CN111887210A - Chironomus egg collecting device and method - Google Patents
Chironomus egg collecting device and method Download PDFInfo
- Publication number
- CN111887210A CN111887210A CN202010881231.3A CN202010881231A CN111887210A CN 111887210 A CN111887210 A CN 111887210A CN 202010881231 A CN202010881231 A CN 202010881231A CN 111887210 A CN111887210 A CN 111887210A
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- CN
- China
- Prior art keywords
- chironomus
- collecting device
- egg collecting
- plate substrate
- nylon mesh
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 241000256128 Chironomus <genus> Species 0.000 title claims abstract description 72
- 238000000034 method Methods 0.000 title claims abstract description 19
- 239000004677 Nylon Substances 0.000 claims abstract description 56
- 229920001778 nylon Polymers 0.000 claims abstract description 56
- 239000004744 fabric Substances 0.000 claims abstract description 40
- 239000000758 substrate Substances 0.000 claims abstract description 40
- 239000000725 suspension Substances 0.000 claims abstract description 7
- 235000013601 eggs Nutrition 0.000 claims description 78
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 60
- 229910001220 stainless steel Inorganic materials 0.000 claims description 25
- 239000010935 stainless steel Substances 0.000 claims description 25
- 239000012535 impurity Substances 0.000 claims description 5
- 102000002322 Egg Proteins Human genes 0.000 description 5
- 108010000912 Egg Proteins Proteins 0.000 description 5
- 210000004681 ovum Anatomy 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000004062 sedimentation Methods 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- MCSXGCZMEPXKIW-UHFFFAOYSA-N 3-hydroxy-4-[(4-methyl-2-nitrophenyl)diazenyl]-N-(3-nitrophenyl)naphthalene-2-carboxamide Chemical compound Cc1ccc(N=Nc2c(O)c(cc3ccccc23)C(=O)Nc2cccc(c2)[N+]([O-])=O)c(c1)[N+]([O-])=O MCSXGCZMEPXKIW-UHFFFAOYSA-N 0.000 description 1
- 241000255925 Diptera Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 150000003278 haem Chemical class 0.000 description 1
- 230000029052 metamorphosis Effects 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
The invention discloses a chironomus egg collecting device and a chironomus egg collecting method, wherein the collecting device comprises a screen plate substrate (2), nylon mesh cloth (3) is flatly laid and fixed on the screen plate substrate, and a suspension device (1) is arranged at one end of the screen plate substrate (2).
Description
Technical Field
The invention relates to an insect egg collecting device and method, in particular to a chironomus egg collecting device and method.
Background
Chironomus belongs to the family of Insecta Diptera, and has complete metamorphosis and development in the whole life history and four stages of egg-larva-pupa-imago. The stage of the larva is longest and is divided into four instars, and most of the larvae contain heme in the body, and the body color of the larvae changes from white, transparent, light red, bright red and deep red along with growth and development, so that the larvae are easy to cause sensory sensitivity and cause water quality complaints when entering a water supply system. At present, the reason that a water supply system is polluted by chironomid larvae is known, on one hand, the water supply system belongs to subtropical climate in south China, and is long in high-temperature and high-humidity seasons, so that favorable climate conditions are provided for mass propagation of chironomid larvae; on the other hand, the water supply system has more process and management problems, such as more open structures of a water treatment plant, chironomids can fly into the water supply system to lay eggs, and the chironomids larvae with low age risk penetrate through the filter tank to cause the appearance of the chironomids larvae in the water supply system.
The collection of chironomus ovum is the basis of the relevant research of guarantee chironomus, through the collection of ovum, can carry out biological research to the chironomus on the one hand and facilitate, including many-sided research such as molecular biology, species identification, species diversity, on the other hand realizes a large amount of concentrated catches of chironomus ovum in water supply system, and carry out the ovum deactivation, can effectively reduce the production of chironomus larva in the water supply system, the guarantee quality of water that supplies water, improve the benefit level of water supply enterprise, promote people's quality of life.
At present, the concentrated collection method of chironomus eggs is less, chironomus eggs are mainly captured and artificially cultured to lay eggs to obtain the chironomus eggs, the period for obtaining the eggs is longer, the requirement on culture environmental factors is higher, and the egg laying yield of the adults cannot be controlled and guaranteed. Therefore, it is necessary to provide a convenient and fast chironomus egg sampling device and method, which can promote the biological research of micro animals, and meanwhile, the device is applied to the actual water supply production to ensure the water supply safety and provide a basis and reference for improving the water purification capacity.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention aims to provide a chironomus egg collecting device and method, so that a large number of chironomus eggs can be quickly obtained, the period of artificial culture is shortened, and the risk of uncontrollable yield caused by the risk of environmental factors is avoided.
In order to achieve the above object, the present invention provides a chironomus egg collecting device, comprising: the net plate comprises a net plate substrate (2), wherein nylon net cloth (3) is laid and fixed on the net plate substrate in a smooth mode, and a hanging device is arranged at one end of the net plate substrate (2).
Preferably, the mesh substrate (2) is a stainless steel mesh substrate.
Preferably, the stainless steel mesh plate substrate is rectangular, the length of the stainless steel mesh plate substrate is not less than 1 meter, and the width of the stainless steel mesh plate substrate is not less than 0.2 meter.
Preferably, the nylon mesh cloth (3) is black nylon mesh cloth.
Preferably, the black nylon mesh cloth has 60-100 meshes.
Preferably, a plurality of buckles (4) are respectively arranged on two sides of the long edge of the stainless steel mesh plate substrate so as to lay and fix the black nylon mesh cloth on the stainless steel mesh plate substrate (2) in a smooth manner.
Preferably, the suspension device (1) is one or more suspension ropes arranged on one side of the short side of the mesh plate substrate (2) so as to vertically suspend the chironomus egg collecting device on the long side.
In order to achieve the purpose, the invention also provides a chironomus egg collecting method, which comprises the following steps:
step S1, vertically hanging the manufactured chironomus egg collecting device in water with a long edge;
step S2, when an air-water boundary line appears on the black nylon mesh surface of the chironomus egg collecting device, vertically taking out the chironomus egg collecting device from water;
and step S3, obtaining chironomus eggs in the nylon meshes or impurities on the air-water interface near the air-water interface of the black nylon mesh.
Preferably, in step S1, the exposed height of the black nylon mesh of the chironomus egg collecting device is not less than 0.2 m.
Preferably, in step S3, the black nylon mesh is taken down smoothly, the nylon mesh with a length of 8-12 cm from the air-water interface of the black nylon mesh is obtained as a sample cloth sample, and chironomus eggs are obtained on attachments on the sample cloth sample and between openings of the cloth sample.
Compared with the prior art, the chironomus egg collecting device and the chironomus egg collecting method have the advantages that the mesh plate substrate (2) is utilized, the nylon mesh cloth (3) is laid and fixed flatly on the mesh plate substrate, the hanging device (1) is arranged at one end of the mesh plate substrate (2), the chironomus egg collecting device is manufactured, the manufactured chironomus egg collecting device is vertically hung in water by the long edge, after an air-water boundary line appears on the black nylon mesh surface of the chironomus egg collecting device, the chironomus egg collecting device is vertically taken out from the water, chironomus eggs are obtained in the nylon mesh or in impurities at the air-water interface near the air-water boundary line of the black nylon mesh surface, a large number of chironomus eggs can be obtained quickly, the period of artificial culture is shortened, and the risk of uncontrollable yield caused by the risk of environmental factors is avoided.
Drawings
FIG. 1 is a schematic structural view of a chironomus egg collecting device according to the present invention;
FIG. 2 is a flow chart of the steps of a chironomus egg collection method according to the present invention;
FIG. 3 is a schematic view of a black nylon mesh with an obvious boundary line between air and water in the embodiment of the present invention;
FIG. 4 is a schematic diagram of the application of the present invention to the attachment of a cloth sample and the obtaining of Chironomus eggs at the cloth sample opening.
Detailed Description
Other advantages and capabilities of the present invention will be readily apparent to those skilled in the art from the present disclosure by describing the embodiments of the present invention with specific embodiments thereof in conjunction with the accompanying drawings. The invention is capable of other and different embodiments and its several details are capable of modification in various other respects, all without departing from the spirit and scope of the present invention.
FIG. 1 is a schematic structural view of a chironomus egg collecting device according to the present invention. As shown in figure 1, the chironomus egg collecting device is designed as an egg collecting hanging piece and comprises a screen plate substrate 2, wherein nylon mesh cloth 3 is flatly fixed on the surface of the screen plate, the screen plate is a stainless steel screen plate, the nylon mesh cloth 3 is black nylon mesh cloth, namely a stainless steel screen plate substrate, the stainless steel screen plate substrate is rectangular, the length of the stainless steel screen plate substrate is not less than 1 meter, the width of the stainless steel screen plate substrate is not less than 0.2 meter, the black nylon mesh cloth is flatly fixed on the surface of the stainless steel screen plate, and the number of the black nylon mesh is 60-100 meshes. In the specific embodiment of the invention, the edges of two sides of the long edge of the stainless steel mesh plate substrate are respectively provided with a plurality of buckles 4 so as to lay and fix the black nylon mesh cloth on the stainless steel mesh plate substrate 2 flatly, and one side of the short edge of the stainless steel mesh plate substrate 2 is provided with a suspension device for suspending the egg collecting hanging sheet.
The chironomus egg collecting device has the following specific using process: firstly, vertically hanging the egg collecting hanging piece in water by a long edge, wherein the height of the black nylon mesh 3 exposed out of the water surface is not less than 0.2 m; suspending the ovum collecting hanging sheet for a certain time (for example, 3-7 days, when the black nylon net surface has an obvious air-water boundary line), and vertically taking out the hanging sheet from the water; the black nylon mesh cloth 3 is stably taken down, the nylon mesh cloth with the length of 10cm above and below the air-water boundary is reserved as a sample cloth sample, and chironomus eggs can be obtained on attachments on the sample cloth sample and between openings of the cloth sample.
FIG. 2 is a flow chart of the steps of a chironomus egg collecting method of the present invention. As shown in FIG. 2, the method for collecting chironomus eggs of the present invention comprises the following steps:
and step S1, vertically hanging the prepared insect egg collecting hanging piece in water by a long edge, wherein the height of the black nylon net exposed out of the water surface is not less than 0.2 m.
And step S2, when the black nylon net surface has an air-water boundary, vertically taking out the insect egg collecting hanging piece from water. Generally, when the hanging time of the egg collecting hanging piece is 3-7 days and an obvious air-water boundary line appears on the black nylon net surface, the egg collecting hanging piece is vertically taken out from the water.
And step S3, obtaining chironomus eggs in the nylon mesh or impurities on the air-water interface near the air-water interface of the black nylon mesh. The black nylon mesh cloth is stably taken down, the nylon mesh cloth with the length of 8-12 cm (the preferred value is 10cm) from the upper part to the lower part of the air-water boundary line is obtained as a sample cloth sample, and chironomus eggs can be obtained on attachments on the sample cloth sample and between openings of the cloth sample.
Examples
Firstly, preparing a chironomus egg collecting device: the manufacturing method comprises the steps of utilizing a stainless steel screen plate with the length of 100cm multiplied by 20cm as a substrate, fixing two suspension ropes at the top end of the short side of the stainless steel screen plate with the length of 100cm multiplied by 20cm, flatly laying 40-mesh black nylon screen cloth on a stainless steel screen lining plate, and fixing the nylon screen cloth by using buckles at the long side of the stainless steel screen plate to finish the manufacturing of the chironomus egg collecting device.
Selecting a horizontal flow type sedimentation tank of a certain water treatment plant as a chironomid egg collection place, hanging the chironomid egg collection device by utilizing a hanging rope to be vertical to the water surface and tightly attached to the wall of the sedimentation tank, enabling a black nylon mesh to be exposed out of the water surface by 20cm, hanging for 7 days, enabling the black nylon mesh to have an obvious air-water intersection line 5 as shown in figure 3 (two hanging pieces are respectively taken down after the hanging pieces of the two sedimentation tanks are hung, and are parallelly placed on the ground for photographing, wherein a middle gap is the ground, the two air-water intersection lines are not aligned in the figure because the two sedimentation tanks have different hydraulic conditions and water levels), keeping the nylon mesh with the length of 10cm above and below the air-water intersection line as a sample cloth sample, observing under a microscope, obtaining chironomid eggs 6 from attachments and cloth sample orifices, and eluting the chironomid eggs for culture research as shown in figure.
In summary, the chironomus egg collecting device and the chironomus egg collecting method have the advantages that the mesh plate substrate (2) is utilized, the nylon mesh cloth (3) is laid and fixed flatly on the mesh plate substrate, the hanging device (1) is arranged at one end of the mesh plate substrate (2), the chironomus egg collecting device is manufactured, the manufactured chironomus egg collecting device is vertically hung in water with the long edge, and after an air-water boundary line appears on the black nylon mesh surface of the chironomus egg collecting device, the chironomus egg collecting device is vertically taken out from the water, the chironomus egg collecting device is arranged near the air-water boundary line of the black nylon mesh surface, chironomus eggs are obtained in a nylon mesh or impurities on the air-water interface.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Modifications and variations can be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the present invention. Therefore, the scope of the invention should be determined from the following claims.
Claims (10)
1. A chironomus egg collecting device comprising: the net plate comprises a net plate substrate (2), wherein nylon net cloth (3) is laid and fixed on the net plate substrate in a smooth mode, and a hanging device (1) is arranged at one end of the net plate substrate (2).
2. The chironomus egg collecting device as claimed in claim 1, wherein: the screen substrate (2) is a stainless steel screen substrate.
3. The chironomus egg collecting device as claimed in claim 2, wherein: the stainless steel screen plate substrate is rectangular, the length of the stainless steel screen plate substrate is not less than 1 meter, and the width of the stainless steel screen plate substrate is not less than 0.2 meter.
4. The chironomus egg collecting device as claimed in claim 3, wherein: the nylon mesh cloth (3) is black nylon mesh cloth.
5. The chironomus egg collecting device as claimed in claim 4, wherein: the mesh number of the black nylon mesh cloth is 60-100 meshes.
6. The chironomus egg collecting device as claimed in claim 4, wherein: and a plurality of buckles (4) are respectively arranged on two sides of the long edge of the stainless steel screen plate substrate so as to lay and fix the black nylon screen cloth on the stainless steel screen plate substrate (2) in a smooth manner.
7. The chironomus egg collecting device as claimed in claim 4, wherein: the suspension device (1) is one or more suspension ropes arranged on one side of the short side of the screen plate substrate (2) so as to vertically suspend the chironomus egg collecting device on the long side.
8. A chironomus egg collecting method comprises the following steps:
step S1, vertically hanging the manufactured chironomus egg collecting device in water with a long edge;
step S2, when an air-water boundary line appears on the black nylon mesh surface of the chironomus egg collecting device, vertically taking out the chironomus egg collecting device from water;
and step S3, obtaining chironomus eggs in the nylon meshes or impurities on the air-water interface near the air-water interface of the black nylon mesh.
9. The method for collecting chironomus eggs as claimed in claim 8, wherein: in step S1, the exposed height of the black nylon mesh of the chironomus egg collecting device is not less than 0.2 m.
10. The method for collecting chironomus eggs as claimed in claim 8, wherein: in step S3, the black nylon mesh is taken down smoothly, the nylon mesh having a length of 8-12 cm from the air-water boundary of the black nylon mesh is obtained as a sample cloth sample, and chironomus eggs are obtained on the attachments on the sample cloth sample and between the openings of the cloth sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010881231.3A CN111887210A (en) | 2020-08-27 | 2020-08-27 | Chironomus egg collecting device and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010881231.3A CN111887210A (en) | 2020-08-27 | 2020-08-27 | Chironomus egg collecting device and method |
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| Publication Number | Publication Date |
|---|---|
| CN111887210A true CN111887210A (en) | 2020-11-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010881231.3A Pending CN111887210A (en) | 2020-08-27 | 2020-08-27 | Chironomus egg collecting device and method |
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| CN (1) | CN111887210A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202218572U (en) * | 2011-07-14 | 2012-05-16 | 华中农业大学 | Full automatic midge incubation box |
| CN205409196U (en) * | 2016-03-29 | 2016-08-03 | 东北林业大学 | High -efficient midge culture apparatus |
| CN208446418U (en) * | 2018-07-05 | 2019-02-01 | 山东农业大学 | A kind of housefly breeding apparatus convenient for quickly collecting ovum grain and replacing feeding materials |
| CN210515885U (en) * | 2019-06-12 | 2020-05-12 | 上海城市水资源开发利用国家工程中心有限公司 | Water supply network experiment box device |
-
2020
- 2020-08-27 CN CN202010881231.3A patent/CN111887210A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202218572U (en) * | 2011-07-14 | 2012-05-16 | 华中农业大学 | Full automatic midge incubation box |
| CN205409196U (en) * | 2016-03-29 | 2016-08-03 | 东北林业大学 | High -efficient midge culture apparatus |
| CN208446418U (en) * | 2018-07-05 | 2019-02-01 | 山东农业大学 | A kind of housefly breeding apparatus convenient for quickly collecting ovum grain and replacing feeding materials |
| CN210515885U (en) * | 2019-06-12 | 2020-05-12 | 上海城市水资源开发利用国家工程中心有限公司 | Water supply network experiment box device |
Non-Patent Citations (1)
| Title |
|---|
| 陈丽芬等: "供水系统中的摇蚊幼虫检测技术", 《城镇供水》 * |
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Application publication date: 20201106 |
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