CN111869680B - Nano dry powder for disinfecting and self-cleaning floor of livestock and poultry house and application method thereof - Google Patents
Nano dry powder for disinfecting and self-cleaning floor of livestock and poultry house and application method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D5/00—Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced; Filling pastes
- C09D5/14—Paints containing biocides, e.g. fungicides, insecticides or pesticides
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D5/00—Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced; Filling pastes
- C09D5/16—Antifouling paints; Underwater paints
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D5/00—Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced; Filling pastes
- C09D5/16—Antifouling paints; Underwater paints
- C09D5/1687—Use of special additives
Abstract
The invention discloses a nano dry powder for disinfecting and self-cleaning the floor of a livestock and poultry house and an application method thereof, and the nano dry powder comprises the following components: 0.011-0.22 wt% of nano silver, 0.5-1.0 wt% of silver nitrate premix, 0.75-1.5 wt% of nano zinc oxide, 0.5-1.0 wt% of glucose, 80.0wt% of montmorillonite and 16.28-18.239 wt% of bran meal, wherein the silver nitrate premix is prepared by dissolving and diluting chemically pure silver nitrate, spraying, adsorbing and premixing with bran meal, and the silver content in the silver nitrate premix is 1.0 wt%. The dry sterilizing powder is applied to the ground of a farm, so that the ground has the functions of sterilization and self-cleaning, the ground has the effects of safety, slow release, lasting and broad-spectrum sterilization and virus killing, pathogenic microorganisms in the ground and the environment of a livestock and poultry house can be killed, the biosafety level is improved, and the occurrence of diseases is prevented and reduced.
Description
Technical Field
The invention belongs to the scientific field of animal health care, and relates to nano dry powder for enabling the ground of a livestock and poultry house to have disinfection and self-cleaning functions and an application method thereof, which are suitable for the ground environment of a livestock and poultry farm and are used for building the ground with the disinfection and self-cleaning functions.
Background
The biological safety of livestock and poultry is the key for ensuring the success of breeding, wherein the disinfection is the most important and most common measure for ensuring the biological safety. However, to date, despite the last hundred years of effort of global scientists, no commercial vaccine effective in controlling african swine fever has been developed all over the world, and biosafety is currently the only effective means.
At present, the most common means for improving the biological safety of colony houses in livestock and poultry breeding industry is to use a disinfectant to perform ground disinfection work, and no livestock and poultry ground and materials with disinfection and self-cleaning functions exist. With the scale and the higher intensification degree of the breeding industry, most of the breeding industry is continuous production, and the preventive disinfection with living bodies and without interrupting the production becomes a normal state. Most of the disinfectants (including phenols such as phenol, aldehyde disinfectants such as formaldehyde and polyformaldehyde, alkali disinfectants such as sodium hydroxide and calcium oxide, inorganic acid disinfectants such as sulfuric acid and hydrochloric acid, halogens such as chlorine-containing stone powder, chlorine dioxide and peroxides such as peracetic acid) used for the ground and the environment of livestock and poultry at present are not suitable for disinfecting the environment with living livestock and poultry; the disinfectant which is most commonly used for living bodies of livestock and poultry and living bodies at present is quaternary ammonium salt cationic surfactant (such as benzalkonium bromide, also known as benzalkonium bromide) which can be used for disinfecting pig bodies, has strong and quick bactericidal action, weak irritation and low toxicity, but has the defects of great influence by organic matters and poor disinfection effect on ground environments such as animal houses and the like.
In addition, the disinfectants widely used at present are diluted by water and sprayed for disinfection, so that air and the ground are wet, the growth of the pigs is not facilitated, most disinfectants cannot last for a long time (most disinfectants do not exceed 7 days) after disinfection, particularly, the disinfectants are disinfected once every 3 to 5 days at the epidemic disease circulation stage, and even every day, so that the workload is large, the cost is high, the stress of the pigs is large, and the growth and the health are not facilitated.
If the ground of the livestock and poultry house has the functions of disinfection and self-cleaning, the defects of the traditional disinfection can be avoided, the disinfection times are reduced, and the biological safety level is greatly improved.
Disclosure of Invention
In order to solve the technical problems of the existing disinfectant for livestock and poultry breeding places, the invention aims to provide the nano dry powder for enabling the ground of the livestock and poultry house to have the disinfection and self-cleaning functions, the nano dry powder is suitable for being used for building the ground with the disinfection and self-cleaning functions for the livestock and poultry breeding places, the disinfection and self-cleaning functions of the ground of the livestock and poultry house are achieved, the traditional ground environment disinfection is replaced, the nano dry powder is suitable for large-scale and intensive breeding industry, production does not need to be interrupted, the disinfected self-cleaning ground has safe, slow-release and lasting broad-spectrum disinfection and virus-killing effects, pathogenic microorganisms in the livestock and poultry ground and the environment can be killed, the biological safety level is improved, and the occurrence of diseases is prevented and reduced.
In order to achieve the technical purpose, the invention provides nano dry powder for providing disinfection and self-cleaning functions on the ground of a livestock and poultry house, which comprises the following components:
the silver nitrate premix is prepared by dissolving and diluting chemically pure silver nitrate, spraying, adsorbing with bran meal, and premixing uniformly, wherein the silver content in the silver nitrate premix is 1.0 wt%.
The nano silver is simple substance silver particles with the average particle size of 5-50nm, and the purity is not lower than 99 percent; the average grain diameter of the nano zinc oxide is 25 nm; the montmorillonite is used for nano-scale feed or medical use, and the content of the montmorillonite is not less than 90%; the content of the bran meal is 100%.
The raw materials are all common products which can be purchased conveniently in the market.
The invention also provides a method for spraying the nano dry powder for disinfecting and self-cleaning the floor of the livestock and poultry house into the floor or environment of the livestock and poultry house according to the amount of 1kg/50-100 square meters, the nano dry powder is repeatedly sprayed once in 3-5 days, and the nano dry powder is repeatedly sprayed once in 1kg/100 square meters every 20-30 days later.
It should be noted that if the ground of the livestock and poultry house is thoroughly washed with water, the water is sprayed once more according to the amount of 1kg/50-100 square meters, the spraying is repeated once after 5 days, and the spraying is repeated once again according to the amount of 1kg/100 square meters every 20-30 days.
The principle of the invention is as follows:
the invention aims to realize that the ground environment of the livestock and poultry house has the functions of disinfection and self-cleaning and improves the biological safety level.
The nano silver particles contained in the nano dry powder have the effects of no toxicity, harmlessness, slow release and lasting broad-spectrum sterilization and virus killing (particularly have a good effect of killing DNA large viruses with envelope such as herpes virus, influenza virus and the like), the nano dry powder is sprayed to ensure that the nano silver particles are uniformly attached to the ground of a livestock house, and the silver particles attached to the ground play the functions of slow release and lasting broad-spectrum sterilization and virus killing.
Silver ions and simple substance silver in the form of Eimeria (0.32nm) have quick, broad-spectrum and stronger bactericidal and virucidal functions. The nano dry powder is added with a small amount of silver nitrate and glucose, except for utilizing the functions of quick sterilization and virus killing of silver ions, when the nano dry powder is sprayed on the ground of a livestock and poultry house, a certain amount of ammonia gas can be generated due to the fact that a proper amount of excrement is arranged on the ground of the livestock and poultry house, silver ions and the ammonia gas react to form silver ammonia ions, and then the silver ammonia ions and the glucose in the product can react to separate out simple substance of the Hermitian silver.
The nano zinc oxide contained in the nano dry powder also has extremely strong antibacterial and bacteriostatic functions, and can perform oxidation reaction with various organic matters (including organic matters in bacteria) so as to kill the bacteria. Especially under the condition of illumination, the bacteriostatic and virucidal capabilities are stronger. The montmorillonite for feed or medicine can ensure the dispersion performance of the product, when the product is sprayed on the ground uniformly, the nano zinc oxide particles in the product are uniformly attached to the ground of a livestock house, and cooperate with nano silver, angstrom silver and silver ions to expand the antibacterial spectrum and reduce the usage amount of the nano silver.
The nano dry powder is uniformly sprayed on the ground of the livestock and poultry house, so that the livestock and poultry house has the functions of disinfection and self-cleaning; the disinfection self-cleaning function and efficacy of the product are based on African swine fever virus, and the nanometer silver saturation degree of attack against the African swine fever virus is not less than 1 time, and the silver ion or simple substance silver particle saturation degree of attack is 1.74 multiplied by 105To 3.47X 105And (4) doubling.
The invention has the beneficial effects that:
(1) by adopting the product and the method, the ground of the livestock and poultry house does not need to be specially modified, the large-scale and intensive breeding industry can quickly realize the disinfection and self-cleaning function of the ground environment of the livestock and poultry house, and the product and the method have low cost and safety.
(2) The invention provides a simple method for realizing the livestock and poultry house ground which is suitable for continuous production and has the function of disinfection and self-cleaning for large-scale and intensive breeding industry, and the nano silver, the nano zinc oxide, the silver nitrate and the angstrom silver generated by skillfully utilizing the ammonia gas on the livestock and poultry house ground synergistically exert the quick and efficient killing effect on pathogenic microorganisms in the environment, and meanwhile, the ground also has the slow-release, lasting and safe broad-spectrum sterilization and virus killing effects, and is low in cost and convenient to use.
(3) The security of this product is strong: firstly, the safety of the nano silver particles and the nano zinc oxide is recognized by the international medical field; secondly, the silver ion concentration of the silver nitrate in the product is 50-100mg/kg, the silver nitrate has certain toxicity (the mouse LD50 is 50mg/kg), and the environment can be polluted, but the maximum using amount of the product is 20 g/square meter, the maximum area of each pig in large-scale feeding is not more than 1.5 square meter taking fattening pigs (more than 15 kg) as an example, assuming that one pig completely eats all the ground nano dry powder at one time, the amount of the ingested ionic silver is 1/1000-1/500 of half lethal dose, and the maximum area is in an absolute safety range. In addition, because the ground of the livestock and poultry house is provided with a proper amount of excrement, certain ammonia gas can be generated, silver ions react with the ammonia gas to form silver ammonia ions, and the silver ammonia ions react with glucose in the product to precipitate simple substance silver for detoxification.
(4) The nano dry powder is uniformly sprayed on the ground of the livestock and poultry house, so that the livestock and poultry house has the functions of disinfection and self-cleaning; the disinfection self-cleaning function and efficacy of the product are based on the African swine fever virus, and the disinfection and self-cleaning functions are based on the African swine fever virus, so that the nano silver saturation degree of attack against the African swine fever virus is ensured to be more than 1-5 times, and the silver ion or simple substance silver particle saturation degree of attack is 1.74 multiplied by 105To 3.47X 105And (4) doubling.
Drawings
FIG. 1 is a graph showing the results of the Escherichia coli inhibition experiment in example 7, in which the negative control group was prepared without adding the nano sterile dry powder, and the experimental group was prepared with adding the nano sterile dry powder of example 4 of the present invention.
FIG. 2 is a graph showing the results of the Salmonella inhibition experiment in example 8, in which the negative control group was prepared without adding the nano sterilizing dry powder, and the experimental group was prepared with adding the nano sterilizing dry powder of example 4 of the present invention.
FIG. 3 is a graph showing the results of the streptococcal inhibition test in example 9, in which the nano disinfectant dry powder of example 4 of the present invention was added to the negative control group and the nano disinfectant dry powder of the present invention was added to the test group.
FIG. 4 is a graph showing the results of analysis of the composition of the genus of microorganisms in the dust of the hog house in example 10.
FIG. 5 is a graph showing the results of analysis of the composition of the genus level of microorganisms in feces of the piggery in example 11.
Detailed Description
The following examples and examples are intended to further illustrate the invention, but are not intended to limit the invention.
The pathogen local area defined by the invention is the average area of the viruses or bacteria projected to a plane, the average diameter of the African swine fever virus is 200nm, and the local area of the African swine fever virus is 31400nm2(ii) a The pathogenic local area unit defined by the invention refers to the number of pathogenic local area units per square meter, such as 3.2 multiplied by 10 of African swine fever virus local area units per square meter13(ii) a The saturation attack degree of the nano silver or the silver ions defined by the invention is the ratio of the number of the nano particles or the number of the silver ions to the pathogen local unit, and 1 is defined as 1 nano silver particle in 1 African swine fever virus local unit. For example, the particle size is about 6.8X 10 by weight of 5nm silver particles-16mg, 20nm silver particles weight about 4.396X 10-14mg, 50nm weight about 6.87X 10-13mg, assuming that the saturation attack degree is 1 time, 5nm silver 0.022mg, 20nm silver 1.4mg and 50nm silver 22mg are needed per square meter, and the addition amounts are 0.011%, 0.07% and 0.11% respectively according to the first use amount of the product of 20 g.
Example 1
A nanometer dry powder for disinfecting and self-cleaning the ground of livestock and poultry houses comprises the following components:
the nano silver is 5 nm;
the silver nitrate premix is prepared by dissolving and diluting chemically pure silver nitrate, spraying, adsorbing with bran meal, and premixing uniformly, wherein the silver content in the silver nitrate premix is 1 wt%.
The above nano dry powder is uniformly sprayed according to 20 g/square meter, so that the saturation attack degree of the nano silver particles on the ground of the livestock and poultry house for African swine fever viruses is 1 time, and the saturation attack degree of the silver ions is 3.47 multiplied by 105And (4) doubling.
Example 2
A nanometer dry powder for disinfecting and self-cleaning the ground of livestock and poultry houses comprises the following components:
the nano silver is 5 nm;
the silver nitrate premix is prepared by dissolving and diluting chemically pure silver nitrate, spraying, adsorbing with bran meal, and premixing uniformly, wherein the silver content in the silver nitrate premix is 1 wt%.
The above nano dry powder is uniformly sprayed according to 20 g/square meter, so that the saturation attack degree of the nano silver particles on the ground of the livestock and poultry house against African swine fever viruses is 5 times, and the saturation attack degree of the silver ions is 1.73 multiplied by 105And (4) doubling.
Example 3
A nanometer dry powder for disinfecting and self-cleaning the ground of livestock and poultry houses comprises the following components:
the nano silver is 20 nm;
the silver nitrate premix is prepared by dissolving and diluting chemically pure silver nitrate, spraying, adsorbing with bran meal, and premixing uniformly, wherein the silver content in the silver nitrate premix is 1 wt%.
The above nano dry powder is uniformly sprayed according to 20 g/square meter, so that the saturation attack degree of the nano silver particles on the ground of the livestock and poultry house for African swine fever viruses is 1 time, and the saturation attack degree of the silver ions is 3.47 multiplied by 105And (4) doubling.
Example 4
A nanometer dry powder for disinfecting and self-cleaning the ground of livestock and poultry houses comprises the following components:
the nano silver is 20 nm;
the silver nitrate premix is prepared by dissolving and diluting chemically pure silver nitrate, spraying, adsorbing with bran meal, and premixing uniformly, wherein the silver content in the silver nitrate premix is 1 wt%.
The above nano dry powder is uniformly sprayed according to 20 g/square meter, so that the saturation attack degree of the nano silver particles on the ground of the livestock and poultry house for African swine fever virus is 1.5 times, and the saturation attack degree of the silver ions is 3.47 multiplied by 105And (4) doubling.
Example 5
A nanometer dry powder for disinfecting and self-cleaning the ground of livestock and poultry houses comprises the following components:
the nano silver is 50 nm;
the silver nitrate premix is prepared by dissolving and diluting chemically pure silver nitrate, spraying, adsorbing with bran meal, and premixing uniformly, wherein the silver content in the silver nitrate premix is 1 wt%.
The above nano dry powder is uniformly sprayed according to 20 g/square meter, so that the saturation attack degree of the nano silver particles on the ground of the livestock and poultry house for African swine fever viruses is 1 time, and the saturation attack degree of the silver ions is 3.47 multiplied by 105And (4) doubling.
Example 6
A nanometer dry powder for disinfecting and self-cleaning the ground of livestock and poultry houses comprises the following components:
the nano silver is 50 nm;
the silver nitrate premix is prepared by dissolving and diluting chemically pure silver nitrate, spraying, adsorbing with bran meal, and premixing uniformly, wherein the silver content in the silver nitrate premix is 1 wt%.
The above nano dry powder is uniformly sprayed according to 20 g/square meter, so that the saturation attack degree of the nano silver particles on the ground of the livestock and poultry house for African swine fever viruses is 2 times, and the saturation attack degree of the silver ions is 3.47 multiplied by 105And (4) doubling.
Example 7
Mixing the above nanometer sterilizing dry powder at a ratio of 100m2The dosage of 1kg is uniformly sprayed on a bacteria culture dish (57 cm)2) Then inoculated at about 3X104Escherichia coli (G) cfu/ml-Bacteria) 100ul, placed in a bacteria incubator for 14 hours of inverted culture, as shown in fig. 1: the negative control group is not added with the nano disinfection dry powder, the colony number in the three culture dishes is more than 300, and the three culture dishes are completely grown in a sterile manner in the experimental group sprayed with the nano disinfection dry powder. The nano disinfection coating has obvious sterilization and bacteriostasis effects on escherichia coli.
Example 8
Mixing the above nanometer sterilizing dry powder at a ratio of 100m2The dosage of 1kg is uniformly sprayed on a bacteria culture dish (57 cm)2) Then inoculated at about 4X104100ul of cfu/ml Salmonella (G-bacterium) were placed in a bacterial incubator and cultured upside down for 12 hours as shown in FIG. 2: the negative control group is not added with the nano disinfection dry powder, the number of bacterial colonies in the two culture dishes is more than 300, and the experimental group sprayed with the nano disinfection dry powder has the advantage that the two culture dishes completely grow in a sterile manner. The nano disinfection coating has obvious sterilization and bacteriostasis effects on salmonella.
Example 9
Will be above mentionedSterilizing dry powder of rice 100m2The dosage of 1kg is uniformly sprayed on a bacteria culture dish (57 cm)2) Then inoculated at about 3X104cfu/ml Streptococcus (G)+Bacteria) 100ul, placed in a bacteria incubator for 12 hours of inverted culture, as shown in fig. 3: the negative control group is not added with the nano disinfection dry powder, the colony number in the three culture dishes is more than 300, and the three culture dishes are completely grown in a sterile manner in the experimental group sprayed with the nano disinfection dry powder. The nano disinfection coating has obvious effects of sterilization and bacteriostasis on streptococcus.
Example 10
Mixing the above nanometer sterilizing dry powder at a ratio of 100m2The dosage of 1kg is uniformly sprayed in a nursery pig house of a pig farm, meanwhile, a negative control group is set to carry out no treatment, 6 samples are randomly extracted from the ground dust of the piggery of an experimental group (PC.Dust) and a negative control group (NC.Dust) after 3 hours, and the diversity analysis is carried out on the ground microorganisms of the piggery by utilizing a high-throughput sequencing technology.
According to the statistical table (table 1) of Alpha diversity index values among the piggery dust groups, the coverage rate of 6 piggery dust sample libraries is over 99%, which shows that the sequencing result better reflects the real situation of the samples. The Ace, Chao 1, Simpson and shanoon indexes of the experimental group are smaller than those of the negative control group, which shows that the abundance and diversity of microorganism species in dust in a pigsty are obviously reduced by using the nano disinfection dry powder disclosed by the invention. After the microorganisms in the dust of two groups of piggeries are analyzed in a genus-level composition structure (figure 4), the dominant bacteria in the two groups are both lactobacillus, and the experimental groups increase the relative abundance of lactobacillus, psychrophilus, carnobacterium and aerococcus, which shows that the relative abundance of beneficial bacteria in the dust of the piggeries is improved by using the nano disinfection dry powder disclosed by the invention, and the microbial community distribution is more uniform.
TABLE 1 pig house dust 6 samples Alpha diversity index statistics (group as unit)
Example 11
Mixing the above nanometer sterilizing dry powder at a ratio of 100m2The using dosage of 1kg is uniformly sprayed in a nursery pig pigsty of a pig farm, meanwhile, a negative control group is set to carry out no treatment, 12 samples are randomly extracted from ground excrement in the pigsty of an experimental group (PC. Faeces) and a negative control group (NC. Faeces) after 3 hours, and the diversity analysis is carried out on the microorganisms on the opposite side of the pigsty by utilizing a high-throughput sequencing technology.
According to the Alpha diversity index value statistical table (table 2) among piggery manure groups, the coverage rate of 12 piggery manure sample libraries is over 99%, which shows that the sequencing result better reflects the real situation of the samples. The Ace, Chao 1, Simpson and shanoon indexes of the experimental group are lower than those of the negative control group, so that the abundance and diversity of microorganism species in the piggery manure are obviously reduced by using the nano disinfection dry powder disclosed by the invention. After microorganisms in the excrement of two groups of piggeries are analyzed according to a genus-level composition structure (figure 5), the dominant bacteria in the two groups are lactobacillus and clostridium, and the experimental group improves the relative abundance of beneficial bacteria such as lactobacillus and clostridium and reduces the abundance of harmful bacteria such as acinetobacter, oligobacter, streptococcus and methanobrevibacterium; the nano disinfection dry powder of the invention improves the relative abundance of some beneficial bacteria in the pigsty excrement and effectively reduces the abundance of harmful bacteria.
TABLE 2 pig house feces 12 samples Alpha diversity index statistics (group is unit)
Claims (2)
1. The nano dry powder for disinfecting and self-cleaning the floor of the livestock and poultry house is characterized by comprising the following components:
0.011 percent to 0.22 percent of nano silver;
0.5-1.0 wt% of silver nitrate premix;
0.75 to 1.5 weight percent of nano zinc oxide;
0.5 to 1.0 weight percent of glucose;
80.0wt% of montmorillonite;
the silver nitrate premix is prepared by dissolving and diluting chemically pure silver nitrate, spraying, adsorbing with bran meal, and premixing uniformly, wherein the silver content in the silver nitrate premix is 1 wt%;
and then adding 16.28-18.239 wt% of bran meal;
the nano silver is simple substance silver particles with the average particle size of 5-50nm, and the purity is not lower than 99 percent; the average grain diameter of the nano zinc oxide is 25 nm; the montmorillonite is used for nano-scale feed or medical use, and the content of the montmorillonite is not less than 90%; the content of the bran meal is 100%.
2. The nano dry powder for providing the livestock and poultry house floor with the functions of disinfection and self-cleaning as claimed in claim 1, is characterized in that: the nano dry powder is sprayed on the ground or the environment of the livestock and poultry according to the amount of 1kg/50-100 square meters, and is repeated once in 3-5 days, and is repeated once in every 20-30 days according to the amount of 1kg/100 square meters.
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| CN105217749A (en) * | 2015-07-03 | 2016-01-06 | 何素梅 | Absorption carrier of a kind of loading nano silvery and preparation method thereof |
| CN105494442A (en) * | 2015-12-29 | 2016-04-20 | 苏州市阳澄湖三家村蟹业有限公司 | Aquaculture bactericide |
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2020
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| CN101999409A (en) * | 2010-09-30 | 2011-04-06 | 王平康 | Nano silver-zinc complex antibacterial disinfectant and preparation method thereof |
| CN103416342A (en) * | 2012-05-16 | 2013-12-04 | 鹤山市雷迅新农机械科技有限公司 | Underwater oxygenation energy aeration pipe applied to aquaculture |
| CN104026150A (en) * | 2014-05-30 | 2014-09-10 | 湖南省轻工投资有限公司 | Composite liquid for preventing and killing avian influenza viruses and preparation method and application thereof |
| CN105217749A (en) * | 2015-07-03 | 2016-01-06 | 何素梅 | Absorption carrier of a kind of loading nano silvery and preparation method thereof |
| CN105494442A (en) * | 2015-12-29 | 2016-04-20 | 苏州市阳澄湖三家村蟹业有限公司 | Aquaculture bactericide |
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