CN111732206A - Lake and river ecological restoration agent - Google Patents
Lake and river ecological restoration agent Download PDFInfo
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- CN111732206A CN111732206A CN202010622726.4A CN202010622726A CN111732206A CN 111732206 A CN111732206 A CN 111732206A CN 202010622726 A CN202010622726 A CN 202010622726A CN 111732206 A CN111732206 A CN 111732206A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/163—Nitrates
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/02—Odour removal or prevention of malodour
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A20/00—Water conservation; Efficient water supply; Efficient water use
- Y02A20/40—Protecting water resources
- Y02A20/402—River restoration
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Abstract
The invention discloses a lake and river channel ecological restoration agent, which belongs to the technical field of ecological environment restoration and comprises the following substances: nitrifying bacteria, denitrifying bacteria, lactic acid bacteria, yeasts, photosynthetic bacteria, bacillus subtilis, actinomycetes, sulfurous bacteria, bifidobacteria, hydrocarbon-philic bacteria, neutral citric acid bacteria and a loading agent. The repairing agent can effectively change the water environment, reduce the turbidity of the water body, convert and degrade pollutants in the water body, reduce the content of the pollutants and provide good environmental conditions for the survival of other organisms.
Description
Technical Field
The invention belongs to the technical field of ecological environment restoration, and particularly relates to a lake and river channel ecological restoration agent.
Background
At present, the problems of ecological lakes and river waters in China mainly include eutrophication of water bodies, a large amount of nutrients such as nitrogen and phosphorus flowing into slow-flow water bodies such as lakes, river mouths and gulfs, which causes rapid propagation of algae and other plankton, decrease of dissolved oxygen in water bodies, deterioration of water quality, death of fishes and other organisms, and formation of bloom due to mass propagation of plankton when eutrophication of water bodies occurs, and water surfaces often show blue, red, brown, milky white and the like and emit unpleasant odor due to different colors of dominant plankton, and river degeneration is recognized as a global ecological environment problem, which is generally concerned by international society, and as environmental awareness, ecological concept enhancement and living level improvement, social requirements for repairing seriously damaged river ecosystems become more urgent, it is necessary to develop ecological restoration measures for lakes and rivers.
The existing repair treatment is mostly the repair treatment of biological agents, for example, the application numbers are as follows: 201510014934.5 discloses an ecological restoration agent for lakes and rivers, wherein a plurality of prepared bacterial liquids achieve good restoration effect. However, when the pollution of sewage and the like is serious, the repairing effect of the strains is influenced to a certain extent, so that the repairing effect is reduced, and the repairing performance is unstable.
Disclosure of Invention
The invention aims to provide a lake and river channel ecological restoration agent which can well restore the ecological environment of polluted lakes and rivers.
The technical purpose of the invention is realized by the following technical scheme:
a lake and river ecological restoration agent comprises the following substances in parts by weight:
25-30 parts of nitrifying bacteria, 15-20 parts of denitrifying bacteria, 20-25 parts of lactic acid bacteria, 10-16 parts of microzyme, 3-6 parts of photosynthetic bacteria, 10-15 parts of bacillus subtilis, 4-6 parts of actinomycetes, 2-6 parts of vulcanized bacteria, 5-8 parts of bifidobacteria, 1-5 parts of hydrocarbon-philic bacteria, 6-10 parts of neutral citric acid bacteria and 4-7 parts of loading agent; the nitrifying bacteria, the denitrifying bacteria, the lactic acid bacteria, the saccharomycetes, the photosynthetic bacteria, the bacillus subtilis, the actinomycetes, the vulcanized bacteria, the bifidobacteria, the hydrocarbon-philic bacteria and the neutral citric acid bacteria are respectively cultured in respective culture media to correspondingly form nitrifying bacteria liquid, denitrifying bacteria liquid, lactic acid bacteria liquid, yeast liquid, photosynthetic bacteria liquid, bacillus subtilis liquid, actinomycetes liquid, vulcanizing bacteria liquid, bifidobacterium liquid, hydrocarbon-philic bacteria liquid and neutral citric acid bacteria liquid; and salt stress treatment is carried out in the cultivation process; the carrier is modified bentonite.
Preferably, the composition comprises the following substances in parts by weight:
28 parts of nitrobacteria, 17 parts of denitrifying bacteria, 23 parts of lactic acid bacteria, 13 parts of yeast, 5 parts of photosynthetic bacteria, 14 parts of bacillus subtilis, 5 parts of actinomycetes, 5 parts of vulcanized bacteria, 7 parts of bifidobacterium, 3 parts of hydrocarbon-philic bacteria, 8 parts of neutral citric acid bacteria and 6 parts of loading agent; the nitrifying bacteria, the denitrifying bacteria, the lactic acid bacteria, the saccharomycetes, the photosynthetic bacteria, the bacillus subtilis, the actinomycetes, the vulcanized bacteria, the bifidobacteria, the hydrocarbon-philic bacteria and the neutral citric acid bacteria are respectively cultured in respective culture media to correspondingly form nitrifying bacteria liquid, denitrifying bacteria liquid, lactic acid bacteria liquid, yeast liquid, photosynthetic bacteria liquid, bacillus subtilis liquid, actinomycetes liquid, vulcanizing bacteria liquid, bifidobacterium liquid, hydrocarbon-philic bacteria liquid and neutral citric acid bacteria liquid; and salt stress treatment is carried out in the cultivation process; the carrier is modified bentonite.
Further, the salt stress treatment is specifically performed by: in the cultivation process, salt accounting for 1.5-1.8% of the total mass of the culture medium is added into the culture medium, and cultivation treatment is carried out after the mixture is uniformly mixed.
Further, the preparation method of the loading agent comprises the following steps:
(1) firstly, putting bentonite into a muffle furnace for calcination treatment, and taking out the bentonite for later use after 1-1.5 h;
(2) immersing the bentonite treated in the step (1) into a silane coupling agent, stirring at a high speed for 35-40 min, and filtering for later use;
(3) putting the bentonite treated in the step (2) into the composite modified liquid, carrying out ultrasonic treatment for 40-50 min, and filtering out for later use;
(4) and (4) putting the bentonite treated in the step (3) into a drying box for drying treatment until the integral water content is not more than 7%, and taking out to obtain the modified bentonite.
Further, the temperature in the muffle furnace is controlled to be 800-850 ℃ during the calcination treatment in the step (1).
Further, the rotation speed of stirring is controlled to be 2500-2800 rpm during the high-speed stirring treatment in the step (2).
Further, the composite modification liquid in the step (3) comprises the following components in parts by weight: 25-30 parts of nano titanium dioxide, 10-15 parts of hexadecyl trimethyl ammonium bromide, 8-12 parts of disodium ethylene diamine tetraacetate, 5-8 parts of sodium pyrophosphate, 4-7 parts of sodium carboxymethyl cellulose and 300-350 parts of deionized water; and controlling the frequency of ultrasonic waves to be 600-700 kHz during ultrasonic treatment.
Further, the temperature in the drying box is controlled to be 100-120 ℃ during the drying treatment in the step (4).
Various strains are reasonably prepared in the repairing agent, wherein the nitrifying bacteria can remove C, N, kill viruses, degrade pesticides, flocculate heavy metals and organic debris in water, and can also digest organic sludge when digesting organic pollution such as carbon series, nitrogen series and the like, the denitrifying bacteria can reduce nitrate into NO and N, and molecular nitrogen returns to the atmosphere to change water quality; the lactobacillus can generate amino oxidase and enzymes for decomposing sulfides, can completely oxidize odor source-indole noise compounds into substances which are non-toxic, non-odor and non-pollution, reduces the odor of water bodies, is an oxygen-resistant anaerobic bacterium, can decompose and utilize various organic carbon and nitrogen substances under the anaerobic or facultative anaerobic condition, is an important component microorganism for biological sewage treatment, is heterotrophic beneficial bacterium and can degrade organic matters, can change molecular ammonia nitrogen into ion ammonium which can be utilized by algae by absorbing saccharides generated by photosynthetic bacteria and saccharomycetes and acidic substances such as generated lactic acid and the like, can generate a large amount of lactein,the lactobacillus has a biological removal effect on microcystins generated by blue-green algae such as microcystis aeruginosa, anabaena flos-aquae, nostoc candida and the like in eutrophic water, has strong bactericidal capacity, can effectively inhibit the activity of harmful microorganisms and the rapid putrefaction and decomposition of organic matters, can decompose lignin and cellulose which are not easily decomposed in a normal state, and ferment and decompose the organic matters, can inhibit the proliferation of pathogenic bacteria generated by successive cropping obstacles, is active, can rapidly increase harmful nematodes, can weaken plants, inhibits the pathogenic bacteria, and gradually disappears; the yeast, photosynthetic bacteria, bacillus subtilis, actinomycetes, sulfurous bacteria, bifidobacterium, hydrocarbon-philic bacteria and neutral citric acid bacteria have the function of assimilating nitrate and have the same function as denitrifying bacteria; mixing nitrobacteria, photosynthetic bacteria, sulfureted bacteria and bacillus subtilis, removing animal excrement, animal and plant residues, harmful gases such as ammonia and hydrogen sulfide, etc. accumulated at the bottom of water body for a long time in water environment, decomposing the substances into smaller organic substances such as amino acid, lower fatty acid, monosaccharide, cyclic hydrocarbon, etc. firstly and then decomposing the substances into CO2Nitrate, sulfate, N2The COD and the BOD of the water body are effectively reduced, the ammonia nitrogen and the nitrite nitrogen in the water body are reduced, the transparency is improved, the total phosphorus content is reduced, and the effect of purifying the water quality is achieved; the photosynthetic bacteria can utilize residual organic matters (such as Hs, NH and the like) in water as a donor of hydrogen to carry out photosynthesis, reduce harmful substances in decomposed water, improve water quality and relatively improve the effect of dissolved oxygen, so that the water quality is recovered to be normal; hydrophilics can remove most of oil stains, so that the oil stains are converted into CO and mycoprotein: the bacillus subtilis self-synthesizes enzymes such as alpha-amylase, protease, lipase, cellulase and the like, the bacillus subtilis quickly consumes free oxygen in the environment, promotes the growth of beneficial anaerobic bacteria and generates organic acids such as lactic acid and the like; the secretion of neutral citric acid bacteria has good inhibitory effect on growth of Pinctada galbana, Chlamydomonas reinhardtii, Microcystis aeruginosa, etc., and can be used in combination with lactobacillus for increasing yieldThe effect of purifying water is strong.
The strain is subjected to salt stress treatment in the cultivation process, and the environmental tolerance of the strain and the catalytic capability of a substrate can be improved after the treatment, so that the repairing agent can be better applied to water bodies and environments with different pollution conditions.
In addition, the invention also prepares and uses a loading agent, and carries out modification treatment on the basis of the traditional loading agent, the modified bentonite has stronger specific surface area and adsorption capacity, and can adsorb, fix and remove filterable active phosphorus from eutrophic water body, so that the algae loses nutrients depending on survival, cuts off the nutrient source of the algae, destroys the circulation path of growth and proliferation of the algae, inhibits the growth of the algae, improves the use stability because the nano titanium dioxide component is modified by intercalation, has good sterilization and catalysis functions on the whole, can promote the growth of strains, and improves the activity and repair capacity of the strains.
Compared with the prior art, the invention has the following advantages:
the repairing agent can effectively change the water environment, reduce the turbidity of the water body, convert and degrade pollutants in the water body, reduce the content of the pollutants and provide good environmental conditions for the survival of other organisms; the mass propagation of algae can be effectively inhibited, and the dissolved oxygen content in the water body is improved; the pH value of the water body is quickly adjusted, the environment of the water body is restored, the self-cleaning capacity of the water body is improved, and all indexes in the water body are restored to be in a good and stable state. The repairing agent has wide application range, can effectively repair the seriously polluted water body, has simple use method, is green and safe, and has great popularization and application values and market competitiveness.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A lake and river ecological restoration agent comprises the following substances in parts by weight:
25 parts of nitrobacteria, 15 parts of denitrifying bacteria, 20 parts of lactic acid bacteria, 10 parts of yeast, 3 parts of photosynthetic bacteria, 10 parts of bacillus subtilis, 4 parts of actinomycetes, 2 parts of vulcanized bacteria, 5 parts of bifidobacterium, 1 part of hydrocarbon-philic bacteria, 6 parts of neutral citric acid bacteria and 4 parts of loading agent; the nitrifying bacteria, the denitrifying bacteria, the lactic acid bacteria, the saccharomycetes, the photosynthetic bacteria, the bacillus subtilis, the actinomycetes, the vulcanized bacteria, the bifidobacteria, the hydrocarbon-philic bacteria and the neutral citric acid bacteria are respectively cultured in respective culture media to correspondingly form nitrifying bacteria liquid, denitrifying bacteria liquid, lactic acid bacteria liquid, yeast liquid, photosynthetic bacteria liquid, bacillus subtilis liquid, actinomycetes liquid, vulcanizing bacteria liquid, bifidobacterium liquid, hydrocarbon-philic bacteria liquid and neutral citric acid bacteria liquid; and salt stress treatment is carried out in the cultivation process; the load agent is modified bentonite; the specific operation of the salt stress treatment is as follows: in the cultivation process, 1.5 percent of salt in the total mass is added into the culture medium, and the culture treatment is carried out after the mixture is evenly mixed.
The preparation method of the loading agent comprises the following steps:
(1) firstly, putting bentonite into a muffle furnace for calcination treatment, controlling the temperature in the muffle furnace to be 800 ℃ during calcination treatment, and taking out the bentonite for later use after 1 h;
(2) immersing the bentonite treated in the step (1) into a silane coupling agent, stirring at a high speed of 2500rpm for 35min, and filtering for later use;
(3) putting the bentonite treated in the step (2) into the composite modified liquid, carrying out ultrasonic treatment for 40-50 min, and filtering out for later use; the composite modified liquid comprises the following components in parts by weight: 25 parts of nano titanium dioxide, 10 parts of hexadecyl trimethyl ammonium bromide, 8 parts of ethylene diamine tetraacetic acid, 5 parts of sodium pyrophosphate, 4 parts of sodium carboxymethyl cellulose and 300 parts of deionized water; the frequency of the ultrasonic wave is controlled to be 600kHz during ultrasonic treatment;
(4) and (4) putting the bentonite treated in the step (3) into a drying box for drying treatment, controlling the temperature in the drying box to be 100 ℃ during the drying treatment until the integral water content is not more than 7%, and taking out to obtain the modified bentonite.
Example 2
A lake and river ecological restoration agent comprises the following substances in parts by weight:
25 parts of nitrobacteria, 15 parts of denitrifying bacteria, 23 parts of lactic acid bacteria, 13 parts of yeast, 5 parts of photosynthetic bacteria, 12 parts of bacillus subtilis, 5 parts of actinomycetes, 2 parts of vulcanized bacteria, 5 parts of bifidobacteria, 1 part of hydrocarbon-philic bacteria, 6 parts of neutral citric acid bacteria and 4 parts of loading agent; the nitrifying bacteria, the denitrifying bacteria, the lactic acid bacteria, the saccharomycetes, the photosynthetic bacteria, the bacillus subtilis, the actinomycetes, the vulcanized bacteria, the bifidobacteria, the hydrocarbon-philic bacteria and the neutral citric acid bacteria are respectively cultured in respective culture media to correspondingly form nitrifying bacteria liquid, denitrifying bacteria liquid, lactic acid bacteria liquid, yeast liquid, photosynthetic bacteria liquid, bacillus subtilis liquid, actinomycetes liquid, vulcanizing bacteria liquid, bifidobacterium liquid, hydrocarbon-philic bacteria liquid and neutral citric acid bacteria liquid; and salt stress treatment is carried out in the cultivation process; the load agent is modified bentonite; the specific operation of the salt stress treatment is as follows: in the cultivation process, 1.5 percent of salt in the total mass is added into the culture medium, and the culture treatment is carried out after the mixture is evenly mixed.
The preparation method of the loading agent comprises the following steps:
(1) firstly, putting bentonite into a muffle furnace for calcination treatment, controlling the temperature in the muffle furnace to be 800 ℃ during calcination treatment, and taking out the bentonite for later use after 1.3 h;
(2) immersing the bentonite treated in the step (1) into a silane coupling agent, stirring at a high speed of 2500rpm for 38min, and filtering for later use;
(3) putting the bentonite treated in the step (2) into the composite modified liquid, performing ultrasonic treatment for 40min, and filtering out for later use; the composite modified liquid comprises the following components in parts by weight: 28 parts of nano titanium dioxide, 13 parts of hexadecyl trimethyl ammonium bromide, 10 parts of ethylene diamine tetraacetic acid, 5 parts of sodium pyrophosphate, 4 parts of sodium carboxymethyl cellulose and 300 parts of deionized water; the frequency of the ultrasonic wave is controlled to be 600kHz during ultrasonic treatment;
(4) and (4) putting the bentonite treated in the step (3) into a drying box for drying treatment, controlling the temperature in the drying box to be 100 ℃ during the drying treatment until the integral water content is not more than 7%, and taking out to obtain the modified bentonite.
Example 3
A lake and river ecological restoration agent comprises the following substances in parts by weight:
28 parts of nitrobacteria, 17 parts of denitrifying bacteria, 23 parts of lactic acid bacteria, 13 parts of yeast, 5 parts of photosynthetic bacteria, 14 parts of bacillus subtilis, 5 parts of actinomycetes, 5 parts of vulcanized bacteria, 7 parts of bifidobacterium, 3 parts of hydrocarbon-philic bacteria, 8 parts of neutral citric acid bacteria and 6 parts of loading agent; the nitrifying bacteria, the denitrifying bacteria, the lactic acid bacteria, the saccharomycetes, the photosynthetic bacteria, the bacillus subtilis, the actinomycetes, the vulcanized bacteria, the bifidobacteria, the hydrocarbon-philic bacteria and the neutral citric acid bacteria are respectively cultured in respective culture media to correspondingly form nitrifying bacteria liquid, denitrifying bacteria liquid, lactic acid bacteria liquid, yeast liquid, photosynthetic bacteria liquid, bacillus subtilis liquid, actinomycetes liquid, vulcanizing bacteria liquid, bifidobacterium liquid, hydrocarbon-philic bacteria liquid and neutral citric acid bacteria liquid; and salt stress treatment is carried out in the cultivation process; the specific operation of the salt stress treatment is as follows: in the cultivation process, salt accounting for 1.7 percent of the total mass of the culture medium is added into the culture medium, and cultivation treatment is carried out after the mixture is uniformly mixed.
The preparation method of the loading agent comprises the following steps:
(1) firstly, putting bentonite into a muffle furnace for calcination treatment, controlling the temperature in the muffle furnace to be 830 ℃ during calcination treatment, and taking out the bentonite for later use after 1.3 h;
(2) immersing the bentonite treated in the step (1) into a silane coupling agent, stirring at a high speed of 2700rpm for 38min, and filtering for later use;
(3) putting the bentonite treated in the step (2) into the composite modified liquid, performing ultrasonic treatment for 45min, and filtering out for later use; the composite modified liquid comprises the following components in parts by weight: 28 parts of nano titanium dioxide, 13 parts of hexadecyl trimethyl ammonium bromide, 10 parts of ethylene diamine tetraacetic acid, 7 parts of sodium pyrophosphate, 6 parts of sodium carboxymethyl cellulose and 330 parts of deionized water; the frequency of the ultrasonic wave is controlled to be 660kHz during ultrasonic treatment;
(4) and (4) putting the bentonite treated in the step (3) into a drying box for drying treatment, controlling the temperature in the drying box to be 110 ℃ during the drying treatment until the integral water content is not more than 7%, and taking out the bentonite to obtain the modified bentonite.
Example 4
A lake and river ecological restoration agent comprises the following substances in parts by weight:
28 parts of nitrobacteria, 20 parts of denitrifying bacteria, 25 parts of lactic acid bacteria, 14 parts of yeast, 5 parts of photosynthetic bacteria, 14 parts of bacillus subtilis, 5 parts of actinomycetes, 6 parts of vulcanized bacteria, 8 parts of bifidobacterium, 5 parts of hydrocarbon-philic bacteria, 10 parts of neutral citric acid bacteria and 7 parts of loading agent; the nitrifying bacteria, the denitrifying bacteria, the lactic acid bacteria, the saccharomycetes, the photosynthetic bacteria, the bacillus subtilis, the actinomycetes, the vulcanized bacteria, the bifidobacteria, the hydrocarbon-philic bacteria and the neutral citric acid bacteria are respectively cultured in respective culture media to correspondingly form nitrifying bacteria liquid, denitrifying bacteria liquid, lactic acid bacteria liquid, yeast liquid, photosynthetic bacteria liquid, bacillus subtilis liquid, actinomycetes liquid, vulcanizing bacteria liquid, bifidobacterium liquid, hydrocarbon-philic bacteria liquid and neutral citric acid bacteria liquid; and salt stress treatment is carried out in the cultivation process; the load agent is modified bentonite; the specific operation of the salt stress treatment is as follows: in the cultivation process, salt accounting for 1.7 percent of the total mass of the culture medium is added into the culture medium, and cultivation treatment is carried out after the mixture is uniformly mixed.
The preparation method of the loading agent comprises the following steps:
(1) firstly, putting bentonite into a muffle furnace for calcination treatment, controlling the temperature in the muffle furnace to be 850 ℃ during calcination treatment, and taking out the bentonite for later use after 1.3 h;
(2) immersing the bentonite treated in the step (1) into a silane coupling agent, stirring at a high speed of 2700rpm for 40min, and filtering for later use;
(3) putting the bentonite treated in the step (2) into the composite modified liquid, carrying out ultrasonic treatment for 50min, and filtering out for later use; the composite modified liquid comprises the following components in parts by weight: 30 parts of nano titanium dioxide, 14 parts of hexadecyl trimethyl ammonium bromide, 11 parts of ethylene diamine tetraacetic acid, 8 parts of sodium pyrophosphate, 7 parts of sodium carboxymethyl cellulose and 340 parts of deionized water; controlling the frequency of ultrasonic waves to be 670kHz during ultrasonic treatment;
(4) and (4) putting the bentonite treated in the step (3) into a drying box for drying treatment, controlling the temperature in the drying box to be 115 ℃ during the drying treatment until the integral water content is not more than 7%, and taking out to obtain the modified bentonite.
Example 5
A lake and river ecological restoration agent comprises the following substances in parts by weight:
30 parts of nitrobacteria, 20 parts of denitrifying bacteria, 25 parts of lactic acid bacteria, 16 parts of yeast, 6 parts of photosynthetic bacteria, 15 parts of bacillus subtilis, 6 parts of actinomycetes, 6 parts of vulcanized bacteria, 8 parts of bifidobacterium, 5 parts of hydrocarbon-philic bacteria, 10 parts of neutral citric acid bacteria and 7 parts of loading agent; the nitrifying bacteria, the denitrifying bacteria, the lactic acid bacteria, the saccharomycetes, the photosynthetic bacteria, the bacillus subtilis, the actinomycetes, the vulcanized bacteria, the bifidobacteria, the hydrocarbon-philic bacteria and the neutral citric acid bacteria are respectively cultured in respective culture media to correspondingly form nitrifying bacteria liquid, denitrifying bacteria liquid, lactic acid bacteria liquid, yeast liquid, photosynthetic bacteria liquid, bacillus subtilis liquid, actinomycetes liquid, vulcanizing bacteria liquid, bifidobacterium liquid, hydrocarbon-philic bacteria liquid and neutral citric acid bacteria liquid; and salt stress treatment is carried out in the cultivation process; the load agent is modified bentonite; the specific operation of the salt stress treatment is as follows: in the cultivation process, salt accounting for 1.8 percent of the total mass of the culture medium is added into the culture medium, and cultivation treatment is carried out after the mixture is uniformly mixed.
The preparation method of the loading agent comprises the following steps:
(1) firstly, putting bentonite into a muffle furnace for calcination treatment, controlling the temperature in the muffle furnace to be 850 ℃ during calcination treatment, and taking out the bentonite for later use after 1.5 h;
(2) immersing the bentonite treated in the step (1) into a silane coupling agent, stirring at a high speed of 2800rpm for 40min, and filtering for later use;
(3) putting the bentonite treated in the step (2) into the composite modified liquid, carrying out ultrasonic treatment for 40-50 min, and filtering out for later use; the composite modified liquid comprises the following components in parts by weight: 30 parts of nano titanium dioxide, 15 parts of hexadecyl trimethyl ammonium bromide, 12 parts of ethylene diamine tetraacetic acid, 8 parts of sodium pyrophosphate, 7 parts of sodium carboxymethyl cellulose and 350 parts of deionized water; the frequency of the ultrasonic wave is controlled to be 700kHz during ultrasonic treatment;
(4) and (4) putting the bentonite treated in the step (3) into a drying box for drying treatment, controlling the temperature in the drying box to be 120 ℃ during the drying treatment until the integral water content is not more than 7%, and taking out the bentonite to obtain the modified bentonite.
Comparative example 1
This comparative example 1 is different from example 3 only in that the sulfurous bacteria, bifidobacteria and hydrocarbon-philic bacteria are omitted, except that the process steps are the same.
Comparative example 2
This comparative example 2 is different from example 3 only in that the salt stress treatment operation is omitted except that the process steps are the same.
Comparative example 3
This comparative example 3 is different from example 3 only in that an equal part by mass of ordinary bentonite is used instead of the modified bentonite, except that the process steps are the same.
Comparative example 4
The application numbers are: 201510014934.5, which is characterized in that the technical scheme of the embodiment 3 is specifically selected.
In order to compare the effects of the invention, the ecological restoration agents prepared in the above example 3 and comparative examples 1 to 4 are subjected to performance tests, in order to facilitate comparison and experiments, water bodies are collected in lakes with the same water quality, and then divided into five parts, and 3 groups of repeated tests are performed on each group corresponding to the above five groups of restoration agents, wherein the specific comparative data are shown in the following table 1:
TABLE 1
As can be seen from the above table 1, the repairing agent of the present invention can effectively repair the water body, and improve the quality of the water body.
In order to further compare the effects of the invention, the ecological restoration agents prepared in the above example 3 and comparative examples 1 to 4 are subjected to performance testing again, for the sake of facilitating comparison and experiments, water bodies are collected in rivers with the same water quality, and then divided into five parts, and 3 groups of repeated tests are performed on each group corresponding to the above five groups of restoration agents, wherein the specific comparative data are shown in the following table 2:
TABLE 2
As can be seen from the above table 2, the repairing agent of the present invention has a good repairing effect on acidic water, and the quality of water is well improved.
Therefore, the ecological restoration agent has stronger use performance, obviously improves the restoration effect on the ecological environment of lakes and rivers, and has great popularization and application values.
Claims (8)
1. The ecological restoration agent for lakes and rivers is characterized by comprising the following substances in parts by weight:
25-30 parts of nitrifying bacteria, 15-20 parts of denitrifying bacteria, 20-25 parts of lactic acid bacteria, 10-16 parts of microzyme, 3-6 parts of photosynthetic bacteria, 10-15 parts of bacillus subtilis, 4-6 parts of actinomycetes, 2-6 parts of vulcanized bacteria, 5-8 parts of bifidobacteria, 1-5 parts of hydrocarbon-philic bacteria, 6-10 parts of neutral citric acid bacteria and 4-7 parts of loading agent; the nitrifying bacteria, the denitrifying bacteria, the lactic acid bacteria, the saccharomycetes, the photosynthetic bacteria, the bacillus subtilis, the actinomycetes, the vulcanized bacteria, the bifidobacteria, the hydrocarbon-philic bacteria and the neutral citric acid bacteria are respectively cultured in respective culture media to correspondingly form nitrifying bacteria liquid, denitrifying bacteria liquid, lactic acid bacteria liquid, yeast liquid, photosynthetic bacteria liquid, bacillus subtilis liquid, actinomycetes liquid, vulcanizing bacteria liquid, bifidobacterium liquid, hydrocarbon-philic bacteria liquid and neutral citric acid bacteria liquid; and salt stress treatment is carried out in the cultivation process; the carrier is modified bentonite.
2. The ecological restoration agent for lakes and rivers according to claim 1, which comprises the following substances in parts by weight:
28 parts of nitrobacteria, 17 parts of denitrifying bacteria, 23 parts of lactic acid bacteria, 13 parts of yeast, 5 parts of photosynthetic bacteria, 14 parts of bacillus subtilis, 5 parts of actinomycetes, 5 parts of vulcanized bacteria, 7 parts of bifidobacterium, 3 parts of hydrocarbon-philic bacteria, 8 parts of neutral citric acid bacteria and 6 parts of loading agent; the nitrifying bacteria, the denitrifying bacteria, the lactic acid bacteria, the saccharomycetes, the photosynthetic bacteria, the bacillus subtilis, the actinomycetes, the vulcanized bacteria, the bifidobacteria, the hydrocarbon-philic bacteria and the neutral citric acid bacteria are respectively cultured in respective culture media to correspondingly form nitrifying bacteria liquid, denitrifying bacteria liquid, lactic acid bacteria liquid, yeast liquid, photosynthetic bacteria liquid, bacillus subtilis liquid, actinomycetes liquid, vulcanizing bacteria liquid, bifidobacterium liquid, hydrocarbon-philic bacteria liquid and neutral citric acid bacteria liquid; and salt stress treatment is carried out in the cultivation process; the carrier is modified bentonite.
3. The ecological restoration agent for lakes and rivers according to claim 1 or 2, wherein the salt stress treatment is performed by the following steps: in the cultivation process, salt accounting for 1.5-1.8% of the total mass of the culture medium is added into the culture medium, and cultivation treatment is carried out after the mixture is uniformly mixed.
4. The ecological restoration agent for lakes and rivers according to claim 1 or 2, wherein the preparation method of the loading agent comprises the following steps:
(1) firstly, putting bentonite into a muffle furnace for calcination treatment, and taking out the bentonite for later use after 1-1.5 h;
(2) immersing the bentonite treated in the step (1) into a silane coupling agent, stirring at a high speed for 35-40 min, and filtering for later use;
(3) putting the bentonite treated in the step (2) into the composite modified liquid, carrying out ultrasonic treatment for 40-50 min, and filtering out for later use;
(4) and (4) putting the bentonite treated in the step (3) into a drying box for drying treatment until the integral water content is not more than 7%, and taking out to obtain the modified bentonite.
5. The ecological restoration agent for lakes and rivers according to claim 4, wherein the temperature in the muffle furnace is controlled to be 800-850 ℃ during the calcination treatment in the step (1).
6. The ecological restoration agent for lakes and rivers according to claim 4, wherein the rotation speed of stirring during the high-speed stirring treatment in the step (2) is controlled to be 2500-2800 rpm.
7. The ecological restoration agent for lakes and rivers according to claim 4, wherein the composite modification liquid in the step (3) comprises the following components in parts by weight: 25-30 parts of nano titanium dioxide, 10-15 parts of hexadecyl trimethyl ammonium bromide, 8-12 parts of disodium ethylene diamine tetraacetate, 5-8 parts of sodium pyrophosphate, 4-7 parts of sodium carboxymethyl cellulose and 300-350 parts of deionized water; and controlling the frequency of ultrasonic waves to be 600-700 kHz during ultrasonic treatment.
8. The ecological restoration agent for lakes and rivers according to claim 4, wherein the temperature in the drying box is controlled to be 100-120 ℃ during the drying treatment in the step (4).
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| CN118360209A (en) * | 2024-05-08 | 2024-07-19 | 青岛宏洋生物科技有限公司 | Composite microbial agent for aquaculture and preparation method and preparation device thereof |
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