CN111742048A - 用于对单链核酸分子进行环化处理的夹板核酸分子及其应用 - Google Patents
用于对单链核酸分子进行环化处理的夹板核酸分子及其应用 Download PDFInfo
- Publication number
- CN111742048A CN111742048A CN201880088455.XA CN201880088455A CN111742048A CN 111742048 A CN111742048 A CN 111742048A CN 201880088455 A CN201880088455 A CN 201880088455A CN 111742048 A CN111742048 A CN 111742048A
- Authority
- CN
- China
- Prior art keywords
- nucleic acid
- acid molecule
- temperature
- stranded nucleic
- splint
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 266
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 266
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 266
- 230000000295 complement effect Effects 0.000 claims abstract description 112
- 239000012634 fragment Substances 0.000 claims abstract description 58
- 238000007363 ring formation reaction Methods 0.000 claims abstract description 49
- 238000012163 sequencing technique Methods 0.000 claims description 121
- 238000000034 method Methods 0.000 claims description 92
- 108020004414 DNA Proteins 0.000 claims description 68
- 102000003960 Ligases Human genes 0.000 claims description 50
- 108090000364 Ligases Proteins 0.000 claims description 50
- 238000006243 chemical reaction Methods 0.000 claims description 32
- 238000000746 purification Methods 0.000 claims description 32
- 230000029087 digestion Effects 0.000 claims description 31
- 238000000926 separation method Methods 0.000 claims description 26
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 23
- 238000004925 denaturation Methods 0.000 claims description 22
- 230000036425 denaturation Effects 0.000 claims description 22
- 239000011541 reaction mixture Substances 0.000 claims description 18
- 102000012410 DNA Ligases Human genes 0.000 claims description 15
- 108010061982 DNA Ligases Proteins 0.000 claims description 15
- 230000000694 effects Effects 0.000 claims description 15
- 230000008569 process Effects 0.000 claims description 15
- 238000012300 Sequence Analysis Methods 0.000 claims description 14
- 102000038379 digestive enzymes Human genes 0.000 claims description 13
- 108091007734 digestive enzymes Proteins 0.000 claims description 13
- 230000003321 amplification Effects 0.000 claims description 8
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 8
- 238000012408 PCR amplification Methods 0.000 claims description 7
- 230000008439 repair process Effects 0.000 claims description 7
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 6
- 108060002716 Exonuclease Proteins 0.000 claims description 4
- 102000013165 exonuclease Human genes 0.000 claims description 4
- 238000012545 processing Methods 0.000 claims description 4
- 238000002844 melting Methods 0.000 claims description 3
- 230000008018 melting Effects 0.000 claims description 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 2
- 238000003780 insertion Methods 0.000 claims 1
- 230000037431 insertion Effects 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 239000000047 product Substances 0.000 description 56
- 102000053602 DNA Human genes 0.000 description 26
- 239000011324 bead Substances 0.000 description 23
- 238000002156 mixing Methods 0.000 description 14
- 239000000203 mixture Substances 0.000 description 12
- 239000000243 solution Substances 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- 238000007664 blowing Methods 0.000 description 10
- 238000010276 construction Methods 0.000 description 10
- 239000000523 sample Substances 0.000 description 10
- 238000000137 annealing Methods 0.000 description 9
- 238000004153 renaturation Methods 0.000 description 9
- 108020004682 Single-Stranded DNA Proteins 0.000 description 8
- 239000007788 liquid Substances 0.000 description 8
- 239000011259 mixed solution Substances 0.000 description 8
- 239000006228 supernatant Substances 0.000 description 8
- 238000010586 diagram Methods 0.000 description 7
- 238000010009 beating Methods 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 239000007853 buffer solution Substances 0.000 description 6
- 239000002077 nanosphere Substances 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 239000002096 quantum dot Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000013382 DNA quantification Methods 0.000 description 3
- 108091034117 Oligonucleotide Proteins 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000005711 Benzoic acid Substances 0.000 description 2
- 238000001712 DNA sequencing Methods 0.000 description 2
- 101710163270 Nuclease Proteins 0.000 description 2
- 239000007984 Tris EDTA buffer Substances 0.000 description 2
- 239000012295 chemical reaction liquid Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 238000012165 high-throughput sequencing Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000007169 ligase reaction Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 108020004638 Circular DNA Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000004873 anchoring Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000012268 genome sequencing Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/501—Ligase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/204—Modifications characterised by specific length of the oligonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/107—Temperature of melting, i.e. Tm
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/113—Time
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
- C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
- C12Q2533/107—Probe or oligonucleotide ligation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
本发明提出了用于对单链核酸分子进行环化处理的夹板核酸分子及其应用。该夹板核酸分子由5’端片段和3’端片段组成，其中，所述5’端片段适于与所述单链核酸分子的5’末端形成第一互补区，所述3’端片段适于与所述单链核酸分子的3’末端形成第二互补区，以及所述第一互补区与所述第二互补区的长度不同。
Description
PCT国内申请，说明书已公开。
Claims (52)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2018/085086 WO2019205135A1 (zh) | 2018-04-28 | 2018-04-28 | 用于对单链核酸分子进行环化处理的夹板核酸分子及其应用 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111742048A true CN111742048A (zh) | 2020-10-02 |
| CN111742048B CN111742048B (zh) | 2024-02-20 |
Family
ID=68294711
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201880088455.XA Active CN111742048B (zh) | 2018-04-28 | 2018-04-28 | 用于对单链核酸分子进行环化处理的夹板核酸分子及其应用 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20210024933A1 (zh) |
| EP (1) | EP3789490A1 (zh) |
| CN (1) | CN111742048B (zh) |
| WO (1) | WO2019205135A1 (zh) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116287124A (zh) * | 2023-05-24 | 2023-06-23 | 中国农业科学院农业基因组研究所 | 单链接头预连接方法、高通量测序文库的建库方法及试剂盒 |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111455469B (zh) * | 2020-04-07 | 2023-08-18 | 深圳易倍科华生物科技有限公司 | 一种单链快速建库方法及建库仪器 |
| CN112111544B (zh) * | 2020-09-23 | 2022-04-01 | 复旦大学附属肿瘤医院 | 提高单链dna连接效率的方法 |
| WO2024036445A1 (zh) * | 2022-08-15 | 2024-02-22 | 深圳华大智造科技股份有限公司 | 一种测序文库的制备方法及用于制备测序文库的试剂盒 |
| CN116497088A (zh) * | 2023-04-26 | 2023-07-28 | 苏州吉因加生物医学工程有限公司 | 一种夹板核酸分子及其试剂盒 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000063437A2 (en) * | 1999-04-20 | 2000-10-26 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
| CN105986324A (zh) * | 2015-02-11 | 2016-10-05 | 深圳华大基因研究院 | 环状小rna文库构建方法及其应用 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4287652B2 (ja) * | 2000-10-24 | 2009-07-01 | ザ・ボード・オブ・トラスティーズ・オブ・ザ・レランド・スタンフォード・ジュニア・ユニバーシティ | ゲノムｄｎａの直接多重処理による性状分析 |
| US7862999B2 (en) * | 2007-01-17 | 2011-01-04 | Affymetrix, Inc. | Multiplex targeted amplification using flap nuclease |
-
2018
- 2018-04-28 CN CN201880088455.XA patent/CN111742048B/zh active Active
- 2018-04-28 US US17/043,447 patent/US20210024933A1/en active Pending
- 2018-04-28 EP EP18916138.3A patent/EP3789490A1/en not_active Withdrawn
- 2018-04-28 WO PCT/CN2018/085086 patent/WO2019205135A1/zh active Search and Examination
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000063437A2 (en) * | 1999-04-20 | 2000-10-26 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
| CN105986324A (zh) * | 2015-02-11 | 2016-10-05 | 深圳华大基因研究院 | 环状小rna文库构建方法及其应用 |
Non-Patent Citations (2)
| Title |
|---|
| GIUSEPPE VILLANI等: "Gap-Directed Translesion DNA Synthesis of an Abasic Site on Circular DNA Templates by a Human Replication Complex" * |
| 郑虹宁: "基于DNA环状小分子的DNA和RNA纳米组装技术" * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116287124A (zh) * | 2023-05-24 | 2023-06-23 | 中国农业科学院农业基因组研究所 | 单链接头预连接方法、高通量测序文库的建库方法及试剂盒 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP3789490A1 (en) | 2021-03-10 |
| US20210024933A1 (en) | 2021-01-28 |
| WO2019205135A1 (zh) | 2019-10-31 |
| CN111742048B (zh) | 2024-02-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111742048A (zh) | 用于对单链核酸分子进行环化处理的夹板核酸分子及其应用 | |
| EP3464634B1 (en) | Molecular tagging methods and sequencing libraries | |
| WO2017215500A1 (zh) | 一种核酸等温自扩增方法 | |
| US5354668A (en) | Methods for the isothermal amplification of nucleic acid molecules | |
| CN112795620A (zh) | 双链核酸环化方法、甲基化测序文库构建方法和试剂盒 | |
| WO2013123220A1 (en) | Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions | |
| CN104212791B (zh) | 一种基于双向等温延伸的核酸合成方法 | |
| CN107893109B (zh) | 一种基于移除野生型序列的低丰度基因突变富集方法 | |
| TW201718875A (zh) | 擴增ｄｎａ的方法 | |
| CN113249437A (zh) | 一种用于sRNA测序的建库方法 | |
| WO2023098492A1 (zh) | 测序文库构建方法及应用 | |
| CN112410331A (zh) | 带分子标签和样本标签的接头及其单链建库方法 | |
| WO2021051378A1 (zh) | 测序文库的构建方法、测序方法及试剂盒和应用 | |
| CN114032288A (zh) | 一种试剂盒及其制备测序用靶核苷酸的方法 | |
| CN107083427B (zh) | Dna连接酶介导的dna扩增技术 | |
| CN107858401A (zh) | 一种低丰度基因突变的富集方法 | |
| CN111826421A (zh) | 一种pcr随机引物和使用其构建靶向测序文库的方法 | |
| WO2020252720A1 (zh) | 基于rna样本构建文库的方法及应用 | |
| EP3802872A1 (en) | Cleavable co-operative primers and method of amplifying nucleic acid sequences using same | |
| CN116004773A (zh) | 一种线性置换等温扩增方法及其应用 | |
| JP4962899B2 (ja) | 環状１本鎖ｄｎａの製造方法 | |
| WO2019166784A1 (en) | Methods and compositions for enriching nucleic acids | |
| CN114507904B (zh) | 一种制备二代测序文库的方法 | |
| WO2024092562A1 (zh) | 一种封闭序列及其试剂盒和使用方法 | |
| WO2020259455A1 (zh) | 一种构建PacBio测序文库的方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |