CN105732842B - A kind of high bioactivity chitin and preparation method thereof - Google Patents
A kind of high bioactivity chitin and preparation method thereof Download PDFInfo
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- CN105732842B CN105732842B CN201610099240.0A CN201610099240A CN105732842B CN 105732842 B CN105732842 B CN 105732842B CN 201610099240 A CN201610099240 A CN 201610099240A CN 105732842 B CN105732842 B CN 105732842B
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- chitin
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
- C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
- C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The invention provides a kind of high bioactivity chitin and preparation method thereof, the high bioactivity chitin is prepared using following steps：Organism containing β chitins is scrubbed, forms powder after dry, crushing；Powder after crushing is poured into stirring in the mixed ammonium/alkali solutions that concentration is 0.5M~10M and carries out the de- albumen of alkali process；Dilute hydrochloric acid solution is added to step S2 by the Deproteinated product of alkali process, regulation pH value to 5 ~ 7, being stood at 20 ~ 30 DEG C, inorganic salts is removed, is filtrated to get β chitins.There is strong molecular weight height, adsorption capacity, the adsorptivity that amino reserved is high, excellent and the characteristic such as moisture absorbability and moisture retentivity and the deacetylation of β chitins of superelevation using the chitin obtained by technical scheme, so that product has very high bioactivity, technical field of biological material can be widely applied to.
Description
Technical field
The invention belongs to technical field of biological material, more particularly to a kind of high bioactivity chitin and preparation method thereof.
Background technology
Presently commercially available native biopolymer material chitin is isolated from shrimp and crab shells, abundance.It is existing at present a variety of
Method chitin extraction from shrimp and crab shells, including take chemical treatment and mechanical lapping-ultrasound-centrifugal process, heterogeneous synthesis side
The methods of method, freeze-thaw.The composition of shrimp and crab shells is mainly chitin, torpescence on these chitin chemistries, is not sent out with body fluid
Changing, it is nontoxic to can not organize foreign body reaction, there is antithrombotic, high temperature-resistant disinfected.The crust prepared by chitin
Element and its derivative have the characteristics such as extraordinary biocompatibility, biodegradability, anti-inflammation, extensively should at present
For artificial cornea support, tissue engineering comea, tissue engineering bracket, the biomedical neck such as artificial vitreous and selfreparing
Domain.
Include chemical treatment and mechanical lapping-ultrasound-centrifugal process, heterogeneous synthetic method, freeze-thaw etc. at present
Method chitin extraction from shrimp and crab shells, but more or less there are problems that：
1st, mechanical lapping-ultrasound-centrifugal process prepares chitin yield is relatively low；
2nd, multi-phases process is alkalized using high-concentration alkali liquor, and molecular weight product degraded is serious, and environmental pollution is big；
3rd, multi-phases process is easily destroyed state of aggregation mechanism in dissolving and process of setting, obtained chitin mechanics
Performance is weak and crisp, limits application of the chitin as membrane material；
4th, freeze-thaw method prepares chitin solution and takes, consumes energy, and is not suitable for large-scale production；
5th, the amino with high activity can be dissolved in diluted acid, chemically reacted under given conditions, and generation has
The derivative of different performance；
6th, high deacetylized α-chitin has higher amino reserved, and these amino can be under certain condition by proton
The natural polymer changed and make chitin turn into positively charged, participate in the anionic compounds such as absorption heavy metal ion, aliphatic acid.But
Our research indicate that when the deacetylation of α-chitin is more than 90%, because strand tends to be relatively regular, crystallinity carries
Height causes to reduce in the amino content of solid-state chitin surface and amorphous regions, and adsorption effect reduces suddenly.
The content of the invention
For above technical problem, the invention discloses a kind of high bioactivity chitin and preparation method thereof, obtain
Chitin has strong molecular weight height, adsorption capacity, the adsorptivity that amino reserved is high, excellent and moisture absorbability and moisture retentivity and superelevation
The characteristics such as the deacetylation of β-chitin so that product has very high bioactivity, can be removed extensively using antimycotic antiseptic
Smelly dose, medical operation line, wound auxiliary material, food preservative, disease resistance breeding feed, the neck such as NMF and man-made support material
Domain.
On the other hand, the technical solution adopted by the present invention is：
A kind of high bioactivity chitin, the high bioactivity chitin are β-chitin, and the β-chitin uses
Following steps are prepared：
Step S1：Organism containing β-chitin is washed, dries, be ground into powder；
Step S2：Powder after crushing is poured into stirring in the mixed ammonium/alkali solutions that concentration is 0.5M~10M and carries out alkali process
De- albumen, and make the volume ratio of solid and liquid be 1: 5~1: 50, mixing speed be 50~3000rpm, temperature for 30 DEG C~
150 DEG C, 0.5~24h of mixing time,；Wherein, the component and its percentage by weight that the mixed ammonium/alkali solutions include be：Hydroxide
Sodium 2-20wt%, potassium hydroxide 2-20wt%, polyethylene glycol 0-5wt%, urea 2-10wt%, surplus are water；
Step S3：Dilute hydrochloric acid solution is added into step S2 to pass through in the Deproteinated product of alkali process, regulation pH value to 5~
7, stood at 20~30 DEG C, acid-base neutralization removes inorganic salts, is filtrated to get β-chitin.Preferably, it is quiet at 20~30 DEG C
Put 8~16h；It is further preferred that stand 12h at 20~30 DEG C.
Using this technical scheme, the deacetylation and molecular weight of obtained β-chitin are high, and its deacetylation is not less than
95%, amino content is not less than 9.5%, and in the case where guarantee is high deacetylized, keeps high amino content, do not influence high-activity ommonia
Base；Other molecular weight can reach more than 150,000, and high can be up to 350,000, the significantly larger than molecule of the chitin of prior art
Amount；And as the raising of molecular weight, its adsorption capacity are also improved.
As a further improvement on the present invention, in step S3, regulation pH value to 6.
As a further improvement on the present invention, the organism containing β-chitin is squid cartilage.
As a further improvement on the present invention, in addition to step S4：β-chitin is rinsed repeatedly with deionized water to neutrality,
Drying and grinding obtains the pure β-chitin product with high bioactivity.
As a further improvement on the present invention, in the step S2, inorganic matter pore-foaming agent is additionally added in stirring and is stirred
Mix.
As a further improvement on the present invention, the inorganic matter pore-foaming agent is NaCl, KCl, CaCl2At least one of,
The dosage of the inorganic matter pore-foaming agent is 0.05-1wt%.
As a further improvement on the present invention, in step S1, the powder is 10 μm~2mm fibrous powder.
The invention also discloses a kind of preparation method of high bioactivity chitin as described above, comprise the following steps：
Step S1：By the organism washing containing β-chitin, the fibrous powder dried, be ground into 10 μm~2mm；It is excellent
Choosing, the organism containing β-chitin is squid cartilage；
Step S2：Powder after crushing is poured into stirring in the mixed ammonium/alkali solutions that concentration is 0.5M~10M and carries out alkali process
De- albumen, and make the volume ratio of solid and liquid be 1: 5~1: 50, mixing speed be 50~3000rpm, temperature for 30 DEG C~
150 DEG C, 0.5~24h of mixing time,；Wherein, the component and its percentage by weight that the mixed ammonium/alkali solutions include be：Hydroxide
Sodium 2-20wt%, potassium hydroxide 2-20wt%, polyethylene glycol 0-5wt%, urea 2-10wt%, surplus are water；And add inorganic
Thing pore-foaming agent is stirred；Wherein, the inorganic matter pore-foaming agent is NaCl, KCl, CaCl2At least one of, the inorganic matter
The dosage of pore-foaming agent is 0.05-1wt%；
Step S3：Dilute hydrochloric acid solution is added into step S2 to pass through in the Deproteinated product of alkali process, regulation pH value to 5~
7,8~16h is stood at 20~30 DEG C, acid-base neutralization removes inorganic salts, is filtrated to get β-chitin；Preferably, 12h is stood.
Step S4：β-chitin is rinsed repeatedly with deionized water to neutrality, drying and grinding obtains having high bioactivity
Pure β-chitin product.
Compared with prior art, beneficial effects of the present invention are：
First, using technical scheme, the deacetylation and molecular weight of obtained β-chitin are high, and its is deacetylated
Degree is not less than 95%, and amino content is not less than 9.5%, and in the case where guarantee is high deacetylized, keeps high amino content, do not influence
High activity amino；Other molecular weight can reach more than 150,000, and high can be up to 350,000, significantly larger than presently commercially available crust
The molecular weight (100,000 or so) of element；And as the raising of molecular weight, its adsorption capacity are also improved.
Second, using technical scheme, by adding the materials such as urea, polyethylene glycol, help to increase crust
The stability of element, lifts its physical property；Reaction is gentle, small to chitin molecule destruction, is adjusted when acid treatment removes inorganic salts
PH value is 5~7 or so, and amino reserved is high so that product has very high bioactivity；When the deacetylation of β-chitin
When up to 100%, had an impact all without to high activity amino；More hollow structures are formed by adding perforating agent, product is existed
Can preferably it be realized with other compositions compound.
3rd, technical scheme, raw material sources are extensive, production process is simple, clean environment firendly, cost are cheap, fit
Close industrialized production；The chitin of obtained high bioactivity has water-soluble, excellent adsorptivity and moisture absorbability and moisture retentivity, Ke Yiguang
General application antimycotic antiseptic deodorant, medical operation line, wound auxiliary material, food preservative, disease resistance breeding feed, NMF and people
The fields such as work timbering material.
Brief description of the drawings
Fig. 1 is that an embodiment of the present invention Escherichia coli aqueous suspension nutrient solution is aobvious before high bioactivity chitin is added
Micro mirror figure.
It is 3 small after the high bioactivity chitin of embodiment 1 that Fig. 2 is that the present invention adds in Escherichia coli aqueous suspension nutrient solution
When microscope figure.
It is 3 small after the high bioactivity chitin of embodiment 2 that Fig. 3 is that the present invention adds in Escherichia coli aqueous suspension nutrient solution
When microscope figure.
Fig. 4 is that the present invention adds after the chitin of comparative example 13 hours aobvious in Escherichia coli aqueous suspension nutrient solution
Micro mirror figure.
Embodiment
The preferably embodiment of the present invention is described in further detail below.
Embodiment 1
A kind of high bioactivity chitin, the high bioactivity chitin are β-chitin, and the β-chitin takes off
Acetyl degree is more than 95%, and amino content is more than 9.5%；It is prepared using following steps：
Step S1：By the fibrous powder that is scrubbed, dry, being ground into 10 μm~2mm of the squid cartilage containing β-chitin
End；
Step S2：Alkali process takes off albumen：
Powder after step S1 is crushed pours into stirring in the mixed ammonium/alkali solutions that concentration is 0.5M and carries out the de- albumen of alkali process,
And making the volume ratio of solid and liquid be 1: 50, mixing speed 1000rpm, temperature is 80 DEG C, mixing time 18h；Wherein, institute
State component that mixed ammonium/alkali solutions include and its percentage by weight is：Sodium hydroxide 2wt%, potassium hydroxide 2wt%, polyethylene glycol
1wt%, urea 2wt%, surplus are water；And add inorganic matter pore-foaming agent and be stirred, to form loose structure；Wherein, it is described
Inorganic matter pore-foaming agent is NaCl, and the dosage of the NaCl is 1wt%；
Step S3：Inorganic salts are removed through acid treatment：
Dilute hydrochloric acid solution is added in the above-mentioned process Deproteinated product of alkali process and adjusts pH value to 5.0, at 25 DEG C
Stand 12h and remove inorganic salts, be filtrated to get β-chitin；
Step S4：β-chitin is rinsed repeatedly with deionized water to neutrality, drying and grinding obtains having high bioactivity
Pure β-chitin product.
Embodiment 2
A kind of high bioactivity chitin, the high bioactivity chitin are β-chitin, and the β-chitin uses
Following steps are prepared：
Step S1：By the squid cartilage washing containing β-chitin, the fibrous powder dried, be ground into 10 μm~2mm；
Preferably, the organism containing β-chitin is squid cartilage；
Step S2：Powder after crushing is poured into stirring in the mixed ammonium/alkali solutions that concentration is 10M and carries out the de- albumen of alkali process,
And make the volume ratio of solid and liquid be 1: 5, mixing speed 3000rpm, temperature is 150 DEG C, mixing time 0.5h,；Wherein,
The component and its percentage by weight that the mixed ammonium/alkali solutions include be：Sodium hydroxide 20wt%, potassium hydroxide 20wt%, poly- second two
Alcohol 5wt%, urea 10wt%, surplus are water；And add inorganic matter pore-foaming agent and be stirred；Wherein, the inorganic matter pore-foaming agent
For CaCl2, the dosage of the inorganic matter pore-foaming agent is 0.05wt%；
Step S3：Dilute hydrochloric acid solution is added to step S2 by the Deproteinated product of alkali process, regulation pH value to
6.0,8h is stood at 20 DEG C, inorganic salts is removed, is filtrated to get β-chitin；
Step S4：β-chitin is rinsed repeatedly with deionized water to neutrality, drying and grinding obtains having high bioactivity
Pure β-chitin product.
Embodiment 3
A kind of high bioactivity chitin, the high bioactivity chitin are β-chitin, and the β-chitin uses
Following steps are prepared：
Step S1：By the squid cartilage washing containing β-chitin, the fibrous powder dried, be ground into 10 μm~2mm；
Preferably, the organism containing β-chitin is squid cartilage；
Step S2：Powder after crushing is poured into stirring in the mixed ammonium/alkali solutions that concentration is 5M and carries out the de- albumen of alkali process,
And make the volume ratio of solid and liquid be 1: 20, mixing speed 500rpm, temperature is 50 DEG C, mixing time 24h,；Wherein, institute
State component that mixed ammonium/alkali solutions include and its percentage by weight is：Sodium hydroxide 12wt%, potassium hydroxide 15wt%, polyethylene glycol
3wt%, urea 4wt%, surplus are water；And add inorganic matter pore-foaming agent and be stirred；Wherein, the inorganic matter pore-foaming agent is
KCl, the dosage of the inorganic matter pore-foaming agent is 0.6wt%；
Step S3：Dilute hydrochloric acid solution is added to step S2 by the Deproteinated product of alkali process, regulation pH value to
6.5,16h is stood at 30 DEG C, inorganic salts is removed, is filtrated to get β-chitin；
Step S4：β-chitin is rinsed repeatedly with deionized water to neutrality, drying and grinding obtains having high bioactivity
Pure β-chitin product.
Comparative example 1
A kind of β-chitin, it uses following steps to be prepared：
Step S1：By the squid cartilage washing containing β-chitin, the fibrous powder dried, be ground into 10 μm~2mm；
Preferably, the organism containing β-chitin is squid cartilage；
Step S2：Powder after crushing is poured into stirring in the mixed ammonium/alkali solutions that concentration is 1M and carries out the de- albumen of alkali process,
And make the volume ratio of solid and liquid be 1: 10, mixing speed 2000rpm, temperature is 30 DEG C, mixing time 24h,；Wherein,
The component and its percentage by weight that the mixed ammonium/alkali solutions include be：Sodium hydroxide 12wt%, potassium hydroxide 15wt%, poly- second two
Alcohol 3wt%, urea 4wt%, surplus are water；And add inorganic matter pore-foaming agent and be stirred；Wherein, the inorganic matter pore-foaming agent is
KCl, the dosage of the inorganic matter pore-foaming agent is 0.6wt%；
Step S3：Dilute hydrochloric acid solution is added to step S2 by the Deproteinated product of alkali process, regulation pH value to
7.0,16h is stood at 30 DEG C, inorganic salts is removed, is filtrated to get β-chitin；
Step S4：β-chitin is rinsed repeatedly with deionized water to neutrality, drying and grinding obtains having high bioactivity
Pure β-chitin product.
Embodiment 1~3 and comparative example 1 are detected, as a result as shown in table 1.
The performance test results table of the embodiment 1~3 of table 1 and comparative example 1
Comparative result by table 1 is visible, and the β obtained using technical scheme-chitin molecule amount is much high
In comparative example 1, particularly in pH=6.0, the molecular weight of obtained β-chitin may be up to 350,000, and embodiment 1
In the case of~3 deacetylations height and 95%, amino content can also reach more than 9.5%, and 60 minutes to resistant micro-organism
Sterilizing rate can reach 100%, stronger to the agglomeration of Escherichia coli higher than the 90% of comparative example 1.
The chitin that embodiment is obtained is added to Escherichia coli aqueous suspension nutrient solution, and Fig. 1 is the microscope figure before addition,
Fig. 2 is microscope figure of the addition 1 high activity chitin of embodiment after 3 hours, and Fig. 3 is small for the addition high activity chitin 3 of embodiment 2
When after microscope figure, Fig. 4 is microscope figure of the β-chitin after 3 hours of addition comparative example 1.Contrasted by Fig. 1~Fig. 4
It can be seen that adding, high activity chitin prepared by the present invention is obvious to Escherichia coli agglomerating effect, the production that especially prepared by embodiment 2
Product agglomerating effect is optimal, and compared with comparative example 1, high activity chitin prepared by the present invention has stronger suction-operated.
Above content is to combine specific preferred embodiment further description made for the present invention, it is impossible to is assert
The specific implementation of the present invention is confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of not departing from present inventive concept, some simple deduction or replace can also be made, should all be considered as belonging to the present invention's
Protection domain.
Claims (8)
- A kind of 1. high bioactivity chitin, it is characterised in that：The high bioactivity chitin is β-chitin, its use with Lower step is prepared：Step S1：Organism containing β-chitin is washed, dries, be ground into powder；Step S2：Powder after crushing is poured into stirring in the mixed ammonium/alkali solutions that alkaline matter concentration is 0.5M~10M and carries out alkali The de- albumen of processing, and make the volume ratio of solid and liquid be 1: 5~1: 50, mixing speed is 50~3000rpm, and temperature is 30 DEG C ~150 DEG C, 0.5~24h of mixing time；Wherein, the component and its percentage by weight that the mixed ammonium/alkali solutions include be：Hydroxide Sodium 2-20wt%, potassium hydroxide 2-20wt%, polyethylene glycol 0-5wt%, urea 2-10wt%, surplus are water；Step S3：The dilute hydrochloric acid solution that concentration is 0.05-1mol/L is added to step S2 and passes through the Deproteinated product of alkali process In, regulation pH value stands at 20 ~ 30 DEG C to 5 ~ 7, is filtrated to get β-chitin.
- 2. high bioactivity chitin according to claim 1, it is characterised in that：In step S3, regulation pH value to 6.
- 3. high bioactivity chitin according to claim 1 or 2, it is characterised in that：The life containing β-chitin Object is squid cartilage.
- 4. high bioactivity chitin according to claim 3, it is characterised in that also including step S4：Use deionized water β-chitin is rinsed repeatedly to neutrality, and drying and grinding obtains the pure β-chitin product with high bioactivity.
- 5. high bioactivity chitin according to claim 4, it is characterised in that：In the step S2, in stirring also Inorganic matter pore-foaming agent is added to be stirred.
- 6. high bioactivity chitin according to claim 5, it is characterised in that：The inorganic matter pore-foaming agent be NaCl, KCl、CaCl2At least one of, the dosage of the inorganic matter pore-foaming agent is 0.05-1wt%.
- 7. high bioactivity chitin according to claim 1 or 2, it is characterised in that：In step S1, the powder is 10 μm~2mm fibrous powder.
- 8. a kind of preparation method of high bioactivity chitin as claimed in claim 1, it is characterised in that including following step Suddenly：Step S1：By the organism washing containing β-chitin, the fibrous powder dried, be ground into 10 μm~2mm；Step S2：Powder after crushing is poured into stirring in the mixed ammonium/alkali solutions that concentration is 0.5M~10M and carries out the de- egg of alkali process In vain, and the volume ratio of solid and liquid is made to be 1: 5~1: 50, mixing speed is 50~3000rpm, and temperature is 30 DEG C~150 DEG C, 0.5~24h of mixing time；Wherein, the component and its percentage by weight that the mixed ammonium/alkali solutions include be：Sodium hydroxide 2- 20wt%, potassium hydroxide 2-20wt%, polyethylene glycol 0-5wt%, urea 2-10wt%, surplus are water；And add inorganic matter pore-foaming agent It is stirred；Wherein, the inorganic matter pore-foaming agent is NaCl, KCl, CaCl2At least one of, the inorganic matter pore-foaming agent Dosage is 0.05-1wt%；Step S3：The dilute hydrochloric acid solution that concentration is 0.05-1mol/L is added to step S2 and passes through the Deproteinated product of alkali process In, regulation pH value stands at 20 ~ 30 DEG C to 5 ~ 7, is filtrated to get β-chitin；Step S4：β-chitin is rinsed repeatedly with deionized water to neutrality, drying and grinding obtain the pure β with high bioactivity- Chitin product.
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| FR3075204B1 (en) | 2017-12-15 | 2020-11-20 | Ynsect | CHITINE AND PROCESS FOR OBTAINING CHITINE AND / OR CHITOSAN BY CHEMICAL MEANS |
| CN110452316A (en) * | 2019-09-26 | 2019-11-15 | 浙江海洋大学 | A method of extracting β-chitin from squid cartilage |
| CN112301080A (en) * | 2020-12-22 | 2021-02-02 | 荣成广润水产食品有限公司 | Preparation method of squid cartilage beta-chitosan oligosaccharide |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1408729A (en) * | 2002-09-19 | 2003-04-09 | 青岛海洋大学 | Process for preparing non-derivative low deacetylation water soluble chitin |
| CN102372795A (en) * | 2010-08-25 | 2012-03-14 | 浙江科技学院 | Method for preparing beta-chitin by utilizing squid theca |
| CN102875694A (en) * | 2011-07-15 | 2013-01-16 | 浙江科技学院 | Method for preparing water soluble chitosan oligosaccharide from squid sheath and bioactivity of chitosan oligosaccharide |
| CN104693318A (en) * | 2015-03-24 | 2015-06-10 | 中国科学院海洋研究所 | Beta-chitosan preparation method |
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2016
- 2016-02-23 CN CN201610099240.0A patent/CN105732842B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1408729A (en) * | 2002-09-19 | 2003-04-09 | 青岛海洋大学 | Process for preparing non-derivative low deacetylation water soluble chitin |
| CN102372795A (en) * | 2010-08-25 | 2012-03-14 | 浙江科技学院 | Method for preparing beta-chitin by utilizing squid theca |
| CN102875694A (en) * | 2011-07-15 | 2013-01-16 | 浙江科技学院 | Method for preparing water soluble chitosan oligosaccharide from squid sheath and bioactivity of chitosan oligosaccharide |
| CN104693318A (en) * | 2015-03-24 | 2015-06-10 | 中国科学院海洋研究所 | Beta-chitosan preparation method |
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