CN107094721B - Wild large native daphnia magna enrichment device and enrichment method - Google Patents
Wild large native daphnia magna enrichment device and enrichment method Download PDFInfo
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- CN107094721B CN107094721B CN201710556867.9A CN201710556867A CN107094721B CN 107094721 B CN107094721 B CN 107094721B CN 201710556867 A CN201710556867 A CN 201710556867A CN 107094721 B CN107094721 B CN 107094721B
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- enrichment
- floating cover
- daphnia magna
- rod
- light
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- 241001494246 Daphnia magna Species 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 9
- 238000007667 floating Methods 0.000 claims abstract description 49
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000000835 fiber Substances 0.000 claims abstract description 10
- 241000195493 Cryptophyta Species 0.000 claims abstract description 9
- 239000010871 livestock manure Substances 0.000 claims abstract description 9
- 239000002002 slurry Substances 0.000 claims abstract description 9
- 239000000203 mixture Substances 0.000 claims abstract description 7
- 239000000725 suspension Substances 0.000 claims description 9
- 238000007789 sealing Methods 0.000 claims description 2
- 239000004744 fabric Substances 0.000 abstract description 6
- 241000238578 Daphnia Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000007547 defect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 235000021404 traditional food Nutrition 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 238000009602 toxicology test Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K79/00—Methods or means of catching fish in bulk not provided for in groups A01K69/00 - A01K77/00, e.g. fish pumps; Detection of fish; Whale fishery
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cultivation Of Seaweed (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a wild daphnia magna enrichment device and an enrichment method, which comprise the following steps: the light shading floating cover comprises a light shading floating cover body and a plurality of enrichment rods arranged at the bottom of the light shading floating cover body, the enrichment rods comprise a lamp cavity at a mandrel and a fiber layer wrapping the surface of the lamp cavity, the light shading floating cover body is made of lightproof fabric, and a plurality of floating bodies are uniformly distributed on the bottom surface of the fabric. S1 unfolding the shading floating cover on the water surface, hanging the enrichment rod in the water below the shading floating cover, and simultaneously turning on the lamp in the lamp cavity to enable the light to penetrate through the gaps of the fiber layer; s2, after the enrichment rod attracts a certain amount of daphnia magna, the enrichment rod is fished up from the bottom of the shading floating cover by using the net bag with the rod body and washed in the collecting pool; s3 separating daphnia magna in the collecting pool after the mixture of the livestock manure and the algae slurry in the collecting pool is precipitated.
Description
Technical Field
The invention relates to a technology for enriching and harvesting daphnia magna, in particular to a wild primary daphnia magna enriching device and method, wherein daphnia magna is widely applied to the field of environmental protection.
Background
The daphnia magna is widely used for research on pesticide environment safety evaluation and is widely applied to pesticide toxicology tests. The laboratory has a great demand for daphnia magna. Especially, the primary daphnia magna in the nature needs a large amount of water, and a specific culture and harvesting method is needed so as to meet the large requirements of the test.
Disclosure of Invention
The invention overcomes the defects of the prior art and provides a wild daphnia primary enrichment device and an enrichment method.
In order to achieve the purpose, the invention adopts a technical scheme that: a field native daphnia magna enrichment device includes: the light shading floating cover comprises a light shading floating cover body and a plurality of enrichment rods arranged at the bottom of the light shading floating cover body, the enrichment rods comprise a lamp cavity at a mandrel and a fiber layer wrapping the surface of the lamp cavity, the light shading floating cover body is made of lightproof fabric, and a plurality of floating bodies are uniformly distributed on the bottom surface of the fabric.
In a preferred embodiment of the present invention, the light-shielding floating cover is an umbrella-shaped circular structure.
In a preferred embodiment of the invention, the light shielding floating cover is a strip-shaped structure which extends along the bank edge and has a width.
In a preferred embodiment of the present invention, the lamp chamber is a sealed structure, and a blue light lamp connected with a battery is disposed in the lamp chamber.
In a preferred embodiment of the invention, the enrichment rod is connected to the bottom surface of the shading floating cover in a hanging mode through a suspension wire.
In a preferred embodiment of the invention, the fiber layer on the enrichment rod is adhered with a mixture of animal manure and algae slurry.
In a preferred embodiment of the present invention, the enriching rod is further provided with a balancing weight, and the balancing weight can counteract the buoyancy effect of the lamp cavity, so that the overall density of the enriching rod is greater than or equal to the density of water.
The other technical scheme adopted by the invention is as follows: a collection method using a wild primary daphnia magna enrichment device comprises the following steps:
s1 unfolding the shading floating cover on the water surface, hanging the enrichment rod in the water below the shading floating cover, and simultaneously turning on the lamp in the lamp cavity to enable the light to penetrate through the gaps of the fiber layer;
s2, after the enrichment rod attracts a certain amount of daphnia magna, the enrichment rod is fished up from the bottom of the shading floating cover by using the net bag with the rod body and washed in the collecting pool;
s3 separating daphnia magna in the collecting pool after the mixture of the livestock manure and the algae slurry in the collecting pool is precipitated.
In a preferred embodiment of the invention, the shading floating cover is unfolded through the framework.
In a preferred embodiment of the invention, the daphnia magna cannot pass through the meshes of the net bag.
The invention solves the defects in the background technology, and has the following beneficial effects:
(1) through setting up the shading floating cover that floats on the surface of water, because the lightproof characteristic of shading floating cover, cover the natural light of surface of water top for can not shine the aquatic, for the waters of shading floating cover bottom provides a darker light and thinks the environment, then the blue light in the enrichment stick can just as little as possible disturbed this moment, the effect of its light attraction daphnia magna of performance.
(2) Because the lamp chamber periphery has wrapped up the fibrous layer, it can shelter from the blue light to a certain extent, therefore the shading floating cover can get rid of as much as possible natural light interference, and the lamp chamber middle blue light is reinforceed in the contrast, promotes the ability that the blue light attracts the daphnia magna.
(3) Through the suspension of the suspension wire, the enrichment rod can be suspended at a preset depth below the water surface, the personnel do not need to take care during suspension, and the situation that the personnel interfere the enrichment rod to attract the daphnia magna is avoided.
(4) Livestock manure and algae slurry are traditional food materials for daphnia magna, and are attracted by blue light and simultaneously attracted by food, so that the daphnia magna cannot easily leave the enrichment bar after being attracted to the daphnia magna on the enrichment bar.
Drawings
The invention is further explained below with reference to the figures and examples;
FIG. 1 is a schematic structural view of a first preferred embodiment of the present invention;
FIG. 2 is a schematic structural diagram of a second preferred embodiment of the present invention;
in the figure: 1. a shading floating cover; 2. hanging wires; 3. an enrichment rod; 4. a lamp chamber; 5. a fibrous layer; 6. and (3) a framework.
Detailed Description
The invention will now be described in further detail with reference to the accompanying drawings and examples, which are simplified schematic drawings and illustrate only the basic structure of the invention in a schematic manner, and thus show only the constituents relevant to the invention.
A field native daphnia magna enrichment device includes: the shading floats the cover to and set up a plurality of enrichment stick in the shading floats the cover bottom, and the enrichment stick includes the lamp chamber of dabber department and wraps up the fibrous layer on the lamp chamber surface, and the shading floats the cover and is made by lightproof surface fabric, and what the surface fabric bottom surface equipartition was provided with a plurality of is cursory.
Through setting up the shading floating cover that floats on the surface of water, because the lightproof characteristic of shading floating cover, cover the natural light of surface of water top for can not shine the aquatic, for the waters of shading floating cover bottom provides a darker light and thinks the environment, then the blue light in the enrichment stick can just as little as possible disturbed this moment, the effect of its light attraction daphnia magna of performance.
As shown in fig. 1, the floating light-shielding cover in the first preferred embodiment of the present invention is an umbrella-shaped circular structure, and the floating light-shielding cover with the structure is suitable for collecting wild daphnia magna at a small fixed point.
As shown in fig. 2, the light-shielding floating cover in the second preferred embodiment of the present invention is a strip structure extending along the bank edge and having a wide width, and the light-shielding floating cover with such a structure is suitable for collecting wild large daphnia protozoides at a large fixed point, and the large daphnia is gathered in a shallow water area at the edge of the bank, and the light-shielding floating cover is arranged along the bank, so that the large daphnia can be collected in a targeted manner.
The lamp cavity is of a sealing structure, and a blue light lamp connected with a battery is arranged in the lamp cavity. Because the lamp chamber periphery has wrapped up the fibrous layer, it can shelter from the blue light to a certain extent, therefore the shading floating cover can get rid of as much as possible natural light interference, and the lamp chamber middle blue light is reinforceed in the contrast, promotes the ability that the blue light attracts the daphnia magna.
The enrichment rod is connected to the bottom surface of the shading floating cover in a hanging mode through a suspension wire. Through the suspension of the suspension wire, the enrichment rod can be suspended at a preset depth below the water surface, the personnel do not need to take care during suspension, and the situation that the personnel interfere the enrichment rod to attract the daphnia magna is avoided.
The fiber layer on the enrichment rod is adhered with the mixture of the livestock manure and the algae slurry. Livestock manure and algae slurry are traditional food materials for daphnia magna, and are attracted by blue light and simultaneously attracted by food, so that the daphnia magna cannot easily leave the enrichment bar after being attracted to the daphnia magna on the enrichment bar.
The enrichment rod is also provided with a balancing weight which can offset the buoyancy effect of the lamp cavity, so that the overall density of the enrichment rod is more than or equal to that of water.
A collection method using a wild primary daphnia magna enrichment device comprises the following steps:
s1 unfolding the shading floating cover on the water surface, hanging the enrichment rod in the water below the shading floating cover, and simultaneously turning on the lamp in the lamp cavity to enable the light to penetrate through the gaps of the fiber layer;
s2, after the enrichment rod attracts a certain amount of daphnia magna, the enrichment rod is fished up from the bottom of the shading floating cover by using the net bag with the rod body and washed in the collecting pool;
s3 separating daphnia magna in the collecting pool after the mixture of the livestock manure and the algae slurry in the collecting pool is precipitated.
The shading floating cover is propped open through the framework, so that the interference of wind, waves and the like can be avoided, and the framework can ensure that the shading floating cover is completely unfolded.
The daphnia magna cannot penetrate through meshes of the net bag, so that the daphnia magna cannot be lost when the enrichment bar is taken out of water as far as possible.
In light of the foregoing description of the preferred embodiment of the present invention, it is to be understood that various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (4)
1. A field native daphnia magna enrichment device includes: the light-shielding floating cover comprises a light-shielding floating cover and a plurality of enrichment rods arranged at the bottom of the light-shielding floating cover, wherein each enrichment rod comprises a lamp cavity at a central spindle and a fiber layer wrapped on the surface of the lamp cavity;
the shading floating cover is a strip-shaped structure which extends along the bank edge and has a width;
the fiber layer on the enrichment rod is adhered with a mixture of livestock manure and algae slurry;
the collection method of the enrichment device comprises the following steps:
s1 unfolding the shading floating cover on the water surface, hanging the enrichment rod in the water below the shading floating cover, and simultaneously turning on the lamp in the lamp cavity to enable the light to penetrate through the gaps of the fiber layer;
s2, after the enrichment rod attracts a certain amount of daphnia magna, the enrichment rod is fished up from the bottom of the shading floating cover by using the net bag with the rod body and washed in the collecting pool;
s3 separating daphnia magna in the collecting pool after the mixture of the livestock manure and the algae slurry in the collecting pool is precipitated.
2. The device for enriching wild daphnia magna according to claim 1, characterized in that: the lamp cavity is of a sealing structure, and a blue light lamp connected with a battery is arranged in the lamp cavity.
3. The device for enriching wild daphnia magna according to claim 1, characterized in that: the enrichment rod is connected to the bottom surface of the shading floating cover in a hanging mode through a suspension wire.
4. The device for enriching wild daphnia magna according to claim 1, characterized in that: the shading floating cover is propped open through the framework.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710556867.9A CN107094721B (en) | 2017-07-10 | 2017-07-10 | Wild large native daphnia magna enrichment device and enrichment method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710556867.9A CN107094721B (en) | 2017-07-10 | 2017-07-10 | Wild large native daphnia magna enrichment device and enrichment method |
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| Publication Number | Publication Date |
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| CN107094721A CN107094721A (en) | 2017-08-29 |
| CN107094721B true CN107094721B (en) | 2021-01-26 |
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| CN201710556867.9A Active CN107094721B (en) | 2017-07-10 | 2017-07-10 | Wild large native daphnia magna enrichment device and enrichment method |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107667930A (en) * | 2017-11-03 | 2018-02-09 | 山东省农药科学研究院 | It is easy to gather the breeding spaces and acquisition method of Daphnia magna |
| CN108439601A (en) * | 2018-03-13 | 2018-08-24 | 佛山市金净创环保技术有限公司 | The method for carrying out aquatic farm water body purification using Daphnia magna |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102388824A (en) * | 2011-08-31 | 2012-03-28 | 清华大学 | Organism adhering device |
| WO2012050220A1 (en) * | 2010-10-13 | 2012-04-19 | Sekine Toshirou | Culturing method and device for photosynthetic microorganism |
| CN103598150A (en) * | 2013-11-30 | 2014-02-26 | 哈尔滨工业大学 | Rapid cultivation device and method of daphnia magna laboratory |
| CN105967269A (en) * | 2016-05-25 | 2016-09-28 | 河海大学 | Method for removing large daphnia type zooplanktons in drinking water |
| CN205962421U (en) * | 2016-08-22 | 2017-02-22 | 台州金湖机电有限公司 | Plankton collector |
| CN206196724U (en) * | 2016-11-09 | 2017-05-31 | 山东省淡水渔业研究院 | A kind of collection finishing device of cladocera biological feed |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02190130A (en) * | 1989-01-20 | 1990-07-26 | Masahiro Kobayashi | Collecting tool for minute aquatic animal |
| CN204062842U (en) * | 2014-05-28 | 2014-12-31 | 浙江省海洋水产研究所 | A kind ofly lure the fishing focused lamp cover of variable light beam size |
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2017
- 2017-07-10 CN CN201710556867.9A patent/CN107094721B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012050220A1 (en) * | 2010-10-13 | 2012-04-19 | Sekine Toshirou | Culturing method and device for photosynthetic microorganism |
| CN102388824A (en) * | 2011-08-31 | 2012-03-28 | 清华大学 | Organism adhering device |
| CN103598150A (en) * | 2013-11-30 | 2014-02-26 | 哈尔滨工业大学 | Rapid cultivation device and method of daphnia magna laboratory |
| CN105967269A (en) * | 2016-05-25 | 2016-09-28 | 河海大学 | Method for removing large daphnia type zooplanktons in drinking water |
| CN205962421U (en) * | 2016-08-22 | 2017-02-22 | 台州金湖机电有限公司 | Plankton collector |
| CN206196724U (en) * | 2016-11-09 | 2017-05-31 | 山东省淡水渔业研究院 | A kind of collection finishing device of cladocera biological feed |
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| CN107094721A (en) | 2017-08-29 |
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Effective date of registration: 20210106 Address after: 528000 8 / 101, block 5, 33 Jinyuan street, Chancheng District, Foshan City, Guangdong Province Applicant after: Guangdong Minxin Technology Development Co.,Ltd. Address before: 528200 Zhongchuang space K09, floor 1, building 1, No.28, Dongyi street, Jiansha Road, Danzao Town, Nanhai District, Foshan City, Guangdong Province Applicant before: FOSHAN SHUICHUANG KELIAN ECOLOGICAL TECHNOLOGY Co.,Ltd. |
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