CN111778351A - Specific molecular marker primer and kit for black fungus strain and application of specific molecular marker primer and kit - Google Patents
Specific molecular marker primer and kit for black fungus strain and application of specific molecular marker primer and kit Download PDFInfo
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- 241000233866 Fungi Species 0.000 title claims abstract description 75
- 239000003147 molecular marker Substances 0.000 title claims abstract description 20
- 238000011144 upstream manufacturing Methods 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims description 16
- 108020004414 DNA Proteins 0.000 claims description 13
- 238000012408 PCR amplification Methods 0.000 claims description 12
- 239000012634 fragment Substances 0.000 claims description 10
- 235000000023 Auricularia auricula Nutrition 0.000 claims description 8
- 241001149430 Auricularia auricula-judae Species 0.000 claims description 8
- 241000221377 Auricularia Species 0.000 claims description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 3
- 238000000137 annealing Methods 0.000 claims description 3
- 238000004925 denaturation Methods 0.000 claims description 3
- 230000036425 denaturation Effects 0.000 claims description 3
- 150000007523 nucleic acids Chemical group 0.000 claims description 3
- 238000012257 pre-denaturation Methods 0.000 claims description 3
- 230000001351 cycling effect Effects 0.000 claims 1
- 230000003321 amplification Effects 0.000 abstract description 6
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 2
- 238000004321 preservation Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000001502 gel electrophoresis Methods 0.000 description 3
- 239000012154 double-distilled water Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 241000269913 Pseudopleuronectes americanus Species 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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Abstract
The invention discloses a specific molecular marker primer and a kit of black fungus strains and application thereof, wherein the primer pair is named as 3-ty 026-3; the sequence of the upstream primer is 5'-CGAGAGGCAAGGTGAACTCC-3'; the sequence of the downstream primer is 5'-GCTGGCAGTCGATCAAGATC-3'. The molecular marker primer pair 3-ty026-3 of the invention has strong specificity, can perform specific amplification on the genomic DNA of the black fungus strain Guiyun No. 3, has no amplification effect on other black fungus strains, and can quickly and accurately identify the black fungus strain Guiyun No. 3 by utilizing the specific molecular marker primer pair 3-ty 026-3.
Description
Technical Field
The invention relates to the technical field of microorganisms, and particularly relates to a specific molecular marker primer and a kit for black fungus strains and application of the specific molecular marker primer and the kit.
Background
The black fungus strain Guiyun No. 3 is a new strain obtained by separating, domesticating and culturing Baicai wild black fungus. The strain has high fruiting body soaking rate (above 20.0), and is rich in ferrum (above 166 mg/Kg) and polysaccharide (4.15g/100 g); the strain sporocarp is in a single-sheet shape, has no vein or few vein, is soft and elastic in ear, smooth and smooth in mouth feel and is deeply favored by consumers. The fresh product is yellow brown, bright and transparent; the dried product has dark brown abdominal surface, gray black back surface, excellent appearance and wide market prospect, and the strain is patented (patent application number: 20181126406.2). At present, the identification of black fungus strains is generally carried out on the aspects of shape, properties and physiological characteristics, and the identification of the black fungus strains is not accurate due to the influence of planting environment and artificial subjective factors. Therefore, the research of the effective identification method of the black fungus strain Guiyun No. 3 has important significance for further genetic improvement research and popularization and cultivation in production of the strain.
The invention aims at designing specific primers on the basis of the original ISSR genetic difference analysis, establishes the Guiyun No. 3 SCAR molecular marker, and can simply, quickly and clearly identify the strain by utilizing the molecular marker.
Disclosure of Invention
The invention overcomes the technical problem that the identification of black fungus strains is inaccurate by using the form, the character and the physiological characteristics in the prior art, and provides a specific molecular marker primer and a kit for black fungus strains and application thereof.
In order to solve the problems, the invention adopts the following technical scheme:
a molecular marker primer pair, which is named 3-ty 026-3; the sequence of the upstream primer is 5'-CGAGAGGCAAGGTGAACTCC-3'; the sequence of the downstream primer is 5'-GCTGGCAGTCGATCAAGATC-3'.
The second purpose of the invention is also to protect a kit, and the kit comprises the primer pair.
The kit also included 2 × Es TaqMasterMix, ddH 2O.
The third purpose of the invention is to protect the application of the primer pair or the kit in identifying the black fungus strain Guiyun No. 3.
Wherein, the black fungus strain Guiyun No. 3 (Auricularia auricular-judae) of the invention has been preserved in China general microbiological culture Collection center, the address is: the No. 3 Xilu Beijing, Chaoyang, is No.1 Hospital, with the preservation number of CGMCC No.15367 and the preservation date of 3 months and 22 days in 2018.
The fourth purpose of the invention is to protect a method for identifying black fungus strain Guiyun No. 3 from a plurality of black fungus strains by using the primer pair 3-ty026-3, wherein the method comprises the following steps: extracting total DNA of the black fungus strain to be identified, carrying out PCR amplification on the 3-ty026-3 by using the primer pair, and judging the strain to be black fungus strain Guiyun No. 3 when a 445bp fragment is amplified.
In the method, the reaction procedure of the PCR amplification is as follows: pre-denaturation at 95 deg.C for 5min, denaturation at 95 deg.C for 30sec, annealing at 60 deg.C for 30sec, extension at 72 deg.C for 60sec, circulating for 30 times, extension at 72 deg.C for 7min, and storing at 4 deg.C for 30 min; the PCR reaction system adopted during PCR amplification is calculated by 25 mu L as follows: 2 XEs TaqMasterMix 11.5 mu L, ddH2O 10.5.5 mu L, 10 mu mol/L upstream primer 1 mu L, 10 mu mol/L downstream primer 1 mu L and black fungus strain genome DNA1 mu L to be detected.
The nucleic acid sequence of the specific fragment amplified by the primer pair 3-ty026-3 is shown as the sequence table SEQ ID NO. 2.
Compared with the prior art, the invention has the following beneficial effects: the primer pair 3-ty026-3 of the invention has strong specificity, can perform specific amplification on DNA extracted from black fungus strain Guiyun No. 3, can amplify a 445bp segment, and has no amplification effect on other black fungus strains. By utilizing the primer pair 3-ty026-3, the black fungus strain Guiyun No. 3 can be simply, conveniently, quickly and accurately screened and identified according to the molecular marker screening and identifying method, and the method has important significance for market popularization of the black fungus strain Guiyun No. 3.
Drawings
FIG. 1 is a gel electrophoresis diagram of PCR amplification of a 3-ty026-3 molecular marker primer of the invention;
FIG. 2 is a gel electrophoresis diagram of another PCR amplification of the 3-ty026-3 molecular marker primer of the invention;
FIG. 3 is a gel electrophoresis diagram of another PCR amplification of the 3-ty026-3 molecular marker primer of the invention;
Detailed Description
The present invention will be further described with reference to examples and tests.
Example 1
A molecular marker primer pair, which is named 3-ty 026-3; the sequence of the upstream primer is 5'-CGAGAGGCAAGGTGAACTCC-3'; the sequence of the downstream primer is 5'-GCTGGCAGTCGATCAAGATC-3'.
Example 2
The primer pair 3-ty026-3 is used in identifying Auricularia strain Guiyun No. 3. Wherein the preservation number of the black fungus strain Guiyun No. 3 is CGMCC No. 15367.
Example 3
A method for identifying black fungus strain Guiyun No. 3 from a plurality of black fungus strains by using the primer pair 3-ty026-3 comprises the following steps: extracting total DNA of the black fungus strain to be screened, carrying out PCR amplification on the 3-ty026-3 by using the primer pair, and judging the strain to be black fungus strain Guiyun No. 3 when a 445bp fragment is amplified.
In the method, the reaction procedure of the PCR amplification is as follows: pre-denaturation at 95 deg.C for 5min, denaturation at 95 deg.C for 30sec, annealing at 60 deg.C for 30sec, extension at 72 deg.C for 60sec, circulating for 30 times, extension at 72 deg.C for 7min, and storing at 4 deg.C for 30 min;
in the method, the PCR reaction system adopted during PCR amplification is calculated by 25 mu L as follows: 2 XEsTaqMasterMix 11.5 mu L, ddH2O 10.5.5 mu L, 10 mu mol/L upstream primer 1 mu L, 10 mu mol/L downstream primer 1 mu L, and 1 mu L of black fungus strain genome DNA to be detected.
Example 4
The skilled person can design a kit containing the primer pair for identifying the black fungus strain Guiyun No. 3 according to the primer pair of the invention, therefore, another object of the invention is to protect a kit comprising the primer pair 3-ty026-3, 2 XEs TaqMasterMix and ddH 2O.
The kit is used for detecting the black fungus strain Guiyun No. 3. Wherein the preservation number of the black fungus strain Guiyun No. 3 is CGMCC No. 15367.
Primer pair 3-ty026-3 specificity experiment:
the invention uses the primer pair 3-ty026-3, uses different black fungus variety genome DNA as template, verifies whether the primer can only carry out specific amplification to Guiyun No. 3 genome DNA, the amplification method adopts the method for identifying black fungus strain Guiyun No. 3, the result is shown in figure 1-3:
in FIGS. 1-3, all the markers used are DL2000, which is 2000bp, 1000bp, 750bp, 500bp, 250bp and 100bp from top to bottom.
The black fungus strains 1-4 in the figure 1 are respectively as follows: black fungus strain Guiyun No. 3, black fungus strain ty071, black fungus strain 916, and black fungus strain Au 8129;
the black fungus strains 1-8 in the figure 2 are respectively as follows: black fungus strain Guiyun No. 3, black fungus strain ty013, black fungus strain ty041, black fungus strain ty051, black fungus strain ty063, black fungus strain YE3, black fungus strain ty071 and black fungus strain YE 1;
the black fungus strains 1-12 in the figure 3 are respectively as follows: black fungus strain Guiyun No. 3, black fungus strain 916, black fungus strain New family No. 10, black fungus strain New family No. 4, black fungus strain Black three, black fungus strain Baiyun No. 6, black fungus strain Au8129, black fungus strain Au149, black fungus strain YE2, black fungus strain Black 29, black fungus strain Black fungus No. 2 and black fungus strain Tianlin black fungus 181.
As can be seen from FIGS. 1 to 3, except that a specific band appears at a position of 445bp in a lane 1 (i.e., a sample is the Auricularia auricula-judae strain Guiyun No. 3), no specific band appears in other lanes, so that the primer pair 3-ty026-3 of the application can have specific identification degree on the Auricularia auricula-judae strain Guiyun No. 3, and can distinguish the Auricularia auricula-judae strain Guiyun from other Auricularia auricula-judae strains.
Sequencing the DNA fragment obtained by amplifying the primer pair 3-ty026-3, and obtaining a fragment with the size of 445bp, wherein the fragment is contained in the DNA fragment recovered from the gel, and the nucleic acid sequence of the specific fragment is shown as a sequence table SEQ ID NO: 2.
In the above experiment, the sources of the black fungus strains used are shown in the following table 1:
TABLE 1
The above description is intended to describe in detail the preferred embodiments of the present invention, but the embodiments are not intended to limit the scope of the claims of the present invention, and all equivalent changes and modifications made within the technical spirit of the present invention should fall within the scope of the claims of the present invention.
Sequence listing
<110> institute of microbiology of Guangxi Zhuang autonomous region academy of agricultural sciences
<120> specific molecular marker primer and kit for black fungus strain and application thereof
<160>3
<170>SIPOSequenceListing 1.0
<210>1
<211>20
<212>DNA
<213> Black fungus (Auricularia auricula-judae)
<400>1
cgagaggcaa ggtgaactcc 20
<210>2
<211>445
<212>DNA
<213> Black fungus (Auricularia auricula-judae)
<400>2
cgagaggcaa ggtgaactcc cagcccgggg ggagaatata tacatcaaag tgccacaatg 60
taagcggaac aaaggaatac acatttggtg taacactaga cgcacatccc tatcttgaag 120
catcagtaca aggagaactg ccaatatggg gccattttgc acttgagaaa tcaccaagga 180
aatctgccag cactgcgtgc ttacttgata atcataagtg caaaacagtc tctgacgctt 240
gcgccctagc atctatcctc gactcaaatt ggcacaggaa caataagaac tgtatatgtc 300
gcaaatgcaa ccaaattagg cagagcacaa cttgtaataa tccttataga tgtgcgaaag 360
cagccaaaaa gattctggat agccttctac caaaatggaa tccccttaaa cgacctccac 420
actatgatct tgatcgactg ccagc 445
<210>3
<211>20
<212>DNA
<213> Black fungus (Auricularia auricula-judae)
<400>3
gctggcagtc gatcaagatc 20
Claims (7)
1. A molecular marker primer pair, which is named as 3-ty 026-3; the sequence of the upstream primer is 5'-CGAGAGGCAAGGTGAACTCC-3'; the sequence of the downstream primer is 5'-GCTGGCAGTCGATCAAGATC-3'.
2. A kit comprising the primer pair of claim 1.
3. The application of the primer pair described in claim 1 and/or the kit described in claim 2 in identifying Auricularia auricula-judae strain Guiyun No. 3.
4. The use of claim 3, wherein the Auricularia auricular strain Guiyun No. 3 has a deposit number of: CGMCC No. 15367.
5. A method for identifying black fungus strain Guiyun No. 3 from a plurality of black fungus strains by using the primer pair of claim 1 is characterized by comprising the following steps: extracting total DNA of the black fungus strain to be identified, carrying out PCR amplification on the 3-ty026-3 by using the primer pair, and judging the strain to be black fungus strain Guiyun No. 3 when a 445bp fragment is amplified.
6. The method of claim 5, wherein the PCR amplification reaction is performed by pre-denaturation at 95 ℃ for 5min, denaturation at 95 ℃ for 30sec, annealing at 60 ℃ for 30sec, extension at 72 ℃ for 60sec, cycling for 30 times, extension at 72 ℃ for 7min, and storage at 4 ℃ for 30min, and the PCR reaction system used in the PCR amplification reaction is 2 × Es TaqMasterMix 11.5. mu. L, ddH (measured in 25. mu.L)210.5 mul of O, 1 mul of 10 mul mol/L upstream primer, 1 mul of 10 mul mol/L downstream primer and 1 mul of genome DNA of the black fungus strain to be detected.
7. The method of claim 5, wherein the nucleic acid sequence of the specific fragment amplified by the primer pair 3-ty026-3 is shown as SEQ ID NO. 2 of the sequence Listing.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114540535A (en) * | 2022-03-24 | 2022-05-27 | 广西壮族自治区农业科学院 | Molecular marker for rapidly identifying auricularia polytricha and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101475991B (en) * | 2009-01-19 | 2011-08-24 | 黑龙江省科学院微生物研究所 | Method for identifying black fungus bacterial strain 185 and gene sequence for identifying black fungus bacterial strain 185 |
| KR101217713B1 (en) * | 2011-12-23 | 2012-12-31 | 홍운표 | Methods for fermentative production of fibrinolytic enzyme from Auricularia auricula－judae and uses thereof |
| CN105950777A (en) * | 2016-07-18 | 2016-09-21 | 黑龙江省科学院微生物研究所 | Method for rapidly and accurately authenticating and evaluating black fungus germplasm resources and black 29 variety specific molecular identity card |
| CN106755531A (en) * | 2017-02-27 | 2017-05-31 | 吉林农业大学 | A kind of optimizing reaction system of black fungus SSR PCR |
| CN107285877A (en) * | 2017-07-14 | 2017-10-24 | 广西科学院生物研究所 | Culture medium, cultural method and the bacterium rod grand style structure cultivated for open field cloud ear |
| CN109220530A (en) * | 2018-10-29 | 2019-01-18 | 广西壮族自治区农业科学院微生物研究所 | A kind of black fungus strain and its application |
-
2020
- 2020-07-31 CN CN202010755970.8A patent/CN111778351B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101475991B (en) * | 2009-01-19 | 2011-08-24 | 黑龙江省科学院微生物研究所 | Method for identifying black fungus bacterial strain 185 and gene sequence for identifying black fungus bacterial strain 185 |
| KR101217713B1 (en) * | 2011-12-23 | 2012-12-31 | 홍운표 | Methods for fermentative production of fibrinolytic enzyme from Auricularia auricula－judae and uses thereof |
| CN105950777A (en) * | 2016-07-18 | 2016-09-21 | 黑龙江省科学院微生物研究所 | Method for rapidly and accurately authenticating and evaluating black fungus germplasm resources and black 29 variety specific molecular identity card |
| CN106755531A (en) * | 2017-02-27 | 2017-05-31 | 吉林农业大学 | A kind of optimizing reaction system of black fungus SSR PCR |
| CN107285877A (en) * | 2017-07-14 | 2017-10-24 | 广西科学院生物研究所 | Culture medium, cultural method and the bacterium rod grand style structure cultivated for open field cloud ear |
| CN109220530A (en) * | 2018-10-29 | 2019-01-18 | 广西壮族自治区农业科学院微生物研究所 | A kind of black fungus strain and its application |
Non-Patent Citations (3)
| Title |
|---|
| FANG-JIE YAO等: "development of a molecular marker for fruiting body pattern in auricularia auricula-judae", 《MYCOBIOLOGY》 * |
| 唐利华等: "中国黑木耳主要栽培菌株ISSR指纹分析及SCAR标记", 《菌物学报》 * |
| 祁亮亮等: "广西云耳物种学名探究", 《资源调查》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114540535A (en) * | 2022-03-24 | 2022-05-27 | 广西壮族自治区农业科学院 | Molecular marker for rapidly identifying auricularia polytricha and application thereof |
| CN114540535B (en) * | 2022-03-24 | 2023-11-28 | 广西壮族自治区农业科学院 | Molecular marker for rapidly identifying auricularia polytricha and application thereof |
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