CN107083324A - A kind of portable quick collection fungus strain system examined soon for filling drinking water microorganism - Google Patents
A kind of portable quick collection fungus strain system examined soon for filling drinking water microorganism Download PDFInfo
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- CN107083324A CN107083324A CN201710244026.4A CN201710244026A CN107083324A CN 107083324 A CN107083324 A CN 107083324A CN 201710244026 A CN201710244026 A CN 201710244026A CN 107083324 A CN107083324 A CN 107083324A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
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Abstract
The invention discloses a kind of portable quick collection fungus strain system examined soon for filling drinking water microorganism, including control box, chamber door, five collection bacterium devices, uviol lamp, power supply boxs.The present invention is easy to carry, and directly can be carried out in situ sampling, collection bacterium in water factory to be checked, not only with not limited by use environment condition, and can effectively shorten detection cycle；The present invention is enclosed construction, can effectively prevent from being contaminated, the accuracy of effective guarantee collection bacterium process；The present invention can be cultivated by different bacterium to be needed, and water sample is evacuated into the first bacteria gathering device, in the second bacteria gathering device, and solving needs the shortcoming of multiple sampling during traditional detection, is easy to the later stage to different strain classifted training；The present invention can extract filling water sample, with easy to operate, collect bacterium efficiency high, substantially reduce the advantage of hand labor intensity；The present invention outside can also carry often use detection device, have the advantages that using scope it is wide.
Description
Technical field
The invention belongs to Microbiological detection of foods technical field, and in particular to one kind is examined soon for filling drinking water microorganism
Portable quick collection fungus strain system.
Background technology
At present, fill water increasingly becomes the main drinking water source of common people, and market demand constantly increases, manufacturer
It is on the increase, but water bottle used in the quality problems of incident fill water, especially fill water, bucket is safe and healthy
Problem turns into the focus of social concerns.Food-borne biological pollution is one of key factor of filling water pollution, mainly including bacterium colony
The pathogenic bacteria such as sum, coliform, mould yeast, pseudomonas aeruginosa.According to document and, the bottled water of multiple provinces and cities is all deposited
In a large amount of underproof phenomenons, fill water quality testing and the monitoring big Yushan Hill of responsibility.
Causing the factor of filling water pollution has several aspects, and such as water source is contaminated, or sanitary control is not in production process
Strictly, the non-sterile hand of practitioner is directly contacted with mineral water or container inner wall, or packing container does not carry out thoroughly cleaning
The factors such as sterilization all can cause the microorganism in fill water exceeded.
It is to need staff to go to water factory to be checked to sample first, by sample fill water generally during fill water sampling observation
Bucket transports go back to laboratory and is collecting bacteria, cultivates, detects, whole detection cycle is usually required 15 ~ 20 days, and detection cycle is longer.Though
So occur in that some new microorganisms examine means soon at present, but it is primarily present and limited by use environment condition,
Related work can only be completed in laboratory.Generally when being collecting bacteria after fill water reaches laboratory, for mould yeast, verdigris
Pseudomonad is collecting bacteria using membrane-filter procedure as defined in standard, and is culture of directly drawing water for coliform
, for tradition sampling observation equipment, there is needs the shortcoming of repeated multiple times sampling, and sampling efficiency is very low, especially for normal
The 18.9L seen bottled water, generally sampling observation object is every group of 5 barrels of bottled waters, at this time needs that water is manually poured on difference respectively
Filtering collection bacterium in filter membrane, operation is not only inconvenient, and labor intensity is big；And current sampling detecting instrument is all open
, also in the presence of easy contaminated problem, and then cause the result of the test of generation false positive.Also, traditional sampling observation equipment is
It is every filtered a filter bowl after, be required for flame burn filter disc, while time-consuming also be not easy ensure experiment accuracy, in addition,
There is very big potential safety hazard with high temperature naked light in superclean bench or Biohazard Safety Equipment.
Therefore, research and development one kind is easy to carry, it can directly be collecting bacteria, be ensured while shortening detection cycle in sampling
The accuracy of sampling, prevents that water sample is contaminated during collection bacterium, while the collection fungus strain system for collecting bacterium efficiency high is to solve above-mentioned ask
The key of topic.
The content of the invention
It is an object of the invention to provide a kind of portable quick collection fungus strain system examined soon for filling drinking water microorganism.
The object of the present invention is achieved like this, including control box, chamber door, five collection bacterium devices, uviol lamp, power supply boxs,
Described chamber door is located at the side of control box, and the front of described control box is provided with the observation for being used for checking control box built-in function
Window, the front of described control box, which is provided with gathering hole, described gathering hole, is provided with gloves, the running-on edge of described gloves
It is connected with the edge seal of gathering hole, described collection bacterium device is arranged in control box, described collection bacterium device includes the first collection
Bacterium device, the second bacteria gathering device, suction hose, drawn water syringe needle, and suction hose is connected with the top of the first described bacteria gathering device, described
The water inlet end of suction hose connects the syringe needle that draws water, and the top that the second described bacteria gathering device passes through flexible pipe and suction hose is connected, institute
Top of the uviol lamp stated in control box, described power supply box is located at the outside of control box.
Beneficial effects of the present invention：The present invention is easy to carry, and directly can be carried out in situ sampling, collection bacterium in water factory to be checked,
It can not only be limited by use environment condition, and detection cycle can be effectively shortened；The present invention is enclosed construction,
It can effectively prevent from being contaminated, the accuracy of effective guarantee collection bacterium process；The present invention can be cultivated by different bacterium to be needed, by water sample
It is evacuated to the first bacteria gathering device, in the second bacteria gathering device, solving needs the shortcoming of multiple sampling during traditional detection, is easy to the later stage pair
Different strain classifted training；The present invention can extract filling water sample, with easy to operate, collect bacterium efficiency high, substantially reduce artificial
The advantage of labor intensity；The present invention outside can also carry often use detection device, have the advantages that using scope it is wide.
Brief description of the drawings
Fig. 1 is schematic structural view of the invention；
Fig. 2 is the single structural representation for collecting bacterium device；
Fig. 3 for collection bacterium device draw water sample when working state structure schematic diagram；
Fig. 4 is the overlooking the structure diagram of bucket seat；
Fig. 5 is the overlooking the structure diagram of rack：
In figure：1- control boxs, 2- chamber doors, 3a- sample buckets, 3b- barrels of seats, 3c- water draining beaks, 3d- collection bacterium filter membranes, 4a- syringes, 4b-
Piston push rod, 4c- faucets, 4d- water inlet taps, 4e- ventilation nozzles, 5- suction hoses, 6- draws water syringe needle, and 7- peristaltic pumps, 8- is ultraviolet
Lamp, 9- observation windows, 10- gathering holes, 11- tag boards, 12a- fixed wafers, 12b- rubber cufves, 12c- ventilation plugs, 13- is placed
Frame, 13a- supports, 13b- places plate, 13c- connecting upright poles, 13d- lassos, water tube hose under 14-, 15- the first carry parts, 16- the
Two carry parts, 17- protective caps, 18- handles, 19- blocks cap, 20- fill waters, 21- pipe clamps.
Embodiment
The present invention is further illustrated below in conjunction with the accompanying drawings, but the present invention is not any limitation as in any way, base
In present invention teach that any conversion or replacement made, belong to protection scope of the present invention.
The present invention as shown in 1 ~ Fig. 5 of accompanying drawing includes control box 1, chamber door 2, five collection bacterium devices, uviol lamp 8, power supply boxs, institute
The chamber door 2 stated is located at the side of control box 1, and the front of described control box 1 is provided with the sight for being used for checking the built-in function of control box 1
Examine window 9, the front of described control box 1, which is provided with gathering hole 10, described gathering hole 10, is provided with gloves, described gloves
Running-on edge is connected with the edge seal of gathering hole 10, and described collection bacterium device is arranged in control box 1, described collection bacterium device
Including the first bacteria gathering device, the second bacteria gathering device, suction hose 5, draw water syringe needle 6, is connected with and takes out at the top of the first described bacteria gathering device
Water tube hose 5, the water inlet end of described suction hose 5 connects the syringe needle 6 that draws water, and the second described bacteria gathering device is soft with drawing water by flexible pipe
The top connection of pipe 5, the top that described uviol lamp 8 is located in control box 1, described power supply box is located at the outside of control box 1.
The first described bacteria gathering device includes passing through spiral shell between sample bucket 3a, bucket seat 3b, described sample bucket 3a and bucket seat 3b
Line structure movement is connected, and described sample bucket 3a top is provided with water inlet, steam vent, and described bucket seat 3b bottom is indent
Structure, described bucket seat 3b bottom centre is provided with water draining beak 3c, described bucket seat 3b provided with collection bacterium filter membrane 3d, and described takes out
Water outlet one end of water tube hose 5 is connected with sample bucket 3a water inlet, and described water draining beak 3c is provided with blocking cap, described sample bucket
The bottom of 3a steam vent is provided with air filter film.
The second described bacteria gathering device includes syringe 4a, piston push rod 4b, and described syringe 4a bottom is provided with faucet 4c,
A described piston push rod 4b part is in the syringe 4a, and the side at described syringe 4a middle part is provided with water inlet tap 4d, institute
Syringe 4a relative the water inlet tap 4d that states opposite side passes through flexible pipe and suction hose provided with ventilation nozzle 4e, described water inlet tap 4d
5 top connection.
The body of the water inlet end of described suction hose 5 is arranged with closing device.
Described closing device includes fixed wafer 12a, rubber cuff 12b, and described fixed wafer 12a, which is sheathed on, to draw water
The body of the water inlet end of flexible pipe 5, described rubber cuff 12b and fixed wafer 12a edge is consolidated, described fixed wafer
12a is provided with ventilation plug 12c.
Described closing device includes fixed wafer 12a, adhesive sticker ring plate, and described fixed wafer 12a, which is sheathed on, to draw water
The body of the water inlet end of flexible pipe 5, described adhesive sticker ring plate is located on fixed wafer 12a bottom surface, described fixed wafer 12a
Provided with ventilation plug 12c.
It is provided with rack 13 in described control box 1, described the first bacteria gathering device, the second bacteria gathering device are located at rack 13
On.
Described rack 13 includes support 13a, places plate 13b, connecting upright pole 13c, lasso 13d, described placement plate
13b is arranged on support 13a, and described placement plate 13b is equipped with connection provided with 5 putting holes, and each on the right side of putting hole
Vertical rod 13c, is flexibly connected, described lasso 13d is arranged at connecting upright pole between described connecting upright pole 13c and placement plate 13b
13c top, the described lower section for placing plate 13b is provided with Storage water tank, and the described lower section for placing plate 13b is provided with Storage water tank, institute
The bottom for the Storage water tank stated is provided with collector nozzle.
The first described bacteria gathering device is positioned over the putting hole for placing plate 13b.
The second described bacteria gathering device is positioned over lasso 13d.
Operationally, the body of suction hose 5 is connected into peristaltic pump 7 to described suction hose 5.
The described side of control box 1 is provided with the first carry part 15, the second carry part 16, the first described carry part 15 and hung
Provided with solid alcohol lamp, microincubator is linked with the second described carry part 16.
Described control box 1 is provided with handle 18.
Bottom surface in described control box 1, which is provided with pipe clamp standing groove, described pipe clamp standing groove, is placed with pipe clamp 21.
Described sample bucket 3a bucket wall is provided with 50ml, 100ml, 150ml, 200ml, 250ml, 300ml containing mark.
Described syringe 4a barrel is provided with 0.5ml, 1ml, 2ml, 3ml, 4ml, 5ml containing mark.
The length of described suction hose 5 is 140 ~ 200cm.
The length of the described syringe needle 6 that draws water is 15 ~ 20cm.
Protective cap 17 is arranged with the described syringe needle 6 that draws water.
Geometrical clamp is provided with described suction hose 5.
Described observation window 9 is windowpane.
The material of described blocking cap is rubber.
Described gloves are water-proof gloves.
Described collector nozzle is provided with lower water tube hose 14.
The described side for placing plate 13b is provided with 5 tag boards 11, and each tag board 11 corresponds to a collection bacterium dress respectively
Put.
Described the first bacteria gathering device, the second bacteria gathering device remove sealing using being preceding disposably to pack when using
Packaging.
Described the first bacteria gathering device, the second bacteria gathering device can sterilize reuse.
The described side of control box 1 is provided with the 3rd carry part 16, and the 3rd described carry part 7 is used for carry peristaltic pump.
Operation principle and the course of work of the present invention：Rack is taken out out of control box 1 first, then wiped with cotton ball soaked in alcohol
The mouth of a river of fill water 20 is wiped, and alcolhol burner is lighted near the syringe needle 6 that draws water；Protective cap 17 is removed, the syringe needle 6 that will draw water is to be detected
The inside of fill water 20 is inserted at the mouth of a river of fill water 20, turns down rubber cuff 12b, rubber cuff 12b is sealed into fill water 20
The mouth of a river, or adhesive sticker ring plate is bonded to the mouth of a river of fill water 20；Rack 13 is taken out out of control box 1, by the first bacteria gathering device,
Second bacteria gathering device, which removes, to pack, and is reloaded into rack 13, and connect suction hose 5；First bacteria gathering device is entered with pipe clamp 21
Suction hose 5 at the mouth of a river is clamped, and then starts water inlet, sample water flow to the syringe 4a of the second bacteria gathering device, during to assigned scale
Stop water inlet；Then pipe clamp 21 clamps the suction hose 5 at the water inlet tap 4d of the second bacteria gathering device, and sample water enters to the first collection
In the sample bucket 3a of bacterium device, during to assigned scale, stop water inlet；Then bucket seat 3b water draining beak 3c blocking cap is pulled out, and
Blocking cap stopper is entered sample bucket 3a steam vent；Then the sample water started in draining, the first bacteria gathering device is leaked to Sheng from water draining beak
Tank, and discharged from collector nozzle；
When needing to be cultivated, the first bacteria gathering device, the second bacteria gathering device, rack are put into control box 1 simultaneously, operating personnel
Hand stretches into gathering hole 10 and is inserted in gloves, then turns on the first bacteria gathering device, and collection bacterium filter membrane 3d is taken out and delivers to culture dish；Collect bacterium by second
Device is removed and is directed at culture dish, promotes piston push rod 4b, and syringe 4a sample water is pressed out in culture dish by faucet 4c
Entering culture medium can be cultivated；
It is preferred that, when suction hose 5 works, the body of suction hose 5 is connected into peristaltic pump 7, by peristaltic pump 7 by fill water 20
Water is extracted out.
Advantages of the present invention：The present invention is easy to carry, and directly can be carried out in situ sampling, collection bacterium in water factory to be checked, not only
It can not be limited by use environment condition, and detection cycle can be effectively shortened；The present invention is enclosed construction, can be with
Effectively prevent from being contaminated, the accuracy of effective guarantee collection bacterium process；The present invention can be cultivated by different bacterium to be needed, and water sample is evacuated to
In first bacteria gathering device, the second bacteria gathering device, solving needs the shortcoming of multiple sampling during traditional detection, is easy to the later stage to difference
Bacterium classification culture；The present invention can extract filling water sample, with easy to operate, collect bacterium efficiency high, substantially reduce hand labor
The advantage of intensity；The present invention outside can also carry often use detection device, have the advantages that using scope it is wide.
Claims (10)
1. a kind of portable collection bacterium culture systems examined soon for filling drinking water microorganism, it is characterized in that：Including control box
（1）, chamber door（2）, five collection bacterium device, uviol lamps（8）, power supply box, described chamber door（2）Located at control box（1）Side, institute
The control box stated（1）Front be provided be used for check control box（1）The observation window of built-in function（9）, described control box（1）'s
Front is provided with gathering hole（10）, described gathering hole（10）On be provided with gloves, the running-on edge and gathering hole of described gloves
（10）Edge seal connection, described collection bacterium device is arranged at control box（1）Interior, described collection bacterium device includes the first collection bacterium
Device, the second bacteria gathering device, suction hose（5）, draw water syringe needle（6）, suction hose is connected with the top of the first described bacteria gathering device
（5）, described suction hose（5）Water inlet end connection draw water syringe needle（6）, the second described bacteria gathering device is soft with drawing water by flexible pipe
Pipe（5）Top connection, described uviol lamp（8）Located at control box（1）Interior top, described power supply box is located at control box
（1）Outside.
2. the portable quick collection fungus strain system according to claim 1 examined soon for filling drinking water microorganism, its feature
It is：The first described bacteria gathering device includes sample bucket（3a）, bucket seat（3b）, described sample bucket（3a）With bucket seat（3b）Between pass through
Helicitic texture is flexibly connected, described sample bucket（3a）Top be provided with water inlet, steam vent, described bucket seat（3b）Bottom
For concave inward structure, described bucket seat（3b）Bottom centre be provided with water draining beak（3c）, described bucket seat（3b）It is interior to be filtered provided with collection bacterium
Film（3d）, described suction hose（5）Water outlet one end and sample bucket（3a）Water inlet connection, described water draining beak（3c）If
There are blocking cap, described sample bucket（3a）Steam vent bottom be provided with air filter film.
3. the portable quick collection fungus strain system according to claim 1 examined soon for filling drinking water microorganism, its feature
It is：The second described bacteria gathering device includes syringe（4a）, piston push rod（4b）, described syringe（4a）Bottom be provided with faucet
（4c）, described piston push rod（4b）A part be located at syringe（4a）Interior, described syringe（4a）The side at middle part be provided with
Water inlet tap（4d）, described water inlet tap（4d）Relative syringe（4a）Opposite side be provided with ventilation nozzle（4e）, described water inlet tap
（4d）Pass through flexible pipe and suction hose（5）Top connection.
4. the portable quick collection fungus strain system according to claim 1 examined soon for filling drinking water microorganism, its feature
It is：Described suction hose（5）The body of water inlet end be arranged with closing device.
5. the portable quick collection fungus strain system according to claim 4 examined soon for filling drinking water microorganism, its feature
It is：Described closing device includes fixed wafer（12a）, rubber cuff（12b）, described fixed wafer（12a）It is sheathed on and takes out
Water tube hose（5）Water inlet end body, described rubber cuff（12b）With fixed wafer（12a）Edge consolidation, described consolidates
Determine disk（12a）It is provided with ventilation plug（12c）.
6. the portable quick collection fungus strain system according to claim 4 examined soon for filling drinking water microorganism, its feature
It is：Described closing device includes fixed wafer（12a）, adhesive sticker ring plate, described fixed wafer（12a）Be sheathed on draw water it is soft
Pipe（5）Water inlet end body, described adhesive sticker ring plate is located at fixed wafer（12a）Bottom surface, described fixed wafer
（12a）It is provided with ventilation plug（12c）.
7. the portable quick collection fungus strain system according to claim 1 examined soon for filling drinking water microorganism, its feature
It is：Described control box（1）It is interior to be provided with rack（13）, described the first bacteria gathering device, the second bacteria gathering device are located at rack
（13）On.
8. the portable quick collection fungus strain system according to claim 7 examined soon for filling drinking water microorganism, its feature
It is：Described rack（13）Including support（13a）, place plate（13b）, connecting upright pole（13c）, lasso（13d）, described puts
Put plate（13b）It is arranged at support（13a）On, described placement plate（13b）Provided with 5 putting holes, and the right side of each putting hole
It is equipped with connecting upright pole（13c）, described connecting upright pole（13c）With placing plate（13b）Between be flexibly connected, described lasso
（13d）It is arranged at connecting upright pole（13c）Top, described placement plate（13b）Lower section be provided with Storage water tank, described placement plate
（13b）Lower section be provided with Storage water tank, the bottom of described Storage water tank is provided with collector nozzle.
9. the portable quick collection fungus strain system according to claim 1 examined soon for filling drinking water microorganism, its feature
It is：Described suction hose（5）Operationally, suction hose（5）Body is connected into peristaltic pump（7）.
10. the portable quick collection fungus strain system according to claim 1 examined soon for filling drinking water microorganism, its feature
It is：Described control box（1）Side is provided with the first carry part（15）, the second carry part（16）, described the first carry part（15）
On be linked with solid alcohol lamp, the second described carry part（16）On be linked with microincubator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710244026.4A CN107083324B (en) | 2017-04-14 | 2017-04-14 | Portable rapid bacterium collecting system for rapidly detecting microorganisms in filled drinking water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710244026.4A CN107083324B (en) | 2017-04-14 | 2017-04-14 | Portable rapid bacterium collecting system for rapidly detecting microorganisms in filled drinking water |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107083324A true CN107083324A (en) | 2017-08-22 |
| CN107083324B CN107083324B (en) | 2020-03-06 |
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ID=59611685
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710244026.4A Active CN107083324B (en) | 2017-04-14 | 2017-04-14 | Portable rapid bacterium collecting system for rapidly detecting microorganisms in filled drinking water |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111808734A (en) * | 2020-07-01 | 2020-10-23 | 深圳市核子基因科技有限公司 | Bacterial colony sampling device for biotechnology |
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|---|---|---|---|---|
| CN2147894Y (en) * | 1992-11-19 | 1993-12-01 | 倪卫菊 | Disposable millipore filter |
| CN2332725Y (en) * | 1996-11-04 | 1999-08-11 | 叶大林 | Bacteria gathering culture dish |
| WO2003072807A2 (en) * | 2002-02-28 | 2003-09-04 | Houghton Associates | Sterility testing apparatus |
| CN202061628U (en) * | 2011-04-18 | 2011-12-07 | 浙江工业职业技术学院 | Glove box type biosafety cabinet |
| CN202430211U (en) * | 2011-12-28 | 2012-09-12 | 芜湖先声中人药业有限公司 | Culture box support applicable to storing bacterium collectors |
-
2017
- 2017-04-14 CN CN201710244026.4A patent/CN107083324B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2147894Y (en) * | 1992-11-19 | 1993-12-01 | 倪卫菊 | Disposable millipore filter |
| CN2332725Y (en) * | 1996-11-04 | 1999-08-11 | 叶大林 | Bacteria gathering culture dish |
| WO2003072807A2 (en) * | 2002-02-28 | 2003-09-04 | Houghton Associates | Sterility testing apparatus |
| CN202061628U (en) * | 2011-04-18 | 2011-12-07 | 浙江工业职业技术学院 | Glove box type biosafety cabinet |
| CN202430211U (en) * | 2011-12-28 | 2012-09-12 | 芜湖先声中人药业有限公司 | Culture box support applicable to storing bacterium collectors |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111808734A (en) * | 2020-07-01 | 2020-10-23 | 深圳市核子基因科技有限公司 | Bacterial colony sampling device for biotechnology |
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| Publication number | Publication date |
|---|---|
| CN107083324B (en) | 2020-03-06 |
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