CN116251819A - Method for producing denitrifying carbon source for sewage treatment by utilizing organic waste residues in food industry - Google Patents
Method for producing denitrifying carbon source for sewage treatment by utilizing organic waste residues in food industry Download PDFInfo
- Publication number
- CN116251819A CN116251819A CN202310125812.8A CN202310125812A CN116251819A CN 116251819 A CN116251819 A CN 116251819A CN 202310125812 A CN202310125812 A CN 202310125812A CN 116251819 A CN116251819 A CN 116251819A
- Authority
- CN
- China
- Prior art keywords
- carbon source
- producing
- denitrification
- organic waste
- food industry
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 79
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 79
- 238000011282 treatment Methods 0.000 title claims abstract description 50
- 239000010815 organic waste Substances 0.000 title claims abstract description 48
- 239000010865 sewage Substances 0.000 title claims abstract description 39
- 235000013305 food Nutrition 0.000 title claims abstract description 36
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 17
- 238000000855 fermentation Methods 0.000 claims abstract description 95
- 230000004151 fermentation Effects 0.000 claims abstract description 46
- 239000010802 sludge Substances 0.000 claims abstract description 32
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 27
- 238000000034 method Methods 0.000 claims abstract description 25
- 239000000047 product Substances 0.000 claims abstract description 20
- 239000002002 slurry Substances 0.000 claims abstract description 18
- 238000001223 reverse osmosis Methods 0.000 claims abstract description 13
- 239000006228 supernatant Substances 0.000 claims abstract description 7
- 241000894006 Bacteria Species 0.000 claims description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 17
- 230000001580 bacterial effect Effects 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 6
- 238000005119 centrifugation Methods 0.000 claims description 4
- 241001331812 Saccharofermentans acetigenes Species 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 2
- 230000008569 process Effects 0.000 abstract description 14
- 230000020477 pH reduction Effects 0.000 abstract description 10
- 230000007062 hydrolysis Effects 0.000 abstract description 6
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 6
- 238000004064 recycling Methods 0.000 abstract description 5
- 239000002910 solid waste Substances 0.000 abstract description 3
- 102000010911 Enzyme Precursors Human genes 0.000 abstract 2
- 108010062466 Enzyme Precursors Proteins 0.000 abstract 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 14
- 230000000789 acetogenic effect Effects 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 7
- 229910052757 nitrogen Inorganic materials 0.000 description 7
- 150000001720 carbohydrates Chemical class 0.000 description 6
- 235000014633 carbohydrates Nutrition 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000002351 wastewater Substances 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 3
- 238000004364 calculation method Methods 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 3
- 238000001125 extrusion Methods 0.000 description 3
- 230000003301 hydrolyzing effect Effects 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000009264 composting Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000010813 municipal solid waste Substances 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- RJGDLRCDCYRQOQ-UHFFFAOYSA-N anthrone Chemical compound C1=CC=C2C(=O)C3=CC=CC=C3CC2=C1 RJGDLRCDCYRQOQ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 229920002988 biodegradable polymer Polymers 0.000 description 1
- 239000004621 biodegradable polymer Substances 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- SOCTUWSJJQCPFX-UHFFFAOYSA-N dichromate(2-) Chemical compound [O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O SOCTUWSJJQCPFX-UHFFFAOYSA-N 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000002289 effect on microbe Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 235000020774 essential nutrients Nutrition 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 238000011197 physicochemical method Methods 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
- B09B3/30—Destroying solid waste or transforming solid waste into something useful or harmless involving mechanical treatment
- B09B3/35—Shredding, crushing or cutting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
- B09B3/30—Destroying solid waste or transforming solid waste into something useful or harmless involving mechanical treatment
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
- B09B3/60—Biochemical treatment, e.g. by using enzymes
- B09B3/65—Anaerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
- C02F3/305—Nitrification and denitrification treatment characterised by the denitrification
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
Landscapes
- Environmental & Geological Engineering (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Water Supply & Treatment (AREA)
- Organic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Treatment Of Sludge (AREA)
Abstract
The invention provides a method for producing a denitrification carbon source for sewage treatment by utilizing organic waste residues in the food industry, and relates to the field of solid waste recycling treatment. The method comprises the following steps: pretreating organic waste residues in the food industry to prepare organic slurry; performing reverse osmosis concentration treatment on the organic slurry to obtain concentrated solution; anaerobic sludge is added into an anaerobic fermentation reactor, concentrated solution is injected into the anaerobic fermentation reactor, and acetic acid sugar producing zymophyte is inoculated in parallel for anaerobic fermentation; and centrifuging the anaerobic fermentation product, and taking supernatant to obtain the denitrification carbon source for sewage treatment. The addition of the zymogen producing acetic acid sugar in the anaerobic fermentation process can obviously promote COD of the denitrification carbon source, improve the hydrolysis acidification efficiency, and the carbon source produced by adding the zymogen can greatly improve the denitrification rate and the denitrification potential in the denitrification application, so that the utilization rate of the carbon source is higher, and the denitrification capability is better. The process has the advantages of simple flow, low economic cost and convenient operation.
Description
Technical Field
The invention relates to the field of solid waste recycling treatment, in particular to a method for producing a denitrifying carbon source for sewage treatment by utilizing organic waste residues in the food industry.
Background
Along with the continuous improvement of the environmental standards in China, the water quality and the water quantity are changed, and the sewage treatment still faces serious challenges. Nitrogen is an essential nutrient for plant growth, but a large amount of nitrogen is discharged into water body to easily cause eutrophication of water body and harm aquatic animals, thus causing serious environmental problems. Therefore, the improvement of the denitrification efficiency of the sewage treatment plant and the reduction of the nitrogen content in the effluent are always the problems of researchers. Nitrogen-containing compounds in sewage can be removed by a physicochemical method or can be converted by biological processes, but biological denitrification technology is most economical and is widely used. The biological denitrification mechanism is that under anaerobic or anoxic conditions, denitrifying bacteria utilize organic matters as a carbon source and energy to reduce nitrate or nitrite into gaseous nitrogen. The carbon source is important for the denitrification process and directly affects the denitrification efficiency. The addition of an external carburetion source, strengthening the denitrification process and improving the denitrification efficiency, has been widely studied.
The external carbon source for enhancing denitrification generally comprises a traditional chemical carbon source, high-concentration organic wastewater, a granular carbon source, anaerobic fermentation products and the like. Traditional chemical carbon sources such as methanol, acetic acid, glucose and the like are easy to be absorbed and metabolized by microorganisms, and energy is rapidly provided to realize the denitrification process. However, researches show that the quality of effluent water is easily deteriorated due to improper addition amount of the carbon source, and the carbon source is expensive and difficult to use in a large scale in the actual sewage treatment process. The high-concentration organic wastewater has rich organic species and higher concentration, but the wastewater has complex components, and the application needs to consider whether the wastewater has adverse effect on microorganisms, whether new pollutants are introduced, whether the effluent quality is deteriorated, and the like. The granular carbon source is natural plant (wheat straw, bark, cotton, reed, etc.) and biodegradable polymer, but the slow-release carbon source has limited denitrification efficiency improvement. Anaerobic fermentation converts complex organic matters in a matrix into small molecular organic matters, such as organic acids, alcohols and the like, which can be rapidly degraded and utilized by microorganisms, provide energy for metabolic activity and show a rapid denitrification rate. The residual sludge of the sewage treatment plant has higher organic matter content, is an ideal substrate for preparing the denitrification carbon source by anaerobic fermentation, is beneficial to reducing the sludge yield, and is widely paid attention to by researchers. However, the sludge hydrolysis process is slow, which seriously affects the subsequent acidification efficiency.
The organic waste residue in food industry is waste matter produced in the process of processing target product with animal and plant as material, and features high content of protein, carbohydrate, fat and other organic matter, high content of suspended solid matter and relatively low toxicity. The organic waste residue in the food industry is used as organic solid waste, and the treatment of the organic waste residue is incorrect and has influence on the environment. The reasonable disposal of the organic waste residue in the food industry is necessary, and the harmless, reduction and recycling principles should be followed. At present, the technology for treating the organic waste residues in the food industry mainly comprises landfill technology, incineration technology, composting technology, feed technology, anaerobic fermentation technology and the like. However, the organic waste residues in the food industry are increased, most of the organic waste residues are only subjected to landfill, incineration and composting treatment, and effective recycling and utilization are not realized, so that serious resource waste and environmental sanitation and safety hazards are caused.
In recent years, related research institutions at home and abroad have made a certain research on the aspect of preparing a carbon source from organic garbage, but the carbon source prepared by the prior art only carries out simple physical crushing and other pretreatment on the organic garbage and then carries out simple hydrolytic acidification, the effective COD equivalent of the prepared carbon source product is not high, the carbon source product belongs to a low-concentration carbon source product, the carbon source product is calculated according to the effective COD equivalent in actual engineering use, and compared with the traditional carbon source product, the carbon source product has larger consumption, is limited by larger transportation radius and has high transportation cost. How to improve the hydrolytic acidification efficiency of producing denitrifying carbon sources by utilizing organic waste residues in the food industry and the COD equivalent of the obtained carbon source products becomes a technical problem to be solved by the technicians in the field.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to overcome the defects of low hydrolytic acidification efficiency and low COD equivalent when the denitrifying carbon source for sewage treatment is produced by utilizing the organic waste residues in the food industry in the prior art, so as to provide the method for utilizing the denitrifying carbon source for sewage treatment by utilizing the organic waste residues in the food industry, which can improve the utilization rate of organic matters, obtain the carbon source with high COD concentration and realize the efficient recovery of the carbon source.
In order to achieve the above purpose, the present invention provides the following technical solutions:
in a first aspect, the invention provides a method for producing a denitrified carbon source for sewage treatment by using organic waste residues in the food industry, comprising the following steps:
(1) Pretreating organic waste residues in the food industry to prepare organic slurry;
(2) Performing reverse osmosis concentration treatment on the organic slurry to obtain concentrated solution;
(3) Anaerobic sludge is added into the anaerobic fermentation reactor, the concentrated solution is injected into the anaerobic fermentation reactor, and acetic acid sugar producing zymophyte is inoculated for anaerobic fermentation;
(4) And centrifuging the anaerobic fermentation product, and taking supernatant to obtain the denitrification carbon source for sewage treatment.
Further, in the step (3), the sugar-producing fermentation bacteria specifically select a sugar-producing fermentation bacteria (Saccharofermentans acetigenes) CGMCC 1.5064.
Further, in the step (3), bacterial liquid of the sugar-producing fermentation bacteria is added in an amount of 20-30% of the volume of the concentrated liquid, and the viable count of the bacterial liquid of the sugar-producing fermentation bacteria is 1.2x10 8 ～1.3×10 8 cfu/L。
Further, in the step (3), the dosage of the anaerobic sludge is 15% -25% of the volume of the concentrated solution; the VSS/TSS of the anaerobic sludge is larger than 0.7, and the sludge concentration is 5000+/-100 mg/L.
Further, in the step (3), the anaerobic fermentation conditions are as follows: the pH value is 8.0-8.5, the temperature is 35-45 ℃ and the time is 36-48 h (fermentation is stopped when the components in the reactor are basically kept stable).
Further, in step (1), the preprocessing includes: crushing the organic waste residues in the food industry, adding hot water, uniformly mixing, and filtering to obtain the organic slurry, wherein the pressure during crushing is controlled to be 0.13-0.15 Mpa; the mass of the added hot water is 2 to 5 times of the mass of the organic waste residue, and the temperature of the hot water is 80 to 90 ℃; the filtering is carried out for 1 to 3 times by using a screen mesh with 10 to 50 meshes.
Further, in the step (2), the reverse osmosis concentration treatment adopts a disc-tube type reverse osmosis membrane; concentrating the organic slurry by 3-5 times through the reverse osmosis concentration treatment.
Further, in the step (4), the centrifugation conditions are: 4000-6000 rpm for 8-12 min.
In a second aspect, the invention provides a denitrification carbon source for sewage treatment prepared by the method.
In a third aspect, the present invention provides a sewage treatment method comprising subjecting sewage to denitrification treatment, wherein a carbon source used in the denitrification treatment is produced by the method of claim.
The technical scheme of the invention has the following advantages:
the invention provides a method for producing a denitrification carbon source for sewage treatment by utilizing organic waste residues in the food industry, which comprises the steps of pretreatment, reverse osmosis concentration treatment, anaerobic fermentation and centrifugation.
According to the invention, high protein substances in the organic waste residue are enriched and trapped in the filter residue through pretreatment, so that the release of nitrogen sources of the organic waste residue in the hydrolysis process can be reduced, the nitrogen source load of a sewage treatment plant is prevented from being increased by adding the nitrogen sources into sewage in the form of carbon sources, the denitrification effect of the product when the product is used for sewage treatment is influenced, most of the organic slurry collected after pretreatment is starch carbohydrate, the starch carbohydrate is easy to degrade by microorganisms, and the utilization rate of organic matters is improved.
The slurry pretreated by the organic waste residue in the food industry is concentrated in high power, so that the concentrated water quantity can be reduced, the organic matters in the organic waste residue are degraded to the maximum extent, the utilization rate of the organic matters is improved, the carbon source product with higher COD equivalent is produced, and the efficient recovery of the carbon source is realized.
According to the invention, anaerobic sludge and acetogenic sugar fermentation bacteria are inoculated in the anaerobic fermentation process, so that the fermentation process is controlled in the hydrolysis, acidification and acetogenic stages, macromolecular organic matters are hydrolyzed and converted into small molecular organic matters which are easy to biodegrade, and Volatile Fatty Acids (VFAs) with high denitrification rate are generated, so that a liquid denitrification carbon source with stable components is obtained. Experimental comparison shows that the addition of the acetogenic sugar fermentation bacteria can obviously promote COD of the denitrification carbon source, improve the hydrolysis acidification efficiency, and the carbon source produced by the addition of the bacteria can greatly improve the denitrification rate and the denitrification potential in the denitrification application, so that the carbon source utilization rate is higher, and the denitrification capability is better.
The method has the advantages of simple process flow, low economic cost and convenient operation, realizes the recycling of the organic waste residues in the food industry, and has good popularization and application prospects.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a flow chart of a process for producing a denitrified carbon source for sewage treatment by using organic waste residues in the food industry in example 1 of the present invention.
Detailed Description
The following examples are provided for a better understanding of the present invention and are not limited to the preferred embodiments described herein, but are not intended to limit the scope of the invention, any product which is the same or similar to the present invention, whether in light of the present teachings or in combination with other prior art features, falls within the scope of the present invention.
The acetogenic sugar fermentation bacteria used in the embodiment and experimental example of the invention are specifically acetogenic sugar fermentation bacteria (Saccharofermentans acetigenes) CGMCC 1.5064, which are purchased from China general microbiological culture Collection center, and the address of a preservation unit: the preservation number of the Beijing city Chaoyang area North Chen Xili No.1 and 3 is CGMCC No.1.5064.
The anaerobic sludge used in the embodiment and experimental example of the invention is anaerobic activated sludge obtained from a sewage treatment plant, the VSS/TSS (ratio of anaerobic bacteria in the sludge) of the anaerobic sludge is more than 0.7, and the sludge concentration is 5000+/-100 mg/L.
DTRO system: LT-S20000, kunshan Weida environmental protection equipments Co., ltd.
Anaerobic fermentation reactor: BL-BJGD, belgium bioengineering equipment (Jiangsu Co., ltd.).
The specific experimental procedures or conditions are not noted in the examples and may be followed by the operations or conditions of conventional experimental procedures described in the literature in this field. The materials or instruments used are all conventional products commercially available, including but not limited to those used in the examples of the present application.
Example 1
The embodiment provides a method for producing a denitrification carbon source for sewage treatment by using organic waste residues in the food industry, wherein the process flow is shown in a figure 1, and the specific operation steps are as follows:
(1) Pretreatment: crushing organic waste residues in the food industry under the extrusion action of 0.15MPa, adding hot water (85 ℃) which is 3 times of the mass of the organic waste residues, uniformly mixing, filtering the mixture through a 10-mesh and 20-mesh screen, controlling the particle size of the organic slurry to be below 0.25mm, and retaining about 46% of protein in the organic waste residues by filter residues;
(2) Reverse osmosis concentration: the organic slurry is conveyed to a DTRO system through a conveying belt by a high-pressure pump to be concentrated, so as to obtain 4 times of concentrated solution;
(3) Anaerobic fermentation: anaerobic sludge is added into an anaerobic fermentation reactor, concentrated solution is injected into the anaerobic fermentation reactor, the volume of the anaerobic sludge is 15 percent of the volume of the concentrated solution, and bacterial liquid (the number of viable bacteria is 1.2 multiplied by 10) of the sugar-producing zymophyte is added into the anaerobic fermentation reactor in an amount of 20 percent of the volume of the concentrated solution 8 cfu/L), adjusting the pH value in the anaerobic fermentation reactor to 8.0, fermenting at 40 ℃, and sealing and anaerobic fermentation for 40 hours;
(4) And (3) centrifuging: after the anaerobic fermentation product is centrifuged for 10 minutes at 5000 rpm, the supernatant is taken to obtain a high-concentration micromolecular carbon source, and the high-concentration micromolecular carbon source can be directly used as a denitrification liquid carbon source.
The COD concentration of the detected high-concentration micromolecular carbon source reaches 50 ten thousand mg/L.
Example 2
The embodiment provides a method for producing a denitrification carbon source for sewage treatment by using organic waste residues in the food industry, wherein the process flow is shown in a figure 1, and the specific operation steps are as follows:
(1) Pretreatment: crushing organic waste residues in the food industry under the extrusion action of 0.16MPa, adding hot water (83 ℃) which is 4 times of the mass of the organic waste residues, uniformly mixing, filtering the mixture through a 10-mesh and 20-mesh screen, controlling the particle size of the organic slurry to be below 0.25mm, and retaining about 46% of protein in the organic waste residues by filter residues;
(2) Reverse osmosis concentration: the organic slurry is conveyed to a DTRO system through a conveying belt by a high-pressure pump to be concentrated, so as to obtain 5 times concentrated solution;
(3) Anaerobic fermentation: anaerobic sludge is added into the anaerobic fermentation reactor, concentrated solution is injected into the anaerobic fermentation reactor, the volume of the anaerobic sludge is 20 percent of the volume of the concentrated solution, and the concentrated solution is added into the anaerobic fermentation reactor to produce the anaerobic fermentation reactorBacterial liquid of acetic acid sugar fermentation bacteria (viable count is 1.2X10) 8 cfu/L), regulating the pH value in the anaerobic fermentation reactor to 8.2, fermenting at 43 ℃, and sealing and anaerobic fermentation for 42h;
(4) And (3) centrifuging: and centrifuging the anaerobic fermentation product for 8 minutes at 6000 rpm, and taking supernatant to obtain a high-concentration micromolecular carbon source which can be directly used as a denitrification liquid carbon source.
The COD concentration of the detected high-concentration micromolecular carbon source reaches 53 ten thousand mg/L.
Example 3
The embodiment provides a method for producing a denitrification carbon source for sewage treatment by using organic waste residues in the food industry, wherein the process flow is shown in a figure 1, and the specific operation steps are as follows:
(1) Pretreatment: crushing organic waste residues in the food industry under the extrusion action of 0.14MPa, adding hot water (88 ℃) which is 3 times of the mass of the organic waste residues, uniformly mixing, filtering the mixture through a 10-mesh and 20-mesh screen, controlling the particle size of the organic slurry to be below 0.25mm, and retaining about 46% of protein in the organic waste residues by filter residues;
(2) Reverse osmosis concentration: the organic slurry is conveyed to a DTRO system through a conveying belt by a high-pressure pump to be concentrated, so as to obtain 5 times concentrated solution;
(3) Anaerobic fermentation: anaerobic sludge is added into an anaerobic fermentation reactor, concentrated solution is injected into the anaerobic fermentation reactor, the volume of the anaerobic sludge is 25 percent of the volume of the concentrated solution, and bacterial liquid (the number of viable bacteria is 1.2 multiplied by 10) of acetic acid sugar-producing zymophyte is added into 30 percent of the volume of the concentrated solution 8 cfu/L), adjusting the pH value in the anaerobic fermentation reactor to 8.5, fermenting at 38 ℃, and sealing and anaerobic fermentation for 48 hours;
(4) And (3) centrifuging: and centrifuging the anaerobic fermentation product for 12 minutes at 6000 rpm, and taking supernatant to obtain a high-concentration micromolecular carbon source which can be directly used as a denitrification liquid carbon source.
The COD concentration of the detected high-concentration micromolecular carbon source reaches 59 ten thousand mg/L.
Experimental example 1 anaerobic fermentation comparative experiment
To investigate the effect of adding a sugar-producing fermentation broth on the anaerobic fermentation effect, the concentration obtained in step (2) of example 1 was usedThe liquid is anaerobic fermentation substrate, and two identical batch reactors are arranged for comparison experiments. The effective volume of the reactor is 2L, the pH value is set to 8, and the reactor is kept in a homogeneous and constant-temperature state by a stirrer and a water bath kettle with the temperature of 40 ℃ in the fermentation process. 200mL of anaerobic sludge with the concentration of 5000mg/L and 1L of concentrated solution are added into a reactor, and bacterial liquid (the number of viable bacteria is 1.2X10) of acetic acid-producing sugar fermentation bacteria is added 8 cfu/L) was added to the reactor at 20% of the volume of the concentrate, and the reactor without adding the acetogenic sugar fermentor was used as a control group.
Samples were taken from the reactor every 12 hours (50 mL each) for analysis and fermentation was stopped while the components in the system remained essentially stable. COD, ammonia nitrogen, total carbohydrates, etc. were analyzed immediately after each sampling. After centrifugation of the fermentation broth at 50000 rpm for 10 minutes, the supernatant was filtered through a 0.45 μm filter, and the filtrate was analyzed to determine dissolved COD, carbohydrates and VFAs.
COD was determined by standard methods of GB 11914-89 "determination of chemical oxygen demand of Water quality" dichromate method.
The ammonia nitrogen is measured by adopting the standard method of HJ 535-2009 'Nahner reagent spectrophotometry for measuring ammonia nitrogen in water'.
Carbohydrates were measured using the anthrone reagent method and VFAs were analyzed using gas chromatography.
The acidification rate of the anaerobic fermentation was calculated according to the following formula:
wherein:
SCOD-the dissolved COD in the reactor after fermentation, g/L;
COD VFAs COD equivalent of VFAs in the reactor after fermentation, g/L.
From COD VFAs In terms of concentration, when adding acetogenic sugar fermentation bacteria for anaerobic fermentation, COD in the reactor after fermentation is finished VFAs 50.83 ten thousand g/L, which is 2.6 times that of the control group; the acidification rate of the reactor added with the acetogenic sugar fermentation bacteria for anaerobic fermentation is 2.4 times of that of the control group through calculation. From the following componentsThe result shows that the acetogenic sugar fermentation bacteria obviously improve the hydrolysis and acidification efficiency of the matrix.
Experimental example 2 Denitrification comparative experiment
In order to explore the influence of anaerobic fermentation liquid added with acetogenic sugar fermentation bacteria as a carbon source on denitrification performance, denitrification experiments are carried out on fermentation liquid which runs stably in the experimental example 1. For comparison, 3 sealed bottles were prepared, the carbon sources of which were blank, fermentation broth 1 (fermentation broth obtained by fermentation with the addition of sugar-producing fermentation bacteria), and fermentation broth 2 (fermentation broth obtained by fermentation without the addition of sugar-producing fermentation bacteria), respectively. Before the denitrification experiment starts, in order to ensure that the sludge activity is equivalent, the anaerobic sludge obtained from a sewage plant is domesticated by utilizing the carbon source to ensure that the denitrification rate is stabilized to be more than 90 percent, then the domesticated anaerobic sludge is panned by ultrapure water, the domesticated anaerobic sludge is placed in a sealed bottle, the volume of the anaerobic sludge is fixed to be 1.5L by the ultrapure water, and N is introduced 2 Removing dissolved oxygen from the solution, adding NaNO 3 And a carbon source (blank, broth 1, broth 2) such that NO 3 - The final mass concentrations of the N and the COD are (35+/-5) mg/L and (250+/-20) mg/L respectively, and the mass concentration of the sludge is (2000+/-100) mg/L. The pH of the reaction system is kept between 7.0 and 8.0. In the experimental process, a stirrer is adopted for continuous stirring and mixing, and the NO is analyzed by timing sampling 3 - -N、NO 2 - -N and COD.
Denitrification rate (V) of fermentation broth as carbon source DN ) The calculation formula of (2) is as follows:
V DN ＝d(NO x -N)
wherein:
V DN -denitrification rate, mg/(g·h);
x-sludge mass concentration (MLSS), g/L;
t-denitrification time, h;
NO x，in -NO at the very beginning of the reaction x Concentration, mg/L;
NO x，end(t) -atNO at t x Concentration, mg/L.
Denitrification ability (P) of fermentation broth as carbon source DN ) The calculation formula of (2) is as follows:
COD in -adding COD concentration of carbon source, mg/L;
COD end COD concentration at the end of denitrification, mg/L;
NO x，end -NO when the carbon source is used up by the microorganism x Concentration, mg/L.
The experimental data are shown in table 1.
TABLE 1 results of denitrification comparison experiments
| Index (I) | Blank space | Fermentation liquor 1 | Fermentation liquor 2 |
| COD in (mg/L) | 242 | 234 | 247 |
| COD end (mg/L) | 29 | 10 | 9.4 |
| NO x，in (mg/L) | 32.4 | 35 | 34.2 |
| NO x，end(t) (mg/L) | 16.5 | 2 | 9.1 |
| NO x，end (mg/L) | 10.3 | 0.3 | 4.9 |
| T(h) | 2 | 2 | 2 |
| V DN mg/(g·h) | 4.0 | 8.3 | 6.3 |
| P DN (mg/g) | 0.104 | 0.155 | 0.123 |
As shown in Table 1, the denitrification capability (P) of fermentation broth 1 cultured by adding sugar-producing fermentation bacteria DN ) And denitrification rate (V) DN ) Obviously higher than the blank, P of fermentation liquor 1 DN Compared with the fermentation liquor 2 without adding acetic acid sugar producing zymophyte, the fermentation liquor is higher by more than 20 percent, V DN Above 30%, addThe denitrification rate and denitrification potential of anaerobic fermentation liquid of the acetogenic sugar fermentation bacteria are greatly improved, the carbon source utilization rate is higher, and the denitrification capability is better.
It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. While still being apparent from variations or modifications that may be made by those skilled in the art are within the scope of the invention.
Claims (10)
1. The method for producing the denitrifying carbon source for sewage treatment by utilizing the organic waste residues in the food industry is characterized by comprising the following steps of:
(1) Pretreating organic waste residues in the food industry to prepare organic slurry;
(2) Performing reverse osmosis concentration treatment on the organic slurry to obtain concentrated solution;
(3) Anaerobic sludge is added into the anaerobic fermentation reactor, the concentrated solution is injected into the anaerobic fermentation reactor, and acetic acid sugar producing zymophyte is inoculated for anaerobic fermentation;
(4) And centrifuging the anaerobic fermentation product, and taking supernatant to obtain the denitrification carbon source for sewage treatment.
2. The method for producing a denitrified carbon source for sewage treatment by using organic waste residues in the food industry according to claim 1, wherein in the step (3), the sugar-producing zymophyte specifically selects a sugar-producing zymophyte (Saccharofermentans acetigenes) CGMCC 1.5064.
3. The method for producing denitrified carbon sources used in sewage treatment in food industry according to claim 1, characterized in that in step (3), bacterial liquid of sugar-producing fermentation bacteria is added in an amount of 20% -30% of the volume of the concentrated solution, and the viable count of the bacterial liquid of the sugar-producing fermentation bacteria is 1.2×10 8 ～1.3×10 8 cfu/L。
4. The method for producing a denitrified carbon source for sewage treatment by using organic waste residues in the food industry according to claim 1, wherein in the step (3), the amount of the anaerobic sludge is 15% -25% of the volume of the concentrated solution; the VSS/TSS of the anaerobic sludge is larger than 0.7, and the sludge concentration is 5000+/-100 mg/L.
5. The method for producing a denitrified carbon source for sewage treatment by using organic waste residues in food industry according to claim 1, wherein in the step (3), the anaerobic fermentation conditions are as follows: the pH value is 8.0-8.5, the temperature is 35-45 ℃ and the time is 36-48 h.
6. The method for producing a denitrified carbon source for sewage treatment by using organic waste residues in food industry according to claim 1, wherein in the step (1), the pretreatment includes: crushing the organic waste residues in the food industry, adding hot water, uniformly mixing, and filtering to obtain the organic slurry, wherein the pressure during crushing is controlled to be 0.13-0.15 Mpa; the mass of the added hot water is 2 to 5 times of the mass of the organic waste residue, and the temperature of the hot water is 80 to 90 ℃; the filtering is carried out for 1 to 3 times by using a screen mesh with 10 to 50 meshes.
7. The method for producing a denitrified carbon source for sewage treatment by using organic waste residues in the food industry according to claim 1, wherein in the step (2), a disc-tube type reverse osmosis membrane is adopted for the reverse osmosis concentration treatment; concentrating the organic slurry by 3-5 times through the reverse osmosis concentration treatment.
8. The method for producing a denitrified carbon source for sewage treatment by using organic waste residues in food industry according to claim 1, wherein in the step (4), the centrifugation condition is: 4000-6000 rpm for 8-12 min.
9. A denitrification carbon source for sewage treatment produced by the method of any one of claims 1 to 8.
10. A sewage treatment method comprising denitrification treatment of sewage, characterized in that a carbon source used in the denitrification treatment is produced by the method according to any one of claims 1 to 8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310125812.8A CN116251819A (en) | 2023-02-01 | 2023-02-01 | Method for producing denitrifying carbon source for sewage treatment by utilizing organic waste residues in food industry |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310125812.8A CN116251819A (en) | 2023-02-01 | 2023-02-01 | Method for producing denitrifying carbon source for sewage treatment by utilizing organic waste residues in food industry |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116251819A true CN116251819A (en) | 2023-06-13 |
Family
ID=86685862
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310125812.8A Pending CN116251819A (en) | 2023-02-01 | 2023-02-01 | Method for producing denitrifying carbon source for sewage treatment by utilizing organic waste residues in food industry |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116251819A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117361748A (en) * | 2023-11-28 | 2024-01-09 | 山东绿邦生物科技有限公司 | Carbon source for wastewater denitrification treatment, and preparation method and application thereof |
-
2023
- 2023-02-01 CN CN202310125812.8A patent/CN116251819A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117361748A (en) * | 2023-11-28 | 2024-01-09 | 山东绿邦生物科技有限公司 | Carbon source for wastewater denitrification treatment, and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Yan et al. | Anaerobic digestion of cheese whey using up-flow anaerobic sludge blanket reactor | |
| Sans et al. | Volatile fatty acids production by mesophilic fermentation of mechanically-sorted urban organic wastes in a plug-flow reactor | |
| Jia et al. | Yields of biomass and extracellular polymers in four anaerobic sludges | |
| CN110255712B (en) | Preparation method of denitrification carbon source | |
| CN113788537A (en) | Low-C/N-ratio sewage biological denitrification composite carbon source and preparation method thereof | |
| CN113264588B (en) | Composite carbon source for sewage treatment | |
| CN112174337B (en) | Application of kitchen waste fermentation liquor in sewage treatment | |
| CN108298701B (en) | Low-biodegradability fermentation wastewater treatment method after anaerobic treatment | |
| CN115385456B (en) | Efficient denitrification sewage treatment agent and preparation method thereof | |
| KR100352811B1 (en) | Methods for rapid digestion of food wastes and for methane production using three-stage methane fermentaion system | |
| CN116251819A (en) | Method for producing denitrifying carbon source for sewage treatment by utilizing organic waste residues in food industry | |
| Hafez et al. | Flax retting wastewater Part 1: Anaerobic treatment by using UASB reactor | |
| CN113307377A (en) | Method for treating fermentation exhaust gas and wastewater by coupling active microalgae | |
| Borja et al. | Kinetic behaviour of waste tyre rubber as microorganism support in an anaerobic digester treating cane molasses distillery slops | |
| CN113754181B (en) | Polyester wastewater treatment method and device | |
| CN116083495A (en) | Method for relieving inhibition of anaerobic digestion ammonia nitrogen by utilizing saccharomycetes | |
| KR100417761B1 (en) | Method for carbon source of biological denitrification using distillery wastewater | |
| Chen et al. | Two-stage air stripping combined with hydrolysis acidification process for coal gasification wastewater pretreatment | |
| Yu et al. | Anaerobic acidification of a synthetic wastewater in batch reactors at 55 C | |
| Manilal et al. | Anaerobic digestion of cassava starch factory effluent | |
| CN111334533A (en) | Method for producing volatile fatty acid by promoting anaerobic fermentation of office waste paper and sludge by cellulase | |
| CN112522151A (en) | Preparation method and application of composite methanogen | |
| CN111977893A (en) | Biochemical treatment method for wastewater generated in production of hydrogen peroxide by anthraquinone process based on composite microbial inoculum | |
| Fubao et al. | Water-recycled cassava bioethanol production integrated with two-stage UASB treatment | |
| US20230111148A1 (en) | Efficient multi-dimensional carbon source and method for preparing the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |