CN111728674A - Optical fiber probe human tissue information online monitoring system based on Raman scattering - Google Patents
Optical fiber probe human tissue information online monitoring system based on Raman scattering Download PDFInfo
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- CN111728674A CN111728674A CN202010528770.9A CN202010528770A CN111728674A CN 111728674 A CN111728674 A CN 111728674A CN 202010528770 A CN202010528770 A CN 202010528770A CN 111728674 A CN111728674 A CN 111728674A
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- optical fiber
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/34—Trocars; Puncturing needles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/02—Instruments for taking cell samples or for biopsy
- A61B10/0233—Pointed or sharp biopsy instruments
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/34—Trocars; Puncturing needles
- A61B17/3417—Details of tips or shafts, e.g. grooves, expandable, bendable; Multiple coaxial sliding cannulas, e.g. for dilating
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0075—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
- A61B5/0084—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters
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- Spectroscopy & Molecular Physics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention relates to the technical field of medical instruments, in particular to an optical fiber probe human tissue information online monitoring system based on Raman scattering, which comprises a power supply, a laser light source, an optical circulator, a puncture needle, a Raman spectrum analyzer and a computer, wherein the power supply is electrically connected with the laser light source, the laser light source is connected with the optical circulator through a laser optical fiber, the optical circulator is respectively connected with the puncture needle and the Raman spectrum analyzer through the laser optical fiber and a collection optical fiber, the Raman spectrum analyzer is connected with the computer, and the optical fiber probes are distributed and arranged on the outer side edge of a needle core of the puncture needle. The system combines the Raman spectrum analysis technology with the traditional method for extracting the human tissue by puncturing, solves the problem that cancerous tissues and normal tissues cannot be identified in the current operation, thoroughly changes the condition that a surgeon makes medical judgment by means of imaging examination and personal experience before the operation of a patient, effectively reduces the damage to healthy human tissues and reduces the pain of the patient.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to an optical fiber probe human tissue information online monitoring system based on Raman scattering.
Background
At present, with the development of communication technology and laser technology, the optical fiber sensor is combined with the spectrum technology, so that the application field of the raman spectrum monitoring technology is more extensive. Raman spectroscopy is also of increasing value in medical diagnostics due to its unique advantages, such as non-contact, non-destructive, and "fingerprint" resolution. Biochemical changes in cells or tissues that may cause disease can result in significant changes in raman spectra that can detect such biochemical changes at the molecular level. Especially in the aspect of cancer treatment, different substance structures are identified and distinguished by analyzing and capturing Raman characteristic spectra, so that the difference between a cancer cell tissue structure and a normal cell tissue structure can be revealed, and the difference between the Raman spectra of a certain number of cancerous and normal organ tissues is found out through comparison of the Raman spectra, so that disease diagnosis is realized.
Needle biopsy is the primary way to obtain histopathological specimens and is the most common method for early diagnosis of cancer. However, the main problems faced by the current clinical needle biopsy are that the tissue extraction amount is small, the sampling accuracy is not high, the cancerous tissue and the normal tissue cannot be identified in real time, the boundary between the cancerous tissue and the normal tissue is difficult to be accurately distinguished only by the experience of a doctor and the observation with naked eyes, the accuracy of the biopsy is restricted, the confirmed diagnosis can be obtained by multiple times of biopsy, the pain and the puncture risk of a patient are increased, and the operation steps of the doctor are increased.
In view of the defects of the prior art for extracting human tissues by a puncture needle, the invention provides a puncture biopsy technology combining Raman spectrum and an optical fiber probe, which can improve the puncture safety, accuracy and stability as much as possible.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides an optical fiber probe human tissue information online monitoring system based on Raman scattering, which effectively overcomes the defects of low biopsy accuracy and insufficient biopsy stability of the existing needle biopsy technology.
In order to achieve the purpose, the technical scheme applied by the invention is as follows:
the utility model provides an online monitoring system of fiber probe human tissue information based on raman scattering, includes power, laser light source, light circulator, pjncture needle, raman spectrum appearance and computer, and the power electricity is connected in laser light source, and laser light source passes through laser fiber and connects in light circulator, and light circulator passes through laser fiber and collects optic fibre and connects respectively in pjncture needle and raman spectrum appearance, and raman spectrum appearance connects in the computer, and the needle core outside edge distribution of pjncture needle has arranged fiber probe.
According to the scheme, the puncture needle is a puncture biopsy needle.
According to the scheme, the optical fiber probe consists of a conical nanometer optical fiber probe.
According to the scheme, one side of the puncture needle can be fixed by adopting an adhesive strip adhered to the skin.
According to the scheme, the Raman spectrum analyzer is a dispersion type Raman spectrum analyzer.
According to the scheme, the output wavelength of the laser light source is 532nm, and the output power is stabilized at 0-500 nm.
The invention has the beneficial effects that:
1) the optical fiber probe human tissue information online monitoring system based on Raman scattering adopts a novel method for extracting human tissue by using the puncture biopsy needle with the tapered optical fiber probe, and the used optical fiber probe has the advantages of non-contact property, high sensitivity, strong electromagnetic interference resistance, electrical insulation, elimination of the problem of isolation from the ground and the like, so that the extraction process is more accurate, rapid, safe and effective;
2) the system combines the Raman spectrum analysis technology with the traditional method for extracting the human tissue by puncturing, solves the problem that the cancerous tissue and the normal tissue cannot be identified in the current operation, thoroughly changes the condition that a surgeon makes medical judgment by means of the imaging examination and personal experience before the operation of a patient, effectively reduces the damage to the healthy human tissue and reduces the pain of the patient;
3) in the system, the puncture needle can be fixed on the skin by adopting an adhesive strip, so that the fiber probe can be more stably detected during puncture biopsy, the working efficiency is improved, and the working intensity of doctors is reduced.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
fig. 2 is a schematic view of the structure of the puncture needle of the present invention.
1. A power source; 2. a laser light source; 3. an optical circulator; 4. a needle biopsy needle; 5. a Raman spectrum analyzer; 6. a computer; 7. sticking the connecting strips; 8. a fiber optic probe.
Detailed Description
The technical solution of the present invention is described below with reference to the accompanying drawings and examples.
As shown in fig. 1 and 2, the system for monitoring human tissue information on line by using a fiber-optic probe based on raman scattering comprises a power supply 1, a laser light source 2, an optical circulator 3, a puncture needle 4, a raman spectrum analyzer 5 and a computer 6, wherein the power supply 1 is electrically connected with the laser light source 2, the laser light source 2 is connected with the optical circulator 3 through the laser fiber, the optical circulator 3 is respectively connected with the puncture needle 4 and the raman spectrum analyzer 5 through the laser fiber and a collection fiber, the raman spectrum analyzer 5 is connected with the computer 6, and fiber-optic probes 8 are distributed and arranged on the outer edge of a needle core of the puncture needle 4. The above constitutes the basic structure of the present invention.
The invention adopts the structure arrangement, and the working principle is as follows: the laser light source 2 is transmitted to the tip of the puncture needle 4 through the optical circulator 3 and the optical fiber probe 8, so that Raman spectrum signals of tissues or cells contacted with the tip of the puncture needle are obtained, and real-time capture, monitoring and automatic analysis are realized; the Raman spectrum analyzer 5 can analyze and monitor the Raman spectrum signals of the tissues or cells at the needle point in real time, so as to realize the identification of the tissues at the needle point position, and the analyzed tissues are displayed on a computer. The system combines the Raman spectrum analysis technology with the traditional method for extracting the human tissue by puncturing, solves the problem that the cancerous tissue and the normal tissue cannot be identified in the current operation, thoroughly changes the condition that a surgeon makes medical judgment by means of the imaging examination and personal experience before the operation of a patient, effectively reduces the damage to the healthy human tissue, and reduces the pain of the patient
In practical application, the optical circulator 3 can transmit an input optical signal from the laser fiber to the puncture needle 4 through the laser light source 2, and output the acquired raman signal to the raman spectrum analyzer 5 through the collection fiber for processing and analysis.
In practical application, the computer 6 can display the raman spectrum signal after being compared by the raman spectrometer analyzer 5 in real time, and process the signal, thereby more accurately and rapidly helping doctors to distinguish whether the tissue is cancerated tissue or normal tissue.
In the present embodiment, the puncture needle 4 is a biopsy needle.
In this embodiment, the optical fiber probe 8 is composed of a tapered nano optical fiber probe, and is distributed and arranged at the outer edge of the needle core of the puncture needle 4. The method has the advantages of non-contact property, high sensitivity, strong anti-electromagnetic interference, electric insulation, elimination of the problem of isolation from the ground and the like, and can enable the extraction process to be more accurate, rapid, safe, effective and rapid to obtain the spectral information of the human tissue.
In this embodiment, one side of the needle 4 can be fixed by adhesion to the skin using an adhesive strip 7. The fiber probe 8 can be used for more stable detection, so that the needle biopsy result is more feasible and effective. The problem of inaccurate detection caused by displacement of the optical fiber probe 8 is reduced. Improve work efficiency and reduce the working strength of doctors.
In this embodiment, the raman spectrometer 5 is a dispersive raman spectrometer. The Raman spectrum analyzer 5 is used for analyzing the monitored Raman spectrum signals of the tissues or cells at the needle tip of the puncture needle 4 in real time.
In this embodiment, the output wavelength of the laser light source 2 is 532nm, and the output power is stabilized at 0-500 nm.
The working process of the invention is as follows:
the power supply 1 is connected with the laser light source 2 to generate 532nm laser, and the laser is transmitted to the tissue at the tip of the needle core of the puncture needle 4 through the optical circulator 3 and the transmission optical fiber to obtain a Raman spectrum signal of the current position; the outlet end of an optical loop of the optical circulator 3 is connected with the inlet end of a Raman spectrum analyzer 5, and the outlet end of the Raman spectrum analyzer 5 is connected with a computer 6; the laser irradiates human tissue, the generated optical information reaches the optical circulator 3 through the nano optical fiber probe 8, reaches the Raman spectrum analyzer 5 through the optical circulator 3, the spectrum is analyzed by the computer 6 to judge whether the tissue is cancerated tissue or normal tissue, and the analysis result is displayed by the computer 6.
While the embodiments of the present invention have been described, the present invention is not limited to the above-mentioned embodiments, which are only illustrative and not restrictive, and those skilled in the art can make various modifications without departing from the spirit and scope of the present invention as defined by the appended claims.
Claims (6)
1. The utility model provides an online monitoring system of fiber probe human tissue information based on raman scattering which characterized in that: including power (1), laser light source (2), light circulator (3), pjncture needle (4), raman spectrum analysis appearance (5) and computer (6), power (1) electricity is connected in laser light source (2), laser light source (2) are connected in light circulator (3) through laser fiber, light circulator (3) are connected respectively in pjncture needle (4) and raman spectrum analysis appearance (5) through laser fiber and collection optic fibre, raman spectrum analysis appearance (5) are connected in computer (6), the needle core outside edge distribution arrangement of pjncture needle (4) has fiber probe (8).
2. The on-line monitoring system for human tissue information based on the fiber-optic probe of Raman scattering of claim 1, characterized in that: the puncture needle (4) is a puncture biopsy needle.
3. The on-line monitoring system for human tissue information based on the fiber-optic probe of Raman scattering of claim 1, characterized in that: the optical fiber probe (8) is composed of a conical nanometer optical fiber probe.
4. The axial impact test device for hull deck and side opening structures of claim 1, wherein: one side of the puncture needle (4) can be fixed by adopting an adhesion strip (7) to adhere to the skin.
5. The on-line monitoring system for human tissue information based on the fiber-optic probe of Raman scattering of claim 1, characterized in that: the Raman spectrum analyzer (5) is a dispersion type Raman spectrum analyzer.
6. The on-line monitoring system for human tissue information based on the fiber-optic probe of Raman scattering of claim 1, characterized in that: the output wavelength of the laser light source (2) is 532nm, and the output power is stabilized at 0-500 nm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010528770.9A CN111728674A (en) | 2020-06-11 | 2020-06-11 | Optical fiber probe human tissue information online monitoring system based on Raman scattering |
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| CN202010528770.9A CN111728674A (en) | 2020-06-11 | 2020-06-11 | Optical fiber probe human tissue information online monitoring system based on Raman scattering |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114601506A (en) * | 2022-03-30 | 2022-06-10 | 北京中研智创新技术研究院有限公司 | Brain tissue detection system based on artificial intelligence recognition and Raman spectrum |
| WO2022173404A3 (en) * | 2021-02-11 | 2022-11-24 | Izmir Biyotip Ve Genom Merkezi | Negative pressure based imaging and therapeutic apparatus and system for well-confined abnormal mucosal tissue ablation and working method of the system |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106420041A (en) * | 2015-08-13 | 2017-02-22 | 复旦大学附属肿瘤医院 | High frequency electric operating knife with fiber probe |
| CN106691549A (en) * | 2015-08-13 | 2017-05-24 | 复旦大学附属肿瘤医院 | Puncture biopsy needle with optical fiber probes |
| CN106885798A (en) * | 2017-03-28 | 2017-06-23 | 戎创前沿科技(北京)有限公司 | A kind of Raman detection system |
-
2020
- 2020-06-11 CN CN202010528770.9A patent/CN111728674A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106420041A (en) * | 2015-08-13 | 2017-02-22 | 复旦大学附属肿瘤医院 | High frequency electric operating knife with fiber probe |
| CN106691549A (en) * | 2015-08-13 | 2017-05-24 | 复旦大学附属肿瘤医院 | Puncture biopsy needle with optical fiber probes |
| CN106885798A (en) * | 2017-03-28 | 2017-06-23 | 戎创前沿科技(北京)有限公司 | A kind of Raman detection system |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022173404A3 (en) * | 2021-02-11 | 2022-11-24 | Izmir Biyotip Ve Genom Merkezi | Negative pressure based imaging and therapeutic apparatus and system for well-confined abnormal mucosal tissue ablation and working method of the system |
| CN114601506A (en) * | 2022-03-30 | 2022-06-10 | 北京中研智创新技术研究院有限公司 | Brain tissue detection system based on artificial intelligence recognition and Raman spectrum |
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