CN111837955A - Culture medium and method for promoting germination of rhubarb seeds for Enshi medicine - Google Patents
Culture medium and method for promoting germination of rhubarb seeds for Enshi medicine Download PDFInfo
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- 241000219061 Rheum Species 0.000 title claims abstract description 56
- 235000009411 Rheum rhabarbarum Nutrition 0.000 title claims abstract description 55
- 230000035784 germination Effects 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 40
- 230000001737 promoting effect Effects 0.000 title claims abstract description 29
- 239000001963 growth medium Substances 0.000 title claims abstract description 16
- 239000003814 drug Substances 0.000 title description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 43
- 229930192334 Auxin Natural products 0.000 claims abstract description 33
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims abstract description 33
- 239000002363 auxin Substances 0.000 claims abstract description 33
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims abstract description 33
- 240000004980 Rheum officinale Species 0.000 claims abstract description 27
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000004062 cytokinin Substances 0.000 claims abstract description 27
- 239000008367 deionised water Substances 0.000 claims abstract description 27
- 239000012153 distilled water Substances 0.000 claims abstract description 16
- 238000012258 culturing Methods 0.000 claims abstract description 13
- 230000001954 sterilising effect Effects 0.000 claims abstract description 11
- 230000007226 seed germination Effects 0.000 claims abstract description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 23
- 102000016938 Catalase Human genes 0.000 claims description 9
- 108010053835 Catalase Proteins 0.000 claims description 9
- 239000002689 soil Substances 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 abstract description 22
- 230000012010 growth Effects 0.000 abstract description 7
- 238000000354 decomposition reaction Methods 0.000 abstract description 5
- 231100000614 poison Toxicity 0.000 abstract description 4
- 239000003440 toxic substance Substances 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 2
- 241000758993 Equisetidae Species 0.000 description 12
- 230000000694 effects Effects 0.000 description 10
- 230000004083 survival effect Effects 0.000 description 6
- 238000010998 test method Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 230000007547 defect Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 231100000419 toxicity Toxicity 0.000 description 3
- 230000001988 toxicity Effects 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 208000004998 Abdominal Pain Diseases 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- 206010023126 Jaundice Diseases 0.000 description 1
- 241000219050 Polygonaceae Species 0.000 description 1
- 235000008081 Rheum officinale Nutrition 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- PYKYMHQGRFAEBM-UHFFFAOYSA-N anthraquinone Natural products CCC(=O)c1c(O)c2C(=O)C3C(C=CC=C3O)C(=O)c2cc1CC(=O)OC PYKYMHQGRFAEBM-UHFFFAOYSA-N 0.000 description 1
- -1 anthraquinone compounds Chemical class 0.000 description 1
- 230000006851 antioxidant defense Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical compound [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
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- 239000008107 starch Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
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- 239000000126 substance Substances 0.000 description 1
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- 208000024891 symptom Diseases 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- Botany (AREA)
- Soil Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of traditional Chinese medicinal materials, and discloses a culture medium and a method for promoting germination of rhubarb seeds for Enshi, wherein the culture medium for promoting germination of rhubarb seeds for Enshi comprises 15-25 parts of auxin, 20-40 parts of cytokinin, 40-60 parts of 6-benzylaminopurine and 850-950 parts of deionized or distilled water. The method for promoting seed germination of rhubarb for enrichments comprises the following steps: sterilizing the culture dish and filter paper at high temperature and high pressure; the method comprises the following steps: adding culture solution with different components to the filter paper until the filter paper is completely soaked; culturing at constant temperature, illuminating, and culturing in dark; counting germination data of medicinal rhubarb seeds; counting root length data; and (5) carrying out statistics on the physiological index data. Experiments of the invention show that under the condition of the component, the decomposition of toxic substances such as hydrogen peroxide and the like is facilitated, the medicinal rhubarb seeds are protected from being poisoned by the hydrogen peroxide, and the germination and growth of the medicinal rhubarb seeds are promoted.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicinal materials, and particularly relates to a culture medium and a method for promoting germination of rhubarb seeds for enrichments.
Background
Rhubarb (Rheum officinale Baill) is a plant of Rheum genus of Polygonaceae family, and is used as a medicine by drying roots and rhizomes, and is also called as "horseshoe rhubarb" because the center of the rhizome shrinks after being dried and becomes a horseshoe shape. The method has the advantages of cold climate, diffuse cloud and rich soil in high mountainous areas of Enshi, is most suitable for growth and propagation of medicinal rhubarb, and has good quality and high yield, and the active ingredient is anthraquinone compounds. The medicinal rhubarb is bitter in taste and cold in nature, has the functions of purging heat and promoting bowel movement, cooling blood and removing toxicity, and removing blood stasis and stimulating the menstrual flow, and is mainly used for treating excess heat constipation, abdominal pain due to stagnation, dysenteric diarrhea, damp-heat jaundice and other symptoms.
The medicinal rhubarb is divided into two propagation modes of root-stem bud propagation and seed propagation, wherein the root-stem bud propagation method has higher survival rate, but the number of the root-stem buds germinated on the root part of the mature medicinal rhubarb can only meet the requirement of the planting of a small amount of limited medicinal rhubarb, if the large-scale planting production is required, the root-stem bud propagation method can not meet the production requirement, and the seed propagation method is required to meet the large-scale planting production.
The conventional seed seedling raising method has the defects of low seed germination rate, low seedling survival rate, long seedling raising period, slow plant growth, high seedling raising cost and the like.
Therefore, in order to germinate the rhubarb horsetails in a predetermined period of time and increase the yield of rhubarb horsetails, it is necessary to find a method for promoting germination of rhubarb horsetails for engendering.
Through the above analysis, the problems and defects of the prior art are as follows:
(1) in the germination of the rhubarb seeds in the prior art, the decomposition of toxic substances such as hydrogen peroxide and the like is not facilitated, the rhubarb seeds cannot be subjected to the toxicity of the hydrogen peroxide, and the germination rate of the rhubarb seeds is low.
(2) In the germination of the rhubarb seeds for medical use in the prior art, gibberellin and other single hormones are mainly adopted for treatment, which is not the same as the component treatment method adopted in the invention, and the effect is good.
The difficulty in solving the above problems and defects is: the invention adopts a method of different hormone component ratios to find a culture medium and a method suitable for germination of the rhubarb for Enshi medicine.
The significance of solving the problems and the defects is as follows: the medicinal rhubarb seeds germinated by the method have high germination rate, high survival rate of seedlings, short seedling culture period, quick plant growth and low seedling culture cost, and can realize and meet the requirement of large-area planting of medicinal rhubarb.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a culture medium and a method for promoting germination of rhubarb seeds for Enshi drugs.
The culture medium for promoting the germination of the rhubarb seeds for Enshi consists of 15-25 parts by mass of auxin, 20-40 parts by mass of cytokinin, 40-60 parts by mass of 6-benzylaminopurine and 850 parts by mass of deionized or distilled water.
The invention also aims to provide a method for promoting the germination of rhubarb seeds for enrichments by using the culture medium for promoting the germination of rhubarb seeds for enrichments, which comprises the following steps:
step one, performing high-temperature and high-pressure sterilization treatment on a culture dish and filter paper;
step two, component setting:
adding culture solution with different components on the filter paper until the filter paper is completely soaked;
step four, culturing at constant temperature, illuminating, and culturing in darkness;
step five, counting germination data of the medicinal rhubarb seeds;
sixthly, counting root length data;
and seventhly, counting the physiological index data.
Further, the step one or the following steps are adopted:
nutrient soil: and (5) performing high-temperature and high-pressure sterilization treatment.
Further, in the second step, the component setting comprises:
a: 950 parts of deionized water +50 parts of 6-benzylaminopurine;
b: 980 parts of deionized water +20 parts of auxin;
c: 800 parts of deionized water, 40 parts of auxin, 60 parts of cytokinin and 100 parts of 6-benzylaminopurine;
d: 950 parts of deionized water +10 parts of auxin +15 parts of cytokinin +25 parts of 6-benzylaminopurine;
e: 900 parts of deionized water, 20 parts of auxin, 30 parts of cytokinin and 50 parts of 6-benzylaminopurine.
Further, in the second step, the component setting further includes:
a) the method comprises the following steps 950 parts of distilled water +50 parts of 6-benzylaminopurine;
b) the method comprises the following steps 980 parts of distilled water +20 parts of auxin;
c) the method comprises the following steps 950 parts of distilled water +10 parts of auxin +15 parts of cytokinin +25 parts of 6-benzylaminopurine;
d) the method comprises the following steps 900 parts of distilled water, 20 parts of auxin, 30 parts of cytokinin and 50 parts of 6-benzylaminopurine;
e: 900 parts of deionized water, 20 parts of auxin, 30 parts of cytokinin and 50 parts of 6-benzylaminopurine.
Further, after the components are set, the different component combinations are added into the flowerpot for the first soaking, and the corresponding component combinations are supplemented every 5 days in the later period.
Further, the fourth step specifically comprises: incubation at 23 ℃ in a constant temperature incubator, 8 hours light, 16 hours dark.
Further, the sixth step further comprises: the root length of the medicinal rhubarb seeds germinated from the 4 th day to the 8 th day is counted respectively, and the test is repeated for four times.
Further, the steps further comprise: and respectively counting the physiological indexes of soluble sugar, catalase and the like of the medicinal rhubarb seeds germinating from the 4 th to the 8 th days, and repeating the test for three times.
By combining all the technical schemes, the invention has the advantages and positive effects that:
experiments of the invention show that under the condition of the component, the decomposition of toxic substances such as hydrogen peroxide and the like is facilitated, the medicinal rhubarb seeds are protected from being poisoned by the hydrogen peroxide, and the germination and growth of the medicinal rhubarb seeds are promoted.
(1) The effect of claim 1. Direct effect + Overall Effect
(2) From the effect of claim 2.
(3) Technical effect or experimental effect of comparison.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
FIG. 1 is a flow chart of a method for promoting germination of rhubarb seeds for enrichments, which is provided by the embodiment of the invention.
FIG. 2 is a graph showing the results of statistics of germination of rhubarb seeds in the test method provided by the embodiment of the invention.
FIG. 3 is a graph showing the results of the germination of rhubarb seeds counted in the second test method provided by the embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a culture medium and a method for promoting germination of rhubarb seeds for Enshi medicine, and the invention is described in detail with reference to the attached drawings.
The culture medium for promoting germination of rhubarb seeds for Enshi medicine provided by the invention comprises 15-25 parts of auxin, 20-40 parts of cytokinin, 40-60 parts of 6-benzylaminopurine and 850 parts of deionized or distilled water.
As shown in figure 1, the invention provides a method for promoting germination of rhubarb seeds for enrichments, which comprises the following steps:
s101, performing high-temperature and high-pressure sterilization treatment on the culture dish and the filter paper.
And S102, setting components.
S103, adding culture solution with different components to the filter paper until the filter paper is completely soaked.
S104, culturing at constant temperature, illuminating, and culturing in darkness.
And S105, counting germination data of the medicinal rhubarb seeds.
And S106, counting the root length data.
And S107, counting the physiological index data.
The invention is further described below in connection with specific tests.
First, test materials
The medicinal rhubarb seeds are collected in a medicinal plant garden test base in Enshihua.
Second, test method
The first test method comprises the following steps:
1. sterilizing the culture dish and filter paper at high temperature and high pressure;
2. the components are set as follows:
a: 950 parts of deionized water and 50 parts of 6-benzylaminopurine
B: 980 parts of deionized water and 20 parts of auxin
C: 800 parts of deionized water, 40 parts of auxin, 60 parts of cytokinin and 100 parts of 6-benzylaminopurine
D: 950 parts of deionized water, 10 parts of auxin, 15 parts of cytokinin and 25 parts of 6-benzylaminopurine
E: 900 parts of deionized water, 20 parts of auxin, 30 parts of cytokinin and 50 parts of 6-benzylaminopurine
3. Adding culture solution with different components to the filter paper until the filter paper is completely soaked;
4. culturing in a constant temperature incubator at 23 ℃ for 8 hours in light and 16 hours in darkness;
5. observing and counting the germination condition of the rhubarb seeds.
The results are shown in FIG. 2: at day 4, the medicinal rhubarb seeds of component a began to sprout roots, while the medicinal rhubarb seeds of component B, component C, component D and component E had already germinated longer roots, with the root of component E being the longest. The germinated roots of the rhubarb horsetails showed an overall increasing trend with component E being most pronounced with cotyledons of the rhubarb horsetails already extending through the seed coat at day 7 and fully developed, and with leaves appearing dark green and robust at day 8.
6. Counting root length data: the root length of the medicinal rhubarb seeds germinated from the 4 th day to the 8 th day is counted respectively, and the test is repeated for four times.
As can be seen from the above table, the root length of the rhubarb horsetails germinating from day 4 to day 8 shows a gradual lengthening trend under different composition conditions, wherein under the composition E, the germination and growth of the rhubarb horsetails are more facilitated.
7. And (3) physiological index data statistics: and respectively counting the physiological indexes of soluble sugar, catalase and the like of the medicinal rhubarb seeds germinating from the 4 th to the 8 th days, and repeating the test for three times.
As can be seen from the above table, under different component conditions, the soluble sugar of the rhubarb horsetails seeds germinating from 4 th to 8 th shows a gradual increase trend, wherein the increase is most obvious under the component E condition, which indicates that under the component condition, the storage substance starch is more beneficial to be hydrolyzed into the soluble sugar under the action of amylase and the like, and the germination and growth of the rhubarb horsetails are promoted.
Catalase is ubiquitous in plant cells and its enzymatic activity provides the cells with antioxidant defense mechanisms. The biological function of catalase is to promote the decomposition of hydrogen peroxide in cells, so that the hydrogen peroxide is not further generated to generate a great-toxicity hydroxyl radical, thereby protecting the cells from the toxicity of the hydrogen peroxide, being one of the key enzymes of a biological defense system, and having important significance on the growth and the metabolic activities of plants. As can be seen from the above table, the catalase of the rhubarb horsetails seeds germinating from 4 th to 8 th day shows a gradual increase trend under different component conditions, wherein the increase is most obvious under the component E condition, which indicates that under the component condition, the decomposition of toxic substances such as hydrogen peroxide is facilitated, the rhubarb horsetails seeds are protected from being poisoned by hydrogen peroxide, and the germination and growth of the rhubarb horsetails seeds are promoted.
And (2) a second test method:
1. nutrient soil: sterilizing at high temperature and high pressure;
2. the components are set as follows:
a: 950 parts of distilled water +50 parts of 6-benzylaminopurine.
B: 980 parts of distilled water +20 parts of auxin.
C: 950 parts of distilled water +10 parts of auxin +15 parts of cytokinin +25 parts of 6-benzylaminopurine.
D: 900 parts of distilled water, 20 parts of auxin, 30 parts of cytokinin and 50 parts of 6-benzylaminopurine.
3. Adding the above different component formulas into the flowerpot, soaking for the first time, and supplementing appropriate amount of corresponding components every 5 days at the later stage;
4. burying medicinal radix et rhizoma Rhei seed in soil, culturing at 23 deg.C in incubator under 8 hr light and 16 hr dark;
5. observing and counting the germination condition of the rhubarb seeds.
The results are shown in FIG. 3: at day 8, the medicinal rhubarb seeds of component a began to sprout roots, while the medicinal rhubarb seeds of component B, component C, component D and component E had already germinated longer roots, with the root of component E being the longest. The germinated roots of rhubarb seeds showed an overall increasing trend with time, with the trend of component E being most pronounced, with cotyledons of rhubarb seeds already protruding out of the seed coat and fully developing at day 7.
Example 1
A culture medium and a method for promoting germination of rhubarb seeds for Enshi medicine comprise the following steps:
1. sterilizing the culture dish and filter paper at high temperature and high pressure;
2. the components are set as follows: 895 parts of deionized water, 25 parts of auxin, 30 parts of cytokinin and 50 parts of 6-benzylaminopurine
3. Adding a culture solution with components on the filter paper until the filter paper is completely soaked;
4. culturing in a constant temperature incubator at 23 ℃ for 8 hours in light and 16 hours in darkness;
5. observing and counting the germination condition of the rhubarb seeds.
As a result: the germination potential of the seeds is 50 percent, the germination rate of the seeds is 98 percent, the survival rate of the seedlings is 98 percent, and the young leaves are in a dark green and healthy state. The root length on day 8 was 2.11cm, the soluble sugar content was 30.1. mu.g/g, and the catalase activity was 62.1U/g.
Example 2
A culture medium and a method for promoting germination of rhubarb seeds for Enshi medicine comprise the following steps:
1. sterilizing the culture dish and filter paper at high temperature and high pressure;
2. the components are set as follows: 895 parts of deionized water, 20 parts of auxin, 35 parts of cytokinin and 50 parts of 6-benzylaminopurine
3. Adding a culture solution with components on the filter paper until the filter paper is completely soaked;
4. culturing in a constant temperature incubator at 23 ℃ for 8 hours in light and 16 hours in darkness;
5. observing and counting the germination condition of the rhubarb seeds.
As a result: the germination potential of the seeds is 49 percent, the germination rate of the seeds is 97 percent, the survival rate of the seedlings is 98 percent, and the young leaves are in a dark green and healthy state. The root length on day 8 was 2.09cm, the soluble sugar content was 29.8. mu.g/g, and the catalase activity was 62.5U/g.
Example 3
A culture medium and a method for promoting germination of rhubarb seeds for Enshi medicine comprise the following steps:
1. sterilizing the culture dish and filter paper at high temperature and high pressure;
2. the components are set as follows: 750 parts of deionized water, 50 parts of auxin, 80 parts of cytokinin and 120 parts of 6-benzylaminopurine
3. Adding a culture solution with components on the filter paper until the filter paper is completely soaked;
4. culturing in a constant temperature incubator at 23 ℃ for 8 hours in light and 16 hours in darkness;
5. observing and counting the germination condition of the rhubarb seeds.
As a result: the germination potential of the seeds is 4 percent, the germination rate of the seeds is 13 percent, the survival rate of the seedlings is 21 percent, and the young leaves are in a light green and non-robust state. The root length on day 8 was 0.43cm, the soluble sugar content was 5.2. mu.g/g, and the catalase activity was 9.1U/g.
Test one:
20 parts of auxin, 30 parts of cytokinin, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110 and 120 parts of 6-benzylaminopurine component and 930, 920, 910, 900, 890, 880, 870, 860, 850, 840 and 830 parts of deionized water are arranged.
And (2) test II:
30 parts of cytokinin and 50 parts of 6-benzylaminopurine are arranged, the auxin components are respectively 5, 10, 15, 20, 25, 30 and 35 parts, and deionized water is 915, 910, 905, 900, 895, 890 and 885 parts.
And (3) test III:
20 parts of auxin, 50 parts of 6-benzylaminopurine, 5, 10, 20, 30, 40, 50 and 60 parts of cytokinin and 925, 920, 910, 900, 890, 880 and 870 parts of deionized water are arranged.
In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, should not be construed as limiting the invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
The above description is only for the purpose of illustrating the present invention and the appended claims are not to be construed as limiting the scope of the invention, which is intended to cover all modifications, equivalents and improvements that are within the spirit and scope of the invention as defined by the appended claims.
Claims (9)
1. The culture medium for promoting the germination of the rhubarb seeds for Enshi is characterized by comprising 15-25 parts by mass of auxin, 20-40 parts by mass of cytokinin, 40-60 parts by mass of 6-benzylaminopurine and 850-950 parts by mass of deionized or distilled water.
2. The method for promoting germination of the seeds of the rhubarb for enrichments by using the culture medium for promoting germination of the seeds of the rhubarb for enrichments of claim 1, which comprises the following steps:
step one, performing high-temperature and high-pressure sterilization treatment on a culture dish and filter paper;
step two, setting components;
adding culture solution with different components on the filter paper until the filter paper is completely soaked;
step four, culturing at constant temperature, illuminating, and culturing in darkness;
step five, counting germination data of the medicinal rhubarb seeds;
sixthly, counting root length data;
and seventhly, counting the physiological index data.
3. The method for promoting germination of rhubarb seeds for enrichments as claimed in claim 2, wherein the first step or the second step adopts:
nutrient soil: and (5) performing high-temperature and high-pressure sterilization treatment.
4. The method for promoting germination of rhubarb seeds for enrichments as claimed in claim 2, wherein the second step, component setting comprises:
a: 950 parts of deionized water +50 parts of 6-benzylaminopurine;
b: 980 parts of deionized water +20 parts of auxin;
c: 800 parts of deionized water, 40 parts of auxin, 60 parts of cytokinin and 100 parts of 6-benzylaminopurine;
d: 950 parts of deionized water +10 parts of auxin +15 parts of cytokinin +25 parts of 6-benzylaminopurine;
e: 900 parts of deionized water, 20 parts of auxin, 30 parts of cytokinin and 50 parts of 6-benzylaminopurine.
5. The method for promoting seed germination of rhubarb for enrichments as claimed in claim 2, wherein in the second step, the component arrangement further comprises:
a) the method comprises the following steps 950 parts of distilled water +50 parts of 6-benzylaminopurine;
b) the method comprises the following steps 980 parts of distilled water +20 parts of auxin;
c) the method comprises the following steps 950 parts of distilled water +10 parts of auxin +15 parts of cytokinin +25 parts of 6-benzylaminopurine;
d) the method comprises the following steps 900 parts of distilled water, 20 parts of auxin, 30 parts of cytokinin and 50 parts of 6-benzylaminopurine;
e: 900 parts of deionized water, 20 parts of auxin, 30 parts of cytokinin and 50 parts of 6-benzylaminopurine.
6. The method for promoting germination of rhubarb seeds for enrichments as claimed in claim 5, wherein after the components are set, the different component combinations are added into the flowerpot for the first soaking, and the corresponding component combinations are supplemented every 5 days in the later period.
7. The method for promoting germination of rhubarb seeds for enrichments of claim 2, wherein the fourth step specifically comprises: incubation at 23 ℃ in a constant temperature incubator, 8 hours light, 16 hours dark.
8. The method for promoting germination of rhubarb seeds for enrichments of claim 2, wherein the sixth step further comprises: the root length of the medicinal rhubarb seeds germinated from the 4 th day to the 8 th day is counted respectively, and the test is repeated for four times.
9. The method for promoting germination of rhubarb seeds for enrichments of claim 2, which further comprises the steps of: and respectively counting the physiological indexes of soluble sugar, catalase and the like of the medicinal rhubarb seeds germinating from the 4 th to the 8 th days, and repeating the test for three times.
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