CN110301351A - A method of identification self-pollinated plant outcrossing rate - Google Patents
A method of identification self-pollinated plant outcrossing rate Download PDFInfo
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- CN110301351A CN110301351A CN201910756100.XA CN201910756100A CN110301351A CN 110301351 A CN110301351 A CN 110301351A CN 201910756100 A CN201910756100 A CN 201910756100A CN 110301351 A CN110301351 A CN 110301351A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The invention discloses a kind of methods for identifying self-pollinated plant outcrossing rate, are related to crop hybrid breeding technique field.Identification method includes selecting self-pollination plant to be measured as female parent, the self-pollination plant for carrying anti-herbicide gene is selected to be hybridized as male parent, the plant quantity for counting filial generation antiweed, the outcrossing rate of the self-pollinated plant to be measured is identified according to the plant quantity of filial generation antiweed.This method application progeny test procedures can carry out the assessment of outcrossing rate character to self-pollinated plant, eliminate the intermediate link mostly for backcross transformation sterile line, reduce breeding cost.
Description
Technical field
The present invention relates to crop hybrid breeding technique fields, in particular to a kind of identification self-pollinated plant outcrossing
The method of rate.
Background technique
Outcrossing rate (Outcrossing Rate) refers to the probability hybridized between plant Different Individual.Study plant outcrossing
There are mainly two types of methods for rate: indirect method and direct method.Indirect method is called population structural approach (Population structure
Approach, PSA), it is the association by selfing coefficient (the inbreeding coefficient, Fis) and outcrossing rate of population
Relationship estimates the method (Li, 1955) of outcrossing rate.Indirect method is obtained due to carrying out outcrossing rate estimation as unit of population
As a result population outcrossing rate is represented, indirect method can not understand the outcrossing rate of each family and variation situation in group.Indirect method is mainly used for
Analyze biological blood relationship, the longer evolutionary analysis of time span.Direct method is called progeny test procedures (Progeny-array
Approach, PAA), it is the ratio of filial generation in the specific maternal plant offspring of detection to estimate the method (Jarne of maternal plant outcrossing rate
Et al., 2008).There are 5 outcrossing offsprings for example, working as in 10 offsprings of the detection from same maternal plant, then the outcrossing of maternal plant
Rate is 50%.
From the viewpoint of tradition, soybean is a kind of typical self pollination crop, and natural outcrossing is very low, in 0.03%-
Between 19% (Caviness, 1966；Carlson and Lersten, 1987；Sun et al., 1992；Ahrent and
Cavinsee, 1994；Fujita et al., 1997；Jeffery, 2003).
Hybridization acquisition is carried out by maternal (sterile line, code name: A-Lines) × male parent (restorer, code name: R-Lines)
Hybrid soybean seed of the cenospecies as market sale.Wherein the breeding of sterile line (A-Lines) also needs maternal (infertility
System, A-Lines) × male parent (keep system, B-Lines) carries out hybridization acquisition；Restorer is Fertile material, normal to breed.Soybean
A possibility that artificial hybridization is difficult and natural outcrossing is very low, wind pollination very little (Zhao Limei etc., 1999).Patent
(CN1391795A) stated in hybrid seed producing method for soybean hybrid seed using flower thrips as soybean Pollinating Insect, have no application at present
In Production of Large Fields.
In order to guarantee sterile line propagation, cross breeding seed yield, the cenospecies for needing to select not only has advantage mesh
Character (high yield, high oil, high protein etc.) is marked, while to guarantee that there is high outcrossing rate character as maternal sterile line material.Choosing
Yield potentiality or the parent for a certain excellent character are selected, needs to obtain by least five backcross transformations more than generation
Stable sterile line.And when applying the sterile line of above-mentioned backcross transformation in production, it has been found that its outcrossing characteristics is low, breeding coefficient
Small, breeding cost is high, can not continue to use, it will lead to the waste of plenty of time, energy.Therefore, Fertile material is selected in miscellaneous
The examination in advance for carrying out outcrossing rate character before combination is handed over, the process of hybrid soybean breeding will be accelerated.
The prior art calculates outcrossing rate by measurement male sterile plants outcrossing seed-setting rate mostly, and this method is by hero
Influence of the property sterile plant on physiology and metabolic deficiency, it is difficult to comprehensively reflect the outcrossing rate of normal soybean population.
In consideration of it, the present invention is specifically proposed.
Summary of the invention
The purpose of the present invention is to provide a kind of methods for identifying self-pollinated plant outcrossing rate.This method is examined using offspring
Survey method identifies the outcrossing rate of the self-pollinated plant to be measured according to the plant quantity of filial generation antiweed, can be to certainly
Flower pollinated plant carries out the assessment of outcrossing rate character, eliminates the intermediate link mostly for backcross transformation sterile line, also avoids hero
Property sterile plant cause outcrossing seed-setting rate inaccuracy to can not comprehensively reflect just due to the difference on physiology and metabolic deficiency
The outcrossing rate of normal self-pollinated plant carries out the examination in advance of outcrossing rate character, has before being selected in cross combination to Fertile material
Conducive to the process for accelerating hybrid plant breeding, breeding cost is reduced.
The present invention is implemented as follows:
A method of identification self-pollinated plant outcrossing rate, the above method include: selection self-pollination plant to be measured
As female parent, select the self-pollination plant for carrying anti-herbicide gene as male parent, in specified Pollinating Insect or sealing condition
Naturally hybridized down, count the plant quantity of filial generation antiweed, is identified according to the plant quantity of filial generation antiweed to be measured
Self-pollinated plant outcrossing rate.
It is applied in preferred embodiment in the present invention, above-mentioned anti-herbicide gene energy antiweed.When herbicide spraying,
Non- outcrossing self-pollinated plant is withered due to the metabolic disorder without anti-herbicide gene, dead, and outcrossing self-pollination
Plant due in Matrix attachment region by hybridization containing anti-herbicide gene can herbicide-resistant, normal to survive, finally statistics is normal
The outcrossing rate of self-pollination plant to be measured is calculated in the self-pollination plant of survival.
The outcrossing rate of self-pollinated plant to be measured is calculated by following formula:
Outcrossing rate=filial generation antiweed plant quantity/total plant the number of filial generation.
Method further include: the seed culture for obtaining hybridization sprays remove corresponding with anti-herbicide gene to cotyledon period
The outcrossing rate of self-pollinated plant to be measured is identified in careless agent according to the plant quantity of filial generation antiweed.
The spraying concentration of herbicide is 0.27%-0.59%；
Preferably, the spraying concentration of herbicide is 0.37%-0.59%；
It is furthermore preferred that the spraying concentration of herbicide is 0.59%.
Outcrossing self-pollinated plant can be efficiently screened out within the scope of herbicide spraying concentration provided by the invention, if
Higher than the spraying concentration, then normal resistance outcrossing self-pollinated plant also can plant leaf it is withered, influence normal growth and development,
Outcrossing self-pollinated plant can not be screened out；If being lower than the spraying concentration, non-outcrossing self-pollinated plant blade-section is withered,
Even herbicide-tolerant can be metabolized by the own physiological of plant in subsequent one section of incubation time, restore leaf color and normal
Growth and development, lead to not distinguish outcrossing self-pollinated plant.
In the present invention using in preferred embodiment, above-mentioned anti-herbicide gene is epsps, ahas, GAT, gat4621,
Sxglr-11 or combinations thereof；
Preferably, the anti-herbicide gene is epsps.
In the present invention using in preferred embodiment, the combination of above-mentioned anti-herbicide gene can be the string of epsps and GAT
Connection combination, the tandem compound of epsps and gat4621, any one in the tandem compound of epsps and sxglr-11 can also be with
It is that anti-herbicide gene epsps, GAT, gat4621, sxglr-1 are integrated in respectively on different chromosome.
In the present invention using in preferred embodiment, the self-pollination plant of above-mentioned carrying anti-herbicide gene is also possible to
Pass through the self-pollination plant of the anti-herbicide gene of chemical mutagenesis or radioinduction breeding.
In the present invention using in preferred embodiment, above-mentioned self-pollination plant to be measured is selected from soybean, rape, rice,
Sesame, peanut, mung bean, perillaseed, strawberry or tomato；
Preferably, the self-pollination plant to be measured is selected from soybean.
In the present invention using in preferred embodiment, the self-pollination plant of above-mentioned carrying anti-herbicide gene is H03-
06-4。
It is applied in preferred embodiment in the present invention, it is above-mentioned after herbicide spraying 4-7 days, plant leaf color is investigated,
Parent's filial generation antiweed plant number is counted, and calculates the outcrossing rate of self-pollination plant to be measured.If investigation statistics plant leaf
Color time after 7 days, the non-outcrossing self-pollination plant in part may restoration ecosystem metabolism, so that blade is restored color, it is unfavorable
In the examination of subsequent plant herbicide-tolerant.If the Color time of investigation statistics plant leaf, in 4 days, the non-outcrossing in part is spent certainly
Pollination plant may not yet occur physiology and check, and leaf color is also not easy to screen.
In the present invention using in preferred embodiment, above-mentioned specified Pollinating Insect is honeybee.By providing honeybee to flower certainly
Pollinated plant is pollinated, and self-pollinated plant hybrid rate is effectively improved.
It is above-mentioned when honeybee use to be pollinated as entomophila in the present invention using in preferred embodiment, 24 squares of setting
Rice puts bee 1500, and 72 square metres of setting puts bee 3000.In addition, the quantity for putting bee can be improved in the large area production of hybrid seeds.
It is applied in preferred embodiment in the present invention, it is above-mentioned that male parent and female parent are spaced plantation, male parent and maternal row spacing
For 60-65cm, male parent and maternal spacing in the rows are 10-20cm.
The invention has the following advantages:
The present invention provides a kind of method for identifying self-pollinated plant outcrossing rate, this method utilizes anti-herbicide gene energy
Antiweed.When herbicide spraying, non-outcrossing self-pollinated plant is due to the metabolic disorder without anti-herbicide gene, in turn
It is withered, it is dead, and outcrossing self-pollinated plant is due to can be resistant to weeding containing anti-herbicide gene by hybridization in Matrix attachment region
Agent, it is normal to survive, the self-pollinated plant normally to survive is finally counted, the outcrossing rate of self-pollinated plant to be measured is calculated.
This method identifies the outcrossing rate of the self-pollinated plant to be measured according to the plant quantity of filial generation antiweed, for flower certainly
The identification of pollinated plant outcrossing rate provides new approaches, eliminates the intermediate link mostly for backcross transformation sterile line, reduces the production of hybrid seeds
Cost.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair
The restriction of range for those of ordinary skill in the art without creative efforts, can also be according to this
A little attached drawings obtain other relevant attached drawings.
Fig. 1 is solarium's perspective view；
Fig. 2 is the sectional view of solarium；
Fig. 3 is the top view of solarium；
Fig. 4 is that breeding box determines cave plate plantation schematic diagram；
Fig. 5 is 2 glyphosate spraying concentration result figure of embodiment；
Fig. 6 is the soybean plant strain growing way figure of different growing stages；
Fig. 7 is the soybean plant strain glyphosate tolerant result figure of different growing stages in embodiment 3；
Fig. 8 is that progeny population cultivates qualification figure in embodiment 4；
Fig. 9 is that progeny population cultivates qualification figure in embodiment 5.
Specific embodiment
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, according to normal conditions or manufacturer builds
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
Feature and performance of the invention are described in further detail with reference to embodiments.
A method of identification soybean outcrossing rate specifically includes the step of successively carrying out as follows:
1. transmission of heterologous pollen under the conditions of honeybee pollination:
In the First Year spring, using Soybean Germplasm to be measured as female parent, (resistance glyphosate transgenosis is from selection by H03-06-4
System) it is interior at same solarium (4m × 6m × 2m or 6m × 18m × 2m) as male parent germplasm, solarium's perspective view is shown referring to Fig.1, net
The sectional view and top view of room respectively refer to shown in Fig. 2 and Fig. 3, every cave 2-3 bunch plantings, 1 plant of final singling after emergence.Field management is same
Production of Large Fields field, timely weeding deinsectization, Post flowering forbid applying pesticide before blooming, and avoid causing to endanger to Pollinating Insect honeybee
Evil.Every part of soybean germplasm to be measured plants the cave 3-20.Nylon wire, hole mesh 1.5mm, the plantation of Parent interval, row spacing are covered before blooming
60-65cm, spacing in the rows 10-20cm, florescence place honeybee beehive.In entire soybean bloom, honeybee is fed, maintains honey
Bee bee colony density.Florescence terminates, and withdraws honeybee beehive, removes nylon wire.
Solarium is used to provide the structure of a controllable bee colony and source of pollinating in the embodiment of the present invention, in other embodiments
The control in pollination source can be realized by other easy devices.
2. the detection of soybean germplasm offspring outcrossing seed to be measured:
In the First Year autumn, the soybean germplasm to be measured of germplasm in step 1 solarium is harvested into (one strain of every plant of correspondence by single plant
System).The seed of harvest is planted in breeding box according to strain, in other embodiments, referring to shown in Fig. 4, can also be used
Breeding box is determined cave plate and is planted.Label is arranged to each strain, in order to investigate.Setting planting depth is 1-4cm, and top layer covers
Lid seedling medium soil or turfy soil, sealing of soil when prevention and treatment is irrigated.It irrigates in time, through 13-20 days, when plant is in 2
When the cotyledon period of blade, glyphosate herbicidal is sprayed to these plant, notices that medical fluid imposes on plant leaf when spraying, avoids as far as possible
It is sprayed on topsoil.Using 6-7 days, investigation survival plant, survival plant was determined as the outcrossing grain of maternal plant, according to following public affairs
Formula calculates the outcrossing rate of germplasm to be measured:
In addition, in other embodiments, the seed that step 1 harvests can also be cultivated indoors, to long to 2 leaves
When the cotyledon period of piece, glyphosate herbicidal is sprayed to these plant, therefore, germplasm outcrossing rate to be measured can be realized in 1 year
Identification, greatly shortens breeding cycle.
Embodiment 1
Using JLCMS82B and JLCMS89B as two kinds of female parent soybean lines to be measured, H03-06-4 is planted in together as male parent
One solarium (4m × 6m × 2m) covers nylon wire, hole mesh 1.5mm, the plantation of Parent interval, row spacing 60cm, spacing in the rows before blooming
10cm, florescence place honeybee beehive, totally 3 solariums (A, B and C), the detected materials JLCMS82B of each solarium and
JLCMS89B respectively chooses 5 plants.Pass through offspring's glyphosate resistance detection statistics JLCMS82B and JLCMS89B soybean outcrossing rate.
The soybean outcrossing rate of JLCMS82B and JLCMS89B kind is referring to shown in table 1:
The soybean outcrossing rate of 1 JLCMS82B and JLCMS89B kind of table
As shown in Table 1, the outcrossing rate of soybean varieties JLCMS82B is apparently higher than JLCMS89B.
Embodiment 2
The present embodiment to the normal non-transgenic soybean for the cotyledon period for growing to 2 blades and GM Roundup-Ready soyabean simultaneously into
Row 4.1%, 1.36%, 0.82%, 0.59%, 0.45%, 0.37%, 0.31%, 0.27%, 0.24% and 0.21% totally 10
Effective component concentration sprays comparative test, and the result after spraying 5 days is referring to Figure 5.Each breeding box is divided into two up and down
Region, the setting of upper and lower two regions midfeather a line, upper zone kind is implanted with 5 row h03-06-4, and (GM Roundup-Ready soyabean is free
Strain), following area kind is implanted with 5 row Jis and educates 47 (non-resistance glyphosate soybean varieties), as shown in Figure 5, effective when spraying glyphosate
When concentration is 0.59%, Non-transgenic soybean can all wither in 5 days, and the plant leaf color of GM Roundup-Ready soyabean is not
Impaired, growing way is uninfluenced.When spraying glyphosate effective concentration greater than 0.59%, Non-transgenic soybean can be in 5 days all
It is withered, and the plant leaf colour changed into yellow of part GM Roundup-Ready soyabean, it grows suppressed.It is lower than when spraying glyphosate effective concentration
When 0.59%, part Non-transgenic soybean was not presented in 5 days intra vanes seriously withers, and continues to observe, within the subsequent growth phase
Restore green, for the plant leaf color of GM Roundup-Ready soyabean without significant change, growth is normal.Therefore, when glyphosate effectively sprays
When concentration is 0.59%, identification result is best.
Embodiment 3
The present embodiment carries out glyphosate tolerance test, the soybean plant strain of different growing stages to the soybean plant strain of different growing stages
Referring to shown in Fig. 6.Control group is GM Roundup-Ready soyabean H03-06-4, and experimental group is conventional Non-transgenic soybean kind (Jilin
47), the water of two processing groups, oxygen, temperature, the conditions such as fertilizer are consistent, and implantation time is identical, and spraying effective concentration is 0.59%
The glyphosate time it is identical.Group leader will be compareed respectively to VE (germination period), VC (cotyledon period), V2, V3, V5, R1, the plant of R5 phase
0.59% glyphosate is sprayed, the processing of experimental group is identical as control group.Observation is as a result, glyphosate sprays after spraying 5 days
Phase experimental group (left side) soybean varieties in VC (cotyledon period), V2 and V3 phase are all withered, and the soybean varieties of control group (right side) are not
Impacted, spraying effect is preferable.And the experimental group soybean varieties in VE phase and R5 phase spray identification result poor, test result
Referring to shown in Fig. 7.On the one hand the present invention can shorten identification by selecting 2-3 blade cotyledon period to spray period as most suitable
On the other hand period advantageously reduces the cost of herbicide spraying.
Embodiment 4
The present embodiment is practical to provide outcrossing rate statistics for 1034 plants of soybean resources, using 1034 plants of soybean resources as
Female parent, H03-06-4 are planted in same solarium as male parent.Nylon wire, hole mesh 1.5mm, Parent interval kind are covered before blooming
It plants, row spacing 60cm, spacing in the rows 10cm, places honeybee beehive in florescence.Effective concentration is used to spray for 0.59% glyphosate
Progeny population is applied, the outcrossing rate of every plant of soybean resource is measured, referring to shown in Fig. 8 and table 2.Removal is damaged to plants caused by sudden drop in temperature and is influenced, do not emerge or
Dead seedling situation detects 868 plants of soybean resources altogether.
2 868, table soybean germplasm outcrossing rate data statistics to be measured
Embodiment 5
The practical outcrossing rate identification provided for rice varieties Ji round-grained rice 88 of the present embodiment, by 10 plants of rice varieties Ji round-grained rice 88
As female parent, K12-08 is planted in same rice seedlings pond as male parent.Wherein K12-08 is the rice generated by EMS mutagenesis
Glyphosate resistance strain.The plantation of Parent interval, line-spacing 30cm, spacing in the rows 20cm are inserted into strut column before blooming, cover Buddhist nun
Imperial net, hole mesh 1.5mm net interior placement honeybee beehive.Effective concentration is used to spray what lucky round-grained rice 88 harvested for 0.27% glyphosate
Progeny population measures the outcrossing rate of every plant of lucky round-grained rice 88, referring to shown in Fig. 9 and table 3.Removal is damaged to plants caused by sudden drop in temperature and is influenced, and is not emerged or dead seedling
Situation detects 6 plants of rice altogether.
36 plants of table rice germplasm outcrossing rate data statistics to be measured
| Number | Outcrossing grain number | Total grain number | Outcrossing rate |
| 1 | 6 | 2671 | 0.00224 |
| 2 | 3 | 3652 | 0.00082 |
| 3 | 2 | 3271 | 0.00061 |
| 4 | 0 | 4026 | 0 |
| 5 | 7 | 2638 | 0.00265 |
| 6 | 5 | 3219 | 0.00155 |
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of method for identifying self-pollinated plant outcrossing rate, which is characterized in that the described method includes: selection is to be measured from flower
Plant is pollinated as maternal, select the self-pollination plant of carrying anti-herbicide gene as male parent, in specified Pollinating Insect or
Naturally hybridized under sealing condition, the plant quantity of filial generation antiweed is counted, according to the plant quantity of filial generation antiweed
Identify the outcrossing rate of the self-pollinated plant to be measured.
2. it is according to claim 1 identification self-pollinated plant outcrossing rate method, which is characterized in that it is described it is to be measured from
The outcrossing rate of flower pollinated plant is calculated by following formula:
Outcrossing rate=filial generation antiweed plant quantity/total plant the number of filial generation.
3. the method for identification self-pollinated plant outcrossing rate according to claim 1 or 2, which is characterized in that the method
Further include: the seed culture for obtaining hybridization to cotyledon period sprays herbicide corresponding with the anti-herbicide gene, according to
The plant quantity of filial generation antiweed identifies the outcrossing rate of the self-pollinated plant to be measured.
4. the method for identification self-pollinated plant outcrossing rate according to claim 3, which is characterized in that herbicide sprays
Concentration is 0.27%-0.59%.
5. the method for identification self-pollinated plant outcrossing rate according to claim 4, which is characterized in that herbicide sprays
Concentration is 0.37%-0.59%；
Preferably, the spraying concentration of herbicide is 0.59%.
6. the method for identification self-pollinated plant outcrossing rate according to claim 1 or 2, which is characterized in that described anti-to remove
Careless agent gene is epsps, ahas, GAT, gat4621, sxglr-11 or combinations thereof；
Preferably, the anti-herbicide gene is epsps.
7. the method for identification self-pollinated plant outcrossing rate according to claim 1 or 2, which is characterized in that described to be measured
Self-pollination plant be selected from soybean, rape, rice, sesame, peanut, mung bean, perillaseed, strawberry or tomato；
Preferably, the self-pollination plant to be measured is selected from soybean.
8. the method for identification self-pollinated plant outcrossing rate according to claim 7, which is characterized in that described to carry anti-remove
The self-pollination plant of careless agent gene is H03-06-4.
9. the method for identification self-pollinated plant outcrossing rate according to claim 1, which is characterized in that the specified pollination
Insect is honeybee.
10. the method for identification self-pollinated plant outcrossing rate according to claim 1, which is characterized in that by male parent and mother
The plantation of this interval, male parent and maternal row spacing are 60-65cm, and male parent and maternal spacing in the rows are 10-20cm.
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| CN201910756100.XA CN110301351A (en) | 2019-08-15 | 2019-08-15 | A method of identification self-pollinated plant outcrossing rate |
| PCT/CN2020/071224 WO2021027249A1 (en) | 2019-08-15 | 2020-01-09 | Method for identifying outcrossing rate of self-pollination plant |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1187292A (en) * | 1997-05-09 | 1998-07-15 | 严文贵 | Method for increasing heterosis of crops and plants |
| CN1253472A (en) * | 1997-04-28 | 2000-05-17 | 严文贵 | Crop heterosis and herbicide |
| CN106165645A (en) * | 2016-05-25 | 2016-11-30 | 中国农业科学院油料作物研究所 | Cross rape producing method for seed based on Herbicid resistant and application thereof |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103828709A (en) * | 2014-03-18 | 2014-06-04 | 吉林省养蜂科学研究所 | Method for pollinating plants by worker bee laying colonies |
| CN103828708A (en) * | 2014-03-18 | 2014-06-04 | 吉林省农业科学院 | Efficient soybean cytoplasmic male sterility test-crossing method and device |
| CN106538377A (en) * | 2016-11-08 | 2017-03-29 | 上海市农业科学院 | The method of sterile line purity and its application in a kind of raising ternary hybrid rice |
| CN106962176B (en) * | 2017-03-28 | 2019-04-16 | 吉林省农业科学院 | A kind of method of efficient Large scale identification soybean germplasm to insect pollinator attraction |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1253472A (en) * | 1997-04-28 | 2000-05-17 | 严文贵 | Crop heterosis and herbicide |
| CN1187292A (en) * | 1997-05-09 | 1998-07-15 | 严文贵 | Method for increasing heterosis of crops and plants |
| CN106165645A (en) * | 2016-05-25 | 2016-11-30 | 中国农业科学院油料作物研究所 | Cross rape producing method for seed based on Herbicid resistant and application thereof |
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Application publication date: 20191008 |