CN111821913A - Device and method for preparing uniform double emulsion drops in high flux - Google Patents
Device and method for preparing uniform double emulsion drops in high flux Download PDFInfo
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- 239000000839 emulsion Substances 0.000 title claims abstract description 103
- 238000000034 method Methods 0.000 title claims abstract description 15
- 230000004907 flux Effects 0.000 title claims description 32
- 239000007788 liquid Substances 0.000 claims abstract description 196
- 238000004519 manufacturing process Methods 0.000 claims abstract description 6
- 239000000758 substrate Substances 0.000 claims description 22
- 230000005284 excitation Effects 0.000 claims description 16
- 238000002360 preparation method Methods 0.000 claims description 13
- 238000002347 injection Methods 0.000 claims description 12
- 239000007924 injection Substances 0.000 claims description 12
- 239000012530 fluid Substances 0.000 claims description 6
- 238000003860 storage Methods 0.000 claims description 6
- 230000002572 peristaltic effect Effects 0.000 claims description 4
- 239000010409 thin film Substances 0.000 claims description 4
- 230000003139 buffering effect Effects 0.000 claims description 3
- 230000006911 nucleation Effects 0.000 claims 2
- 238000010899 nucleation Methods 0.000 claims 2
- 239000002131 composite material Substances 0.000 abstract 1
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- 239000000919 ceramic Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000005538 encapsulation Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
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- 230000000737 periodic effect Effects 0.000 description 2
- 230000005501 phase interface Effects 0.000 description 2
- 230000002238 attenuated effect Effects 0.000 description 1
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Abstract
The invention discloses a device and a method for preparing uniform double emulsion drops in a high-flux manner. When the double-emulsion-type multi-phase liquid jet pump is used, the nuclear phase actuating unit inputs nuclear phase disturbance to the nuclear phase disturbance interface, the shell phase actuating unit inputs shell phase disturbance to the shell phase disturbance interface, nuclear phase liquid is discharged from the outlet end of the inner capillary structure, wrapped by shell phase liquid and discharged from the outer capillary structure, driving phase liquid is wrapped outside the shell phase liquid and discharged from the discharge hole to form a composite jet flow, and the shell phase liquid wraps the nuclear phase liquid and is broken down due to instability at the downstream to form double-emulsion droplets. According to the invention, the nuclear phase actuating unit and the shell phase actuating unit respectively input disturbance to the nuclear phase liquid and the shell phase liquid, so that the size of the double-emulsion droplets is controlled, the high-flux production of the double-emulsion droplets is realized, and the monodispersity of the sizes of the double-emulsion droplets is ensured.
Description
Technical Field
The invention relates to the technical field of double-emulsion drop preparation, in particular to a device and a method for preparing uniform double-emulsion drops in a high-flux manner.
Background
The double emulsion droplets are complex droplets with dispersed phase droplets wrapped with smaller droplets, and the outer droplets form a shielding layer around the inner droplets to effectively isolate the inner droplets from the continuous phase. Double emulsion droplets, which are different from single emulsion droplets, have more flexible structural features, due to their unique core-shell structure and material selectivity, have attracted increasing research interest to scientists. The encapsulation with double emulsion drops as templates can be effectively applied to the fields of encapsulation, transportation or controllable release, such as cells, biochemistry, catalysts, self-healing materials, carbonless copy paper, electronic ink, carbon dioxide adsorption and the like.
At present, when double emulsion droplets are produced at high flux, the droplet mode is generally required to be converted into a jet flow mode, so that the size distribution of the droplets is widened, and the breaking length of the jet flow is random to some extent.
Therefore, how to realize high-throughput production of double emulsion droplets and ensure uniform size of double emulsion droplets is a technical problem to be solved urgently by those skilled in the art.
Disclosure of Invention
In view of the above, the first object of the present invention is to provide an apparatus for preparing uniform double emulsion droplets at high throughput, which can achieve high-throughput production of double emulsion droplets and ensure uniform size of the double emulsion droplets.
A second object of the present invention is to provide a method for high throughput preparation of homogeneous double emulsion droplets.
In order to achieve the first object, the present invention provides the following solutions:
a device for preparing uniform double emulsion drops in high flux comprises a base, a cavity, an inner capillary structure, an outer capillary structure, a nuclear phase actuating unit and a shell phase actuating unit;
the base is provided with a nuclear phase liquid channel, the inlet end of the nuclear phase liquid channel is used for inputting nuclear phase liquid, the inlet end of the inner capillary structure is in conduction connection with the outlet end of the nuclear phase liquid channel, the base is provided with a nuclear phase disturbance interface which is in conduction with the nuclear phase liquid channel, the nuclear phase disturbance interface is packaged, and the nuclear phase actuating unit inputs nuclear phase disturbance through the packaged nuclear phase disturbance interface;
the base is further provided with a shell phase liquid channel, the inlet end of the shell phase liquid channel is used for inputting shell phase liquid, the inlet end of the outer capillary structure is communicated with the outlet end of the shell phase liquid channel, the outer capillary structure is arranged around the inner capillary structure, the inner capillary structure and the outer capillary structure are concentrically arranged, the base is provided with a shell phase disturbance interface communicated with the shell phase liquid channel, the shell phase disturbance interface is packaged, and the shell phase actuating unit inputs shell phase disturbance through the packaged shell phase disturbance interface;
the cavity is hermetically arranged at the bottom end of the base, and the outer capillary structure is arranged in the cavity;
the cavity is provided with a driving phase inlet, the driving phase inlet is used for inputting driving phase liquid into the cavity, and the shell phase liquid is immiscible with the driving phase liquid and the nuclear phase liquid respectively;
the bottom end of the cavity is provided with a discharge hole, and the discharge hole is concentric with the outlet end of the outer capillary structure.
In a particular embodiment, the outlet end of the inner capillary structure is located above or below the outlet end of the outer capillary structure;
and/or
The diameter D of the double emulsion droplet to be prepared is (6Q/. pi.f)1/3，
Q＝Qo+Qi，
Wherein Q is the sum of the flow rates of the shell phase liquid and the core phase liquid,
Qothe flow rate of the shell phase is the flow rate of the shell phase,
Qithe flow rate of the shell phase is the flow rate of the shell phase,
f is the excitation frequency;
and/or
The diameter d of the inner core of the double emulsion droplet to be prepared is (6Q)i/πf)1/3，
Wherein Q isiThe flow rate of the shell phase is the flow rate of the shell phase,
f is the excitation frequency.
In another embodiment, the device for preparing uniform double emulsion droplets in high flux further comprises a nuclear phase substrate and a nuclear phase support column,
one end of the nuclear phase supporting column is installed on the base, the nuclear phase substrate is installed on the other end of the nuclear phase supporting column, the nuclear phase actuating unit is installed on the nuclear phase substrate, and the actuating end of the nuclear phase actuating unit inputs nuclear phase disturbance to the nuclear phase disturbance interface;
and/or
The device for preparing the uniform double emulsion drops in high flux also comprises a shell phase substrate and a shell phase supporting column,
one end of the shell phase supporting column is installed on the base, the shell phase substrate is installed on the other end of the shell phase supporting column, the shell phase actuating unit is installed on the shell phase substrate, and the actuating end of the shell phase actuating unit inputs shell phase disturbance to the shell phase disturbance interface.
In another embodiment, the nuclear phase actuation unit comprises a nuclear phase signal generator, a nuclear phase power amplifier and a nuclear phase actuator,
the nuclear phase signal generator is in signal connection with the nuclear phase power amplifier, the nuclear phase power amplifier is in signal connection with the nuclear phase actuator,
a nuclear phase disturbance signal sent by the nuclear phase signal generator is amplified by the nuclear phase power amplifier and then is output to the nuclear phase disturbance interface through the nuclear phase actuator;
and/or
The shell phase actuating unit comprises a shell phase signal generator, a shell phase power amplifier and a shell phase actuator,
the shell-phase signal generator is in signal connection with the shell-phase power amplifier, the shell-phase power amplifier is in signal connection with the shell-phase actuator,
and after being amplified by the shell phase power amplifier, the shell phase disturbance signal sent by the shell phase signal generator is output to the shell phase disturbance interface through the shell phase actuator.
In another embodiment, the core phase perturbation interface and the shell phase perturbation interface are both encapsulated by thin films.
In another specific embodiment, the device for preparing uniform double emulsion droplets at high flux further comprises a first injection pump, wherein the first injection pump is in communication connection with the inlet end of the nuclear phase liquid channel and is used for injecting nuclear phase liquid into the nuclear phase liquid channel;
the device for preparing the uniform double emulsion drops in high flux further comprises a second injection pump, wherein the second injection pump is communicated and connected with the inlet end of the shell phase liquid channel and is used for injecting shell phase liquid into the shell phase liquid channel;
the device for preparing the uniform double emulsion drops in high flux further comprises a third injection pump, wherein the third injection pump is in conduction connection with the driving phase inlet and is used for injecting the driving phase liquid into the cavity, or
The device for preparing the uniform double emulsion drops at high flux also comprises a power pump and a buffer;
the power pump is in conductive connection with the buffer, and the buffer is in conductive connection with the driving phase inlet;
the power pump is used for conveying the driving phase liquid into the buffer, and the buffer is used for buffering the driving phase liquid and conveying the driving phase liquid into the cavity.
In another specific embodiment, the high throughput apparatus for preparing uniform double emulsion droplets further comprises a reservoir;
the liquid storage tank is used for storing the driving phase liquid;
the power pump is a peristaltic pump, and the buffer is a buffer bottle.
In another specific embodiment, the device for preparing uniform double emulsion drops by high flux further comprises an image acquisition module,
the image acquisition module is used for acquiring image signals of double emulsion drops dripped from the discharge hole;
and/or
The device for preparing uniform double emulsion drops in high flux also comprises a drop collection module,
the liquid drop collecting module is arranged at the bottom end of the base and used for collecting double emulsion drops dropping from the discharge hole.
The various embodiments according to the invention can be combined as desired, and the embodiments obtained after these combinations are also within the scope of the invention and are part of the specific embodiments of the invention.
Without being limited to any theory, it can be seen from the above disclosure that, in the high-throughput device for preparing uniform double-emulsion droplets disclosed by the invention, when in use, the driving phase liquid is firstly input into the cavity, and a stable driving phase flow field is formed, then, the nuclear phase liquid and the shell phase liquid are respectively introduced into the nuclear phase liquid channel and the shell phase liquid channel, the nuclear phase disturbance is input into the nuclear phase disturbance interface by the nuclear phase actuating unit, so that the control of nuclear phase liquid jet flow crushing is realized, the shell phase disturbance is input into the shell phase disturbance interface by the shell phase actuating unit, so that the control of shell phase liquid jet flow crushing is realized, the nuclear phase liquid is discharged from the outlet end of the inner capillary structure, is wrapped by the shell phase liquid, is discharged from the outlet end of the outer capillary structure, and is wrapped by the driving phase liquid at the outer end of the shell phase liquid and is discharged from the discharge hole, so that double. According to the invention, the nuclear phase actuating unit and the shell phase actuating unit respectively input and disturb to the nuclear phase liquid and the shell phase liquid, so that the size of the double-emulsion droplets is controlled, the high-flux production of the double-emulsion droplets is realized, and the uniform size of the double-emulsion droplets is ensured.
In order to achieve the second object, the invention provides the following technical solutions:
a method for high throughput preparation of uniform double emulsion droplets comprising the steps of:
continuously inputting driving phase liquid to form a stable flow field;
respectively and continuously inputting core phase liquid and shell phase liquid, and respectively inputting a core phase disturbance signal and a shell phase disturbance signal into the core phase liquid and the shell phase liquid, wherein a capillary outlet of the shell phase liquid surrounds a capillary outlet of the core phase liquid, the shell phase liquid covers the core phase liquid, and the shell phase liquid and the core phase liquid are not mutually soluble;
the outlet of the driving phase liquid surrounds the capillary outlet of the shell phase liquid, the driving phase liquid and the shell phase liquid are not mutually soluble, and the driving phase liquid is coated outside the shell phase liquid to form double emulsion drops.
In a particular embodiment, the diameter D ═ (6Q/pi f) of the double emulsion droplets1/3，
Q＝Qo+Qi，
Wherein Q is the sum of the flow rates of the shell phase liquid and the core phase liquid,
Qothe flow rate of the shell phase is the flow rate of the shell phase,
Qithe flow rate of the shell phase is the flow rate of the shell phase,
f is the excitation frequency;
and/or
The diameter d of the inner core of the double emulsion drop is (6Q)i/πf)1/3，
Wherein Q isiThe flow rate of the shell phase is the flow rate of the shell phase,
f is the excitation frequency.
According to the method for preparing the uniform double-emulsion droplets in a high-flux manner, the nuclear phase disturbance signal and the shell phase disturbance signal are input into the nuclear phase liquid and the shell phase liquid, so that the size of the double-emulsion droplets is controlled, the double-emulsion droplets are produced in a high-flux manner, and the uniform size of the double-emulsion droplets is ensured.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a device for preparing uniform double emulsion droplets in high flux according to the present invention;
FIG. 2 is a schematic view of the enlarged partial structure of FIG. 1 when the inner capillary tube is lower than the outer capillary tube;
FIG. 3 is an enlarged partial schematic view of FIG. 1 with the inner capillary tube higher than the outer capillary tube;
FIG. 4 is a micrograph of a typical droplet provided by the present invention;
FIG. 5 is a graph of particle size versus frequency provided by the present invention;
FIG. 6 is a schematic diagram of the apparatus for high throughput preparation of uniform single emulsion droplets provided by the present invention;
fig. 7 is a schematic structural diagram of a droplet collection module provided by the present invention.
Wherein, in fig. 1-7:
the device comprises a base 1, a cavity 2, an inner capillary structure 3, an outer capillary structure 4, a nuclear phase actuating unit 5, a shell phase actuating unit 6, a nuclear phase liquid channel 101, a nuclear phase perturbation interface 102, a shell phase liquid channel 103, a shell phase perturbation interface 104, a driving phase inlet 201, a discharge hole 202, a nuclear phase substrate 7, a nuclear phase support column 8, a shell phase substrate 9, a shell phase support column 10, a first seal ring 11, a second seal ring 12, a first injection pump 13, a second injection pump 14, a third injection pump 15, a nuclear phase signal generator 501, a nuclear phase power amplifier 502, a shell phase signal generator 601 and a shell phase power amplifier 602.
Detailed Description
In order to make the technical solutions of the present invention better understood by those skilled in the art, the present invention will be further described in detail with reference to the accompanying fig. 1 to 7 and the detailed description thereof.
As shown in figures 1-3, the invention discloses a device for preparing uniform double emulsion drops in high flux, wherein the device for preparing uniform double emulsion drops in high flux comprises a base 1, a cavity 2, an inner capillary structure 3, an outer capillary structure 4, a nuclear phase actuating unit 5 and a shell phase actuating unit 6.
The nuclear phase liquid channel 101 is formed in the base 1, the inlet end of the nuclear phase liquid channel 101 is used for inputting nuclear phase liquid, the inlet end of the inner capillary structure 3 is in conduction connection with the outlet end of the nuclear phase liquid channel 101, specifically, the inner capillary structure 3 and the base 1 can be integrally formed and connected, and the connection strength and the sealing performance of the inner capillary structure 3 and the base 1 are improved. It should be noted that the inner capillary structure 3 and the base 1 may be detachably connected.
The base 1 is provided with a nuclear phase disturbance interface 102 communicated with the nuclear phase liquid channel 101, the nuclear phase disturbance interface 102 is packaged, and the nuclear phase actuating unit 5 inputs nuclear phase disturbance through the packaged nuclear phase disturbance interface 102. Specifically, the nuclear phase perturbation interface 102 is encapsulated by a thin film so as to input nuclear phase perturbation to the nuclear phase perturbation interface 102.
Shell phase liquid channel 103 has still been seted up on base 1, shell phase liquid channel 103's entry end is used for the input of shell phase liquid, outer capillary structure 4's entry end and shell phase liquid channel 103's exit end turn-on connection, outer capillary structure 4 encircles interior capillary structure 3 and sets up, specifically, the top of outer capillary structure 4 and the bottom sealing connection of base 1, outer capillary structure 4 cover is established outside interior capillary structure 3, and interior capillary structure 3 sets up with outer capillary structure 4 is concentric.
The draw-in groove is seted up on the top of outer capillary 4, and first sealing washer 11 is arranged in the draw-in groove, and the bottom butt of top and base 1 to sealed outer capillary 4 and base 1.
The base 1 is provided with a shell phase disturbance interface 104 communicated with the shell phase liquid channel 103, the shell phase disturbance interface 104 is packaged, and the shell phase actuating unit 6 inputs shell phase disturbance through the packaged shell phase disturbance interface 104. Specifically, the shell phase interface is encapsulated by a thin film so as to input shell phase disturbances to the shell phase interface.
The cavity 2 is hermetically arranged at the bottom end of the base 1, and the outer capillary structure 4 is arranged in the cavity 2. Specifically, the holding tank has been seted up on the top of cavity 2, and in the holding tank was arranged in to second sealing washer 12, and the bottom butt of the top of second sealing washer 12 and base 1 to the realization is to cavity 2 and base 1's sealing.
The cavity 2 is provided with a driving phase inlet 201, the driving phase inlet 201 is used for inputting driving phase liquid into the cavity 2, and the shell phase liquid is insoluble with the driving phase liquid and the core phase liquid respectively.
The bottom end of the cavity 2 is provided with a discharge hole 202 which is concentric with the outlet end of the outer capillary structure 4.
When the device for preparing uniform double emulsion drops with high flux is used, driving phase liquid is firstly input into a cavity 2, a stable driving phase flow field is formed, then, nuclear phase liquid and shell phase liquid are respectively introduced into a nuclear phase liquid channel 101 and a shell phase liquid channel 103, nuclear phase disturbance is input into a nuclear phase disturbance interface 102 by a nuclear phase actuating unit 5, the control of nuclear phase liquid jet flow crushing is realized, shell phase disturbance is input into a shell phase disturbance interface 104 by a shell phase actuating unit 6, the control of shell phase liquid jet flow crushing is realized, the nuclear phase liquid is discharged from the outlet end of an inner capillary structure 3, is wrapped by the shell phase liquid and is discharged from the outlet end of an outer capillary structure 4, and the driving phase liquid is wrapped at the outer end of the shell phase liquid and is discharged from a discharge hole 202 to form double emulsion drops. According to the invention, the nuclear phase actuating unit 5 and the shell phase actuating unit 6 are respectively used for inputting disturbance to the nuclear phase liquid and the shell phase liquid, so that the size of the double-emulsion droplets is controlled, the high-flux production of the double-emulsion droplets is realized, and the uniform size of the double-emulsion droplets is ensured.
In some of these embodiments, the present invention discloses that the outlet end of the inner capillary structure 3 is located above or below the outlet end of the outer capillary structure 4. As shown in fig. 2 and 3. Preferably, the outlet end of the outer capillary is slightly lower than the outlet end of the inner capillary.
In some of these examples, the diameter D ═ (6Q/pi f) of the double emulsion droplets to be prepared1/3，Q＝Qo+QiWherein Q is the sum of the flow rates of the shell phase liquid and the core phase liquid, QoIs shell phase flow, QiShell phase flow, and f is the excitation frequency.
It should be noted that the disturbance may be applied to any of the shell phase and the shell phase, and may of course be applied simultaneously. The initial perturbation may be an axial perturbation, i.e. a periodic modulation of the flow velocity of the jet by externally coupled energy (mechanical, electric, magnetic, etc.).
The excitation frequency of the initial disturbance is set to be optimal near the natural breaking frequency of the jet flow, the natural breaking frequency can be changed by changing the structural parameters and the fluid properties, so that the interval of the disturbance applying frequency is adjusted, and when the external disturbance can lead the jet flow to break, the volume of the generated liquid drops is completely controlled by the shell phase flow QoNuclear phase flow rate QiTogether with the excitation frequency f, a typical drop microscope is shown in fig. 4.
The inner core of the double emulsion drop to be prepared can be independently controlled, and the diameter d of the inner core is (6Q)i/πf)1/3Wherein Q isiShell phase flow, and f is the excitation frequency.
In preparing double emulsion droplets, double emulsion droplets can be prepared at high throughput. Typically, the single flux for the shell phase liquid channel 103 and the core phase liquid channel 101 can be selected between 1ml/h and 1000ml/h, as shown in FIG. 5.
In some embodiments, the device for preparing uniform double emulsion droplets at high flux further comprises a nuclear phase substrate 7 and a nuclear phase support column 8, one end of the nuclear phase support column 8 is installed on the base 1, the nuclear phase substrate 7 is installed on the other end of the nuclear phase support column 8, the nuclear phase actuation unit 5 is installed on the nuclear phase substrate 7, and the actuation end of the nuclear phase actuation unit 5 inputs nuclear phase disturbance to the nuclear phase disturbance interface 102. Specifically, the nuclear phase support column 8 is welded to the base 1, and may be detachably connected to the base 1 by a screw or the like. The nuclear phase substrate 7 can be directly welded on the nuclear phase support column 8, and can also be detachably connected on the nuclear phase support column 8 through screws and the like.
Further, the invention discloses a device for preparing uniform double emulsion droplets in a high flux manner, which further comprises a shell phase substrate 9 and a shell phase support column 10, wherein one end of the shell phase support column 10 is installed on the base 1, the shell phase substrate 9 is installed on the other end of the shell phase support column 10, the shell phase actuating unit 6 is installed on the shell phase substrate 9, and the actuating end of the shell phase actuating unit 6 inputs shell phase disturbance to the shell phase disturbance interface 104. Specifically, the shell phase support column 10 is welded to the base 1, and may be detachably attached to the base 1 by a screw or the like. The shell phase substrate 9 may be directly welded to the shell phase support posts 10, or may be detachably attached to the shell phase support posts 10 by screws or the like.
In some embodiments, the nuclear phase actuation unit 5 includes a nuclear phase signal generator 501, a nuclear phase power amplifier 502, and a nuclear phase actuator, where the nuclear phase signal generator 501 is in signal connection with the nuclear phase power amplifier 502, the nuclear phase power amplifier 502 is in signal connection with the nuclear phase actuator, and a nuclear phase disturbance signal sent by the nuclear phase signal generator 501 is amplified by the nuclear phase power amplifier 502 and then output to the nuclear phase disturbance interface 102 through the nuclear phase actuator.
The disturbance signal output by the nuclear phase signal generator 501 may be a sine signal, a sawtooth signal, a rectangular wave signal, or the like, preferably a sine modulation; it is also possible to combine multiple modulations, preferably with the same period, although other combinations of signals are possible.
The nuclear phase actuator may take many forms, for example, a piezoelectric ceramic, an electromagnetic oscillator, or the like, which may provide a unit of vibration.
Further, the invention discloses that the shell phase actuating unit 6 comprises a shell phase signal generator 601, a shell phase power amplifier 602 and a shell phase actuator, wherein the shell phase signal generator 601 is in signal connection with the shell phase power amplifier 602, the shell phase power amplifier 602 is in signal connection with the shell phase actuator, and a shell phase disturbance signal sent by the shell phase signal generator 601 is amplified by the shell phase power amplifier 602 and then is output to the shell phase disturbance interface 104 through the shell phase actuator.
The disturbing signal output by the shell phase signal generator 601 may be a sinusoidal signal, a sawtooth wave signal, a rectangular wave signal, etc., preferably a sinusoidal modulation; it is also possible to combine multiple modulations, preferably with the same period, although other combinations of signals are possible.
The shell phase actuator may take various forms, for example, a piezoelectric ceramic, an electromagnetic oscillator, or the like, which may provide a unit that vibrates.
In other embodiments, the present invention discloses that the apparatus for preparing uniform double emulsion droplets at high flux further comprises a power pump and a buffer, the power pump is in conductive connection with the buffer, the buffer is in conductive connection with the driving phase inlet 201, the power pump is used for delivering the driving phase liquid into the buffer, and the buffer is used for buffering the driving phase liquid and delivering the driving phase liquid into the cavity 2. The pulse flow of the power pump is attenuated after passing through the buffer, and the single emulsion droplet particle size influence parameter is not influenced by the flow of the driving phase liquid within a certain range. Specifically, the invention discloses that the power pump is a peristaltic pump, and the buffer is a buffer bottle. The power pump is not limited to the peristaltic pump described above, and may be another type of pump. The buffer bottle is not limited to the bottle structure, but may be other structures.
Note that, each phase liquid may be directly injected by a syringe pump, and a syringe pump for injecting a nuclear phase liquid may be used as the first syringe pump 13, a syringe pump for injecting a shell phase liquid may be used as the second syringe pump 14, and a syringe pump for injecting a driving phase liquid may be used as the third syringe pump 15, as shown in fig. 6.
Further, the device for preparing uniform double emulsion drops in high flux also comprises a liquid storage tank, and the liquid storage tank is used for driving the storage of phase liquid. Under the condition of needing a large amount of driving phase liquid, only the driving phase liquid is needed to be added into the liquid storage tank, and the method has important practical value when single emulsion drops are continuously prepared in a large scale.
In some embodiments, the apparatus for high-throughput preparation of uniform double emulsion droplets further comprises an image acquisition module for acquiring image signals of double emulsion droplets dripped from the discharge hole 202. The image collector is positioned below the cavity 2 and used for collecting image information and transmitting the collected information to the controller. The image collector is a camera or a video camera and the like, and the controller is a computer and the like.
In order to collect the signal of the single emulsion drop, the invention discloses a device for preparing uniform double emulsion drops in high flux, which further comprises a strobe light, wherein the strobe light is arranged below the cavity 2 and is positioned on one side of the single emulsion drop.
Further, the present invention discloses that the apparatus for preparing uniform double emulsion droplets at high throughput further comprises a droplet collecting module, as shown in fig. 7, which is disposed at the bottom end of the base 1 and is used for collecting double emulsion droplets from the discharge holes 202. Specifically, the droplet collection module comprises a container, wherein a collection liquid is contained in the container, and the collection liquid and the driving phase liquid are immiscible.
The initial disturbance (including frequency and amplitude) of the jet flow is actively controlled by the shell phase actuating unit 6 and the nuclear phase actuating unit 5, the fluid flowing out from the upstream is dominated by the external disturbance, and the disturbance can control the breaking frequency of the jet flow. The invention can accurately control the jet flow crushing process, can control the particle size of liquid drops and the length of the jet flow, and can be widely used by adjusting the frequency interval of disturbance application by adjusting the natural crushing frequency. The invention can also be applied to fluids of different properties (viscosity, interfacial tension), in particular to the situation of different fluids in various practical applications.
According to the device for preparing the uniform double emulsion drops in the high flux, the shell phase actuating unit 6 and the nuclear phase actuating unit 5 are adopted to control the initial disturbance, so that the precise control of the jet flow crushing process can be realized, particularly the crushing frequency of the jet flow is controlled, the device is widely applied to various droplet micro-fluidic technologies, the defect that the flux cannot be considered by depending on a drop mode in the existing micro-fluidic technology for preparing the uniform drops can be effectively overcome, and the device is particularly suitable for being used in a scene of micro-drops which need to be prepared in a large amount by using a jet flow mode.
Another aspect of the present invention provides a method for high throughput preparation of uniform double emulsion droplets, comprising the steps of:
step S1: continuously inputting driving phase liquid to form a stable flow field.
Step S2: the method comprises the steps of continuously inputting core phase liquid and shell phase liquid respectively, and inputting core phase disturbance signals and shell phase disturbance signals into the core phase liquid and the shell phase liquid respectively, wherein a capillary outlet of the shell phase liquid surrounds a capillary outlet of the core phase liquid, the shell phase liquid covers the core phase liquid, and the shell phase liquid and the core phase liquid are not mutually soluble.
Step S3: the outlet of the driving phase liquid surrounds the capillary outlet of the shell phase liquid, the driving phase liquid and the shell phase liquid are not mutually soluble, and the driving phase liquid is coated outside the shell phase liquid to form double emulsion drops.
The excitation frequency of the initial disturbance is set to be optimal near the natural breaking frequency of the jet flow, the natural breaking frequency can be changed by changing structural parameters, fluid properties, flow parameters and the like, so that the interval of the disturbance applying frequency is adjusted, and when the external disturbance can lead the jet flow to break, the volume of the generated liquid drops is completely controlled by the shell phase flow QoNuclear phase flow rate QiTogether with the excitation frequency f.
Diameter D ═ (6Q/. pi.f) of the double emulsion droplets to be prepared1/3，Q＝Qo+QiWherein Q is the sum of the flow rates of the shell phase liquid and the core phase liquid, QoIs shell phase flow, QiShell phase flow, and f is the excitation frequency.
It should be noted that the disturbance may be applied to any of the shell phase and the core phase, and may of course be applied simultaneously. The initial perturbation may be an axial perturbation, i.e. a periodic modulation of the flow velocity of the jet by externally coupled energy (mechanical, electric, magnetic, etc.).
The inner core of the double emulsion drop to be prepared can be independently controlled, and the diameter d of the inner core is (6Q)i/πf)1/3Wherein Q isiShell phase flow, and f is the excitation frequency.
The invention discloses a method for preparing uniform double-emulsion droplets in high flux, which realizes the control of the jet flow crushing of dispersed phase liquid by inputting disturbance signals to the dispersed phase liquid, and forms single-emulsion droplets by wrapping of driving phase liquid. The preparation of different single emulsion drops is realized by controlling the frequency of the disturbance signal, and the adjustment and control of the volume of the single emulsion drops are realized. In addition, the defect that flux cannot be considered by depending on a drop mode in order to prepare uniform liquid drops in the existing microfluidics can be effectively overcome by controlling the breaking frequency of jet flow.
The terms "first", "second", and the like in the present invention are used for descriptive distinction and have no other special meaning.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and inventive features disclosed herein.
Claims (10)
1. A device for preparing uniform double emulsion drops in high flux is characterized by comprising a base, a cavity, an inner capillary structure, an outer capillary structure, a nuclear phase actuating unit and a shell phase actuating unit;
the base is provided with a nuclear phase liquid channel, the inlet end of the nuclear phase liquid channel is used for inputting nuclear phase liquid, the inlet end of the inner capillary structure is in conduction connection with the outlet end of the nuclear phase liquid channel, the base is provided with a nuclear phase disturbance interface which is in conduction with the nuclear phase liquid channel, the nuclear phase disturbance interface is packaged, and the nuclear phase actuating unit inputs nuclear phase disturbance through the packaged nuclear phase disturbance interface;
the base is further provided with a shell phase liquid channel, the inlet end of the shell phase liquid channel is used for inputting shell phase liquid, the inlet end of the outer capillary structure is communicated with the outlet end of the shell phase liquid channel, the outer capillary structure is arranged around the inner capillary structure, the inner capillary structure and the outer capillary structure are concentrically arranged, the base is provided with a shell phase disturbance interface communicated with the shell phase liquid channel, the shell phase disturbance interface is packaged, and the shell phase actuating unit inputs shell phase disturbance through the packaged shell phase disturbance interface;
the cavity is hermetically arranged at the bottom end of the base, and the outer capillary structure is arranged in the cavity;
the cavity is provided with a driving phase inlet, the driving phase inlet is used for inputting driving phase liquid into the cavity, and the shell phase liquid is immiscible with the driving phase liquid and the nuclear phase liquid respectively;
the bottom end of the cavity is provided with a discharge hole, and the discharge hole is concentric with the outlet end of the outer capillary structure.
2. High throughput uniform double emulsion droplet preparation apparatus according to claim 1, wherein the outlet end of said inner capillary structure is located above or below the outlet end of said outer capillary structure;
and/or
The diameter D of the double emulsion droplet to be prepared is (6Q/. pi.f)1/3，
Q＝Qo+Qi，
Wherein Q is the sum of the flow rates of the shell phase liquid and the core phase liquid,
Qothe flow rate of the shell phase is the flow rate of the shell phase,
Qithe flow rate of the shell phase is the flow rate of the shell phase,
f is the excitation frequency;
and/or
The inner core of the double emulsion drops to be preparedDiameter d ═ 6Qi/πf)1/3，
Wherein Q isiThe flow rate of the shell phase is the flow rate of the shell phase,
f is the excitation frequency.
3. The device for high throughput preparation of uniform double emulsion droplets of claim 1, further comprising a nucleation phase substrate and a nucleation phase support column,
one end of the nuclear phase supporting column is installed on the base, the nuclear phase substrate is installed on the other end of the nuclear phase supporting column, the nuclear phase actuating unit is installed on the nuclear phase substrate, and the actuating end of the nuclear phase actuating unit inputs nuclear phase disturbance to the nuclear phase disturbance interface;
and/or
The device for preparing the uniform double emulsion drops in high flux also comprises a shell phase substrate and a shell phase supporting column,
one end of the shell phase supporting column is installed on the base, the shell phase substrate is installed on the other end of the shell phase supporting column, the shell phase actuating unit is installed on the shell phase substrate, and the actuating end of the shell phase actuating unit inputs shell phase disturbance to the shell phase disturbance interface.
4. High throughput uniform double emulsion droplets preparation device according to claim 3 wherein said nuclear phase actuation unit comprises a nuclear phase signal generator, a nuclear phase power amplifier and a nuclear phase actuator,
the nuclear phase signal generator is in signal connection with the nuclear phase power amplifier, the nuclear phase power amplifier is in signal connection with the nuclear phase actuator,
a nuclear phase disturbance signal sent by the nuclear phase signal generator is amplified by the nuclear phase power amplifier and then is output to the nuclear phase disturbance interface through the nuclear phase actuator;
and/or
The shell phase actuating unit comprises a shell phase signal generator, a shell phase power amplifier and a shell phase actuator,
the shell-phase signal generator is in signal connection with the shell-phase power amplifier, the shell-phase power amplifier is in signal connection with the shell-phase actuator,
and after being amplified by the shell phase power amplifier, the shell phase disturbance signal sent by the shell phase signal generator is output to the shell phase disturbance interface through the shell phase actuator.
5. The device for high-throughput preparation of uniform double emulsion droplets according to claim 4, wherein the nuclear phase perturbation interface and the shell phase perturbation interface are both encapsulated by thin films.
6. The device for high-throughput preparation of uniform double emulsion droplets according to claim 1, further comprising a first syringe pump, wherein the first syringe pump is in fluid connection with the inlet end of the nuclear phase liquid channel and is used for injecting nuclear phase liquid into the nuclear phase liquid channel;
the device for preparing the uniform double emulsion drops in high flux further comprises a second injection pump, wherein the second injection pump is communicated and connected with the inlet end of the shell phase liquid channel and is used for injecting shell phase liquid into the shell phase liquid channel;
the device for preparing the uniform double emulsion drops in high flux also comprises a third injection pump which is communicated and connected with the driving phase inlet and is used for injecting the driving phase liquid into the cavity,
or
The device for preparing uniform double emulsion drops at high flux also comprises a power pump and a buffer,
the power pump is in conductive connection with the buffer, the buffer is in conductive connection with the driving phase inlet,
the power pump is used for conveying the driving phase liquid into the buffer, and the buffer is used for buffering the driving phase liquid and conveying the driving phase liquid into the cavity.
7. The high throughput uniform double emulsion droplet generator of claim 6, further comprising a reservoir;
the liquid storage tank is used for storing the driving phase liquid;
the power pump is a peristaltic pump, and the buffer is a buffer bottle.
8. High throughput device for preparing uniform double emulsion droplets according to any one of claims 1 to 7, further comprising an image acquisition module,
the image acquisition module is used for acquiring image signals of double emulsion drops dripped from the discharge hole;
and/or
The device for preparing uniform double emulsion drops in high flux also comprises a drop collection module,
the liquid drop collecting module is arranged at the bottom end of the base and used for collecting double emulsion drops dropping from the discharge hole.
9. A method for high throughput production of uniform double emulsion droplets comprising the steps of:
continuously inputting driving phase liquid to form a stable flow field;
respectively and continuously inputting core phase liquid and shell phase liquid, and respectively inputting a core phase disturbance signal and a shell phase disturbance signal into the core phase liquid and the shell phase liquid, wherein a capillary outlet of the shell phase liquid surrounds a capillary outlet of the core phase liquid, the shell phase liquid covers the core phase liquid, and the shell phase liquid and the core phase liquid are not mutually soluble;
the driving phase liquid surrounds the capillary outlet of the shell phase liquid, the driving phase liquid and the shell phase liquid are not mutually soluble, and the driving phase liquid is coated outside the shell phase liquid to form double emulsion drops.
10. High throughput method for the preparation of homogeneous double emulsion droplets according to claim 9, wherein the diameter of the double emulsion droplets D ═ (6Q/π f)1/3，
Q＝Qo+Qi，
Wherein Q is the sum of the flow rates of the shell phase liquid and the core phase liquid,
Qothe flow rate of the shell phase is the flow rate of the shell phase,
Qithe flow rate of the shell phase is the flow rate of the shell phase,
f is the excitation frequency;
and/or
The diameter d of the inner core of the double emulsion drop is (6Q)i/πf)1/3，
Wherein Q isiThe flow rate of the shell phase is the flow rate of the shell phase,
f is the excitation frequency.
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