CN111821326A

CN111821326A - Application of photorhabdus alternatus flower

Info

Publication number: CN111821326A
Application number: CN201910323502.0A
Authority: CN
Inventors: 康梦实; 戚微岩; 于瑞河
Original assignee: Anhui Dexin Huahai Biotechnology Co ltd
Current assignee: Anhui Dexin Huahai Biotechnology Co ltd
Priority date: 2019-04-22
Filing date: 2019-04-22
Publication date: 2020-10-27

Abstract

The invention discloses an application of photophyllanthus urinaria, belonging to the technical field of medicines. The invention mainly opens the fresh branches and leaves of the luminous leafflower, has low cost, obvious social value and market value and good development and application prospect. The flower and/or bract and/or branch and/or leaf of the glabrous leafflower can improve the activity of acetaldehyde dehydrogenase, inhibit the abnormal rise of glutamic-pyruvic transaminase and glutamic-oxalacetic transaminase caused by alcohol, and has the effects of relieving alcoholism and protecting liver; meanwhile, the invention can reduce the blood sugar and blood fat of mice with type II diabetes, improve the oral glucose tolerance, obviously reduce the insulin resistance and the like, can be used for preparing health-care food with the blood sugar and blood fat reducing and insulin resistance relieving functions, and has the health-care functions of reducing blood sugar and blood fat for the crowds with type II diabetes, such as hyperglycemia, hyperlipidemia, insulin resistance patients and the like. The health food has low adverse side effect and stable quality.

Description

Application of photorhabdus alternatus flower

Technical Field

The invention belongs to the field of medical health-care products, and particularly relates to a health-care food which can improve the activity of acetaldehyde dehydrogenase, inhibit the abnormal rise of glutamic-pyruvic transaminase and glutamic-oxalacetic transaminase caused by alcohol, has the effects of reducing blood sugar, reducing blood fat, improving glucose tolerance and reducing insulin resistance, and can be used for preparing alcohol-relieving liver-protecting type, blood sugar-reducing type, blood fat-reducing type and insulin resistance-reducing type health-care foods.

Background

China is a large country for wine production and consumption, the consumption of wine beverages reaches more than ten thousand tons every year, about hundred million people suffering from alcohol addiction or alcohol dependence, and the acute and chronic alcoholism rate is increasingly increased.

Alcoholism is mainly caused by ethanol, the main component of wine, and metabolic disturbance thereof. The liver is the major site of ethanol metabolism. After the ethanol enters the liver, acetaldehyde is firstly generated by the metabolism of ethanol dehydrogenase, then the acetaldehyde is metabolized into acetic acid by the acetaldehyde dehydrogenase, and finally the acetic acid is decomposed into carbon dioxide and water. Acetaldehyde dehydrogenase plays an important role in the metabolism of acetaldehyde, but only ALDH having an oxidizing effect in human body₁And ALDH₂Two isoenzymes, wherein ALDH₂Is an isozyme with the strongest physiological activity in ALDH. The data show that 50% of Chinese are deficient in ALDH₂After drinking, the concentration of acetaldehyde is easily increased and accumulated, so that the antigens of the liver cell membrane are changed, the metabolism of liver cells is disturbed, and free radicals, lipid peroxides and the like are generated. Failure to produce normal acetaldehyde dehydrogenase in vivo is one of the main causes of acute alcoholism and alcoholic liver disease in Chinese.

At present, the anti-alcoholism drugs on the market can be mainly classified into three categories according to the efficacy.

The first is an alcohol absorption inhibitor, which uses alcohol dehydrogenase as a main component to metabolize alcohol in the gastrointestinal tract. For example, the chinese patent application CN104984156A discloses a traditional Chinese medicine anti-alcohol preparation, which is composed of hawthorn, lotus leaf, mulberry leaf, dandelion, lophatherum gracile, duckweed and tangerine leaf, and can effectively accelerate alcohol metabolism, significantly reduce the alcohol concentration in blood after drinking a large amount of alcohol, achieve the purpose of anti-alcohol and sober up, and also can protect the organs of people such as kidney, liver and the like, and greatly reduce the harm of alcohol to human body. However, these anti-alcoholics are characterized by taking before drinking to reduce the amount of alcohol entering blood, and taking after drinking can not clear ethanol and its metabolite acetaldehyde.

The second type is a liver-protecting medicine, for example, Chinese patent application publication No. CN106806844A discloses an anti-hangover medicine, which is composed of six traditional Chinese medicines including 10 parts of rhizoma Atractylodis Macrocephalae, 15 parts of pericarpium Citri Reticulatae, 6 parts of green tea, 6 parts of ginger, 8 parts of honeysuckle flower and 9 parts of American ginseng. The beverage is prepared from pure natural raw materials, is safe and reliable, has no toxic or side effect, protects the liver and the kidney while relieving alcoholism, and is beneficial to body health. But it is mainly protective against the liver system and has no significant protective effect against other systems, such as the cardiovascular system.

The third type is an analeptic dosage form, and as disclosed in the patent document with the Chinese patent application publication No. CN106924687A, the oral traditional Chinese medicine for relieving alcoholism can promote the metabolism of alcohol in vivo, so that acetaldehyde generated by the metabolism of alcohol is quickly further metabolized into acetic acid, thereby alleviating the symptoms of drunkenness such as headache, dizziness, dysphoria, palpitation and the like; can also relieve the inhibiting effect of alcohol on central nervous system, and prevent dysfunction of circulatory system, respiratory system, and digestive system; the raw materials are cheap and easy to obtain, have no toxic or side effect and are suitable for most of people who drink. The medicine enters into organism to generate corticoid stress effect, and neutralizes the inhibition effect of alcohol on central nerve to achieve the aim of sobering up, but the anti-alcoholism medicine only treats the symptoms and the root causes, and can cause damage to the central nerve of human body after long-term use.

In addition, none of the three anti-hangover agents has the efficacy of treating alcohol allergy.

Meanwhile, diabetes mellitus in China becomes the 3 rd main disease (cardiovascular disease, tumor and diabetes mellitus) in non-infectious chronic diseases, is a common frequently encountered disease which seriously threatens the health of people in China, brings huge economic and psychological burden to patients, seriously influences the life quality of the patients, also brings heavy burden to national economy and seriously influences the development of social economy. The disease is fast in growth, the complications are many, the population is wide, the sanitary resource consumption is large, and the disability and the death are all the time at the eye striking pace. Therefore, the prevention and control of diabetes development is a major health problem that is urgently needed to be solved in our country and even all over the world.

Diabetes is known as diabetes in traditional Chinese medicine, and is clinically characterized by polydipsia, diuresis, polyphagia, emaciation, fatigue and urine sweetness, and the diseased parts are pancreas, stomach and kidney. Modern scholars mostly consider that the basic mechanism of diabetes is yin and fluid consumption and excessive dryness-heat. It is also considered that after long-term diabetes and out-of-control disease, yin deficiency involving yang, heat burning, blood stasis, qi and yin deficiency, yin-yang deficiency, collateral channel stasis, channel malnutrition, qi and blood disorder, viscera organ damage, carbuncle, vertigo, thoracic obstruction, deafness, blindness, limb numbness and pain, lower limb gangrene, renal failure, edema, stroke, coma and other complications occur.

The course of diabetes is slow, no specific medicine for curing diabetes exists at present, and natural plants with small toxic and side effects as much as possible are the key for treating diabetes.

For example, Chinese patent application CN102935186B discloses a health tea for preventing diabetes, which is prepared from 7 medicinal materials including 15-25 g of dandelion, 10-20 g of asparagus, 10-20 g of dwarf lilyturf tuber, 5-15 g of corn stigma, 15-25 g of trichosanthes root, 10-20 g of herba patriniae and 3-6 g of schizonepeta.

For another example, the chinese patent application CN103157013B discloses a herb tea for treating diabetes, which is prepared from 7 kinds of traditional Chinese medicines including 5-10 parts of kalimeris indica, 5-10 parts of plantain, 15-20 parts of cymbidium ensifolium, 10-15 parts of Chinese ladybell herb, 20-25 parts of crinis carbonisatus, 10-15 parts of snakegourd fruit and 20-25 parts of dried guava.

The health protection tea disclosed above has good curative effect for treating diabetes, but has the following disadvantages: firstly, the health-care tea has complex components, difficult quality control and long preparation process, and is not beneficial to the product quality and the stability of the curative effect; secondly, the health protection tea does not relate to deep scientific explanation of the efficacy.

Further, as disclosed in chinese patent application CN104910240A, a novel plant-derived drug for treating diabetes, namely triterpene saponin compounds 1-3, is obtained by alcohol extraction from flowers of photosperms. However, the extraction steps are complicated, and the water solubility is poor, so that the tea is not suitable for use in the form of health-care tea.

Therefore, the health-care tea with definite curative effect, simple components, low cost and obvious effects of dispelling the effects of alcohol, protecting the liver, reducing blood sugar and reducing blood fat is needed to be provided. Meanwhile, the preparation of the medicine which is in line with the characteristics of the Chinese physique, has comprehensive effects and few side effects, and has the functions of dispelling the effects of alcohol, protecting the liver, reducing the blood sugar and reducing the blood fat also has important social significance and economic value.

Disclosure of Invention

1. Problems to be solved

The invention aims to provide application of the photophyllanthus urinaria, and solves the problems that the application and development of the conventional photophyllanthus urinaria are incomplete, the conventional preparation process is long, the product quality and the stability of curative effect are not facilitated, and deep-level scientific explanation of the medicinal effect is not involved. The invention adopts high-temperature water decoction or water soaking to obtain the liquid containing the glabrous leafflower, or adopts the ultralow-temperature freeze drying technology to prepare the powder of the glabrous leafflower, which can more comprehensively and better utilize various water-soluble functional components in medicinal materials, so that the medicinal materials are easier to utilize and absorb by human bodies, the health care effect of the medicinal materials is fully exerted, and the invention has the advantages of simple components, simple and convenient manufacturing process, easy control of sanitary indexes and convenient wide popularization and application.

2. Technical scheme

In order to solve the problems, the technical scheme adopted by the invention is as follows:

application of glabrous leafflower in preparation of composition with liver protecting effect and/or anti-alcoholism effect is disclosed, wherein the application part of glabrous leafflower is flower and/or bract and/or branch and/or leaf of glabrous leafflower.

Use of flowers and/or bracts and/or branches and/or leaves of photoleaf flowers for preparing a composition having an effect of inhibiting elevation of glutamic-pyruvic transaminase and/or glutamic-oxalacetic transaminase.

Use of flowers and/or bracts and/or branches and/or leaves of photoleaf flowers for the preparation of a composition having an effect of increasing the activity of alcohol dehydrogenase and/or acetaldehyde dehydrogenase.

The flowers, bracts, branches and leaves of the photoleaf flowers of the invention can be selected from the flowers, bracts, branches and leaves before or after flowering.

The application part of the photophyllanthus is the flower and/or bract and/or branch and/or leaf of the photophyllanthus.

Application of flowers and/or bracts and/or branches and/or leaves of photospermum in preparation of composition with effects of relieving alcoholism, protecting liver and/or reducing blood sugar and blood lipid.

Further, the composition includes a pharmaceutical composition, a food composition, a nutraceutical composition, a tea product composition or a product containing flowers and/or bracts and/or branches and/or leaves of the photophyllum. The composition also comprises sucrose, and the sucrose is added to ensure that the sucrose content in the preparation is 10-20% (g/mL).

Furthermore, the composition also comprises an aromatic and a preservative, wherein the mass content of the aromatic is 0.2-1%, the mass content of the preservative is 0.01-0.5%, the aromatic comprises one or more of almond essence, strawberry essence, sweet orange essence, litchi essence, apple essence, milk essence, mango essence, lemon essence and chocolate essence, and the preservative comprises one or more of sorbic acid, potassium sorbate, sodium dehydroacetate, sodium lactate, ethyl hydroxybenzoate and sodium citrate.

Further, the composition also comprises a sweetening agent with the mass content of 0.2-25%, the sweetening agent is a common edible additive and comprises a high-intensity sweetening agent and a low-intensity sweetening agent, and the high-intensity sweetening agent is one or more of aspartame, stevioside, acesulfame potassium, sodium cyclamate and sucralose; the low sweetener is one or more of sorbitol, xylitol, fructose, honey, maltose, starch sugar, lactose and sucrose.

Further, the composition comprises the flowers and/or the bracts and/or the branches and/or the leaves of the photoleaf flowers and an acceptable carrier, wherein the flowers and/or the bracts and/or the branches and/or the leaves of the photoleaf flowers are active ingredients and account for 1-99% of the composition by weight.

Further, the flower and/or bract and/or branch and/or leaf of the glabrous leafflower are boiled by water to obtain boiling liquid for application, or soaked by water to obtain soaking liquid for application, or directly freeze-dried into powder for application.

Further, the flowers and/or bracts and/or branches and/or leaves of the photic leafflower are freeze-dried to be made into powder for use and/or direct use.

Further, the flower and/or bract and/or branch and/or leaf of the photoleaf flower is pulverized into powder with a particle size of 1mm or less.

Further, the method for processing the branches and leaves of the phoma japonica comprises the step of withering the flowers and/or the bracts and/or the branches and/or the leaves before boiling or soaking in water or freeze-drying into powder.

A composition with hangover relieving and/or liver protecting effects comprises flower and/or bract and/or branch and/or leaf of Rubus niveus flower as effective component.

Furthermore, the application form of the flowers and/or bracts and/or branches and/or leaves of the glabrous leafflower is to prepare tablets, dripping pills, capsules or injections with auxiliary materials.

The water extract or the medicinal powder is used as a medicament, which is a traditional use mode of the traditional Chinese medicine, after water extraction, most of effective components can be dissolved out due to the wide dissolution range of water, so that the medicament is easier to be absorbed by a human body and has quicker response, such as administration forms of decoction and the like; the powder has large surface area and is favorable for the absorption of effective components in the medicinal materials in vivo. The medicinal materials are not extracted, the effective components still need to be dissolved out and absorbed in vivo, the effect is slower than that of an aqueous extract, but the toxic and side effects of harmful components in the medicinal materials on human bodies are weakened, and the medicinal materials are suitable for long-term administration, such as preparation of original powder into pills and other administration forms. In the pharmaceutical process, ethanol is used as a solvent to extract medicines at present, and is one of the most common extraction modes, ethanol is a medium-polarity solvent, the solubility performance of the ethanol is between that of a polar solvent and a non-polar solvent, some water-soluble ingredients can be dissolved, some ingredients dissolved by the non-polar solvent can also be dissolved, and the ethanol is usually used for extracting instead of decocting in water, so that the dissolution of a large amount of ineffective ingredients is avoided, the concentration and the extraction efficiency of effective ingredients are improved, the price of the ethanol is more expensive than that of water, and in the large production of the modern pharmaceutical industry, in order to save the production cost, the water is usually mainly decocted. In order to meet the requirements of various production and use, a specific dosage form can be prepared by water extraction, raw powder, alcohol extraction or a combination method of the water extraction, the raw powder and the alcohol extraction.

The compounds of the present invention may be prepared in suitable pharmaceutical forms using formulation techniques or pharmaceutical methods conventional in the art, including: tablets, injections, tinctures, suppositories, capsules, ointments (ointments, creams), pills, implants, syrups, mists (aerosols, powders, sprays), films, granules, oral solutions (oral suspensions, oral emulsions), powders, lotions (rinses, enemas), liniments (paints, plastics), gels, patches and the like; preferably tablets, capsules, ophthalmic preparations. Wherein the tablet is selected from buccal tablet, sublingual tablet, buccal patch, chewable tablet, dispersible tablet, soluble tablet, effervescent tablet, sustained release tablet, controlled release tablet, enteric coated tablet, etc.; the injection is selected from injection, transfusion, freeze-dried powder injection, emulsion, implant, microsphere preparation, pellet preparation and the like; the capsule is selected from hard capsule, soft capsule, sustained release capsule, controlled release capsule, enteric capsule, etc.; the pill is selected from dripping pill, sugar pill, etc.; the granules are selected from suspension granules, effervescent granules, enteric granules, sustained-release granules, controlled-release granules and the like.

The pharmaceutically acceptable carrier of the present invention refers to a substance contained in a dosage form in addition to the active ingredient, including but not limited to fillers (diluents), lubricants (glidants or anti-adherents), dispersants, wetting agents, binders, regulators, solubilizers, antioxidants, bacteriostats, emulsifiers, disintegrants, etc. The binder comprises syrup, acacia, gelatin, sorbitol, tragacanth, cellulose and its derivatives (such as microcrystalline cellulose, sodium carboxymethylcellulose, ethyl cellulose or hydroxypropyl methylcellulose), gelatin slurry, syrup, starch slurry or polyvinylpyrrolidone; the filler comprises lactose, sugar powder, dextrin, starch and its derivatives, cellulose and its derivatives, inorganic calcium salt (such as calcium sulfate, calcium phosphate, calcium hydrogen phosphate, precipitated calcium carbonate, etc.), sorbitol or glycine, etc.; the lubricant comprises superfine silica gel powder, magnesium stearate, talcum powder, aluminum hydroxide, boric acid, hydrogenated vegetable oil, polyethylene glycol and the like; the disintegrating agent comprises starch and its derivatives (such as sodium carboxymethyl starch, sodium starch glycolate, pregelatinized starch, modified starch, hydroxypropyl starch, corn starch, etc.), polyvinylpyrrolidone or microcrystalline cellulose, etc.; the wetting agent comprises sodium lauryl sulfate, water or alcohol, etc.; the antioxidant comprises sodium sulfite, sodium bisulfite, sodium pyrosulfite, dibutylbenzoic acid, etc.; the bacteriostatic agent comprises 0.5% of phenol, 0.3% of cresol, 0.5% of chlorobutanol and the like; the regulator comprises hydrochloric acid, citric acid, potassium (sodium) hydroxide sodium citrate, and buffer (including sodium dihydrogen phosphate and disodium hydrogen phosphate); the emulsifier comprises polysorbate-80, sorbitan fatty acid, pluronic F-68, lecithin, soybean lecithin, etc.; the solubilizer comprises Tween-80, bile, glycerol, etc.

The acceptable carrier component has certain physiological activity, but the addition of the component does not change the dominance of the compound or the derivative in the disease treatment process, but only plays an auxiliary effect, and the auxiliary effects are only the utilization of the known activity of the component and are auxiliary treatment modes which are conventional in the field of medicine. It is within the scope of the present invention to use the aforementioned auxiliary acceptable carrier ingredients in combination with the compounds of the present invention.

The invention can also be used as an additive to be added into various beverages and foods to prepare drinks, foods or health products with the functions of reducing blood sugar and blood fat, dispelling the effects of alcohol and protecting liver.

3. Advantageous effects

Compared with the prior art, the invention has the beneficial effects that:

(1) the product is prepared from the parts such as flowers, bracts, branches and leaves of the glabrous leafflower, has simple components, obvious effects of dispelling the effects of alcohol, protecting liver, reducing blood sugar and reducing blood fat, simple composition, easily controlled quality and simple preparation process, ensures the stability of the product quality, has no toxic or side effect on human bodies, has no drug resistance and can be taken for a long time.

(2) The invention adopts a high-temperature water decoction method and an ultralow-temperature freeze drying technology to prepare the application of the cotyledon aurea, can more comprehensively and better utilize various water-soluble functional components in medicinal materials, enables the medicinal materials to be more easily utilized and absorbed by human bodies, and fully exerts the health care effect of the medicinal materials.

(3) The withering method is adopted in the steps of preparing the euphorbia maculata flower, and aims to properly evaporate water, soften the leaf texture, facilitate rolling, promote the change of the contained substances along with the concentration of the leaf cells and the enhancement of enzyme activity caused by the water emission, and create conditions for forming the quality characteristics of the golden single clump.

(4) The prepared phyllanthus niruri can also treat alcohol allergy which is an external skin allergy symptom reaction caused by lack of acetaldehyde converting enzyme in vivo, and the invention can improve the activity of acetaldehyde dehydrogenase, help acetaldehyde converted by alcohol in allergic constitution to be decomposed into acetic acid and be discharged out of body, and avoid acetaldehyde poisoning and allergic symptoms.

(5) The invention develops the flowers and/or fresh branches and leaves of the photophyllum japonicum, has low cost, good social value and market value, and good development and application prospect.

(6) The method is designed according to test items, test principles and result judgment of an auxiliary blood sugar reducing function evaluation method and test items, test principles and result judgment of an auxiliary blood fat reducing function evaluation method, meets the evaluation and declaration requirements of CFDA health food, and reasonably evaluates the test items, the test principles and the result judgment of the auxiliary blood fat reducing function evaluation method by referring to the research literature of alcohol-relieving and liver-protecting.

Detailed Description

The present invention will be described in further detail with reference to the following examples. The present invention is described in terms of the preferred embodiment, but not limited to the embodiment, and any equivalent variations may be made by those skilled in the art using the teachings disclosed above. Any simple modifications or equivalent changes made to the following examples according to the technical essence of the present invention, without departing from the technical spirit of the present invention, fall within the scope of the present invention.

The invention is further described with reference to specific examples.

Example 1

The invention relates to a preparation method of photoleaf flowers

Application of branches and leaves of photophyllanthus

1. Preparing a boiling liquid:

picking and selecting raw leaves: selecting green leaf buds or young leaves without disease spots and insect pests, cleaning, removing dust and microorganisms on the leaf surfaces, draining water, and uniformly spreading in a ventilated and dry place;

withering: naturally withering for 3h, and turning over for 1 time until the leaves are withered and soft and the water loss rate is about 8%;

boiling: boiling the withered leaf of the glabrous leaf cotyledon and purified water according to the material ratio of 1:20 for 2 hours, collecting an extracting solution, boiling again according to the material ratio of 1:15 for 2 hours, and collecting the extracting solution; and (3) filtering: filtering the collected extractive solution with 12 layers of sterile gauze, and collecting the filtrate to obtain the decoction.

2. Preparing a hot water soaking solution:

soaking: soaking withered leaf of Photonic camellia nitidissima with 80 ℃ purified water according to the material ratio of 1:20 for 2h, collecting an extracting solution, boiling for 2h again according to the material ratio of 1:15, and collecting the extracting solution; and (3) filtering: filtering the collected extractive solution with 12 layers of sterile gauze, and collecting filtrate to obtain soaking solution;

3. preparing a normal-temperature soaking solution:

picking raw leaves, selecting tender green leaves or tender leaves without disease spots or insect pests, cleaning, removing dust and microorganisms on the surfaces of the leaves, draining water, and uniformly spreading in a ventilated and dry place; naturally withering for 3h, and turning over for 1 time until the leaves are withered and soft and the water loss rate is 8%; soaking withered leaf of the glabrous leaf cotyledon with purified water at room temperature for 48h according to the material ratio of 1:20, collecting the extracting solution, soaking at room temperature for 24h according to the material ratio of 1:15, and collecting the extracting solution to obtain a normal-temperature soaking liquid for later use.

4. Preparing a tea extract:

picking raw leaves, selecting tender green leaves or tender leaves without disease spots or insect pests, cleaning, removing dust and microorganisms on the surfaces of the leaves, draining water, and uniformly spreading in a ventilated and dry place; naturally withering for 3h, and turning over for 1 time until the leaves are withered and soft and the water loss rate is 8%; baking withered leaf of Photonic acid cotyledon, deactivating enzyme, soaking in pure boiled water at room temperature at a material ratio of 1:20 for 30min, collecting extractive solution, soaking in boiled water at a material ratio of 1:15 for 30min, and collecting extractive solution; filtering with gauze; carrying out suction filtration; and (5) carrying out rotary steaming at room temperature under reduced pressure until the extract is prepared into an extract state for later use.

5. Preparation of lyophilized powder

Picking raw leaves, selecting tender green leaves or tender leaves without disease spots or insect pests, cleaning, removing dust and microorganisms on the surfaces of the leaves, draining water, and uniformly spreading in a ventilated and dry place; naturally withering for 3h, and turning over for 1 time until the leaves are withered and soft and the water loss rate is 8%; subpackaging withered leaf of Ophiopogon japonicus, pre-freezing in-80 deg.C refrigerator for 4h, transferring into freeze dryer, freeze drying until water completely disappears, and taking out the freeze-dried product; micronizing the lyophilized product into superfine powder with particle diameter below 1 mm.

6. Sauce preparation

Picking raw leaves, selecting tender green leaves or tender leaves without disease spots or insect pests, cleaning, removing dust and microorganisms on the surfaces of the leaves, draining water, and uniformly spreading in a ventilated and dry place; naturally withering for 3h, and turning over for 1 time until the leaves are withered and soft and the water loss rate is 8%; micronizing withered leaf of Ophiopogon japonicus, and making into sauce with particle diameter below 1 mm.

Second, the extraction of bracts and flowers of the Ophiophyllum

1. Boiling preparation:

selecting bracts or flowers with bright color, no scab and no insect pest, cleaning, removing surface dust and microorganisms, draining water, and spreading uniformly in a ventilated and dry place; naturally withering for 3h, and turning over for 1 time until the bract or flower is withered and soft and the water loss rate is 8%; boiling the withered bracts and flowers with purified water according to the material ratio of 1:20 for 2h, collecting liquid, boiling again according to the material ratio of 1:15 for 2h, and collecting the boiling liquid of the glabrous leafflower for later use.

2. Preparing a hot water soaking solution:

selecting bracts or flowers with bright color, no scab and no insect pest, cleaning, removing leaf surface dust and microorganisms, draining water, and spreading uniformly in a ventilated and dry place;

withering: naturally withering for 3h, and turning over for 1 time until the bract or flower is withered and soft and the water loss rate is 8%;

soaking: soaking withered bracts and flowers of the glabrous leaf spica with 80 ℃ purified water according to the material ratio of 1:20 for 2h, collecting an extracting solution, boiling for 2h according to the material ratio of 1:15 again, and collecting the extracting solution; and (3) filtering: filtering the collected extractive solution with 12 layers of sterile gauze, and collecting filtrate to obtain soaking solution;

3. preparing a normal-temperature soaking solution:

selecting bracts or flowers with bright color, no scab and no insect pest, cleaning, removing leaf surface dust and microorganisms, draining water, and spreading uniformly in a ventilated and dry place; naturally withering for 3h, and turning over for 1 time until the bract or flower is withered and soft and the water loss rate is 8%; soaking withered bracts or flowers of the glabrous leaf spica and purified water at room temperature for 48h according to the material ratio of 1:20, collecting the extracting solution, soaking at room temperature for 24h according to the material ratio of 1:15, and collecting the extracting solution to obtain a normal-temperature soaking liquid for later use.

4. Preparing a tea extract:

selecting bracts or flowers with bright color, no scab and no insect pest, cleaning, removing leaf surface dust and microorganisms, draining water, and spreading uniformly in a ventilated and dry place; naturally withering for 3h, and turning over for 1 time until the bract or flower is withered and soft and the water loss rate is 8%; baking withered bracts or flowers of the glabrous leaf spicebush, deactivating enzymes, soaking in pure boiled water at room temperature for 30min according to the material ratio of 1:20, collecting the extract, brewing with boiled water at the material ratio of 1:15 for 30min, collecting the extract, filtering with gauze, performing suction filtration, and performing rotary steaming at room temperature under reduced pressure to obtain an extract for later use.

5. Preparation of lyophilized powder

Selecting bracts or flowers with bright color, no scab and no insect pest, cleaning, removing leaf surface dust and microorganisms, draining water, and spreading uniformly in a ventilated and dry place; naturally withering for 3h, and turning over for 1 time until the leaves are withered and soft and the water loss rate is 8%; subpackaging withered bracts or flowers of the Ophiopogon japonicus, pre-freezing in a refrigerator at-80 deg.C for 4h, transferring into a freeze-drying machine, freeze-drying until water completely disappears, and taking out the freeze-dried product; micronizing the lyophilized product into superfine powder with particle diameter below 1 mm.

6. Sauce preparation

Selecting bracts or flowers with bright color, no scab and no insect pest, cleaning, removing leaf surface dust and microorganisms, draining water, and spreading uniformly in a ventilated and dry place; naturally withering for 3h, and turning over for 1 time until the bract or flower is withered and soft and the water loss rate is 8%; micronizing withered bracts or flowers of Ophiopogon japonicus, and making into sauce with particle diameter below 1 mm.

Example 2

The invention discloses a research on the effects of relieving alcoholism and protecting liver

Materials (I) and (II)

1. Laboratory animal

SPF level male Kunming mouse, the quality of health is 18 ~ 20g, is provided by Yangzhou university's comparative medical center, and the certification number: SCXK (su) 2017-.

2. Medicaments and agents

Boiling liquid of branches and leaves of the glabrous leafflower: (see example 1 for detailed preparation). In the experiment, sterile water is prepared into solution with corresponding concentration for the intragastric administration of the mice. The administration dose of the mouse is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day of a 70kg adult in terms of body surface area.

Hot water soaking solution of branches and leaves of the cotyledon euphorbia: (see example 1 for detailed preparation). In the experiment, sterile water is prepared into solution with corresponding concentration for the intragastric administration of the mice. The administration dose of the mouse is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day of a 70kg adult in terms of body surface area.

Normal-temperature soaking solution of branches and leaves of photorhabdus japonica: (see example 1 for detailed preparation). In the experiment, sterile water is prepared into solution with corresponding concentration for the intragastric administration of the mice. The administration dose of the mouse is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day of a 70kg adult in terms of body surface area.

Bare leaf, flower branch and leaf tea: (see example 1 for detailed preparation). In the experiment, sterile water is prepared into solution with corresponding concentration for the intragastric administration of the mice. The administration dose of the mouse is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day of a 70kg adult in terms of body surface area.

Freeze-dried powder of branches and leaves of photorhabdus japonica: (see example 1 for detailed preparation). In the experiment, sterile water is prepared into solution with corresponding concentration for the intragastric administration of the mice. The administration dose of the mouse is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day of a 70kg adult in terms of body surface area.

Guanyezi flower, branch and leaf sauce: (see example 1 for detailed preparation). In the experiment, sterile water is prepared into solution with corresponding concentration for the intragastric administration of the mice. The administration dose of the mouse is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day of a 70kg adult in terms of body surface area.

The bud and the boiling liquid of the glabrous leaf bud and the flower: (see example 1 for detailed preparation). In the experiment, sterile water is prepared into solution with corresponding concentration for the intragastric administration of the mice. The administration dose of the mouse is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day of a 70kg adult in terms of body surface area.

Soaking the buds and flowers of the glabrous leaf in hot water: (see example 1 for detailed preparation). In the experiment, sterile water is prepared into solution with corresponding concentration for the intragastric administration of the mice. The administration dose of the mouse is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day of a 70kg adult in terms of body surface area.

Soaking the bracts and flowers of the glabrous leaf at normal temperature: (see example 1 for detailed preparation). In the experiment, sterile water is prepared into solution with corresponding concentration for the intragastric administration of the mice. The administration dose of the mouse is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day of a 70kg adult in terms of body surface area.

Leafy buds and scented tea: (see example 1 for detailed preparation). In the experiment, sterile water is prepared into solution with corresponding concentration for the intragastric administration of the mice. The administration dose of the mouse is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day of a 70kg adult in terms of body surface area.

Crushing the buds and the lyophilized powder of the photospermum: (see example 1 for detailed preparation). In the experiment, sterile water is prepared into solution with corresponding concentration for the intragastric administration of the mice. The administration dose of the mouse is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day of a 70kg adult in terms of body surface area.

Cotyledon and flower paste: (see example 1 for detailed preparation). In the experiment, sterile water is prepared into solution with corresponding concentration for the intragastric administration of the mice. The administration dose of the mouse is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day of a 70kg adult in terms of body surface area.

Positive control (Pepp conscious chewable tablet): purchased from shandeshi company. Taking the sobering chewable tablets, grinding, and preparing a solution with corresponding concentration by using sterile water for gastric lavage of the mice. The mouse dose is 8320mg/kg, which is equivalent to 64 g/day of adult human and is equivalent to 8 times of the clinical equivalent dose according to the conversion of body surface area.

Big ox guy-Erguotou (ox guy mountain winery) of 56 degree ox guy mountain; absolute ethanol (Shanghai Tantake Technology Co., Ltd.); acetaldehyde (Chengdu Kelong chemical reagent plant); butanone (Baishi chemical Co., Tianjin); ethanol dehydrogenase and acetaldehyde dehydrogenase kit (Nanjing, Bioengineering Co., Ltd.); glutamic-pyruvic transaminase kit and glutamic-oxalacetic transaminase kit (Nanjing to build bioengineering Co., Ltd.); physiological saline (Anhui double crane pharmaceutical industry, Inc.).

3. Main instrument

Gas chromatograph: varian CP-3800; a workstation: CTC analytical; a chromatographic column: CP-Wax52CB, Cpsil5CB-2 capillary column; a detector: a FID detector; general UV spectrophotometer for Beijing UV-1 chromatography.

Second, method

The SPF-grade normal KM mice are randomly divided into 15 groups, namely a normal control group, a model control group, a positive control group, a glabrous leaf branch and leaf boiling liquid, a glabrous leaf branch and leaf hot water soaking liquid, a glabrous leaf branch and leaf normal-temperature soaking liquid, glabrous leaf branch and leaf tea, glabrous leaf branch and leaf freeze-dried crushed substances, glabrous leaf branch and leaf paste, glabrous leaf bud and leaf boiling liquid, glabrous leaf bud and flower hot water soaking liquid, glabrous leaf bud and flower normal-temperature soaking liquid, glabrous leaf bud and flower tea, glabrous leaf bud and flower freeze-dried powder fragments and glabrous leaf bud or flower paste, and 10 mice in each group are taken. The animals of each group were administered by gavage, and the normal control group and the model control group were given equal volume of sterile water for 1 time/day. After 1h of administration for each time, mice of other groups except the normal control group are subjected to intragastric administration according to 0.12mL/10g of the stomach, and a 56-degree Daizhanshan Erguotou is administered to cause acute alcoholic liver injury model, and continuous administration and model building are carried out for 6 d. After the last molding is carried out for 1.5h, 0.5-1 mL of blood is taken from each group of eyeballs, the eyeballs are centrifuged at 3000rpm for 10min to separate serum, a gas chromatograph is adopted to measure the concentration of ethanol and acetaldehyde in the serum, and the AST and ALT activities of the serum are measured according to the operation method of the kit. After the eyeballs are picked and blood is taken, the cervical vertebra is dislocated immediately to kill the mice, 0.5g of fresh liver is taken and added with precooled physiological saline solution to prepare 10% liver tissue homogenate, the homogenate is centrifuged at 3000rpm for 10min, and the supernatant is taken to determine the activity of ADH and ALDH.

The statistical processing method adopts statistical software, data is expressed by mean plus or minus standard deviation, the comparison among a plurality of groups adopts one-factor variance analysis, the comparison between every two groups is tested by LSD, and the difference is P less than 0.05, thus having statistical significance.

Three, result in

1. Effect of the invention on the concentration of ethanol and acetaldehyde in mouse serum

As can be seen from Table 1, compared with the normal control group, the concentrations of ethanol and acetaldehyde in the serum of the mice in the model control group are significantly increased, and the difference has statistical significance (p is less than 0.01), which indicates that the modeling method can successfully cause the significant increase of the concentrations of ethanol and acetaldehyde in the serum of the mice.

TABLE 1 mouse serum ethanol, acetaldehyde content

Note: p <0.01 compared to model control; p < 0.05; in comparison to the normal control group, # # p < 0.01.

Compared with a model control group, the concentration of ethanol and acetaldehyde in mouse serum of the glabrous leaf branch and leaf boiling liquid, the glabrous leaf branch and leaf hot water soaking liquid, the glabrous leaf branch and leaf normal-temperature soaking liquid, the glabrous leaf branch and leaf tea, the glabrous leaf branch and leaf freeze-dried crushed substance, the glabrous leaf branch and leaf paste and the positive drug group is remarkably reduced, and the difference has statistical significance (P is less than 0.01), so that the glabrous leaf branch and leaf boiling liquid, the glabrous leaf branch and leaf hot water soaking liquid, the glabrous leaf branch and leaf normal-temperature soaking liquid, the glabrous leaf branch and leaf tea, the glabrous leaf branch and leaf freeze-dried crushed substance, the glabrous leaf branch and leaf paste and the positive control group can remarkably inhibit the absorption of ethanol or promote the metabolism of ethanol and reduce the accumulation of acetaldehyde. Compared with a model control group, the mouse serum of the glabrous leaf bud and flower boiling liquid, the glabrous leaf bud and flower hot water soaking liquid, the glabrous leaf bud and flower normal-temperature soaking liquid, the glabrous leaf bud and flower tea, the glabrous leaf bud and flower freeze-dried crushed substance and the glabrous leaf bud and flower paste has obviously reduced concentration of ethanol and acetaldehyde, and the difference has statistical significance (P is less than 0.05), so that the glabrous leaf bud and flower boiling liquid, the glabrous leaf bud and flower hot water soaking liquid, the glabrous leaf bud and flower normal-temperature soaking liquid, the glabrous leaf bud and flower tea, the glabrous leaf bud and flower freeze-dried crushed substance and the glabrous leaf bud and flower paste can obviously inhibit absorption of ethanol or promote metabolism of ethanol and reduce accumulation of acetaldehyde.

Compared with the group of the invention, the concentration of ethanol and acetaldehyde in the serum of the mice of the positive control group is not obviously different from that of the group of the invention, which shows that the invention is equivalent to the positive control in the aspects of inhibiting ethanol absorption or promoting ethanol metabolism and reducing the accumulation of acetaldehyde.

2. The effect of the invention on the activity of AST (glutamic-oxalacetic transaminase) and ALT (glutamic-pyruvic transaminase) in serum of mice

As can be seen from Table 2, compared with the normal control group, the mouse serum AST and ALT activities of the model control group are significantly increased, and the difference has statistical significance (P is less than 0.01), which indicates that the modeling method can successfully cause the liver function damage of the mouse.

TABLE 2 AST and ALT Activity in mouse serum

Note: p <0.01 compared to model control; in comparison to the normal control group, # # p < 0.01.

Compared with a model control group, the AST and ALT activities in mouse serum of the glabrous leaf branch and leaf boiling liquid group, the glabrous leaf branch and leaf hot water soaking liquid, the glabrous leaf branch and leaf normal-temperature soaking liquid, the glabrous leaf branch and leaf tea, the glabrous leaf branch and leaf freeze-dried crushed substance and the glabrous leaf branch and leaf paste are remarkably reduced, and the differences have statistical significance (P is less than 0.01), so that the glabrous leaf branch and leaf boiling liquid, the glabrous leaf branch and leaf hot water soaking liquid, the glabrous leaf branch and leaf normal-temperature soaking liquid, the glabrous leaf branch and leaf tea, the glabrous leaf branch and leaf freeze-dried crushed substance and the glabrous leaf branch and leaf paste can remarkably inhibit liver function damage caused by alcohol. Compared with a model control group, the mouse serum AST and ALT activity of the glabrous leaf bud and flower boiling liquid, the glabrous leaf bud and flower hot water soaking liquid, the glabrous leaf bud and flower normal-temperature soaking liquid, the glabrous leaf bud and flower tea, the glabrous leaf bud and flower freeze-dried crushed substance and the glabrous leaf bud and flower paste is remarkably reduced, the difference has statistical significance (P is less than 0.05), and the glabrous leaf bud and flower boiling liquid, the glabrous leaf bud and flower hot water soaking liquid, the glabrous leaf bud and flower normal-temperature soaking liquid, the glabrous leaf bud and flower tea, the glabrous leaf bud and flower freeze-dried substance and flower crushed substance and the glabrous leaf bud and flower paste can remarkably inhibit damage caused by alcohol.

Compared with a model control group, the AST and ALT activity in the serum of the mice of the photophyllanthus roseus is reduced; compared with a positive control group, the effect of the photophyllanthus has no significant difference. Therefore, the branches, leaves, bracts and flowers of the cotyledon ladysthum can reduce the AST and ALT activity in the blood serum of the mouse, namely inhibit the abnormal increase of the AST and ALT in the blood serum of the mouse caused by alcohol and inhibit the liver function damage caused by the alcohol.

3. Effect of the present invention on ADH (alcohol dehydrogenase) and ALDH (aldehyde dehydrogenase) Activity in mouse liver

As can be seen from Table 3, compared with the normal control group, the ADH and ALDH activities in the liver of the mouse in the model control group are significantly reduced, and the difference has statistical significance, which indicates that the modeling method can successfully cause the reduction of the metabolic capability of the liver of the mouse to ethanol and acetaldehyde.

Compared with a model control group, the mouse livers of the glabrous leaf branch and leaf boiling liquid, the glabrous leaf branch and leaf hot water soaking liquid, the glabrous leaf branch and leaf normal-temperature soaking liquid, the glabrous leaf branch and leaf tea, the glabrous leaf branch and leaf freeze-dried crushed substance and the glabrous leaf branch and leaf sauce group have obviously improved ADH and ALDH activities, and the difference has statistical significance (P is less than 0.01). Compared with a model control group, the differences of the ADH and ALDH activities in the liver of mice with the photophyllous bud, flower boiling liquid, the photophyllous bud, flower hot water soaking liquid, the photophyllous bud, flower normal-temperature soaking liquid, the photophyllous bud, flower scented tea, the photophyllous bud, flower freeze-dried crushed substance and the photophyllous bud, flower sauce from the model control group are obviously increased, the statistical significance (P is less than 0.05) is achieved, and the photophyllous bud, flower boiling liquid, the photophyllous bud, flower hot water soaking liquid, the photophyllous bud, flower normal-temperature soaking liquid, the photophyllous bud, flower scented tea, the photophyllous bud, flower crushed freeze-dried substance and the photophyllous bud and flower sauce can obviously improve the ADH and ALDH activities in the liver and promote the metabolism of the liver on ethanol and acetaldehyde.

Compared with the photophyllum branch and leaf boiling liquid group, the mouse liver neutralization activity of the positive control group is obviously lower, and the difference has statistical significance (P is less than 0.05), which indicates that the photophyllum branch and leaf of the invention is superior to the positive control in the aspects of improving the ADH and ALDH activity in the liver and promoting the metabolism of the liver to ethanol and acetaldehyde.

TABLE 3 ADH and ALDH activity in liver tissue of mice

Note: p <0.01 compared to model control; compared with the normal control group, # p < 0.01; compared with the photoleaf flower branch and leaf boiling liquid, the electric discharge chemical has the electric discharge rate of p less than 0.05.

Fourthly, summarize

In this example, the branches, bracts and flowers of photonics flowers according to the present invention significantly inhibit the absorption of ethanol or promote the metabolism of ethanol, and reduce the accumulation of acetaldehyde, and the branches, bracts and flowers of photonics flowers according to the present invention are comparable to the positive control in terms of the inhibition of the absorption of ethanol or the promotion of the metabolism of ethanol, and the reduction of the accumulation of acetaldehyde. The branches, leaves, bracts and flowers of the photoleaf flowers can obviously inhibit the liver function damage caused by alcohol, and the photoleaf flowers are superior to positive controls in the aspect of inhibiting the liver function damage caused by alcohol. The branches, leaves, bracts and flowers of the Photonic flower can obviously improve the activity of liver ADH and ALDH and promote the metabolism of liver to ethanol and acetaldehyde, and are superior to the branches, leaves, bracts and flowers of the Photonic flower and positive control in improving the activity of liver ADH and ALDH and promoting the metabolism of liver to ethanol and acetaldehyde.

Example 3

The study on the activity of reducing blood sugar and blood fat

The first test method comprises the following steps:

animal feeding

Taking 8 male C57BL/6 mice as normal control group, taking a plurality of dbdb male mice with 7-8 weeks old for adaptive breeding for one week, fasting for 12h, cutting tails and taking blood, measuring fasting blood glucose by a glucometer, and selecting the dbdb mice with the fasting blood glucose more than or equal to 11.1mmol/L as type II diabetes mellitus mice.

Second, the animals are administered in groups

The modeled dbdb mice were divided into 15 groups: a model control group, a positive control group (pioglitazone hydrochloride tablets, Jiangsu Deyuan pharmaceutical industry Co., Ltd.), the glabrous leaf branch and leaf boiling liquid (750mg crude drug/kg), the glabrous leaf branch and leaf hot water soak solution (750mg crude drug/kg), the glabrous leaf branch and leaf normal temperature soak solution (750mg crude drug/kg), the glabrous leaf branch and leaf tea (750mg crude drug/kg), the glabrous leaf branch and leaf freeze-dried crushed substance (750mg crude drug/kg), the glabrous leaf branch and leaf paste (750mg crude drug/kg), the glabrous leaf bud and flower boiling liquid (750mg crude drug/kg), the glabrous leaf bud and flower hot water soak solution (750mg crude drug/kg), the glabrous leaf bud and flower normal temperature soak solution (750mg crude drug/kg), the invention relates to the bract and scented tea of the dodder (750mg crude drug/kg), the freeze-dried and crushed bract and flower of the dodder (750mg crude drug/kg), the bract and flower paste of the dodder (750mg crude drug/kg), and 8 plants. The animals of each group were administered by gavage once a day and fed with normal feed. The administration was continued for 7 weeks. The normal control group was fed with the same sterile water as the normal control group. The weight of each group of mice is detected every day, each group of mice is initially tested for oral glucose tolerance in the seventh week, eye sockets are sampled at the end of the seventh week, a glucometer is used for measuring blood glucose, whole blood is centrifuged to obtain serum, a mouse glycated serum albumin ELISA kit is used for measuring the glycated serum albumin (GA) value of each group of mice, a mouse insulin ELISA kit is used for measuring the insulin value of each group of mice, and the serum is sent to a hospital combining traditional Chinese medicine and western medicine in Jiangsu province for measuring related indexes of blood fat. After blood sampling, the neck of each group of mice is dislocated and killed, and the liver, the kidney, the spleen and the pancreas are dissected and weighed.

Third, statistical method

Each index is expressed as mean plus or minus standard deviation (x plus or minus s), and the significance test of the difference between two groups of means adopts a t test method.

In a spontaneous type II diabetes dbdb mouse animal model, positive drugs (pioglitazone hydrochloride tablets) and tabellae photorhagades of a gastric lavage model mouse are respectively injected at normal temperature and different doses of the tabellae photorhagades, the change of fasting blood glucose, lipid metabolism indexes, insulin and glycated serum albumin of a normal mouse and mice of each administration group are counted, and the results are shown in tables 4 to 7, wherein # p is less than 0.05, # p is less than 0.01, and # p is less than 0.001 in comparison of normal control groups; p <0.05, p <0.01, p <0.001, compared to the model control group.

Fourth, experimental results

1. Influence of water on blood sugar level of type II diabetic mice

As shown in Table 4, in fasting blood glucose measurement, compared with the normal control group, the blood glucose of the mice in the model control group is obviously increased, and the difference has statistical significance (P is less than 0.001), which indicates that the model of the type II diabetes mellitus hyperglycemia of the dbdb mice is successful. Compared with a model control group, the positive control group has obviously reduced fasting blood glucose after 49 days of administration, the difference has statistical significance (p is less than 0.01), the positive control group has obviously reduced fasting blood glucose after 49 days of administration, the photoleaf branch and leaf boiling liquid, the photoleaf branch and leaf normal-temperature soaking liquid, the photoleaf branch and leaf tea, the photoleaf branch and leaf freeze-dried crushed product and the photoleaf branch and leaf sauce have obviously reduced fasting blood glucose after 49 days of administration, the difference has statistical significance (p is less than 0.05), the photoleaf bud and flower boiling liquid, the photoleaf bud and flower hot-water soaking liquid, the photoleaf bud and flower normal-temperature soaking liquid, the photoleaf bud and flower tea, the photoleaf bud and flower freeze-dried crushed product, the photoleaf bud and flower sauce slightly reduce but do not have obvious difference, and the statistical significance (p is more than 0.05) shows that the photoleaf branch and leaf boiling liquid, the photoleaf branch and leaf boiling liquid and flower sauce of the invention have slightly reduced but not obvious difference, The normal-temperature soaking solution for the glabrous leafflower branches and leaves, the glabrous leafflower branch and leaf tea, the lyophilized and crushed glabrous leafflower branches and leaves, the glabrous leafflower branch and leaf sauce and the positive control can obviously reduce the blood sugar level of mice with type II diabetes, and the glabrous leafflower bud and flower bud boiling solution, the glabrous leafflower bud and flower hot-water soaking solution, the glabrous leafflower bud and flower normal-temperature soaking solution, the glabrous leafflower bud and flower tea, the glabrous leafflower bud and flower lyophilized and crushed substances, the glabrous leafflower bud and flower sauce can not obviously reduce the blood sugar level of mice with type II diabetes.

2. The influence of water on the glucose tolerance of type II diabetic mice

As shown in Table 4, in the measurement of glucose tolerance, compared with the normal control group, the area under the glucose tolerance curve (AUC) of the mice in the model control group is obviously increased, and the difference has statistical significance (P is less than 0.001), which indicates that the model of the type II diabetes mellitus hyperglycemia of the dbdb mice succeeds.

TABLE 4 blood glucose levels and glucose tolerance in type II diabetic mice administered at week seven

P <0.05, p <0.01, p <0.001, compared to the model control group, # p <0.05, # p <0.01, # p <0.001 compared to the normal control group, # p <0.001

Compared with a model control group, the AUC of the positive control group is remarkably reduced, the difference has statistical significance (p is less than 0.001), the AUC of the photoleaf branch and leaf boiling liquid, the photoleaf branch and leaf hot water soaking liquid, the photoleaf branch and leaf tea, the photoleaf branch and leaf freeze-dried crushed matter and the photoleaf branch and leaf sauce group is remarkably reduced, and the difference has statistical significance (p is less than 0.01), so that the photoleaf branch and leaf boiling liquid, the photoleaf branch and leaf hot water soaking liquid, the photoleaf branch and leaf tea, the photoleaf branch and leaf freeze-dried crushed matter and the photoleaf branch and leaf sauce group can remarkably relieve the glucose tolerance of a type II diabetic mouse, and further improve the diabetic symptom of the type II diabetic mouse; the normal-temperature soak solution AUC of the branches and leaves of the cotyledons tabacum is remarkably reduced, the difference has statistical significance (p is less than 0.05), and the normal-temperature soak solution of the branches and leaves of the cotyledons tabacum can also remarkably relieve the glucose tolerance of mice with type II diabetes, so that the symptoms of the diabetes of the mice with type II diabetes are improved; the method is characterized in that the Ophiopogon japonicus bract and flower boiling liquid, the Ophiopogon japonicus bract and flower hot water soaking liquid, the Ophiopogon japonicus bract and flower normal temperature soaking liquid, the Ophiopogon japonicus bract and flower tea, the Ophiopogon japonicus bract and flower freeze-dried crushed matter, the Ophiopogon japonicus bract and flower sauce group AUC are not obviously reduced, and the difference is not statistically significant (p is more than 0.05), so that the Ophiopogon japonicus bract and flower boiling liquid, the Ophiopogon japonicus bract and flower hot water soaking liquid, the Ophiopogon japonicus bract and flower normal temperature soaking liquid, the Ophiopogon japonicus bract and flower tea, the Ophiopogon japonicus bract and flower freeze-dried crushed matter, and the Ophiopogon japonicus bract and flower sauce group cannot obviously relieve the glucose tolerance of type II diabetes mice.

3. The influence of the water on the hyperlipidemia of the type II diabetic mice

As shown in Table 5, in the measurement of serum Triglyceride (TG), serum Total Cholesterol (TCHO), high density lipoprotein (HDL-C) and low density lipoprotein (LDL-C), compared with the normal control group, the serum TG, TCHO and LDL-C in the mouse of the model control group are obviously increased, and the differences have statistical significance (p is less than 0.001), which indicates that the hyperlipemia model of the mouse type II diabetes is successful. Compared with a model control group, TG and TCHO in serum of a positive control group mouse are obviously reduced, and the difference has statistical significance (p is less than 0.001), so that the positive control group can obviously improve the hyperlipemia of a type II diabetic mouse; the photophyllum branch and leaf boiling liquid, the photophyllum branch and leaf hot water soaking liquid, the photophyllum branch and leaf normal-temperature soaking liquid, the photophyllum branch and leaf tea, the photophyllum branch and leaf freeze-dried crushed substance and the photophyllum branch and leaf sauce group mouse serum TG, TCHO and LDL-C are all obviously reduced, and the difference has statistical significance (p is less than 0.001), so that the photophyllum branch and leaf boiling liquid, the photophyllum branch and leaf hot water soaking liquid, the photophyllum branch and leaf normal-temperature soaking liquid, the photophyllum branch and leaf tea, the photophyllum branch and leaf freeze-dried crushed substance and the photophyllum branch and leaf sauce group can obviously improve the hyperlipidemia symptom of type II diabetic mice; the invention discloses a photoleaf flower bud and flower boiling liquid, a photoleaf flower bud and flower hot water soaking liquid, a photoleaf flower bud and flower normal-temperature soaking liquid, a photoleaf flower bud and flower tea, a photoleaf flower bud and flower freeze-dried crushed substance, a photoleaf flower bud and flower sauce group mouse serum TG, TCHO and LDL-C are not obviously reduced, the difference has no statistical significance (p is more than 0.05), and the photoleaf flower bud and flower boiling liquid, the photoleaf flower bud and flower hot water soaking liquid, the photoleaf flower bud and flower normal-temperature soaking liquid, the photoleaf flower bud and flower tea, the photoleaf flower bud and flower freeze-dried crushed substance, the photoleaf flower bud and flower sauce group can not obviously improve the hyperlipidemia symptom of a type II diabetic mouse.

TABLE 5 serum TG, TCHO, HDL and LDL levels in type II diabetic mice administered at week seven

4. The Effect of the invention on glycated serum albumin in the serum of type II diabetic mice

As shown in Table 6, in the measurement of glycated serum albumin, the GA content in the serum of the mice in the model control group is obviously increased compared with that in the normal control group, and the difference has statistical significance (p is less than 0.01), which indicates the success of the mice with type II diabetes. Compared with a model control group, GA in the serum of a positive control group mouse is remarkably reduced, the difference has statistical significance (p is less than 0.01), GA in the serum of a photoleaf branch and leaf boiling liquid, a photoleaf branch and leaf hot water soaking liquid, a photoleaf branch and leaf normal-temperature soaking liquid, photoleaf branch and leaf tea, a photoleaf branch and leaf freeze-dried crushed substance and a photoleaf branch and leaf paste group mouse are remarkably reduced, and the difference has statistical significance (p is less than 0.01), so that the photoleaf branch and leaf boiling liquid, the photoleaf branch and leaf hot water soaking liquid, the photoleaf branch and leaf normal-temperature soaking liquid, the photoleaf branch and leaf tea, the photoleaf branch and leaf freeze-dried crushed substance and the photoleaf branch and leaf paste group can remarkably reduce the glycated albumin level of a type II diabetes mouse and improve the type II diabetes symptom of the mouse; and the glabrous leaf bud bract and flower boiling liquid, the glabrous leaf bud bract and flower hot water soaking liquid, the glabrous leaf bud bract and flower normal-temperature soaking liquid, the glabrous leaf flower bract and flower tea, the glabrous leaf bud bract and flower freeze-dried crushed matter, the glabrous leaf bud bract and flower sauce group mouse serum GA is not obviously reduced, the difference has no statistical significance (p is more than 0.05), and the differences show that the glabrous leaf bud bract and flower boiling liquid, the glabrous leaf bud bract and flower hot water soaking liquid, the glabrous leaf bud bract and flower normal-temperature soaking liquid, the glabrous leaf bud bract and flower tea, the glabrous leaf bud bract and flower freeze-dried crushed matter, the glabrous leaf bud bract and flower sauce group do not have the function of improving the mouse II-type diabetes symptoms.

TABLE 6 content of GA, INS and HOMA-IR in serum of type II diabetic mice at the seventh week of administration

5. The invention has the effects on insulin in the serum of a type II diabetic mouse and insulin resistance

As shown in Table 6, in the measurement of serum Insulin (INS) and insulin index (HOMA-IR), compared with the normal control group, the INS and insulin index of the model control group are obviously increased, and the difference has statistical significance (p is less than 0.01 or p is less than 0.001), which indicates that the mouse model with type II diabetes is successful. Compared with a model control group, INS and HOMA-IR of the positive control group are remarkably reduced, the difference has statistical significance (p is less than 0.05), and the positive control can remarkably reduce the level of insulin in the serum of a type II diabetic mouse and improve the insulin resistance of the type II diabetic mouse. The invention discloses a photophyllum branch and leaf boiling liquid, a photophyllum branch and leaf hot water soaking liquid, a photophyllum branch and leaf normal-temperature soaking liquid, photophyllum branch and leaf tea, a photophyllum branch and leaf freeze-dried crushed product, and a photophyllum branch and leaf sauce group mouse serum INS and HOMA-IR which are all obviously reduced, wherein the difference has statistical significance (p is less than 0.05), so that the photophyllum branch and leaf boiling liquid group, the photophyllum branch and leaf hot water soaking, the photophyllum branch and leaf normal-temperature soaking liquid, the photophyllum branch and leaf tea, the photophyllum branch and leaf freeze-dried crushed product and the photophyllum branch and leaf sauce group can obviously reduce the insulin level in the serum of a type II diabetic mouse and improve insulin resistance of the type II diabetic mouse. And the glabrous leaf bud bract and flower boiling liquid, the glabrous leaf bud bract and flower hot water soaking liquid, the glabrous leaf bud bract and flower normal-temperature soaking liquid, the glabrous leaf bud bract and flower tea, the glabrous leaf bud bract and flower freeze-dried crushed matter, INS and HOMA-IR in the blood serum of mice in the glabrous leaf bud bract and flower paste group are not obviously reduced, and the difference has no statistical significance (p is more than 0.05), so that the glabrous leaf bud bract and flower boiling liquid, the glabrous leaf bud bract and flower hot water soaking liquid, the glabrous leaf bud bract and flower normal-temperature soaking liquid, the glabrous leaf bud bract and flower tea, the glabrous leaf bud bract and flower freeze-dried crushed matter, the glabrous leaf bud bract and flower paste group can not obviously reduce the insulin level in the blood serum of mice with type II diabetes and improve the insulin resistance of the mice with type II diabetes. Note: insulin resistance index (HOMA-IR) — (insulin concentration x last fasting blood glucose value)/22.5.

6. The invention has the influence on the visceral organ indexes of liver, kidney and spleen of type II diabetic mice

In the measurement of liver, kidney and spleen indexes (see table 7), compared with the model group, the liver, kidney and spleen indexes of the type II diabetes mice of each administration group are not significantly changed, and the difference is not statistically significant (p is more than 0.05), which indicates that the branches, bracts and flowers of the photoleaf flowers do not generate significant side effects on the liver, kidney and spleen of the type II diabetes mice.

TABLE 7 weight and liver, spleen, and kidney indices of type II diabetic mice administered at week seven

P <0.05, p <0.01, p <0.001, compared to the model control group, # p <0.05, # p <0.01, # p <0.001 compared to the normal control group

Fifth, summarize

The results of this example show that the photophyllanthus flower branch and leaf boiling solution, the photophyllanthus flower branch and leaf hot water soaking solution, the photophyllanthus flower branch and leaf normal temperature soaking solution, the photophyllanthus flower branch and leaf tea, the photophyllanthus flower branch and leaf freeze-dried crushed product and the photophyllanthus flower branch and leaf sauce group of the present invention can all significantly reduce fasting blood glucose and serum albumin levels in type II diabetic mice, and improve oral glucose tolerance of type II diabetic mice, and meanwhile, the photophyllanthus flower branch and leaf boiling solution, the photophyllanthus flower branch and leaf hot water soaking solution, the photophyllanthus flower branch and leaf normal temperature soaking solution, the photophyllanthus flower branch and leaf tea, the photophyllanthus flower branch and leaf freeze-dried crushed product and the photophyllanthus flower branch and leaf sauce group of the present invention can also significantly reduce insulin levels, and alleviate insulin resistance symptoms of type II diabetic mice, and finally, the photophyllanthus flower branch and leaf boiling solution, the photophyllanthus flower branch and leaf hot water soaking solution, The Ligustrum japonicum branch leaf tea, the lyophilized and crushed Ligustrum japonicum branch leaves and the Ligustrum japonicum branch leaf sauce group can also obviously reduce the content of triglyceride, total cholesterol and low-density lipoprotein in the serum of a type II diabetic mouse, and have an obvious blood fat reducing effect on the type II diabetic mouse, and the invention has a certain regulation effect on glycolipid metabolism and an obvious blood sugar and blood fat reducing effect.

Example 4

The invention discloses research for preventing liver injury caused by carbon tetrachloride

Materials (I) and (II)

1. Laboratory animal

SPF grade SD rat, quality of health 150 ~ 180g, provided by Yangzhou university's comparative medical center, the certification number: SCXK (Su) 2017-.

2. Medicaments and agents

Boiling liquid of branches and leaves of the glabrous leafflower: (see example 1 for detailed preparation). During the experiment, pure water is prepared into solution with corresponding concentration for the intragastric administration of rats. The dosage of rat drug administration is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day of 70kg adult human body in terms of body surface area.

Hot water soaking solution of branches and leaves of the cotyledon euphorbia: (see example 1 for detailed preparation). During the experiment, pure water is prepared into solution with corresponding concentration for the intragastric administration of rats. The dosage of rat drug administration is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day of 70kg adult human body in terms of body surface area.

Normal-temperature soaking solution of branches and leaves of photorhabdus japonica: (see example 1 for detailed preparation). During the experiment, pure water is prepared into solution with corresponding concentration for the intragastric administration of rats. The dosage of rat administration is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day for 70kg adult based on body surface area conversion.

Bare leaf, flower branch and leaf tea: (see example 1 for detailed preparation). During the experiment, pure water is prepared into solution with corresponding concentration for the intragastric administration of rats. The dosage of rat administration is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day for 70kg adult based on body surface area conversion.

Freeze-dried powder of branches and leaves of photorhabdus japonica: (see example 1 for detailed preparation). During the experiment, pure water is prepared into solution with corresponding concentration for the intragastric administration of rats. The dosage of rat administration is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day for 70kg adult based on body surface area conversion.

Guanyezi flower, branch and leaf sauce: (see example 1 for detailed preparation). During the experiment, pure water is prepared into solution with corresponding concentration for the intragastric administration of rats. The dosage of rat administration is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day for 70kg adult based on body surface area conversion.

The bud or the boiling liquid of the glabrous leaf bud or the flower: (see example 1 for detailed preparation). During the experiment, pure water is prepared into solution with corresponding concentration for the intragastric administration of rats. The dosage of rat administration is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day for 70kg adult based on body surface area conversion.

Soaking the buds or flowers of the glabrous leaf in hot water: (see example 1 for detailed preparation). During the experiment, pure water is prepared into solution with corresponding concentration for the intragastric administration of rats. The dosage of rat administration is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day for 70kg adult based on body surface area conversion.

Normal-temperature soaking solution of bracts or flowers of glabrous leaf buds: (see example 1 for detailed preparation). During the experiment, pure water is prepared into solution with corresponding concentration for the intragastric administration of rats. The dosage of rat administration is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day for 70kg adult based on body surface area conversion.

Leafy bud or scented tea: (see example 1 for detailed preparation). During the experiment, pure water is prepared into solution with corresponding concentration for the intragastric administration of rats. The dosage of rat administration is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day for 70kg adult based on body surface area conversion.

Crushing the buds or the lyophilized powder of the photospermum: (see example 1 for detailed preparation). During the experiment, pure water is prepared into solution with corresponding concentration for the intragastric administration of rats. The dosage of rat administration is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day for 70kg adult based on body surface area conversion.

Cotyledon ladysthumb or flower paste: (see example 1 for detailed preparation). During the experiment, pure water is prepared into solution with corresponding concentration for the intragastric administration of rats. The dosage of rat administration is 750mg crude drug/kg, which is equivalent to 5.71g crude drug/day for 70kg adult based on body surface area conversion.

40％CCl₄The preparation of (1): 40mL of carbon tetrachloride is accurately measured and dissolved in 60mL of olive oil to prepare a carbon tetrachloride solution with the concentration of 40% for later use.

Second, method

Randomly dividing SPF SD rats into 15 groups, namely a normal control group and a model control group (CCl)₄Treated group), positive control group (300mg/kg bw Silymarin (Silymarin) -treated group), decoction of branches and leaves of photorhaponticum, and lightThe preparation method comprises the following steps of soaking the branches and leaves of the leafflower in hot water, soaking the branches and leaves of the leafflower in normal temperature, soaking the branches and leaves of the leafflower in the bright leaf, freeze-dried and crushed branches and leaves of the leafflower, leafflower branch and leaf paste, leafflower bud or flower boiling liquid, leafflower bud or flower hot water soaking liquid, leafflower bud or flower normal temperature soaking liquid, leafflower bud or flower tea, leafflower bud or flower freeze-dried and crushed substances and leafflower bud or flower paste, and each group contains 12 leaves.

After the SD rat is acclimatized for one week, the SD rat is injected with 40% CCl twice per week at fixed time according to the amount of 0.1mL/100g body weight₄Continuous injection for 8 weeks;

intraperitoneal injection of CCl₄Starting on the first day of (1), animals of each group were gavaged at the designed concentration for each group, placebo and CCl₄The treatment groups were perfused with pure water of the same volume, and the other groups were separately perfused with the corresponding drug solutions for 1/day for 8 weeks, mice were sacrificed, and livers were collected.

Liver tissues of each group of SD rats are collected and paraffin sections are made, and H & E staining is carried out to observe pathological damage conditions of the liver tissues. Paraffin sections are dewaxed and stained by sirius red stain, and after cell nuclei are lightly stained by hematoxylin, the distribution condition of collagen in liver tissues is observed microscopically.

The kit method is used for detecting the contents of Malondialdehyde (MDA), superoxide dismutase (SOD) and Glutathione (GSH) in the liver, and the kit method is used for detecting the contents of ALT and AST in serum according to the SD rat treated in the example 2, the blood of each group of SD rat is collected, after natural coagulation is carried out for 20min at room temperature, centrifugation is carried out for 10min at 1000rpm, the upper serum is collected, and the contents of ALT and AST in the serum are respectively detected. Collecting livers of each group of experimental animals, grinding the livers to prepare homogenate, and detecting the contents of MDA, SOD and GSH in liver tissues.

Collecting blood of each group of experimental animals, naturally coagulating at room temperature for 20min, centrifuging at 1000rpm for 10min, collecting upper serum, and detecting the expression levels of TNF-alpha, TGF-beta 1 and IL-17 in each group of mouse serum by ELISA method.

Three, result in

1. The invention has the effect on the liver index of rats

As can be seen from Table 8, CCl was compared with that of the normal control group₄The liver index of the rats in the treatment group is obviously increased, and the difference has statistical significance (P is less than 0.01), so that the modeling method can successfully cause the liver function damage of the rats.

TABLE 8 liver index in rats

Compared with a model control group, the rat liver indexes of the glabrous leaf branch and leaf boiling liquid, the glabrous leaf branch and leaf hot water soaking liquid, the glabrous leaf branch and leaf normal-temperature soaking liquid, the glabrous leaf branch and leaf hot water soaking liquid, the glabrous leaf branch and leaf freeze-dried crushed substance and the glabrous leaf branch and leaf sauce group are remarkably reduced, and the differences have statistical significance (P is less than 0.05), so that the glabrous leaf branch and leaf boiling liquid, the glabrous leaf branch and leaf hot water soaking liquid, the glabrous leaf branch and leaf normal-temperature soaking liquid, the glabrous leaf branch and leaf tea, the glabrous leaf branch and leaf freeze-dried crushed substance and the glabrous leaf branch and leaf sauce group can remarkably inhibit liver function damage caused by carbon tetrachloride. Compared with a model control group, the liver index of the mouse of the positive control group is obviously reduced, and the difference has statistical significance (P is less than 0.05), so that the positive control can obviously inhibit the liver function damage caused by carbon tetrachloride. The rat liver indexes of the glabrous leaf bud bract and flower boiling liquid, the glabrous leaf bud bract and flower hot water soaking liquid, the glabrous leaf bud bract and flower normal-temperature soaking liquid, the glabrous leaf bud bract and flower tea, the glabrous leaf bud bract and flower freeze-dried crushed matter and the glabrous leaf bud bract and flower paste group are not obviously reduced, the difference has no statistical significance (P is more than 0.05), and the liver functions of the glabrous leaf bud bract and flower boiling liquid, the glabrous leaf bud bract and flower hot water soaking liquid, the glabrous leaf bud bract and flower normal-temperature soaking liquid, the glabrous leaf bud bract and flower tea, the glabrous leaf bud bract and flower freeze-dried crushed matter and the glabrous leaf bud bract and flower paste can not obviously inhibit the damage caused by carbon tetrachloride.

2. Effect of the invention on the Activity of AST and ALT in rat serum

As can be seen from Table 9, compared with the normal control group, the AST and ALT activities of the rat serum of the model control group are significantly increased, and the difference has statistical significance (P is less than 0.01), which indicates that the modeling method can successfully cause the liver function damage of the rat.

TABLE 9 AST and ALT Activity in rat serum

Compared with a model control group, the rat serum AST and ALT activities of the glabrous leafflower branch and leaf boiling liquid, the glabrous leafflower branch and leaf hot water soaking liquid, the glabrous leafflower branch and leaf normal-temperature soaking liquid, the glabrous leafflower branch and leaf tea, the glabrous leafflower branch and leaf freeze-dried crushed substance and the glabrous leafflower branch and leaf paste group are remarkably reduced, and the differences have statistical significance (P is less than 0.01), so that the glabrous leafflower branch and leaf boiling liquid, the glabrous leafflower branch and leaf hot water soaking liquid, the glabrous leafflower branch and leaf normal-temperature soaking liquid, the glabrous leafflower branch and leaf tea, the glabrous leafflower branch and leaf freeze-dried crushed substance and the glabrous leafflower branch and leaf paste can remarkably inhibit the liver function damage caused by carbon tetrachloride. Compared with a model control group, the activities of AST and ALT in the serum of the rat of the positive control group are obviously reduced, and the difference has statistical significance (P is less than 0.01), which indicates that the positive control of the invention can obviously inhibit the liver function damage caused by carbon tetrachloride. Compared with a model control group, the liver function damage caused by carbon tetrachloride can not be obviously inhibited by the glabrous leaf bud and flower boiling liquid, the glabrous leaf bud and flower hot water soaking liquid, the glabrous leaf bud and flower normal-temperature soaking liquid, the glabrous leaf bud and flower tea, the glabrous leaf bud and flower freeze-dried crushed substances, the rat serum AST and ALT activity of the glabrous leaf bud and flower paste group are not obviously reduced, the difference has no statistical significance (P is more than 0.05), and the liver function damage caused by carbon tetrachloride can not be obviously inhibited by the glabrous leaf bud and flower boiling liquid, the glabrous leaf bud and flower hot water soaking liquid, the glabrous leaf bud and flower normal-temperature soaking liquid, the glabrous leaf bud and flower tea, the glabrous leaf bud and flower crushed substances, and the glabrous leaf bud and flower paste.

3. The Effect of the invention on the expression levels of MDA, SOD and GSH in rat serum

As shown in Table 10, compared with the normal control group, the expression level of MDA in the serum of the rat of the model control group is obviously increased, the expression levels of SOD and GSH are obviously reduced, and the difference has statistical significance (P is less than 0.01) or (P is less than 0.05). Description of CCl₄Can induce rat to produce inflammation to result in lipid peroxidation of body and damage to body.

TABLE 10 Activity of SOD, MDA, GSH in rat liver tissue

Note: p <0.01 compared to normal control group; compared with the model control group, # p <0.05, # p < 0.01.

And CCl₄Compared with treatment groups, the serum of the photoleaf branch and leaf boiling liquid, the photoleaf branch and leaf hot water soaking liquid, the photoleaf branch and leaf normal-temperature soaking liquid, the photoleaf branch and leaf tea, the photoleaf branch and leaf freeze-dried crushed substance and the photoleaf branch and leaf sauce group of the invention has obviously reduced MDA level, obviously increased SOD and GSH level and statistical significance (P is less than 0.01) of difference. And other groups with CCl₄The treatment group showed no significant difference (P > 0.05). The MDA content reflects the lipid peroxidation degree of the organism and the damage degree of the organism; SOD is an important enzyme for eliminating active oxygen in organisms and is the first defense line of a biological antioxidant system; GSH can remove free radicals, and can block the secondary reaction of free radicals caused by lipid peroxidation, thereby reducing the damage of lipid peroxidation to organisms and protecting cell membranes from being damaged by peroxidation. Thus, this example demonstrates that photophylla branch and leaf boiling solution, photophylla branch and leaf hot water infusion solution, photophylla branch and leaf normal temperature infusion solution, photophylla branch and leaf tea, photophylla branch and leaf freeze-dried powderThe crushed material, the Guangdong leaf flower branch and leaf sauce can reduce CCl by reducing the levels of MDA, SOD and GSH₄Free radical and lipid peroxidation induced after treatment can prevent liver injury.

Fourthly, summarize

In this example, the branches and leaves of the photoleaf flowers of the present invention significantly inhibited carbon tetrachloride-induced liver function damage, and the present invention is superior to photoleaf flowers and bracts, and flowers in inhibiting carbon tetrachloride-induced liver function damage. The cotyledon ladysthum branches and leaves can obviously reduce the liver index of rats with liver injury caused by carbon tetrachloride and ALT and AST activity in serum, reduce MDA level in liver tissues, improve GSH and SOD level in liver tissues and reduce liver injury caused by lipidosome peroxidation damage.

Example 5

Acute toxicity test of the present invention

Taking 50 male and female mice with the weight of 18-25 g of SPF-grade normal KM mice respectively, randomly dividing the male and female mice into 5 groups according to sex and weight, wherein each group comprises 10 mice, 3600, 3240, 2916, 2624 and 2361mg crude drugs/kg of the ramulus et folium Gaultheriae Yunnanensis freeze-dried powder in the gavage example 1 are respectively filled in the stomach, the dose distance between two adjacent groups is 0.9, the gavage volume is 0.1ml/10g of the weight, the toxic reaction, death distribution and the number of dead animals of the mice in one week after administration are observed, and LD50 (half lethal dose) and 95 percent confidence limit thereof are calculated according to a Bliss method. The result shows that the mice have reduced autonomic activity, calm and lie, slowed respiration and behavior disorder caused by oral relay after about 2min after the high-dose gavage, and die after about 10-30 min. The animals which do not die gradually recover normal respiration after 40min, the behaviors and the like recover to normal after 1h, and the animals do not die within 7 days later. The dead mice have no abnormality in the heart, lung, liver and other major organs at the naked eye autopsy. The LD50 of the female mouse is 2425.72 (2354.31-2584.39) mg of crude drug/kg; the male mouse LD50 is 2543.56 (2414.38-2691.37) mg crude drug/kg, and the male and female animals LD50 are similar and have no obvious difference.

Example 6

Tablet preparation

Respectively taking 40g of the freeze-dried powder of the branches and leaves of the glabrous leaf in the embodiment 1 of the invention, mixing the freeze-dried powder with 210g of starch and 9g of dextrin uniformly, adding 12% starch slurry to prepare a soft material, granulating by using a nylon screen with 10 meshes, carrying out ventilation drying at 65 +/-1 ℃, grading by using a 12-mesh sieve, adding 3.0g of magnesium stearate and 11g of sodium carboxymethylcellulose, mixing uniformly, pressing into 2000 tablets, and coating to obtain the composition, wherein each tablet contains 25 mg. The dosage and frequency of administration will depend on clinical effectiveness.

Example 7

Capsule preparation

40g of the fig branches and leaves freeze-dried powder in the embodiment 1 of the invention is respectively taken, evenly mixed with 250g of starch and 5g of magnesium stearate, directly filled into 2000 granules by a full-automatic capsule filling machine, and polished to obtain the fig branches and leaves freeze-dried powder containing 25mg of each granule. The dosage and frequency of administration will depend on clinical effectiveness.

Example 8

Preparation of dropping pills

Taking 23g of the freeze-dried powder of the branches and leaves of the lespedeza glabra in the embodiment 1 of the invention, putting 65g of the freeze-dried powder into polyethylene glycol which is heated and melted, stirring until the mixture is dissolved, transferring the mixture into a liquid storage bottle, sealing and keeping the temperature at 75 +/-3 ℃, adjusting a liquid drop quantitative valve of a pill dropping machine, dropping the mixture into liquid paraffin with the temperature of 15-20 ℃ from top to bottom to prepare 2000 pills, draining and wiping off the liquid paraffin to obtain the dripping pills, wherein each pill contains 14 mg. The dosage and frequency of administration will depend on clinical effectiveness.

Example 9

Preparation of oral liquid

Respectively taking 30g of the freeze-dried powder of the branches and leaves of the fig. 1, mixing the freeze-dried powder with 500g of honey, 160g of cane sugar, 12g of sodium benzoate and 3000ml of distilled water, heating to 90 ℃, stirring to dissolve, preserving heat for 40min, filtering, adding water into the filtrate to dilute the filtrate to 14000ml, encapsulating (12 ml per branch), and sterilizing to obtain the fig.

Example 10

Preparation of granules

Respectively taking 6g of the fig. 1 lyophilized powder, 24g of dextrin, 450g of sucrose powder and a proper amount of ethanol, mixing, sieving with a 12-mesh sieve to prepare granules, drying the granules at 65 ℃, grading and subpackaging to obtain the fig. 2.0g of the weight of each bag.

Example 11

Preparation of injection

Respectively taking 40g of the fig. 1 freeze-dried powder, adding a proper amount of water for injection to dissolve, adding 0.02% of activated carbon in the preparation amount, stirring for 10-20 minutes, filtering, diluting the filtrate to about 8L, adding sodium chloride to adjust osmotic pressure to be isotonic, adjusting pH to 7.7-8.1, filtering, encapsulating into 800 pieces (10 ml/piece), and sterilizing to obtain the fig. 1 freeze-dried powder. Can be used for intravenous injection. The dosage and frequency of administration will depend on clinical effectiveness.

Example 12

Preparation of compound capsule

Respectively taking 25g of the fig. 1 freeze-dried powder, mixing with 25g of notoginsenoside, 185g of starch and a proper amount of ethanol, sieving with a 12-mesh sieve, drying, granulating, adding 2.5g of magnesium stearate, mixing, filling into 1000 granules by using a full-automatic capsule filling machine, and polishing to obtain the finished product. Each granule contains 25mg of notoginsenoside. Can be orally administered for preventing and treating various ischemic cardiovascular and cerebrovascular diseases. The dosage and frequency of administration will depend on clinical effectiveness.

Example 13

Food preparation of lyophilized powder of glabrous leafflower

10g of dry yeast, 180ml of warm water, a little water, 300g of flour, 10mg of the photoleaf flower, branch and leaf freeze-dried powder in the embodiment 1, 20g of vegetable oil and a little low-sodium salt;

the biscuit making method comprises the following steps: yeast is sprinkled in warm water, stirred and melted, and 100g of the lyophilized powder of branches and leaves of Calophyllum Inophyllum in example 1 is added. Adding flour, stirring, adding vegetable oil, and kneading into smooth dough; making the dough into thin slices with the thickness of 0.3 cm; pressing to form, pricking hole, spraying water on surface, spraying a little low sodium salt, and fermenting at room temperature for 15 min; preheating in oven at 130 deg.C, placing on the upper layer, and baking for about 12min to obtain food containing lyophilized extract of flos Phyllanthi.

Example 14

Preparation of health-care instant powder

300g of the freeze-dried powder of the fig branches and leaves in the example 1 is taken respectively to be mixed with a proper amount of auxiliary agents such as fruit juice powder, sugar and the like, the mixture is sieved by a 10-mesh sieve, dried at 65 ℃, and packaged into bags to prepare various medicinal granules with the functions of relieving alcoholism, protecting liver, reducing blood sugar and blood fat, wherein each bag contains 150mg of the freeze-dried powder of the fig branches and leaves in the example 1. The dosage and frequency of administration will depend on clinical effectiveness.

Example 15

Preparation of tea products

5g of the freeze-dried powder of the branches and leaves of the glabrous leafflower in the embodiment 1 is taken, 63kg of compound sweetening agent is added, the mixture is uniformly mixed and then is filled into a special paper bag, an aluminum-plastic compound bag is sleeved outside the paper bag, and the bag is sealed, wherein each bag is 6-10 g, so that the bagged glabrous leafflower health-care tea with the effects of dispelling the effects of alcohol, protecting the liver, reducing the blood sugar and reducing the blood fat is prepared. The dosage and frequency of administration will depend on clinical effectiveness.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. Application of glabrous leafflower in preparation of composition with liver protecting effect and/or anti-alcoholism effect is disclosed, wherein the application part of glabrous leafflower is flower and/or bract and/or branch and/or leaf of glabrous leafflower.

2. Use of flowers and/or bracts and/or branches and/or leaves of photoleaf flowers for preparing a composition having an effect of inhibiting elevation of glutamic-pyruvic transaminase and/or glutamic-oxalacetic transaminase.

3. Use of flowers and/or bracts and/or branches and/or leaves of photoleaf flowers for the preparation of a composition having an effect of increasing the activity of alcohol dehydrogenase and/or acetaldehyde dehydrogenase.

4. The application part of the photophyllanthus is the flower and/or bract and/or branch and/or leaf of the photophyllanthus.

5. Use according to claim 1 or 2 or 3 or 4, characterized in that: the composition comprises a pharmaceutical composition, a food composition, a health product composition, a tea product composition or a product containing the photorhaponticum uniflorum.

6. Use according to claim 5, characterized in that: the composition comprises flowers and/or bracts and/or branches and/or leaves of the photoleaf flowers and an acceptable carrier, wherein the flowers and/or bracts and/or branches and/or leaves of the photoleaf flowers are active ingredients, and the active ingredients account for 1-99% of the composition by weight.

7. Use according to claim 1 or 2 or 3 or 4 or 5 or 6, characterized in that: boiling flowers and/or bracts and/or branches and/or leaves of the glabrous leafflower with water to obtain boiling liquid for application, or soaking the flowers and/or bracts and/or branches and/or leaves with water to obtain soaking liquid for application.

8. Use according to claim 1 or 2 or 3 or 4 or 5 or 6, characterized in that: lyophilizing flower and/or bract and/or branch and/or leaf of Calophyllum Inophyllum L to obtain powder for use and/or direct use.

9. Use of photophyllanthus according to claim 8, characterized in that: withering the flowers and/or bracts and/or branches and/or leaves before boiling in water or soaking or freeze-drying into powder.

10. A composition with the effects of relieving alcoholism and/or protecting liver is characterized in that: the effective component is flower and/or bract and/or branch and/or leaf of photoleaf flower.

11. The composition according to claim 10, wherein the composition has an anti-hangover effect and/or a liver-protecting effect, and comprises: the application form of the flowers and/or bracts and/or branches and/or leaves of the glabrous leafflower is to prepare tablets, dripping pills, capsules, granules or oral liquid with auxiliary materials.