CN111807623A - Method for preparing composite carbon source for sewage treatment denitrification by using agricultural products - Google Patents
Method for preparing composite carbon source for sewage treatment denitrification by using agricultural products Download PDFInfo
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- CN111807623A CN111807623A CN202010679955.XA CN202010679955A CN111807623A CN 111807623 A CN111807623 A CN 111807623A CN 202010679955 A CN202010679955 A CN 202010679955A CN 111807623 A CN111807623 A CN 111807623A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/02—Treatment of water, waste water, or sewage by heating
- C02F1/04—Treatment of water, waste water, or sewage by heating by distillation or evaporation
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/38—Treatment of water, waste water, or sewage by centrifugal separation
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
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Abstract
The invention discloses a method for preparing a composite carbon source for sewage treatment denitrification by using agricultural products, which comprises the following steps: 1) pre-treating, namely crushing and grinding crops into powder, adding pure water for pulping, and sterilizing the pulp water to prepare a biomass raw material for later use; 2) adding strains: adding strains into the biomass raw material; 3) anaerobic fermentation: putting the biomass raw material added with the strain into an anaerobic fermentation tower, and adding corresponding enzyme; 4) solid-liquid separation and purification: separating the product of the anaerobic fermentation by a centrifugal separator, returning solid residues to an anaerobic fermentation tower to be reserved as strains, and evaporating and concentrating separated liquid; 5) addition of additives: and adding methanol with the mass of 0.5-1% of the separating liquid into the concentrated separating liquid to obtain the required carbon source product. The invention is specially customized for removing nitrogen by a biochemical method in sewage treatment, and a safe, green, stable and high-efficiency novel carbon source can be extracted by the invention.
Description
Technical Field
The invention relates to the technical field of sewage treatment, in particular to a preparation method of a composite carbon source required to be used in a denitrification process.
Background
Because the urbanization process is continuously accelerated, the discharge amount of domestic sewage and eutrophic substances are increased, and the eutrophication of lakes and reservoirs is increasingly serious. At present, various related departments require that a sewage treatment plant firstly utilizes biological nitrogen and phosphorus removal and then can discharge sewage into a receiving water body to prevent environment pollution. The nitrification and denitrification are efficient biological denitrification technologies, and are widely applied to the field of sewage treatment at present. In the denitrification of microorganisms, heterotrophic denitrifying bacteria consume additional organic matter as a carbon source and provide energy for denitrification. The existing sewage treatment plants in China, particularly the sewage treatment plants in southern areas in China and industrial sewage treatment plants generally have the problem of denitrification efficiency reduction caused by insufficient denitrification carbon sources, which becomes an important factor for restricting the biological denitrification efficiency, so that the requirement of applying an external carbon source to meet the denitrification electron donor needs to be considered.
The variety of external carbon sources is various, and currently, the commonly used external carbon source mainly comprises: methanol, glucose, sodium acetate, some industrial waste products, and the like. When methanol is used as a carbon source, although the operation cost is relatively low, the methanol is a chemical agent, has a certain toxic action, is flammable and explosive, and has poor safety performance; glucose is a multi-molecular saccharide, and when the glucose is added as a carbon source, the denitrification rate is greatly influenced by water quality, so that the mass propagation of bacteria is easily caused, and the water quality of effluent is influenced; sodium acetate is mostly liquid, the COD content is low, the adding amount is large, the transportation cost is high, and the liquid sodium acetate has high water content and high solidification point, so that the sodium acetate is not suitable for being added in northeast China in winter.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a method which is specially customized for removing nitrogen by a biochemical method in sewage treatment, takes agricultural products as raw materials, and is decomposed and extracted by anaerobic fermentation microorganisms to obtain a safe, green, stable and high-efficiency novel carbon source.
The technical scheme adopted by the invention is as follows:
a method for preparing a composite carbon source for sewage treatment denitrification by using agricultural products is characterized by comprising the following steps:
1) pretreatment, namely crushing and grinding crops into powder, adding pure water for pulping, and then sterilizing the pulp water by adopting high-pressure steam to prepare a biomass raw material for later use;
2) adding strains: adding strains into the biomass raw material;
3) anaerobic fermentation: putting the biomass raw material added with the strain into an anaerobic fermentation tower, controlling the temperature of the reaction tower to be kept at 35 +/-2 ℃, adding corresponding enzyme, performing anaerobic fermentation, and entering the next procedure when the COD content in the supernatant generated by fermentation in the fermentation tower is accumulated to 40-50 ten thousand mg/L;
4) solid-liquid separation and purification: separating the product of the anaerobic fermentation by a centrifugal separator, returning solid residues to an anaerobic fermentation tower to be reserved as strains, and concentrating the separated liquid by concentration and evaporation equipment, wherein the COD content in the concentrated separated liquid is 100-;
5) addition of additives: and adding methanol with the mass of 0.5-1% of the separating liquid into the concentrated separating liquid to obtain the required carbon source product.
The agricultural product is soybean, corn, potato, etc
The strain is a mixture of bacillus amyloliquefaciens, aerogenic bacillus and lipolytic bacteria.
The enzyme is a mixture of amylase, proteolytic enzyme, and lipolytic enzyme.
The adding amount of the strain is 0.1 percent of the weight of the biomass raw material.
The amount of enzymatic addition was 0.01% by weight of biomass feedstock.
The invention has the following beneficial effects:
in the invention, the raw materials in the step 1) are agricultural products, and the process used in the step 3) is refined by products decomposed by anaerobic fermentation microorganisms, so that the raw materials are free of corrosion, flammability and danger; the liquid is convenient for storage, transportation and addition.
The main function of the added strain in the step 2) in the invention is to carry out biological dissolution and biological degradation on starch, protein, fat and cellulose. Under the action of bacteria and enzyme, protein, fat, glucose and aromatic hydrocarbon in the raw materials are converted into short-chain organic alcohol, short-chain carbohydrate and the like, which are carbon sources or electron donor sources capable of being rapidly utilized by denitrifying bacteria, so that the denitrifying efficiency is high, and the purified COD content can reach 100 ten thousand mg/L.
In step 5) of the invention, 0.5-% 1% methanol is added to prevent the microorganisms from prematurely propagating in the solution, consuming the COD content therein and affecting the quality of the solution, because the prepared solution has high COD. The COD content of the methanol is about 150 ten thousand mg/L, and the addition of the methanol with low concentration can inhibit the early propagation of microorganisms and has no influence on the quality of the novel carbon source.
Detailed Description
The invention will be further illustrated with reference to specific examples:
example 1:
1) pre-treating, namely firstly crushing and grinding 100Kg of soybeans into powder with the particle size range of 1-2mm, adding 200Kg of pure water, stirring and mixing uniformly to prepare pulp, sterilizing for 20-30min by adopting high-pressure steam at the temperature of 120-;
2) adding strains: adding 100g of a mixture of bacillus amyloliquefaciens, bacillus aerogenes and lipolytic bacteria (the respective contents of the three bacteria are respectively 30%, 45% and 25% of the total weight of the mixture) into the biomass raw material;
3) anaerobic fermentation: putting the biomass raw material added with the strain into an anaerobic fermentation tower, controlling the temperature in the reaction tower to be kept at 35 +/-2 ℃, and specially adding 10g of a mixture of amylase, proteolytic enzyme and lipolytic enzyme (the respective contents of the three enzymes are respectively 30%, 45% and 25% of the total weight of the mixture) for improving the strain decomposition efficiency to perform anaerobic fermentation. The total fermentation time is about 120 hours, supernatant after fermentation is taken every 4 hours and detected, and the next procedure is carried out when the COD (chemical oxygen demand) in the fermentation supernatant is accumulated to 40-50 ten thousand mg/L;
4) solid-liquid separation and purification: separating the product of the anaerobic fermentation by a centrifugal separator, returning solid residues to an anaerobic fermentation tower to be reserved as strains, and concentrating the separated liquid in an evaporator at 300 ℃, so that on one hand, other bacteria generated in the anaerobic fermentation process are killed, on the other hand, part of water in the separated liquid is removed by evaporation, and the COD content in the separated liquid is about 100 ten thousand mg/L;
5) addition of additives: adding methanol with the mass of 0.5 percent of the separating liquid into the concentrated separating liquid to obtain the required carbon source product: sample # 1.
Example 2:
1) pre-treating, namely firstly crushing and grinding 100Kg of corn into powder with the particle size range of 1-2mm, adding 200Kg of pure water, stirring and mixing uniformly to prepare pulp, sterilizing for 20-30min by adopting high-pressure steam under the condition of 120-150 ℃, and sterilizing the pulp to prepare a biomass raw material for later use;
2) adding strains: adding 100g of a mixture of bacillus amyloliquefaciens, bacillus aerogenes and lipolytic bacteria (the respective contents of the three bacteria are respectively 75%, 15% and 10% of the total weight of the mixture) into the biomass raw material;
3) anaerobic fermentation: putting the biomass raw material added with the strain into an anaerobic fermentation tower (conventional equipment), controlling the temperature in the reaction tower to be kept at 35 +/-2 ℃, and specially adding 10g of a mixture of amylase, proteolytic enzyme and lipolytic enzyme (the contents of the three enzymes are respectively 75%, 15% and 10% of the total weight of the mixture) for improving the strain decomposition efficiency to perform anaerobic fermentation. The total fermentation time is about 120 hours, supernatant after fermentation is taken every 4 hours for detection, and the next procedure is carried out when the COD (chemical oxygen demand) in the supernatant after fermentation is accumulated to 40-50 ten thousand mg/L
4) Solid-liquid separation and purification: separating the product of the anaerobic fermentation by a centrifugal separator, returning solid residues to an anaerobic fermentation tower to be reserved as strains, and concentrating the separated liquid by evaporation concentration equipment, so that on one hand, other bacteria generated in the anaerobic fermentation process are killed, on the other hand, part of water in the separated liquid is removed by evaporation, and the COD content in the separated liquid is about 100 ten thousand mg/L;
5) addition of additives: adding methanol with the mass of 1% of the separating liquid into the concentrated separating liquid to obtain the required carbon source product: sample # 2.
Example 3:
1) pre-treating, namely firstly crushing and grinding 100Kg of peanuts into powder with the particle size range of 1-2mm, adding 200Kg of pure water, stirring and mixing uniformly to prepare pulp, sterilizing for 20-30min by adopting high-pressure steam at the temperature of 120-;
2) adding strains: adding 100g of a mixture of bacillus amyloliquefaciens, bacillus aerogenes and lipolytic bacteria (the respective contents of the three bacteria are respectively 20%, 35% and 45% of the total weight of the mixture) into the biomass raw material;
3) anaerobic fermentation: the biomass raw material added with the strain is put into an anaerobic fermentation tower (the conventional equipment is available), the temperature in the reaction tower is controlled to be kept at 35 +/-2 ℃, and in order to improve the strain decomposition efficiency, 10g of a mixture of amylase, proteolytic enzyme and lipolytic enzyme (the contents of the three enzymes are respectively 20%, 35% and 45% of the total weight of the mixture) is specially added for anaerobic fermentation. The total fermentation time is about 120 hours, wherein fermentation supernatant is taken every 4 hours for detection, and the next procedure is carried out when the COD (chemical oxygen demand) in the fermentation supernatant is accumulated to 40-50 ten thousand mg/L;
4) solid-liquid separation and purification: separating the product of the anaerobic fermentation by a centrifugal separator, returning solid residues to an anaerobic fermentation tower to be reserved as strains, and concentrating the separated liquid by evaporation concentration equipment, so that on one hand, other bacteria generated in the anaerobic fermentation process are killed, on the other hand, part of water in the separated liquid is removed by evaporation, and the COD content in the separated liquid is about 100 ten thousand mg/L;
5) addition of additives: adding methanol with the mass of 0.8% of the separating liquid into the concentrated separating liquid to obtain the required carbon source product: sample # 3.
The application effect of the invention is illustrated as follows:
in order to verify the effect of the invention, an industrial wastewater treatment plant from Sichuan is taken as a test implementation object, the COD concentration of inlet water of the industrial wastewater treatment plant is about 120mg/L, the total nitrogen concentration is about 50mg/L, the ammonia nitrogen concentration is about 50mg/L, the C/N ratio of the inlet water is disordered, and a proper amount of external carbon source is required to be added to complete the denitrification process. A comparative test was carried out using 5 pilot-scale denitrification test units with a throughput of 0.5m3/h。
Sodium acetate and glucose are used as a comparison group; the samples No. 1, No. 2 and No. 3 of the novel denitrification carbon source in the embodiment examples 1-3 are test groups. And (3) preparing the 5 carbon sources into carbon sources with the same COD concentration, and equivalently adding the carbon sources into a pilot-scale denitrification experimental device. The removal effects of the 2 control groups and the 3 test groups are shown in Table 1
Table 1:
according to the data, the effect of total nitrogen and ammonia nitrogen of pilot-scale water using the carbon source of the invention is better than that of a comparison group using sodium acetate and glucose under the condition of adding the carbon source with the same COD content.
Other undescribed portions of the present invention are the same as the prior art.
Claims (6)
1. A method for preparing a composite carbon source for sewage treatment denitrification by using agricultural products is characterized by comprising the following steps:
1) pretreatment, namely crushing and grinding crops into powder, adding pure water for pulping, and then sterilizing the pulp water by adopting high-pressure steam to prepare a biomass raw material for later use;
2) adding strains: adding strains into the biomass raw material;
3) anaerobic fermentation: putting the biomass raw material added with the strain into an anaerobic fermentation tower, controlling the temperature of the reaction tower to be kept at 35 +/-2 ℃, adding corresponding enzyme, performing anaerobic fermentation, and entering the next procedure when the COD content in the supernatant generated by fermentation in the fermentation tower is accumulated to 40-50 ten thousand mg/L;
4) solid-liquid separation and purification: separating the product of the anaerobic fermentation by a centrifugal separator, returning solid residues to an anaerobic fermentation tower to be reserved as strains, and concentrating the separated liquid by concentration and evaporation equipment, wherein the COD content in the concentrated separated liquid is 100-;
5) addition of additives: and adding methanol with the mass of 0.5-1% of the separating liquid into the concentrated separating liquid to obtain the required carbon source product.
2. The method for preparing a composite carbon source for denitrification of sewage treatment using agricultural products according to claim 1, wherein the agricultural products are soybeans, corns or peanuts.
3. The method for preparing a composite carbon source for denitrification of sewage treatment using agricultural products according to claim 1 or 2, wherein the bacterial species is a mixture of bacillus amyloliquefaciens, aerobacter aerogenes, and lipolytic bacteria.
4. The method for preparing a composite carbon source for denitrification of sewage treatment using agricultural products according to claim 1 or 2, wherein the enzyme is a mixture of amylase and lipolytic enzyme.
5. The method for preparing a composite carbon source for denitrification of sewage treatment by using agricultural products as claimed in claim 3, wherein the amount of the added strain is 0.1% by weight of the biomass raw material.
6. The method for preparing a composite carbon source for denitrification of sewage treatment using agricultural products according to claim 4, wherein the enzymatic addition is 0.01% by weight of biomass raw material.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112850912A (en) * | 2020-12-29 | 2021-05-28 | 南京尚迪纳米科技有限公司 | Biological composite carbon source and preparation method and application thereof |
| CN113548769A (en) * | 2021-07-13 | 2021-10-26 | 江苏金山环保科技有限公司 | Method for preparing biological composite carbon source by using citric acid fermentation wastewater and application |
| CN113772814A (en) * | 2021-10-11 | 2021-12-10 | 深圳市深水水务咨询有限公司 | Efficient multi-dimensional carbon source and preparation method thereof |
| CN114395480A (en) * | 2021-12-29 | 2022-04-26 | 江苏力鼎环保装备有限公司 | Carbon source for microbial culture applied to sewage treatment and application thereof |
| CN115465958A (en) * | 2022-09-26 | 2022-12-13 | 武汉森泰环保股份有限公司 | Sewage denitrification biological nutrient and preparation method and device thereof |
| CN117361748A (en) * | 2023-11-28 | 2024-01-09 | 山东绿邦生物科技有限公司 | Carbon source for wastewater denitrification treatment, and preparation method and application thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112850912A (en) * | 2020-12-29 | 2021-05-28 | 南京尚迪纳米科技有限公司 | Biological composite carbon source and preparation method and application thereof |
| CN113548769A (en) * | 2021-07-13 | 2021-10-26 | 江苏金山环保科技有限公司 | Method for preparing biological composite carbon source by using citric acid fermentation wastewater and application |
| CN113772814A (en) * | 2021-10-11 | 2021-12-10 | 深圳市深水水务咨询有限公司 | Efficient multi-dimensional carbon source and preparation method thereof |
| CN113772814B (en) * | 2021-10-11 | 2022-11-18 | 深圳市深水水务咨询有限公司 | Efficient multi-dimensional carbon source and preparation method thereof |
| CN114395480A (en) * | 2021-12-29 | 2022-04-26 | 江苏力鼎环保装备有限公司 | Carbon source for microbial culture applied to sewage treatment and application thereof |
| CN115465958A (en) * | 2022-09-26 | 2022-12-13 | 武汉森泰环保股份有限公司 | Sewage denitrification biological nutrient and preparation method and device thereof |
| CN115465958B (en) * | 2022-09-26 | 2023-08-08 | 武汉森泰环保股份有限公司 | Biological nutrient for sewage denitrification and preparation method and device thereof |
| CN117361748A (en) * | 2023-11-28 | 2024-01-09 | 山东绿邦生物科技有限公司 | Carbon source for wastewater denitrification treatment, and preparation method and application thereof |
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