A kind of compound carbon source of multinuclear for sewage disposal strengthened denitrification
Technical field
The invention belongs to sewage treatment plant's biochemistry section denitrification process additional carbon technical fields, and in particular to one kind is used for
The additional multinuclear compounded carbons of sewage treatment plant's biochemistry section strengthened denitrification.
Background technology
With Chinese society expanding economy, the increase of population and the increase of scope of the enterprise, sewage drainage amount increasingly increase
It is more, therefore exploitation is very important for the technology and medicament of wastewater efficient processing.It uses based on activated sludge process
It is current domestic or even sewage treatment plant of the world mainstream technology that biochemistry, which denitrogenates technique, and principle is thin using denitrification in sewage
Bacterium restores nitrate under anoxic conditions, releases molecular nitrogen (N2) or nitrous oxide (N2O method) removes total nitrogen.Greatly
Part denitrifying bacteria is heterotroph, needs carbon source as the energy needed for cell activities in denitrification process, carries out
Anaerobic respiration, to remove the total nitrogen in sewage.
Domestic sewage plant influent COD value, BOD values are generally relatively low, often need to add carbon source, additional carbon in denitrification process
Be C/N relatively low (COD/ total nitrogens) biochemical wastewater treatment in essential process, carbon source content can restrict heterotrophic denitrification
Remove efficiency and the effect of total nitrogen.
The common additional carbon of sewage treatment plant has methanol, sodium acetate and glucose etc..Methanol is due to its molecule little Yi quilts
Microbial metabolism, denitrification rate height are ideal carbon sources, but because its high toxicity, inflammable and explosive, transportation safety and at
This problems such as, limits its extensive use.For sodium acetate as carbon source, denitrification rate is very fast, and existing market is most widely used,
But of high cost is still its restrictive condition.Glucose is as carbon source, and denitrification rate is slower with respect to sodium acetate and methanol, but its COD
Virtual value is high, is still generally being used as important carbon source, processing cost is more slightly lower than sodium acetate.
The carbon source that sewage treatment plant uses at present not only has that dosage is big, operating cost is high.Due to anti-nitre
The characteristics of changing bacterial diversity, such as the composite floras condition such as denitrogenation micrococcus, denitrifying pseudomonas, under single carbon source environment
Bacterial diversity is weakened, and artificial reduction, the growth of domestication interference flora and activity, denitrification process do not give full play to instead
The total nitrogen removal efficiency of nitrifying bacteria community.
Denitrifying bacterium plays conclusive effect, growth, breeding as microorganism during sewage treatment plant denitrogenates
Decide the activity and efficiency of denitrification denitrogenation with various physiological metabolisms.The nutriment of microorganism is the life for meeting microorganism
It is long, breed and complete various physiological metabolisms it is dynamic necessary to element, Institute of Micro-biology needs nutriment that can be divided into six major class, i.e., water,
Carbon source, nitrogen source, inorganic salts, growth factor and the energy.Most of denitrifying bacterium belongs to heterotrophic bacteria, other are not needed other than carbon source
The energy;Simultaneously containing inorganic salts such as certain phosphorus, sodium, calcium in sewage, other micronutrient levels of microorganism demand are less, and
Seldom contain and is with or without the prebiotic factor of microorganism.
Invention content
The present invention is to solve the additional common carbon source sodium acetate of current sewage treatment plant's biochemistry section strengthened denitrification, glucose
That there are dosages is big, operating cost is high, adds the problems such as single carbon source can weaken the denitrifying capacity of denitrification flora for a long time, main
Denitrification bacterial activity and denitrification are improved in terms of addition Institute of Micro-biology needs carbon source kind, inorganic salts and the prebiotic factor of growth
Rate, provide it is a kind of it is at low cost, environmentally protective, dosage is less, COD virtual values are high, reversed digestive efficiency is high, be conducive to strengthen bacterium
Group's diversity, while the multinuclear compound carbon source good to water quality and flora applicability.
The compound carbon source of multinuclear is prepared by following methods used by solving above-mentioned technical problem:
Starch is added in 70~100 DEG C of hot water, stirring is so that starch fluid is fully gelatinized, then under stirring
Starch Hydrolysis catalyst and acetic acid are added, is hydrolyzed 1~2 hour at 80~100 DEG C, be cooled to 50 DEG C or less and alkali is added to molten
Liquid pH value is 5.5~7.0, then adds the prebiotic factor of microorganism, vitamin and minerals, stirs evenly, filter and decolourize, i.e.,
Obtain the compound carbon source of multinuclear.
In the preparation method of the above-mentioned compound carbon source of multinuclear, the additive amount of the starch account for whole system quality 10%~
35% (is with starch, hot water, Starch Hydrolysis catalyst, the prebiotic factor of acetic acid microorganism, vitamin and minerals gross mass
100% meter);The Starch Hydrolysis catalyst is any one in sulfuric acid, hydrochloric acid, and additive amount accounts for the 1% of whole system quality
~2%;The additive amount of the acetic acid accounts for the 1%~5% of whole system quality;The prebiotic factor of microorganism is yeast extract, egg
Any one in white peptone or two kinds of mixture, additive amount account for the 0.01%~0.5% of whole system quality;The vitamin
For vitamin B1, vitamin B12In any one or two kinds mixture, additive amount account for whole system quality 0.001%~
0.01%;The minerals are magnesium sulfate and potassium sulfate, and additive amount accounts for the 0.002%~0.02% of whole system quality;Institute
It is any one in sodium hydroxide, sodium carbonate to state alkali.
In the preparation method of the above-mentioned compound carbon source of multinuclear, it is filtered using filtering bag filter, is filtered using activated carbon
Device decolourizes.
The compound carbon source of multinuclear of the present invention contains the carbon source needed for different microorganisms bacterial metabolism, including Starch Hydrolysis list
Sugar, disaccharides, short chain carboxy acid, short chain alcohol and compounding carbon source acetic acid, so as to avoid the bacterial diversity under single carbon source environment
Reduction, enhances bacterial diversity and activity;Acetic acid more has as amylolytic co-catalyst and sugared open loop accelerating agent simultaneously
It is used as carbon metabolism conducive to sugar;Needed for the micro- magnesium sulfate of addition, the sulphur in potassium sulfate, potassium, magnesium are grown as microorganism
And the macroelement lacked in sewage, it is very useful to the growth of microorganism;Meanwhile magnesium is the active group of certain enzymes in cell
And activator, it is that the indispensable constituent of microbial cell structural material and microorganism grow indispensable nutrients
Matter further improves breeding and the activity of denitrification flora;The prebiotic factor of microorganism is that microorganism maintains normal activities
The indispensable special organic nutrient substance of institute, these substance microorganisms cannot synthesize itself, and directly affect various enzymes, flora
Activity, the prebiotic factor of microorganism lacked by adding vitamin, yeast extract, peptone, in these sewage and conventional carbon source,
The bioactivity of denitrification flora can be effectively improved, while more efficient, comprehensive carbon source being provided for denitrification again.
Beneficial effects of the present invention are as follows:
1, the compound carbon source of multinuclear of the present invention belongs to the compound carbon source of small molecule, is conducive to strengthen bacterial diversity, simultaneously
It is good to water quality and flora applicability.
2, the compound carbon source of multinuclear of the present invention contains the micro- and prebiotic factor that Institute of Micro-biology needs, bioactivity, anti-
To digestive efficiency height, so carbon source dosage is less.
3, for the compound carbon source of multinuclear of the present invention using hydrolysis sugar as key component carbon source, effective COD value is 100,000~350,000,
COD virtual values are high, and denitrification usability is good.
4, the compound carbon source of multinuclear of the present invention is using starch as main raw material(s), at low cost and compared with chemical syntheses such as sodium acetates
Carbon source is more environmentally protective, nontoxic, and manufacture craft is easy.
5, the compound carbon source of multinuclear of the present invention can under the environment of biochemical section water carbon source deficiency effective supplementary carbon source, be
Anaerobic system starts or operation provides efficient microbe carbon source;It can start in Industrial Waste Water Treatments biochemistry section and run, is biochemical
System loading declines, improves biochemistry microbial metabolism environment B/C ratios, promotes biochemical system impact resistance and toxicity impact capacity
When efficient carbon source is provided.
Specific implementation mode
With reference to embodiment, the present invention is described in more detail, but protection scope of the present invention is not limited only to these realities
Apply example.
Embodiment 1
100g starch is added in the hot water of 70 DEG C of 880g, stirring is so that starch fluid is fully gelatinized, then in stirring shape
10g hydrochloric acid and 10g acetic acid are added under state, are hydrolyzed 1 hour at 80 DEG C, are cooled to 50 DEG C hereinafter, and NaOH is added to pH value of solution
Between value is 5.5~7,0.1g yeast extracts, 0.01g vitamin Bs is then added1、0.01g MgSO4、0.01g K2SO4, stirring is
It is even, it is decolourized to get to the compound carbon source of multinuclear with active carbon filter after being filtered with filtering bag filter.
Embodiment 2
200g starch is added in the hot water of 70 DEG C of 770g, stirring is so that starch fluid is fully gelatinized, then in stirring shape
10g hydrochloric acid and 20g acetic acid are added under state, are hydrolyzed 1 hour at 90 DEG C, are cooled to 50 DEG C hereinafter, and NaOH is added to pH value of solution
Between value is 5.5~7,0.5g peptones, 0.03g vitamin Bs is then added12、0.03g MgSO4、0.03g K2SO4, stirring is
It is even, it is decolourized to get to the compound carbon source of multinuclear with active carbon filter after being filtered with filtering bag filter.
Embodiment 3
300g starch is added in the hot water of 70 DEG C of 645g, stirring is so that starch fluid is fully gelatinized, then in stirring shape
20g sulfuric acid and 35g acetic acid are added under state, are hydrolyzed 1 hour at 95 DEG C, are cooled to 50 DEG C hereinafter, and NaOH is added to pH value of solution
Between value is 5.5~7,2g yeast extracts, 2g peptones, 0.05g vitamin Bs is then added12、0.05g MgSO4、0.05g
K2SO4, stir evenly, decolourized to get to the compound carbon source of multinuclear with active carbon filter after being filtered with filtering bag filter.
Embodiment 4
350g starch is added in the hot water of 70 DEG C of 580g, stirring is so that starch fluid is fully gelatinized, then in stirring shape
20g sulfuric acid and 50g acetic acid are added under state, are hydrolyzed 2 hours at 95 DEG C, are cooled to 50 DEG C hereinafter, and NaOH is added to pH value of solution
Between value is 5.5~7,2.5g yeast extracts, 2.5g peptones, 0.05g vitamin Bs is then added1, 0.05g vitamin Bs12、0.1g
MgSO4、0.1g K2SO4, stir evenly, decolourized to get compound to multinuclear with active carbon filter after being filtered with filtering bag filter
Type carbon source.
In order to prove that beneficial effects of the present invention, inventor are carried out using the compound carbon source of multinuclear prepared by Examples 1 to 4
Denitrification is tested, and specific test situation is as follows:
It chooses certain sewage plant anaerobism section water sample and carries out denitrification experiment, 5000~5500mg/L of sludge concentration tests raw water
Total nitrogen 55mg/L, COD 100mg/L.It takes 1000mL water samples to be stirred at the same speed in six company's blenders, adds Examples 1 to 4 respectively
The glucose that aqueous sodium acetate solution that the compound carbon source of multinuclear of preparation, mass fraction are 25%, mass fraction are 30% is water-soluble
For liquid as additional carbon, the influence to denitrification rate and nitrogen removal rate is shown in Table 1.
Table 1
By the Experimental Comparison result of table 1 it is found that the compound carbon source of multinuclear of the present invention has relative to glucose and sodium acetate
Standby higher denitrification rate and COD utilization rates, denitrification nitrogen removal rate is high, dosage is few;Multinuclear composite carbon of the present invention simultaneously
Source includes more carbon source components, vitamin, minerals, the prebiotic factor of microorganism etc., compared to simple carbon source, the removal of denitrification total nitrogen
Efficiency and ability are provided with apparent technical advantage in short-term and long process.