CN111876419B - Small interfering nucleic acid for inhibiting novel coronavirus, composition and application - Google Patents

Abstract

The invention discloses a small interfering RNA for inhibiting novel coronavirus, a composition and application thereof. The invention claims to protect siRNA containing a sense strand and an antisense strand, wherein the siRNA is 21-si-SP-2069, part of nucleotides of the sense strand or the antisense strand are 2' -O-methyl ribonucleotides, and part of phosphate groups are phosphorothioate groups. The invention provides novel siRNA and a composition thereof, which can effectively prevent and/or treat novel coronavirus.

Description

Small interfering nucleic acid for inhibiting novel coronavirus, composition and application

The invention relates to a small interfering RNA for inhibiting a novel coronavirus, a composition and a divisional application of the small interfering RNA, wherein the application date is 3/4/2020, and the application number is 202010256865. X.

Technical Field

The invention relates to the technical field of biological medicines, in particular to a small interfering RNA for inhibiting the expression of a novel coronavirus gene and application of the small interfering RNA in preparing a pharmaceutical composition for preventing and/or treating novel coronavirus pneumonia.

Background

The novel coronavirus pneumonia (CoronaVirus disease2019, COVID-19) is an acute infectious pneumonia, and researchers found that its pathogen is caused by a novel beta coronavirus that was not previously found in humans, which was subsequently named SARS-CoV-2 by the World Health Organization (WHO). Patients with COVID-19 mostly have the initial symptoms of fever, hypodynamia and dry cough, and gradually have severe manifestations such as dyspnea and the like. The prognosis is good in most patients and acute respiratory distress syndrome and septic shock can occur in some severe cases and even death. By 1/3 of 2020, COVID-19 has infected 88000 people, causing nearly 3000 deaths. Scientists and clinicians are working rapidly to combat the respiratory disease covi-19 caused by the new coronavirus, but there is still a lack of effective antiviral drugs against SARS-CoV-2 virus, and the current treatment is mainly isolation treatment and symptomatic support treatment. Therefore, the development of siRNA and pharmaceutical products thereof effective in preventing and/or treating novel coronavirus pneumonia is an urgent problem to be solved.

Disclosure of Invention

The invention aims to provide siRNA targeting a novel coronavirus gene and application thereof in preparation of a novel coronavirus pneumonia medicament.

The novel coronavirus (SARS-CoV-2) is similar to SARS-CoV and MERS-CoV viruses, and the genome of SARS-CoV-2 is divided into two parts, i.e., a non-structural gene and a structural gene. Among them, the nonstructural gene, which occupies about two thirds of the total length of the SARS-CoV-2 genome, contains two sequences encoding proteins, designated ORF1a and ORF1 b. The structural gene region immediately after the sequence encodes structural proteins such as S protein, orf3a protein, E protein, M protein, orf6 protein, orf7a protein, orf8 protein, and N protein.

The invention provides a siRNA molecule, which contains a complementary sense strand and an antisense strand, wherein the sense strand contains a nucleotide sequence shown as SEQ ID NO: 5, and the antisense strand comprises the nucleotide sequence shown as SEQ ID NO: 6; alternatively, the sense strand comprises the sequence set forth as SEQ ID NO: 9, and the antisense strand contains a nucleotide sequence shown as SEQ ID NO: 10; alternatively, the sense strand comprises the sequence set forth as SEQ ID NO: 57, and the antisense strand comprises the nucleotide sequence shown as SEQ ID NO: 58; alternatively, the sense strand comprises the sequence set forth as SEQ ID NO: 59, and the antisense strand comprises the nucleotide sequence shown as SEQ ID NO: 60; alternatively, the sense strand comprises the sequence set forth as SEQ ID NO: 69, and the antisense strand comprises the nucleotide sequence shown as SEQ ID NO: 70; alternatively, the sense strand comprises the sequence set forth as SEQ ID NO: 123, and the antisense strand comprises the nucleotide sequence shown as SEQ ID NO: 124; alternatively, the sense strand comprises the sequence set forth as SEQ ID NO: 131, and the antisense strand comprises the nucleotide sequence shown as SEQ ID NO: 132; alternatively, the sense strand comprises the sequence set forth as SEQ ID NO: 145, and the antisense strand comprises the nucleotide sequence shown as SEQ ID NO: 146; alternatively, the sense strand comprises the sequence set forth as SEQ ID NO: 171, and the antisense strand comprises the nucleotide sequence shown in SEQ ID NO: 172; alternatively, the sense strand comprises the sequence set forth as SEQ ID NO: 177, and the antisense strand comprises the nucleotide sequence shown as SEQ ID NO: 178; alternatively, the sense strand comprises the sequence set forth as SEQ ID NO: 185, and the antisense strand comprises the nucleotide sequence shown in SEQ ID NO: 186; alternatively, the sense strand comprises the sequence set forth as SEQ ID NO: 197, and the antisense strand comprises the nucleotide sequence shown as SEQ ID NO: 198;

wherein,

sense strand 5'-AGGGAUUGUACAGAAAGUGUG-3' (SEQ ID NO: 5),

antisense strand 5'-CACUUUCUGUACAAUCCCUUU-3' (SEQ ID NO: 6);

sense strand 5'-GGUGAUUGUGAAGAAGAAGAG-3' (SEQ ID NO: 9),

antisense strand 5'-CUUCUUCUUCACAAUCACCUU-3' (SEQ ID NO: 10);

sense strand 5'-AAUCCAUCAUUGCCUACACUA-3' (SEQ ID NO: 57),

antisense strand 5'-CAUAGUGUAGGCAAUGAUGGA-3' (SEQ ID NO: 58);

sense strand 5'-AAACCGUGCUUUAACUGGAAU-3' (SEQ ID NO: 59),

antisense strand 5'-CUAUUCCAGUUAAAGCACGGU-3' (SEQ ID NO: 60);

sense strand 5'-AAUGGCACACACUGGUUUGUA-3' (SEQ ID NO: 69),

antisense strand 5'-GUUACAAACCAGUGUGUGCCA-3' (SEQ ID NO: 70);

sense strand 5'-CAAGGCCAAACUGUCACUAAG-3' (SEQ ID NO: 123),

antisense strand 5'-UAGUGACAGUUUGGCCUUGUU-3' (SEQ ID NO: 124);

sense strand 5'-GGCUGAUGAAACUCAAGCCUU-3' (SEQ ID NO: 131),

antisense strand 5'-GGCUUGAGUUUCAUCAGCCUU-3' (SEQ ID NO: 132);

sense strand 5'-UCAGCACCUUUAAUUGAAUUG-3' (SEQ ID NO: 145),

antisense strand 5'-AUUCAAUUAAAGGUGCUGAUU-3' (SEQ ID NO: 146);

sense strand 5'-CUUCCUCAAGGAACAACAUUG-3' (SEQ ID NO: 171),

antisense strand 5'-AUGUUGUUCCUUGAGGAAGUU-3' (SEQ ID NO: 172);

sense strand 5'-GAGUGUGUUAGAGGUACAACA-3' (SEQ ID NO: 177),

antisense strand 5'-UUGUACCUCUAACACACUCUU-3' (SEQ ID NO: 178);

sense strand 5'-CUGACUUGCUUUAGCACUCAA-3' (SEQ ID NO: 185),

antisense strand 5'-GAGUGCUAAAGCAAGUCAGUU-3' (SEQ ID NO: 186);

sense strand 5'-CUUGCUGUUGUUGUUUGUAAC-3' (SEQ ID NO: 197),

antisense strand 5'-UACAAACAACAACAGCAAGUU-3' (SEQ ID NO: 198).

Preferably, at least one of the sense strand and the antisense strand of the siRNA, which are complementary to each other, is deleted or added with one or several nucleotides, thereby obtaining an siRNA derivative having the same function as the siRNA.

Preferably, at least a portion of the phosphate-sugar backbone of at least one of the sense strand and the antisense strand, which are complementary to each other, of the siRNA is a phosphorothioate backbone;

or, at least a part of nucleotides of at least one single strand of the sense strand and the antisense strand complementary to each other is subjected to nucleotide substitution or modification;

alternatively, at least one of the sense strand and the antisense strand, which are complementary to each other, is linked to a signaling molecule and/or an active molecule and/or a functional group.

Preferably, the sense strand of the siRNA is SEQ ID NO: 5 nucleotide sequence, wherein the 1 st, 2 nd, 3 rd, 19 th, 20 th and 21 st nucleotides are all 2' -O-methyl ribonucleotide, and the antisense strand of the siRNA is SEQ ID NO: the 1 st, 2 nd, 3 rd, 19 th, 20 th and 21 th nucleotides of the 6 th nucleotide sequence are all 2' -O-methyl ribonucleotides;

alternatively, the sense strand of the siRNA SEQ ID NO: 9 nucleotide sequence, wherein the 1 st, 2 nd, 3 rd, 19 th, 20 th and 21 st nucleotides are all 2' -O-methyl ribonucleotide, and the antisense strand of the siRNA is SEQ ID NO: 10 nucleotide sequence of 1, 2, 3, 19, 20, 21 nucleotides are all 2' -O-methyl ribonucleotide;

alternatively, the sense strand of the siRNA SEQ ID NO: the nucleotides 1, 2, 3, 19, 20 and 21 of the 57 nucleotide sequence are all 2' -O-methyl ribonucleotide, and the antisense strand of the siRNA is SEQ ID NO: the 1 st, 2 nd, 3 rd, 19 th, 20 th and 21 th nucleotides of the 58 nucleotide sequence are all 2' -O-methyl ribonucleotides;

alternatively, the sense strand of the siRNA SEQ ID NO: 59 nucleotide sequence of 2' -O-methyl ribonucleotide in 1 st, 2 nd, 3 rd, 19 th, 20 th, 21 st nucleotide, the antisense strand of said siRNA SEQ ID NO: the 1 st, 2 nd, 3 rd, 19 th, 20 th and 21 th nucleotides of the 60 th nucleotide sequence are all 2' -O-methyl ribonucleotides;

alternatively, the sense strand of the siRNA SEQ ID NO: 69 nucleotide sequence of 2' -O-methyl ribonucleotide at the 1 st, 2 nd, 3 rd, 19 th, 20 th and 21 st nucleotides, wherein the antisense strand of the siRNA is SEQ ID NO: the 1 st, 2 nd, 3 rd, 19 th, 20 th and 21 th nucleotides of the 70 th nucleotide sequence are all 2' -O-methyl ribonucleotides;

alternatively, the sense strand of the siRNA SEQ ID NO: 123, and 2' -O-methyl ribonucleotide in the 1 st, 2 nd, 3 rd, 19 th, 20 th and 21 st nucleotides of the siRNA, wherein the antisense strand of the siRNA is SEQ ID NO: the 1 st, 2 nd, 3 rd, 19 th, 20 th and 21 st nucleotides of the 124 th nucleotide sequence are all 2' -O-methyl ribonucleotides;

alternatively, the sense strand of the siRNA SEQ ID NO: 131 nucleotide sequence of 2' -O-methyl ribonucleotide at 1 st, 2 nd, 3 rd, 19 th, 20 th and 21 st nucleotides, wherein the antisense strand of the siRNA is SEQ ID NO: the 1 st, 2 nd, 3 rd, 19 th, 20 th and 21 th nucleotides of the 132 nucleotide sequence are all 2' -O-methyl ribonucleotides;

alternatively, the sense strand of the siRNA SEQ ID NO: 145 nucleotide sequence is 2' -O-methyl ribonucleotide at nucleotides 1, 2, 3, 19, 20, 21, and the antisense strand of the siRNA is SEQ ID NO: the 1 st, 2 nd, 3 rd, 19 th, 20 th and 21 th nucleotides of the 146 nucleotide sequence are all 2' -O-methyl ribonucleotides;

alternatively, the sense strand of the siRNA SEQ ID NO: 171 of the siRNA, wherein the nucleotides at positions 1, 2, 3, 19, 20 and 21 are all 2' -O-methyl ribonucleotides, and the antisense strand of the siRNA is SEQ ID NO: the 1 st, 2 nd, 3 rd, 19 th, 20 th and 21 th nucleotides of the 172 nucleotide sequence are all 2' -O-methyl ribonucleotides;

alternatively, the sense strand of the siRNA SEQ ID NO: the 1 st, 2 nd, 3 rd, 19 th, 20 th and 21 st nucleotides of the 177 nucleotide sequence are all 2' -O-methyl ribonucleotides, and the antisense strand of the siRNA is SEQ ID NO: 178 nucleotide sequence, nucleotides 1, 2, 3, 19, 20, 21 are all 2' -O-methyl ribonucleotides;

alternatively, the sense strand of the siRNA SEQ ID NO: 185 nucleotide sequence, nucleotides 1, 2, 3, 19, 20, 21 are all 2' -O-methyl ribonucleotides, and the antisense strand of the siRNA is SEQ ID NO: 186 nucleotide sequence of nucleotides 1, 2, 3, 19, 20, 21 are all 2' -O-methyl ribonucleotides;

alternatively, the sense strand of the siRNA SEQ ID NO: 197, nucleotides 1, 2, 3, 19, 20, and 21 of the nucleotide sequence are all 2' -O-methyl ribonucleotides, and the antisense strand of the siRNA is SEQ ID NO: 198 and the 1 st, 2 nd, 3 rd, 19 th, 20 th and 21 th nucleotides of the nucleotide sequence are all 2' -O-methyl ribonucleotides.

Preferably, the phosphate groups between the 1 st, 2 rd and 3 rd nucleotides and the phosphate groups between the 19 th, 20 th and 21 st nucleotides of the nucleotide sequence of the sense strand and/or the antisense strand of the siRNA are all phosphorothioate groups.

Preferably, the 5' -end of the sense strand of the siRNA is cholesterol-modified.

The invention also provides application of the siRNA in preparation of a medicament for preventing and/or treating viral pneumonia.

Preferably, the viral pneumonia is novel coronavirus pneumonia COVID-19.

The invention also provides a kit, which comprises the siRNA and has the function of treating COVID-19.

Experiments prove that the siRNA modified by the invention has obvious inhibition effect on the novel coronavirus gene, lays a foundation for clinically treating the novel coronavirus pneumonia, and has great clinical significance and popularization value.

Drawings

FIG. 1 is a map of the dual luciferase reporter plasmid GP-mirGlo plasmid in example 3 of the present invention;

FIG. 2 is a graph showing the results of a dual luciferase assay carried out on the siRNAs numbered as mimicNC, 21si-NS-964, 21si-NS-1989 and 21si-NS-2139 in example 4 of the present invention and the corresponding reporter plasmid numbered as P17;

FIG. 3 is a graph showing the results of a dual luciferase assay experiment conducted on the siRNAs numbered as mimicNC, 21si-NS-2588, 21si-NS-2798 and 21si-NS-2915 in example 4 of the present invention and the corresponding reporter plasmid numbered as P18;

FIG. 4 is a graph showing the results of a dual luciferase assay experiment conducted on the siRNAs numbered as mimicNC, 21si-NS-5890, 21si-NS-6010, 21si-NS-6015 and 21si-NS-6201 in example 4 of the present invention and the corresponding reporter plasmid numbered as P19;

FIG. 5 is a graph showing the results of a dual luciferase assay experiment conducted on the siRNAs numbered as mimicNC, 21si-NS-17211, 21si-NS-17400, and 21si-NS-17447 and the corresponding reporter plasmid numbered as P20 in example 4 of the present invention;

FIG. 6 is a graph showing the results of a dual luciferase assay experiment conducted on the siRNAs numbered as mimicNC, 21si-NS-19760, 21si-NS-19831, and 21si-NS-19897 in example 4 of the present invention and the corresponding reporter plasmid numbered as P21;

FIG. 7 is a graph showing the results of a dual luciferase assay experiment conducted on siRNA's numbered as mimicNC, 21si-SP-88, 21si-SP-241, 21si-SP-337 and 21si-SP-450 in example 4 of the present invention with the corresponding reporter plasmid numbered as P22;

FIG. 8 is a graph showing the results of a dual luciferase assay experiment conducted on siRNA's numbered as mimicNC, 21si-SP-1027, 21si-SP-1060, 21si-SP-1493, 21si-SP-1547, 21si-SP-1816 and 21si-SP-1932 and a corresponding reporter plasmid numbered as P23 in example 4 of the present invention;

FIG. 9 is a graph showing the results of a dual luciferase assay carried out with siRNA's numbered as mimicNC, 21si-SP-2128, 21si-SP-2069, 21si-SP-2289, 21si-SP-2301 and 21si-SP-2643 and the corresponding reporter plasmid numbered as P24 in example 4 of the present invention;

FIG. 10 is a graph showing the results of a dual luciferase assay experiment conducted on the siRNAs numbered as mimicNC, 21si-SP-2956, 21si-SP-3051, 21si-SP-3292, 21si-SP-3387 and 21si-SP-3584 in example 4 of the present invention and the corresponding reporter plasmid numbered as P25;

FIG. 11 is a graph showing the results of a dual luciferase assay experiment conducted on siRNA's numbered as mimicsNC, 21si-P3-50, 21si-P3-223, 21si-P3-592, 21si-P3-735, 21si-P3-209, 21si-P3-244, 21si-P3-528, 21si-P3-704, 21si-P3-725 and 21si-P3-744 in example 4 of the present invention with a corresponding reporter plasmid numbered as P34;

FIG. 12 is a graph showing the results of a dual luciferase assay experiment conducted on siRNA's numbered as mimicNC, 21si-HY8-152, 21si-HY8-160, 21si-HY8-7, 21si-HY8-22 and 21si-HY8-119 in example 4 of the present invention with the corresponding reporter plasmid numbered as P30;

FIG. 13 is a graph showing the results of a dual luciferase assay experiment conducted on siRNA's numbered as mimicNC, 21si-P8-30, 21si-P8-62, 21si-P8-95, 21si-P8-98, 21si-P8-269 and 21si-P8-164 in example 4 of the present invention with a corresponding reporter plasmid numbered as P33;

FIG. 14 is a graph showing the results of a dual luciferase assay experiment conducted on siRNA's numbered as mimicNC, 21si-P10b-203, 21si-P10b-256, 21si-P10b-275, 21si-P10b-80, 21si-P10b-103, 21si-P10b-158, 21si-P10b-27, 21si-P10b-38 and 21si-P10b-95 in example 4 of the present invention with the corresponding reporter plasmid numbered as P29;

FIG. 15 is a graph showing the results of a dual luciferase assay experiment conducted on siRNA's numbered as mimicNC, 21si-EP-20, 21si-EP-83, 21si-EP-96 and 21si-EP-124 in example 4 of the present invention with the corresponding reporter plasmid numbered as P26;

FIG. 16 is a graph showing the results of a dual luciferase assay experiment conducted on siRNA's numbered as mimicsNC, 21si-MP-237, 21si-MP-13, 21si-MP-42, 21si-MP-249, 21si-MP-484, 21si-MP-501, 21si-MP-56, 21si-MP-220, 21si-MP-410 and 21si-MP-61 in example 4 of the present invention with the corresponding reporter plasmid numbered as P27;

FIG. 17 is a graph showing the results of a dual luciferase assay experiment conducted on the siRNAs numbered as mimicNC, 21si-P13-163, 21si-P13-199, and 21si-P13-213 in example 4 of the present invention and the corresponding reporter plasmid numbered as P31;

FIG. 18 is a graph showing the results of a dual luciferase assay experiment conducted on the siRNAs numbered as mimicNC, 21si-P14-8, 21si-P14-19 and 21si-P14-82 in example 4 of the present invention and the corresponding reporter plasmid numbered as P32;

FIG. 19 is a graph showing the results of a dual luciferase assay experiment conducted on siRNA's numbered as mimicsNC, 21si-NP-173, 21si-NP-209, 21si-NP-223, 21si-NP-229, 21si-NP-249, 21si-NP-448, 21si-NP-460, 21si-NP-468, 21si-NP-479, 21si-NP-698, 21si-NP-722, 21si-NP-745, 21si-NP-781, 21si-NP-796, 21si-NP-1123, and 21si-NP-1162 in example 4 of the present invention and a corresponding reporter plasmid numbered as P28.

Detailed Description

The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.

Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.

The following non-limiting examples will allow those skilled in the art to better understand the present invention.

Any person skilled in the art can substitute or change the technical scheme and concept of the present invention within the careless scope of the present invention.

The 293T cell line used in the examples below was purchased from the classical collection of cells of the Central institute of technology.

Example 1

Synthesis of Small interfering nucleic acids

A novel coronavirus (SARS-CoV-2) genome (Genbank accession No. MN988668.1) (SEQ ID NO: 227) with a relatively conserved sequence was selected as a template. Respectively aiming at the conserved regions of SARS-CoV-2 gene, selecting nucleotide sequence of 21bp, and designing small interfering nucleic acid (siRNA).

The siRNA designed in this example was synthesized by shanghai gimar pharmaceutical technology ltd, and the sequences of the synthesized siRNA are shown in table 1.

TABLE 1

As shown in table 1, the present example also provides a sense strand nucleotide sequence as shown in seq id no: 207, the nucleotide sequence of the antisense strand is shown as SEQ ID NO: 208, M-mimicsNC as a negative control, which is an unrelated sequence without a target site corresponding to a novel coronavirus (SARS-CoV-2) gene.

Example 2

Synthesis of modified Small interfering nucleic acids

The modified siRNA shown in Table 2 is obtained by chemically modifying the sense strand and the antisense strand of the sequence in Table 1, namely, the 2' hydroxyl group of the pentose group in the nucleotide residues of the 1 st, 2 nd, 3 rd, 19 th, 20 th and 21 st bases of the sense strand is modified by methoxy, the phosphate group between the 1 st, 2 nd and 3 rd bases of the sense strand is phosphorothioate group, and the phosphate group between the 19 th, 20 th and 21 st bases of the sense strand is phosphorothioate group; the 2' hydroxyl of pentose group in the nucleotide residues of 1 st, 2 nd, 3 rd, 19 th, 20 th and 21 st bases of antisense chain is modified by methoxy, the phosphate group between 1 st, 2 nd and 3 rd bases of antisense chain is phosphorothioate group, the phosphate group between 19 th, 20 th and 21 st bases of antisense chain is phosphorothioate group; the siRNA designed in this example was synthesized by shanghai gimar pharmaceutical technology ltd, and cholesterol was linked to the 5' end of the sense strand of siRNA for modification.

TABLE 2

Wherein m represents a pentose group in the nucleotide residue on the left side as a 2' -methoxyribosyl group, and s represents a phosphate group between deoxyribonucleotide residues on the left and right sides thereof as a phosphorothioate group.

Example 3

Construction of dual-luciferase reporter plasmid

The dual-luciferase reporter plasmid used in this example was GP-mirGlo plasmid (the sequence of the empty vector is shown in the specification), purchased from Promega, and the map information is shown in FIG. 1. A dual-luciferase reporter plasmid, as shown in Table 3, was prepared by inserting a novel coronavirus (SARS-CoV-2) genome sequence into the GP-mirTLO plasmid.

TABLE 3

Example 4

Cell transfection

1.293T cells were cultured in 10cm dishes to 80-90% confluency, the culture broth was decanted and the cells were washed twice with 2ml PBS.

2. Adding 2ml of Trypsin-EDTAsolution, mixing uniformly, and standing at 37 ℃ for 1 minute.

3. The pancreatin solution was carefully aspirated, 2ml of DMEM medium containing 10% FBS was added, and the cells were pipetted to form a single cell suspension.

4. Counting with a hemocytometer, diluting the cells to 1X 10⁶Cells/ml. By 5X 10⁵Inoculating 12-well plate with cell/well concentration, mixing well at 37 deg.C with 5% CO₂The culture was carried out for 24 hours.

5. Each 1OD₂₆₀120 μ l DEPC-H for siRNA₂O dissolved to a final concentration of about 20. mu.M.

6. Adding 100 μ l serum-free DMEM into 1.5ml EP tube, adding 7ul siRNA, adding 1.2ug of corresponding dual-fluorescence report carrier, and mixing; another 1.5ml EP tube was added with 100. mu.l serum-free DMEM, 4. mu.l Lipofectamine2000, mixed well, left at room temperature for 5 minutes, then mixed and left at room temperature for 20 minutes.

Grouping was performed according to the correspondence between plasmids and siRNA molecules, as shown in Table 2.

Each group was done 3 more wells, averaged, and tested at time point 24/48h, once.

The culture medium was aspirated from the 12-well plate, and the transfection mixture was added dropwise to the 12-well plate, mixed well, and incubated in an incubator for 5 hours.

7. The transfection solution was aspirated off, and 500ul of DMEM medium containing 10% FBS was added. 5% CO at 37 ℃₂The culture was continued for 24 and 48 hours, and the samples were collected.

Dual luciferase systemic detection

The Dual-Luciferase assay was performed using the Dual-Luciferase reporter assay kit (promega) (see instructions for specific methods of use).

Preparation of reagents:

1. preparation of passive lysis buffer 1 × PLB: 1 volume of 5XpassiveLysis buffer (PLB) was added to 4 volumes of distilled water. And (4) uniformly mixing. Storing at 4 ℃ for later use.

2. LAR II, dissolving the freeze-dried powder of LuciferaseAssaySubstrate by LuciferaseAssayBuffer II. Store at-20 ℃ until use.

3. Preparation of

Reagent (prepared as used): according to the required dosage of the experiment, a certain amount of the medicine is taken

Added in corresponding amount

In Buffer, the final concentration of the seed is 1 × concentration for use.

A detection step:

1. the culture medium in the 12-well plate was decanted and the cells were washed twice with 500ul PBS.

2. 1 XPLB 300ul was added to the culture wells. Passive lysis of cells: the plate was gently shaken at room temperature for 15 minutes and the lysate was transferred to the assay plate. 100ul per well, 3 wells per experimental design.

3. Turning on a TecanM1000 microplate reader, preheating and selecting a dual-luciferase detection system.

4. Add 10ul LARII reagent to each well, select 1-2 seconds delay, read 5-10, detect firefly luciferase activity on the microplate reader.

5. The test plate is removed and added to each well

Reagent, selecting 1-2 seconds delay, reading 5-10, detecting renilla luciferase activity on an enzyme labeling instrument.

6. And (5) counting and analyzing results.

The results of the dual luciferase assay are shown in FIGS. 2-19, and the results show that siRNAs numbered 21-si-SP-2069, 21-si-SP-2289, 21-si-SP-3292, 21-si-P3-244, 21-si-P8-62, 21-si-P8-164, 21-si-P10B-158, 21-si-P14-19, 21-si-NP-1123, 21-si-NS-2139 and 21-si-NS-2798 can effectively inhibit the expression of novel coronavirus genes.

The present invention has been described in detail above. It will be apparent to those skilled in the art that the invention can be practiced in a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the invention and without undue experimentation. While the invention has been described with reference to specific embodiments, it will be appreciated that the invention can be further modified. In general, the invention is intended to encompass any variations, uses, or adaptations of the invention following, in general, the principles of the invention, including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains.

Sequence listing

<110> Suzhou Jima Gene GmbH

SHANGHAI GENEPHARMA Co.,Ltd.

<120> small interfering nucleic acid for inhibiting novel coronavirus, composition and application

<160> 435

<170> SIPOSequenceListing 1.0

<210> 1

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 1

gugugaucau uguggugaaa c 21

<210> 2

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 2

uucaccacaa ugaucacacu u 21

<210> 3

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 3

uugucaccug ugcaaaggaa a 21

<210> 4

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 4

uccuuugcac aggugacaau u 21

<210> 5

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 5

agggauugua cagaaagugu g 21

<210> 6

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 6

cacuuucugu acaaucccuu u 21

<210> 7

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 7

cucgguacag aaguaaauga g 21

<210> 8

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 8

cauuuacuuc uguaccgagu u 21

<210> 9

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 9

ggugauugug aagaagaaga g 21

<210> 10

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 10

cuucuucuuc acaaucaccu u 21

<210> 11

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 11

gaagagcaag aagaagauug g 21

<210> 12

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 12

aaucuucuuc uugcucuucu u 21

<210> 13

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 13

uggugaugug guggcuauug a 21

<210> 14

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 14

aauagccacc acaucaccau u 21

<210> 15

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 15

accaaauacc ugguguauac g 21

<210> 16

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 16

uauacaccag guauuugguu u 21

<210> 17

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 17

auaccuggug uauacguugu c 21

<210> 18

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 18

caacguauac accagguauu u 21

<210> 19

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 19

cuaccgaagu uguaggagac a 21

<210> 20

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 20

ucuccuacaa cuucgguagu u 21

<210> 21

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 21

gggcacacua gaaccagaau a 21

<210> 22

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 22

uucugguucu agugugcccu u 21

<210> 23

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 23

ggguguuauc acgcaugaug u 21

<210> 24

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 24

aucaugcgug auaacacccu u 21

<210> 25

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 25

uaggcguggu aagagaauuc c 21

<210> 26

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 26

aauucucuua ccacgccuau u 21

<210> 27

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 27

augcccguaa ugguguucuu a 21

<210> 28

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 28

agaacaccau uacgggcauu u 21

<210> 29

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 29

gcuagucuua auggagucac a 21

<210> 30

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 30

ugacuccauu aagacuagcu u 21

<210> 31

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 31

guugauggug uuguccaaca a 21

<210> 32

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 32

guuggacaac accaucaacu u 21

<210> 33

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 33

uucuuucaca cgugguguuu a 21

<210> 34

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 34

aacaccacgu gugaaagaau u 21

<210> 35

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 35

cccuguccua ccauuuaaug a 21

<210> 36

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 36

auuaaauggu aggacagggu u 21

<210> 37

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 37

aagacccagu cccuacuuau u 21

<210> 38

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 38

acaauaagua gggacugggu c 21

<210> 39

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 39

aaguuggaug gaaagugagu u 21

<210> 40

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 40

ugaacucacu uuccauccaa c 21

<210> 41

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 41

aaguguacgu ugaaauccuu c 21

<210> 42

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 42

gugaaggauu ucaacguaca c 21

<210> 43

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 43

aacgccacca gauuugcauc u 21

<210> 44

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 44

acagaugcaa aucugguggc g 21

<210> 45

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 45

aacaggaaga gaaucagcaa c 21

<210> 46

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 46

caguugcuga uucucuuccu g 21

<210> 47

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 47

aacccacuaa ugguguuggu u 21

<210> 48

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 48

guaaccaaca ccauuagugg g 21

<210> 49

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 49

aacuucuaca ugcaccagca a 21

<210> 50

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 50

aguugcuggu gcauguagaa g 21

<210> 51

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 51

aaccagguug cuguucuuua u 21

<210> 52

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 52

ugauaaagaa cagcaaccug g 21

<210> 53

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 53

aacacgugca ggcuguuuaa u 21

<210> 54

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 54

cuauuaaaca gccugcacgu g 21

<210> 55

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 55

cucuauugcc auacccacaa a 21

<210> 56

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 56

uguggguaug gcaauagagu u 21

<210> 57

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 57

aauccaucau ugccuacacu a 21

<210> 58

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 58

cauaguguag gcaaugaugg a 21

<210> 59

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 59

aaaccgugcu uuaacuggaa u 21

<210> 60

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 60

cuauuccagu uaaagcacgg u 21

<210> 61

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 61

aacuggaaua gcuguugaac a 21

<210> 62

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 62

cuuguucaac agcuauucca g 21

<210> 63

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 63

aaucacuucu gguuggaccu u 21

<210> 64

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 64

caaaggucca accagaagug a 21

<210> 65

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 65

aaaguugagg cugaagugca a 21

<210> 66

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 66

auuugcacuu cagccucaac u 21

<210> 67

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 67

aaucagagcu ucugcuaauc u 21

<210> 68

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 68

caagauuagc agaagcucug a 21

<210> 69

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 69

aauggcacac acugguuugu a 21

<210> 70

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 70

guuacaaacc agugugugcc a 21

<210> 71

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 71

aauaggaauu gucaacaaca c 21

<210> 72

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 72

cuguguuguu gacaauuccu a 21

<210> 73

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 73

aaucucucau cgaucuccaa g 21

<210> 74

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 74

uucuuggaga ucgaugagag a 21

<210> 75

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 75

gagacaggua cguuaauagu u 21

<210> 76

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 76

cuauuaacgu accugucucu u 21

<210> 77

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 77

guuacacuag ccauccuuac u 21

<210> 78

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 78

uaaggauggc uaguguaacu u 21

<210> 79

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 79

uccuuacugc gcuucgauug u 21

<210> 80

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 80

aaucgaagcg caguaaggau u 21

<210> 81

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 81

cugcugcaau auuguuaacg u 21

<210> 82

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 82

guuaacaaua uugcagcagu u 21

<210> 83

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 83

uugcuaucgc aauggcuugu c 21

<210> 84

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 84

caagccauug cgauagcaau u 21

<210> 85

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 85

cgguacuauu accguugaag a 21

<210> 86

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 86

uucaacggua auaguaccgu u 21

<210> 87

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 87

agcuccuuga acaauggaac c 21

<210> 88

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 88

uuccauuguu caaggagcuu u 21

<210> 89

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 89

auuggaucac cgguggaauu g 21

<210> 90

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 90

auuccaccgg ugauccaauu u 21

<210> 91

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 91

ggaccugccu aaagaaauca c 21

<210> 92

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 92

gauuucuuua ggcagguccu u 21

<210> 93

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 93

ucacuguugc uacaucacga a 21

<210> 94

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 94

cgugauguag caacagugau u 21

<210> 95

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 95

uggaaccuag uaauagguuu c 21

<210> 96

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 96

aaccuauuac uagguuccau u 21

<210> 97

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 97

uuggaucacc gguggaauug c 21

<210> 98

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 98

aauuccaccg gugauccaau u 21

<210> 99

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 99

cucguaaucg gagcugugau c 21

<210> 100

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 100

ucacagcucc gauuacgagu u 21

<210> 101

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 101

ccuaguaaua gguuuccuau u 21

<210> 102

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 102

uaggaaaccu auuacuaggu u 21

<210> 103

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 103

cauggcaagg aagaccuuaa a 21

<210> 104

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 104

uaaggucuuc cuugccaugu u 21

<210> 105

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 105

ggcguuccaa uuaacaccaa u 21

<210> 106

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 106

ugguguuaau uggaacgccu u 21

<210> 107

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 107

caccaauagc aguccagaug a 21

<210> 108

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 108

aucuggacug cuauuggugu u 21

<210> 109

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 109

uagcagucca gaugaccaaa u 21

<210> 110

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 110

uuggucaucu ggacugcuau u 21

<210> 111

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 111

uuggcuacua ccgaagagcu a 21

<210> 112

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 112

gcucuucggu aguagccaau u 21

<210> 113

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 113

uccugcuaac aaugcugcaa u 21

<210> 114

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 114

ugcagcauug uuagcaggau u 21

<210> 115

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 115

ugcugcaauc gugcuacaac u 21

<210> 116

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 116

uuguagcacg auugcagcau u 21

<210> 117

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 117

ucgugcuaca acuuccucaa g 21

<210> 118

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 118

ugaggaaguu guagcacgau u 21

<210> 119

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 119

cuuccucaag gaacaacauu g 21

<210> 120

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 120

auguuguucc uugaggaagu u 21

<210> 121

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 121

augucuggua aaggccaaca a 21

<210> 122

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 122

guuggccuuu accagacauu u 21

<210> 123

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 123

caaggccaaa cugucacuaa g 21

<210> 124

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 124

uagugacagu uuggccuugu u 21

<210> 125

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 125

aucugcugcu gaggcuucua a 21

<210> 126

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 126

agaagccuca gcagcagauu u 21

<210> 127

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 127

acguacugcc acuaaagcau a 21

<210> 128

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 128

ugcuuuagug gcaguacguu u 21

<210> 129

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 129

agcauacaau guaacacaag c 21

<210> 130

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 130

uuguguuaca uuguaugcuu u 21

<210> 131

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 131

ggcugaugaa acucaagccu u 21

<210> 132

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 132

ggcuugaguu ucaucagccu u 21

<210> 133

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 133

acagcaaacu gugacucuuc u 21

<210> 134

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 134

aagagucaca guuugcuguu u 21

<210> 135

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 135

ucacccauuc aguacaucga u 21

<210> 136

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 136

cgauguacug aaugggugau u 21

<210> 137

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 137

uugccaggaa ccuaaauugg g 21

<210> 138

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 138

caauuuaggu uccuggcaau u 21

<210> 139

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 139

ccuaaauugg guagucuugu a 21

<210> 140

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 140

caagacuacc caauuuaggu u 21

<210> 141

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 141

caucaaccau auguaguuga u 21

<210> 142

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 142

caacuacaua ugguugaugu u 21

<210> 143

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 143

cccguguccu auucacuucu a 21

<210> 144

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 144

gaagugaaua ggacacgggu u 21

<210> 145

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 145

ucagcaccuu uaauugaauu g 21

<210> 146

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 146

auucaauuaa aggugcugau u 21

<210> 147

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 147

ucacaacugu agcugcauuu c 21

<210> 148

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 148

aaugcagcua caguugugau u 21

<210> 149

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 149

gcugcauuuc accaagaaug u 21

<210> 150

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 150

auucuuggug aaaugcagcu u 21

<210> 151

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 151

guugaugacc cguguccuau u 21

<210> 152

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 152

uaggacacgg gucaucaacu u 21

<210> 153

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 153

uuagaugaag agcaaccaau g 21

<210> 154

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 154

uugguugcuc uucaucuaau u 21

<210> 155

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 155

agagcaacca auggagauug a 21

<210> 156

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 156

aaucuccauu gguugcucuu u 21

<210> 157

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 157

ucucguugac uuucagguua c 21

<210> 158

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 158

aaccugaaag ucaacgagau u 21

<210> 159

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 159

gguuacuaua gcagagauau u 21

<210> 160

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 160

uaucucugcu auaguaaccu u 21

<210> 161

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 161

ucuaagucac uaacugagaa u 21

<210> 162

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 162

ucucaguuag ugacuuagau u 21

<210> 163

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 163

cauggcaagg aagaccuuaa a 21

<210> 164

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 164

uaaggucuuc cuugccaugu u 21

<210> 165

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 165

ggcguuccaa uuaacaccaa u 21

<210> 166

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 166

ugguguuaau uggaacgccu u 21

<210> 167

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 167

caccaauagc aguccagaug a 21

<210> 168

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 168

aucuggacug cuauuggugu u 21

<210> 169

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 169

ucgugcuaca acuuccucaa g 21

<210> 170

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 170

ugaggaaguu guagcacgau u 21

<210> 171

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 171

cuuccucaag gaacaacauu g 21

<210> 172

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 172

auguuguucc uugaggaagu u 21

<210> 173

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 173

gccucuucuc guuccucauc a 21

<210> 174

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 174

augaggaacg agaagaggcu u 21

<210> 175

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 175

cacucgcuac uugugagcuu u 21

<210> 176

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 176

agcucacaag uagcgagugu u 21

<210> 177

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 177

gaguguguua gagguacaac a 21

<210> 178

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 178

uuguaccucu aacacacucu u 21

<210> 179

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 179

gaaccuugcu cuucuggaac a 21

<210> 180

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 180

uuccagaaga gcaagguucu u 21

<210> 181

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 181

ccuugcucuu cuggaacaua c 21

<210> 182

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 182

auguuccaga agagcaaggu u 21

<210> 183

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 183

gaggaaguuc aagaacuuua c 21

<210> 184

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 184

aaaguucuug aacuuccucu u 21

<210> 185

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 185

cugacuugcu uuagcacuca a 21

<210> 186

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 186

gagugcuaaa gcaagucagu u 21

<210> 187

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 187

ggugaaauca aggaugcuac u 21

<210> 188

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 188

uagcauccuu gauuucaccu u 21

<210> 189

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 189

ggguguucac uuuguuugca a 21

<210> 190

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 190

gcaaacaaag ugaacacccu u 21

<210> 191

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 191

agacuguguu guauuacaca g 21

<210> 192

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 192

guguaauaca acacagucuu u 21

<210> 193

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 193

uucacacaau cgacgguuca u 21

<210> 194

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 194

gaaccgucga uugugugaau u 21

<210> 195

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 195

cuagcacucu ccaagggugu u 21

<210> 196

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 196

cacccuugga gagugcuagu u 21

<210> 197

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 197

cuugcuguug uuguuuguaa c 21

<210> 198

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 198

uacaaacaac aacagcaagu u 21

<210> 199

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 199

guccuauuuc ugaacaugac u 21

<210> 200

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 200

ucauguucag aaauaggacu u 21

<210> 201

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 201

auuguugaug agccugaaga a 21

<210> 202

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 202

cuucaggcuc aucaacaauu u 21

<210> 203

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 203

cauguccaaa uucacacaau c 21

<210> 204

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 204

uugugugaau uuggacaugu u 21

<210> 205

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 205

ucgacgguuc auccggaguu g 21

<210> 206

<211> 21

<212> RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 206

acuccggaug aaccgucgau u 21

<210> 207

<211> 21

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 207

uucuccgaac gugucacgut t 21

<210> 208

<211> 21

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 208

acgugacacg uucggagaat t 21

<210> 209

<211> 1500

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 209

tggggaatgt ccaaattttg tatttccctt aaattccata atcaagacta ttcaaccaag 60

ggttgaaaag aaaaagcttg atggctttat gggtagaatt cgatctgtct atccagttgc 120

gtcaccaaat gaatgcaacc aaatgtgcct ttcaactctc atgaagtgtg atcattgtgg 180

tgaaacttca tggcagacgg gcgattttgt taaagccact tgcgaatttt gtggcactga 240

gaatttgact aaagaaggtg ccactacttg tggttactta ccccaaaatg ctgttgttaa 300

aatttattgt ccagcatgtc acaattcaga agtaggacct gagcatagtc ttgccgaata 360

ccataatgaa tctggcttga aaaccattct tcgtaagggt ggtcgcacta ttgcctttgg 420

aggctgtgtg ttctcttatg ttggttgcca taacaagtgt gcctattggg ttccacgtgc 480

tagcgctaac ataggttgta accatacagg tgttgttgga gaaggttccg aaggtcttaa 540

tgacaacctt cttgaaatac tccaaaaaga gaaagtcaac atcaatattg ttggtgactt 600

taaacttaat gaagagatcg ccattatttt ggcatctttt tctgcttcca caagtgcttt 660

tgtggaaact gtgaaaggtt tggattataa agcattcaaa caaattgttg aatcctgtgg 720

taattttaaa gttacaaaag gaaaagctaa aaaaggtgcc tggaatattg gtgaacagaa 780

atcaatactg agtcctcttt atgcatttgc atcagaggct gctcgtgttg tacgatcaat 840

tttctcccgc actcttgaaa ctgctcaaaa ttctgtgcgt gttttacaga aggccgctat 900

aacaatacta gatggaattt cacagtattc actgagactc attgatgcta tgatgttcac 960

atctgatttg gctactaaca atctagttgt aatggcctac attacaggtg gtgttgttca 1020

gttgacttcg cagtggctaa ctaacatctt tggcactgtt tatgaaaaac tcaaacccgt 1080

ccttgattgg cttgaagaga agtttaagga aggtgtagag tttcttagag acggttggga 1140

aattgttaaa tttatctcaa cctgtgcttg tgaaattgtc ggtggacaaa ttgtcacctg 1200

tgcaaaggaa attaaggaga gtgttcagac attctttaag cttgtaaata aatttttggc 1260

tttgtgtgct gactctatca ttattggtgg agctaaactt aaagccttga atttaggtga 1320

aacatttgtc acgcactcaa agggattgta cagaaagtgt gttaaatcca gagaagaaac 1380

tggcctactc atgcctctaa aagccccaaa agaaattatc ttcttagagg gagaaacact 1440

tcccacagaa gtgttaacag aggaagttgt cttgaaaact ggtgatttac aaccattaga 1500

<210> 210

<211> 500

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 210

aaggttacaa gagtgtgaat atcacttttg aacttgatga aaggattgat aaagtactta 60

atgagaagtg ctctgcctat acagttgaac tcggtacaga agtaaatgag ttcgcctgtg 120

ttgtggcaga tgctgtcata aaaactttgc aaccagtatc tgaattactt acaccactgg 180

gcattgattt agatgagtgg agtatggcta catactactt atttgatgag tctggtgagt 240

ttaaattggc ttcacatatg tattgttctt tctaccctcc agatgaggat gaagaagaag 300

gtgattgtga agaagaagag tttgagccat caactcaata tgagtatggt actgaagatg 360

attaccaagg taaacctttg gaatttggtg ccacttctgc tgctcttcaa cctgaagaag 420

agcaagaaga agattggtta gatgatgata gtcaacaaac tgttggtcaa caagacggca 480

gtgaggacaa tcagacaact 500

<210> 211

<211> 500

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 211

atatcaaatt tgctgatgat ttaaaccagt taactggtta taagaaacct gcttcaagag 60

agcttaaagt tacatttttc cctgacttaa atggtgatgt ggtggctatt gattataaac 120

actacacacc ctcttttaag aaaggagcta aattgttaca taaacctatt gtttggcatg 180

ttaacaatgc aactaataaa gccacgtata aaccaaatac ctggtgtata cgttgtcttt 240

ggagcacaaa accagttgaa acatcaaatt cgtttgatgt actgaagtca gaggacgcgc 300

agggaatgga taatcttgcc tgcgaagatc taaaaccagt ctctgaagaa gtagtggaaa 360

atcctaccat acagaaagac gttcttgagt gtaatgtgaa aactaccgaa gttgtaggag 420

acattatact taaaccagca aataatagtt taaaaattac agaagaggtt ggccacacag 480

atctaatggc tgcttatgta 500

<210> 212

<211> 500

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 212

caatggccac aaattatgat ttgagtgttg tcaatgccag attacgtgct aagcactatg 60

tgtacattgg cgaccctgct caattacctg caccacgcac attgctaact aagggcacac 120

tagaaccaga atatttcaat tcagtgtgta gacttatgaa aactataggt ccagacatgt 180

tcctcggaac ttgtcggcgt tgtcctgctg aaattgttga cactgtgagt gctttggttt 240

atgataataa gcttaaagca cataaagaca aatcagctca atgctttaaa atgttttata 300

agggtgttat cacgcatgat gtttcatctg caattaacag gccacaaata ggcgtggtaa 360

gagaattcct tacacgtaac cctgcttgga gaaaagctgt ctttatttca ccttataatt 420

cacagaatgc tgtagcctca aagattttgg gactaccaac tcaaactgtt gattcatcac 480

agggctcaga atatgactat 500

<210> 213

<211> 440

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 213

tactgtgatc tgggactaca aaagagatgc tccagcacat atatctacta ttggtgtttg 60

ttctatgact gacatagcca agaaaccaac tgaaacgatt tgtgcaccac tcactgtctt 120

ttttgatggt agagttgatg gtcaagtaga cttatttaga aatgcccgta atggtgttct 180

tattacagaa ggtagtgtta aaggtttaca accatctgta ggtcccaaac aagctagtct 240

taatggagtc acattaattg gagaagccgt aaaaacacag ttcaattatt ataagaaagt 300

tgatggtgtt gtccaacaat tacctgaaac ttactttact cagagtagaa atttacaaga 360

atttaaaccc aggagtcaaa tggaaattga tttcttagaa ttagctatgg atgaattcat 420

tgaacggtat aaattagaag 440

<210> 214

<211> 960

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 214

atgtttgttt ttcttgtttt attgccacta gtctctagtc agtgtgttaa tcttacaacc 60

agaactcaat taccccctgc atacactaat tctttcacac gtggtgttta ttaccctgac 120

aaagttttca gatcctcagt tttacattca actcaggact tgttcttacc tttcttttcc 180

aatgttactt ggttccatgc tatacatgtc tctgggacca atggtactaa gaggtttgat 240

aaccctgtcc taccatttaa tgatggtgtt tattttgctt ccactgagaa gtctaacata 300

ataagaggct ggatttttgg tactacttta gattcgaaga cccagtccct acttattgtt 360

aataacgcta ctaatgttgt tattaaagtc tgtgaatttc aattttgtaa tgatccattt 420

ttgggtgttt attaccacaa aaacaacaaa agttggatgg aaagtgagtt cagagtttat 480

tctagtgcga ataattgcac ttttgaatat gtctctcagc cttttcttat ggaccttgaa 540

ggaaaacagg gtaatttcaa aaatcttagg gaatttgtgt ttaagaatat tgatggttat 600

tttaaaatat attctaagca cacgcctatt aatttagtgc gtgatctccc tcagggtttt 660

tcggctttag aaccattggt agatttgcca ataggtatta acatcactag gtttcaaact 720

ttacttgctt tacatagaag ttatttgact cctggtgatt cttcttcagg ttggacagct 780

ggtgctgcag cttattatgt gggttatctt caacctagga cttttctatt aaaatataat 840

gaaaatggaa ccattacaga tgctgtagac tgtgcacttg accctctctc agaaacaaag 900

tgtacgttga aatccttcac tgtagaaaaa ggaatctatc aaacttctaa ctttagagtc 960

<210> 215

<211> 940

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 215

caaccaacag aatctattgt tagatttcct aatattacaa acttgtgccc ttttggtgaa 60

gtttttaacg ccaccagatt tgcatctgtt tatgcttgga acaggaagag aatcagcaac 120

tgtgttgctg attattctgt cctatataat tccgcatcat tttccacttt taagtgttat 180

ggagtgtctc ctactaaatt aaatgatctc tgctttacta atgtctatgc agattcattt 240

gtaattagag gtgatgaagt cagacaaatc gctccagggc aaactggaaa gattgctgat 300

tataattata aattaccaga tgattttaca ggctgcgtta tagcttggaa ttctaacaat 360

cttgattcta aggttggtgg taattataat tacctgtata gattgtttag gaagtctaat 420

ctcaaacctt ttgagagaga tatttcaact gaaatctatc aggccggtag cacaccttgt 480

aatggtgttg aaggttttaa ttgttacttt cctttacaat catatggttt ccaacccact 540

aatggtgttg gttaccaacc atacagagta gtagtacttt cttttgaact tctacatgca 600

ccagcaactg tttgtggacc taaaaagtct actaatttgg ttaaaaacaa atgtgtcaat 660

ttcaacttca atggtttaac aggcacaggt gttcttactg agtctaacaa aaagtttctg 720

cctttccaac aatttggcag agacattgct gacactactg atgctgtccg tgatccacag 780

acacttgaga ttcttgacat tacaccatgt tcttttggtg gtgtcagtgt tataacacca 840

ggaacaaata cttctaacca ggttgctgtt ctttatcagg atgttaactg cacagaagtc 900

cctgttgcta ttcatgcaga tcaacttact cctacttggc 940

<210> 216

<211> 960

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 216

gtgtttattc tacaggttct aatgtttttc aaacacgtgc aggctgttta ataggggctg 60

aacatgtcaa caactcatat gagtgtgaca tacccattgg tgcaggtata tgcgctagtt 120

atcagactca gactaattct cctcggcggg cacgtagtgt agctagtcaa tccatcattg 180

cctacactat gtcacttggt gcagaaaatt cagttgctta ctctaataac tctattgcca 240

tacccacaaa ttttactatt agtgttacca cagaaattct accagtgtct atgaccaaga 300

catcagtaga ttgtacaatg tacatttgtg gtgattcaac tgaatgcagc aatcttttgt 360

tgcaatatgg cagtttttgt acacaattaa accgtgcttt aactggaata gctgttgaac 420

aagacaaaaa cacccaagaa gtttttgcac aagtcaaaca aatttacaaa acaccaccaa 480

ttaaagattt tggtggtttt aatttttcac aaatattacc agatccatca aaaccaagca 540

agaggtcatt tattgaagat ctacttttca acaaagtgac acttgcagat gctggcttca 600

tcaaacaata tggtgattgc cttggtgata ttgctgctag agacctcatt tgtgcacaaa 660

agtttaacgg ccttactgtt ttgccacctt tgctcacaga tgaaatgatt gctcaataca 720

cttctgcact gttagcgggt acaatcactt ctggttggac ctttggtgca ggtgctgcat 780

tacaaatacc atttgctatg caaatggctt ataggtttaa tggtattgga gttacacaga 840

atgttctcta tgagaaccaa aaattgattg ccaaccaatt taatagtgct attggcaaaa 900

ttcaagactc actttcttcc acagcaagtg cacttggaaa acttcaagat gtggtcaacc 960

<210> 217

<211> 962

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 217

aaaatgcaca agctttaaac acgcttgtta aacaacttag ctccaatttt ggtgcaattt 60

caagtgtttt aaatgatatc ctttcacgtc ttgacaaagt tgaggctgaa gtgcaaattg 120

ataggttgat cacaggcaga cttcaaagtt tgcagacata tgtgactcaa caattaatta 180

gagctgcaga aatcagagct tctgctaatc ttgctgctac taaaatgtca gagtgtgtac 240

ttggacaatc aaaaagagtt gatttttgtg gaaagggcta tcatcttatg tccttccctc 300

agtcagcacc tcatggtgta gtcttcttgc atgtgactta tgtccctgca caagaaaaga 360

acttcacaac tgctcctgcc atttgtcatg atggaaaagc acactttcct cgtgaaggtg 420

tctttgtttc aaatggcaca cactggtttg taacacaaag gaatttttat gaaccacaaa 480

tcattactac agacaacaca tttgtgtctg gtaactgtga tgttgtaata ggaattgtca 540

acaacacagt ttatgatcct ttgcaacctg aattagactc attcaaggag gagttagata 600

aatattttaa gaatcataca tcaccagatg ttgatttagg tgacatctct ggcattaatg 660

cttcagttgt aaacattcaa aaagaaattg accgcctcaa tgaggttgcc aagaatttaa 720

atgaatctct catcgatctc caagaacttg gaaagtatga gcagtatata aaatggccat 780

ggtacatttg gctaggtttt atagctggct tgattgccat agtaatggtg acaattatgc 840

tttgctgtat gaccagttgc tgtagttgtc tcaagggctg ttgttcttgt ggatcctgct 900

gcaaatttga tgaagacgac tctgagccag tgctcaaagg agtcaaatta cattacacat 960

aa 962

<210> 218

<211> 228

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 218

atgtactcat tcgtttcgga agagacaggt acgttaatag ttaatagcgt acttcttttt 60

cttgctttcg tggtattctt gctagttaca ctagccatcc ttactgcgct tcgattgtgt 120

gcgtactgct gcaatattgt taacgtgagt cttgtaaaac cttcttttta cgtttactct 180

cgtgttaaaa atctgaattc ttctagagtt cctgatcttc tggtctaa 228

<210> 219

<211> 669

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 219

atggcagatt ccaacggtac tattaccgtt gaagagctta aaaagctcct tgaacaatgg 60

aacctagtaa taggtttcct attccttaca tggatttgtc ttctacaatt tgcctatgcc 120

aacaggaata ggtttttgta tataattaag ttaattttcc tctggctgtt atggccagta 180

actttagctt gttttgtgct tgctgctgtt tacagaataa attggatcac cggtggaatt 240

gctatcgcaa tggcttgtct tgtaggcttg atgtggctca gctacttcat tgcttctttc 300

agactgtttg cgcgtacgcg ttccatgtgg tcattcaatc cagaaactaa cattcttctc 360

aacgtgccac tccatggcac tattctgacc agaccgcttc tagaaagtga actcgtaatc 420

ggagctgtga tccttcgtgg acatcttcgt attgctggac accatctagg acgctgtgac 480

atcaaggacc tgcctaaaga aatcactgtt gctacatcac gaacgctttc ttattacaaa 540

ttgggagctt cgcagcgtgt agcaggtgac tcaggttttg ctgcatacag tcgctacagg 600

attggcaact ataaattaaa cacagaccat tccagtagca gtgacaatat tgctttgctt 660

gtacagtaa 669

<210> 220

<211> 1069

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 220

taaattccct cgaggacaag gcgttccaat taacaccaat agcagtccag atgaccaaat 60

tggctactac cgaagagcta ccagacgaat tcgtggtggt gacggtaaaa tgaaagatct 120

cagtccaaga tggtatttct actacctagg aactgggcca gaagctggac ttccctatgg 180

tgctaacaaa gacggcatca tatgggttgc aactgaggga gccttgaata caccaaaaga 240

tcacattggc acccgcaatc ctgctaacaa tgctgcaatc gtgctacaac ttcctcaagg 300

aacaacattg ccaaaaggct tctacgcaga agggagcaga ggcggcagtc aagcctcttc 360

tcgttcctca tcacgtagtc gcaacagttc aagaaattca actccaggca gcagtagggg 420

aacttctcct gctagaatgg ctggcaatgg cggtgatgct gctcttgctt tgctgctgct 480

tgacagattg aaccagcttg agagcaaaat gtctggtaaa ggccaacaac aacaaggcca 540

aactgtcact aagaaatctg ctgctgaggc ttctaagaag cctcggcaaa aacgtactgc 600

cactaaagca tacaatgtaa cacaagcttt cggcagacgt ggtccagaac aaacccaagg 660

aaattttggg gaccaggaac taatcagaca aggaactgat tacaaacatt ggccgcaaat 720

tgcacaattt gcccccagcg cttcagcgtt cttcggaatg tcgcgcattg gcatggaagt 780

cacaccttcg ggaacgtggt tgacctacac agctgccatc aaattggatg acaaagatcc 840

aaatttcaaa gatcaagtca ttttgctgaa taagcatatt gacgcataca aaacattccc 900

accaacagag cctaaaaagg acaaaaagaa gaaggctgat gaaactcaag ccttaccgca 960

gagacagaag aaacagcaaa ctgtgactct tcttcctgct gcagatttgg atgatttctc 1020

caaacaattg caacaatcca tgagcagtgc tgactcaact caggcctaa 1069

<210> 221

<211> 366

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 221

atgaaatttc ttgttttctt aggaatcatc acaactgtag ctgcatttca ccaagaatgt 60

agtttacagt catgtactca acatcaacca tatgtagttg atgacccgtg tcctattcac 120

ttctattcta aatggtatat tagagtagga gctagaaaat cagcaccttt aattgaattg 180

tgcgtggatg aggctggttc taaatcaccc attcagtaca tcgatatcgg taattataca 240

gtttcctgtt taccttttac aattaattgc caggaaccta aattgggtag tcttgtagtg 300

cgttgttcgt tctatgaaga ctttttagag tatcatgacg ttcgtgttgt tttagatttc 360

atctaa 366

<210> 222

<211> 186

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 222

atgtttcatc tcgttgactt tcaggttact atagcagaga tattactaat tattatgagg 60

acttttaaag tttccatttg gaatcttgat tacatcataa acctcataat taaaaattta 120

tctaagtcac taactgagaa taaatattct caattagatg aagagcaacc aatggagatt 180

gattaa 186

<210> 223

<211> 294

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 223

atggacccca aaatcagcga aatgcacccc gcattacgtt tggtggaccc tcagattcaa 60

ctggcagtaa ccagaatgga gaacgcagtg gggcgcgatc aaaacaacgt cggccccaag 120

gtttacccaa taatactgcg tcttggttca ccgctctcac tcaacatggc aaggaagacc 180

ttaaattccc tcgaggacaa ggcgttccaa ttaacaccaa tagcagtcca gatgaccaaa 240

ttggctacta ccgaagagct accagacgaa ttcgtggtgg tgacggtaaa atga 294

<210> 224

<211> 222

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 224

atgctgcaat cgtgctacaa cttcctcaag gaacaacatt gccaaaaggc ttctacgcag 60

aagggagcag aggcggcagt caagcctctt ctcgttcctc atcacgtagt cgcaacagtt 120

caagaaattc aactccaggc agcagtaggg gaacttctcc tgctagaatg gctggcaatg 180

gcggtgatgc tgctcttgct ttgctgctgc ttgacagatt ga 222

<210> 225

<211> 368

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 225

atgaaaatta ttcttttctt ggcactgata acactcgcta cttgtgagct ttatcactac 60

caagagtgtg ttagaggtac aacagtactt ttaaaagaac cttgctcttc tggaacatac 120

gagggcaatt caccatttca tcctctagct gataacaaat ttgcactgac ttgctttagc 180

actcaatttg cttttgcttg tcctgacggc gtaaaacacg tctatcagtt acgtgccaga 240

tcagtttcac ctaaactgtt catcagacaa gaggaagttc aagaacttta ctctccaatt 300

tttcttattg ttgcggcaat agtgtttata acactttgct tcacactcaa aagaaagaca 360

gaatgatt 368

<210> 226

<211> 828

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 226

atggatttgt ttatgagaat cttcacaatt ggaactgtaa ctttgaagca aggtgaaatc 60

aaggatgcta ctccttcaga ttttgttcgc gctactgcaa cgataccgat acaagcctca 120

ctccctttcg gatggcttat tgttggcgtt gcacttcttg ctgtttttca gagcgcttcc 180

aaaatcataa ccctcaaaaa gagatggcaa ctagcactct ccaagggtgt tcactttgtt 240

tgcaacttgc tgttgttgtt tgtaacagtt tactcacacc ttttgctcgt tgctgctggc 300

cttgaagccc cttttctcta tctttatgct ttagtctact tcttgcagag tataaacttt 360

gtaagaataa taatgaggct ttggctttgc tggaaatgcc gttccaaaaa cccattactt 420

tatgatgcca actattttct ttgctggcat actaattgtt acgactattg tataccttac 480

aatagtgtaa cttcttcaat tgtcattact tcaggtgatg gcacaacaag tcctatttct 540

gaacatgact accagattgg tggttatact gaaaaatggg aatctggagt aaaagactgt 600

gttgtattac acagttactt cacttcagac tattaccagc tgtactcaac tcaattgagt 660

acagacactg gtgttgaaca tgttaccttc ttcatctaca ataaaattgt tgatgagcct 720

gaagaacatg tccaaattca cacaatcgac ggttcatccg gagttgttaa tccagtaatg 780

gaaccaattt atgatgaacc gacgacgact actagcgtgc ctttgtaa 828

<210> 227

<211> 29881

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 227

ttaaaggttt ataccttccc aggtaacaaa ccaaccaact ttcgatctct tgtagatctg 60

ttctctaaac gaactttaaa atctgtgtgg ctgtcactcg gctgcatgct tagtgcactc 120

acgcagtata attaataact aattactgtc gttgacagga cacgagtaac tcgtctatct 180

tctgcaggct gcttacggtt tcgtccgtgt tgcagccgat catcagcaca tctaggtttc 240

gtccgggtgt gaccgaaagg taagatggag agccttgtcc ctggtttcaa cgagaaaaca 300

cacgtccaac tcagtttgcc tgttttacag gttcgcgacg tgctcgtacg tggctttgga 360

gactccgtgg aggaggtctt atcagaggca cgtcaacatc ttaaagatgg cacttgtggc 420

ttagtagaag ttgaaaaagg cgttttgcct caacttgaac agccctatgt gttcatcaaa 480

cgttcggatg ctcgaactgc acctcatggt catgttatgg ttgagctggt agcagaactc 540

gaaggcattc agtacggtcg tagtggtgag acacttggtg tccttgtccc tcatgtgggc 600

gaaataccag tggcttaccg caaggttctt cttcgtaaga acggtaataa aggagctggt 660

ggccatagtt acggcgccga tctaaagtca tttgacttag gcgacgagct tggcactgat 720

ccttatgaag attttcaaga aaactggaac actaaacata gcagtggtgt tacccgtgaa 780

ctcatgcgtg agcttaacgg aggggcatac actcgctatg tcgataacaa cttctgtggc 840

cctgatggct accctcttga gtgcattaaa gaccttctag cacgtgctgg taaagcttca 900

tgcactttgt ccgaacaact ggactttatt gacactaaga ggggtgtata ctgctgccgt 960

gaacatgagc atgaaattgc ttggtacacg gaacgttctg aaaagagcta tgaattgcag 1020

acaccttttg aaattaaatt ggcaaagaaa tttgacacct tcaatgggga atgtccaaat 1080

tttgtatttc ccttaaattc cataatcaag actattcaac caagggttga aaagaaaaag 1140

cttgatggct ttatgggtag aattcgatct gtctatccag ttgcgtcacc aaatgaatgc 1200

aaccaaatgt gcctttcaac tctcatgaag tgtgatcatt gtggtgaaac ttcatggcag 1260

acgggcgatt ttgttaaagc cacttgcgaa ttttgtggca ctgagaattt gactaaagaa 1320

ggtgccacta cttgtggtta cttaccccaa aatgctgttg ttaaaattta ttgtccagca 1380

tgtcacaatt cagaagtagg acctgagcat agtcttgccg aataccataa tgaatctggc 1440

ttgaaaacca ttcttcgtaa gggtggtcgc actattgcct ttggaggctg tgtgttctct 1500

tatgttggtt gccataacaa gtgtgcctat tgggttccac gtgctagcgc taacataggt 1560

tgtaaccata caggtgttgt tggagaaggt tccgaaggtc ttaatgacaa ccttcttgaa 1620

atactccaaa aagagaaagt caacatcaat attgttggtg actttaaact taatgaagag 1680

atcgccatta ttttggcatc tttttctgct tccacaagtg cttttgtgga aactgtgaaa 1740

ggtttggatt ataaagcatt caaacaaatt gttgaatcct gtggtaattt taaagttaca 1800

aaaggaaaag ctaaaaaagg tgcctggaat attggtgaac agaaatcaat actgagtcct 1860

ctttatgcat ttgcatcaga ggctgctcgt gttgtacgat caattttctc ccgcactctt 1920

gaaactgctc aaaattctgt gcgtgtttta cagaaggccg ctataacaat actagatgga 1980

atttcacagt attcactgag actcattgat gctatgatgt tcacatctga tttggctact 2040

aacaatctag ttgtaatggc ctacattaca ggtggtgttg ttcagttgac ttcgcagtgg 2100

ctaactaaca tctttggcac tgtttatgaa aaactcaaac ccgtccttga ttggcttgaa 2160

gagaagttta aggaaggtgt agagtttctt agagacggtt gggaaattgt taaatttatc 2220

tcaacctgtg cttgtgaaat tgtcggtgga caaattgtca cctgtgcaaa ggaaattaag 2280

gagagtgttc agacattctt taagcttgta aataaatttt tggctttgtg tgctgactct 2340

atcattattg gtggagctaa acttaaagcc ttgaatttag gtgaaacatt tgtcacgcac 2400

tcaaagggat tgtacagaaa gtgtgttaaa tccagagaag aaactggcct actcatgcct 2460

ctaaaagccc caaaagaaat tatcttctta gagggagaaa cacttcccac agaagtgtta 2520

acagaggaag ttgtcttgaa aactggtgat ttacaaccat tagaacaacc tactagtgaa 2580

gctgttgaag ctccattggt tggtacacca gtttgtatta acgggcttat gttgctcgaa 2640

atcaaagaca cagaaaagta ctgtgccctt gcacctaata tgatggtaac aaacaatacc 2700

ttcacactca aaggcggtgc accaacaaag gttacttttg gtgatgacac tgtgatagaa 2760

gtgcaaggtt acaagagtgt gaatatcact tttgaacttg atgaaaggat tgataaagta 2820

cttaatgaga agtgctctgc ctatacagtt gaactcggta cagaagtaaa tgagttcgcc 2880

tgtgttgtgg cagatgctgt cataaaaact ttgcaaccag tatctgaatt acttacacca 2940

ctgggcattg atttagatga gtggagtatg gctacatact acttatttga tgagtctggt 3000

gagtttaaat tggcttcaca tatgtattgt tctttctacc ctccagatga ggatgaagaa 3060

gaaggtgatt gtgaagaaga agagtttgag ccatcaactc aatatgagta tggtactgaa 3120

gatgattacc aaggtaaacc tttggaattt ggtgccactt ctgctgctct tcaacctgaa 3180

gaagagcaag aagaagattg gttagatgat gatagtcaac aaactgttgg tcaacaagac 3240

ggcagtgagg acaatcagac aactactatt caaacaattg ttgaggttca acctcaatta 3300

gagatggaac ttacaccagt tgttcagact attgaagtga atagttttag tggttattta 3360

aaacttactg acaatgtata cattaaaaat gcagacattg tggaagaagc taaaaaggta 3420

aaaccaacag tggttgttaa tgcagccaat gtttacctta aacatggagg aggtgttgca 3480

ggagccttaa ataaggctac taacaatgcc atgcaagttg aatctgatga ttacatagct 3540

actaatggac cacttaaagt gggtggtagt tgtgttttaa gcggacacaa tcttgctaaa 3600

cactgtcttc atgttgtcgg cccaaatgtt aacaaaggtg aagacattca acttcttaag 3660

agtgcttatg aaaattttaa tcagcacgaa gttctacttg caccattatt atcagctggt 3720

atttttggtg ctgaccctat acattcttta agagtttgtg tagatactgt tcgcacaaat 3780

gtctacttag ctgtctttga taaaaatctc tatgacaaac ttgtttcaag ctttttggaa 3840

atgaagagtg aaaagcaagt tgaacaaaag atcgctgaga ttcctaaaga ggaagttaag 3900

ccatttataa ctgaaagtaa accttcagtt gaacagagaa aacaagatga taagaaaatc 3960

aaagcttgtg ttgaagaagt tacaacaact ctggaagaaa ctaagttcct cacagaaaac 4020

ttgttacttt atattgacat taatggcaat cttcatccag attctgccac tcttgttagt 4080

gacattgaca tcactttctt aaagaaagat gctccatata tagtgggtga tgttgttcaa 4140

gagggtgttt taactgctgt ggttatacct actaaaaagg ctggtggcac tactgaaatg 4200

ctagcgaaag ctttgagaaa agtgccaaca gacaattata taaccactta cccgggtcag 4260

ggtttaaatg gttacactgt agaggaggca aagacagtgc ttaaaaagtg taaaagtgcc 4320

ttttacattc taccatctat tatctctaat gagaagcaag aaattcttgg aactgtttct 4380

tggaatttgc gagaaatgct tgcacatgca gaagaaacac gcaaattaat gcctgtctgt 4440

gtggaaacta aagccatagt ttcaactata cagcgtaaat ataagggtat taaaatacaa 4500

gagggtgtgg ttgattatgg tgctagattt tacttttaca ccagtaaaac aactgtagcg 4560

tcacttatca acacacttaa cgatctaaat gaaactcttg ttacaatgcc acttggctat 4620

gtaacacatg gcttaaattt ggaagaagct gctcggtata tgagatctct caaagtgcca 4680

gctacagttt ctgtttcttc acctgatgct gttacagcgt ataatggtta tcttacttct 4740

tcttctaaaa cacctgaaga acattttatt gaaaccatct cacttgctgg ttcctataaa 4800

gattggtcct attctggaca atctacacaa ctaggtatag aatttcttaa gagaggtgat 4860

aaaagtgtat attacactag taatcctacc acattccacc tagatggtga agttatcacc 4920

tttgacaatc ttaagacact tctttctttg agagaagtga ggactattaa ggtgtttaca 4980

acagtagaca acattaacct ccacacgcaa gttgtggaca tgtcaatgac atatggacaa 5040

cagtttggtc caacttattt ggatggagct gatgttacta aaataaaacc tcataattca 5100

catgaaggta aaacatttta tgttttacct aatgatgaca ctctacgtgt tgaggctttt 5160

gagtactacc acacaactga tcctagtttt ctgggtaggt acatgtcagc attaaatcac 5220

actaaaaagt ggaaataccc acaagttaat ggtttaactt ctattaaatg ggcagataac 5280

aactgttatc ttgccactgc attgttaaca ctccaacaaa tagagttgaa gtttaatcca 5340

cctgctctac aagatgctta ttacagagca agggctggtg aagctgctaa cttttgtgca 5400

cttatcttag cctactgtaa taagacagta ggtgagttag gtgatgttag agaaacaatg 5460

agttacttgt ttcaacatgc caatttagat tcttgcaaaa gagtcttgaa cgtggtgtgt 5520

aaaacttgtg gacaacagca gacaaccctt aagggtgtag aagctgttat gtacatgggc 5580

acactttctt atgaacaatt taagaaaggt gttcagatac cttgtacgtg tggtaaacaa 5640

gctacaaaat atctagtaca acaggagtca ccttttgtta tgatgtcagc accacctgct 5700

cagtatgaac ttaagcatgg tacatttact tgtgctagtg agtacactgg taattaccag 5760

tgtggtcact ataaacatat aacttctaaa gaaactttgt attgcataga cggtgcttta 5820

cttacaaagt cctcagaata caaaggtcct attacggatg ttttctacaa agaaaacagt 5880

tacacaacaa ccataaaacc agttacttat aaattggatg gtgttgtttg tacagaaatt 5940

gaccctaagt tggacaatta ttataagaaa gacaattctt atttcacaga gcaaccaatt 6000

gatcttgtac caaaccaacc atatccaaac gcaagcttcg ataattttaa gtttgtatgt 6060

gataatatca aatttgctga tgatttaaac cagttaactg gttataagaa acctgcttca 6120

agagagctta aagttacatt tttccctgac ttaaatggtg atgtggtggc tattgattat 6180

aaacactaca caccctcttt taagaaagga gctaaattgt tacataaacc tattgtttgg 6240

catgttaaca atgcaactaa taaagccacg tataaaccaa atacctggtg tatacgttgt 6300

ctttggagca caaaaccagt tgaaacatca aattcgtttg atgtactgaa gtcagaggac 6360

gcgcagggaa tggataatct tgcctgcgaa gatctaaaac cagtctctga agaagtagtg 6420

gaaaatccta ccatacagaa agacgttctt gagtgtaatg tgaaaactac cgaagttgta 6480

ggagacatta tacttaaacc agcaaataat agtttaaaaa ttacagaaga ggttggccac 6540

acagatctaa tggctgctta tgtagacaat tctagtctta ctattaagaa acctaatgaa 6600

ttatctagag tattaggttt gaaaaccctt gctactcatg gtttagctgc tgttaatagt 6660

gtcccttggg atactatagc taattatgct aagccttttc ttaacaaagt tgttagtaca 6720

actactaaca tagttacacg gtgtttaaac cgtgtttgta ctaattatat gccttatttc 6780

tttactttat tgctacaatt gtgtactttt actagaagta caaattctag aattaaagca 6840

tctatgccga ctactatagc aaagaatact gttaagagtg tcggtaaatt ttgtctagag 6900

gcttcattta attatttgaa gtcacctaat ttttctaaac tgataaatat tataatttgg 6960

tttttactat taagtgtttg cctaggttct ttaatctact caaccgctgc tttaggtgtt 7020

ttaatgtcta atttaggcat gccttcttac tgtactggtt acagagaagg ctatttgaac 7080

tctactaatg tcactattgc aacctactgt actggttcta taccttgtag tgtttgtctt 7140

agtggtttag attctttaga cacctatcct tctttagaaa ctatacaaat taccatttca 7200

tcttttaaat gggatttaac tgcttttggc ttagttgcag agtggttttt ggcatatatt 7260

cttttcacta ggtttttcta tgtacttgga ttggctgcaa tcatgcaatt gtttttcagc 7320

tattttgcag tacattttat tagtaattct tggcttatgt ggttaataat taatcttgta 7380

caaatggccc cgatttcagc tatggttaga atgtacatct tctttgcatc attttattat 7440

gtatggaaaa gttatgtgca tgttgtagac ggttgtaatt catcaacttg tatgatgtgt 7500

tacaaacgta atagagcaac aagagtcgaa tgtacaacta ttgttaatgg tgttagaagg 7560

tccttttatg tctatgctaa tggaggtaaa ggcttttgca aactacacaa ttggaattgt 7620

gttaattgtg atacattctg tgctggtagt acatttatta gtgatgaagt tgcgagagac 7680

ttgtcactac agtttaaaag accaataaat cctactgacc agtcttctta catcgttgat 7740

agtgttacag tgaagaatgg ttccatccat ctttactttg ataaagctgg tcaaaagact 7800

tatgaaagac attctctctc tcattttgtt aacttagaca acctgagagc taataacact 7860

aaaggttcat tgcctattaa tgttatagtt tttgatggta aatcaaaatg tgaagaatca 7920

tctgcaaaat cagcgtctgt ttactacagt cagcttatgt gtcaacctat actgttacta 7980

gatcaggcat tagtgtctga tgttggtgat agtgcggaag ttgcagttaa aatgtttgat 8040

gcttacgtta atacgttttc atcaactttt aacgtaccaa tggaaaaact caaaacacta 8100

gttgcaactg cagaagctga acttgcaaag aatgtgtcct tagacaatgt cttatctact 8160

tttatttcag cagctcggca agggtttgtt gattcagatg tagaaactaa agatgttgtt 8220

gaatgtctta aattgtcaca tcaatctgac atagaagtta ctggcgatag ttgtaataac 8280

tatatgctca cctataacaa agttgaaaac atgacacccc gtgaccttgg tgcttgtatt 8340

gactgtagtg cgcgtcatat taatgcgcag gtagcaaaaa gtcacaacat tgctttgata 8400

tggaacgtta aagatttcat gtcattgtct gaacaactac gaaaacaaat acgtagtgct 8460

gctaaaaaga ataacttacc ttttaagttg acatgtgcaa ctactagaca agttgttaat 8520

gttgtaacaa caaagatagc acttaagggt ggtaaaattg ttaataattg gttgaagcag 8580

ttaattaaag ttacacttgt gttccttttt gttgctgcta ttttctattt aataacacct 8640

gttcatgtca tgtctaaaca tactgacttt tcaagtgaaa tcataggata caaggctatt 8700

gatggtggtg tcactcgtga catagcatct acagatactt gttttgctaa caaacatgct 8760

gattttgaca catggtttag ccagcgtggt ggtagttata ctaatgacaa agcttgccca 8820

ttgattgctg cagtcataac aagagaagtg ggttttgtcg tgcctggttt gcctggcacg 8880

atattacgca caactaatgg tgactttttg catttcttac ctagagtttt tagtgcagtt 8940

ggtaacatct gttacacacc atcaaaactt atagagtaca ctgactttgc aacatcagct 9000

tgtgttttgg ctgctgaatg tacaattttt aaagatgctt ctggtaagcc agtaccatat 9060

tgttatgata ccaatgtact agaaggttct gttgcttatg aaagtttacg ccctgacaca 9120

cgttatgtgc tcatggatgg ctctattatt caatttccta acacctacct tgaaggttct 9180

gttagagtgg taacaacttt tgattctgag tactgtaggc acggcacttg tgaaagatca 9240

gaagctggtg tttgtgtatc tactagtggt agatgggtac ttaacaatga ttattacaga 9300

tctttaccag gagttttctg tggtgtagat gctgtaaatt tacttactaa tatgtttaca 9360

ccactaattc aacctattgg tgctttggac atatcagcat ctatagtagc tggtggtatt 9420

gtagctatcg tagtaacatg ccttgcctac tattttatga ggtttagaag agcttttggt 9480

gaatacagtc atgtagttgc ctttaatact ttactattcc ttatgtcatt cactgtactc 9540

tgtttaacac cagtttactc attcttacct ggtgtttatt ctgttattta cttgtacttg 9600

acattttatc ttactaatga tgtttctttt ttagcacata ttcagtggat ggttatgttc 9660

acacctttag tacctttctg gataacaatt gcttatatca tttgtatttc cacaaagcat 9720

ttctattggt tctttagtaa ttacctaaag agacgtgtag tctttaatgg tgtttccttt 9780

agtacttttg aagaagctgc gctgtgcacc tttttgttaa ataaagaaat gtatctaaag 9840

ttgcgtagtg atgtgctatt acctcttacg caatataata gatacttagc tctttataat 9900

aagtacaagt attttagtgg agcaatggat acaactagct acagagaagc tgcttgttgt 9960

catctcgcaa aggctctcaa tgacttcagt aactcaggtt ctgatgttct ttaccaacca 10020

ccacaaacct ctatcacctc agctgttttg cagagtggtt ttagaaaaat ggcattccca 10080

tctggtaaag ttgagggttg tatggtacaa gtaacttgtg gtacaactac acttaacggt 10140

ctttggcttg atgacgtagt ttactgtcca agacatgtga tctgcacctc tgaagacatg 10200

cttaacccta attatgaaga tttactcatt cgtaagtcta atcataattt cttggtacag 10260

gctggtaatg ttcaactcag ggttattgga cattctatgc aaaattgtgt acttaagctt 10320

aaggttgata cagccaatcc taagacacct aagtataagt ttgttcgcat tcaaccagga 10380

cagacttttt cagtgttagc ttgttacaat ggttcaccat ctggtgttta ccaatgtgct 10440

atgaggccca atttcactat taagggttca ttccttaatg gttcatgtgg tagtgttggt 10500

tttaacatag attatgactg tgtctctttt tgttacatgc accatatgga attaccaact 10560

ggagttcatg ctggcacaga cttagaaggt aacttttatg gaccttttgt tgacaggcaa 10620

acagcacaag cagctggtac ggacacaact attacagtta atgttttagc ttggttgtac 10680

gctgctgtta taaatggaga caggtggttt ctcaatcgat ttaccacaac tcttaatgac 10740

tttaaccttg tggctatgaa gtacaattat gaacctctaa cacaagacca tgttgacata 10800

ctaggacctc tttctgctca aactggaatt gccgttttag atatgtgtgc ttcattaaaa 10860

gaattactgc aaaatggtat gaatggacgt accatattgg gtagtgcttt attagaagat 10920

gaatttacac cttttgatgt tgttagacaa tgctcaggtg ttactttcca aagtgcagtg 10980

aaaagaacaa tcaagggtac acaccactgg ttgttactca caattttgac ttcactttta 11040

gttttagtcc agagtactca atggtctttg ttcttttttt tgtatgaaaa tgccttttta 11100

ccttttgcta tgggtattat tgctatgtct gcttttgcaa tgatgtttgt caaacataag 11160

catgcatttc tctgtttgtt tttgttacct tctcttgcca ctgtagctta ttttaatatg 11220

gtctatatgc ctgctagttg ggtgatgcgt attatgacat ggttggatat ggttgatact 11280

agtttgtctg gttttaagct aaaagactgt gttatgtatg catcagctgt agtgttacta 11340

atccttatga cagcaagaac tgtgtatgat gatggtgcta ggagagtgtg gacacttatg 11400

aatgtcttga cactcgttta taaagtttat tatggtaatg ctttagatca agccatttcc 11460

atgtgggctc ttataatctc tgttacttct aactactcag gtgtagttac aactgtcatg 11520

tttttggcca gaggtattgt ttttatgtgt gttgagtatt gccctatttt cttcataact 11580

ggtaatacac ttcagtgtat aatgctagtt tattgtttct taggctattt ttgtacttgt 11640

tactttggcc tcttttgttt actcaaccgc tactttagac tgactcttgg tgtttatgat 11700

tacttagttt ctacacagga gtttagatat atgaattcac agggactact cccacccaag 11760

aatagcatag atgccttcaa actcaacatt aaattgttgg gtgttggtgg caaaccttgt 11820

atcaaagtag ccactgtaca gtctaaaatg tcagatgtaa agtgcacatc agtagtctta 11880

ctctcagttt tgcaacaact cagagtagaa tcatcatcta aattgtgggc tcaatgtgtc 11940

cagttacaca atgacattct cttagctaaa gatactactg aagcctttga aaaaatggtt 12000

tcactacttt ctgttttgct ttccatgcag ggtgctgtag acataaacaa gctttgtgaa 12060

gaaatgctgg acaacagggc aaccttacaa gctatagcct cagagtttag ttcccttcca 12120

tcatatgcag cttttgctac tgctcaagaa gcttatgagc aggctgttgc taatggtgat 12180

tctgaagttg ttcttaaaaa gttgaagaag tctttgaatg tggctaaatc tgaatttgac 12240

cgtgatgcag ccatgcaacg taagttggaa aagatggctg atcaagctat gacccaaatg 12300

tataaacagg ctagatctga ggacaagagg gcaaaagtta ctagtgctat gcagacaatg 12360

cttttcacta tgcttagaaa gttggataat gatgcactca acaacattat caacaatgca 12420

agagatggtt gtgttccctt gaacataata cctcttacaa cagcagccaa actaatggtt 12480

gtcataccag actataacac atataaaaat acgtgtgatg gtacaacatt tacttatgca 12540

tcagcattgt gggaaatcca acaggttgta gatgcagata gtaaaattgt tcaacttagt 12600

gaaattagta tggacaattc acctaattta gcatggcctc ttattgtaac agctttaagg 12660

gccaattctg ctgtcaaatt acagaataat gagcttagtc ctgttgcact acgacagatg 12720

tcttgtgctg ccggtactac acaaactgct tgcactgatg acaatgcgtt agcttactac 12780

aacacaacaa agggaggtag gtttgtactt gcactgttat ccgatttaca ggatttgaaa 12840

tgggctagat tccctaagag tgatggaact ggtactatct atacagaact ggaaccacct 12900

tgtaggtttg ttacagacac acctaaaggt cctaaagtga agtatttata ctttattaaa 12960

ggattaaaca acctaaatag aggtatggta cttggtagtt tagctgccac agtacgtcta 13020

caagctggta atgcaacaga agtgcctgcc aattcaactg tattatcttt ctgtgctttt 13080

gctgtagatg ctgctaaagc ttacaaagat tatctagcta gtgggggaca accaatcact 13140

aattgtgtta agatgttgtg tacacacact ggtactggtc aggcaataac agttacaccg 13200

gaagccaata tggatcaaga atcctttggt ggtgcatcgt gttgtctgta ctgccgttgc 13260

cacatagatc atccaaatcc taaaggattt tgtgacttaa aaggtaagta tgtacaaata 13320

cctacaactt gtgctaatga ccctgtgggt tttacactta aaaacacagt ctgtaccgtc 13380

tgcggtatgt ggaaaggtta tggctgtagt tgtgatcaac tccgcgaacc catgcttcag 13440

tcagctgatg cacaatcgtt tttaaacggg tttgcggtgt aagtgcagcc cgtcttacac 13500

cgtgcggcac aggcactagt actgatgtcg tatacagggc ttttgacatc tacaatgata 13560

aagtagctgg ttttgctaaa ttcctaaaaa ctaattgttg tcgcttccaa gaaaaggacg 13620

aagatgacaa tttaattgat tcttactttg tagttaagag acacactttc tctaactacc 13680

aacatgaaga aacaatttat aatttactta aggattgtcc agctgttgct aaacatgact 13740

tctttaagtt tagaatagac ggtgacatgg taccacatat atcacgtcaa cgtcttacta 13800

aatacacaat ggcagacctc gtctatgctt taaggcattt tgatgaaggt aattgtgaca 13860

cattaaaaga aatacttgtc acatacaatt gttgtgatga tgattatttc aataaaaagg 13920

actggtatga ttttgtagaa aacccagata tattacgcgt atacgccaac ttaggtgaac 13980

gtgtacgcca agctttgtta aaaacagtac aattctgtga tgccatgcga aatgctggta 14040

ttgttggtgt actgacatta gataatcaag atctcaatgg taactggtat gatttcggtg 14100

atttcataca aaccacgcca ggtagtggag ttcctgttgt agattcttat tattcattgt 14160

taatgcctat attaaccttg accagggctt taactgcaga gtcacatgtt gacactgact 14220

taacaaagcc ttacattaag tgggatttgt taaaatatga cttcacggaa gagaggttaa 14280

aactctttga ccgttatttt aaatattggg atcagacata ccacccaaat tgtgttaact 14340

gtttggatga cagatgcatt ctgcattgtg caaactttaa tgttttattc tctacagtgt 14400

tcccacctac aagttttgga ccactagtga gaaaaatatt tgttgatggt gttccatttg 14460

tagtttcaac tggataccac ttcagagagc taggtgttgt acataatcag gatgtaaact 14520

tacatagctc tagacttagt tttaaggaat tacttgtgta tgctgctgac cctgctatgc 14580

acgctgcttc tggtaatcta ttactagata aacgcactac gtgcttttca gtagctgcac 14640

ttactaacaa tgttgctttt caaactgtca aacccggtaa ttttaacaaa gacttctatg 14700

actttgctgt gtctaagggt ttctttaagg aaggaagttc tgttgaatta aaacacttct 14760

tctttgctca ggatggtaat gctgctatca gcgattatga ctactatcgt tataatctac 14820

caacaatgtg tgatatcaga caactactat ttgtagttga agttgttgat aagtactttg 14880

attgttacga tggtggctgt attaatgcta accaagtcat cgtcaacaac ctagacaaat 14940

cagctggttt tccatttaat aaatggggta aggctagact ttattatgat tcaatgagtt 15000

atgaggatca agatgcactt ttcgcatata caaaacgtaa tgtcatccct actataactc 15060

aaatgaatct taagtatgcc attagtgcaa agaatagagc tcgcaccgta gctggtgtct 15120

ctatctgtag tactatgacc aatagacagt ttcatcaaaa attattgaaa tcaatagccg 15180

ccactagagg agctactgta gtaattggaa caagcaaatt ctatggtggt tggcacaaca 15240

tgttaaaaac tgtttatagt gatgtagaaa accctcacct tatgggttgg gattatccta 15300

aatgtgatag agccatgcct aacatgctta gaattatggc ctcacttgtt cttgctcgca 15360

aacatacaac gtgttgtagc ttgtcacacc gtttctatag attagctaat gagtgtgctc 15420

aagtattgag tgaaatggtc atgtgtggcg gttcactata tgttaaacca ggtggaacct 15480

catcaggaga tgccacaact gcttatgcta atagtgtttt taacatttgt caagctgtca 15540

cggccaatgt taatgcactt ttatctactg atggtaacaa aattgccgat aagtatgtcc 15600

gcaatttaca acacagactt tatgagtgtc tctatagaaa tagagatgtt gacacagact 15660

ttgtgaatga gttttacgca tatttgcgta aacatttctc aatgatgata ctctctgacg 15720

atgctgttgt gtgtttcaat agcacttatg catctcaagg tctagtggct agcataaaga 15780

actttaagtc agttctttat tatcaaaaca atgtttttat gtctgaagca aaatgttgga 15840

ctgagactga ccttactaaa ggacctcatg aattttgctc tcaacataca atgctagtta 15900

aacagggtga tgattatgtg taccttcctt acccagatcc atcaagaatc ctaggggccg 15960

gctgttttgt agatgatatc gtaaaaacag atggtacact tatgattgaa cggttcgtgt 16020

ctttagctat agatgcttac ccacttacta aacatcctaa tcaggagtat gctgatgtct 16080

ttcatttgta cttacaatac ataagaaagc tacatgatga gttaacagga cacatgttag 16140

acatgtattc tgttatgctt actaatgata acacttcaag gtattgggaa cctgagtttt 16200

atgaggctat gtacacaccg catacagtct tacaggctgt tggggcttgt gttctttgca 16260

attcacagac ttcattaaga tgtggtgctt gcatacgtag accattctta tgttgtaaat 16320

gctgttacga ccatgtcata tcaacatcac ataaattagt cttgtctgtt aatccgtatg 16380

tttgcaatgc tccaggttgt gatgtcacag atgtgactca actttactta ggaggtatga 16440

gctattattg taaatcacat aaaccaccca ttagttttcc attgtgtgct aatggacaag 16500

tttttggttt atataaaaat acatgtgttg gtagcgataa tgttactgac tttaatgcaa 16560

ttgcaacatg tgactggaca aatgctggtg attacatttt agctaacacc tgtactgaaa 16620

gactcaagct ttttgcagca gaaacgctca aagctactga ggagacattt aaactgtctt 16680

atggtattgc tactgtacgt gaagtgctgt ctgacagaga attacatctt tcatgggaag 16740

ttggtaaacc tagaccacca cttaaccgaa attatgtctt tactggttat cgtgtaacta 16800

aaaacagtaa agtacaaata ggagagtaca cctttgaaaa aggtgactat ggtgatgctg 16860

ttgtttaccg aggtacaaca acttacaaat taaatgttgg tgattatttt gtgctgacat 16920

cacatacagt aatgccatta agtgcaccta cactagtgcc acaagagcac tatgttagaa 16980

ttactggctt atacccaaca ctcaatatct cagatgagtt ttctagcaat gttgcaaatt 17040

atcaaaaggt tggtatgcaa aagtattcta cactccaggg accacctggt actggtaaga 17100

gtcattttgc tattggccta gctctctact acccttctgc tcgcatagtg tatacagctt 17160

gctctcatgc cgctgttgat gcactatgtg agaaggcatt aaaatatttg cctatagata 17220

aatgtagtag aattatacct gcacgtgctc gtgtagagtg ttttgataaa ttcaaagtga 17280

attcaacatt agaacagtat gtcttttgta ctgtaaatgc attgcctgag acgacagcag 17340

atatagttgt ctttgatgaa atttcaatgg ccacaaatta tgatttgagt gttgtcaatg 17400

ccagattacg tgctaagcac tatgtgtaca ttggcgaccc tgctcaatta cctgcaccac 17460

gcacattgct aactaagggc acactagaac cagaatattt caattcagtg tgtagactta 17520

tgaaaactat aggtccagac atgttcctcg gaacttgtcg gcgttgtcct gctgaaattg 17580

ttgacactgt gagtgctttg gtttatgata ataagcttaa agcacataaa gacaaatcag 17640

ctcaatgctt taaaatgttt tataagggtg ttatcacgca tgatgtttca tctgcaatta 17700

acaggccaca aataggcgtg gtaagagaat tccttacacg taaccctgct tggagaaaag 17760

ctgtctttat ttcaccttat aattcacaga atgctgtagc ctcaaagatt ttgggactac 17820

caactcaaac tgttgattca tcacagggct cagaatatga ctatgtcata ttcactcaaa 17880

ccactgaaac agctcactct tgtaatgtaa acagatttaa tgttgctatt accagagcaa 17940

aagtaggcat actttgcata atgtctgata gagaccttta tgacaagttg caatttacaa 18000

gtcttgaaat tccacgtagg aatgtggcaa ctttacaagc tgaaaatgta acaggactct 18060

ttaaagattg tagtaaggta atcactgggt tacatcctac acaggcacct acacacctca 18120

gtgttgacac taaattcaaa actgaaggtt tatgtgttga catacctggc atacctaagg 18180

acatgaccta tagaagactc atctctatga tgggttttaa aatgaattat caagttaatg 18240

gttaccctaa catgtttatc acccgcgaag aagctataag acatgtacgt gcatggattg 18300

gcttcgatgt cgaggggtgt catgctacta gagaagctgt tggtaccaat ttacctttac 18360

agctaggttt ttctacaggt gttaacctag ttgctgtacc tacaggttat gttgatacac 18420

ctaataatac agatttttcc agagttagtg ctaaaccacc gcctggagat caatttaaac 18480

acctcatacc acttatgtac aaaggacttc cttggaatgt agtgcgtata aagattgtac 18540

aaatgttaag tgacacactt aaaaatctct ctgacagagt cgtatttgtc ttatgggcac 18600

atggctttga gttgacatct atgaagtatt ttgtgaaaat aggacctgag cgcacctgtt 18660

gtctatgtga tagacgtgcc acatgctttt ccactgcttc agacacttat gcctgttggc 18720

atcattctat tggatttgat tacgtctata atccgtttat gattgatgtt caacaatggg 18780

gttttacagg taacctacaa agcaaccatg atctgtattg tcaagtccat ggtaatgcac 18840

atgtagctag ttgtgatgca atcatgacta ggtgtctagc tgtccacgag tgctttgtta 18900

agcgtgttga ctggactatt gaatatccta taattggtga tgaactgaag attaatgcgg 18960

cttgtagaaa ggttcaacac atggttgtta aagctgcatt attagcagac aaattcccag 19020

ttcttcacga cattggtaac cctaaagcta ttaagtgtgt acctcaagct gatgtagaat 19080

ggaagttcta tgatgcacag ccttgtagtg acaaagctta taaaatagaa gaattattct 19140

attcttatgc cacacattct gacaaattca cagatggtgt atgcctattt tggaattgca 19200

atgtcgatag atatcctgct aattccattg tttgtagatt tgacactaga gtgctatcta 19260

accttaactt gcctggttgt gatggtggca gtttgtatgt aaataaacat gcattccaca 19320

caccagcttt tgataaaagt gcttttgtta atttaaaaca attaccattt ttctattact 19380

ctgacagtcc atgtgagtct catggaaaac aagtagtgtc agatatagat tatgtaccac 19440

taaagtctgc tacgtgtata acacgttgca atttaggtgg tgctgtctgt agacatcatg 19500

ctaatgagta cagattgtat ctcgatgctt ataacatgat gatctcagct ggctttagct 19560

tgtgggttta caaacaattt gatacttata acctctggaa cacttttaca agacttcaga 19620

gtttagaaaa tgtggctttt aatgttgtaa ataagggaca ctttgatgga caacagggtg 19680

aagtaccagt ttctatcatt aataacactg tttacacaaa agttgatggt gttgatgtag 19740

aattgtttga aaataaaaca acattacctg ttaatgtagc atttgagctt tgggctaagc 19800

gcaacattaa accagtacca gaggtgaaaa tactcaataa tttgggtgtg gacattgctg 19860

ctaatactgt gatctgggac tacaaaagag atgctccagc acatatatct actattggtg 19920

tttgttctat gactgacata gccaagaaac caactgaaac gatttgtgca ccactcactg 19980

tcttttttga tggtagagtt gatggtcaag tagacttatt tagaaatgcc cgtaatggtg 20040

ttcttattac agaaggtagt gttaaaggtt tacaaccatc tgtaggtccc aaacaagcta 20100

gtcttaatgg agtcacatta attggagaag ccgtaaaaac acagttcaat tattataaga 20160

aagttgatgg tgttgtccaa caattacctg aaacttactt tactcagagt agaaatttac 20220

aagaatttaa acccaggagt caaatggaaa ttgatttctt agaattagct atggatgaat 20280

tcattgaacg gtataaatta gaaggctatg ccttcgaaca tatcgtttat ggagatttta 20340

gtcatagtca gttaggtggt ttacatctac tgattggact agctaaacgt tttaaggaat 20400

caccttttga attagaagat tttattccta tggacagtac agttaaaaac tatttcataa 20460

cagatgcgca aacaggttca tctaagtgtg tgtgttctgt tattgattta ttacttgatg 20520

attttgttga aataataaaa tcccaagatt tatctgtagt ttctaaggtt gtcaaagtga 20580

ctattgacta tacagaaatt tcatttatgc tttggtgtaa agatggccat gtagaaacat 20640

tttacccaaa attacaatct agtcaagcgt ggcaaccggg tgttgctatg cctaatcttt 20700

acaaaatgca aagaatgcta ttagaaaagt gtgaccttca aaattatggt gatagtgcaa 20760

cattacctaa aggcataatg atgaatgtcg caaaatatac tcaactgtgt caatatttaa 20820

acacattaac attagctgta ccctataata tgagagttat acattttggt gctggttctg 20880

ataaaggagt tgcaccaggt acagctgttt taagacagtg gttgcctacg ggtacgctgc 20940

ttgtcgattc agatcttaat gactttgtct ctgatgcaga ttcaactttg attggtgatt 21000

gtgcaactgt acatacagct aataaatggg atctcattat tagtgatatg tacgacccta 21060

agactaaaaa tgttacaaaa gaaaatgact ctaaagaggg ttttttcact tacatttgtg 21120

ggtttataca acaaaagcta gctcttggag gttccgtggc tataaagata acagaacatt 21180

cttggaatgc tgatctttat aagctcatgg gacacttcgc atggtggaca gcctttgtta 21240

ctaatgtgaa tgcgtcatca tctgaagcat ttttaattgg atgtaattat cttggcaaac 21300

cacgcgaaca aatagatggt tatgtcatgc atgcaaatta catattttgg aggaatacaa 21360

atccaattca gttgtcttcc tattctttat ttgacatgag taaatttccc cttaaattaa 21420

ggggtactgc tgttatgtct ttaaaagaag gtcaaatcaa tgatatgatt ttatctcttc 21480

ttagtaaagg tagacttata attagagaaa acaacagagt tgttatttct agtgatgttc 21540

ttgttaacaa ctaaacgaac aatgtttgtt tttcttgttt tattgccact agtctctagt 21600

cagtgtgtta atcttacaac cagaactcaa ttaccccctg catacactaa ttctttcaca 21660

cgtggtgttt attaccctga caaagttttc agatcctcag ttttacattc aactcaggac 21720

ttgttcttac ctttcttttc caatgttact tggttccatg ctatacatgt ctctgggacc 21780

aatggtacta agaggtttga taaccctgtc ctaccattta atgatggtgt ttattttgct 21840

tccactgaga agtctaacat aataagaggc tggatttttg gtactacttt agattcgaag 21900

acccagtccc tacttattgt taataacgct actaatgttg ttattaaagt ctgtgaattt 21960

caattttgta atgatccatt tttgggtgtt tattaccaca aaaacaacaa aagttggatg 22020

gaaagtgagt tcagagttta ttctagtgcg aataattgca cttttgaata tgtctctcag 22080

ccttttctta tggaccttga aggaaaacag ggtaatttca aaaatcttag ggaatttgtg 22140

tttaagaata ttgatggtta ttttaaaata tattctaagc acacgcctat taatttagtg 22200

cgtgatctcc ctcagggttt ttcggcttta gaaccattgg tagatttgcc aataggtatt 22260

aacatcacta ggtttcaaac tttacttgct ttacatagaa gttatttgac tcctggtgat 22320

tcttcttcag gttggacagc tggtgctgca gcttattatg tgggttatct tcaacctagg 22380

acttttctat taaaatataa tgaaaatgga accattacag atgctgtaga ctgtgcactt 22440

gaccctctct cagaaacaaa gtgtacgttg aaatccttca ctgtagaaaa aggaatctat 22500

caaacttcta actttagagt ccaaccaaca gaatctattg ttagatttcc taatattaca 22560

aacttgtgcc cttttggtga agtttttaac gccaccagat ttgcatctgt ttatgcttgg 22620

aacaggaaga gaatcagcaa ctgtgttgct gattattctg tcctatataa ttccgcatca 22680

ttttccactt ttaagtgtta tggagtgtct cctactaaat taaatgatct ctgctttact 22740

aatgtctatg cagattcatt tgtaattaga ggtgatgaag tcagacaaat cgctccaggg 22800

caaactggaa agattgctga ttataattat aaattaccag atgattttac aggctgcgtt 22860

atagcttgga attctaacaa tcttgattct aaggttggtg gtaattataa ttacctgtat 22920

agattgttta ggaagtctaa tctcaaacct tttgagagag atatttcaac tgaaatctat 22980

caggccggta gcacaccttg taatggtgtt gaaggtttta attgttactt tcctttacaa 23040

tcatatggtt tccaacccac taatggtgtt ggttaccaac catacagagt agtagtactt 23100

tcttttgaac ttctacatgc accagcaact gtttgtggac ctaaaaagtc tactaatttg 23160

gttaaaaaca aatgtgtcaa tttcaacttc aatggtttaa caggcacagg tgttcttact 23220

gagtctaaca aaaagtttct gcctttccaa caatttggca gagacattgc tgacactact 23280

gatgctgtcc gtgatccaca gacacttgag attcttgaca ttacaccatg ttcttttggt 23340

ggtgtcagtg ttataacacc aggaacaaat acttctaacc aggttgctgt tctttatcag 23400

gatgttaact gcacagaagt ccctgttgct attcatgcag atcaacttac tcctacttgg 23460

cgtgtttatt ctacaggttc taatgttttt caaacacgtg caggctgttt aataggggct 23520

gaacatgtca acaactcata tgagtgtgac atacccattg gtgcaggtat atgcgctagt 23580

tatcagactc agactaattc tcctcggcgg gcacgtagtg tagctagtca atccatcatt 23640

gcctacacta tgtcacttgg tgcagaaaat tcagttgctt actctaataa ctctattgcc 23700

atacccacaa attttactat tagtgttacc acagaaattc taccagtgtc tatgaccaag 23760

acatcagtag attgtacaat gtacatttgt ggtgattcaa ctgaatgcag caatcttttg 23820

ttgcaatatg gcagtttttg tacacaatta aaccgtgctt taactggaat agctgttgaa 23880

caagacaaaa acacccaaga agtttttgca caagtcaaac aaatttacaa aacaccacca 23940

attaaagatt ttggtggttt taatttttca caaatattac cagatccatc aaaaccaagc 24000

aagaggtcat ttattgaaga tctacttttc aacaaagtga cacttgcaga tgctggcttc 24060

atcaaacaat atggtgattg ccttggtgat attgctgcta gagacctcat ttgtgcacaa 24120

aagtttaacg gccttactgt tttgccacct ttgctcacag atgaaatgat tgctcaatac 24180

acttctgcac tgttagcggg tacaatcact tctggttgga cctttggtgc aggtgctgca 24240

ttacaaatac catttgctat gcaaatggct tataggttta atggtattgg agttacacag 24300

aatgttctct atgagaacca aaaattgatt gccaaccaat ttaatagtgc tattggcaaa 24360

attcaagact cactttcttc cacagcaagt gcacttggaa aacttcaaga tgtggtcaac 24420

caaaatgcac aagctttaaa cacgcttgtt aaacaactta gctccaattt tggtgcaatt 24480

tcaagtgttt taaatgatat cctttcacgt cttgacaaag ttgaggctga agtgcaaatt 24540

gataggttga tcacaggcag acttcaaagt ttgcagacat atgtgactca acaattaatt 24600

agagctgcag aaatcagagc ttctgctaat cttgctgcta ctaaaatgtc agagtgtgta 24660

cttggacaat caaaaagagt tgatttttgt ggaaagggct atcatcttat gtccttccct 24720

cagtcagcac ctcatggtgt agtcttcttg catgtgactt atgtccctgc acaagaaaag 24780

aacttcacaa ctgctcctgc catttgtcat gatggaaaag cacactttcc tcgtgaaggt 24840

gtctttgttt caaatggcac acactggttt gtaacacaaa ggaattttta tgaaccacaa 24900

atcattacta cagacaacac atttgtgtct ggtaactgtg atgttgtaat aggaattgtc 24960

aacaacacag tttatgatcc tttgcaacct gaattagact cattcaagga ggagttagat 25020

aaatatttta agaatcatac atcaccagat gttgatttag gtgacatctc tggcattaat 25080

gcttcagttg taaacattca aaaagaaatt gaccgcctca atgaggttgc caagaattta 25140

aatgaatctc tcatcgatct ccaagaactt ggaaagtatg agcagtatat aaaatggcca 25200

tggtacattt ggctaggttt tatagctggc ttgattgcca tagtaatggt gacaattatg 25260

ctttgctgta tgaccagttg ctgtagttgt ctcaagggct gttgttcttg tggatcctgc 25320

tgcaaatttg atgaagacga ctctgagcca gtgctcaaag gagtcaaatt acattacaca 25380

taaacgaact tatggatttg tttatgagaa tcttcacaat tggaactgta actttgaagc 25440

aaggtgaaat caaggatgct actccttcag attttgttcg cgctactgca acgataccga 25500

tacaagcctc actccctttc ggatggctta ttgttggcgt tgcacttctt gctgtttttc 25560

agagcgcttc caaaatcata accctcaaaa agagatggca actagcactc tccaagggtg 25620

ttcactttgt ttgcaacttg ctgttgttgt ttgtaacagt ttactcacac cttttgctcg 25680

ttgctgctgg ccttgaagcc ccttttctct atctttatgc tttagtctac ttcttgcaga 25740

gtataaactt tgtaagaata ataatgaggc tttggctttg ctggaaatgc cgttccaaaa 25800

acccattact ttatgatgcc aactattttc tttgctggca tactaattgt tacgactatt 25860

gtatacctta caatagtgta acttcttcaa ttgtcattac ttcaggtgat ggcacaacaa 25920

gtcctatttc tgaacatgac taccagattg gtggttatac tgaaaaatgg gaatctggag 25980

taaaagactg tgttgtatta cacagttact tcacttcaga ctattaccag ctgtactcaa 26040

ctcaattgag tacagacact ggtgttgaac atgttacctt cttcatctac aataaaattg 26100

ttgatgagcc tgaagaacat gtccaaattc acacaatcga cggttcatcc ggagttgtta 26160

atccagtaat ggaaccaatt tatgatgaac cgacgacgac tactagcgtg cctttgtaag 26220

cacaagctga tgagtacgaa cttatgtact cattcgtttc ggaagagaca ggtacgttaa 26280

tagttaatag cgtacttctt tttcttgctt tcgtggtatt cttgctagtt acactagcca 26340

tccttactgc gcttcgattg tgtgcgtact gctgcaatat tgttaacgtg agtcttgtaa 26400

aaccttcttt ttacgtttac tctcgtgtta aaaatctgaa ttcttctaga gttcctgatc 26460

ttctggtcta aacgaactaa atattatatt agtttttctg tttggaactt taattttagc 26520

catggcagat tccaacggta ctattaccgt tgaagagctt aaaaagctcc ttgaacaatg 26580

gaacctagta ataggtttcc tattccttac atggatttgt cttctacaat ttgcctatgc 26640

caacaggaat aggtttttgt atataattaa gttaattttc ctctggctgt tatggccagt 26700

aactttagct tgttttgtgc ttgctgctgt ttacagaata aattggatca ccggtggaat 26760

tgctatcgca atggcttgtc ttgtaggctt gatgtggctc agctacttca ttgcttcttt 26820

cagactgttt gcgcgtacgc gttccatgtg gtcattcaat ccagaaacta acattcttct 26880

caacgtgcca ctccatggca ctattctgac cagaccgctt ctagaaagtg aactcgtaat 26940

cggagctgtg atccttcgtg gacatcttcg tattgctgga caccatctag gacgctgtga 27000

catcaaggac ctgcctaaag aaatcactgt tgctacatca cgaacgcttt cttattacaa 27060

attgggagct tcgcagcgtg tagcaggtga ctcaggtttt gctgcataca gtcgctacag 27120

gattggcaac tataaattaa acacagacca ttccagtagc agtgacaata ttgctttgct 27180

tgtacagtaa gtgacaacag atgtttcatc tcgttgactt tcaggttact atagcagaga 27240

tattactaat tattatgagg acttttaaag tttccatttg gaatcttgat tacatcataa 27300

acctcataat taaaaattta tctaagtcac taactgagaa taaatattct caattagatg 27360

aagagcaacc aatggagatt gattaaacga acatgaaaat tattcttttc ttggcactga 27420

taacactcgc tacttgtgag ctttatcact accaagagtg tgttagaggt acaacagtac 27480

ttttaaaaga accttgctct tctggaacat acgagggcaa ttcaccattt catcctctag 27540

ctgataacaa atttgcactg acttgcttta gcactcaatt tgcttttgct tgtcctgacg 27600

gcgtaaaaca cgtctatcag ttacgtgcca gatcagtttc acctaaactg ttcatcagac 27660

aagaggaagt tcaagaactt tactctccaa tttttcttat tgttgcggca atagtgttta 27720

taacactttg cttcacactc aaaagaaaga cagaatgatt gaactttcat taattgactt 27780

ctatttgtgc tttttagcct ttctgctatt ccttgtttta attatgctta ttatcttttg 27840

gttctcactt gaactgcaag atcataatga aacttgtcac gcctaaacga acatgaaatt 27900

tcttgttttc ttaggaatca tcacaactgt agctgcattt caccaagaat gtagtttaca 27960

gtcatgtact caacatcaac catatgtagt tgatgacccg tgtcctattc acttctattc 28020

taaatggtat attagagtag gagctagaaa atcagcacct ttaattgaat tgtgcgtgga 28080

tgaggctggt tctaaatcac ccattcagta catcgatatc ggtaattata cagtttcctg 28140

tttacctttt acaattaatt gccaggaacc taaattgggt agtcttgtag tgcgttgttc 28200

gttctatgaa gactttttag agtatcatga cgttcgtgtt gttttagatt tcatctaaac 28260

gaacaaacta aaatgtctga taatggaccc caaaatcagc gaaatgcacc ccgcattacg 28320

tttggtggac cctcagattc aactggcagt aaccagaatg gagaacgcag tggggcgcga 28380

tcaaaacaac gtcggcccca aggtttaccc aataatactg cgtcttggtt caccgctctc 28440

actcaacatg gcaaggaaga ccttaaattc cctcgaggac aaggcgttcc aattaacacc 28500

aatagcagtc cagatgacca aattggctac taccgaagag ctaccagacg aattcgtggt 28560

ggtgacggta aaatgaaaga tctcagtcca agatggtatt tctactacct aggaactggg 28620

ccagaagctg gacttcccta tggtgctaac aaagacggca tcatatgggt tgcaactgag 28680

ggagccttga atacaccaaa agatcacatt ggcacccgca atcctgctaa caatgctgca 28740

atcgtgctac aacttcctca aggaacaaca ttgccaaaag gcttctacgc agaagggagc 28800

agaggcggca gtcaagcctc ttctcgttcc tcatcacgta gtcgcaacag ttcaagaaat 28860

tcaactccag gcagcagtag gggaacttct cctgctagaa tggctggcaa tggcggtgat 28920

gctgctcttg ctttgctgct gcttgacaga ttgaaccagc ttgagagcaa aatgtctggt 28980

aaaggccaac aacaacaagg ccaaactgtc actaagaaat ctgctgctga ggcttctaag 29040

aagcctcggc aaaaacgtac tgccactaaa gcatacaatg taacacaagc tttcggcaga 29100

cgtggtccag aacaaaccca aggaaatttt ggggaccagg aactaatcag acaaggaact 29160

gattacaaac attggccgca aattgcacaa tttgccccca gcgcttcagc gttcttcgga 29220

atgtcgcgca ttggcatgga agtcacacct tcgggaacgt ggttgaccta cacaggtgcc 29280

atcaaattgg atgacaaaga tccaaatttc aaagatcaag tcattttgct gaataagcat 29340

attgacgcat acaaaacatt cccaccaaca gagcctaaaa aggacaaaaa gaagaaggct 29400

gatgaaactc aagccttacc gcagagacag aagaaacagc aaactgtgac tcttcttcct 29460

gctgcagatt tggatgattt ctccaaacaa ttgcaacaat ccatgagcag tgctgactca 29520

actcaggcct aaactcatgc agaccacaca aggcagatgg gctatataaa cgttttcgct 29580

tttccgttta cgatatatag tctactcttg tgcagaatga attctcgtaa ctacatagca 29640

caagtagatg tagttaactt taatctcaca tagcaatctt taatcagtgt gtaacattag 29700

ggaggacttg aaagagccac cacattttca ccgaggccac gcggagtacg atcgagtgta 29760

cagtgaacaa tgctagggag agctgcctat atggaagagc cctaatgtgt aaaattaatt 29820

ttagtagtgc tatccccatg tgattttaat agcttcttag gagaatgaca aaaaaaaaaa 29880

a 29881

<210> 228

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 228

gmsumsgmug aucauugugg ugaamsamsc m 31

<210> 229

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 229

umsumscmac cacaaugauc acacmsumsu m 31

<210> 230

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 230

umsumsgmuc accugugcaa aggamsamsa m 31

<210> 231

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 231

umscmscmuu ugcacaggug acaamsumsu m 31

<210> 232

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 232

amsgmsgmga uuguacagaa agugmsumsg m 31

<210> 233

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 233

cmsamscmuu ucuguacaau cccumsumsu m 31

<210> 234

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 234

cmsumscmgg uacagaagua aaugmsamsg m 31

<210> 235

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 235

cmsamsumuu acuucuguac cgagmsumsu m 31

<210> 236

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 236

gmsgmsumga uugugaagaa gaagmsamsg m 31

<210> 237

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 237

cmsumsumcu ucuucacaau caccmsumsu m 31

<210> 238

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 238

gmsamsamga gcaagaagaa gauumsgmsg m 31

<210> 239

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 239

amsamsumcu ucuucuugcu cuucmsumsu m 31

<210> 240

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 240

umsgmsgmug augugguggc uauumsgmsa m 31

<210> 241

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 241

amsamsumag ccaccacauc accamsumsu m 31

<210> 242

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 242

amscmscmaa auaccuggug uauamscmsg m 31

<210> 243

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 243

umsamsumac accagguauu uggumsumsu m 31

<210> 244

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 244

amsumsamcc ugguguauac guugmsumsc m 31

<210> 245

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 245

cmsamsamcg uauacaccag guaumsumsu m 31

<210> 246

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 246

cmsumsamcc gaaguuguag gagamscmsa m 31

<210> 247

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 247

umscmsumcc uacaacuucg guagmsumsu m 31

<210> 248

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 248

gmsgmsgmca cacuagaacc agaamsumsa m 31

<210> 249

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 249

umsumscmug guucuagugu gcccmsumsu m 31

<210> 250

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 250

gmsgmsgmug uuaucacgca ugaumsgmsu m 31

<210> 251

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 251

amsumscmau gcgugauaac acccmsumsu m 31

<210> 252

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 252

umsamsgmgc gugguaagag aauumscmsc m 31

<210> 253

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 253

amsamsumuc ucuuaccacg ccuamsumsu m 31

<210> 254

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 254

amsumsgmcc cguaauggug uucumsumsa m 31

<210> 255

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 255

amsgmsamac accauuacgg gcaumsumsu m 31

<210> 256

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 256

gmscmsumag ucuuaaugga gucamscmsa m 31

<210> 257

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 257

umsgmsamcu ccauuaagac uagcmsumsu m 31

<210> 258

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 258

gmsumsumga ugguguuguc caacmsamsa m 31

<210> 259

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 259

gmsumsumgg acaacaccau caacmsumsu m 31

<210> 260

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 260

umsumscmuu ucacacgugg uguumsumsa m 31

<210> 261

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 261

amsamscmac cacgugugaa agaamsumsu m 31

<210> 262

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 262

cmscmscmug uccuaccauu uaaumsgmsa m 31

<210> 263

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 263

amsumsumaa augguaggac agggmsumsu m 31

<210> 264

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 264

amsamsgmac ccagucccua cuuamsumsu m 31

<210> 265

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 265

amscmsamau aaguagggac ugggmsumsc m 31

<210> 266

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 266

amsamsgmuu ggauggaaag ugagmsumsu m 31

<210> 267

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 267

umsgmsamac ucacuuucca uccamsamsc m 31

<210> 268

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 268

amsamsgmug uacguugaaa uccumsumsc m 31

<210> 269

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 269

gmsumsgmaa ggauuucaac guacmsamsc m 31

<210> 270

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 270

amsamscmgc caccagauuu gcaumscmsu m 31

<210> 271

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 271

amscmsamga ugcaaaucug guggmscmsg m 31

<210> 272

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 272

amsamscmag gaagagaauc agcamsamsc m 31

<210> 273

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 273

cmsamsgmuu gcugauucuc uuccmsumsg m 31

<210> 274

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 274

amsamscmcc acuaauggug uuggmsumsu m 31

<210> 275

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 275

gmsumsamac caacaccauu agugmsgmsg m 31

<210> 276

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 276

amsamscmuu cuacaugcac cagcmsamsa m 31

<210> 277

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 277

amsgmsumug cuggugcaug uagamsamsg m 31

<210> 278

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 278

amsamscmca gguugcuguu cuuumsamsu m 31

<210> 279

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 279

umsgmsamua aagaacagca accumsgmsg m 31

<210> 280

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 280

amsamscmac gugcaggcug uuuamsamsu m 31

<210> 281

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 281

cmsumsamuu aaacagccug cacgmsumsg m 31

<210> 282

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 282

cmsumscmua uugccauacc cacamsamsa m 31

<210> 283

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 283

umsgmsumgg guauggcaau agagmsumsu m 31

<210> 284

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 284

amsamsumcc aucauugccu acacmsumsa m 31

<210> 285

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 285

cmsamsumag uguaggcaau gaugmsgmsa m 31

<210> 286

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 286

amsamsamcc gugcuuuaac uggamsamsu m 31

<210> 287

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 287

cmsumsamuu ccaguuaaag cacgmsgmsu m 31

<210> 288

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 288

amsamscmug gaauagcugu ugaamscmsa m 31

<210> 289

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 289

cmsumsumgu ucaacagcua uuccmsamsg m 31

<210> 290

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 290

amsamsumca cuucugguug gaccmsumsu m 31

<210> 291

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 291

cmsamsamag guccaaccag aagumsgmsa m 31

<210> 292

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 292

amsamsamgu ugaggcugaa gugcmsamsa m 31

<210> 293

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 293

amsumsumug cacuucagcc ucaamscmsu m 31

<210> 294

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 294

amsamsumca gagcuucugc uaaumscmsu m 31

<210> 295

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 295

cmsamsamga uuagcagaag cucumsgmsa m 31

<210> 296

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 296

amsamsumgg cacacacugg uuugmsumsa m 31

<210> 297

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 297

gmsumsumac aaaccagugu gugcmscmsa m 31

<210> 298

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 298

amsamsumag gaauugucaa caacmsamsc m 31

<210> 299

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 299

cmsumsgmug uuguugacaa uuccmsumsa m 31

<210> 300

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 300

amsamsumcu cucaucgauc uccamsamsg m 31

<210> 301

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 301

umsumscmuu ggagaucgau gagamsgmsa m 31

<210> 302

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 302

gmsamsgmac agguacguua auagmsumsu m 31

<210> 303

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 303

cmsumsamuu aacguaccug ucucmsumsu m 31

<210> 304

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 304

gmsumsumac acuagccauc cuuamscmsu m 31

<210> 305

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 305

umsamsamgg auggcuagug uaacmsumsu m 31

<210> 306

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 306

umscmscmuu acugcgcuuc gauumsgmsu m 31

<210> 307

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 307

amsamsumcg aagcgcagua aggamsumsu m 31

<210> 308

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 308

cmsumsgmcu gcaauauugu uaacmsgmsu m 31

<210> 309

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 309

gmsumsumaa caauauugca gcagmsumsu m 31

<210> 310

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 310

umsumsgmcu aucgcaaugg cuugmsumsc m 31

<210> 311

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 311

cmsamsamgc cauugcgaua gcaamsumsu m 31

<210> 312

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 312

cmsgmsgmua cuauuaccgu ugaamsgmsa m 31

<210> 313

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 313

umsumscmaa cgguaauagu accgmsumsu m 31

<210> 314

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 314

amsgmscmuc cuugaacaau ggaamscmsc m 31

<210> 315

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 315

umsumscmca uuguucaagg agcumsumsu m 31

<210> 316

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 316

amsumsumgg aucaccggug gaaumsumsg m 31

<210> 317

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 317

amsumsumcc accggugauc caaumsumsu m 31

<210> 318

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 318

gmsgmsamcc ugccuaaaga aaucmsamsc m 31

<210> 319

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 319

gmsamsumuu cuuuaggcag guccmsumsu m 31

<210> 320

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 320

umscmsamcu guugcuacau cacgmsamsa m 31

<210> 321

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 321

cmsgmsumga uguagcaaca gugamsumsu m 31

<210> 322

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 322

umsgmsgmaa ccuaguaaua gguumsumsc m 31

<210> 323

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 323

amsamscmcu auuacuaggu uccamsumsu m 31

<210> 324

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 324

umsumsgmga ucaccggugg aauumsgmsc m 31

<210> 325

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 325

amsamsumuc caccggugau ccaamsumsu m 31

<210> 326

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 326

cmsumscmgu aaucggagcu gugamsumsc m 31

<210> 327

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 327

umscmsamca gcuccgauua cgagmsumsu m 31

<210> 328

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 328

cmscmsumag uaauagguuu ccuamsumsu m 31

<210> 329

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 329

umsamsgmga aaccuauuac uaggmsumsu m 31

<210> 330

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 330

cmsamsumgg caaggaagac cuuamsamsa m 31

<210> 331

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 331

umsamsamgg ucuuccuugc caugmsumsu m 31

<210> 332

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 332

gmsgmscmgu uccaauuaac accamsamsu m 31

<210> 333

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 333

umsgmsgmug uuaauuggaa cgccmsumsu m 31

<210> 334

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 334

cmsamscmca auagcagucc agaumsgmsa m 31

<210> 335

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 335

amsumscmug gacugcuauu ggugmsumsu m 31

<210> 336

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 336

umsamsgmca guccagauga ccaamsamsu m 31

<210> 337

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 337

umsumsgmgu caucuggacu gcuamsumsu m 31

<210> 338

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 338

umsumsgmgc uacuaccgaa gagcmsumsa m 31

<210> 339

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 339

gmscmsumcu ucgguaguag ccaamsumsu m 31

<210> 340

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 340

umscmscmug cuaacaaugc ugcamsamsu m 31

<210> 341

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 341

umsgmscmag cauuguuagc aggamsumsu m 31

<210> 342

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 342

umsgmscmug caaucgugcu acaamscmsu m 31

<210> 343

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 343

umsumsgmua gcacgauugc agcamsumsu m 31

<210> 344

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 344

umscmsgmug cuacaacuuc cucamsamsg m 31

<210> 345

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 345

umsgmsamgg aaguuguagc acgamsumsu m 31

<210> 346

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 346

cmsumsumcc ucaaggaaca acaumsumsg m 31

<210> 347

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 347

amsumsgmuu guuccuugag gaagmsumsu m 31

<210> 348

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 348

amsumsgmuc ugguaaaggc caacmsamsa m 31

<210> 349

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 349

gmsumsumgg ccuuuaccag acaumsumsu m 31

<210> 350

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 350

cmsamsamgg ccaaacuguc acuamsamsg m 31

<210> 351

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 351

umsamsgmug acaguuuggc cuugmsumsu m 31

<210> 352

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 352

amsumscmug cugcugaggc uucumsamsa m 31

<210> 353

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 353

amsgmsamag ccucagcagc agaumsumsu m 31

<210> 354

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 354

amscmsgmua cugccacuaa agcamsumsa m 31

<210> 355

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 355

umsgmscmuu uaguggcagu acgumsumsu m 31

<210> 356

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 356

amsgmscmau acaauguaac acaamsgmsc m 31

<210> 357

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 357

umsumsgmug uuacauugua ugcumsumsu m 31

<210> 358

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 358

gmsgmscmug augaaacuca agccmsumsu m 31

<210> 359

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 359

gmsgmscmuu gaguuucauc agccmsumsu m 31

<210> 360

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 360

amscmsamgc aaacugugac ucuumscmsu m 31

<210> 361

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 361

amsamsgmag ucacaguuug cugumsumsu m 31

<210> 362

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 362

umscmsamcc cauucaguac aucgmsamsu m 31

<210> 363

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 363

cmsgmsamug uacugaaugg gugamsumsu m 31

<210> 364

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 364

umsumsgmcc aggaaccuaa auugmsgmsg m 31

<210> 365

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 365

cmsamsamuu uagguuccug gcaamsumsu m 31

<210> 366

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 366

cmscmsumaa auuggguagu cuugmsumsa m 31

<210> 367

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 367

cmsamsamga cuacccaauu uaggmsumsu m 31

<210> 368

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 368

cmsamsumca accauaugua guugmsamsu m 31

<210> 369

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 369

cmsamsamcu acauaugguu gaugmsumsu m 31

<210> 370

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 370

cmscmscmgu guccuauuca cuucmsumsa m 31

<210> 371

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 371

gmsamsamgu gaauaggaca cgggmsumsu m 31

<210> 372

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 372

umscmsamgc accuuuaauu gaaumsumsg m 31

<210> 373

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 373

amsumsumca auuaaaggug cugamsumsu m 31

<210> 374

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 374

umscmsamca acuguagcug cauumsumsc m 31

<210> 375

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 375

amsamsumgc agcuacaguu gugamsumsu m 31

<210> 376

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 376

gmscmsumgc auuucaccaa gaaumsgmsu m 31

<210> 377

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 377

amsumsumcu uggugaaaug cagcmsumsu m 31

<210> 378

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 378

gmsumsumga ugacccgugu ccuamsumsu m 31

<210> 379

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 379

umsamsgmga cacgggucau caacmsumsu m 31

<210> 380

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 380

umsumsamga ugaagagcaa ccaamsumsg m 31

<210> 381

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 381

umsumsgmgu ugcucuucau cuaamsumsu m 31

<210> 382

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 382

amsgmsamgc aaccaaugga gauumsgmsa m 31

<210> 383

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 383

amsamsumcu ccauugguug cucumsumsu m 31

<210> 384

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 384

umscmsumcg uugacuuuca gguumsamsc m 31

<210> 385

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 385

amsamscmcu gaaagucaac gagamsumsu m 31

<210> 386

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 386

gmsgmsumua cuauagcaga gauamsumsu m 31

<210> 387

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 387

umsamsumcu cugcuauagu aaccmsumsu m 31

<210> 388

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 388

umscmsumaa gucacuaacu gagamsamsu m 31

<210> 389

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 389

umscmsumca guuagugacu uagamsumsu m 31

<210> 390

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 390

cmsamsumgg caaggaagac cuuamsamsa m 31

<210> 391

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 391

umsamsamgg ucuuccuugc caugmsumsu m 31

<210> 392

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 392

gmsgmscmgu uccaauuaac accamsamsu m 31

<210> 393

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 393

umsgmsgmug uuaauuggaa cgccmsumsu m 31

<210> 394

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 394

cmsamscmca auagcagucc agaumsgmsa m 31

<210> 395

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 395

amsumscmug gacugcuauu ggugmsumsu m 31

<210> 396

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 396

umscmsgmug cuacaacuuc cucamsamsg m 31

<210> 397

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 397

umsgmsamgg aaguuguagc acgamsumsu m 31

<210> 398

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 398

cmsumsumcc ucaaggaaca acaumsumsg m 31

<210> 399

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 399

amsumsgmuu guuccuugag gaagmsumsu m 31

<210> 400

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 400

gmscmscmuc uucucguucc ucaumscmsa m 31

<210> 401

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 401

amsumsgmag gaacgagaag aggcmsumsu m 31

<210> 402

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 402

cmsamscmuc gcuacuugug agcumsumsu m 31

<210> 403

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 403

amsgmscmuc acaaguagcg agugmsumsu m 31

<210> 404

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 404

gmsamsgmug uguuagaggu acaamscmsa m 31

<210> 405

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 405

umsumsgmua ccucuaacac acucmsumsu m 31

<210> 406

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 406

gmsamsamcc uugcucuucu ggaamscmsa m 31

<210> 407

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 407

umsumscmca gaagagcaag guucmsumsu m 31

<210> 408

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 408

cmscmsumug cucuucugga acaumsamsc m 31

<210> 409

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 409

amsumsgmuu ccagaagagc aaggmsumsu m 31

<210> 410

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 410

gmsamsgmga aguucaagaa cuuumsamsc m 31

<210> 411

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 411

amsamsamgu ucuugaacuu ccucmsumsu m 31

<210> 412

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 412

cmsumsgmac uugcuuuagc acucmsamsa m 31

<210> 413

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 413

gmsamsgmug cuaaagcaag ucagmsumsu m 31

<210> 414

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 414

gmsgmsumga aaucaaggau gcuamscmsu m 31

<210> 415

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 415

umsamsgmca uccuugauuu caccmsumsu m 31

<210> 416

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 416

gmsgmsgmug uucacuuugu uugcmsamsa m 31

<210> 417

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 417

gmscmsamaa caaagugaac acccmsumsu m 31

<210> 418

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 418

amsgmsamcu guguuguauu acacmsamsg m 31

<210> 419

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 419

gmsumsgmua auacaacaca gucumsumsu m 31

<210> 420

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 420

umsumscmac acaaucgacg guucmsamsu m 31

<210> 421

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 421

gmsamsamcc gucgauugug ugaamsumsu m 31

<210> 422

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 422

cmsumsamgc acucuccaag ggugmsumsu m 31

<210> 423

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 423

cmsamscmcc uuggagagug cuagmsumsu m 31

<210> 424

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 424

cmsumsumgc uguuguuguu uguamsamsc m 31

<210> 425

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 425

umsamscmaa acaacaacag caagmsumsu m 31

<210> 426

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 426

gmsumscmcu auuucugaac augamscmsu m 31

<210> 427

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 427

umscmsamug uucagaaaua ggacmsumsu m 31

<210> 428

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 428

amsumsumgu ugaugagccu gaagmsamsa m 31

<210> 429

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 429

cmsumsumca ggcucaucaa caaumsumsu m 31

<210> 430

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 430

cmsamsumgu ccaaauucac acaamsumsc m 31

<210> 431

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 431

umsumsgmug ugaauuugga caugmsumsu m 31

<210> 432

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 432

umscmsgmac gguucauccg gagumsumsg m 31

<210> 433

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 433

amscmsumcc ggaugaaccg ucgamsumsu m 31

<210> 434

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 434

umsumscmuc cgaacguguc acgumstmst m 31

<210> 435

<211> 31

<212> DNA/RNA

<213> Artificial Sequence (Artificial Sequence)

<400> 435

amscmsgmug acacguucgg agaamstmst m 31

Claims (3)

1. An siRNA for inhibiting the expression of a novel coronavirus gene, wherein the siRNA consists of a sense strand and an antisense strand, and the sense strand is as shown in SEQ ID NO: 57, and the antisense strand is as shown in SEQ ID NO: 58 of a nucleotide sequence

Wherein,

sense strand 5'-AAUCCAUCAUUGCCUACACUA-3' (SEQ ID NO: 57),

antisense strand 5'-CAUAGUGUAGGCAAUGAUGGA-3' (SEQ ID NO: 58);

the sense strand of the siRNA is SEQ ID NO: the nucleotides 1, 2, 3, 19, 20 and 21 of the 57 nucleotide sequence are all 2' -O-methyl ribonucleotide, and the antisense strand of the siRNA is SEQ ID NO: 58 nucleotide sequence No. 1, 2, 3,

19. The 20-and 21-nucleotides are both 2' -O-methyl ribonucleotide;

the nucleotide sequences of the sense strand and the antisense strand of the siRNA have phosphate groups between the 1 st, 2 nd and 3 rd nucleotides, 19 th,

20. The phosphate groups between the 21-nucleotides are all phosphorothioate groups;

the 5' end of the sense strand of the siRNA is subjected to cholesterol modification.

2. Use of the siRNA of claim 1 for the preparation of a medicament for the prevention and/or treatment of COVID-19.

3. A kit comprising the siRNA of claim 1, wherein said kit is functional for treating COVID-19.

Images