CN111778184A - Microbial agent suitable for industrial large-scale compost and application thereof - Google Patents
Microbial agent suitable for industrial large-scale compost and application thereof Download PDFInfo
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention belongs to the field of microorganisms and application thereof, and particularly discloses a microbial agent suitable for industrial large-scale compost and application thereof, wherein the compound microbial agent can be used for compost fermentation of livestock and poultry manure and comprises the following components: bacillus subtilis (BS 1), bacillus amyloliquefaciens (Ba), bacillus licheniformis (L3), and yeast (Y2). Wherein, the Ba strain is preserved in the China center for type culture Collection with the preservation number: CCTCC M2020098. The composite microbial inoculum can improve the composting temperature, accelerate the degradation of organic substances, promote the nitrogen conversion process, shorten the composting period, fix nitrogen and improve the fertilizer efficiency of organic fertilizers in composting fermentation.
Description
Technical Field
The invention belongs to the field of microorganisms and application thereof, and particularly relates to a complex microbial inoculum and application thereof; the complex microbial inoculum can be used for composting fermentation of livestock and poultry manure.
Background
With the continuous expansion of the total livestock and poultry breeding amount in China, the treatment of the livestock and poultry feces becomes a great problem. According to industry statistics, about 38 hundred million tons of livestock and poultry manure is generated every year in China, wherein about 18 million tons of manure directly excreted by livestock and poultry. The livestock and poultry manure is large in discharge quantity and high in treatment difficulty, becomes one of the sources of agricultural non-point source pollution, seriously influences the health and production performance of livestock and poultry, and causes huge pressure on surrounding water bodies and ecological environment, thereby becoming a bottleneck restricting the sustainable development of animal husbandry. Although the county propulsion scheme of recycling the livestock and poultry manure is implemented nationwide in recent years and measures such as producing organic fertilizers to replace chemical fertilizers are encouraged, the recycling utilization rate of the livestock and poultry wastes is still less than 60%, and the organic fertilizer products still have the problems of weak functions, poor effects, low use enthusiasm of farmers, difficult popularization and the like.
Aerobic composting is one of the most widely and effectively applied treatment modes for treating livestock and poultry manure at present, and still has the problems of slow temperature rise, long fermentation time, low composting quality, serious nitrogen loss, low humification degree, obvious emission of ammonia gas and greenhouse gas and the like. Especially, the odor pollution is serious, the nitrogen loss is serious, and the nitrogen loss in the composting process is as high as 33-60 percent, so that the fertilizer efficiency of the organic fertilizer product is low. How to optimize the composting process, strengthen the composting process, improve the production efficiency of organic fertilizer and the product quality, improve the use enthusiasm of farmers and accelerate the replacement of the organic fertilizer for chemical fertilizer is a problem which is urgently needed to be solved at present. Researchers have long conducted a great deal of research around these problems, such as optimization of composting parameters and processes, addition of bulking agents, initiators, conditioners, microbial inoculants, and the like to fix nitrogen and deodorize, and increase composting efficiency and quality. Among them, the reports of inoculated microorganisms are the most, but the application effect of inoculation is not uniform.
The essence of the composting is the metabolism of microorganisms under appropriate conditions, and the process of optimizing the composting process is actually the process of optimizing the metabolism of microorganisms in the composting process. The original purpose of adding exogenous microbial agents is as follows: improving microbial population at the initial stage of composting and enhancing the degradation activity of microbes; secondly, the time for reaching high temperature is shortened; inoculating bacterial strain with strong organic matter decomposing capacity. Research shows that the addition of exogenous microbial inoculum can increase the number of microorganisms in the compost at the initial stage, so as to accelerate the composting reaction process and quickly treat the livestock and poultry manure (Nakasaki et al, 2013). Particularly, the cellulose degradation flora with specific functions is inoculated in the composting process, so that the decomposition of cellulose organic matters in the compost can be accelerated, and the humification degree and the total microbial biomass of a composting finished product can be improved (Z eng et al, 2010; Huangdan lotus, 2011; Linmin, 2015; Wuqingshan, 2018). Mao et al (2018) composted in a laboratory scale glass flask reactor and found that the addition of complex bacterial powder improved nitrogen storage and microbial communities in the compost. The Xujiaqi (2019) research shows that the bacillus composite inoculant is added into laboratory compost with the period of 45 days (turning over every 10 days) made of cow dung and sugarcane leaves, so that the nitrogen loss in the composting process can be remarkably reduced, and the degradation of organic matters is promoted. Guo et al (2020) performed a 43-day pig manure composting test using a laboratory scale composting reactor (130L effective volume), and found that the addition of Bacillus megaterium can promote the ammonia oxidation process in the compost and reduce the emission of ammonia nitrogen. Li et al (2020) found that the addition of Lactobacillus plantarum mixed with strains P-8 and LP-10 improved the efficiency of the sheep manure composting and the quality of the final product, but the whole composting period still required 56 days.
Although researchers have conducted a great deal of research on composting microbial agents, these studies have been mostly performed on a laboratory scale, the composting period is long, there are great differences in composting raw materials and process control in different studies, and these microbial agents also only have a certain strengthening effect on certain individual composting indexes, and the commercial application effect is limited. Therefore, the development of microbial inoculum products with remarkable application effects is the key for improving the composting process, strengthening the composting process and promoting the popularization and application of the microbial inoculum products in the production of commercial organic fertilizers.
In the large-scale fertilizer production process of batch production, the relatively universal composting system adopted at present mainly has three kinds: a strip stack type composting system, a mechanical reinforced trough type composting system and a closed bin type composting system. Wherein, according to the regulation of national livestock manure composting technical regulation NY/T2018, the fermentation period of the strip-stack compost is about 30 days, the fermentation temperature is maintained at 55 ℃ for more than 15 days, the water content of the finished product is less than 45 percent, the temperature is less than 35 ℃, no odor exists, and the germination index is more than 70 percent. However, in the production of organic fertilizers, due to the reasons that the proportion of compost raw materials and auxiliary materials is improper, the water content is too high, the rake-turning depth and times of the rake-turning machine are not enough or are influenced by external low temperature and the like, the conditions that the compost temperature is slow, the compost period is too long, the fertilizer efficiency of the organic fertilizers is low and the like often exist.
The applicant aims at the industrial demand, aims at solving the core problem behind the technical bottlenecks of production efficiency and quality of organic fertilizers, develops the compound microbial inoculum on the basis of years of research, verifies the application effect through laboratory small-sized composting and pilot-scale tests in organic fertilizer plants, and performs comparison tests with microbial inoculum sold in the market.
Disclosure of Invention
Aiming at the existing technologies, the invention aims to provide a microbial agent suitable for industrial large-scale compost, and the microbial agent comprises bacillus subtilis BS1(CCTCC NO: M2019185), bacillus amyloliquefaciens Ba, bacillus licheniformis L3 and saccharomyces cerevisiae Y2, wherein the preservation number of the bacillus amyloliquefaciens Ba is CCTCC N O: m2020098.
The invention also aims to provide the application of the microbial inoculum suitable for industrial large-scale compost, and the microbial inoculum can be used for livestock manure compost, can shorten the composting period, improve the composting efficiency, protect nitrogen and fix nitrogen, and improve the fertilizer efficiency of organic fertilizers.
In order to achieve the purpose, the invention adopts the following technical measures:
the applicant screens functional strains from existing strains separated from different habitats such as livestock and poultry manure, compost, soil and the like through strain enzyme production activity analysis, antibiotic sensitivity test, in-vitro bacteriostasis test, analysis on utilization capacity of different nitrogen sources and the like. Finally, the bacillus amyloliquefaciens Ba is screened out, and the strain is delivered to the China center for type culture Collection in 29 months at 2020, and is classified and named as follows: bacillus amyloliquefaciens Ba with a preservation number of: CCTCC NO: m2020098, address: wuhan university in Wuhan, China.
The microbial agent comprises bacillus subtilis BS1(CCTC C NO: M2019185), bacillus amyloliquefaciens Ba, bacillus licheniformis L3 and saccharomyces cerevisiae Y2, wherein the preservation number of the bacillus amyloliquefaciens Ba is CCTCC NO: m2020098.
The active ingredients of the complex microbial inoculum are mainly characterized as follows:
the composite microbial inoculum is prepared by mixing bacillus subtilis BS1(CCTCC NO: M2019185), bacillus amyloliquefaciens Ba, bacillus licheniformis L3 and saccharomyces cerevisiae Y2 according to the ratio of 3L:1-2L:3-4L: 4-5L.
Before the composite microbial inoculum is mixed, the effective bacteria concentration of the bacillus subtilis is (6.1-8.2) x108CFU/mL, effective bacteria concentration of bacillus amyloliquefaciens (2.6-3.07) x108CFU/mL, the effective bacteria concentration of the bacillus licheniformis is (1.17-1.7) x108CFU/mL, yeast effective concentration (4.8-6.2) x107CFU/mL；
The application of the microbial agent suitable for industrial large-scale compost comprises the application of the composite microbial agent in livestock and poultry manure compost, and is particularly suitable for the compost with the compost volume of more than or equal to 12 tons.
Compared with the prior art, the invention has the following advantages and effects:
(1) the composting period is obviously shortened, and the composting efficiency is improved;
(2) nitrogen is preserved and fixed, and fertilizer efficiency is improved.
(3) Promoting the fermentation and temperature rise of the compost.
(4) The complex microbial inoculum is particularly suitable for industrial large-scale fermentation.
Drawings
FIG. 1 is a graph showing the analysis of the ability of each strain of the present invention to utilize different nitrogen sources.
FIG. 2 is a diagram showing the results of in vitro bacteriostatic tests of various strains in the complex microbial inoculum.
FIG. 3 is a graph showing the effect of complex microbial inoculum on the temperature of a small compost pile in a laboratory.
FIG. 4 is a diagram of the change of the composite microbial inoculum in the nitrate nitrogen, ammonium nitrogen and total nitrogen content of the small-sized compost heap in the laboratory.
FIG. 5 is a graph showing the effect of the complex microbial inoculum on the nitrogenase gene in the composting process.
FIG. 6 is a graph showing the effect of microbial inoculum on composting temperature in the application of organic fertilizer plants.
FIG. 7 is a graph showing the effect of microbial inoculum on total nitrogen, total carbon and C/N content of compost in the application of organic fertilizer plants.
Detailed Description
The following examples facilitate a better understanding of the invention, but are not intended to limit the invention. The experimental procedures in the following examples are conventional unless otherwise specified. The reagents or materials, if not specifically mentioned, are commercially available.
Example 1:
screening a functional strain of bacillus amyloliquefaciens Ba:
the applicant screens functional strains from existing strains separated from different habitats such as feces, compost, soil and the like through enzyme production activity analysis of the strains, antibiotic sensitivity tests, in vitro bacteriostasis tests, utilization capacity analysis of different nitrogen sources and the like.
(1) Analysis of enzyme production Activity of Strain
Respectively inoculating the strains to be detected on a casein culture medium, a solid starch culture medium and a congo red cellulose culture medium flat plate, performing primary evaluation by comparing the ratio of the diameter of a transparent ring on the culture medium flat plate to the diameter of a bacterial colony, wherein the larger the ratio is, the higher the amylase activity is, and then comparing the amylase activity by combining the enzyme activities of bacterial liquids of the strains. The finally measured results of the strain Ba are shown in tables 1, 2 and 3, and the preservation number of the BS1 is CCTCC NO: m2019185.
TABLE 1 protease Activity of the strains
TABLE 2 Amylase Activity of the strains
TABLE 3 cellulase Activity of the strains
(2) Antibiotic susceptibility testing
Selecting antibacterial drug sensitive tablets such as tetracycline, chloramphenicol, erythromycin, norfloxacin, kanamycin, compound sulfamethoxazole and the like, and carrying out drug sensitive test by a paper diffusion method (K-B method). In an antibiotic susceptibility test, the diameter of the inhibition zone is more than 15mm, which indicates that the strain is highly sensitive to the antibiotic, the diameter of the inhibition zone is 10-15 mm, which indicates that the strain is moderately sensitive to the antibiotic, the inhibition zone is less than 10mm, which indicates that the strain is low sensitive to the antibiotic, and the absence of the inhibition zone indicates that the strain is a drug-resistant strain. As shown in Table 4 (the number represents the diameter of a zone of inhibition, unit cm), except BL1 which is low sensitive to erythromycin, BS5-21 which is medium sensitive to tetracycline and BL1 which is medium sensitive to erythromycin, the other strains are highly sensitive to common antibiotics, which indicates that the strains do not carry resistance genes (ARGs) and have good safety in the aspect of drug resistance.
TABLE 4 antibiotic susceptibility test of strains
(3) Analysis of utilization capacity of strains on different nitrogen sources
SC vs NH as shown in FIG. 13·H2O、KNO3、NaNO2The utilization of (A) is relatively high, which indicates that the SC has deodorization capacity; ba has strong utilization capacity to all nitrogen sources, wherein NH is utilized4The most prominent nitration of Cl is carried out; BL1 mainly uses KNO3Is used as a nitrogen source; BM pair (NH)4)2SO4、NH3·H2O is most preferably used.
(4) In vitro bacterial inhibition test
Respectively coating escherichia coli, staphylococcus aureus and salmonella on an LB solid culture medium, drawing a cross line at the bottom of a flat plate, punching the flat plate by using a puncher, sealing holes, adding bacterial liquid with the same volume in the holes, culturing at the constant temperature of 37 ℃ for 16-24 hours, observing an inhibition zone, and exploring in-vitro inhibition tests (1, CK; 2, BS 2; 3, Ba; 4, BL 1; 5, SC) of each strain in the compound bacterial agent by using a punching method, wherein Ba has obvious inhibition effects on escherichia coli (12.4 mm), staphylococcus aureus (20.0mm) and salmonella (14.3mm) as shown in figure 2.
Through the functional screening of the strains, the bacillus amyloliquefaciens Ba is finally selected. The strain is delivered to China center for type culture Collection in 29 months at 4 of 2020, and is classified and named as follows: bacillus amyloliquefaciens Bacillusamyloliquefac ies Ba with the preservation number: CCTCC NO: m2020098, address: wuhan university in Wuhan, China.
Example 2:
the preparation of the microbial compound inoculant applicable to industrial large-scale compost comprises the following steps:
using Bacillus subtilisBS1(CCTCC NO: M2019185), Bacillus amyloliquefaciens Ba, Bacillus licheniformis L3 (Zhao Guo Yuan, 2009) and Saccharomyces cerevisiae Y2 (C3: (C3))Jialiyan2019) in a volume ratio of 3:1:3: 4.
Before mixing, the effective bacteria concentration of the bacillus subtilis is (6.1-8.2) x108CFU/mL, effective bacteria concentration of bacillus amyloliquefaciens (2.6-3.07) x108CFU/mL, the effective bacteria concentration of the bacillus licheniformis is (1.17-1.7) x108CFU/mL, effective concentration of saccharomyces cerevisiae (4.8-6.2) x107CFU/mL。
The viable count of the mixed composite microbial inoculum is 7.60 × 108CFU/mL, used in the following examples.
Example 3:
the application of the compound microbial inoculum in the small compost of livestock and poultry manure in a farm comprises the following application processes:
the composting method used in the embodiment of the invention comprises the following steps:
in order to keep the temperature of the compost, the composting device is an EPS foam box with the height of 280mm, the length of 540mm, the width of 400mm, the volume of 60L and the thickness of 3 mm. The bottom of the foam box is fully paved with sawdust of about 3cm to serve as a buffer layer, a small hole is formed in the side face of the foam box so that the temperature can be measured conveniently, and the ventilation device conducts ventilation regularly to guarantee uniform ventilation.
The total volume of the pile is 50L, samples are collected according to the temperature of the pile, and the temperature, the C/N content, the nitrate nitrogen, the ammonium nitrogen and the change condition of the azotase gene of the pile are detected.
The experimental groups were as follows:
chicken manure control group (CM + CK): the chicken manure and the saw powder (the mass ratio of the chicken manure to the saw powder is 5:1, the wet weight is the same as that of the chicken manure, 500mL of sterile water is added, and the wet weight of the chicken manure is 17kg before the experiment.
Chicken manure test group (CM + AB): chicken manure, sawdust and composite microbial inoculum (the mass ratio of the chicken manure to the sawdust is 5:1, the addition amount of the composite microbial inoculum is 500mL, and the composite microbial inoculum is inoculated and added according to 1 percent of the total volume ratio of a pile body)
Pig manure control group (PM + CK): pig manure and saw dust (the mass ratio of the pig manure to the saw dust is 7:1, 500mL of sterile water is added, and the wet weight of the pig manure is 17kg
Pig manure test group (PM + AB): pig manure, sawdust and composite microbial inoculum (the mass ratio of the pig manure to the sawdust is 7:1, the adding amount of the composite microbial inoculum is 500mL, and the composite microbial inoculum is inoculated and added according to 1 percent of the total volume ratio of the pile body)
The composting test apparatus used in this study belongs to the reactor composting mode. According to the reactor composting process parameters specified in national livestock manure composting technical regulation NY/T2018, the reactor composting fermentation period is 5-10 days, the water content of the finished product is less than 45%, the temperature is less than 35 ℃, and no odor exists. The temperature change result of the pile is shown in FIG. 3, the temperature rise speed of the AB group added with the composite microbial inoculum is higher than that of the CK group, and the high temperature period is reached preferentially. The highest temperature of the pig manure compost AB group can reach 61.9, and the highest temperature of the CK group can reach 60.4. The chicken manure compost decreased to 35 ℃ on day 6, and the pig manure group decreased to 35 ℃ on day 10. The chicken manure group has short duration of high-temperature period and high cooling speed, and the composting period is obviously shorter than that of the pig manure group.
The total nitrogen, nitrate nitrogen and ammonium nitrogen contents of the compost are shown in figure 4, and NH is generated in the 2 nd high-temperature period of the compost4 +Under the condition that the utilization rates of the compound bacteria are similar, the ammonium nitrogen and the nitrate nitrogen in the AB group are higher than those in the CK group, which shows that the inoculated compound bacteria can accelerate the degradation of organic substances and promote the nitrogen conversion process. Cooling period NH4 +The nitrogen source utilized or converted by the microorganism to other forms rapidly decreases, and the decrease rate of the AB group is higher than that of the CK group. When the composting is finished, the ammonium nitrogen, nitrate nitrogen and total nitrogen in the AB group are all higher than those in the CK group, and the nitrogen source exists in the compost body in a relatively stable form, which indicates that the added compound microbial inoculum has the nitrogen fixation effect. The ammonium nitrogen and nitrate nitrogen of the chicken manure group added with the compound microbial inoculum are obviously higher than those of the pig manure group.
Nitrate nitrogen change (g/kg)
Heap ammonium nitrogen change (g/kg)
Heap total N variation (g/kg)
The cyclic conversion of nitrogen is an important component for analyzing nitrogen retention and fixation of the compost, and mainly comprises ammoxidation, nitrification, denitrification and nitrogen fixation. The nifH gene can fix nitrogen to form organic nitrogen. The expression level of nifH gene is detected by absolute fluorescent quantitative PCR, and the primers are as follows: nifH: f: 5 '-TGCGAYCSAARGCGCACTC-3', R: 5 '-ATSGCCATCATYTCRCCGGA-3'. The expression change of nifH genes is shown in figure 5, the change of the azotase genes in the composting process shows a trend of rising-falling, and the nifH abundance is obviously reduced in the cooling period. The nifH gene of the AB group azotase is higher than that of the CK group, and the azotobacter effect on chicken manure is more obvious than that of pig manure.
Example 4:
the pilot-scale application effect research of the compound microbial inoculum in an organic fertilizer plant comprises the following steps:
the compost fermentation material ratio and grouping:
mixing chicken manure and auxiliary materials according to the weight percentage of the chicken manure: shell and bran: after mushroom residues are fully and uniformly mixed by a stirrer according to the mass ratio of 7:2:1 (wet weight), 5 groups are arranged, wherein the groups are as follows:
blank group CK group (12 ton mixed material +5 liter water)
Compound bacteria preparation T1 group (12 ton mixed material +5L concentrated compound bacteria liquid (effective bacteria concentration of concentrated bacteria liquid is 1.52 × 10)9cfu/mL))，
Market liquid bacterial agent T2 group (12 ton material +5L liquid bacterial agent)
Market bacteria powder T3 group (12 ton material +13 jin bacteria powder +5L water)
Market bacteria powder T4 group (12 ton material +24 jin bacteria powder +5L water)
The addition amount of the market microbial inoculum is determined according to the instruction of the instruction. The product specification of the market microbial inoculum T2 shows that the microbial inoculum can deodorize and promote compost maturity. The main component of the microbial inoculum T3 product is bacillus licheniformis, the number of bacteria is 100 hundred million/g, and the microbial inoculum T3 product can be heated and can promote compost maturity. The product name of the microbial inoculum T4 is an organic material decomposing agent, the number of bacteria is 40 hundred million/g, the formula contains composite bacteria such as bacillus subtilis, saccharomycetes and the like, and the temperature rise of compost can be promoted, and the material is decomposed.
And (3) performing strip-stack composting on each group, wherein the height of a compost body is about 1.2 meters, the width is about 3 meters, and the length is about 8 meters, and turning and harrowing are performed for 1-2 times per day by using a harrowing machine until the chicken manure compost is thoroughly decomposed.
The temperature change of the compost is shown in fig. 6, and by monitoring the temperature, on the 1 st day of composting, the temperature of a T1 microbial inoculum addition group can reach more than 50 ℃, the temperature is higher, and the temperature is obviously higher than that of a control group; the third day can reach the national specified high temperature period of 55 ℃; the T1 group began to cool on day 19, and had reached the maturity temperature on day 27, while the other groups began to cool on day 38 and were nearly mature on day 41. Compared with a control group, the T1 group added with the compound microbial inoculum can obviously shorten the composting period.
The change of the water content of the compost in Table 5 was adjusted according to the water content and carbon-nitrogen ratio of each auxiliary material. The water content of the T1 group is stabilized at 33.27 percent when the compost is used for 27 days, which is obviously lower than that of other groups, and simultaneously the requirement of the water content of the compost decomposed sample is also met. And CK group did not drop to 29.95% until 41 days.
TABLE 5 stack moisture Change
The germination rate of the seeds is an index for evaluating the compost maturity. The germination rates of the seeds of samples at the composting time of 27 days are shown in the following table 6, the germination rates of the seeds of the T1 group are obviously higher than those of a blank control group and other market microbial inoculum groups, and the seeds reach the rotten and harmless levels specified by the state. When the compost is carried out for 41 days, the CK group is decomposed, the germination rate of compost sample seeds reaches 76.22%, and other market microbial inoculum groups reach the decomposition standard.
TABLE 6 Germination index of Brassica campestris seeds of compost sample on day 27
Note: CK, a control group, T1, a complex microbial inoculum adding group, and T2, T3 and T4 are market selling microbial inoculum adding groups with different sources respectively.
TABLE 7 Germination index of Brassica campestris seeds for compost sample on day 41
The change of the heap C/N, TN is shown in FIG. 7, when the samples of the heaps 0d, 2d, 27d and 41d are detected, the total nitrogen content is firstly reduced and then increased, mainly because nitrogen is lost in the form of ammonia gas during the temperature rise and high temperature period, the relative content of nitrogen is increased after composting is finished, and the content of the T1 nitrogen is obviously higher than that of CK group, which indicates that the composite microbial inoculum plays a role in nitrogen fixation. The change of the carbon-nitrogen ratio in the composting process shows a trend of increasing firstly and then decreasing secondly, when the compost is carried out for 27 days, the carbon-nitrogen ratio of the T1 group is 12.53, the carbon-nitrogen ratio of the CK group is 16.21, the T1 is thoroughly decomposed firstly, and the C-nitrogen ratio of the CK group and other market bactericides are decreased to the range of the rotten value after being added into the group for 41 days, which shows that the inoculated composite bactericides can promote the degradation of organic substances, shorten the composting period and achieve the effect of fixing nitrogen.
Stack C/N variation
Variation of stack N content
Claims (4)
1. The microbial agent comprises bacillus subtilis BS1, bacillus amyloliquefaciens Ba, bacillus licheniformis L3 and saccharomyces cerevisiae Y2, wherein the preservation number of the bacillus amyloliquefaciens Ba is CCTCC NO: m2020098, the preservation number of the bacillus subtilis BS1 is CCTCC NO: m2019185.
2. The microbial inoculant according to claim 1, wherein: mixing the bacillus subtilis BS1, the bacillus amyloliquefaciens Ba, the bacillus licheniformis L3 and the saccharomyces cerevisiae Y2 bacterial liquid according to the proportion of 3L to 1-2L to 3-4L to 4-5L; before mixing, the effective bacteria concentration of the bacillus subtilis is (6.1-8.2) x108CFU/mL, effective bacteria concentration of bacillus amyloliquefaciens (2.6-3.07) x108CFU/mL, the effective bacteria concentration of the bacillus licheniformis is (1.17-1.7) x108CFU/mL, yeast effective concentration (4.8-6.2) x107CFU/mL。
3. Use of the microbial inoculant of claim 1 in industrial mass composting.
4. Use according to claim 3, the compost being on a scale equal to or greater than 12 tons.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112195138A (en) * | 2020-11-06 | 2021-01-08 | 河南省农业科学院植物营养与资源环境研究所 | Biological agent for livestock and poultry manure composting and preparation method thereof |
| CN115215690A (en) * | 2021-04-16 | 2022-10-21 | 中国农业大学 | Livestock manure treatment method, microbial inoculum and application |
| CN115216415A (en) * | 2021-04-16 | 2022-10-21 | 中国农业大学 | Microbial inoculum and application thereof in preparation of fertilizer |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103497915A (en) * | 2013-09-22 | 2014-01-08 | 东北农业大学 | Complex microbial agent for composting fermentation and application thereof |
| CN103980047A (en) * | 2014-05-19 | 2014-08-13 | 中国水稻研究所 | Microbial fertilizer for resisting damping off of rice seedlings and preparation method thereof |
| US20150258150A1 (en) * | 2014-03-14 | 2015-09-17 | Osprey Biotechnics, Inc. | Bacillus amyloliquefaciens strain |
| CN105219669A (en) * | 2015-11-20 | 2016-01-06 | 华中农业大学 | There is resistance of oxidation, promote the bacillus amyloliquefaciens TL that growth of meat chicken is grown |
| CN105255761A (en) * | 2015-10-23 | 2016-01-20 | 武汉骏安生物科技有限公司 | Deodorization fungicide for treating organic waste materials and application method thereof |
| CN106811435A (en) * | 2017-03-31 | 2017-06-09 | 甘肃农业大学 | A kind of Alpine Grasslands herbage endogenetic bacteria Bacillus amyloliquefaciens strain, microbial bacterial agent and its preparation method and application |
| CN107653200A (en) * | 2017-08-14 | 2018-02-02 | 华中农业大学 | A kind of microbial bacterial agent for promoting dead pig corpse aerobic compost and application |
| CN107988095A (en) * | 2017-11-27 | 2018-05-04 | 华中农业大学 | A kind of microbial bacterial agent of efficient degradation tetracycline antibiotics and application |
| WO2018152369A1 (en) * | 2017-02-15 | 2018-08-23 | Mcknight Gary David | Compositions and methods for coating of nitrification inhibitors with a solution containing a high concentration of urease inhibitors |
| CN110184218A (en) * | 2019-05-29 | 2019-08-30 | 华中农业大学 | A kind of composite bacteria agent and application based on bacillus subtilis BS1 |
| US20190343928A1 (en) * | 2017-03-09 | 2019-11-14 | Genofocus, Inc. | Bacillus amyloliquefaciens gf423 strain, and composition for providing antioxidant and anti-inflammatory activities or preventing or treating hyperlipidemia, including polypeptide produced by the same |
| CN110982760A (en) * | 2019-12-31 | 2020-04-10 | 云南省畜牧兽医科学院 | Bacillus belgii and application thereof in preventing and treating porcine diarrhea |
-
2020
- 2020-06-30 CN CN202010616986.0A patent/CN111778184B/en active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103497915A (en) * | 2013-09-22 | 2014-01-08 | 东北农业大学 | Complex microbial agent for composting fermentation and application thereof |
| US20150258150A1 (en) * | 2014-03-14 | 2015-09-17 | Osprey Biotechnics, Inc. | Bacillus amyloliquefaciens strain |
| CN103980047A (en) * | 2014-05-19 | 2014-08-13 | 中国水稻研究所 | Microbial fertilizer for resisting damping off of rice seedlings and preparation method thereof |
| CN105255761A (en) * | 2015-10-23 | 2016-01-20 | 武汉骏安生物科技有限公司 | Deodorization fungicide for treating organic waste materials and application method thereof |
| CN105219669A (en) * | 2015-11-20 | 2016-01-06 | 华中农业大学 | There is resistance of oxidation, promote the bacillus amyloliquefaciens TL that growth of meat chicken is grown |
| WO2018152369A1 (en) * | 2017-02-15 | 2018-08-23 | Mcknight Gary David | Compositions and methods for coating of nitrification inhibitors with a solution containing a high concentration of urease inhibitors |
| US20190343928A1 (en) * | 2017-03-09 | 2019-11-14 | Genofocus, Inc. | Bacillus amyloliquefaciens gf423 strain, and composition for providing antioxidant and anti-inflammatory activities or preventing or treating hyperlipidemia, including polypeptide produced by the same |
| CN106811435A (en) * | 2017-03-31 | 2017-06-09 | 甘肃农业大学 | A kind of Alpine Grasslands herbage endogenetic bacteria Bacillus amyloliquefaciens strain, microbial bacterial agent and its preparation method and application |
| CN107653200A (en) * | 2017-08-14 | 2018-02-02 | 华中农业大学 | A kind of microbial bacterial agent for promoting dead pig corpse aerobic compost and application |
| CN107988095A (en) * | 2017-11-27 | 2018-05-04 | 华中农业大学 | A kind of microbial bacterial agent of efficient degradation tetracycline antibiotics and application |
| CN110184218A (en) * | 2019-05-29 | 2019-08-30 | 华中农业大学 | A kind of composite bacteria agent and application based on bacillus subtilis BS1 |
| CN110982760A (en) * | 2019-12-31 | 2020-04-10 | 云南省畜牧兽医科学院 | Bacillus belgii and application thereof in preventing and treating porcine diarrhea |
Non-Patent Citations (2)
| Title |
|---|
| 周可等: "不同微生物菌剂处理对鸡粪堆肥发酵的影响", 《天津农业科学》 * |
| 易旻 等: "一株耐高温纤维素降解菌的分离筛选及鉴定", 《吉首大学学报（自然科学版）》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112195138A (en) * | 2020-11-06 | 2021-01-08 | 河南省农业科学院植物营养与资源环境研究所 | Biological agent for livestock and poultry manure composting and preparation method thereof |
| CN115215690A (en) * | 2021-04-16 | 2022-10-21 | 中国农业大学 | Livestock manure treatment method, microbial inoculum and application |
| CN115216415A (en) * | 2021-04-16 | 2022-10-21 | 中国农业大学 | Microbial inoculum and application thereof in preparation of fertilizer |
| CN115216415B (en) * | 2021-04-16 | 2023-12-26 | 中国农业大学 | Microbial inoculum and application thereof in preparation of fertilizer |
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