No. 1 blue wheat of the additional line with 44 chromosomes.
2. Utilize "MC42Blue dwarf male-sterile black horse No. 1"Breeding 4D/4E translocation 4D#Substitutional blue wheat
Sowing in autumn "MC42Blue dwarf-male-sterile black horse No. 1 sorted blue grain seeds of the type can easily break the chain to generate more blue-cultivated plants, so the population scale is not too large, 6 rows of regions are adopted, the row spacing is 25 cm, the cell width is 1.5 m, the row length is 3 m, single grain dibbling is carried out, 30 grains/m is carried out, more than 500 grains of the population scale are adopted, black horse No. one male parent is sowed on two sides, partial blue-cultivated plant complete bagging is selected before ear-drawing, (so that blue dwarf-male-sterile groups are prevented from being polluted), other blue-cultivated plants which are not bagged are pulled out, the bagging is removed and branding is carried out after pollination is finished, all wheat is pulled out during harvesting, mixed threshing is carried out, blue and white grains are sorted, and the percentage of the blue grains is counted to be 53.
Sowing the blue grains sorted from the blue-bred plants again in autumn, using a 3-row sowing building with a seed sowing plate, adopting a 4 kg sowing quantity seed sowing plate, adopting a 9-row area, a row spacing of 20 cm, a cell width of 2.0 m, a row length of 10 m, a population scale of more than 2000 grains, carrying out conventional field management, before maturity, selecting and pulling out about 100 full blue grain single plants, carrying out indoor seed test, eliminating misselected non-full blue single plants and single plants with light blue, unsaturated and uneven grains, mixing the seeds of the selected full blue grain single plants, namely the blue grain near isogenic line of No. 1, carrying out indoor seed germination and root tip dyeing, and chromosome microscopic examination, and confirming that the blue wheat is 4D with 42 chromosomes#The chromosome translocation substitution line is black horse No. 1 blue wheat.
[ description of the relevant sources of germplasm materials used in the examples ]
Black horse No. 1 and No. wheat No. 8 are examined and determined varieties in Shandong province.
Blue dwarf male-male, namely a winter blue dwarf male-male (which can be obtained by paid transfer) bred by taking black horse No. 1 as a backcross male parent and carrying out continuous backcross on blue dwarf male-male 97-866 and winterization of genetic background;
the blue dwarf male sterile 97-866 germplasm (published germplasm) belongs to the vernal blue dwarf male sterile, and is agreed and cooperatively utilized by Zibo citizen breeding limited company and the Yi nationality autonomous State West Chang farmhouse of Sichuan Liang to obtain germplasm (available for paid transfer).
wheat No. 8Blue (B)The blue dwarf male-sterile wheat 8 is bred by Zibo lovemin ethnic limited company from using wheat 8 as a backcross male parent in the continuous backcross of blue dwarf male-sterile 97-866 and genetic background winterization process, and then a wheat 8 blue grain near-isogenic line is bred. (Paid transfer acquisition)
“MC2Blue dwarf male-sterile black horse No. 1'MC42Blue dwarf male sterile black horse No. 1 'two blue dwarf male sterile lines, both of which are Ziboai people's breed Limited company utilizing blue dwarf male sterile black horse No. I and blue wheat wheat No. 8 (V8)Blue (B)) The blue dwarf male-sterile line with different genetic backgrounds is formed by the treatment of parental radiation and series breeding. (Paid transfer acquisition)
The above description is only a preferred embodiment of the present invention, and not intended to limit the present invention in other forms, and any person skilled in the art may apply the above modifications or changes to the equivalent embodiments with equivalent changes, without departing from the technical spirit of the present invention, and any simple modification, equivalent change and change made to the above embodiments according to the technical spirit of the present invention still belong to the protection scope of the technical spirit of the present invention.
Claims (6)
1. A method for breeding a binary addendum line blue wheat and a translocation replacement line blue grain wheat germplasm by using blue dwarf male sterile is characterized in that a blue bred plant separated from any blue dwarf male sterile is used as a male parent to pollinate a blue dwarf male sterile group.
2. The method for breeding the germplasm of blue wheat of the disomic addition line and the blue grain wheat of the translocation substitution line by using the blue dwarf male sterile as claimed in claim 1, wherein the blue dwarf male sterile and the male parent are performed before sowing60CO r-ray radiation treatment at 50-60% lethal dose.
3. The method for identifying the germplasm of blue wheat of a disomic addition line and blue-grain wheat of a translocation substitution line is characterized in that M is a new gene2F1The generation adopts the mixed selection of full blue grain plant selection to construct the mixed selectionGroup of groups, M3F2Selecting strains in generation, combining 4 groups in 4 types, and selecting 10 strains in each type; m4F3And the subsequent generation carries on the seed selection to the selected plant according to the systematic seed selection procedure; as a breeding path backup, breeding the formed 4 mixed selection clusters according to a mixed selection program; at M3F2The generation carries out the genetic type identification confirmation on 40 confirmed selected plants in advance, and carries out indoor germination, root tip tissue flaking and chromosome observation under a microscope, and the blue wheat with 44 chromosomes is the disomic addition system blue wheat; the blue wheat with 42 chromosomes is the ectopic substitution line blue wheat.
4. The method of claim 3, wherein the blue wheat germplasm of disomic addition line and translocation replacement line is selected from the blue wheat germplasm of 44 chromosome disomic addition lines and the translocation replacement line of 42 chromosome line in the way of breeding blue dwarf and abortive with different crossover rates.
5. The method for breeding the idiotypic blue wheat and the translocation replacement line blue grain wheat germplasm by using the blue dwarf-male-sterile as claimed in claim 1 or 2, wherein the blue dwarf-male-sterile germplasm is used for breeding the idiotypic blue wheat and the heteroreplacement blue wheat.
6. The method of claim 3, wherein the blue-abortive germplasm is used to identify blue wheat of the disomic addition line and blue-dwarf germplasm of the translocation substitution line.
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