CN111875675B - Improved method for differentiation of epidermal stem cells into pancreatic cells - Google Patents
Improved method for differentiation of epidermal stem cells into pancreatic cells Download PDFInfo
- Publication number
- CN111875675B CN111875675B CN202010808793.5A CN202010808793A CN111875675B CN 111875675 B CN111875675 B CN 111875675B CN 202010808793 A CN202010808793 A CN 202010808793A CN 111875675 B CN111875675 B CN 111875675B
- Authority
- CN
- China
- Prior art keywords
- cells
- stem cells
- pancreatic
- epidermal stem
- differentiation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0676—Pancreatic cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/998—Proteins not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/09—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from epidermal cells, from skin cells, from oral mucosa cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- General Engineering & Computer Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010808793.5A CN111875675B (en) | 2018-09-03 | 2018-09-03 | Improved method for differentiation of epidermal stem cells into pancreatic cells |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811023578.3A CN109053866B (en) | 2018-09-03 | 2018-09-03 | Improved method for differentiation of epidermal stem cells into pancreatic cells |
CN202010808793.5A CN111875675B (en) | 2018-09-03 | 2018-09-03 | Improved method for differentiation of epidermal stem cells into pancreatic cells |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201811023578.3A Division CN109053866B (en) | 2018-09-03 | 2018-09-03 | Improved method for differentiation of epidermal stem cells into pancreatic cells |
Publications (2)
Publication Number | Publication Date |
---|---|
CN111875675A CN111875675A (en) | 2020-11-03 |
CN111875675B true CN111875675B (en) | 2021-10-01 |
Family
ID=64759286
Family Applications (6)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202010808793.5A Active CN111875675B (en) | 2018-09-03 | 2018-09-03 | Improved method for differentiation of epidermal stem cells into pancreatic cells |
CN202010792171.8A Active CN111848745B (en) | 2018-09-03 | 2018-09-03 | Improved method for differentiation of epidermal stem cells into pancreatic cells |
CN202010792164.8A Active CN111848743B (en) | 2018-09-03 | 2018-09-03 | Improved method for differentiation of epidermal stem cells into pancreatic cells |
CN202010808780.8A Active CN111875674B (en) | 2018-09-03 | 2018-09-03 | Improved method for differentiation of epidermal stem cells into pancreatic cells |
CN202010792168.6A Active CN111848744B (en) | 2018-09-03 | 2018-09-03 | Improved method for differentiation of epidermal stem cells into pancreatic cells |
CN201811023578.3A Active CN109053866B (en) | 2018-09-03 | 2018-09-03 | Improved method for differentiation of epidermal stem cells into pancreatic cells |
Family Applications After (5)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202010792171.8A Active CN111848745B (en) | 2018-09-03 | 2018-09-03 | Improved method for differentiation of epidermal stem cells into pancreatic cells |
CN202010792164.8A Active CN111848743B (en) | 2018-09-03 | 2018-09-03 | Improved method for differentiation of epidermal stem cells into pancreatic cells |
CN202010808780.8A Active CN111875674B (en) | 2018-09-03 | 2018-09-03 | Improved method for differentiation of epidermal stem cells into pancreatic cells |
CN202010792168.6A Active CN111848744B (en) | 2018-09-03 | 2018-09-03 | Improved method for differentiation of epidermal stem cells into pancreatic cells |
CN201811023578.3A Active CN109053866B (en) | 2018-09-03 | 2018-09-03 | Improved method for differentiation of epidermal stem cells into pancreatic cells |
Country Status (1)
Country | Link |
---|---|
CN (6) | CN111875675B (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113462636B (en) * | 2021-08-05 | 2023-11-14 | 合肥滴碧云生物科技有限公司 | Improved method for differentiating epidermal stem cells into liver cells |
Family Cites Families (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1253558C (en) * | 2003-09-02 | 2006-04-26 | 中国人民解放军第四军医大学口腔医学院 | Separation and culturing method of human epidermis stem cell |
CN1637137A (en) * | 2004-01-06 | 2005-07-13 | 中国人民解放军军事医学科学院野战输血研究所 | Method of inducing stem cell to differentiate to insulin-like cell and its application |
CN1772885B (en) * | 2004-11-11 | 2010-12-01 | 中国人民解放军军事医学科学院野战输血研究所 | Method of inducing stem cell to differentiate into pancreatic island-like cell and application of pancreatic island-like cell |
CN100425694C (en) * | 2005-04-15 | 2008-10-15 | 北京大学 | Method of inducing embryo stem cell to differentiate toward pancreatic cell |
CN101173249A (en) * | 2007-10-17 | 2008-05-07 | 暨南大学 | Method for inducing human fetal liver mesenchyma stem cell to pancreatic islet beta sample cell differentiation |
CN101603027A (en) * | 2008-06-12 | 2009-12-16 | 西北农林科技大学 | Method for inducing human bone marrow mesenchymal stem cells to differentiate into islet-like cells in vitro |
KR101025880B1 (en) * | 2008-10-23 | 2011-03-30 | 공희숙 | Method for the differentiation of human adult stem cells into insulin-secreting cells |
CN101580818A (en) * | 2009-03-09 | 2009-11-18 | 暨南大学 | Method for inducing the differentiation of mesenchymal stem cells of human embryo livers into islet beta-like cells and stably expressing insulin and special induced liquid thereof |
CN102311940A (en) * | 2010-07-09 | 2012-01-11 | 中国人民解放军军事医学科学院野战输血研究所 | Method for inducing differentiation of stem cells into islet-like cells |
CN102433300A (en) * | 2011-11-25 | 2012-05-02 | 厚朴生物科技(苏州)有限公司 | Method for constructing pancreatic stem cell line from human insulin and differentiating to insulin secretion cell |
CN102517248A (en) * | 2011-12-30 | 2012-06-27 | 中日友好医院 | In-vitro induced pancreas-islet-like structure forming method |
CN102618500A (en) * | 2012-03-21 | 2012-08-01 | 天津科技大学 | Method for inducing human mesenchymal stem cells to differentiate into insulin-secreting cells in vitro |
CN102618491B (en) * | 2012-03-21 | 2014-04-09 | 协和干细胞基因工程有限公司 | Culture solution for inducing mesenchymal stem cells to differentiate into islet-like cells, and inducing method and application of culture solution |
PL2844739T3 (en) * | 2012-04-30 | 2019-12-31 | University Health Network | METHODS AND COMPOSITIONS FOR GENERATING PANCREATIC PROGENITORS AND FUNCTIONAL BETA CELLS FROM hPSCs |
MX358590B (en) * | 2012-06-08 | 2018-08-24 | Janssen Biotech Inc | Differentiation of human embryonic stem cells into pancreatic endocrine cells. |
CN103146754A (en) * | 2013-02-04 | 2013-06-12 | 南通大学附属医院 | Method for differentiation of polygenic-modification induced pluripotent stem (iPS) cells into insulin secretion cells |
CN103194424A (en) * | 2013-03-28 | 2013-07-10 | 于涛 | Method for inducing embryonic stem cell into pancreatic tissue-like cells |
CN103740641A (en) * | 2013-12-26 | 2014-04-23 | 广州爱菲科生物科技有限公司 | Inducing culture medium and inducing method thereof for inducing human adipose-derived mesenchymal stem cell to be differentiated into islet-like cell |
CN103756954A (en) * | 2014-01-22 | 2014-04-30 | 青岛中天干细胞工程有限公司 | Inducer for inducing human adipose derived stromal cells into insulin-secretion cells, induction medium and method |
CN105462913A (en) * | 2014-09-05 | 2016-04-06 | 深圳市北科生物科技有限公司 | Method for inducing human umbilical cord mesenchymal stem cells to be differentiated into pancreatic beta cells |
CN104263697B (en) * | 2014-09-18 | 2017-08-04 | 胡振波 | A kind of method that inducing culture and induction human adipose mesenchymal stem cells generate insulin secretory cell |
CN104726395B (en) * | 2015-03-20 | 2018-03-23 | 深圳市人民医院 | It is a kind of to induce the method that people's induced multi-potent stem cell directed differentiation is pancreatic cell |
CN104862268A (en) * | 2015-05-27 | 2015-08-26 | 贵州北科泛特尔生物科技有限公司 | Culture medium for separating and inducing human adipose stem cells into pancreatic beta cells and use method of culture medium |
CN104988110A (en) * | 2015-07-08 | 2015-10-21 | 深圳爱生再生医学科技有限公司 | Method for transforming umbilical cord mesenchymal stem cells into islet cells |
CN105062952A (en) * | 2015-08-06 | 2015-11-18 | 深圳爱生再生医学科技有限公司 | Method for transformation of mesenchymal stem cells into islet cells |
CN105087468B (en) * | 2015-09-01 | 2018-12-07 | 东南大学 | A kind of method of efficient induction stem cell to pancreatic islet beta sample cell differentiation |
CN105670986A (en) * | 2015-11-23 | 2016-06-15 | 王意忠 | Culture medium for inducing human umbilical cord mesenchymal stem cells to differentiate into islet-like cells and induction method therefor |
CN106676056A (en) * | 2016-11-08 | 2017-05-17 | 里程 | Method for inducing differentiation from umbilical cord mesenchymal stem cells to insulin secretion cells |
CN106854638B (en) * | 2017-01-19 | 2019-12-27 | 天晴干细胞股份有限公司 | Method for inducing mesenchymal stem cells to differentiate into islet-like cells |
CN107267447A (en) * | 2017-06-18 | 2017-10-20 | 广东博溪生物科技有限公司 | A kind of enrichment method of epidermal stem cells |
CN107460158A (en) * | 2017-08-10 | 2017-12-12 | 河南省银丰生物工程技术有限公司 | A kind of inducing umbilical cord mesenchymal stem is divided into the method to form insulin |
-
2018
- 2018-09-03 CN CN202010808793.5A patent/CN111875675B/en active Active
- 2018-09-03 CN CN202010792171.8A patent/CN111848745B/en active Active
- 2018-09-03 CN CN202010792164.8A patent/CN111848743B/en active Active
- 2018-09-03 CN CN202010808780.8A patent/CN111875674B/en active Active
- 2018-09-03 CN CN202010792168.6A patent/CN111848744B/en active Active
- 2018-09-03 CN CN201811023578.3A patent/CN109053866B/en active Active
Also Published As
Publication number | Publication date |
---|---|
CN111848743A (en) | 2020-10-30 |
CN111848745A (en) | 2020-10-30 |
CN111848745B (en) | 2022-01-18 |
CN111848744B (en) | 2021-07-23 |
CN111848744A (en) | 2020-10-30 |
CN111848743B (en) | 2021-05-11 |
CN109053866B (en) | 2020-12-15 |
CN109053866A (en) | 2018-12-21 |
CN111875674B (en) | 2021-12-10 |
CN111875674A (en) | 2020-11-03 |
CN111875675A (en) | 2020-11-03 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101978044B (en) | Single pluripotent stem cell culture | |
CN101952415B (en) | The differentiation of human embryo stem cell | |
ES2665434T3 (en) | Differentiation of pluripotent stem cells using human feeder cells | |
IL181767A (en) | Scalable process for cultivating undifferentiated stem cells in suspension | |
KR101918817B1 (en) | Method for differentiation of hepatocyte derived from human stem cells and Hepatocyte | |
US20210054358A1 (en) | Method for making pluripotent stem cells | |
CN101724602B (en) | Method for the differentiation of human adult stem cells into insulin-secreting cells | |
Fang et al. | Differentiation of embryoid‐body cells derived from embryonic stem cells into hepatocytes in alginate microbeads in vitro 1 | |
US20130059378A1 (en) | Human epidermis-derived mesenchymal stem cell-like pluripotent cells and preparation method thereof | |
H Parsons et al. | Patents on technologies of human tissue and organ regeneration from pluripotent human embryonic stem cells | |
CN115975914A (en) | Method for inducing pluripotent stem cells by reprogramming of chemical small molecule drugs | |
Zhou et al. | Genetic modification of primate amniotic fluid-derived stem cells produces pancreatic progenitor cells in vitro | |
CN111875675B (en) | Improved method for differentiation of epidermal stem cells into pancreatic cells | |
CN113151147B (en) | Functional liver parenchymal cells and preparation method thereof | |
RU2430158C1 (en) | Method for differentiation of adult human stem cells in insulin-secreting cells | |
KR101766372B1 (en) | Method for Inducing Ectodermal Differentiation of Embryoid Bodies Derived from Human Pluripotent Stem Cells by CXCR2 Stimulation | |
Xiao et al. | Establishing a human pancreatic stem cell line and transplanting induced pancreatic islets to reverse experimental diabetes in rats | |
Lee et al. | Clump-passaging-based efficient 3D culture of human pluripotent stem cells under chemically defined conditions | |
KR20090050022A (en) | A method for differentiation of pluripotent stem cells to definitive endoderm cells | |
JP6956984B1 (en) | Blastocyst preparation method | |
Zhu et al. | Differentiation of Human umbilical cord-derived mesenchymal stem cells to melanocyte, Osteogenic and Neural cells in vitro | |
Aly et al. | Generation of Pancreatic β Cells from Peripheral Blood Mononucleocytes-Derived-Induced Pluripotent Stem Cells. | |
AU2010201431A1 (en) | Method for the differentiation of human adult stem cells into insulin-secreting cells | |
CA2701014A1 (en) | Method for the differentiation of human adult stem cells into insulin-secreting cells | |
CN116333971A (en) | Method for improving differentiation efficiency of human induced pluripotent stem cells to insulin-producing cells by co-culturing human mature islet cells and application |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information |
Inventor after: Xie Peizeng Inventor after: Yang Zhejun Inventor after: Deng Yin Inventor after: Yang Jun Inventor after: Zhang Yong Inventor before: Yang Jun Inventor before: Zhang Yong |
|
CB03 | Change of inventor or designer information | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20210914 Address after: 510700 Room 308, building 4, No. 2, scientific research road, Science City, Huangpu District, Guangzhou City, Guangdong Province Applicant after: Guangdong xinzhengcheng Life Science Co.,Ltd. Applicant after: Ningbo junshuo Technology Development Co.,Ltd. Address before: 471000 Luoyang economic and Technological Development Zone, Henan Province, south of Qianmen Street, south of Qianjiang street, 1 units 1-1404 of Yuejiang Road, 1 buildings. Applicant before: LUOYANG XUANZHI BIOLOGICAL TECHNOLOGY Co.,Ltd. |
|
TA01 | Transfer of patent application right | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20220120 Address after: 518000 room 2102, building 7, Qianhai excellence Financial Center (phase I), unit 2, guiwan area, Nanshan street, Qianhai Shenzhen Hong Kong cooperation zone, Shenzhen, Guangdong Patentee after: Shenzhen xinzhengcheng Life Science Research Institute Co.,Ltd. Address before: 510700 Room 308, building 4, No. 2, scientific research road, Science City, Huangpu District, Guangzhou City, Guangdong Province Patentee before: Guangdong xinzhengcheng Life Science Co.,Ltd. Patentee before: Ningbo junshuo Technology Development Co., Ltd |
|
TR01 | Transfer of patent right |