CN111875855B - Eugenol-containing curdlan-based antioxidant film and preparation method thereof - Google Patents
Eugenol-containing curdlan-based antioxidant film and preparation method thereof Download PDFInfo
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- CN111875855B CN111875855B CN202010787870.3A CN202010787870A CN111875855B CN 111875855 B CN111875855 B CN 111875855B CN 202010787870 A CN202010787870 A CN 202010787870A CN 111875855 B CN111875855 B CN 111875855B
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
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- C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
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- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
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Abstract
The invention relates to a eugenol-containing kuran-based antioxidant film and a preparation method thereof. The raw materials of the antioxidant membrane comprise natural polysaccharide, eugenol, tween 80 and glycerol. The preparation method comprises weighing eugenol, adding into water, adding curdlan, tween 80 and glycerol, magnetically stirring the membrane solution at room temperature, adjusting pH with lactic acid, homogenizing, pouring into polytetrafluoroethylene plate, standing in constant temperature and humidity cabinet (25 deg.C, 50% RH) for 24 hr or so, and demolding. The invention takes the available polysaccharide as the film matrix, the raw material has the characteristics of thermal gelation, degradability and innocuity, and the film forming property and the biological property are widely used in the food industry and the related fields. In addition, the prepared natural base film has good oxidation resistance, the preparation process is simple and easy to implement, the cost is low, and the prepared material can be applied to the fields of films and the like and reduces the use of plastic products to a certain extent.
Description
Technical Field
The invention belongs to the technical field of food packaging, and particularly relates to a eugenol-containing curdlan antioxidant film and a preparation method thereof.
Background
High-molecular packaging materials represented by plastics are widely applied to the food packaging industry due to the advantages of low cost, good mechanical properties and the like, and more than one third of the packaging materials are made of plastics. But because of the incomplete renewable utilization and the non-biodegradability of petroleum-based plastics, the petroleum-based plastics show great negative in sustainable development, and the development and use of biodegradable packaging materials are necessary requirements for ecologizing food packaging materials and better protecting ecological environment.
At present, the raw materials of the bio-based packaging film which is researched more mainly comprise starch, protein, gelatin-chitosan, polylactic acid, biological polyethylene and the like, and some materials can even be directly eaten. The polysaccharide is a water-insoluble extracellular polysaccharide produced by bacteria, has thermal gelation and non-toxic properties, and is widely used in the food industry and related fields. In addition, the excellent film-forming properties and biological properties of the available polysaccharides have attracted attention, and are now available as raw materials for bio-based films. In view of safety, additives in bio-based food packaging have tended in recent years to select natural compounds instead of synthetic agents. Eugenol (4-allyl-2-methoxyphenol) is a natural oily liquid, has been widely used in pharmaceuticals, cosmetics and foods due to its abundant resources and low price, and in addition, eugenol has a broad-spectrum antibacterial property and a high antioxidant activity, and incorporation of this natural compound into a bioactive film helps to protect against certain deterioration reactions, so it has attracted great interest in active packaging for food preservation.
Disclosure of Invention
The invention aims to provide a eugenol-containing available radical antioxidant film and a preparation method thereof, aiming at the defects of the prior art. The natural polysaccharide is selected as a membrane matrix, eugenol is added as a natural antioxidant, and the biological membrane which is safe and efficient and has an antioxidant function is prepared by mixing. In addition, the biofilm may reduce the use of potentially hazardous materials and to some extent, reduce the use of plastic products.
The purpose of the invention can be realized by the following technical scheme:
a eugenol-containing natural antioxidant film comprises the following raw material components: the polysaccharide of the native polysaccharide: 3-6% (w/v), tween 80: 0.5-1% (w/v), glycerin: 1-2% (w/v), eugenol: 0-0.24% (w/v), the balance being deionized water.
As a preferable aspect of the present invention, the raw material components of the oxidation resistant film include: 4 percent of native polysaccharide (w/v), 0.8 percent of tween (80) (w/v), 1.2 percent of glycerin (w/v), 0 to 0.24 percent of eugenol (w/v) and the balance of deionized water.
The preparation method of the eugenol-containing available radical antioxidant film comprises the following steps:
step 1, adding eugenol into deionized water, and magnetically stirring to dissolve the eugenol to form a eugenol solution;
step 2, adding the natural polysaccharide, the Tween 80 and the glycerol into the solution obtained in the step 1, and continuously stirring at normal temperature;
step 3, adding lactic acid into the solution obtained in the step 2 to adjust the pH value;
step 4, homogenizing the mixed solution obtained in the step 3 to form a uniformly distributed system;
step 5, pouring the membrane liquid obtained in the step 4 into a polytetrafluoroethylene plate, and placing the polytetrafluoroethylene plate aside for waiting for the liquid to be solidified into a membrane in a non-flowing state;
and 6, placing the film obtained in the step 5 in a constant temperature and humidity box, and demolding to obtain the film.
Further, the stirring time at normal temperature in the step 1 is 30-60 minutes.
Further, the stirring time in step 2 was 30 minutes.
Further, the pH is adjusted to 4 in step 3.
Further, the homogenization conditions in step 4 were 7000r/min, 2 minutes.
Further, the temperature of the polytetrafluoroethylene plate in step 5 was 100 ℃.
Further, in the step 6, the conditions of the constant temperature and humidity chamber are 25 ℃, 50% RH, and the standing time is 24 h.
Has the advantages that:
the invention selects the available polysaccharide as the substrate of the membrane material, which is a water-insoluble extracellular polysaccharide generated by bacteria, has the characteristics of thermal gelation, degradability and no toxicity, and is widely used in the food industry and related fields due to excellent film-forming property and biological property.
According to the invention, the plant essential oil component eugenol is used in the natural biomembrane, so that the green packaging film with oxidation resistance is obtained, which is beneficial to protecting and preventing certain deterioration reactions, and meanwhile, the application field of eugenol is widened.
Drawings
FIG. 1: technical route map
Table 1: performance of soluble film with different addition of eugenol concentration
Detailed Description
Example 1:
a eugenol-containing natural antioxidant film comprises the following raw material components: the polysaccharide is 4% (w/v), Tween (80) 0.8% (w/v), glycerol 1.2% (w/v), eugenol 0% (w/v), and the balance of deionized water.
Example 2:
a eugenol-containing natural antioxidant film comprises the following raw material components: the natural polysaccharide comprises 4% (w/v) of native polysaccharide, 0.8% (w/v) of tween (80), 1.2% (w/v) of glycerol, 0.02% (w/v) of eugenol and the balance of deionized water.
Example 3:
a eugenol-containing natural antioxidant film comprises the following raw material components: 4 percent of native polysaccharide (w/v), 0.8 percent of tween (80) (w/v), 1.2 percent of glycerin (w/v), 0.04 percent of eugenol (w/v) and the balance of deionized water.
Example 4:
a eugenol-containing natural antioxidant film comprises the following raw material components: the natural polysaccharide comprises 4% (w/v) of native polysaccharide, 0.8% (w/v) of tween (80), 1.2% (w/v) of glycerol, 0.08% (w/v) of eugenol and the balance of deionized water.
Example 5:
a eugenol-containing natural antioxidant film comprises the following raw material components: 4 percent of native polysaccharide (w/v), 0.8 percent of tween (80) (w/v), 1.2 percent of glycerin (w/v), 0.12 percent of eugenol (w/v) and the balance of deionized water.
Example 6:
a eugenol-containing natural antioxidant film comprises the following raw material components: 4 percent of native polysaccharide (w/v), 0.8 percent of tween (80) (w/v), 1.2 percent of glycerin (w/v), 0.16 percent of eugenol (w/v) and the balance of deionized water.
Example 7:
a eugenol-containing natural antioxidant film comprises the following raw material components: 4 percent of native polysaccharide (w/v), 0.8 percent of tween (80) (w/v), 1.2 percent of glycerin (w/v), 0.20 percent of eugenol (w/v) and the balance of deionized water.
Example 8:
a eugenol-containing natural antioxidant film comprises the following raw material components: 4 percent of native polysaccharide (w/v), 0.8 percent of tween (80) (w/v), 1.2 percent of glycerin (w/v), 0.24 percent of eugenol (w/v) and the balance of deionized water.
The preparation method comprises the following steps: firstly weighing eugenol, adding the eugenol into deionized water, then adding the curdlan, tween 80 and glycerol, magnetically stirring the membrane liquid at room temperature, then adjusting the pH with lactic acid, homogenizing, pouring into a polytetrafluoroethylene plate, placing in a constant temperature and humidity cabinet (25 ℃, 50% RH) for about 24h, and demolding to obtain the eugenol.
The performance of the obtained natural biofilm in examples 1 to 8 was tested, and the specific test method was as follows:
1. method for measuring thickness of film: a flat, dry, homogeneous film was selected, 6 points were randomly selected thereon, measured with a digital vernier caliper with a precision of 0.01mm, and then averaged, the units being expressed in mm.
2. The method for measuring the tensile strength and the elongation at break comprises the following steps: tensile strength (MPa) and ductility (%) of the film were measured using a physical property tester, with reference to the methods of GB/T1040.3-2006 "determination of tensile Properties of plastics" and Park, and modifications. The film samples were cut into rectangles (60 mm. times.20 mm), equilibrated at 50% humidity and 20 ℃ for at least 12h before testing, and tested immediately after sample removal. The film sample was fixed to a tensile jig (A/TG) of a physical property tester at a speed of 0.8mm/s with the jig set to 40 mm. 6 replicates were made for each sample. The formulas for tensile strength and elongation are shown below:
in the formula: TS is the tensile strength (MPa), F is the maximum tension (N) to which the film is subjected at break; t is the thickness (mm) of the film; w is the width (mm) of the film; EB is elongation at break (%), LmaxMaximum length (mm) reached at film break; l is0Is the initial length (mm) of the film.
3. The method for measuring the antioxidant activity comprises the following steps: 0.1g of biofilm was prepared and the sample was then added to 10mL of DPPH solution (0.1 mmoleL)-1DPPH in methanol). The solution was left at room temperature in the dark for 30 minutes. The absorbance (Abs) was then measured at 510nm using a spectrophotometer. DPPH free radical scavenging activity was calculated as percent inhibition according to the following formula:
DPPH inhibition ratio (%) ═ Abscontrol-Abssample)/Abscontrol))·100
Wherein AbscontrolIs the initial absorbance of DPPH solution (no membrane sample), whereas AbssampleIs the absorbance of the biofilm sample exposed to DPPH solution.
The performance of the obtained natural biofilm of examples 1 to 8 was tested according to the above test method, and the test results were as follows:
TABLE 1 Properties of natural films with different concentrations of eugenol addition
Experimental results show that the invention adopts the natural base as the substrate of the membrane material, and the eugenol essential oil is used for the membrane, so that the green packaging membrane with oxidation resistance and better mechanical property is obtained. The film helps to protect against certain deterioration reactions and reduces the use of plastic articles to some extent.
Claims (8)
1. The eugenol-containing available radical antioxidant film is characterized in that the raw material components of the antioxidant film comprise: the polysaccharide of the native polysaccharide: 4%, tween 80: 0.8%, glycerin: 1.2%, eugenol: 0.02%, the balance being deionized water, wherein, "%" is measured as w/v.
2. The method for preparing the eugenol-containing kuran-based antioxidant film as claimed in claim 1, wherein the step of: the method comprises the following steps:
step 1, adding eugenol into deionized water, and magnetically stirring to dissolve the eugenol to form a eugenol solution;
step 2, adding the natural polysaccharide, the Tween 80 and the glycerol into the solution obtained in the step 1, and continuously stirring at normal temperature;
step 3, adding lactic acid into the solution obtained in the step 2 to adjust the pH value;
step 4, homogenizing the mixed solution obtained in the step 3 to form a uniformly distributed system;
step 5, pouring the membrane liquid obtained in the step 4 into a polytetrafluoroethylene plate, and placing the polytetrafluoroethylene plate aside for waiting for the liquid to be solidified into a membrane in a non-flowing state;
and 6, placing the film obtained in the step 5 in a constant temperature and humidity box, and demolding to obtain the film.
3. The method of claim 2, wherein: the magnetic stirring time in step 1 is 30-60 minutes.
4. The method of claim 2, wherein: the stirring time in step 2 was 30 minutes.
5. The method of claim 2, wherein: in step 3 the pH is adjusted to 4.
6. The method of claim 2, wherein: the homogenization conditions in step 4 were 7000r/min, 2 minutes.
7. The method of claim 2, wherein: the temperature of the teflon plate in step 5 was 100 ℃.
8. The method of claim 2, wherein: in the step 6, the conditions of the constant temperature and humidity chamber are 25 ℃, 50% RH and the standing time is 24 h.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105111511A (en) * | 2015-07-31 | 2015-12-02 | 河南科技大学 | Antibacterial nano-composite edible film and preparation method therefor |
| CN108623852A (en) * | 2018-04-24 | 2018-10-09 | 黄正农 | A kind of edible package of instant food and preparation method thereof |
| CN109762208A (en) * | 2019-01-17 | 2019-05-17 | 大连大学 | The antibacterial food package film of edibility |
| WO2019147643A1 (en) * | 2018-01-25 | 2019-08-01 | Robertet, Inc. | Water dispersible fragranced films and use thereof |
| CN110845832A (en) * | 2019-10-21 | 2020-02-28 | 刘辉 | Biodegradable film for inner package of sanitary product |
-
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- 2020-08-07 CN CN202010787870.3A patent/CN111875855B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105111511A (en) * | 2015-07-31 | 2015-12-02 | 河南科技大学 | Antibacterial nano-composite edible film and preparation method therefor |
| WO2019147643A1 (en) * | 2018-01-25 | 2019-08-01 | Robertet, Inc. | Water dispersible fragranced films and use thereof |
| CN108623852A (en) * | 2018-04-24 | 2018-10-09 | 黄正农 | A kind of edible package of instant food and preparation method thereof |
| CN109762208A (en) * | 2019-01-17 | 2019-05-17 | 大连大学 | The antibacterial food package film of edibility |
| CN110845832A (en) * | 2019-10-21 | 2020-02-28 | 刘辉 | Biodegradable film for inner package of sanitary product |
Non-Patent Citations (4)
| Title |
|---|
| "Rapid Non-Destructive Quantification of Eugenol in Curdlan Biofilms by Electronic Nose Combined with Gas Chromatography-Mass Spectrometry";Lu Han et al.;《Sensors》;20200809(第20期);第1-14页 * |
| "可食性膜的研究进展";李彪等;《中国野生植物资源》;20170415;第36卷(第2期);第52-56页 * |
| 丁香酚/大豆蛋白膜对圣女果保鲜效果的研究;刘永等;《中国食品添加剂》;20170502(第04期);第83-87页 * |
| 不同精油单体的大豆分离蛋白可食膜对冷鲜猪肉的保鲜效果研究;张彬等;《中国食品学报》;20121231(第12期);第72-77页 * |
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