CN101716069A - Human body oxidative stress non-invasive fluorescence detection device and method - Google Patents
Human body oxidative stress non-invasive fluorescence detection device and method Download PDFInfo
- Publication number
- CN101716069A CN101716069A CN200910185525A CN200910185525A CN101716069A CN 101716069 A CN101716069 A CN 101716069A CN 200910185525 A CN200910185525 A CN 200910185525A CN 200910185525 A CN200910185525 A CN 200910185525A CN 101716069 A CN101716069 A CN 101716069A
- Authority
- CN
- China
- Prior art keywords
- oxidative stress
- human body
- light
- skin
- detection device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses human body oxidative stress non-invasive fluorescence detection device and method. An excitation light source system comprises one near ultraviolet light source and one light filter and one beam splitter which are arranged on the emergent light path of near ultraviolet light source; wherein the beam splitter splits the emergent light into a reference light and an exciting light. A spectrum acquisition system comprises a conduction optical fiber, a fiber probe and a spectrometer. The conduction optical fiber is used for transmitting the reference light to the spectrometer; the fiber probe is used for transmitting the exciting light to skin part of human body to be tested, collecting fluorescent light generated by excitation on skin of human body and transmitting the fluorescent light to the spectrometer. The spectrometer is used for analyzing and processing the collected spectrum signal and finally obtains oxidative stress status of the detected object. Autofluorescence of skin is utilized to reflect human body oxidative stress status, detection speed is rapid, and result can be displayed in real time. The invention can provide reliable basis for prevention, diagnose and treatment effect evaluation on critical illness.
Description
Technical field
The present invention relates to a kind of human body noinvasive detection method and device, particularly a kind of method and device of using-system fluoroscopic examination human body oxidative stress state.
Background technology
Oxidative stress is meant high activity molecule such as reactive oxygen species (ROS) and the too much or elimination minimizing of active nitrogen bunch (RNS) generation in the body, thereby causes tissue injury.Can produce a small amount of ROS at the normal condition lower body and participate in homergy, free radical is removed in the interior existence of body simultaneously, suppresses the system of radical reaction, makes too much free radical be eliminated or make free radical to reduce, the generation of free radical and removing maintenance balance; But under some pathological state, this mechanism is destroyed, interior free yl significantly increases, too much free radical can directly act on body, cause body injury, simultaneously, body anti-oxidative defense ability drop, oxidability significantly surpasses oxidation resistance and oxidative stress takes place, in the body generation of oxidative stress be exactly come from that free-radical generating increases and (or) the anti-oxidative defense functional lesion.
Oxidative stress is considered to cause the major reason of diabetes and diseases such as chronic complicating diseases, pulmonary fibrosis, epilepsy, hypertension, flu, atherosclerosis and corresponding cardiovascular disease, cancer, nephropathy change and parkinson disease thereof.
Diabetes be with defect of insulin secretion and (or) a group of causing of insulin resistant is the metabolic disease of feature with the chronic hyperglycemia.More and more studies show that, the generation of oxidative stress and diabetes and complication thereof, the development in close relations.On the one hand, diabetics can produce a large amount of free radicals because of glucose and glycosylated protein autoxidation, and a large amount of free radicals make tissue produce oxidative stress or oxidative stress enhancing if untimely removing then can accumulate in tissue.In human body, in the external and zoopery, find that all diabetes cause the enhanced evidence of oxidative stress.On the other hand, oxidative stress enhancing meeting exerts an influence to diabetes: oxidative stress strengthens and can and reduce the sensitivity of peripheral tissues to insulin by the damage beta Cell of islet, causes the generation of diabetes; ROS can the coup injury beta Cell of islet, can also be by influence the synthetic and excretory signal transduction pathway indirect injury beta Cell of islet of insulin; ROS can be used as the signaling molecule that is similar to the 2nd courier and activates many oxidoreduction sensitivity signal paths, these paths comprise NF-kB, P38 mitogen activated protein kinase, JNK, hexosamine etc., and then cause Insulin receptor INSR and IRS protein phosphorylation in the insulin signaling pathway, cause the dependency and/or active reduction of insulin signaling pathway downstream signaling molecule, reduce the effect of insulin, caused insulin resistant; Oxidative stress also can cause insulin resistant by weakening the expression and the transhipment of 3T3-L1 adipose cell glucose transporter 4.In addition, studies confirm that it is one of main pathogeny of chronic complicating diseases of diabetes that oxidative stress strengthens.
The diabetics body contain a large amount of terminal glycosylation dead end products (Advanced glycation endproducts, AGE).AGE is under non-enzyme condition, the one group of stable dead end product that produces behind the free amine group of macromolecular substances such as protein, aminoacid, lipid or nucleic acid and the condensation of the aldehyde radical of reducing sugar process, rearrangement, cracking, the oxidative modification.Behind AGE and its acceptor interaction, produce a large amount of active oxygens, destroy the balance of vivo oxidation agent and antioxidant, thereby produce oxidative stress.Therefore, human body oxidative stress and intravital terminal glycosylation dead end product are closely related.
Although the high oxidation stress level is fast to the damage of body, cause the clinical disease performance of body also to need the regular hour, that is to say to have one " incubation period ".Therefore, can adopt someway, the human body oxidative stress indicator reaction is measured, understand the oxidative stress level of human body, thereby prevention and is carried out auxiliary treatment to existing disease reliable foundation is provided because of the disease due to the oxidative stress.
The high performance liquid chromatography of human body oxidative stress mark at first will be carried out sample collection to tested object at present, for example blood drawing, and then deliver to laboratory and carry out assay.This analytical method that wound arranged not only one-shot measurement need expend the plenty of time, measure the cost height, and the blood drawing chemical examination increased the misery of tested object, may have the risk of infection, thereby limit the number of times that detects, and is unfavorable for the early diagnosis of disease.
At present, people have done some trials and effort aspect the human health status utilizing the skin histology autofluorescence to judge.For example, by the tested object part is anaesthetized, obtain the skin biopsy sample, sample carries out freezing preservation usually.Before analyzing, skin samples through thawing processing, excises subcutaneous fat more simultaneously, remainder is organized and also need be cleaned and drying, again sample is carried out fluoroscopic examination, the shortcoming of this method is to cut into slices to measured's skin, and the sample treatment process is loaded down with trivial details consuming time.
International monopoly WO sets forth a kind of device of measuring the skin autofluorescence No. 01/22869.Described device adds optical filter by wideband light source and constitutes, and SF is detected by spectrogrph.The inventor proposes and can but have any method of using described device quantitative measurement AGE be described, and do not proposed the purposes of described device in medical diagnosis on disease by the terminal glycosylation dead end product in the SF detection by quantitative skin.At the interference of individual's skin color and structure, do not propose any compensation yet or proofread and correct the method for fluorescence spectrum fluorescence measurement.
A kind of apparatus and method of using the skin histology autofluorescence to determine a certain glycation end product or morbid state have been set forth in the Chinese invention patent application for 200710003189.X number.Described device is made up of xenon lamp and monochromator, and fluorescence signal is received by photodetector.The inventor has only set forth the autofluorescence of glycation end product and the relation between the diabetes, does not set up the relation between skin histology autofluorescence and the human body oxidative stress.
Summary of the invention
The object of the present invention is to provide a kind of histofluorescence of utilizing, the device and method of real-time, noninvasive human body oxidative stress status.
Human body oxidative stress non-invasive fluorescence detection device includes excitation light source system, spectra collection system and spectral signal analysis process system:
Excitation light source system comprises the near ultraviolet light source, optical filter and beam splitter near ultraviolet light source emitting light path, and beam splitter is divided into reference light and exciting light to emergent light;
The spectra collection system comprises conduction optical fiber, fibre-optical probe and spectrogrph; Conduction optical fiber is used for reference light is transferred to spectrogrph; Fibre-optical probe is used to conduct exciting light to tested human body skin part, collects human body skin because of exciting the fluorescence that produces and transferring to spectrogrph; Spectrogrph is used for the detection of spectral signal;
The spectral signal analysis process system is used for the spectral signal that analyzing and processing collected, and finally obtains the oxidative stress status of measurand.
Described human body oxidative stress non-invasive fluorescence detection device is characterized in that described near ultraviolet light source has the wavelength of 300nm-450nm scope, can be the combination of wideband light source or a plurality of narrow-band light source.
Described human body oxidative stress non-invasive fluorescence detection device is characterized in that described optical filter is the combination of narrow band pass filter or a plurality of cut-off type optical filters.
Described human body oxidative stress non-invasive fluorescence detection device is characterized in that conducting the spectral signal that optical fiber can transmit 300nm-600nm, can be the fibre bundle that optical fiber or multifiber are formed.
Described human body oxidative stress non-invasive fluorescence detection device is characterized in that described fibre-optical probe by conducting optical fiber and collecting optical fiber and form, can be polymorphic fibre bundle.
Described human body oxidative stress non-invasive fluorescence detection device is characterized in that the spectral signal acquisition system gathers the spectral signal through skin histology reflection and scattering simultaneously, determines calibrated fluorescence measurement value by the reflection characteristic of organizing.
Described human body oxidative stress non-invasive fluorescence detection device is characterized in that the biological information of spectral signal analysis process system with tested object, and the model that calibrated fluorescence measurement value and structural state are associated is in conjunction with the state with definite described tissue.
Described human body oxidative stress non-invasive fluorescence detection device, wherein said biological information comprise the melanin and the haemachrome level of individual race, sex, height, body weight, whether smoking, family's medical history, skin.
Described human body oxidative stress non-invasive fluorescence detection device, wherein said model is used for method of least square, covariance analysis method, PCA, multiple regression analysis method, neural network algorithm the mensuration of structural state by the fluorescent characteristic of a plurality of tested object skin histologies and the skin histology state of tested object are detected.
The human body oxidative stress non-invasive fluorescence detection method is characterized in that may further comprise the steps:
The light that the near ultraviolet light source produces, it is identical to form two bundle wavelength through optical filter and beam splitter, and the monochromatic light that the direction of propagation is different is wherein a branch of as exciting light I
1(λ), another bundle conduct is with reference to light I
2(λ); Exciting light transfers to human test position skin by conduction optical fiber, the terminal glycosylation dead end product is stimulated and produces the fluorescence of 420nm-600nm in the skin histology of human test position, fluorescence is by receiving collecting fiber, and the exciting light of skin histology reflection and scattering also is received collecting fiber simultaneously; To spectrogrph, the measured signal note of spectrogrph is made F to the spectrum that reception optical fiber is collected through fiber optic conduction
Meas(λ
x, λ
m); Reference light I
2(λ) be directly conducted to described spectrogrph through optical fiber, spectrogrph transfers to computer after optical signal is converted into the signal of telecommunication.
Reference light is used for the influence of calibration light source fluctuation to measurement result.Skin reflex light and skin scattered light are used to proofread and correct the absorption of tested object skin and the scattering process influence to measurement result, thereby obtain the intrinsic fluorescence spectra of tested object.
The spectral signal analysis process system is to the biological information of tested object, and the model that intrinsic fluorescence spectra and structural state are associated is analyzed, and obtains the oxidative stress status of described tissue.Described biological information comprises the melanin and the haemachrome level of individual race, sex, height, body weight, whether smoking, family's medical history, skin; Described model is by a plurality of tested object skin histology optical characteristics are detected, and utilization method of least square, covariance analysis method, PCA, multiple regression analysis method, neural network algorithm are to determine the skin histology state; Described oxidative stress status comprises: the speed that whether vivo oxidation stress exist, oxidative stress degree and oxidative stress degree change; Has, do not have the probability that oxidative stress takes place; Comprise the variation of terminal glycosylation dead end product concentration, accumulation rate and accumulation rate in the tissue simultaneously.
The present invention has following characteristics and advantage:
1. need not human body draw blood chemical examination or skin biopsy sampling, painful and infected risk that having avoided the blood drawing chemical examination to bring to patient.
2. utilize the autofluorescence reflection human body oxidative stress state of skin, detection speed is fast, shows testing result in real time.
3. measurement result not only reflects the human body oxidative stress state, and can follow the tracks of the generation and the development of diabetes and complication thereof, can provide reliable basis for the evaluation of prevention, diagnosis and the therapeutic effect of major disease.
Description of drawings
Fig. 1 is a kind of human body oxidative stress non-invasive fluorescence detection device enforcement figure.
Fig. 2 is Y shape fibre-optical probe a, three kinds of compound modes of b, c.
(a) fibre-optical probe structural representation among Fig. 3; (b) fibre-optical probe end view drawing.
Fig. 4 is different tested object skin histology autofluorescence primary light spectrograms.
Fig. 5 is that the partial test subject's skin is organized reflectance curve.
Fig. 6 is the measurement result comparison diagram of partial test object.
Fig. 7 is that tested object oxidative stress status cross validation posterior probability box must figure.
Fig. 8 is the relevant experimenter's performance curve of the present invention.
The specific embodiment
The free amine group of macromolecular substances such as human body skin internal protein, aminoacid, lipid or nucleic acid, one group of stable dead end product producing after through condensation, rearrangement, cracking, oxidative modification with the aldehyde radical of reducing sugar under the non-enzyme condition is the terminal glycosylation dead end product.Behind terminal glycosylation dead end product and its acceptor interaction, produce a large amount of active oxygens, destroy the balance of vivo oxidation agent and antioxidant, thereby produce oxidative stress.Therefore, can reflect oxidative stress status by the terminal glycosylation dead end product in the human body skin.The terminal glycosylation dead end product has fluorescent characteristic, under the optical excitation of 350nm-420nm, produces fluorescence at 420nm-600nm.
The present invention uses skin histology fluorescence, real-time, noninvasive human body oxidative stress status.The present invention uses the part of excitation light irradiation individual's skin tissue and detects the fluorescence that described tissue sends.The present invention on measured fluorescence spectrum, provides correlation model or diagnostic threshold that spectral information is associated with oxidative stress status the spectral manipulation algorithm application then.
The generation of fluorescence: when the excitation light irradiation skin histology, some molecule that is excited transits to excitation level from low-lying level, excitation level is unsettled, therefore molecule transits to than low-lying level from higher energy level, this process is accompanied by the emission of fluorescence, and the wavelength of fluorescence of release is always longer than excitation wavelength.
The propagation of light: when excitation light irradiation is to skin histology, can experience scattering and absorption process, this is relevant with distance between optical property, excitation wavelength, acquisition system and the tested skin at illuminated position.The fluorescence that is produced also can experience relevant absorption and scattering.Therefore, the skin difference of tested object determines that to final the tissue oxidizing stress state has significant impact.
Spectrum correction: the present invention interested histofluorescence be its primary fluorescence---the light that thin sample sends on homogeneous, no scattering and the optics.In order to obtain the intrinsic fluorescence spectra of skin histology, carry out spectrum correction at different tested objects, obtain the intrinsic fluorescence spectra of molecules of interest, thereby judge the oxidative stress status of tested object.
F in the following formula
Meas(λ
x, λ
m) be in excitation wavelength lambda
xExcite down measured skin histology fluorescence spectrum, R
Meas(λ
m) be the reflectance of skin histology measured position, F
Corr(λ
x, λ
m) be the fluorescence spectrum after the correction skin difference.
λ in the following formula
1-λ
2Be fluorescence wave band, λ
3-λ
4For exciting wave band, after the influence that the calibration light source instability is brought, A.F. can the interior terminal glycosylation dead end product molecule content of antimer.
A kind of human body oxidative stress non-invasive fluorescence detection device, see accompanying drawing 1: include near ultraviolet light source 1, narrow band pass filter 2 near ultraviolet light source 1 emitting light path and beam splitter 3, coupled lens 4, fibre-optical probe 5, spectrogrph 7, exciting light conduction optical fiber 9 and exciting light receive optical fiber 10, and spectral signal analysis process system 8.Human test position skin 6 places fibre-optical probe 5 the place aheads.
Described near ultraviolet light source 1 has the wavelength of 300nm-450nm scope, can be the combination of wideband light source or a plurality of narrow-band light source;
Described optical filter 2 is combinations of narrow band pass filter or a plurality of cut-off type optical filters;
Described beam splitter 3 is that to be used to produce wavelength identical, the two-beam that the direction of propagation is different, i.e. exciting light and reference light;
Described lens 4 are lens combinations, and purpose is that the coupling exciting light enters conduction optical fiber;
Described fibre-optical probe 5 is made up of conduction optical fiber and collection optical fiber, can be polymorphic fibre bundle;
Described spectrogrph 7 is the high accuracy spectrogrphs that can detect 350nm-600nm band spectrum signal;
Described computer 8 is that the spectral signal analysis process system is used for the spectral signal that analyzing and processing collected, and finally obtains the oxidative stress status of measurand.
Operation principle of this device and groundwork process prescription are as follows:
The light that near ultraviolet light source 1 produces, it is identical to form two bundle wavelength through optical filter 2 and beam splitter 3, the monochromatic light that the direction of propagation is different, wherein a branch of exciting light I that serves as
1(λ), another does up reference light I
2(λ) effect.Exciting light is coupled into exciting light conduction optical fiber 9 through lens 4, to fibre-optical probe 5; Human test position skin 6 is close to fibre-optical probe 5 end faces, after the monochromatic light irradiation through become miter angle with it, the terminal glycosylation dead end product is stimulated and produces the fluorescence of 420nm-600nm in the tissue, and fluorescence overflows skin histology afterwards by the collection collecting fiber of fibre-optical probe 5; Excitation source is radiated at reflected light and the scattered light that produces on the skin histology, by the reception collecting fiber of fibre-optical probe, conducts to spectrogrph 7 equally.The reference light that beam splitter 3 produces is directly conducted to spectrogrph 7 through optical fiber, and the spectrogrph optical signal is converted into the signal of telecommunication after serial ports transfers to spectrum analysis processing system 8.
In conjunction with F
Meas(λ
x, λ
m), R
Meas(λ
m) and I
2(λ), at first proofread and correct the influence of skin scattering absorption to test result, adopt peak integration fado variable spectroscopic data treatment technology (PIMS then, Peak Integration Method Spectro), the calibration light source instability is to the influence of measurement result, and the A.F. value can reflect measurand skin terminal glycosylation dead end product relative concentration.The A.F. value of different tested objects contrasts as shown in Figure 5, and the A.F. value of utilizing the oxidative stress person that this device records is apparently higher than the normal person.
Utilize above-mentioned A.F. value, simultaneously the other biological of collecting test object is learned information, as age, height, body weight, whether smoking, whether suffer from the biochemistry detection result of diabetes, vivo oxidation stress state etc., carries out comprehensive analysis processing.
At first utilize multiple regression analysis and Principal Component Analysis Method, analyze the influence that each information is passed judgment on oxidative stress status, to reduce the dimension that obtains data.Then, take multiple regression analysis, covariance analysis, neural network BP training algorithm is set up fluorescence-oxidative stress status model.Finally, according to the non-invasive measurement result, and, can judge and organize oxidative stress status in conjunction with this model.
Embodiment:
Human body oxidative stress non-invasive fluorescence detection device includes excitation light source system, spectra collection system and spectral signal analysis process system.
The example I of this kind checkout gear comprises high power xenon lamp, monochromator and the coupled lens group as the excitation light source system core parts; Comprise conduction optical fiber, fibre-optical probe and spectrogrph as the spectra collection system, wherein fibre-optical probe is shown in Fig. 2 (a), it is the combination of 1 central optical fiber and 12 edge optical fiber, conduction optical fiber connects device with fibre-optical probe by optical fiber interconnections and is connected, and spectrogrph is the high accuracy spectrogrph that can detect 350nm-600nm band spectrum signal; Comprise computer and interpretation software as the spectral signal analytical system.
Use high power xenon lamp, monochromator and coupled lens group among the near ultraviolet multi-wavelength LED replacement example I among the example II.
With replace the fibre-optical probe shown in 2 (a) among the example I as the fibre-optical probe as shown in Fig. 2 (b) or 2 (c), the structure that changes fibre-optical probe can change the skin depth that can detect among the example III.
Install described in the application example I, detect at different tested objects.Fig. 4 is the original fluorescence spectrum F that partial test subject's skin tissue is stimulated and produces
Meas(λ
x, λ
m), at the 420nm-600nm wave band, the tested object fluorescence spectrum that the oxidative stress degree is high obviously is better than the normal person.
Fig. 5 is the skin emissivity that the partial test object measures by non-invasive fluorescence detection device, and the skin reflectivity difference of different tested objects to the absorption and the scattering degree difference of light, thereby can cause measured fluorescence spectrum to distort.Proofread and correct its fluorescence spectrum according to different tested objects skin reflectivity separately, and carry out analyzing and processing, obtain reflecting the index of its fluorescence intensity at last, as shown in Figure 6.Its index of tested object that the oxidative stress degree is high generally is higher than the normal person.
Fig. 7 is that the box of different tested object oxidative stress status cross validation posterior probability must figure.As seen from the figure, exist probability that the tested object of oxidative stress detects oxidative stress, but can not find the person that to distinguish the oxidative stress fully and normal person's single threshold value apparently higher than the normal person.Common method is that test result is carried out specificity-sensitivity analysis, draws specificity-sensitivity curves.Experimenter's performance curve is as shown in Figure 7: area under a curve has reached 0.868, with accidental at random test value 0.5 relatively, difference has statistical significance, can being applied to clinically, whether the diagnostic test object exists oxidative stress.
Obviously, those skilled in the art can carry out various changes and modification to non-invasive fluorescence detection device of the present invention and method and not break away from the spirit and scope of the present invention.Like this, if of the present invention these are revised and modification belongs within the scope of claim of the present invention and equivalent technologies thereof, then the present invention also is intended to comprise these changes and modification interior.
Claims (10)
1. human body oxidative stress non-invasive fluorescence detection device includes excitation light source system, spectra collection system and spectral signal analysis process system:
Excitation light source system comprises the near ultraviolet light source, optical filter and beam splitter near ultraviolet light source emitting light path, and beam splitter is divided into reference light and exciting light to emergent light;
The spectra collection system comprises conduction optical fiber, fibre-optical probe and spectrogrph; Conduction optical fiber is used for reference light is transferred to spectrogrph; Fibre-optical probe is used to conduct exciting light to tested human body skin part, collects human body skin because of exciting the fluorescence that produces and transferring to spectrogrph; Spectrogrph is used for the detection of spectral signal;
The spectral signal analysis process system is used for the spectral signal that analyzing and processing collected, and finally obtains the oxidative stress status of measurand.
2. human body oxidative stress non-invasive fluorescence detection device according to claim 1 is characterized in that described near ultraviolet light source has the wavelength of 300nm-450nm scope, can be the combination of wideband light source or a plurality of narrow-band light source.
3. human body oxidative stress non-invasive fluorescence detection device according to claim 1 is characterized in that described optical filter is the combination of narrow band pass filter or a plurality of cut-off type optical filters.
4. human body oxidative stress non-invasive fluorescence detection device according to claim 1 is characterized in that conducting the spectral signal that optical fiber can transmit 300nm-600nm, can be the fibre bundle that optical fiber or multifiber are formed.
5. human body oxidative stress non-invasive fluorescence detection device according to claim 1 is characterized in that described fibre-optical probe by conducting optical fiber and collecting optical fiber and form, can be polymorphic fibre bundle.
6. human body oxidative stress non-invasive fluorescence detection device according to claim 1 is characterized in that the spectral signal acquisition system gathers the spectral signal through skin histology reflection and scattering simultaneously, determines calibrated fluorescence measurement value by the reflection characteristic of organizing.
7. human body oxidative stress non-invasive fluorescence detection device according to claim 1, it is characterized in that the biological information of spectral signal analysis process system with tested object, the model that calibrated fluorescence measurement value and structural state are associated is in conjunction with the state with definite described tissue.
8. human body oxidative stress non-invasive fluorescence detection device according to claim 7, wherein said biological information comprise the melanin and the haemachrome level of individual race, sex, height, body weight, whether smoking, family's medical history, skin.
9. human body oxidative stress non-invasive fluorescence detection device according to claim 7, wherein said model is used for method of least square, covariance analysis method, PCA, multiple regression analysis method, neural network algorithm the mensuration of structural state by the fluorescent characteristic of a plurality of tested object skin histologies and the skin histology state of tested object are detected.
10. human body oxidative stress non-invasive fluorescence detection method is characterized in that may further comprise the steps:
The light that the near ultraviolet light source produces, it is identical to form two bundle wavelength through optical filter and beam splitter, and the monochromatic light that the direction of propagation is different is wherein a branch of as exciting light I
1(λ), another bundle conduct is with reference to light I
2(λ); Exciting light transfers to human test position skin by conduction optical fiber, the terminal glycosylation dead end product is stimulated and produces the fluorescence of 420nm-600nm in the skin histology of human test position, fluorescence is by receiving collecting fiber, and the exciting light of skin histology reflection and scattering also is received collecting fiber simultaneously; To spectrogrph, the measured signal note of spectrogrph is made F to the spectrum that reception optical fiber is collected through fiber optic conduction
Meas(λ
x, λ
m); Reference light I
2(λ) be directly conducted to described spectrogrph through optical fiber, spectrogrph transfers to computer after optical signal is converted into the signal of telecommunication.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910185525A CN101716069A (en) | 2009-11-17 | 2009-11-17 | Human body oxidative stress non-invasive fluorescence detection device and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910185525A CN101716069A (en) | 2009-11-17 | 2009-11-17 | Human body oxidative stress non-invasive fluorescence detection device and method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101716069A true CN101716069A (en) | 2010-06-02 |
Family
ID=42430769
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910185525A Pending CN101716069A (en) | 2009-11-17 | 2009-11-17 | Human body oxidative stress non-invasive fluorescence detection device and method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101716069A (en) |
Cited By (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013067996A1 (en) * | 2011-11-10 | 2013-05-16 | Institut Für Photonische Technologien E.V. | Optical fibre for the filtered collection of light, in particular raman scattered light and method for the production thereof |
| CN103167830A (en) * | 2010-06-18 | 2013-06-19 | 代克诺普提克斯控股有限公司 | Method and apparatus for determining an autofluorescence value of skin tissue |
| CN103245650A (en) * | 2013-05-10 | 2013-08-14 | 铜陵易康达光电科技有限公司 | Noninvasive diabetes detection device based on advanced glycation end product fluorescence spectrum |
| CN103565410A (en) * | 2012-07-09 | 2014-02-12 | 韩国电气研究院 | Transmitted light detection type measurement apparatus for skin autofluorescence |
| CN104713647A (en) * | 2015-03-10 | 2015-06-17 | 张美英 | Spectrograph and spectral analysis method |
| CN105474013A (en) * | 2013-08-19 | 2016-04-06 | 西门子医学诊断产品有限责任公司 | Analysis method for supporting classification |
| CN105939656A (en) * | 2014-02-04 | 2016-09-14 | 皇家飞利浦有限公司 | Optical device for measuring a heart rate of a user |
| WO2016180095A1 (en) * | 2015-05-08 | 2016-11-17 | 上海交通大学 | Method for detecting, in noninvasive in-vivo manner, skin damage induced by ultraviolet light, and detection device thereof |
| CN106353295A (en) * | 2016-11-11 | 2017-01-25 | 中国科学院生物物理研究所 | Protein heat stability analysis meter |
| CN106606356A (en) * | 2015-10-23 | 2017-05-03 | 上海新微技术研发中心有限公司 | Physical sign parameter measurement wearable device based on sensing technology |
| CN106606357A (en) * | 2015-10-23 | 2017-05-03 | 上海新微技术研发中心有限公司 | Physical sign parameter measuring mask based on sensing technology |
| CN106706585A (en) * | 2016-12-31 | 2017-05-24 | 中国科学院昆明动物研究所 | Device and method for recording living body fluorescence signals |
| CN106872421A (en) * | 2016-12-29 | 2017-06-20 | 上海交通大学 | A kind of method of markless detection cell or tissue oxidative stress and its application |
| US9723990B2 (en) | 2012-03-21 | 2017-08-08 | Korea Electro Technology Research Institute | Transmitted light detection type measurement apparatus for skin autofluorescence |
| US9955871B2 (en) | 2012-03-21 | 2018-05-01 | Korea Electrotechnology Research Institute | Transmitted light detection type measurement apparatus for skin autofluorescence |
| CN108007910A (en) * | 2017-12-28 | 2018-05-08 | 中国科学院合肥物质科学研究院 | It is a kind of to be used for skin histology fluorescence spectrum and the depth sensitive optical fibre probe of rear orientation light spectrum in site measurement |
| CN108604376A (en) * | 2016-02-08 | 2018-09-28 | 皇家飞利浦有限公司 | Equipment, system and method for pulsation detection |
| CN109316169A (en) * | 2018-11-03 | 2019-02-12 | 复旦大学 | A kind of health detector and its detection method based on mobile intelligent terminal |
| CN109793501A (en) * | 2019-03-08 | 2019-05-24 | 合肥中科易康达生物医学有限公司 | A kind of body metabolism health index Non-invasive detection equipment |
| DE102017222530A1 (en) * | 2017-12-12 | 2019-06-13 | Henkel Ag & Co. Kgaa | Arrangement for determining metabolic end products in the skin |
| CN110313892A (en) * | 2018-03-28 | 2019-10-11 | 上海交通大学 | The pathogenetic method and its application of Parkinson are judged based on body surface autofluorescence |
| CN111665198A (en) * | 2020-07-24 | 2020-09-15 | 惠州清水湾生物材料有限公司 | Portable fluorescence fiber spectrometer |
| CN113720825A (en) * | 2021-11-04 | 2021-11-30 | 四川丹诺迪科技有限公司 | Optical instant detector and detection method and application |
-
2009
- 2009-11-17 CN CN200910185525A patent/CN101716069A/en active Pending
Cited By (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103167830B (en) * | 2010-06-18 | 2016-08-31 | 代克诺普提克斯控股有限公司 | For the method and apparatus determining the autofluorescence value of skin histology |
| CN103167830A (en) * | 2010-06-18 | 2013-06-19 | 代克诺普提克斯控股有限公司 | Method and apparatus for determining an autofluorescence value of skin tissue |
| WO2013067996A1 (en) * | 2011-11-10 | 2013-05-16 | Institut Für Photonische Technologien E.V. | Optical fibre for the filtered collection of light, in particular raman scattered light and method for the production thereof |
| US9723990B2 (en) | 2012-03-21 | 2017-08-08 | Korea Electro Technology Research Institute | Transmitted light detection type measurement apparatus for skin autofluorescence |
| US9955871B2 (en) | 2012-03-21 | 2018-05-01 | Korea Electrotechnology Research Institute | Transmitted light detection type measurement apparatus for skin autofluorescence |
| CN103565410B (en) * | 2012-07-09 | 2016-05-18 | 韩国电气研究院 | For the transmitted light detection type measurement device of skin autofluorescence |
| CN103565410A (en) * | 2012-07-09 | 2014-02-12 | 韩国电气研究院 | Transmitted light detection type measurement apparatus for skin autofluorescence |
| CN103245650A (en) * | 2013-05-10 | 2013-08-14 | 铜陵易康达光电科技有限公司 | Noninvasive diabetes detection device based on advanced glycation end product fluorescence spectrum |
| CN103245650B (en) * | 2013-05-10 | 2015-01-28 | 铜陵易康达光电科技有限公司 | Noninvasive diabetes detection device based on advanced glycation end product fluorescence spectrum |
| US10401275B2 (en) | 2013-08-19 | 2019-09-03 | Siemens Healthcare Diagnostics Products Gmbh | Analysis method for supporting classification |
| CN105474013B (en) * | 2013-08-19 | 2018-09-18 | 西门子医学诊断产品有限责任公司 | For the analysis method supported of classifying |
| CN105474013A (en) * | 2013-08-19 | 2016-04-06 | 西门子医学诊断产品有限责任公司 | Analysis method for supporting classification |
| CN105939656A (en) * | 2014-02-04 | 2016-09-14 | 皇家飞利浦有限公司 | Optical device for measuring a heart rate of a user |
| CN105939656B (en) * | 2014-02-04 | 2019-06-28 | 皇家飞利浦有限公司 | For measuring the optical device of user's heart rate |
| CN104713647A (en) * | 2015-03-10 | 2015-06-17 | 张美英 | Spectrograph and spectral analysis method |
| WO2016180095A1 (en) * | 2015-05-08 | 2016-11-17 | 上海交通大学 | Method for detecting, in noninvasive in-vivo manner, skin damage induced by ultraviolet light, and detection device thereof |
| CN111134618A (en) * | 2015-05-08 | 2020-05-12 | 江苏坤辉生物科技有限公司 | Method and device for in vivo non-invasive detection of ultraviolet light induced skin injury |
| CN106606356A (en) * | 2015-10-23 | 2017-05-03 | 上海新微技术研发中心有限公司 | Physical sign parameter measurement wearable device based on sensing technology |
| CN106606357A (en) * | 2015-10-23 | 2017-05-03 | 上海新微技术研发中心有限公司 | Physical sign parameter measuring mask based on sensing technology |
| CN108604376A (en) * | 2016-02-08 | 2018-09-28 | 皇家飞利浦有限公司 | Equipment, system and method for pulsation detection |
| CN108604376B (en) * | 2016-02-08 | 2022-04-01 | 皇家飞利浦有限公司 | Device, system and method for pulsatility detection |
| CN106353295A (en) * | 2016-11-11 | 2017-01-25 | 中国科学院生物物理研究所 | Protein heat stability analysis meter |
| CN106872421A (en) * | 2016-12-29 | 2017-06-20 | 上海交通大学 | A kind of method of markless detection cell or tissue oxidative stress and its application |
| CN106706585A (en) * | 2016-12-31 | 2017-05-24 | 中国科学院昆明动物研究所 | Device and method for recording living body fluorescence signals |
| DE102017222530A1 (en) * | 2017-12-12 | 2019-06-13 | Henkel Ag & Co. Kgaa | Arrangement for determining metabolic end products in the skin |
| CN108007910A (en) * | 2017-12-28 | 2018-05-08 | 中国科学院合肥物质科学研究院 | It is a kind of to be used for skin histology fluorescence spectrum and the depth sensitive optical fibre probe of rear orientation light spectrum in site measurement |
| CN108007910B (en) * | 2017-12-28 | 2023-12-29 | 中国科学院合肥物质科学研究院 | Depth-sensitive optical fiber probe for in-situ measurement of fluorescence spectrum and back-scattered light spectrum of skin tissue |
| CN110313892A (en) * | 2018-03-28 | 2019-10-11 | 上海交通大学 | The pathogenetic method and its application of Parkinson are judged based on body surface autofluorescence |
| CN109316169A (en) * | 2018-11-03 | 2019-02-12 | 复旦大学 | A kind of health detector and its detection method based on mobile intelligent terminal |
| CN109793501A (en) * | 2019-03-08 | 2019-05-24 | 合肥中科易康达生物医学有限公司 | A kind of body metabolism health index Non-invasive detection equipment |
| CN111665198A (en) * | 2020-07-24 | 2020-09-15 | 惠州清水湾生物材料有限公司 | Portable fluorescence fiber spectrometer |
| CN113720825A (en) * | 2021-11-04 | 2021-11-30 | 四川丹诺迪科技有限公司 | Optical instant detector and detection method and application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101716069A (en) | Human body oxidative stress non-invasive fluorescence detection device and method | |
| JP5053639B2 (en) | Determination of glycation end products or disease state measures using tissue fluorescence | |
| US7343185B2 (en) | Measurement of body compounds | |
| US7725144B2 (en) | Determination of disease state using raman spectroscopy of tissue | |
| CN100998499B (en) | Determination of a measure of a glycation end-product or disease state using tissue fluorescence | |
| KR20120130164A (en) | Method and apparatus to detect coronary artery calcification or disease | |
| CA2383727A1 (en) | Method for determination of analytes using near infrared, adjacent visible spectrum and an array of longer near infrared wavelengths | |
| CA2597859A1 (en) | Method and apparatus for determining blood analytes | |
| JP4361822B2 (en) | Method and apparatus for measuring component concentration of target object | |
| US20190274601A1 (en) | Non-invasive blood analysis | |
| US20080269616A1 (en) | Mir spectroscopy of tissue | |
| CN210300954U (en) | Noninvasive human body metabolic health index detection equipment | |
| EP4137063A1 (en) | Hemoglobin concentration measuring system, transvaginal probe, attachment, and hemoglobin concentration measuring method | |
| CN103245650B (en) | Noninvasive diabetes detection device based on advanced glycation end product fluorescence spectrum | |
| KR20090036996A (en) | Non-prick based glucose sensor combining transmittance and reflectance using single wavelength with diverse light sources | |
| CN203303031U (en) | Diabetes non-invasive detecting device based on glycation end product fluorescence spectrum | |
| JPH09113439A (en) | Method and apparatus for measuring organic component | |
| US20230148312A1 (en) | Device for non-invasive blood glucose concentration measurement | |
| JP2009247430A (en) | Non-invasive discriminating method of skin age | |
| JP2011220993A (en) | Near-infrared spectroscopic analysis apparatus | |
| WO2012127378A1 (en) | An apparatus for optical analysis of an associated tissue sample | |
| KR20070017479A (en) | Determination of a Measure of a Glycation End-Product or Disease State Using Tissue Fluorescence |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20100602 |