CN111870742A

CN111870742A - Preparation method of hydrophilic lubricating coating on surface of PVC (polyvinyl chloride) catheter

Info

Publication number: CN111870742A
Application number: CN202010590228.6A
Authority: CN
Inventors: 葛丽芹; 孙玲莉
Original assignee: Southeast University
Current assignee: Southeast University
Priority date: 2020-06-24
Filing date: 2020-06-24
Publication date: 2020-11-03

Abstract

The invention discloses a preparation method of a hydrophilic lubricating coating on the surface of a PVC catheter. Firstly, carrying out oxygen plasma treatment on the PVC catheter, then carrying out dopamine coating modification, and depositing a polydopamine layer on the surface layer of the PVC catheter. And finally, carrying out grafting modification by using PEG or PVA with amino of hydrophilic lubricating high molecular material with amino. The hydrophilic lubricating polymer with amino and the poly dopamine layer form a covalent bond by utilizing the Michael (Michael) addition or Schiff Base (Schiff's Base) principle so as to be firmly bonded on the surface of the PVC catheter. Because dopamine has high biocompatibility and the polydopamine layer and the PVC substrate can be firmly adhered together, the coating has good stability. PEG and PVA both belong to hydrophilic lubricating high molecular materials with good biocompatibility, so that the problems of hydrophobicity and large friction force on the surface of the PVC catheter can be solved. The method has the advantages of simple reaction process, mild conditions and easy operation.

Description

Preparation method of hydrophilic lubricating coating on surface of PVC (polyvinyl chloride) catheter

Technical Field

The invention relates to a preparation method of a hydrophilic lubricating coating on the surface of a PVC (polyvinyl chloride) catheter, belonging to the technical field of high polymer materials.

Background

The PVC catheter is called one of the popular types in clinical and household disposable catheters due to the advantages of excellent mechanical property, low cytotoxicity, better biocompatibility, low price and the like. But often makes the patient experience bad during use due to the hydrophobic and high friction of their surface, and also causes catheter-associated urinary tract infection (CAUTI). The existing improvement techniques are surface coating methods, such as applying a lubricant and coating methods, such as silver coating, antibacterial coating, hydrogel coating, etc. Compared with a surface coating method, the coating method has the advantages of convenience and simplicity in use, good effect and the like. However, the coating method is also limited by the aspects of coating adhesion, toxic and side effects or drug resistance of the coating, a manufacturing mode and the like.

Disclosure of Invention

The technical problem is as follows: the invention aims to provide a preparation method of a hydrophilic lubricating coating on the surface of a PVC catheter. Due to the strong adhesiveness of dopamine and the advantage of secondary reaction, the hydrophilic lubricating high polymer material with amino can be stably introduced to the surface of the PVC catheter in a chemical bond form. Because the selected dopamine and PEG or PVA are all substances with good biocompatibility, the coating has good biocompatibility. PEG or PVA has the characteristic of hydrophilic lubrication, so that the surface of the PVC catheter can also be endowed with the characteristic of hydrophilic lubrication.

The technical scheme is as follows: the invention provides a preparation method of a hydrophilic lubricating coating on the surface of a PVC catheter. The preparation method of the hydrophilic lubricating coating on the surface of the PVC catheter comprises the following steps: treating the PVC catheter by using oxygen plasma, coating and modifying the surface of the PVC catheter by using dopamine at room temperature, and finally performing modified grafting on the hydrophilic lubricating high polymer material with amino on the surface of the PVC catheter after the polydopamine deposition to obtain the hydrophilic lubricating coating.

Wherein the content of the first and second substances,

before grafting dopamine, the surface of the PVC catheter is firstly subjected to oxygen plasma treatment.

The plasma treatment time is 30-90 s.

The polydopamine is a medium conducting layer of a PVC catheter and a subsequent grafted high polymer material.

The concentration of the dopamine solution is 2 mg/ml.

The modification time of the dopamine is 6-28 h. For example, 6h, 12h, 18h, 28h, etc., but are not limited to the recited values, and other values not recited in the numerical range are also applicable.

The hydrophilic lubricating high-molecular material is PEG or PVA with amino, but is not limited to the two types, and can be other hydrophilic lubricating high-molecular materials with amino.

The grafting concentration of the macromolecular material such as PEG or PVA with amino is 1mg/ml-3 mg/ml. For example, 1mg/ml, 2mg/ml, 1.5mg/ml or 3mg/ml, etc., but the numerical values are not limited to those listed, and other numerical values not listed in the numerical range are also applicable.

The grafting temperature of the high molecular material such as PEG or PVA with amino is between 40 and 60 ℃. For example, 40 ℃, 45 ℃, 50 ℃ or 60 ℃, etc., but is not limited to the recited values, and other values not recited in the numerical range are also applicable.

The grafting time of the high polymer material such as PEG or PVA with amino is 6-28 h. For example, 6h, 12h, 18h, 28h, etc., but are not limited to the recited values, and other values not recited in the numerical range are also applicable.

The reaction of dopamine with PEG or PVA was performed in Tris-Hcl buffer at PH = 8-9.

The concentration of the Tris-HCl buffer solution is 0.01 mol/L.

Has the advantages that: the invention uses oxygen plasma to pretreat the surface of the PVC catheter, and then uses dopamine to coat and modify, so that the polydopamine layer is used as a mesoporous layer and is grafted with hydrophilic lubricating high molecular material PEG or PVA containing amino, and the prepared hydrophilic lubricating coating has the following advantages:

1. the method is simple and effective, the operation is simple and convenient, the reaction condition is mild, and the required equipment is simple.

2. The selected materials are all materials with good biocompatibility, and have small toxic and side effects on human bodies.

3. The prepared coating is hydrophilic and lubricating, has good stability, is convenient to use and can be used only by being wetted by deionized water.

The coating is surface-modified by dopamine and PEG or PVA high molecular materials with high biocompatibility, and the biocompatibility of the coating is relatively high. The dopamine has very strong adhesion performance, can firmly adhere to the surface of a PVC base material, and the PEG or PVA with amino is introduced into the surface of the PVC material modified by the polydopamine in a chemical bond form, so that the stability and the firmness of the surface of the coating are ensured. Meanwhile, the hydrophilic lubrication of the surface of the catheter can be improved by the hydrophilic lubrication of PEG or PVA, the comfort of a patient is improved, and the occurrence of CAUTI is reduced.

Drawings

FIG. 1 is a surface morphology diagram of a PVC catheter of the invention under a modified front and back electron microscope.

FIG. 2 is a block diagram of the graft-modified PVC catheter of the present invention (partial catheter).

Detailed Description

The invention is explained in more detail below with reference to exemplary embodiments and the accompanying drawings. The following examples are provided only for illustrating the present invention and are not intended to limit the scope of the present invention.

As shown in the figure, the PVC catheter is firstly subjected to oxygen plasma treatment, then is subjected to dopamine coating modification in a weak alkaline buffer solution at room temperature, and then is subjected to grafting modification with PEG or PVA with amino groups to form a hydrophilic lubricating coating. Wherein, fig. 1 is a surface morphology diagram of the PVC catheter under a modified front and back electron microscope, which is respectively as follows: VPVC is a bare tube; the OPVC/PDA is the surface modified by dopamine; OPVC/PDA/PEG is the surface of the material grafted with PEG with amino; the OPVC/PDA/PVA is the surface of the material after grafting the PVA with amino. FIG. 2 is a structural view of the PVC catheter of the present invention after graft modification (partial catheter), wherein a is a bare tube; b is a diagram after grafting of PEG with amino; c is a diagram after grafting of PVA with amino groups.

The preparation method specifically comprises the following steps:

the oxygen plasma is utilized to pretreat the PVC surface so as to introduce active groups, thereby facilitating subsequent grafting. By utilizing the super strong adhesion mechanism of dopamine, the polydopamine layer with the advantage of secondary reaction is firmly modified on the surface of the PVC catheter. And then, bonding a hydrophilic lubricating polymer material with amino groups, such as PEG or PVA, on the surface of the polydopamine coating of the PVC catheter in a covalent bond mode by utilizing a Schiff base principle and a Michael addition method to obtain the hydrophilic lubricating coating.

Specifically, in the first step, the PVC catheter is firstly subjected to oxygen plasma treatment for 50s, and is immersed in Tris-HCl buffer solution with the pH =8-9 and the dopamine concentration of 2mg/ml for modification for 6-28h at room temperature. Soaking the dopamine modified catheter in absolute ethyl alcohol for 30min, and washing the catheter with deionized water for 3 times to fully remove polydopamine and other impurities which are not firmly coated on the surface of the PVC catheter. Then modifying for 6-28h in the alkalescent Tris-HCl buffer solution with amino PEG or PVA concentration of 1-3mg/ml, wherein the modification process is carried out at the constant temperature of 40-60 ℃.

Example 1:

treating the PVC catheter by oxygen plasma for 50 s; immersing in Tris-HCl buffer (Tris concentration =0.01 mol/L) with pH =8-9 and dopamine concentration of 2mg/ml for modification at room temperature for 24 h; soaking the dopamine-modified catheter in absolute ethyl alcohol for 30min, and washing with deionized water for 3 times; and (3) putting the cleaned catheter into the Tris-HCl buffer solution with the amino PEG concentration of 2mg/ml, and modifying at the constant temperature of 50 ℃ for 24 hours.

Example 2:

treating the PVC catheter by oxygen plasma for 50 s; immersing in Tris-HCl buffer (Tris concentration =0.01 mol/L) with pH =8-9 and dopamine concentration of 2mg/ml for modification at room temperature for 28 h; soaking the dopamine-modified catheter in absolute ethyl alcohol for 30min, and washing with deionized water for 3 times; and (3) putting the cleaned catheter into the Tris-HCl buffer solution with the concentration of amino PVA being 1mg/ml, and modifying at the constant temperature of 50 ℃ for 12 hours.

Example 3:

treating the PVC catheter by oxygen plasma for 30 s; immersing in Tris-HCl buffer (Tris concentration =0.01 mol/L) with pH =8-9 and dopamine concentration of 2mg/ml for modification at room temperature for 6 h; soaking the dopamine-modified catheter in absolute ethyl alcohol for 30min, and washing with deionized water for 3 times; and (3) putting the cleaned catheter into the Tris-HCl buffer solution with the amino PEG concentration of 1.5mg/ml, and modifying at the constant temperature of 50 ℃ for 6 hours.

Example 4:

treating the PVC catheter by oxygen plasma for 90 s; immersing in Tris-HCl buffer (Tris concentration =0.01 mol/L) with pH =8-9 and dopamine concentration of 2mg/ml for modification at room temperature for 12 h; soaking the dopamine-modified catheter in absolute ethyl alcohol for 30min, and washing with deionized water for 3 times; and (3) putting the cleaned catheter into the Tris-HCl buffer solution with the amino PVA concentration of 3mg/ml, and modifying at the constant temperature of 50 ℃ for 28 h.

The technical means disclosed in the invention scheme are not limited to the technical means disclosed in the above embodiments, but also include the technical scheme formed by any combination of the above technical features. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and such improvements and modifications are also considered to be within the scope of the present invention.

Claims

1. A preparation method of a hydrophilic lubricating coating on the surface of a PVC catheter is characterized by comprising the following steps: firstly, carrying out oxygen plasma treatment on a PVC (polyvinyl chloride) catheter, then coating and modifying the surface of the PVC catheter by using dopamine at room temperature, and finally grafting a hydrophilic lubricating high polymer material with amino on the surface of the PVC catheter after polydopamine deposition to obtain the hydrophilic lubricating coating.

2. The method of claim 1, wherein the plasma treatment time is in the range of 30s to 90 s.

3. The method of claim 1, wherein the concentration of dopamine is 2 mg/ml.

4. The method of claim 1, wherein the coating modification time of dopamine is 6-28 h.

5. The method of claim 1, wherein the hydrophilic lubricating high molecular material is PEG or PVA with amino groups.

6. The method of claim 5, wherein the grafting concentration of the PEG or PVA polymer material with amino groups is 1mg/ml to 3 mg/ml.

7. The method of claim 5, wherein the grafting temperature of the amino group-containing PEG or PVA polymer material is between 40 and 60 ℃.

8. The method of claim 5, wherein the grafting time of the amino group-containing PEG or PVA polymer material is 6-28 h.

9. The method of claim 5, wherein the reaction of dopamine with PEG or PVA is performed in Tris-Hcl buffer at PH = 8-9.

10. The method of claim 9, wherein the concentration of Tris-Hcl buffer is 0.01 mol/L.