CN111722262A - Scintillation absorption liquid - Google Patents
Scintillation absorption liquid Download PDFInfo
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- CN111722262A CN111722262A CN202010600696.7A CN202010600696A CN111722262A CN 111722262 A CN111722262 A CN 111722262A CN 202010600696 A CN202010600696 A CN 202010600696A CN 111722262 A CN111722262 A CN 111722262A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01T—MEASUREMENT OF NUCLEAR OR X-RADIATION
- G01T1/00—Measuring X-radiation, gamma radiation, corpuscular radiation, or cosmic radiation
- G01T1/16—Measuring radiation intensity
- G01T1/20—Measuring radiation intensity with scintillation detectors
- G01T1/204—Measuring radiation intensity with scintillation detectors the detector being a liquid
- G01T1/2042—Composition for liquid scintillation systems
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
- G01N21/80—Indicating pH value
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01T—MEASUREMENT OF NUCLEAR OR X-RADIATION
- G01T1/00—Measuring X-radiation, gamma radiation, corpuscular radiation, or cosmic radiation
- G01T1/16—Measuring radiation intensity
- G01T1/20—Measuring radiation intensity with scintillation detectors
- G01T1/204—Measuring radiation intensity with scintillation detectors the detector being a liquid
- G01T1/2042—Composition for liquid scintillation systems
- G01T1/2047—Sample preparation
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Abstract
The invention discloses a scintillation absorption liquid, which comprises the following components: the composite material comprises a nonionic surfactant, linear alkylbenzene, an alcohol solvent, an organic scintillator, a second scintillator, a carbon dioxide absorbent and an acid-base indicator; wherein, the alcohol solvent is methanol or ethanol or a mixture of the methanol and the ethanol, the carbon dioxide absorbent is alkali metal hydroxide, the first scintillator and/or the second scintillator are/is added into linear alkylbenzene with required amount, and the linear alkylbenzene is heated and dissolved; adding the carbon dioxide absorbent and/or the indicator into the required amount of alcoholic solution, stirring and dissolving, and filtering off insoluble substances; mixing the above two solutions with required amount of nonionic surfactant to obtain scintillation absorption solution for diagnosing helicobacter pylori14C urea breath test, collecting CO in breath2And counting and measuring in a scintillation counter, the scintillation absorption liquid of the invention can overcome the defects of the existing liquid scintillation method, card method, solid scintillation method and the like, and has the advantages of easy storage, high accuracy and useConvenient and fast.
Description
Technical Field
The invention belongs to the field of medical diagnosis, and particularly relates to a method for diagnosing helicobacter pylori14The urea breath test collects breath samples and measured scintillation absorption solution.
Background
14The C-urea breath test is the main method for detecting helicobacter pylori infection, and the principle is that the test person takes orally the characteristic that helicobacter pylori generates high-activity urease14The C-labelled urea is then decomposed to give14CO2Is discharged through expiration and is measured for the markers in the expired gas14CO2The presence or absence of helicobacter pylori infection can be judged by changing conditions. The prior art adopts methods such as liquid scintillation, card method, solid scintillation and the like to carry out the process14C-label breath test samples were collected and measured.
Among them, the liquid scintillation method is a method of dissolving an organic amine compound in a methanol solution to form CO2Absorbing liquid is placed in a scintillation bottle, gas exhaled by an examinee is introduced into the absorbing solution through a thin tube inserted into the solution to collect a carbon dioxide sample, the absorbing solution absorbing the carbon dioxide sample is added with the scintillation liquid and mixed evenly, and then the radioactivity of the sample is measured through a liquid scintillation counter. The method comprises the steps of collecting carbon dioxide in exhaled air by using absorption liquid, adding scintillation liquid, and measuring, wherein the use is troublesome; and toxic solvents such as toluene, xylene and trimethylbenzene are used, organic amines such as hydrabamine hydroxide, phenethylamine and ethanolamine are used as absorbents, and the defects of poor stability and low-temperature storage are also existed. Although the card method and the solid scintillation method are convenient to use, do not contain toxic solvents and can be stored at normal temperature, the card method and the solid scintillation method have the defects of insufficient sample amount and low detection efficiency.
Disclosure of Invention
The invention aims to provide a scintillation absorption liquid which can overcome the defects of a liquid scintillation method, a cassette method, a solid scintillation method and the like and is used for diagnosing helicobacter pylori infection14C urea breath test, collecting CO in breath2And counting and measuring in a scintillation counter, and has the advantages of easy storage, high accuracy and convenient and fast use.
The purpose of the invention can be realized by the following technical scheme:
a scintillation absorption liquid comprises the following components: the composite material comprises a nonionic surfactant, linear alkylbenzene, an alcohol solvent, an organic scintillator, a second scintillator, a carbon dioxide absorbent and an acid-base indicator;
the components and the proportion of the components are as follows:
nonionic surfactant: such as alkylphenol ethoxylates, alkylphenyl polyoxyethylene ether, fatty alcohol polyoxyethylene ether and the like, preferably Triton X-100(Triton X-100), and is nontoxic, tasteless and nonflammable; the addition amount of the nonionic surfactant is 10-60% by volume, preferably 20-50%;
the linear alkylbenzene is C6-C15 linear alkylbenzene, preferably dodecyl benzene and industrial linear alkylbenzene, is usually used as a detergent for production, is nontoxic, tasteless, high in boiling point and nonflammable; the addition amount of the linear alkyl benzene is 10-60% by volume, preferably 20-50%;
the alcohol solvent is methanol, ethanol, isopropanol, ethylene glycol, propylene glycol, etc., or mixture of any components thereof, preferably methanol or ethanol or mixture thereof; the addition amount of the alcohol solvent is 5-50% by volume, preferably 20-40%;
organic scintillators, also referred to as first scintillators, such as: PPO, PBO, PBD and the like, preferably PPO, and the addition amount of the organic scintillator is 1-8g/L, preferably 3-6 g/L;
the second scintillator can be selectively added (can be added), and the second scintillator can change the wavelength of the scintillation light, so that the detection efficiency is improved, for example: POPOP, DM-POPOPOPOP, bisMSB, etc., preferably POPOPOP; the addition amount of the second scintillator is 0-0.5g/L, preferably 0.04-0.4 g/L;
in the prior art, organic amines such as hydrabamine hydroxide, phenethylamine, ethanolamine and the like are used as carbon dioxide absorbents, and a scintillation system is homogeneous liquid or emulsion, so that the price is high, the stability is poor, and the carbon dioxide absorbent needs to be stored at low temperature; the method has the advantages that the alkali metal hydroxide is used as the carbon dioxide absorbent, the price is low, the stability is good, the carbon dioxide absorbent can be stored at normal temperature, the sodium hydroxide is used as the absorbent, the performance is better, the ratio of all components is adjusted, the scintillation absorption liquid is homogeneous, the solution can be converted into a colloid state after the carbon dioxide is absorbed, the sampling end point can be indicated under the condition that an acid-base indicator is not added, and the splashing is not easy; the adding amount of the carbon dioxide absorbent is 0.05-0.5mol/L, preferably 0.08-0.35 mol/L;
optionally, an acid-base indicator can be added (optionally), wherein the acid-base indicator comprises phenolphthalein, bromothymolphthalein and the like to indicate a sampling end point; the acid-base indicator is added according to the conventional dosage, so that the solution presents an obviously observable color.
The preparation method of the scintillation absorption liquid comprises the following steps:
s1, adding the organic scintillator and/or the second scintillator into linear alkylbenzene with required amount, and heating for dissolving;
s2, adding the carbon dioxide absorbent and/or the acid-base indicator into a required amount of alcohol solvent, stirring for dissolving, and filtering out insoluble substances;
and S3, mixing the two solutions with the required amount of the nonionic surfactant to obtain the scintillation absorption liquid.
The invention has the beneficial effects that:
compared with the existing liquid scintillation method, the scintillation absorption liquid of the invention has the following advantages:
A. with the same background count rate and detection efficiency.
B. The method is simple and convenient: in the prior art, carbon dioxide in exhaled air is collected by using absorption liquid, scintillation liquid is added, and then measurement is carried out.
C. And (3) harm reduction: volatile toxic solvents such as toluene, xylene and trimethylbenzene are not used.
D. The reliability is high: the end point judgment is obvious, after the sampling is finished, the scintillation absorption liquid can be changed into a colloid state from a liquid state, splashing is not easy, and after the step of adding the scintillation liquid is omitted, cross contamination is avoided.
E. In the prior art, organic amine is used as an absorbent, so that the stability is poor, low-temperature storage is required, and the organic amine can be stored at normal temperature.
Compared with the card method, the scintillation absorption liquid of the invention:
1) the method has the same convenience as the card method.
2) Compared with a card method, the method has the advantages of high detection efficiency, high signal-to-noise ratio and higher accuracy.
3) Low production cost and high production efficiency.
Compared with a solid flash method, the scintillation absorption liquid of the invention comprises the following steps:
1. has the same convenience as that of the solid flash method.
2. Compared with a solid-flash method, the method has the advantages of high detection efficiency, high signal-to-noise ratio and higher accuracy.
3. Low production cost and high production efficiency.
Drawings
The invention will be further described with reference to the accompanying drawings.
FIG. 1 is a drawing of example 1 of the present invention14C, scintillation counting spectrogram;
FIG. 2 is a sample of the prior art14C scintillation counting spectrogram.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The formula of the scintillation absorption liquid is as follows: triton X-100: 100 mL; industrial linear alkylbenzenes: 100 mL; anhydrous ethanol: 89 mL; PPO: 1.45 g; POPOP: 0.08 g; sodium hydroxide: 0.333 mmol; phenolphthalein indicator: 0.07 g;
the preparation method comprises the following steps:
s1, adding PPO and POPOPOP into linear alkylbenzene with required amount, and heating to dissolve;
s2, adding sodium hydroxide and an indicator into the absolute ethyl alcohol solution with required amount, stirring and dissolving, and filtering out insoluble substances;
s3, mixing the two solutions with the required amount of triton X-100 to obtain scintillation absorption liquid;
the prepared scintillation absorption solution is subpackaged with 6.5mL of scintillation vial in 20mL scintillation vial.
Comparative analysis with prior art count spectra:
respectively collecting expired air of the same positive subject by using 2mL of absorption solution containing ethanolamine and methanol in the prior art and the scintillation absorption solution of the invention, wherein the expired air contains14CO2Adding 4.5mL of scintillation fluid containing toluene and methanol into a sample in the prior art, shaking uniformly, measuring, directly measuring the scintillation absorption liquid sample, measuring the sample for 10 minutes by using a Hidex300SL type liquid scintillation counter, collecting the count of a count window of 0-500 times, drawing14C scintillation counting spectrum, as shown in fig. 1 and 2;
FIG. 1 is a sample of example 114C scintillation counting spectrum, FIG. 2 of a sample of the prior art14C scintillation counting spectrum, as can be seen from FIGS. 1 and 2, the scintillation counting of the sample of the present invention and the sample of the prior art both fall within the 25-450 counting window, and the spectrum shapes substantially conform to each other, but it is clear that the counting spectrum of the present invention is slightly shifted backwards, and the counting is more in the high energy window, which indicates that the scintillation system of the present invention has a lower quenching degree for β ray energy.
Compared with the use effect of the prior art, the test method comprises the following steps:
administered to 25 subjects14After 1 urea capsule (27.75KBq), waiting for 25 minutes, collecting a first sample by using 2mL of absorption liquid according to the existing method, then collecting a second sample, namely the scintillation absorption liquid sample, by using a scintillation bottle filled with the scintillation absorption liquid, and adding 4.5mL of scintillation liquid into the first sample and uniformly mixing to obtain a sample in the prior art;
otherwise, it is not taken14The operator of the urea capsule C collects two blank samples as background in two ways.
All samples were measured on a Hidex300SL model liquid scintillation counter for 3 minutes and the net count rate results measured are expressed in counts per minute (cpm), usually a net count rate of > 50cpm is judged as H.pylori infection (positive +).
Test results comparative data are as follows:
as can be seen from the above table, the measured counts of all samples of the present invention compared to the prior art samples were substantially equivalent, judgedThe results are completely consistent, although the human breath is excluded14CO2The amount varies with time and varies greatly among individuals, and the sample is collected14CO2The amount of the interference factors is not controllable, but the test result shows that the invention still has good consistency compared with the prior art.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing is merely exemplary and illustrative of the present invention and various modifications, additions and substitutions may be made by those skilled in the art to the specific embodiments described without departing from the scope of the invention as defined in the accompanying claims.
Claims (7)
1. A scintillation absorption liquid is characterized by comprising the following components: the composite material comprises a nonionic surfactant, linear alkylbenzene, an alcohol solvent, an organic scintillator, a second scintillator, a carbon dioxide absorbent and an acid-base indicator;
the components and the proportion of the components are as follows:
the nonionic surfactant is alkylphenol polyoxyethylene, alkylphenyl polyoxyethylene or fatty alcohol polyoxyethylene; the addition of the nonionic surfactant is 10-60% by volume ratio;
the linear alkylbenzene is linear alkylbenzene between C6 and C15; the adding amount of the linear alkylbenzene is 10-60% according to the volume ratio;
the alcohol solvent is methanol or ethanol or isopropanol or ethylene glycol or propylene glycol or a mixture of any components thereof; the adding amount of the alcohol solvent is 5-50% according to the volume ratio;
the organic scintillator is called a first scintillator and is PPO, PBO or PBD; the adding amount of the organic scintillator is 1-8 g/L;
the second scintillator is POPOPOP, DM-POPOPOP or bis MSB; the adding amount of the second scintillator is 0-0.5 g/L;
the carbon dioxide absorbent is sodium hydroxide or lithium hydroxide or potassium hydroxide or a mixture of any components of the sodium hydroxide or the lithium hydroxide or the potassium hydroxide; the adding amount of the carbon dioxide absorbent is 0.05-0.5 mol/L;
the acid-base indicator comprises phenolphthalein and bromothymolphthalein; adding an acid-base indicator according to the conventional dosage;
the preparation method of the scintillation absorption liquid comprises the following steps:
s1, adding the organic scintillator and/or the second scintillator into linear alkylbenzene with required amount, and heating for dissolving;
s2, adding the carbon dioxide absorbent and/or the acid-base indicator into a required amount of alcohol solvent, stirring for dissolving, and filtering out insoluble substances;
and S3, mixing the two solutions with the required amount of the nonionic surfactant to obtain the scintillation absorption liquid.
2. The scintillation absorption liquid according to claim 1, wherein the nonionic surfactant is triton X-100, and the addition amount of the nonionic surfactant is 20 to 50% by volume.
3. The scintillation absorption liquid according to claim 1, wherein the linear alkyl benzene is dodecyl benzene, industrial linear alkyl benzene, and the linear alkyl benzene is added in a volume ratio of 20-50%.
4. The scintillation absorption liquid according to claim 1, wherein the alcohol solvent is methanol or ethanol or a mixture of the methanol and the ethanol in any proportion, and the addition amount of the alcohol solvent is 20-40% by volume.
5. The scintillation absorption liquid according to claim 1, wherein the organic scintillator is PPO, and the amount of the added organic scintillator is 3-6 g/L.
6. The scintillation absorption liquid according to claim 1, wherein the second scintillator is popp, and the amount of the second scintillator added is 0.04 to 0.4 g/L.
7. The scintillation absorption liquid of claim 1, wherein the carbon dioxide absorbent is sodium hydroxide, and the addition amount of the sodium hydroxide is 0.08-0.35 mol/L.
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| CN202010600696.7A CN111722262A (en) | 2020-06-28 | 2020-06-28 | Scintillation absorption liquid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115128659A (en) * | 2022-05-27 | 2022-09-30 | 中国医学科学院放射医学研究所 | [ 14 C]High-efficiency absorption scintillation liquid |
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| CN103142232A (en) * | 2013-01-11 | 2013-06-12 | 安徽养和医疗器械设备有限公司 | Expiration sampling device and application method thereof |
| CN103926240A (en) * | 2013-09-30 | 2014-07-16 | 安徽养和医疗器械设备有限公司 | Carbon dioxide absorption indicator |
| CN104931999A (en) * | 2015-03-27 | 2015-09-23 | 安徽养和医疗器械设备有限公司 | Solid scintillation vial |
| CN107229065A (en) * | 2017-05-10 | 2017-10-03 | 江苏万略医药科技有限公司 | Scintillation solution |
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2020
- 2020-06-28 CN CN202010600696.7A patent/CN111722262A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US6265221B1 (en) * | 1996-12-09 | 2001-07-24 | Noster System Ab | Device for trapping and assaying C14 labelled carbon dioxide and method of use |
| CN101149350A (en) * | 2006-09-20 | 2008-03-26 | 深圳市中核海得威生物科技有限公司 | Nontoxic environment-friendly scintillation solution |
| US20100311179A1 (en) * | 2009-06-03 | 2010-12-09 | Sarah Coulter | Method of Using 14C Measurements to Determine the Percent Natural of Cleaning Compositions |
| CN103142232A (en) * | 2013-01-11 | 2013-06-12 | 安徽养和医疗器械设备有限公司 | Expiration sampling device and application method thereof |
| CN103926240A (en) * | 2013-09-30 | 2014-07-16 | 安徽养和医疗器械设备有限公司 | Carbon dioxide absorption indicator |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115128659A (en) * | 2022-05-27 | 2022-09-30 | 中国医学科学院放射医学研究所 | [ 14 C]High-efficiency absorption scintillation liquid |
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