CN111808734A - Bacterial colony sampling device for biotechnology - Google Patents
Bacterial colony sampling device for biotechnology Download PDFInfo
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- CN111808734A CN111808734A CN202010629031.9A CN202010629031A CN111808734A CN 111808734 A CN111808734 A CN 111808734A CN 202010629031 A CN202010629031 A CN 202010629031A CN 111808734 A CN111808734 A CN 111808734A
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- sample
- culture dish
- injector
- fixedly connected
- pipe
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- 238000005070 sampling Methods 0.000 title claims abstract description 49
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 29
- 238000012360 testing method Methods 0.000 claims abstract description 21
- 238000010079 rubber tapping Methods 0.000 claims abstract description 11
- 238000007599 discharging Methods 0.000 claims description 5
- 238000009434 installation Methods 0.000 claims 7
- 238000006073 displacement reaction Methods 0.000 abstract description 3
- 238000003780 insertion Methods 0.000 description 9
- 230000037431 insertion Effects 0.000 description 9
- 239000012895 dilution Substances 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a bacterial colony sampling device for biotechnology, which comprises a fixed seat, an injector and test tubes, wherein a culture dish is arranged inside a splicing block, a pipe sleeve is fixedly connected to the top of the fixed seat, telescopic pipes are movably connected to the outer sides of the pipe sleeves, one sides of the two telescopic pipes, which are far away from the culture dish, are fixedly connected with a first mounting plate, the injector is arranged between the two liquid storage bottles, the bottom of the injector is fixedly communicated with a main port at the top of a tapping device, one sides of the two telescopic pipes, which are directly close to the culture dish, are fixedly connected with a second mounting plate, and a plurality of test tubes are arranged in the: connect the tapping ware through the syringe and can carry out the sample of many places simultaneously, can carry out the layering sample and dilute the sample through the liquid reserve tank tapping, thereby the slipmat can prevent when taking a sample mistake touch the device and cause the device displacement to influence the sample, adjust the telescopic link through the plug gim peg in order to control the sample height, install different sample equipment.
Description
Technical Field
The invention relates to a sampling device, in particular to a bacterial colony sampling device for biotechnology, and belongs to the field of biological sampling devices.
Background
Along with the development of biotechnology, the popularization of biological products, biotechnology has become more and more important, and the research of taking a sample of bacterial colony is required to carry out in the production of biotechnology, takes a sample mainly to divide into random sampling and nonrandom sample from statistics perspective, and random sampling includes many sampling methods such as equidistance sample, stratified sampling, dilution sample, will use sampling device when taking a sample the research.
Present bacterial colony sampling device for biotechnology, it is bulky, the operation is complicated, can't carry out non-random sampling, and can't carry out many places samples simultaneously when the sample, not only reduced sampling efficiency and quality, still improved intensity of labour.
Disclosure of Invention
The invention provides a bacterial colony sampling device for biotechnology, which is used for solving the problems.
In order to solve the technical problems, the invention provides the following technical scheme:
the invention relates to a bacterial colony sampling device for biotechnology, which comprises a fixed seat, an injector and a test tube, wherein an insertion block is fixedly connected at the inner end of the fixed seat, a culture dish is arranged at the inner side of the insertion block, an insertion plate is fixedly connected at the bottom end of the culture dish, a tube sleeve is fixedly connected at the top of the fixed seat, a telescopic tube is movably connected at the outer side of the tube sleeve, a fixed bolt is arranged at the outer side of the tube sleeve, a first mounting plate is fixedly connected at one side of the two telescopic tubes away from the culture dish, two liquid storage bottles are correspondingly arranged at two sides of the first mounting plate close to the telescopic tubes, a liquid discharge port is arranged at the bottom of each liquid storage bottle, a liquid discharge pipe is fixedly communicated with the bottom of the liquid discharge port and extends into the culture dish, the injector is, the fixed intercommunication of main mouthful of syringe bottom and tapping ware top, the syringe openly is provided with a plurality of minutes mouthful, two flexible pipe is direct to be close to culture dish one side fixedly connected with mounting panel two, be provided with a plurality of test tubes in the mounting panel two, the test tube passes through two swing joint of mount pad and mounting panel, the test tube top is through the takeover and the fixed intercommunication of branch mouthful, the test tube openly is provided with the sample connection, the fixed intercommunication of sample connection one end and sampling tube one end, the sampling tube other end and the fixed intercommunication of sampling needle, the sampling needle extends to in the culture dish.
As a preferred technical scheme of the invention, the bottom of the fixed seat is fixedly connected with an anti-skid pad.
As a preferred technical scheme of the invention, two ends of the insertion plate are movably connected with the insertion block.
As a preferable technical scheme of the invention, the fixing bolt penetrates through the telescopic pipe and the pipe sleeve.
As a preferred technical scheme of the invention, the telescopic pipe is provided with four hooks.
As a preferable technical scheme of the invention, the liquid discharging port is provided with a valve.
The invention has the following beneficial effects: connect the sample that the tapping ware can carry out many places simultaneously in the culture dish through the syringe, put the liquid through the liquid reserve tank and can carry out the layering sample and dilute the sample, be favorable to promoting the efficiency and the precision of sample.
The couple can be accomodate bleeder line, sampling tube and decomposition tube, thereby the slipmat can prevent when taking a sample mistake and touch the device and cause the device displacement to influence the sample, adjusts the telescopic link through the plug gim peg in order to control the sample height, installs different sampling equipment.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of a tap of the present invention;
FIG. 3 is a schematic view of the structure of the culture dish of the present invention.
In the figure: 1. a fixed seat; 2. a non-slip mat; 3. an insertion block; 4. a plugboard; 5. a culture dish; 6. pipe sleeve; 7. a telescopic pipe; 8. a fixing bolt; 9. hooking; 10. a first mounting plate; 11. a second mounting plate; 12. a liquid storage bottle; 13. a tapping port; 14. a valve; 15. a liquid discharging pipe; 16. an injector; 17. a mounting seat; 18. a tap; 19. a main port; 20. opening; 21. a connection pipe is connected; 22. a test tube; 23. a sampling port; 24. a sampling tube; 25. a sampling needle.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example (b): as shown in fig. 1-3, a bacterial colony sampling device for biotechnology comprises a fixed base 1, an injector 16 and a test tube 22, wherein an insertion block 3 is fixedly connected to the inner end of the fixed base 1, a culture dish 5 is arranged on the inner side of the insertion block 3, an insertion plate 4 is fixedly connected to the bottom end of the culture dish 5, a pipe sleeve 6 is fixedly connected to the top of the fixed base 1, a telescopic pipe 7 is movably connected to the outer side of the pipe sleeve 6, a fixed bolt 8 is arranged on the outer side of the pipe sleeve 6, a mounting plate I10 is fixedly connected to one side of the two telescopic pipes 7 away from the culture dish 5, two liquid storage bottles 12 are correspondingly arranged on two sides of the mounting plate I10 close to the telescopic pipe 7, the liquid storage bottles 12 can store layered liquid and dilution liquid, a liquid discharge port 13 is arranged at the bottom of each liquid storage bottle 12, a liquid discharge pipe 15 is fixedly communicated with the, syringe 16 is through mount pad 17 and 10 swing joint of mounting panel, the fixed intercommunication of the main mouth 19 at 16 bottoms of syringe and the 18 tops of tapping device, syringe 16 openly is provided with a plurality of taps 20, two flexible pipes 7 are direct to be close to 5 one side fixedly connected with mounting panel two 11 of culture dish, be provided with a plurality of test tubes 22 in the mounting panel two 11, a plurality of test tubes 22 are convenient for deposit many sample samples, test tube 22 is through mount pad 17 and two 11 swing joints of mounting panel, test tube 22 top is through the fixed intercommunication of tapping pipe 21 with tap 20, test tube 22 openly is provided with sample connection 23, sample connection 23 one end and the fixed intercommunication of 24 one ends of sampling tube, the 24 other ends of sampling tube and the fixed intercommunication of sampling needle 25, sampling needle 25 extends to in the culture dish 5, conveniently carry out many places sample.
Fixed base 1 bottom fixedly connected with slipmat 2, the device takes place the displacement influence sample result by mistake when slipmat 2 can prevent to take a sample, 4 both ends of plugboard and 3 swing joint of grafting piece, and the gim peg 8 runs through flexible pipe 7 and pipe box 6, is provided with four couples 9 on the flexible pipe 7, and couple 9 can be accomodate the body, is provided with valve 14 on the drain 13.
Specifically, when the invention is used, a culture dish 5 required to be sampled is movably fixed in an inserting block 3 through an inserting plate 4, a fixing bolt 8 is pulled out, a telescopic pipe 7 is moved to adjust the sampling height and then is inserted into the fixing bolt 8 to fix the position, an injector 16 is pulled to draw air in a test tube 22 through a tap-off device 18, bacterial colonies in the culture dish 5 are sucked under the action of atmospheric pressure through a sampling needle 25 and enter the test tube 22 through a sampling tube 24, a valve 14 can be opened when layered sampling and dilution sampling are required, layered liquid and diluent in a liquid storage bottle 12 enter the culture dish 5 from a liquid discharge port 13 through a liquid discharge pipe 15, sampling is carried out in multiple modes, the sampling efficiency and the sampling quality are favorably improved, if the device is touched by mistake during sampling, the device can be prevented from being displaced due to the reason of higher friction force of an antiskid pad 2, and when the sampling is finished, a tube body can be stored and stored through a, is beneficial to improving the stability and the functionality of the device.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the equipment or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The bacterial colony sampling device for the biotechnology is characterized by comprising a fixed seat (1), an injector (16) and a test tube (22), wherein an inserting block (3) is fixedly connected to the inner end of the fixed seat (1), a culture dish (5) is arranged on the inner side of the inserting block (3), an inserting plate (4) is fixedly connected to the bottom end of the culture dish (5), a pipe sleeve (6) is fixedly connected to the top of the fixed seat (1), a telescopic pipe (7) is movably connected to the outer side of the pipe sleeve (6), a fixing bolt (8) is arranged on the outer side of the pipe sleeve (6), a first mounting plate (10) is fixedly connected to one side, away from the culture dish (5), of the two telescopic pipes (7), two liquid storage bottles (12) are correspondingly arranged on two sides, close to the telescopic pipe (7), liquid discharging ports (13) are arranged at the bottoms of the liquid storage bottles (12), and liquid discharging pipes (15), the liquid discharging pipe (15) extends into the culture dish (5), two an injector (16) is arranged between the liquid storage bottles (12), an installation seat (17) is arranged on the injector (16), the injector (16) is movably connected with a first installation plate (10) through the installation seat (17), the bottom of the injector (16) is fixedly communicated with a main port (19) at the top of a tapping device (18), the injector (16) is positively provided with a plurality of taps (20), two telescopic pipes (7) are directly close to a second installation plate (11) fixedly connected with one side of the culture dish (5), the second installation plate (11) is internally provided with a plurality of test tubes (22), the test tubes (22) are movably connected with the second installation plate (11) through the installation seat (17), the top of the test tubes (22) is fixedly communicated with the taps (20) through a tapping pipe (21), and the test tubes (22) are positively provided with sampling ports (23), sample connection (23) one end and sampling tube (24) one end fixed intercommunication, sampling tube (24) other end and sample needle (25) fixed intercommunication, sample needle (25) extend to in culture dish (5).
2. The colony sampling device for biotechnology according to claim 1, characterized in that the bottom of fixing base (1) is fixedly connected with non-slip mat (2).
3. A colony sampling device for biotechnology according to claim 1, characterized in that both ends of said plug board (4) are movably connected with the plug block (3).
4. A colony sampling device for biotechnology according to claim 1, characterized in that the dead bolt (8) runs through the telescopic tube (7) and the pipe sleeve (6).
5. A colony sampling device for biotechnology according to claim 1, characterized in that said telescopic pipe (7) is provided with four hooks (9).
6. A colony sampling device for biotechnology as claimed in claim 1, wherein the tapping (13) is provided with a valve (14).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202010629031.9A CN111808734A (en) | 2020-07-01 | 2020-07-01 | Bacterial colony sampling device for biotechnology |
Applications Claiming Priority (1)
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CN202010629031.9A CN111808734A (en) | 2020-07-01 | 2020-07-01 | Bacterial colony sampling device for biotechnology |
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CN111808734A true CN111808734A (en) | 2020-10-23 |
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CN202010629031.9A Pending CN111808734A (en) | 2020-07-01 | 2020-07-01 | Bacterial colony sampling device for biotechnology |
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Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1471865A (en) * | 1973-04-06 | 1977-04-27 | Unilever Ltd | Dispensers for use in bacteriology and similar biochemical analysis |
FR2525770A1 (en) * | 1982-04-23 | 1983-10-28 | Issa Georges | Multi:cylinder laboratory syringe for biology, bacteriology titrations - cultures, etc., contaminates only nozzle head which is removable for sterilising |
US5871699A (en) * | 1995-01-10 | 1999-02-16 | Ruggeri; Guido | Apparatus and method for drawing liquid samples and dispensing them into a plurality of test tubes |
JP2012213333A (en) * | 2011-03-31 | 2012-11-08 | Rabotekku Kk | Sample making device |
CN107083324A (en) * | 2017-04-14 | 2017-08-22 | 云南省食品药品监督检验研究院 | A kind of portable quick collection fungus strain system examined soon for filling drinking water microorganism |
CN208803074U (en) * | 2018-11-30 | 2019-04-30 | 天津市疾病预防控制中心 | A kind of compound microbial sampler |
CN110354932A (en) * | 2019-08-06 | 2019-10-22 | 广西曼森生物科技有限公司 | A kind of automatic fluid operating platform |
WO2020008067A1 (en) * | 2018-07-06 | 2020-01-09 | Ge Healthcare Bio-Sciences Corp. | Sampling device and method |
CN110804644A (en) * | 2019-11-22 | 2020-02-18 | 阮雁春 | Method and device for detecting multiple microorganisms in food |
CN210127238U (en) * | 2019-03-12 | 2020-03-06 | 广东出入境检验检疫局检验检疫技术中心 | Food microorganism detection device |
-
2020
- 2020-07-01 CN CN202010629031.9A patent/CN111808734A/en active Pending
Patent Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1471865A (en) * | 1973-04-06 | 1977-04-27 | Unilever Ltd | Dispensers for use in bacteriology and similar biochemical analysis |
FR2525770A1 (en) * | 1982-04-23 | 1983-10-28 | Issa Georges | Multi:cylinder laboratory syringe for biology, bacteriology titrations - cultures, etc., contaminates only nozzle head which is removable for sterilising |
US5871699A (en) * | 1995-01-10 | 1999-02-16 | Ruggeri; Guido | Apparatus and method for drawing liquid samples and dispensing them into a plurality of test tubes |
JP2012213333A (en) * | 2011-03-31 | 2012-11-08 | Rabotekku Kk | Sample making device |
CN107083324A (en) * | 2017-04-14 | 2017-08-22 | 云南省食品药品监督检验研究院 | A kind of portable quick collection fungus strain system examined soon for filling drinking water microorganism |
WO2020008067A1 (en) * | 2018-07-06 | 2020-01-09 | Ge Healthcare Bio-Sciences Corp. | Sampling device and method |
CN208803074U (en) * | 2018-11-30 | 2019-04-30 | 天津市疾病预防控制中心 | A kind of compound microbial sampler |
CN210127238U (en) * | 2019-03-12 | 2020-03-06 | 广东出入境检验检疫局检验检疫技术中心 | Food microorganism detection device |
CN110354932A (en) * | 2019-08-06 | 2019-10-22 | 广西曼森生物科技有限公司 | A kind of automatic fluid operating platform |
CN110804644A (en) * | 2019-11-22 | 2020-02-18 | 阮雁春 | Method and device for detecting multiple microorganisms in food |
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Application publication date: 20201023 |