CN111887262A - Compound environment-friendly biological disinfectant based on protease K - Google Patents
Compound environment-friendly biological disinfectant based on protease K Download PDFInfo
- Publication number
- CN111887262A CN111887262A CN202010816810.XA CN202010816810A CN111887262A CN 111887262 A CN111887262 A CN 111887262A CN 202010816810 A CN202010816810 A CN 202010816810A CN 111887262 A CN111887262 A CN 111887262A
- Authority
- CN
- China
- Prior art keywords
- parts
- proteinase
- disinfectant
- stabilizer
- antibacterial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/14—Boron; Compounds thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Toxicology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of biological disinfection, in particular to a proteinase K-based composite environment-friendly biological disinfectant, which overcomes the defects of common antibacterial and antivirus effects, poor effect stability and low safety factor of disinfectants in the prior art, and comprises the following raw materials in parts by weight: the antibacterial agent comprises, by weight, 10-30 parts of protease, 8-15 parts of antibacterial peptide, 10-15 parts of potassium oxide, 5-10 parts of boric acid, 4-12 parts of octoxynol and 3-8 parts of a stabilizer. The reaction rate is increased by using the octoxynol, the antibacterial and antivirus efficiency of the biological disinfectant is improved, the disinfection work can be realized in a short time, and the tricalcium aluminate serving as the stabilizer is arranged, so that the stability of the operation environment of the disinfectant is ensured, the continuous and rapid antibacterial and antivirus of the disinfectant is ensured, the effect stability is high, no irritation or toxic or side effect is caused, the application range is wide, and the method can be used for disinfecting the lodging environment of medical institutions, families, hotels, especially the weak people such as the old, children and the like.
Description
Technical Field
The invention relates to the technical field of biological disinfection, in particular to a proteinase K-based composite environment-friendly biological disinfectant.
Background
The mainstream products of current disinfectants are mainly chemical disinfectants, and the advantages and disadvantages of the disinfectants mainly comprise: 1. peroxide disinfectant: the advantages are that: the sterilization capability is strong, the oxidation capability is strong, and the sterilization agent has a killing effect on bacteria, spores and moulds; the disadvantages are as follows: unstable chemical property and strong irritation. 2. Chlorine preparations disinfectant: the advantages are that: the bactericidal capacity is strong, the irritation is small, and the bactericidal effect is slow and lasting; the disadvantages are as follows: it has little effect on virus, unstable property, and its metabolite chloroform is highly carcinogenic. 3. Iodine preparation disinfectant: the advantages are that: the stability is good, the safety is high, and the sterilizing capability is strong; the disadvantages are as follows: the biodegradability is poor, and the environment can be damaged after long-term use. 4. Alkaline disinfector: the advantages are that: has strong killing power to most reproductive bacteria and viruses; the disadvantages are as follows: it has strong irritation to poultry skin and mucosa. 5. Surfactant-based disinfectants: the advantages are that: broad-spectrum bacteriostasis and sterilization, stable property, low toxicity and small irritation; the disadvantages are as follows: the antibacterial range is small, and the compatibility is much forbidden. 6. Strong oxidant disinfectant: the advantages are that: no toxicity, no harm, no residue and no drug resistance; the disadvantages are as follows: organic substances have a certain influence on the disinfectant. 7. Aldehyde disinfectant: the advantages are that: the sterilization capability is strong, the speed is high, and the sterilization agent has effects on bacteria, fungi, spores and viruses; the disadvantages are as follows: has large irritation and certain toxicity to livestock and poultry. 8. A phenolic disinfectant: the advantages are that: can inhibit and kill common bacteria; the disadvantages are as follows: low bactericidal effect, easy pollution to environment and certain poison to livestock and poultry. 9. Alcohol disinfectants: the advantages are that: can kill bacteria propagules and destroy most lipophilic viruses; the disadvantages are as follows: the bactericidal effect is not strong, and the bactericidal composition is not effective on fungi, spores and viruses.
With the increasing attention on the pollution of people to the external environment, the search for safe and environment-friendly disinfectants is an urgent need at present. With the rapid development of biotechnology, biological disinfectants represented by biological enzymes, plant extracts, antibacterial peptides, bacteriophages and the like begin to enter the research field of disinfection science, and have the advantages of less irritation, pollution and adverse reactions compared with the traditional disinfectants. The biological enzyme is derived from the enzyme activity substances in animal and plant tissue extracts or secretions thereof, microorganism autolysates and metabolites thereof, and the biological enzyme disinfectant has wide prospect in the field of daily disinfection along with the improvement of the stability and purity of biological enzyme genetic engineering products and the reduction of the production process cost. The green environment-friendly biological disinfectant can play a role in many fields, such as the fields of disinfection of drinking water, articles for daily use, textiles and the like.
Therefore, we propose a proteinase K-based composite environment-friendly biological disinfectant to solve the above problems.
Disclosure of Invention
The invention aims to solve the defects of common antibacterial and antivirus effects, poor effect stability and low safety factor of disinfectants in the prior art, and provides a proteinase K-based composite environment-friendly biological disinfectant.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a proteinase K-based composite environment-friendly biological disinfectant, which comprises the following raw materials in parts by weight: the antibacterial agent comprises, by weight, 10-30 parts of protease, 8-15 parts of antibacterial peptide, 10-15 parts of potassium oxide, 5-10 parts of boric acid, 4-12 parts of octoxynol and 3-8 parts of a stabilizer.
Preferably, the feed comprises the following raw materials in parts by weight: 10 parts of protease K, 8 parts of antibacterial peptide, 10 parts of potassium oxide, 5 parts of boric acid, 4 parts of octoxynol and 3 parts of stabilizer.
Preferably, the feed comprises the following raw materials in parts by weight: 20 parts of protease K, 12 parts of antibacterial peptide, 12 parts of potassium oxide, 8 parts of boric acid, 8 parts of octoxynol and 5 parts of stabilizer.
Preferably, the feed comprises the following raw materials in parts by weight: 30 parts of protease K, 15 parts of antibacterial peptide, 15 parts of potassium oxide, 10 parts of boric acid, 12 parts of octoxynol and 8 parts of stabilizer.
Preferably, the stabilizer is tricalcium aluminate.
Preferably, the application method of the proteinase K-based composite environment-friendly biological disinfectant comprises the steps of adding a proteinase K-containing disinfection reaction mixture into a pH value range of 4.0-12.5, and reacting at a temperature of 50-70 ℃.
Preferably, in the disinfection reaction mixture containing the proteinase K, the pH value ranges from 7.0 to 8.0, and the reaction temperature ranges from 60 ℃ to 65 ℃.
Preferably, in the case of a sterile reaction mixture containing proteinase K, the pH value is in the range of 7.5 and the reaction temperature is 65 ℃.
Compared with the prior art, the invention has the beneficial effects that:
1. in the invention, the antibacterial peptide has a broad-spectrum antibacterial effect, cell membranes of bacteria, fungi and the like are directly destroyed, the proteinase K has a broad-spectrum protein degradation effect, and the two have certain complementarity, so that the use effect of the disinfectant is improved.
2. In the invention, in order to exert the maximum activity of the proteinase K, the temperature of the disinfection solution is increased by utilizing the potassium oxide in the implementation process of the disinfection solution, and simultaneously potassium ions required by the activity of the proteinase K can be provided, thereby achieving the optimal disinfection effect.
3. According to the invention, the reaction rate is increased by using the octoxynol, the antibacterial and antivirus efficiency of the biological disinfectant is improved, the disinfection work can be realized in a short time, and the tricalcium aluminate serving as a stabilizer is arranged, so that the stability of the operation environment of the disinfectant is ensured, the continuous and rapid antibacterial and antivirus of the disinfectant is ensured, and the disinfectant has the advantages of high effect stability, no irritation, no toxic or side effect and wide application range, and can be used for disinfecting the lodging environment of weak people such as medical institutions, families, hotels, especially the old, children and the like.
Detailed Description
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present specification, including definitions, will control. When "mass, concentration, temperature, time, or other value or parameter is expressed as a range, preferred range, or as a range defined by a list of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, a range of 1 to 50 should be understood to include any number, combination of numbers, or subrange selected from 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50, and all fractional values between the above integers, e.g., 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, and 1.9. With respect to sub-ranges, specifically consider "nested sub-ranges" that extend from any endpoint within the range. For example, nested sub-ranges of exemplary ranges 1-50 may include 1-10, 1-20, 1-30, and 1-40 in one direction, or 50-40, 50-30, 50-20, and 50-10 in another direction. "
The present invention is further illustrated below with reference to specific examples in which various processes and methods not described in detail are conventional methods well known in the art. Materials, reagents, devices, instruments, apparatuses and the like used in the following examples are commercially available unless otherwise specified.
Example one
The invention provides a proteinase K-based composite environment-friendly biological disinfectant, which comprises the following raw materials in parts by weight: 10 parts of protease K, 8 parts of antibacterial peptide, 10 parts of potassium oxide, 5 parts of boric acid, 4 parts of octoxynol and 3 parts of stabilizer.
Wherein the stabilizer is tricalcium aluminate.
Example two
The invention provides a proteinase K-based composite environment-friendly biological disinfectant, which comprises the following raw materials in parts by weight: 15 parts of protease K, 10 parts of antibacterial peptide, 12 parts of potassium oxide, 7 parts of boric acid, 72 parts of octoxynol and 5 parts of stabilizer.
Wherein the stabilizer is tricalcium aluminate.
EXAMPLE III
The invention provides a proteinase K-based composite environment-friendly biological disinfectant, which comprises the following raw materials in parts by weight: 20 parts of protease K, 12 parts of antibacterial peptide, 14 parts of potassium oxide, 8 parts of boric acid, 10 parts of octoxynol and 7 parts of stabilizer.
Wherein the stabilizer is tricalcium aluminate.
Example four
The invention provides a proteinase K-based composite environment-friendly biological disinfectant, which comprises the following raw materials in parts by weight: 30 parts of protease K, 15 parts of antibacterial peptide, 15 parts of potassium oxide, 10 parts of boric acid, 12 parts of octoxynol and 8 parts of stabilizer.
Wherein the stabilizer is tricalcium aluminate.
Comparative example 1
A biological disinfectant comprises the following raw materials in parts by weight: 15 parts of antibacterial peptide, 15 parts of potassium oxide, 10 parts of boric acid, 12 parts of octoxynol and 8 parts of stabilizer.
Wherein the stabilizer is tricalcium aluminate.
Comparative example No. two
A biological disinfectant comprises the following raw materials in parts by weight: 30 parts of protease K, 15 parts of antibacterial peptide, 15 parts of potassium oxide, 10 parts of boric acid and 8 parts of stabilizer.
Wherein the stabilizer is tricalcium aluminate.
Comparative example No. three
A biological disinfectant comprises the following raw materials in parts by weight: 30 parts of protease K, 15 parts of antibacterial peptide, 15 parts of potassium oxide, 10 parts of boric acid and 12 parts of octoxynol.
Comparative example No. four
A biological disinfectant comprises the following raw materials in parts by weight: 15 parts of antibacterial peptide, 15 parts of potassium oxide and 10 parts of boric acid.
In the above examples one to four and comparative examples one to four, which were put into the same virus suspension with the same virus titer and capacity, the detection results are shown in table 1:
TABLE 1
According to the table one, it can be seen that the reaction time of the first to fourth examples is faster, the sterilization rate is higher, and the pH value of the terminal solution is more stable, and the comparison of the first to fourth comparative examples with the fourth example as a reference shows that, in the case where no proteinase K is provided, the sterilization rate is lower because only the antimicrobial peptide is reacted alone, and the complementary promoting property is not provided, and the reaction time is increased to some extent, and the pH value of the terminal solution is lower because no proteinase K is reacted.
In comparative example two, the reaction time was significantly increased without any significant effect on the bactericidal rate and the terminal solution, since no octoxynol was added; in the third comparative example, since no stabilizer was provided, the pH value of the terminal solution was significantly increased, resulting in a poor solution reaction environment and an increase in reaction time in the reverse direction; in comparative example four, proteinase K, octoxynol and tricalcium aluminate, a stabilizer, were not added, resulting in a lower sterilization rate and an excessively long reaction time.
Therefore, in conclusion, the proteinase K can obviously improve the sterilization rate, the octoxynol can improve the reaction rate of the disinfectant and shorten the time required by treatment, and the tricalcium aluminate serving as the stabilizer is used for ensuring the stable reaction environment of the solution, promoting the reaction rate and reducing the time required by reaction.
Application example one
An application method of environment-friendly biological disinfectant based on proteinase K composite is characterized in that in a disinfection reaction mixture containing proteinase K, the pH value range is 4.0, and the reaction temperature is 50 ℃.
Application example two
An application method of environment-friendly biological disinfectant based on proteinase K composite is characterized in that in a disinfection reaction mixture containing proteinase K, the pH value range is 7.0, and the reaction temperature is 60 ℃.
Application example three
An application method of environment-friendly biological disinfectant based on proteinase K composite is that in a disinfection reaction mixture containing proteinase K, the pH value range is 8.0, and the reaction temperature is 65 ℃.
Application example four
An application method of environment-friendly biological disinfectant based on proteinase K composite is characterized in that in a disinfection reaction mixture containing proteinase K, the pH value range is 12.5, and the reaction temperature is 70 ℃.
Comparative example five
A method for applying biological disinfectant in a disinfection reaction mixture containing proteinase K, wherein the pH value is 7.0, and the reaction temperature is 50 ℃.
Comparative example six
A method for applying biological disinfectant in a disinfection reaction mixture containing proteinase K, wherein the pH value is 7.0, and the reaction temperature is 65 ℃.
Comparative example seven
A method for applying biological disinfectant in a disinfection reaction mixture containing proteinase K, wherein the pH value is 7.0, and the reaction temperature is 70 ℃.
Comparative example eight
A method for applying biological disinfectant in a disinfection reaction mixture containing proteinase K, wherein the pH value is in the range of 8.0, and the reaction temperature is 50 ℃.
Comparative example No. nine
A method for applying biological disinfectant in a disinfection reaction mixture containing proteinase K, wherein the pH value is in the range of 8.0, and the reaction temperature is 65 ℃.
Comparative example ten
A method for applying biological disinfectant in a disinfection reaction mixture containing proteinase K, wherein the pH value is in the range of 8.0, and the reaction temperature is 70 ℃.
The results of examining the processing structures of the above application examples one to four and comparative examples five to ten are shown in table 2:
TABLE 2
| Enzyme activity (U/g) | Sterilizing rate (%) | |
| Application example one | 1000 | 98.1 |
| Application example two | 1190 | 99.5 |
| Application example three | 1200 | 99.8 |
| Application example four | 1000 | 98.2 |
| Comparative example five | 1100 | 98.5 |
| Comparative example six | 1200 | 99.7 |
| Comparative example seven | 1050 | 97.8 |
| Comparative example eight | 1110 | 98.7 |
| Comparative example No. nine | 1200 | 99.6 |
| Comparative example ten | 1040 | 97.7 |
According to the table 2, the enzyme activities and the sterilization rates of the application examples II and III are obviously higher, and the comparison examples five to ten are arranged on the basis of the application examples II and III, so that the enzyme activities and the sterilization rates are still high when the reaction temperature is 60-65 ℃, and the data of the enzyme activities and the sterilization rates at other temperatures are more general.
Therefore, in conclusion, in the reaction mixture, the pH value ranges from 7 to 8, the reaction temperature ranges from 60 to 65 ℃, the proteinase K can achieve higher activity, and the killing rate on pathogenic microorganisms is higher.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (8)
1. The environment-friendly biological disinfectant based on proteinase K is characterized by comprising the following raw materials in parts by weight: the antibacterial agent comprises, by weight, 10-30 parts of protease, 8-15 parts of antibacterial peptide, 10-15 parts of potassium oxide, 5-10 parts of boric acid, 4-12 parts of octoxynol and 3-8 parts of a stabilizer.
2. The proteinase K composite environment-friendly biological disinfectant as claimed in claim 1, comprises the following raw materials in parts by weight: 10 parts of protease K, 8 parts of antibacterial peptide, 10 parts of potassium oxide, 5 parts of boric acid, 4 parts of octoxynol and 3 parts of stabilizer.
3. The proteinase K composite environment-friendly biological disinfectant as claimed in claim 1, comprises the following raw materials in parts by weight: 20 parts of protease K, 12 parts of antibacterial peptide, 12 parts of potassium oxide, 8 parts of boric acid, 8 parts of octoxynol and 5 parts of stabilizer.
4. The proteinase K composite environment-friendly biological disinfectant as claimed in claim 1, comprises the following raw materials in parts by weight: 30 parts of protease K, 15 parts of antibacterial peptide, 15 parts of potassium oxide, 10 parts of boric acid, 12 parts of octoxynol and 8 parts of stabilizer.
5. The proteinase K composite environment-friendly biological disinfectant as claimed in any one of claims 1 to 4, wherein the stabilizer is tricalcium aluminate.
6. The method for applying the proteinase K-based composite environment-friendly biological disinfectant as claimed in any one of claims 1 to 5, wherein in a disinfection reaction mixture containing proteinase K, the pH value is in a range of 4.0-12.5, and the reaction temperature is in a range of 50-70 ℃.
7. The method for applying the proteinase K composite environment-friendly biological disinfectant as claimed in claim 6, wherein in the disinfection reaction mixture containing proteinase K, the pH value is in the range of 7.0-8.0, and the reaction temperature is in the range of 60-65 ℃.
8. The method for applying the proteinase K composite environment-friendly biological disinfectant as claimed in claim 6, wherein in the disinfection reaction mixture containing proteinase K, the pH value is in the range of 7.5, and the reaction temperature is 65 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010816810.XA CN111887262A (en) | 2020-08-14 | 2020-08-14 | Compound environment-friendly biological disinfectant based on protease K |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010816810.XA CN111887262A (en) | 2020-08-14 | 2020-08-14 | Compound environment-friendly biological disinfectant based on protease K |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111887262A true CN111887262A (en) | 2020-11-06 |
Family
ID=73229203
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010816810.XA Pending CN111887262A (en) | 2020-08-14 | 2020-08-14 | Compound environment-friendly biological disinfectant based on protease K |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111887262A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1970713A (en) * | 2005-11-25 | 2007-05-30 | 沈阳宏迪科技有限公司 | Cleaning agent for machinery for food industry |
| WO2008057293A2 (en) * | 2006-10-27 | 2008-05-15 | E. I. Du Pont De Nemours And Company | Methods and compositions for prion decontamination |
| CN104782661A (en) * | 2015-04-01 | 2015-07-22 | 李�杰 | Efficient biological disinfectant for swimming pool and preparation method of efficient biological disinfectant |
| CN105031609A (en) * | 2015-07-22 | 2015-11-11 | 中国药科大学 | Disinfectant with antimicrobial peptides Cbf-14, and preparation and application of disinfectant |
| CN107197877A (en) * | 2017-07-12 | 2017-09-26 | 宿迁研美生物科技有限公司 | New bio complex enzyme virus sweep agent（Disinfectant） |
-
2020
- 2020-08-14 CN CN202010816810.XA patent/CN111887262A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1970713A (en) * | 2005-11-25 | 2007-05-30 | 沈阳宏迪科技有限公司 | Cleaning agent for machinery for food industry |
| WO2008057293A2 (en) * | 2006-10-27 | 2008-05-15 | E. I. Du Pont De Nemours And Company | Methods and compositions for prion decontamination |
| CN104782661A (en) * | 2015-04-01 | 2015-07-22 | 李�杰 | Efficient biological disinfectant for swimming pool and preparation method of efficient biological disinfectant |
| CN105031609A (en) * | 2015-07-22 | 2015-11-11 | 中国药科大学 | Disinfectant with antimicrobial peptides Cbf-14, and preparation and application of disinfectant |
| CN107197877A (en) * | 2017-07-12 | 2017-09-26 | 宿迁研美生物科技有限公司 | New bio complex enzyme virus sweep agent（Disinfectant） |
Non-Patent Citations (4)
| Title |
|---|
| 吴彤 等: "蛋白酶K的研究进展", 《食品工业科技》 * |
| 曹宋宋 等: "牛溶菌肽线性衍生物Bac8c的抑菌活性及其稳定性", 《中国食品学报》 * |
| 章检明 等: "产抗菌肽乳酸菌筛选及抗菌肽的分离纯化与特性研究", 《食品安全质量检测学报》 * |
| 郑风荣 等: "星斑川鲽免疫相关组织抗菌活性的研究", 《中国渔业质量与标准》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Borkow et al. | Copper, an ancient remedy returning to fight microbial, fungal and viral infections | |
| Landa-Solis et al. | MicrocynTM: a novel super-oxidized water with neutral pH and disinfectant activity | |
| EP1214081B1 (en) | Superoxidized water based on hypochlorous acid for the treatment of wounds | |
| CN101389221B (en) | Silver/water, silver gels and silver-based compositions, method for fabricating and using the same | |
| JP6803896B2 (en) | Antimicrobial agent containing hypochlorous acid | |
| KR100597092B1 (en) | Aqueous solution of peracetic acid and method for producing the same | |
| DE202009019172U1 (en) | Stabilized composition of an antimicrobial solution of hypochlorous acid | |
| CN110917196B (en) | Chloroquine antibacterial disinfectant and application thereof | |
| CN111248226A (en) | Novel broad-spectrum disinfectant and preparation method thereof | |
| Li et al. | Stabilizing the bactericidal activity of hydrogen peroxide: a brandnew function of certain chinese herbs | |
| JPH07265398A (en) | Disinfecting/sterilizing method for article polluted by microbe,and reaction product for disinfection/sterilization | |
| EP3432713B1 (en) | Non-alpha substituted peroxy acids and uses thereof | |
| CN101461379A (en) | Composite lysozyme disinfecting liquid | |
| JP2000136140A (en) | Aqueous solution containing substance extracted from humic soil | |
| CN105836860B (en) | A kind of stable type dioxygen aqueous disinfectant and its application in drinking water disinfection | |
| CN111280189A (en) | Disinfectant for food industry and preparation method thereof | |
| EP2362732B1 (en) | A method of obtaining high-level disinfection in a washer disinfector, and a washer disinfector | |
| CN111887262A (en) | Compound environment-friendly biological disinfectant based on protease K | |
| CN101507703B (en) | High-efficiency sterilization hands washing liquid | |
| CN113694082A (en) | Hypochlorous acid disinfectant and preparation method thereof | |
| JP5314555B2 (en) | Method for producing humus extract | |
| CN111363270A (en) | Broad-spectrum antiviral bactericidal plastic and application thereof | |
| RU2423136C2 (en) | Antiviral medication of contact action based on "betadine" and endonuclease | |
| CN111328811B (en) | Low-concentration alcohol sterilization disinfectant and application thereof | |
| JP2011502143A (en) | Bactericidal composition comprising peracid and acetylsalicylic acid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Ye Jingyu Inventor after: Zhang Zizhong Inventor after: Li Yunli Inventor after: Yang Huiming Inventor before: Ye Jingyu Inventor before: Zhang Feiyue |
|
| CB03 | Change of inventor or designer information | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20201106 |
|
| RJ01 | Rejection of invention patent application after publication |