CN109265706A - A kind of modified corn alcohol soluble protein and preparation method thereof and antibacterial film preparation - Google Patents
A kind of modified corn alcohol soluble protein and preparation method thereof and antibacterial film preparation Download PDFInfo
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- CN109265706A CN109265706A CN201811097818.4A CN201811097818A CN109265706A CN 109265706 A CN109265706 A CN 109265706A CN 201811097818 A CN201811097818 A CN 201811097818A CN 109265706 A CN109265706 A CN 109265706A
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- soluble protein
- alcohol soluble
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- fish
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 48
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 45
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 45
- 240000008042 Zea mays Species 0.000 title claims abstract description 30
- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 30
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 title claims abstract description 29
- 235000005822 corn Nutrition 0.000 title claims abstract description 29
- 238000002360 preparation method Methods 0.000 title claims description 19
- 230000000844 anti-bacterial effect Effects 0.000 title abstract description 8
- 238000001814 protein method Methods 0.000 title description 3
- 229920002494 Zein Polymers 0.000 claims abstract description 25
- 241000251468 Actinopterygii Species 0.000 claims abstract description 24
- 239000005019 zein Substances 0.000 claims abstract description 19
- 229940093612 zein Drugs 0.000 claims abstract description 19
- 239000000843 powder Substances 0.000 claims abstract description 16
- UGTZMIPZNRIWHX-UHFFFAOYSA-K sodium trimetaphosphate Chemical compound [Na+].[Na+].[Na+].[O-]P1(=O)OP([O-])(=O)OP([O-])(=O)O1 UGTZMIPZNRIWHX-UHFFFAOYSA-K 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000003963 antioxidant agent Substances 0.000 claims abstract description 8
- 230000003078 antioxidant effect Effects 0.000 claims abstract description 8
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 claims abstract description 7
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 claims abstract description 7
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000005642 Oleic acid Substances 0.000 claims abstract description 7
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000012752 auxiliary agent Substances 0.000 claims abstract description 7
- 239000003899 bactericide agent Substances 0.000 claims abstract description 7
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 claims abstract description 7
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 claims abstract description 7
- 229960000935 dehydrated alcohol Drugs 0.000 claims abstract description 3
- 108010055615 Zein Proteins 0.000 claims description 23
- 238000004806 packaging method and process Methods 0.000 claims description 11
- 238000000576 coating method Methods 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 10
- 239000011248 coating agent Substances 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 8
- 229960004756 ethanol Drugs 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 4
- MDKCFLQDBWCQCV-UHFFFAOYSA-N benzyl isothiocyanate Chemical compound S=C=NCC1=CC=CC=C1 MDKCFLQDBWCQCV-UHFFFAOYSA-N 0.000 claims description 3
- 238000000502 dialysis Methods 0.000 claims description 3
- 231100000518 lethal Toxicity 0.000 claims description 3
- 230000001665 lethal effect Effects 0.000 claims description 3
- 239000008188 pellet Substances 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 210000001835 viscera Anatomy 0.000 claims description 3
- 150000002148 esters Chemical class 0.000 claims description 2
- 239000008367 deionised water Substances 0.000 claims 1
- 229910021641 deionized water Inorganic materials 0.000 claims 1
- 238000004821 distillation Methods 0.000 claims 1
- NWJGFNHFTLAZMR-XXAVUKJNSA-M sodium (Z)-octadec-9-enoate hydrate Chemical compound [OH-].[Na+].CCCCCCCC\C=C/CCCCCCCC(O)=O NWJGFNHFTLAZMR-XXAVUKJNSA-M 0.000 claims 1
- 235000013305 food Nutrition 0.000 abstract description 14
- 238000005259 measurement Methods 0.000 abstract description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract description 9
- 240000006394 Sorghum bicolor Species 0.000 abstract description 9
- 235000011684 Sorghum saccharatum Nutrition 0.000 abstract description 9
- 150000002978 peroxides Chemical class 0.000 abstract description 5
- 241000894006 Bacteria Species 0.000 abstract description 4
- 238000005033 Fourier transform infrared spectroscopy Methods 0.000 abstract description 3
- 230000003064 anti-oxidating effect Effects 0.000 abstract description 3
- 238000001142 circular dichroism spectrum Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 238000004132 cross linking Methods 0.000 abstract description 2
- 239000003431 cross linking reagent Substances 0.000 abstract description 2
- 238000003618 dip coating Methods 0.000 abstract description 2
- 230000035515 penetration Effects 0.000 abstract description 2
- 235000019441 ethanol Nutrition 0.000 description 25
- 239000000243 solution Substances 0.000 description 19
- IOLCXVTUBQKXJR-UHFFFAOYSA-M potassium bromide Chemical compound [K+].[Br-] IOLCXVTUBQKXJR-UHFFFAOYSA-M 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 239000012528 membrane Substances 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 241000252230 Ctenopharyngodon idella Species 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 235000013372 meat Nutrition 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000012460 protein solution Substances 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 3
- 235000019345 sodium thiosulphate Nutrition 0.000 description 3
- 239000012086 standard solution Substances 0.000 description 3
- 230000004888 barrier function Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000002983 circular dichroism Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 235000018927 edible plant Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 239000005022 packaging material Substances 0.000 description 2
- XNGIFLGASWRNHJ-UHFFFAOYSA-N phthalic acid Chemical compound OC(=O)C1=CC=CC=C1C(O)=O XNGIFLGASWRNHJ-UHFFFAOYSA-N 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 101000628535 Homo sapiens Metalloreductase STEAP2 Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 102100026711 Metalloreductase STEAP2 Human genes 0.000 description 1
- UEZVMMHDMIWARA-UHFFFAOYSA-N Metaphosphoric acid Chemical compound OP(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-N 0.000 description 1
- QAADZYUXQLUXFX-UHFFFAOYSA-N N-phenylmethylthioformamide Natural products S=CNCC1=CC=CC=C1 QAADZYUXQLUXFX-UHFFFAOYSA-N 0.000 description 1
- 206010028916 Neologism Diseases 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000004320 controlled atmosphere Methods 0.000 description 1
- XLJMAIOERFSOGZ-UHFFFAOYSA-M cyanate Chemical compound [O-]C#N XLJMAIOERFSOGZ-UHFFFAOYSA-M 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 239000012785 packaging film Substances 0.000 description 1
- 229920006280 packaging film Polymers 0.000 description 1
- 239000010816 packaging waste Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 239000000088 plastic resin Substances 0.000 description 1
- 239000004014 plasticizer Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 108060006613 prolamin Proteins 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000000518 rheometry Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
- C08H1/00—Macromolecular products derived from proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/14—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
- A23B4/18—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
- A23B4/20—Organic compounds; Microorganisms; Enzymes
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
- C08J5/18—Manufacture of films or sheets
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Manufacturing & Machinery (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
Abstract
A kind of modified corn alcohol soluble protein, including following components: zein powder, the sodium trimetaphosphate of purity 95%, dehydrated alcohol, sodium hydroxide, oleic acid, natural bactericidal agent, antioxidant and auxiliary agent.Corn (sorghum) alcohol soluble protein powder is modified by using crosslinking agent sodium trimetaphosphate (STMP), so as to improve the brittleness and other mechanical performances of film, optimizes best crosslinking condition.Using circular dichroism spectra, Fourier transform infrared spectroscopy characterizes the structure of protein films, and has detected its transparency, water solubility, extensibility, tensile strength, water penetration etc.；And show the zein solution dip-coating flesh of fish to form film, it cultivates at different temperature, the antibacterial and anti-oxidation characteristics of protein film is examined by measurement total plate count and peroxide value, food borne bacteria activity is inhibited to improve 50%~200%.
Description
Technical field
The invention belongs to bioprotein technical field of modification, in particular to arrive a kind of modified corn alcohol soluble protein and
Preparation method and antibacterial membrane preparation method.
Background technique
Plastics are applied very wide in food packaging, it has light-weight, convenient for molding and packaging operation, has good
Barrier, can also meet fresh food controlled atmosphere fresh-keeping packing certain air permeability require the advantages that.But the easy band of plastic products
Electrostatic and cause material surface pollute and heat-sealing etc. operating difficulties；The monomer molecule of certain plastic resins has certain toxicity,
When remaining monomer molecule in plastic resin, there is safe and healthy problem for food packaging.Phthalic acid in recent years
The discovery of the plasticisers such as ester is even more to cause people to the worry of its foodsafety.In addition, Plastics Packaging Wastes are difficult to degrade,
It is easy to cause problem of environmental pollution.
Protein material is from a wealth of sources, and because of its natural reproducible, biodegradable, the film raw material based on protein is close
It is gradually taken seriously over year.Amino acid profile and the space structure variation of protein very greatly, also result in corresponding biomembrane and add
The difference of the physics and chemical property of work method and formation film.And degradable films can as the barrier of solute and gas,
It keeps food quality and extends Food Shelf-life, so degradable package material causes the very big concern of food-processing industry.
Zein is a kind of natural edible plants macromolecular, has degradability, non-toxic, biocompatibility,
Hydrophobicity.Zein matrix membrane can have anti-oxidation function in the case where not adding antioxidant.In addition to packaging material
Outside, zein film biocompatibility with higher and adaptability, can be used for biological support, such as be implanted into the adsorbing base of cell
And as the physical support and molding guiding for forming neologism.Kafirin is one of sorghum prolamin,
Content in sorghum is 7-8%, is a kind of edible cheap protein resource of rich content, is a kind of hydrophobic proteins, is not easy
It is hydrolyzed by protease and carbohydrate-splitting enzyme, is suitable for the packaging of food.
But since corn (sorghum) alcohol soluble protein film brittleness is larger, and stickiness, extensibility and tensile strength are poor,
Mechanical performance is not met by needs of production.The research of currently available technology is mainly the operation of simple laboratory scale
With the application in food, such as film or the manual film that immerses form liquid by hand, it is difficult to large-scale production.Therefore egg is advanced optimized
White matter membrane preparation technology, it is necessary for improving the physico-chemical property of film.
Summary of the invention
In order to overcome the shortcomings in the background art, the purpose of the present invention is to provide a kind of modified corn alcohol soluble protein and its
Preparation method and antibacterial membrane preparation method.Using corn (sorghum) alcohol soluble protein as research object, by addition sodium trimetaphosphate and
Oleic acid is modified corn (sorghum) alcohol soluble protein, so as to improve the brittleness and other mechanical performances of protein films.Exist simultaneously
It wherein adds natural bactericidal agent, antioxidant and other auxiliary agents and produces food anti-bacterial packaging film, obtain its performance and fresh-keeping effect
To improving, to improve the shelf stability and safety of food product, there is preferable economic benefit and social value.
For achieving the above object, the present invention adopts the following technical scheme:
A kind of modified corn alcohol soluble protein, including following components:
Zein powder
The sodium trimetaphosphate of purity 95%
Dehydrated alcohol
Sodium hydroxide
Oleic acid
Natural bactericidal agent
Antioxidant
Auxiliary agent.
Zein powder, is dissolved in 80% ethanol solution, matched by a kind of preparation method of modified corn alcohol soluble protein
The zein powder solution of 2% (m/V) is made；0.1mol/LNaOH solution adjusting corn alcohol soluble protein powder pH value of solution is used again
The sodium trimetaphosphate of purity 95% is added in value, and oleic acid, natural bactericidal agent, antioxidant and other auxiliary agents after reaction, then are spent
Ionized water dialysis 12h, obtains modified corn alcohol soluble protein (Z-SP) product after pellet frozen is dry.
In order to be further improved technical solution, 0.1mol/LNaOH solution of the present invention is substituted with 0.1mol/LHCL.
A kind of application of modified corn alcohol soluble protein in fresh-keeping, applying step are as follows:
S1: flesh of fish pretreatment: after fresh fish is lethal, decaptitating truncates, removes fish scale, removes internal organ, with after scalding, uses
Distilled water rinsing, sloughs moisture；
S2: the preparation of coating liquid: the optimum proportioning of modified corn alcohol soluble protein is obtained by Responds Surface Methodology, by step
It is put into 10s in coating liquid by the pretreated flesh of fish in S1, taking-up is put into the pallet through ultraviolet-sterilization, to be filmed after dry；
S3: treated in the step S2 flesh of fish packaging: is added into the different sulphur of benzyl of 90 μ L/g by zeins amount
Cyanate is put into packaging bag.
Due to the adoption of the technical scheme as described above, the invention has the following beneficial effects: the present invention with corn (sorghum)
Alcohol soluble protein is research object, is modified by addition sodium trimetaphosphate and oleic acid to corn (sorghum) alcohol soluble protein, thus
Improve the brittleness and other mechanical performances of protein films.Protein antibacterial film is made of natural edible plants macromolecular, tool
There is degradability, non-toxic, preferable biocompatibility and hydrophobicity.This research is with zeins and kafirin
For research object, the membrance casting condition of zeins and kafirin has been inquired into.By using three metaphosphoric acid of crosslinking agent
Sodium (STMP) is modified corn (sorghum) alcohol soluble protein powder, and so as to improve the brittleness and other mechanical performances of film, optimization is most
Good crosslinking condition.Using circular dichroism spectra, Fourier transform infrared spectroscopy characterizes the structure of protein films, and it is transparent to have detected its
Degree, water solubility, extensibility, tensile strength, water penetration etc.；And show the zein solution dip-coating flesh of fish to form film,
It cultivates at different temperature, the antibacterial and anti-oxidation characteristics of protein film is examined by measurement total plate count and peroxide value, inhibit
Food borne bacteria activity improves 50%~200%.This has researched and developed novel packaging material for food, improves degradable compound
The properties of protein films are able to extend the shelf life of food.Research achievement is for promoting protein film to have in the food industry
Have wide practical use and use value is preserved the ecological environment more importantly reducing white pollution, there is important meaning
Justice.
Specific embodiment
By the following examples can the detailed explanation present invention, the open purpose of the present invention is intended to protect model of the present invention
Enclose all interior technological improvements.
Zein powder, is dissolved in 80% ethanol solution, matched by a kind of preparation method of modified corn alcohol soluble protein
The zein powder solution of 2% (m/V) is made；0.1mol/LNaOH solution adjusting corn alcohol soluble protein powder pH value of solution is used again
The sodium trimetaphosphate of purity 95% is added in value, and oleic acid, natural bactericidal agent, antioxidant and other auxiliary agents after reaction, then are spent
Ionized water dialysis 12h, obtains modified corn alcohol soluble protein (Z-SP) product after pellet frozen is dry.
It is as follows for the rheology measurement process of above embodiments:
Polymer (zein and Z-SP) solution to be measured that mass fraction is 2.0% is prepared by solvent of 80% ethyl alcohol, wherein
The pH value of solution is adjusted with 0.1mol/LHCl and NaOH solution.
Using the rheological curve, shear stress and viscoplasticity of rheometer measurement solution.In rheological curve measurement, taper plate
Using C60/1TIL, shear rate (D) range used is 0.1~1000s-1, testing time 1min.Viscoplasticity (but small oscillations
Shearing) measurement in, frequency (f) scanning range be 0.1~100Hz, shear stress (τ) be 0.1Pa (in linear viscoelastic region).Except examining
Examining temperature influences outside test, and test temperature is (25.0 ± 0.2) DEG C.
The response phase method of the preparation condition of Z-SP optimizes
According to the preparation method of protein solution, with the mass ratio (R of sodium trimetaphosphate and the molten egg of corn alcoholS/Z), reaction pH,
Reaction time is investigation condition, determines center combination test level according to single factor experiment result, using shear stress as response, is led to
It crosses data analysis and obtains optimum organization[13]。
Each factor of 1 center combination design of table and its level
Table 1 Each factor and its level of central composite design
Circular dichroism spectra measurement
Near ultraviolet sample liquid preparation process: claiming 0.2gZein and Z-SP to be dissolved in 80% ethanol solution respectively, and 2mg/mL egg is made
White solution；
Far ultraviolet sample liquid preparation process: 80% ethanol solution for taking two kinds of near ultraviolet protein solutions to be dissolved in respectively is made
0.2mg/mL protein solution.It is centrifuged after completely dissolution, the circular dichroism of the protein liquid is measured using circular dichroism spectrometer.
Measure wave-length coverage: 250~320nm of near ultraviolet, 190~260nm of far ultraviolet[14]。
Fourier transform infrared spectroscopy measurement
Zein the and Z-SP powder being freeze-dried in right amount is weighed, is allowed to the ratio of potassium bromide be 1:75, potassium bromide need to shift to an earlier date
Dry (105 DEG C, 4h or more) in an oven.The two is uniformly mixed in agate Yan Portland and is ground into fine powder, and suitable sample is taken to exist
It is measured after being pressed into transparent sheet in clean compression mold, and background single channel spectrum is surveyed with the tabletting of potassium bromide production, then
Sample channel spectrum, to obtain comparison spectrogram.
The above result of study shows: zeins is modified by phosphorylation, the enhancing of solution viscoplasticity, protein secondary
Apparent variation also has occurred in structure.Zein process is appropriate modified, can expand its application range in the market.
Modified corn alcohol soluble protein obtained in above-described embodiment is subjected to the fresh-keeping application of the flesh of fish, detailed process is as follows:
S1: fish pretreatment
After fresh and alive grass carp is lethal, decaptitating truncates, removes fish scale, removes internal organ, is split fish with by the tool of scalding
Meat is divided into consistent sample uniform in size (weight is depending on the fish index measured), is then rinsed, sloughed with distilled water
Moisture, grouping.(being divided into tri- groups of a, b, c, film, b, c as coating group, c do not add Benzy to a control group).
S2: coating method
Coating liquid is made in the optimal combination of the Zein film obtained by response surface[38], the flesh of fish cut is put into film
10s in liquid, taking-up are put into through drying in drying box, temperature 70 C or so in the ultraviolet pallet for killing bacterium.A few minutes stir one
Under, after being evaporated completely to solvent, sample surfaces will form the film of layer of transparent[39]。
S3: packaging and storage
Packaging bag and superclean bench are subjected to ultraviolet-sterilization 2h in advance, then packaging bag is wiped with alcohol, so
After packed.By the flesh of fish handled well (every piece weighs about 10g size), it is divided into tri- groups of a, b, c, a group not film as a control group,
For b group as coating group, c group is as coating group and adds benzyl isothiocyanate (additive amount is by the addition of zeins amount, 90 μ
L/g), three groups of samples respectively under the conditions of 4,15,25,37 DEG C storage 0,1,2,3,4,5,6, take out after 7d, measurement bacterium colony is total
Number.The flesh of fish handled well (every piece weighs about 300g) is divided into two groups of a, b, a group not film as a control group, b group as coating group,
Store 0 under the conditions of 4,15,25,37 DEG C to it, 1,3,5,7, take out after 9d, measure peroxide value, and under 4 DEG C of holding conditions
The flesh of fish quality carry out subjective appreciation[17,40]。
Maize alcohol-soluble protein film is fresh-keeping in the flesh of fish
(1) measurement of total plate count is the scissors after the fillet sample sterilizing after culture, fish in aseptic operating platform
Meat strand is diluted.It draws 10 μ L dilutions with liquid-transfering gun under each dilution to be added dropwise on agar plate, each processing parallel 3
Secondary test cultivates in 37 DEG C of constant incubators for 24 hours, carries out plate count.The foundation GB489.1- of total plate count measurement
2016[41]。
(2) measurement of peroxide value is operated according to GB5009.227-2016, calculation formula are as follows:
X1=(V-V0)×c×0.1269×100/m。
X1: peroxide value, unit are gram every hectogram (g/100g)；
V: the sodium thiosulfate standard solution volume of sample consumption, unit are milliliter (mL)；
V0: the sodium thiosulfate standard solution volume of blank test consumption, unit are milliliter (mL)；
C: the concentration of sodium thiosulfate standard solution, unit are mole every liter (mol/L)；
M: sample mass, unit are gram (g).
(3) subjective appreciation takes out grass carp of a, b, c group Jing Guo different disposal, carries out subjective appreciation from 4 DEG C of refrigerators.Its
Index is the color, smell, quality of the flesh of fish respectively.Score value is divided into 4 grades under each index, and specific standards are shown in Table 12.Scoring
Personnel be by professional training, the comprehensive scores of grass carp meat at 15~20 points be it is fresh, 10~15 points be that quality is good, 5-
10 points be quality it is general, 1~5 point be inferior quality.Each sample is in parallel three times.
12 subjective appreciation standard of table
Table 12 The sensory evaluation standards
Claims (4)
1. a kind of modified corn alcohol soluble protein, it is characterized in that: including following components:
Zein powder
The sodium trimetaphosphate of purity 95%
Dehydrated alcohol
Sodium hydroxide
Oleic acid
Natural bactericidal agent
Antioxidant
Auxiliary agent.
2. a kind of preparation method of modified corn alcohol soluble protein as described in claim 1, it is characterized in that: by zein powder
It is dissolved in 80% ethanol solution, is configured to the zein powder solution of 2% (m/V)；It is adjusted again with 0.1mol/LNaOH solution
Zein powder solution ph, be added purity 95% sodium trimetaphosphate, oleic acid, natural bactericidal agent, antioxidant and other
Auxiliary agent, after reaction, then with deionized water dialysis 12h, after pellet frozen is dry modified corn alcohol soluble protein (Z-SP) product.
3. the preparation method of modified corn alcohol soluble protein as claimed in claim 2, it is characterized in that: the 0.1mol/LNaOH
Solution is substituted with 0.1 mol/LHCL.
4. application of a kind of modified corn alcohol soluble protein as described in claim 1 in fresh-keeping, it is characterized in that: its applying step
Are as follows:
S1: flesh of fish pretreatment: after fresh fish is lethal, decaptitating truncates, removes fish scale, removes internal organ, with after scalding, with distillation
Water rinsing, sloughs moisture；
S2: the preparation of coating liquid: obtaining the optimum proportioning of modified corn alcohol soluble protein by Responds Surface Methodology, will be in step S1
It is put into 10s in coating liquid by the pretreated flesh of fish, taking-up is put into the pallet through ultraviolet-sterilization, to be filmed after dry；
S3: treated in the step S2 flesh of fish packaging: is added into the benzyl isothiocyanic acid of 90 μ L/g by zeins amount
Ester is put into packaging bag.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111286038A (en) * | 2020-04-09 | 2020-06-16 | 武汉轻工大学 | Preparation method and application of polyglycerol-modified zein |
| CN112773885A (en) * | 2021-03-19 | 2021-05-11 | 东北农业大学 | Zein disinfectant and preparation method thereof |
| CN113789100A (en) * | 2021-07-19 | 2021-12-14 | 河南省路嘉路桥股份有限公司 | Waterproof material for concrete matrix and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103937018A (en) * | 2014-04-30 | 2014-07-23 | 河南工业大学 | Preparation method of edible film with selective permeability |
| CN104262654A (en) * | 2014-09-22 | 2015-01-07 | 沈阳师范大学 | Preparation method of soybean protein isolate-modified zein composite film |
| CN106689354A (en) * | 2016-12-07 | 2017-05-24 | 浙江工业大学 | Phosphorylated soybean isolated protein composite coating agent as well as preparation method and application thereof |
-
2018
- 2018-09-20 CN CN201811097818.4A patent/CN109265706A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103937018A (en) * | 2014-04-30 | 2014-07-23 | 河南工业大学 | Preparation method of edible film with selective permeability |
| CN104262654A (en) * | 2014-09-22 | 2015-01-07 | 沈阳师范大学 | Preparation method of soybean protein isolate-modified zein composite film |
| CN106689354A (en) * | 2016-12-07 | 2017-05-24 | 浙江工业大学 | Phosphorylated soybean isolated protein composite coating agent as well as preparation method and application thereof |
Non-Patent Citations (9)
| Title |
|---|
| LEI-YAN WU等: "Wettability,surface microstructure and mechanical properties of films based on phosphorus oxychloride-treated zein", 《JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE》 * |
| 吴克刚等编著: "《食品微胶囊技术（第1版）》", 31 July 2006, 中国轻工业出版社 * |
| 吴磊燕: "玉米醇溶蛋白改性、界面特性及成膜性研究", 《中国博士学位论文全文数据库 工程科技Ⅰ辑》 * |
| 国家医药管理局中草药情报中心站编: "《植物药用有效成分手册（第1版）》", 31 March 1986, 人民卫生出版社 * |
| 张敏: "玉米醇溶蛋白的改性及成膜性质研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
| 李鸿梅等: "磷酸化修饰改善玉米醇溶蛋白溶解度", 《食品工业科技》 * |
| 毕崇浩: "《大豆功能性组分的分离和加工技术》", 31 July 2018, 天津科学技术出版社 * |
| 罗晶等: "玉米醇溶蛋白的磷酸化修饰及结构研究", 《现代食品科技》 * |
| 邵颖等主编: "《食品化学（第1版）》", 31 January 2018, 中国轻工业出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111286038A (en) * | 2020-04-09 | 2020-06-16 | 武汉轻工大学 | Preparation method and application of polyglycerol-modified zein |
| CN112773885A (en) * | 2021-03-19 | 2021-05-11 | 东北农业大学 | Zein disinfectant and preparation method thereof |
| CN113789100A (en) * | 2021-07-19 | 2021-12-14 | 河南省路嘉路桥股份有限公司 | Waterproof material for concrete matrix and preparation method thereof |
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