CN111838179A - Turbulence-enhanced continuously-prepared broad-spectrum drug-resistant bacterium killing preparation as well as preparation method and application thereof - Google Patents
Turbulence-enhanced continuously-prepared broad-spectrum drug-resistant bacterium killing preparation as well as preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F1/00—Metallic powder; Treatment of metallic powder, e.g. to facilitate working or to improve properties
- B22F1/05—Metallic powder characterised by the size or surface area of the particles
- B22F1/054—Nanosized particles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F9/00—Making metallic powder or suspensions thereof
- B22F9/16—Making metallic powder or suspensions thereof using chemical processes
- B22F9/18—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds
- B22F9/24—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
Abstract
The invention discloses a method for continuously preparing broad-spectrum drug-resistant bacteria killing preparation by turbulence enhancement and application thereof. The method is simple and rapid to operate, green and environment-friendly, and easy for large-scale production, and the prepared metal antibacterial nanoparticles have the advantages of small particles, large specific surface area, no toxic and harmful residues and the like. In addition, the prepared sterilizing agent has the function of broad-spectrum killing of drug-resistant bacteria, and can be applied to various fields which are easily polluted by bacteria.
Description
Technical Field
The invention relates to a turbulence-enhanced continuously-prepared broad-spectrum drug-resistant bacterium killing preparation, a preparation method and application thereof, belonging to the advanced manufacturing field of sterilization preparations.
Background
Chinese antibiotics account for about half of the world, 48% of which are used for human and the rest for agriculture. In recent years, environmental pollution caused by abuse and discharge of antibiotics is promoting bacterial evolution and drug resistance, so that various drug-resistant bacteria (such as methicillin-resistant staphylococcus aureus (MRSA), vancomycin-resistant staphylococcus aureus (VRSA), vancomycin-resistant enterococcus (VRE), penicillin-resistant pneumococcus (PRSP) and the like) and multi-drug-resistant super bacteria are generated, and five types of antibiotics (beta-lactams, macrolides, quinolones, aminoglycosides and tetracyclines) which are once known as the most effective are all disabled.
Disclosure of Invention
The purpose of the invention is as follows: in order to solve the technical problems, the invention provides a broad-spectrum drug-resistant bacterium killing preparation prepared continuously by turbulence enhancement, a preparation method and application thereof. The obtained broad-spectrum drug-resistant bacteria killing preparation has the function of killing drug-resistant bacteria in a broad spectrum manner, and can be applied to various fields which are easily polluted by bacteria.
The technical scheme is as follows: in order to achieve the purpose, the invention adopts the following technical scheme:
a broad-spectrum drug-resistant bacterium killing preparation continuously prepared by turbulence reinforcement and a preparation method and application thereof comprise the following steps: respectively preparing metal salt and a weak reducing agent as raw materials into solutions, then uniformly mixing the two solutions under the combined action of impinging stream and ultrasound, carrying out redox reaction, centrifuging, washing, dissolving and dispersing a product after the reaction is finished to obtain a metal nanoparticle solution, and mixing the metal nanoparticle solution with a solution containing a broad-spectrum bactericide to obtain the metal nanoparticle.
Preferably, the method comprises the following steps:
the metal salt is selected from an Ag salt, preferably silver nitrate, Tollens reagent, or silver sulfate; the weak reducing agent is selected from sodium citrate, glucose or ascorbic acid.
The molar ratio of the metal salt to the weak reducing agent is (0.01-100): 1, and more preferably 1: (0.1 to 20), and more preferably 1: (0.5-1.5).
The metal salt and the weak reducing agent respectively take water as a solvent to prepare solutions, and the concentration of the solutions is 0.01-0.5 mol/L.
The conditions of the two solutions entering the impinging stream are 28.8-86.4rpm and 6.25-18.75 ml/min; the ultrasonic condition is 15-25kHz, 300-1200W.
After the reaction is finished, the pH value of the mixed solution is adjusted to 7-14.
The time of the oxidation-reduction reaction is 1min to 4 hours, more preferably 0.1h to 2 hours, and still more preferably 0.2h to 1 hour.
The solution containing the broad-spectrum bactericide contains the hexamethylbiguanide and the chlorhexidine acetate, the concentration of the hexamethylbiguanide and the chlorhexidine acetate is in the range of 0.01-99.9 wt%, and the mass ratio of the metal nanoparticles to the hexamethylbiguanide to the chlorhexidine acetate is 1: (1-100): (1-100), and more preferably 1 (5-100): 5-100.
The invention also provides a broad-spectrum drug-resistant bacterium killing preparation prepared by the method.
The invention finally provides the application of the broad-spectrum drug-resistant killing bacteria preparation in killing drug-resistant bacteria.
Metals and metal nanoparticles, e.g. Ag, TiO2ZnO and the like have been proved to have good broad-spectrum antibacterial ability, are not easy to cause bacteria to generate drug resistance, and have been used in the antibacterial field due to the advantages of stable physical properties, simple and convenient preparation process, no toxicity, no bacteria causing and the like. Therefore, the invention adopts metal salt and non-toxic weak reducing agent as raw materials, and the flow in the reaction environment is subjected to the synergistic action of the impinging flow and the ultrasoundThe form of the body is changed from laminar flow to turbulent flow, the micro-mixing strength and the fluid shearing force of the reaction environment are enhanced by utilizing the turbulent flow characteristic, so that the metal antibacterial nano particles with uniform appearance and small size can be rapidly and greenly prepared under the condition of not using a strong reducing agent, and the broad-spectrum drug-resistant bacterium killing preparation is prepared by matching with the hexamethyl biguanide, the chlorhexidine acetate and the like. The method adopts specific raw materials and processes to form a product with a special appearance. The method disclosed by the invention is simple and rapid to operate, green and environment-friendly, and easy for large-scale production, and the prepared metal antibacterial nanoparticles have the advantages of small particles, large specific surface area, no toxic and harmful residues and the like. In addition, the prepared sterilizing agent has the function of broad-spectrum killing of drug-resistant bacteria, and can be applied to various fields which are easily polluted by bacteria.
Has the advantages that: compared with the traditional preparation method, the preparation method has the advantages that the turbulent flow is used for enhancing the redox reaction, so that the metal antibacterial nano particles with uniform appearance, small size and no harmful reagent residue are prepared, the process is simple and easy to implement, the operation is convenient, and the large-scale production is facilitated; the average diameter of the prepared metal antibacterial nano particles is 3 +/-2 nm. The method is simple and rapid to operate, economical and environment-friendly, and the prepared broad-spectrum drug-resistant sterilization preparation has the advantages that the metal antibacterial nanoparticles are uniform in shape and small in particle size, and can realize large-scale production. The broad-spectrum drug-resistant bacterium killing preparation prepared by the method can provide safer and more efficient antibacterial performance for various fields which are easily polluted by bacteria.
Drawings
FIG. 1: is TEM spectrum of metal antibacterial nano particles prepared by the process;
FIG. 2 is a graph showing the antibacterial performance of the broad-spectrum antibiotic-killing preparation prepared by the process of the present invention.
Detailed Description
Example 1
A method for continuously preparing silver nanoparticles by turbulence enhancement comprises the following steps:
1) preparation of reaction solution: weighing a proper amount of silver nitrate and sodium citrate, and respectively adding water to dissolve the silver nitrate and the sodium citrate to 0.01 mol/L;
2) the silver nitrate solution and the sodium citrate solution (molar ratio of silver nitrate to sodium citrate in both solutions is 1:1) were passed into the impinging stream reactor at a feed rate of 57.6rpm (12.5ml/min) while being sonicated at 20kHz, 600W with an ultrasound probe.
3) The silver ions in the mixed solution are combined under the combined action of the impinging stream and the ultrasound, the reaction is carried out for 10min, silver nanoparticles are generated through oxidation-reduction reaction, and the pH value of the solution is kept between 7 and 14.
4) And centrifuging and washing the reaction product, adding deionized water, and performing ultrasonic dispersion to obtain an aqueous solution containing silver nanoparticles with uniform appearance and small size.
5) Adding the silver-containing nanoparticle aqueous solution into a mixed solution containing 0.1% of polyhexamethylene biguanide and 0.1% of chlorhexidine acetate to prepare the broad-spectrum drug-resistant antibacterial agent (wherein the mass ratio of the silver-containing nanoparticles to the polyhexamethylene biguanide to the chlorhexidine acetate is 1: 10: 10).
Example 2
A method for continuously preparing silver nanoparticles by turbulence enhancement comprises the following steps:
1) preparation of reaction solution: weighing a proper amount of silver nitrate and sodium citrate, and respectively adding water to dissolve the silver nitrate and the sodium citrate to 0.05 mol/L;
2) the silver nitrate solution and the sodium citrate solution (molar ratio of silver nitrate to sodium citrate in both solutions is 1:1.1) were passed into the impinging stream reactor at a feed rate of 57.6rpm (12.5ml/min) while being sonicated at 20kHz, 600W with an ultrasonic probe.
3) And combining the silver ions in the mixed solution under the combined action of the impinging stream and the ultrasound, reacting for 8min, generating silver nanoparticles through redox reaction, and keeping the pH value of the solution between 7 and 14.
4) And centrifuging and washing the reaction product, adding deionized water, and performing ultrasonic dispersion to obtain an aqueous solution containing silver nanoparticles with uniform appearance and small size.
5) Adding the silver-containing nanoparticle aqueous solution into a mixed solution containing 0.2% of polyhexamethylene biguanide and 0.2% of chlorhexidine acetate to prepare the broad-spectrum drug-resistant antibacterial agent (wherein the mass ratio of the silver-containing nanoparticles to the polyhexamethylene biguanide to the chlorhexidine acetate is 1: 5: 10).
Example 3
A method for continuously preparing silver nanoparticles by turbulence enhancement comprises the following steps:
1) preparation of reaction solution: weighing a proper amount of silver nitrate and sodium citrate, and respectively adding water to dissolve the silver nitrate and the sodium citrate to 0.10 mol/L.
2) The silver nitrate solution and the sodium citrate solution (molar ratio of silver nitrate to sodium citrate in both solutions is 0.9:1.) were passed into the impinging stream reactor at a feed rate of 57.6rpm (12.5ml/min) while being sonicated at 20kHz, 600W with an ultrasonic probe.
3) The silver ions in the mixed solution are combined under the combined action of the impinging stream and the ultrasound, the reaction is carried out for 10min, silver nanoparticles are generated through oxidation-reduction reaction, and the pH value of the solution is kept between 7 and 14.
4) And centrifuging and washing the reaction product, adding deionized water, and performing ultrasonic dispersion to obtain a solution containing silver nanoparticles with uniform appearance and small size.
5) Adding the silver-containing nanoparticle aqueous solution into a mixed solution containing 0.25% of polyhexamethylene biguanide and 0.25% of chlorhexidine acetate to prepare the broad-spectrum drug-resistant antibacterial agent (wherein the mass ratio of the silver-containing nanoparticles to the polyhexamethylene biguanide to the chlorhexidine acetate is 1: 10: 5).
Example 4
A method for continuously preparing silver nanoparticles by turbulence enhancement comprises the following steps:
1) preparation of reaction solution: weighing a proper amount of silver nitrate and sodium citrate, and respectively adding water to dissolve the silver nitrate and the sodium citrate to 0.20 mol/L;
2) the silver nitrate solution and the sodium citrate solution (molar ratio of silver nitrate to sodium citrate in both solutions is 1:1.1) were passed into the impinging stream reactor at a feed rate of 57.6rpm (12.5ml/min) while being sonicated at 20kHz, 600W with an ultrasonic probe.
3) The silver ions in the mixed solution are combined under the combined action of the impinging stream and the ultrasound, the reaction is carried out for 10min, silver nanoparticles are generated through oxidation-reduction reaction, and the pH value of the solution is kept between 7 and 14.
4) And centrifuging and washing the reaction product, adding deionized water, and performing ultrasonic dispersion to obtain a solution containing silver nanoparticles with uniform appearance and small size.
5) Adding the silver-containing nanoparticle aqueous solution into a mixed solution containing 0.2% of polyhexamethylene biguanide and 0.2% of chlorhexidine acetate to prepare the broad-spectrum drug-resistant antibacterial agent (wherein the mass ratio of the silver-containing nanoparticles to the polyhexamethylene biguanide to the chlorhexidine acetate is 1: 10: 20).
Example 5
A method for continuously preparing silver nanoparticles by turbulence enhancement comprises the following steps:
1) preparation of reaction solution: weighing a proper amount of silver nitrate and sodium citrate, and respectively adding water to dissolve the silver nitrate and the sodium citrate to 0.50 mol/L;
2) the silver nitrate solution and the sodium citrate solution (molar ratio of silver nitrate to sodium citrate in both solutions is 1:1.1) were passed into the impinging stream reactor at a feed rate of 57.6rpm (12.5ml/min) while being sonicated at 20kHz, 600W with an ultrasonic probe.
3) And combining the silver ions in the mixed solution under the combined action of the impinging stream and the ultrasound, reacting for 60min, and generating silver nanoparticles through oxidation-reduction reaction, wherein the pH value of the solution is kept between 7 and 14.
4) And centrifuging and washing the reaction product, adding deionized water, and performing ultrasonic dispersion to obtain a solution containing silver nanoparticles with uniform appearance and small size.
5) Adding the silver-containing nanoparticle aqueous solution into a mixed solution containing 0.15% of polyhexamethylene biguanide and 0.15% of chlorhexidine acetate to prepare the broad-spectrum drug-resistant antibacterial agent (wherein the mass ratio of the silver-containing nanoparticles to the polyhexamethylene biguanide to the chlorhexidine acetate is 1: 20: 10).
Example 6
A method for continuously preparing silver nanoparticles by turbulence enhancement comprises the following steps:
1) preparation of reaction solution: weighing a proper amount of silver nitrate and sodium citrate, and respectively adding water to dissolve the silver nitrate and the sodium citrate to 0.10 mol/L;
2) the silver nitrate solution and the sodium citrate solution (molar ratio of silver nitrate to sodium citrate in both solutions is 1:1.1) were passed into the impinging stream reactor at a feed rate of 57.6rpm (12.5ml/min) while being sonicated at 20kHz, 300W, using an ultrasonic probe.
3) And combining the silver ions in the mixed solution under the combined action of the impinging stream and the ultrasound, reacting for 90min, and generating silver nanoparticles through oxidation-reduction reaction, wherein the pH value of the solution is kept between 7 and 14.
4) And centrifuging and washing the reaction product, adding deionized water, and performing ultrasonic dispersion to obtain a solution containing silver nanoparticles with uniform appearance and small size.
5) Adding the silver-containing nanoparticle aqueous solution into a mixed solution containing 0.5% of polyhexamethylene biguanide and 0.5% of chlorhexidine acetate to prepare the broad-spectrum drug-resistant antibacterial agent (wherein the mass ratio of the silver-containing nanoparticles to the polyhexamethylene biguanide to the chlorhexidine acetate is 1: 50: 50).
Example 7
A method for continuously preparing silver nanoparticles by turbulence enhancement comprises the following steps:
1) preparation of reaction solution: weighing a proper amount of silver nitrate and sodium citrate, and respectively adding water to dissolve the silver nitrate and the sodium citrate to 0.10 mol/L;
2) the silver nitrate solution and the sodium citrate solution (molar ratio of silver nitrate to sodium citrate in both solutions is 1:1.1) were passed into the impinging stream reactor at a feed rate of 57.6rpm (12.5ml/min) while being sonicated at 20kHz, 900W with an ultrasonic probe.
3) And combining the silver ions in the mixed solution under the combined action of the impinging stream and the ultrasound, reacting for 15min, generating silver nanoparticles through redox reaction, and keeping the pH value of the solution between 7 and 14.
4) And centrifuging and washing the reaction product, adding deionized water, and performing ultrasonic dispersion to obtain a solution containing silver nanoparticles with uniform appearance and small size.
5) Adding the silver-containing nanoparticle aqueous solution into a mixed solution containing 0.2% of polyhexamethylene biguanide and 0.2% of chlorhexidine acetate to prepare the broad-spectrum drug-resistant antibacterial agent (wherein the mass ratio of the silver-containing nanoparticles to the polyhexamethylene biguanide to the chlorhexidine acetate is 1: 25: 25).
Example 8
A method for continuously preparing silver nanoparticles by turbulence enhancement comprises the following steps:
1) preparation of reaction solution: weighing a proper amount of silver nitrate and sodium citrate, and respectively adding water to dissolve the silver nitrate and the sodium citrate to 0.10 mol/L;
2) the silver nitrate solution and the sodium citrate solution (molar ratio of silver nitrate to sodium citrate in both solutions is 1:1.1) were passed into the impinging stream reactor at a feed rate of 57.6rpm (12.5ml/min) while being sonicated at 20kHz, 1200W with an ultrasonic probe.
3) The silver ions in the mixed solution are combined under the combined action of the impinging stream and the ultrasound, the reaction is carried out for 10min, silver nanoparticles are generated through oxidation-reduction reaction, and the pH value of the solution is kept between 7 and 14.
4) And centrifuging and washing the reaction product, adding deionized water, and performing ultrasonic dispersion to obtain a solution containing silver nanoparticles with uniform appearance and small size.
5) Adding the silver-containing nanoparticle aqueous solution into a mixed solution containing 0.2% of polyhexamethylene biguanide and 0.2% of chlorhexidine acetate to prepare the broad-spectrum drug-resistant antibacterial agent (wherein the mass ratio of the silver-containing nanoparticles to the polyhexamethylene biguanide to the chlorhexidine acetate is 1: 30: 30).
Example 9
A method for continuously preparing silver nanoparticles by turbulence enhancement comprises the following steps:
1) preparation of reaction solution: weighing a proper amount of silver nitrate and sodium citrate, and respectively adding water to dissolve the silver nitrate and the sodium citrate to 0.15 mol/L;
2) the silver nitrate solution and the sodium citrate solution (molar ratio of silver nitrate to sodium citrate in both solutions is 1:1.1) were passed into the impinging stream reactor at a feed rate of 28.8rpm (6.25ml/min) while being sonicated at 20kHz, 600W with an ultrasonic probe.
3) And combining the silver ions in the mixed solution under the combined action of the impinging stream and the ultrasound, reacting for 80min, and generating silver nanoparticles through oxidation-reduction reaction, wherein the pH value of the solution is kept between 7 and 14.
4) And centrifuging and washing the reaction product, adding deionized water, and performing ultrasonic dispersion to obtain a solution containing silver nanoparticles with uniform appearance and small size.
5) Adding the silver-containing nanoparticle aqueous solution into a mixed solution containing 0.5% of polyhexamethylene biguanide and 0.5% of chlorhexidine acetate to prepare the broad-spectrum drug-resistant antibacterial agent (wherein the mass ratio of the silver-containing nanoparticles to the polyhexamethylene biguanide to the chlorhexidine acetate is 1: 100: 100).
Example 10
A method for continuously preparing silver nanoparticles by turbulence enhancement comprises the following steps:
1) preparation of reaction solution: weighing a proper amount of silver nitrate and sodium citrate, and respectively adding water to dissolve the silver nitrate and the sodium citrate to 0.05 mol/L;
2) the silver nitrate solution and the sodium citrate solution (molar ratio of silver nitrate to sodium citrate in both solutions is 1:1.1) were passed into the impinging stream reactor at a feed rate of 86.4rpm (18.75ml/min) while being sonicated at 20kHz, 600W with an ultrasonic probe.
3) And combining the silver ions in the mixed solution under the combined action of the impinging stream and the ultrasound, reacting for 20min, and generating silver nanoparticles through oxidation-reduction reaction, wherein the pH value of the solution is kept between 7 and 14.
4) And centrifuging and washing the reaction product, adding deionized water, and performing ultrasonic dispersion to obtain a solution containing silver nanoparticles with uniform appearance and small size.
5) Adding the silver-containing nanoparticle aqueous solution into a mixed solution containing 0.2% of polyhexamethylene biguanide and 0.2% of chlorhexidine acetate to prepare the broad-spectrum drug-resistant antibacterial agent (wherein the mass ratio of the silver-containing nanoparticles to the polyhexamethylene biguanide to the chlorhexidine acetate is 1: 25: 50).
The silver nanoparticles prepared in the above examples were physically characterized by means of TEM and antibacterial tests. As can be seen from TEM (fig. 1), the metal nanoparticles (silver nanoparticles) prepared according to the method of the present invention are silver nanoparticles having a uniform morphology and a small size, and have an average diameter of 5 ± 3 nm. FIG. 2 is a performance diagram of the broad-spectrum pesticide-resistant bacteria preparation prepared by the process of the invention, and it can be seen that the broad-spectrum pesticide-resistant bacteria preparation prepared by the process of the invention has good antibacterial performance.
Claims (10)
1. A method for continuously preparing a broad-spectrum drug-resistant bacterium killing preparation by turbulence enhancement is characterized by comprising the following steps: respectively preparing metal salt and a weak reducing agent as raw materials into solutions, then uniformly mixing the two solutions under the combined action of impinging stream and ultrasound, carrying out redox reaction, centrifuging, washing, dissolving and dispersing a product after the reaction is finished to obtain a metal nanoparticle solution, and mixing the metal nanoparticle solution with a solution containing a broad-spectrum bactericide to obtain the metal nanoparticle.
2. The process for the construction and the turbulization continuous preparation of the broad spectrum drug-resistant bacteria killing preparation according to claim 1, wherein the metal salt is selected from Ag salt, preferably silver nitrate, Tollens reagent, or silver sulfate; the weak reducing agent is selected from sodium citrate, glucose or ascorbic acid.
3. The method for continuously preparing the broad-spectrum pesticide-resistant bacteria preparation through turbulence-strengthening according to claim 1, wherein the molar ratio of the metal salt to the weak reducing agent is (0.01-100): 1.
4. The method for continuously preparing the broad-spectrum drug-resistant bacteria preparation through turbulence-enhanced process according to claim 1, wherein the metal salt and the weak reducing agent are respectively prepared into a solution by using water as a solvent, and the concentration of the metal salt and the weak reducing agent is 0.01-5 mol/L.
5. The method for continuously preparing the broad spectrum pesticide-resistant bacteria preparation through turbulence-intensification as claimed in claim 1, wherein the conditions of the two solutions entering the impinging stream are 28.8-86.4rpm, 6.25-18.75 ml/min; the ultrasonic condition is 15-25kHz, 300-1200W.
6. The method for continuously preparing the broad-spectrum drug-resistant bacteria preparation through turbulence-enhanced process according to claim 1, wherein the reaction is carried out while maintaining the pH value of the mixed solution at 7-14.
7. The method for continuously preparing the broad-spectrum drug-resistant bacteria killing preparation through turbulence enhancement according to claim 1, wherein the time of the oxidation-reduction reaction is 1min-4 h.
8. The method for continuously preparing the broad spectrum drug-resistant bacteria killing preparation through turbulence-strengthening according to claim 1, wherein the solution containing the broad spectrum bactericide contains the poly-hexamethyl biguanide and the chlorhexidine acetate, the concentration of the poly-hexamethyl biguanide and the chlorhexidine acetate is in the range of 0.01-99.9 wt%, and the mass ratio of the metal nanoparticles to the poly-hexamethyl biguanide to the chlorhexidine acetate is 1: 1-100: 1-100.
9. A broad spectrum drug-fast-killing bacteria preparation prepared by the method of any one of claims 1-8.
10. The use of the broad spectrum drug resistant microbe killing formulation of claim 9 in killing drug resistant bacteria.
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