CN111781185A - Multi-fluorescence channel detection system for real-time fluorescence quantitative PCR - Google Patents
Multi-fluorescence channel detection system for real-time fluorescence quantitative PCR Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 36
- 238000003753 real-time PCR Methods 0.000 title claims abstract description 15
- 230000008878 coupling Effects 0.000 claims description 36
- 238000010168 coupling process Methods 0.000 claims description 36
- 238000005859 coupling reaction Methods 0.000 claims description 36
- 239000013307 optical fiber Substances 0.000 claims description 29
- 230000005284 excitation Effects 0.000 claims description 21
- 239000000835 fiber Substances 0.000 claims description 17
- 238000005187 foaming Methods 0.000 claims description 12
- 239000004816 latex Substances 0.000 claims description 12
- 229920000126 latex Polymers 0.000 claims description 12
- 238000003752 polymerase chain reaction Methods 0.000 abstract description 5
- 230000003287 optical effect Effects 0.000 description 17
- 230000001681 protective effect Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 229920001821 foam rubber Polymers 0.000 description 1
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- G—PHYSICS
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- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B7/00—Mountings, adjusting means, or light-tight connections, for optical elements
- G02B7/006—Filter holders
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B7/00—Mountings, adjusting means, or light-tight connections, for optical elements
- G02B7/02—Mountings, adjusting means, or light-tight connections, for optical elements for lenses
- G02B7/021—Mountings, adjusting means, or light-tight connections, for optical elements for lenses for more than one lens
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B7/00—Mountings, adjusting means, or light-tight connections, for optical elements
- G02B7/02—Mountings, adjusting means, or light-tight connections, for optical elements for lenses
- G02B7/023—Mountings, adjusting means, or light-tight connections, for optical elements for lenses permitting adjustment
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Abstract
The invention discloses a multi-fluorescence-channel detection system for real-time fluorescence quantitative PCR (polymerase chain reaction), which comprises a first box body and a second box body, wherein the first box body and the second box body are both of rectangular box body structures with one side opened, and one side of the first box body is hinged with one side of the second box body.
Description
Technical Field
The invention relates to a detection system, in particular to a multi-fluorescence channel detection system for real-time fluorescence quantitative PCR.
Background
Publication No.: CN105675574B discloses a multi-fluorescence-channel detection system for real-time fluorescence quantitative PCR, and we find that the optical fiber coupling lens, the excitation filter, the collimating lens and the like of the device do not have a protection structure in application, so that the device is easy to collide and damage on one hand, and on the other hand, the device is easy to be influenced by external light to cause inaccurate detection.
Disclosure of Invention
The invention aims to provide a multi-fluorescence-channel detection system for real-time fluorescence quantitative PCR, which can be additionally provided with a first box body, a second box body, a light-emitting auxiliary mechanism and a photoelectric sensing protection device on the basis of the prior art, wherein the first box body and the second box body can provide first-stage protection for a first optical fiber coupling lens, a first excitation optical filter, a first collimation lens and the like, and the lenses and the optical filters are prevented from being easily collided and damaged.
In order to achieve the purpose, the invention is realized by the following technical scheme:
a multi-fluorescence-channel detection system for real-time fluorescence quantitative PCR comprises a first box body and a second box body, wherein the first box body and the second box body are rectangular box body structures with one side opened, one side of the first box body is hinged with one side of the second box body, the first box body and the second box body can be buckled to form a closed rectangular structure, a light emitting auxiliary mechanism is arranged inside the first box body and comprises a first light shading pipe, the first light shading pipe is a tubular shell structure with one end sealed, an internal thread is arranged on the inner wall of the first light shading pipe, a light source is arranged at the bottom of the inner side of the first light shading pipe, a first upper positioning ring, a first middle positioning ring and a first lower positioning ring are arranged inside the first light shading pipe, the first lower positioning ring is positioned above the light source and is positioned above the first lower positioning ring, the first upper positioning ring is positioned above the first middle positioning ring, the periphery of the first upper positioning ring, the periphery of the first middle positioning ring and the periphery of the first lower positioning ring are provided with external threads, the first upper positioning ring, the first middle positioning ring and the first lower positioning ring are in threaded connection with the first shading pipe, the inner side of the first upper positioning ring is connected with the first optical fiber coupling lens, the inner side of the first middle positioning ring is connected with the first excitation optical filter, the inner side of the first lower positioning ring is connected with the first collimating lens, the interior of the second box body is provided with the photoelectric sensing protection device, the photoelectric sensing protection device comprises a second shading pipe, the second shading pipe is of a tubular shell structure with one sealed end, the inner wall of the second shading pipe is provided with internal threads, the bottom of the inner side of the second shading pipe is provided with a photoelectric sensor, the interior of the second shading pipe is provided with the second upper positioning ring, the second middle positioning ring and the second lower positioning ring, the second lower positioning ring is positioned above the photoelectric sensor, the locating ring is located the top of locating ring in the second on the second, and the external screw thread is all seted up to the periphery of locating ring under locating ring, second in the second, locating ring, the second in the second all with second shading pipe threaded connection, the inboard second optical fiber coupling lens of connecting of locating ring on the second, the inboard detection light filter of connecting of locating ring in the second, the inboard convergent lens of connecting ring under the second.
In order to further realize the aim of the invention, the following technical scheme can be adopted, wherein the bottom of the first light shading pipe is connected with a first supporting spring and a first supporting foaming latex block, one side of the first supporting foaming latex block is connected with the inner wall of the first box body, one end of the first supporting spring is connected with the inside of the first box body, the bottom of the second light shading pipe is connected with a second supporting spring and a second supporting foaming latex block, one end of the second supporting spring is connected with the inner wall of the second box body, and one side of the second supporting foaming latex block is connected with the inside of the second box body.
A first elastic buffer device is arranged between the first light shielding pipe and the first box body, the first elastic buffer device comprises a first inner ring and a first outer ring, the first inner ring is sleeved on the first light shielding pipe, the outer side of the first outer ring is connected with the inner wall of the first box body, a plurality of first horizontal springs are arranged between the inner wall of the first inner ring and the outer side of the first outer ring, one end of each first horizontal spring is connected with the first inner ring, the other end of each first horizontal spring is connected with the first outer ring, a second elastic buffer device is arranged between the second light shielding pipe and the second box body, the second elastic buffer device comprises a second inner ring and a second outer ring, the second inner ring is sleeved on the second light shielding pipe, the outer side of the second outer ring is connected with the inner wall of the second box body, a plurality of second horizontal springs are arranged between the inner wall of the second inner ring and the outer side of the second outer ring, and one end of each second horizontal spring is connected with the second inner ring, the other end of the second horizontal spring is connected with the second outer ring.
The upper part of the first box body is provided with a first through groove, and the upper part of the second box body is provided with a second through groove.
The invention has the advantages that: the first box body, the second box body, the light-emitting auxiliary mechanism and the photoelectric sensing protection device are additionally arranged on the basis of the prior art, the first box body and the second box body can provide first-level protection for the first optical fiber coupling lens, the first excitation optical filter, the first collimating lens and the like, and the lenses and the optical filter are prevented from being easily collided and damaged. Meanwhile, the first shading tube can provide protection for the first optical fiber coupling lens inside, the first excitation light filter, the first collimating lens and the like, the influence of external optical fibers on the detection of the first optical fiber coupling lens, the first excitation light filter, the first collimating lens and the like is avoided, the height of the first optical fiber coupling lens can be conveniently adjusted by the first upper positioning ring and the first shading tube in a threaded connection mode, the height of the first excitation light filter can be conveniently adjusted by the first middle positioning ring and the first shading tube in a threaded connection mode, the height of the first collimating lens can be conveniently adjusted by the first lower positioning ring and the first shading tube in a threaded connection mode, the first upper positioning ring, the first middle positioning ring and the first lower positioning ring are combined, the first optical fiber coupling lens can be conveniently adjusted, and the relative position between the first excitation light filter and the first collimating lens can be conveniently adjusted. The second shading tube can be the inside second optical fiber coupling lens, detection light filter and second collimating lens etc. provide the protection, avoid external optic fibre to second optical fiber coupling lens, the detection of detection light filter and second collimating lens etc. causes the influence, wherein holding ring and second shading tube threaded connection can conveniently adjust the height of second optical fiber coupling lens on the second, holding ring and second shading tube threaded connection can conveniently adjust the height of detection light filter in the second, holding ring and second shading tube threaded connection can conveniently adjust the height of second collimating lens under the second, holding ring on the second, holding ring and second holding ring three combine under holding ring and the second in the second, can conveniently adjust second optical fiber coupling lens, detect the relative position between light filter and the second collimating lens.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic view of the present invention;
FIG. 2 is a second schematic structural diagram of the present invention;
FIG. 3 is a third schematic structural diagram of the present invention;
fig. 4 is a schematic structural view of the first casing 50 and the second casing 51 closed;
FIG. 5 is an enlarged view of the structure of FIG. 2 from direction A;
FIG. 6 is an enlarged view of the structure of FIG. 2 from the direction B;
FIG. 7 is a fourth schematic view of the present invention.
Detailed Description
Preferred embodiments of the present invention will be described below with reference to the accompanying drawings.
Example 1:
a multi-fluorescence-channel detection system for real-time fluorescence quantitative PCR (polymerase chain reaction), as shown in figures 1-7, comprises a first box 50 and a second box 51, wherein the first box 50 and the second box 51 are both of a cuboid box structure with an opening at one side, one side of the first box 50 is hinged with one side of the second box 51, the first box 50 and the second box 51 can be buckled to form a closed cuboid structure, a light-emitting auxiliary mechanism is arranged in the first box 50, the light-emitting auxiliary mechanism comprises a first light shielding pipe 8, the first light shielding pipe 8 is of a tubular shell structure with one end sealed, the inner wall of the first light shielding pipe 8 is provided with an internal thread, the bottom of the inner side of the first light shielding pipe 8 is provided with a light source 7, the first upper positioning ring 1, the first middle positioning ring 4 and the first lower positioning ring 6 are arranged in the first light shielding pipe 8, the first lower positioning ring 6 is positioned above the light source 7, the first middle positioning ring 4 is positioned above the first lower positioning ring 6, the first upper positioning ring 1 is positioned above the first middle positioning ring 4, the peripheries of the first upper positioning ring 1, the first middle positioning ring 4 and the first lower positioning ring 6 are all provided with external threads, the first upper positioning ring 1, the first middle positioning ring 4 and the first lower positioning ring 6 are all in threaded connection with the first shading tube 8, the inner side of the first upper positioning ring 1 is connected with the first optical fiber coupling lens 2, the inner side of the first middle positioning ring 4 is connected with the first excitation optical filter 3, the inner side of the first lower positioning ring 6 is connected with the first collimating lens 5, the photoelectric sensing protective device is arranged in the second box body 51 and comprises a second shading tube 38, the second shading tube 38 is a tubular shell structure with one end sealed, the inner wall of the second shading tube 38 is provided with internal threads, the bottom of the inner side of the second shading tube 38 is provided with a photoelectric sensor 37, a second upper positioning ring 31, a second middle positioning ring 34 and a second lower positioning ring 36 are arranged in the second light shading tube 38, the second lower positioning ring 36 is positioned above the photoelectric sensor 37, the second middle positioning ring 34 is positioned above the second lower positioning ring 36, the second upper positioning ring 31 is positioned above the second middle positioning ring 34, the peripheries of the second upper positioning ring 31, the second middle positioning ring 34 and the second lower positioning ring 36 are all provided with external threads, the second upper positioning ring 31, the second middle positioning ring 34 and the second lower positioning ring 36 are all in threaded connection with the second light shading tube 38, the inner side of the second upper positioning ring 31 is connected with the second fiber coupling lens 32, the inner side of the second middle positioning ring 34 is connected with the detection optical filter 33, and the inner side of the second lower positioning ring 36 is connected with the convergent lens 35.
The invention is additionally provided with the first box body 50, the second box body 51, the light-emitting auxiliary mechanism and the photoelectric sensing protection device on the basis of the prior art, the first box body 50 and the second box body 51 can provide first-level protection for the first optical fiber coupling lens 2, the first excitation optical filter 3, the first collimating lens 5 and the like, and the lenses and the optical filters are prevented from being easily collided and damaged. Meanwhile, the first light shielding pipe 8 can provide protection for the first fiber coupling lens 2, the first excitation filter 3, the first collimating lens 5 and the like inside, so as to avoid the influence of external fibers on the detection of the first fiber coupling lens 2, the first excitation filter 3, the first collimating lens 5 and the like, wherein first holding ring 1 and first 8 threaded connections of shading pipe can conveniently adjust the height of first fiber coupling lens 2, holding ring 4 and first 8 threaded connections of shading pipe can conveniently adjust the height of first excitation light filter 3 in first, first holding ring 6 and first 8 threaded connections of shading pipe can conveniently adjust the height of first collimation lens 5 down, first holding ring 1 of going up, holding ring 4 and first 6 three of holding ring combine down, can conveniently adjust first fiber coupling lens 2, the relative position between first excitation light filter 3 and the first collimation lens 5. The second light shielding pipe 38 can provide protection for the second fiber coupling lens 32, the detection filter 33, the second collimating lens 35, etc. inside, so as to avoid the influence of the external fiber on the detection of the second fiber coupling lens 32, the detection filter 33, the second collimating lens 35, etc., the height of the second optical fiber coupling lens 32 can be conveniently adjusted by the threaded connection of the second upper positioning ring 31 and the second light shielding pipe 38, the height of the detection optical filter 33 can be conveniently adjusted by the threaded connection of the second middle positioning ring 34 and the second light shielding pipe 38, the height of the second collimating lens 35 can be conveniently adjusted by the threaded connection of the second lower positioning ring 36 and the second light shielding pipe 38, the second upper positioning ring 31, the second middle positioning ring 34 and the second lower positioning ring 36 are combined, and the relative positions of the second optical fiber coupling lens 32, the detection optical filter 33 and the second collimating lens 35 can be conveniently adjusted.
Material selection and feasibility analysis of specific implementation of application range: the practical manufactured sample is implemented by taking the specification attached drawing as a drawing according to the proportion and the matching mode of each part in the specification attached drawing, the connection is a common connection mode such as a connection of a strong adhesive, a welding, riveting and a flange connection and an integral forming type connection, and the thickness and the strength of a corresponding connection mode connection point can be selected without creativity according to the requirement of practical connection strength during practical manufacturing. The application is disclosed in the publication number: CN105675574B patent, wherein the publication number: the CN105675574B patent further includes a light source, a collimating lens, an excitation filter, an optical fiber coupling lens, an excitation optical fiber, a test tube, a thermometer, a reaction solution, a detection optical fiber, a detection filter, a converging lens, a photoelectric sensor, a dichroic mirror, an optical fiber disc, a turntable, an optical fiber, and a driving motor. Publication No.: the light source, the first collimating lens, the excitation filter, the fiber coupling lens of the CN105675574B patent are installed inside the first light shielding tube 8, the detection filter, the converging lens, the photoelectric sensor, and the second collimating lens are installed inside the second light shielding tube 38. The first fiber coupling lens 2 and the second fiber coupling lens 32 are matched with the optical fiber in a way of adopting the following publication numbers: CN105675574B patent publication.
Example 2:
a multi-fluorescence-channel detection system for real-time fluorescence quantitative PCR (polymerase chain reaction), as shown in figures 1-7, comprises a first box 50 and a second box 51, wherein the first box 50 and the second box 51 are both of a cuboid box structure with an opening at one side, one side of the first box 50 is hinged with one side of the second box 51, the first box 50 and the second box 51 can be buckled to form a closed cuboid structure, a light-emitting auxiliary mechanism is arranged in the first box 50, the light-emitting auxiliary mechanism comprises a first light shielding pipe 8, the first light shielding pipe 8 is of a tubular shell structure with one end sealed, the inner wall of the first light shielding pipe 8 is provided with an internal thread, the bottom of the inner side of the first light shielding pipe 8 is provided with a light source 7, the first upper positioning ring 1, the first middle positioning ring 4 and the first lower positioning ring 6 are arranged in the first light shielding pipe 8, the first lower positioning ring 6 is positioned above the light source 7, the first middle positioning ring 4 is positioned above the first lower positioning ring 6, the first upper positioning ring 1 is positioned above the first middle positioning ring 4, the peripheries of the first upper positioning ring 1, the first middle positioning ring 4 and the first lower positioning ring 6 are all provided with external threads, the first upper positioning ring 1, the first middle positioning ring 4 and the first lower positioning ring 6 are all in threaded connection with the first shading tube 8, the inner side of the first upper positioning ring 1 is connected with the first optical fiber coupling lens 2, the inner side of the first middle positioning ring 4 is connected with the first excitation optical filter 3, the inner side of the first lower positioning ring 6 is connected with the first collimating lens 5, the photoelectric sensing protective device is arranged in the second box body 51 and comprises a second shading tube 38, the second shading tube 38 is a tubular shell structure with one end sealed, the inner wall of the second shading tube 38 is provided with internal threads, the bottom of the inner side of the second shading tube 38 is provided with a photoelectric sensor 37, a second upper positioning ring 31, a second middle positioning ring 34 and a second lower positioning ring 36 are arranged in the second light shading tube 38, the second lower positioning ring 36 is positioned above the photoelectric sensor 37, the second middle positioning ring 34 is positioned above the second lower positioning ring 36, the second upper positioning ring 31 is positioned above the second middle positioning ring 34, the peripheries of the second upper positioning ring 31, the second middle positioning ring 34 and the second lower positioning ring 36 are all provided with external threads, the second upper positioning ring 31, the second middle positioning ring 34 and the second lower positioning ring 36 are all in threaded connection with the second light shading tube 38, the inner side of the second upper positioning ring 31 is connected with the second fiber coupling lens 32, the inner side of the second middle positioning ring 34 is connected with the detection optical filter 33, and the inner side of the second lower positioning ring 36 is connected with the convergent lens 35. First supporting spring 9 and the first internal connection that supports the foaming latex 10 soon are connected to the bottom of first shading pipe 8, and one side that first support foaming latex 10 soon is connected with the internal wall of first box 50, the one end of first supporting spring 9 and the internal connection of first box 50, second supporting spring 39 and the second support foaming latex 40 soon are connected to the bottom of second shading pipe 38, and the one end of second supporting spring 39 and the internal connection of second box 51, one side that the second supports the foaming latex 40 soon and the internal connection of second box 51.
A supporting foamed emulsion block 10 and a first supporting spring 9 of the present invention can provide a buffer for the bottom of the first light shielding tube 8. The second supporting spring 39 and the second supporting latex foam block 40 of the present invention can provide a buffer for the bottom of the second light shielding tube 38.
Example 3:
a multi-fluorescence-channel detection system for real-time fluorescence quantitative PCR (polymerase chain reaction), as shown in figures 1-7, comprises a first box 50 and a second box 51, wherein the first box 50 and the second box 51 are both of a cuboid box structure with an opening at one side, one side of the first box 50 is hinged with one side of the second box 51, the first box 50 and the second box 51 can be buckled to form a closed cuboid structure, a light-emitting auxiliary mechanism is arranged in the first box 50, the light-emitting auxiliary mechanism comprises a first light shielding pipe 8, the first light shielding pipe 8 is of a tubular shell structure with one end sealed, the inner wall of the first light shielding pipe 8 is provided with an internal thread, the bottom of the inner side of the first light shielding pipe 8 is provided with a light source 7, the first upper positioning ring 1, the first middle positioning ring 4 and the first lower positioning ring 6 are arranged in the first light shielding pipe 8, the first lower positioning ring 6 is positioned above the light source 7, the first middle positioning ring 4 is positioned above the first lower positioning ring 6, the first upper positioning ring 1 is positioned above the first middle positioning ring 4, the peripheries of the first upper positioning ring 1, the first middle positioning ring 4 and the first lower positioning ring 6 are all provided with external threads, the first upper positioning ring 1, the first middle positioning ring 4 and the first lower positioning ring 6 are all in threaded connection with the first shading tube 8, the inner side of the first upper positioning ring 1 is connected with the first optical fiber coupling lens 2, the inner side of the first middle positioning ring 4 is connected with the first excitation optical filter 3, the inner side of the first lower positioning ring 6 is connected with the first collimating lens 5, the photoelectric sensing protective device is arranged in the second box body 51 and comprises a second shading tube 38, the second shading tube 38 is a tubular shell structure with one end sealed, the inner wall of the second shading tube 38 is provided with internal threads, the bottom of the inner side of the second shading tube 38 is provided with a photoelectric sensor 37, a second upper positioning ring 31, a second middle positioning ring 34 and a second lower positioning ring 36 are arranged in the second light shading tube 38, the second lower positioning ring 36 is positioned above the photoelectric sensor 37, the second middle positioning ring 34 is positioned above the second lower positioning ring 36, the second upper positioning ring 31 is positioned above the second middle positioning ring 34, the peripheries of the second upper positioning ring 31, the second middle positioning ring 34 and the second lower positioning ring 36 are all provided with external threads, the second upper positioning ring 31, the second middle positioning ring 34 and the second lower positioning ring 36 are all in threaded connection with the second light shading tube 38, the inner side of the second upper positioning ring 31 is connected with the second fiber coupling lens 32, the inner side of the second middle positioning ring 34 is connected with the detection optical filter 33, and the inner side of the second lower positioning ring 36 is connected with the convergent lens 35. A first elastic buffer device is arranged between the first light shielding pipe 8 and the first box 50, the first elastic buffer device comprises a first inner ring 11 and a first outer ring 13, the first inner ring 11 is sleeved on the first light shielding pipe 8, the outer side of the first outer ring 13 is connected with the inner wall of the first box 50, a plurality of first horizontal springs 12 are arranged between the inner wall of the first inner ring 11 and the outer side of the first outer ring 13, one end of each first horizontal spring 12 is connected with the first inner ring 11, the other end of each first horizontal spring 12 is connected with the first outer ring 13, a second elastic buffer device is arranged between the second light shielding pipe 38 and the second box 51, the second elastic buffer device comprises a second inner ring 21 and a second outer ring 23, the second inner ring 21 is sleeved on the second light shielding pipe 38, the outer side of the second outer ring 23 is connected with the inner wall of the second box 51, and a plurality of second horizontal springs 22 are arranged between the inner wall of the second inner ring 21 and the outer side of the second outer ring 23, one end of the second horizontal spring 22 is connected to the second inner ring 21, and the other end of the second horizontal spring 22 is connected to the second outer ring 23.
The first elastic buffer device of the present invention can provide buffer for the first light shielding tube 8 in the horizontal direction, and the plurality of first horizontal springs 12 are all in a compressed state. The second elastic buffer device of the present invention can provide buffer for the second light shielding pipe 38 in the horizontal direction, and the plurality of second horizontal springs 22 are all in a compressed state.
Example 4:
a multi-fluorescence-channel detection system for real-time fluorescence quantitative PCR (polymerase chain reaction), as shown in figures 1-7, comprises a first box 50 and a second box 51, wherein the first box 50 and the second box 51 are both of a cuboid box structure with an opening at one side, one side of the first box 50 is hinged with one side of the second box 51, the first box 50 and the second box 51 can be buckled to form a closed cuboid structure, a light-emitting auxiliary mechanism is arranged in the first box 50, the light-emitting auxiliary mechanism comprises a first light shielding pipe 8, the first light shielding pipe 8 is of a tubular shell structure with one end sealed, the inner wall of the first light shielding pipe 8 is provided with an internal thread, the bottom of the inner side of the first light shielding pipe 8 is provided with a light source 7, the first upper positioning ring 1, the first middle positioning ring 4 and the first lower positioning ring 6 are arranged in the first light shielding pipe 8, the first lower positioning ring 6 is positioned above the light source 7, the first middle positioning ring 4 is positioned above the first lower positioning ring 6, the first upper positioning ring 1 is positioned above the first middle positioning ring 4, the peripheries of the first upper positioning ring 1, the first middle positioning ring 4 and the first lower positioning ring 6 are all provided with external threads, the first upper positioning ring 1, the first middle positioning ring 4 and the first lower positioning ring 6 are all in threaded connection with the first shading tube 8, the inner side of the first upper positioning ring 1 is connected with the first optical fiber coupling lens 2, the inner side of the first middle positioning ring 4 is connected with the first excitation optical filter 3, the inner side of the first lower positioning ring 6 is connected with the first collimating lens 5, the photoelectric sensing protective device is arranged in the second box body 51 and comprises a second shading tube 38, the second shading tube 38 is a tubular shell structure with one end sealed, the inner wall of the second shading tube 38 is provided with internal threads, the bottom of the inner side of the second shading tube 38 is provided with a photoelectric sensor 37, a second upper positioning ring 31, a second middle positioning ring 34 and a second lower positioning ring 36 are arranged in the second light shading tube 38, the second lower positioning ring 36 is positioned above the photoelectric sensor 37, the second middle positioning ring 34 is positioned above the second lower positioning ring 36, the second upper positioning ring 31 is positioned above the second middle positioning ring 34, the peripheries of the second upper positioning ring 31, the second middle positioning ring 34 and the second lower positioning ring 36 are all provided with external threads, the second upper positioning ring 31, the second middle positioning ring 34 and the second lower positioning ring 36 are all in threaded connection with the second light shading tube 38, the inner side of the second upper positioning ring 31 is connected with the second fiber coupling lens 32, the inner side of the second middle positioning ring 34 is connected with the detection optical filter 33, and the inner side of the second lower positioning ring 36 is connected with the convergent lens 35. The upper part of the first box 50 is provided with a first through groove 52, and the upper part of the second box 51 is provided with a second through groove 53.
The first through-grooves 52 and the second through-grooves 53 of the present invention may facilitate the passage of the optical fibers through the first housing 50 and the second housing 51, respectively.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention.
Claims (4)
1. A many fluorescence channel detecting system for real-time fluorescence quantitative PCR which characterized in that: the LED lamp box comprises a first box body (50) and a second box body (51), wherein the first box body (50) and the second box body (51) are rectangular box body structures with one side opened, one side of the first box body (50) is hinged with one side of the second box body (51), the first box body (50) and the second box body (51) can be buckled to form a closed rectangular structure, a light-emitting auxiliary mechanism is arranged inside the first box body (50), the light-emitting auxiliary mechanism comprises a first shading tube (8), the first shading tube (8) is a tubular shell structure with one sealed end, an inner thread is formed in the inner wall of the first shading tube (8), a light source (7) is arranged at the bottom of the inner side of the first shading tube (8), a first upper positioning ring (1), a first middle positioning ring (4) and a first lower positioning ring (6) are arranged inside the first shading tube (8), and the first lower positioning ring (6) is positioned above the light source (7), the first middle positioning ring (4) is positioned above the first lower positioning ring (6), the first upper positioning ring (1) is positioned above the first middle positioning ring (4), the peripheries of the first upper positioning ring (1), the first middle positioning ring (4) and the first lower positioning ring (6) are provided with external threads, the first upper positioning ring (1), the first middle positioning ring (4) and the first lower positioning ring (6) are in threaded connection with the first shading tube (8), the inner side of the first upper positioning ring (1) is connected with the first fiber coupling lens (2), the inner side of the first middle positioning ring (4) is connected with the first excitation filter (3), the inner side of the first lower positioning ring (6) is connected with the first collimating lens (5), the photoelectric sensing protection device is arranged in the second box body (51), the photoelectric sensing protection device comprises a second shading tube (38), and the second shading tube (38) is of a tubular shell structure with one end sealed, an inner thread is formed on the inner wall of the second light shading pipe (38), a photoelectric sensor (37) is arranged at the bottom of the inner side of the second light shading pipe (38), a second upper positioning ring (31), a second middle positioning ring (34) and a second lower positioning ring (36) are arranged inside the second light shading pipe (38), the second lower positioning ring (36) is positioned above the photoelectric sensor (37), the second middle positioning ring (34) is positioned above the second lower positioning ring (36), the second upper positioning ring (31) is positioned above the second middle positioning ring (34), outer threads are formed on the peripheries of the second upper positioning ring (31), the second middle positioning ring (34) and the second lower positioning ring (36) and are in threaded connection with the second light shading pipe (38), a second optical fiber coupling lens (32) is connected to the inner side of the second upper positioning ring (31), the inner side of the second middle positioning ring (34) is connected with a detection filter (33), and the inner side of the second lower positioning ring (36) is connected with a converging lens (35).
2. The multiple fluorescence channel detection system for real-time fluorescent quantitative PCR as claimed in claim 1, wherein: first supporting spring (9) and first support foaming latex are connected to the bottom of first shading pipe (8) and are fast (10), and one side of first support foaming latex (10) and the interior wall connection of first box (50), the one end of first supporting spring (9) and the interior connection of first box (50), second supporting spring (39) and second support foaming latex are fast (40) are connected to the bottom of second shading pipe (38), and the one end of second supporting spring (39) and the interior wall connection of second box (51), and the one side of second support foaming latex (40) and the interior connection of second box (51) are fast.
3. The multiple fluorescence channel detection system for real-time fluorescent quantitative PCR as claimed in claim 1, wherein: a first elastic buffer device is arranged between the first light shading pipe (8) and the first box body (50), the first elastic buffer device comprises a first inner ring (11) and a first outer ring (13), the first inner ring (11) is sleeved on the first light shading pipe (8), the outer side of the first outer ring (13) is connected with the inner wall of the first box body (50), a plurality of first horizontal springs (12) are arranged between the inner wall of the first inner ring (11) and the outer side of the first outer ring (13), one end of each first horizontal spring (12) is connected with the first inner ring (11), the other end of each first horizontal spring (12) is connected with the first outer ring (13), a second elastic buffer device is arranged between the second light shading pipe (38) and the second box body (51), the second elastic buffer device comprises a second inner ring (21) and a second outer ring (23), and the second inner ring (21) is sleeved on the second light shading pipe (38), the outer side of the second outer ring (23) is connected with the inner wall of the second box body (51), a plurality of second horizontal springs (22) are arranged between the inner wall of the second inner ring (21) and the outer side of the second outer ring (23), one end of each second horizontal spring (22) is connected with the second inner ring (21), and the other two ends of each second horizontal spring (22) are connected with the second outer ring (23).
4. The multiple fluorescence channel detection system for real-time fluorescent quantitative PCR as claimed in claim 1, wherein: the upper part of the first box body (50) is provided with a first through groove (52), and the upper part of the second box body (51) is provided with a second through groove (53).
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| CN202010806289.1A CN111781185A (en) | 2020-08-12 | 2020-08-12 | Multi-fluorescence channel detection system for real-time fluorescence quantitative PCR |
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| CN202010806289.1A CN111781185A (en) | 2020-08-12 | 2020-08-12 | Multi-fluorescence channel detection system for real-time fluorescence quantitative PCR |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0943132A (en) * | 1995-07-27 | 1997-02-14 | Fujitsu Ltd | Particle measuring instrument |
| CN105675574A (en) * | 2016-03-17 | 2016-06-15 | 苏州天隆生物科技有限公司 | Multi-fluorescence channel detection system for real-time fluorescence quantitative PCR |
| CN108593554A (en) * | 2018-05-24 | 2018-09-28 | 大连民族大学 | It is a kind of can multidimensional adjust laser induced fluorescence collect and photoelectric converting device |
| CN209215224U (en) * | 2016-03-04 | 2019-08-06 | 国立大学法人九州大学 | Optical system tectosome and optical detecting device |
| CN210775219U (en) * | 2019-08-30 | 2020-06-16 | 广州蓝勃生物科技有限公司 | Installer and parallel fluorescence detection module with different wavelengths |
| CN212321442U (en) * | 2020-08-12 | 2021-01-08 | 济南国益生物科技有限公司 | Multi-fluorescence channel detection system for real-time fluorescence quantitative PCR |
-
2020
- 2020-08-12 CN CN202010806289.1A patent/CN111781185A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0943132A (en) * | 1995-07-27 | 1997-02-14 | Fujitsu Ltd | Particle measuring instrument |
| CN209215224U (en) * | 2016-03-04 | 2019-08-06 | 国立大学法人九州大学 | Optical system tectosome and optical detecting device |
| CN105675574A (en) * | 2016-03-17 | 2016-06-15 | 苏州天隆生物科技有限公司 | Multi-fluorescence channel detection system for real-time fluorescence quantitative PCR |
| CN108593554A (en) * | 2018-05-24 | 2018-09-28 | 大连民族大学 | It is a kind of can multidimensional adjust laser induced fluorescence collect and photoelectric converting device |
| CN210775219U (en) * | 2019-08-30 | 2020-06-16 | 广州蓝勃生物科技有限公司 | Installer and parallel fluorescence detection module with different wavelengths |
| CN212321442U (en) * | 2020-08-12 | 2021-01-08 | 济南国益生物科技有限公司 | Multi-fluorescence channel detection system for real-time fluorescence quantitative PCR |
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