CN111714590A

CN111714590A - Extract for treating gastric cancer and preparation method thereof

Info

Publication number: CN111714590A
Application number: CN201910205118.0A
Authority: CN
Inventors: 石小和
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-03-18
Filing date: 2019-03-18
Publication date: 2020-09-29

Abstract

The invention belongs to the technical field of medicines, and particularly relates to a preparation method of an extract for treating gastric cancer, which comprises the following steps: obtaining vinegar and traditional Chinese medicine components, wherein the traditional Chinese medicine components comprise: periplaneta americana, dandelion, pseudo-ginseng and ginseng; after the traditional Chinese medicine components are pretreated, crushing the traditional Chinese medicine components, and sieving the crushed traditional Chinese medicine components by a sieve with 10-80 meshes to obtain powdery traditional Chinese medicine components; and extracting the powdery traditional Chinese medicine components by using vinegar to obtain an extracting solution. The vinegar is used for extracting active substances in the traditional Chinese medicine components, so that the effective active substances in the traditional Chinese medicine can be efficiently extracted and dissolved out, the content of the active substances in the medicine is improved, the preparation method is simple in process, the dosage of the raw material formula is small, the raw material formula is easy to obtain, the cost is low, and the vinegar is suitable for industrial production.

Description

Extract for treating gastric cancer and preparation method thereof

Technical Field

The invention belongs to the technical field of medicines, and particularly relates to an extract for treating gastric cancer and a preparation method thereof.

Background

Gastric cancer is a malignant tumor originated from gastric mucosa epithelium, and is clinically mainly manifested by epigastric distention and discomfort, dull pain, intolerance of food, progressive anemia and emaciation, mass in late epigastric period and persistent positive fecal occult blood. Pain and weight loss are the most common clinical symptoms of advanced gastric cancer. Patients often have more definite upper gastrointestinal symptoms, such as upper abdominal discomfort, fullness after eating, aggravation of upper abdominal pain along with the progress of the disease, appetite reduction and hypodynamia. It is also characterized by different tumor sites. Carcinoma of the gastric fundus of the cardia may have poststernal pain and progressive dysphagia; gastric cancer near the pylorus shows pyloric obstruction. The incidence of the gastric cancer is the first in various malignant tumors in China, and the gastric cancer has higher incidence and mortality. At present, stomach cancer tends to be younger due to changes in dietary structure, increased working pressure, infection with helicobacter pylori, and the like. Gastric cancer can occur in any part of the stomach, more than half of which occur in antrum, greater curvature, lesser curvature, anterior and posterior walls, etc. The treatment methods of gastric cancer include surgery, radiotherapy, chemotherapy, biotherapy and the like, and even if the methods are numerous, the treatment results are still unsatisfactory in general. The operation treatment has high risk, and simultaneously causes physical trauma to patients, and the patients need further treatment by combining chemotherapy or radiotherapy in the later period generally, and the chemotherapy or radiotherapy has great damage to normal cells of human bodies, great toxic and side effects and great physical damage. Biological antibody drugs are expensive to treat, and the treatment effect varies from person to person.

At present, drug therapy is still an important way for treating gastric cancer, but most of the existing drugs for treating gastric cancer are chemical drugs, are easy to generate drug resistance, and have large side effects and unobvious effects. The traditional Chinese medicine is the most important substitution, supplement and auxiliary treatment means for malignant tumors, and has a special effect in the aspects of treating common diseases, frequently encountered diseases, difficult and complicated diseases and the like. The traditional Chinese medicine has attracted more and more attention of people because of the advantages of unique curative effect, small toxic and side effect and the like. At present, many Chinese patent medicines for treating gastric cancer have certain effect on prostate diseases, but the existing Chinese patent medicines still have insufficient and remarkable treatment effect, and cannot effectively relieve adverse reaction of patients, improve the life quality of the patients and prolong the life cycle of the patients. And the raw material cost is difficult to obtain, the preparation process is complex and the like.

Disclosure of Invention

The embodiment of the invention aims to provide a preparation method of an extract for treating gastric cancer, and aims to solve the problems that the existing medicine for treating gastric cancer has an insufficient and obvious treatment effect, cannot effectively relieve adverse reaction of a patient, improve the life quality of the patient and prolong the life cycle of the patient.

Another object of the present invention is to provide an extract for treating gastric cancer.

In order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows:

a method for preparing an extract for treating gastric cancer comprises the following steps:

obtaining vinegar and traditional Chinese medicine components, wherein the traditional Chinese medicine components comprise: periplaneta americana, dandelion, pseudo-ginseng and ginseng;

after the traditional Chinese medicine components are pretreated, crushing the traditional Chinese medicine components, and sieving the crushed traditional Chinese medicine components by a sieve with 10-80 meshes to obtain powdery traditional Chinese medicine components;

extracting the powdery traditional Chinese medicine components by using vinegar to obtain an extracting solution;

and sequentially concentrating, filtering and sterilizing the extracting solution to obtain the extract for treating the gastric cancer.

Preferably, the vinegar is selected from: one or more of Zhangjiajiuquxiangzhi vinegar, Shanxi mature vinegar, Zhenjiang aromatic vinegar, Fujia Yongchun mature vinegar, Lanzhonggning vinegar, Henan mature turtle special vinegar, Tianjin uniflow mature vinegar, Liyunggang dripping vinegar and Beijing Longmen rice vinegar.

Preferably, the total acid concentration of the vinegar is 8-16g/100 ml.

Preferably, the preparation of the vinegar at least comprises: soaking the raw materials in water, steaming, and stewing to obtain raw materials to be fermented; and then, sequentially carrying out alcoholic fermentation, acetic fermentation, fumigating, vinegar pouring, vinegar freezing and aging on the raw materials to be fermented.

Preferably, the step of pre-treating comprises: cleaning the traditional Chinese medicine components, and drying for 68-80 hours at 45-65 ℃; and/or the presence of a gas in the gas,

the method of sterilization treatment comprises: sterilizing for 30-60 minutes at the temperature of 100-110 ℃.

Preferably, the step of extracting comprises: extracting for 1-5 hours at the temperature of 20-80 ℃ and the rotating speed of 50-80 r/min, and filtering to obtain filtrate, namely extracting solution; the concentration treatment step comprises: freezing the extracting solution into solid blocks at the temperature of-25 to-18 ℃; and (3) placing the extract block at room temperature, slowly melting the extract block, collecting the molten liquid, and stopping collecting when the solid block becomes white.

Preferably, the preparation method of the extract for treating gastric cancer further comprises the steps of: adding auxiliary materials allowed in the medical scope into the extracting solution after the concentration and sterilization treatment, and preparing into pharmaceutically acceptable dosage forms.

Preferably, the adjuvant is selected from: at least one of glycerin, polysorbate, polyethylene glycol, high acyl gellan gum, xanthan gum, carrageenan, sodium alginate, sodium carboxymethylcellulose, microcrystalline cellulose, hydroxypropyl methylcellulose, crospovidone, polyacrylic resin polymers, safflower seed oil, lecithin and beeswax; and/or the presence of a gas in the gas,

the pharmaceutically acceptable dosage form comprising: at least one of oral liquid, capsule, tablet, unguent, powder, granule, pill, suppository and extract injection.

The extract for treating the gastric cancer comprises the following raw materials in parts by weight: 800 parts of vinegar, 8-20 parts of periplaneta americana, 10-20 parts of dandelion, 4-16 parts of pseudo-ginseng and 5-20 parts of ginseng as raw materials,

preferably, in the extract for treating gastric cancer, the concentration of polypeptide is 18-22 mg/ml, the concentration of polysaccharide is 12-14 mg/ml, the concentration of flavone is 22-28 mg/ml, and the concentration of ginsenoside is 3-6 mg/ml.

The preparation method of the extract for treating the gastric cancer provided by the invention comprises the steps of taking vinegar and traditional Chinese medicinal materials such as periplaneta americana, dandelion, pseudo-ginseng, ginseng and the like as main raw materials, crushing the traditional Chinese medicinal materials, taking the vinegar as an extracting agent, extracting the traditional Chinese medicinal materials, concentrating an extracting solution, and sterilizing the extracting solution to obtain the extract for treating the gastric cancer. The vinegar is used for extracting active substances in the traditional Chinese medicinal materials, so that the effective active substances in the traditional Chinese medicinal materials can be efficiently extracted and dissolved out, the content of the active substances in the medicine is improved, the preparation method is simple in process, the dosage of the raw material formula is small, the raw material formula is easy to obtain, the cost is low, the curative effect is obvious, and the vinegar is suitable for industrial production.

The extract for treating gastric cancer provided by the invention adopts vinegar as a medicinal raw material, and is matched with traditional Chinese medicine materials such as periplaneta americana, dandelion, pseudo-ginseng, ginseng and the like, so that on one hand, the components such as flavone, active peptide, polysaccharide, polyphenol and the like in the vinegar can exert the effects of various active substances; on the other hand, the vinegar can improve the dissolution of active substances in the ingredients of traditional Chinese medicinal materials such as periplaneta americana, dandelion, pseudo-ginseng, ginseng and the like, improve the content of the active substances in the medicine, realize multi-mechanism multi-target-point exertion of the medicine property through the synergistic cooperation effect of the vinegar and the active substances in the traditional Chinese medicinal materials, achieve the aims of improving the microenvironment of tumor growth, regulating tumor immune metabolism and inhibiting the proliferation and the metastasis of tumor cells, and further regulate and control the occurrence and the development of tumors. The medicine for treating gastric cancer provided by the invention has obvious analgesic, sedative and anticancer effects, reduces the toxic and side effects of the medicine while improving the medicine effect of the medicine, improves the safety of the medicine, and has excellent performance in the aspects of enhancing the chemotherapy effect, improving the immunity of the organism, inhibiting the growth of cancer cells, improving symptoms and the like, thereby achieving the purposes of improving the life quality of patients and prolonging the life cycle. And the cost is low, the economic pressure of a patient can be effectively relieved, the patient can easily accept the medicine, and the medicine is a safe and effective treatment medicine. In some embodiments, the extract for treating gastric cancer is prepared by the preparation method of the extract for treating gastric cancer.

Drawings

Fig. 1 is a schematic flow chart of a preparation method of an extract for treating gastric cancer according to an embodiment of the present invention.

Detailed Description

In order to make the purpose, technical solution and technical effect of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention is clearly and completely described, and it is obvious that the described embodiments are a part of the embodiments of the present invention, but not all embodiments. All other embodiments obtained by a person of ordinary skill in the art without any inventive step in connection with the embodiments of the present invention shall fall within the scope of protection of the present invention.

In the description of the present invention, it is to be understood that the terms "first", "second" and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.

With reference to fig. 1, the embodiment of the present invention provides a method for preparing an extract for treating gastric cancer, comprising the following steps:

s10, obtaining vinegar and traditional Chinese medicine components, wherein the traditional Chinese medicine components comprise: periplaneta americana, dandelion, pseudo-ginseng and ginseng;

s20, pretreating the traditional Chinese medicine components, crushing, and sieving by using a 10-80-mesh sieve to obtain powdery traditional Chinese medicine components;

s30, extracting the powdery traditional Chinese medicine components by using table vinegar to obtain an extracting solution;

s40, sequentially concentrating, filtering and sterilizing the extracting solution to obtain the extract for treating the gastric cancer.

According to the preparation method of the extract for treating the gastric cancer, provided by the embodiment of the invention, vinegar and traditional Chinese medicinal materials such as periplaneta americana, dandelion, pseudo-ginseng, ginseng and the like are taken as main raw materials, the traditional Chinese medicinal materials are crushed, the vinegar is taken as an extracting agent to extract the traditional Chinese medicinal materials, and then the extract is concentrated and sterilized to obtain the extract for treating the gastric cancer. The vinegar is used for extracting active substances in the Chinese medicinal material components, so that the effective active substances in the Chinese medicinal materials can be efficiently extracted and dissolved out, and the content of the active substances in the medicine is improved.

Specifically, in the step S10, vinegar and Chinese herbal medicine components are obtained, wherein the Chinese herbal medicine components include: periplaneta americana, dandelion, pseudo-ginseng and ginseng. The embodiment of the invention takes vinegar and traditional Chinese medicinal materials such as periplaneta americana, dandelion, pseudo-ginseng, ginseng and the like as main raw materials, and the vinegar and the traditional Chinese medicinal materials contain abundant active substances, so that the vinegar has a positive effect on treating gastric cancer.

The vinegar is edible vinegar, and as a traditional sour flavoring agent, the vinegar contains abundant nutritional ingredients such as polypeptide, polysaccharide, flavone, polyphenol, antioxidant enzyme, polyunsaturated fatty acid, lactic acid, gluconic acid, amino acid, trace elements, B vitamins and the like besides a large amount of acetic acid. Wherein the polysaccharide in the edible vinegar has effects of reducing blood sugar, reducing blood lipid, resisting oxidation, improving immunity, resisting virus and tumor, etc. The flavonoids have strong biological activity, have the functions of reducing blood fat and blood sugar, resisting inflammation and allergy, resisting bacteria and virus, can remove free radicals, resist oxidation and carcinogenic factors, inhibit blood flow of tumor tissues to supply required nutrition, improve immunity and the like, thereby achieving the effects of inhibiting and preventing cancers. The polyphenol has strong oxidation resistance, has good prevention effect on diseases caused by free radicals, and has good effects in resisting mutagenesis, resisting virus, inhibiting bacteria and diminishing inflammation and the like. Based on the abundant material basis in the vinegar, the medicine for the embodiment of the invention plays the multi-mechanism multi-target-point pharmacodynamic action in treating gastric cancer by the combined action of various active substances such as acetic acid, polypeptide, polysaccharide, flavone, polyphenol and the like and the cooperation of the components of the traditional Chinese medicinal materials.

Wherein the periplaneta americana in the traditional Chinese medicine components has the effects of removing blood stasis and detoxifying, resisting bacteria and reducing swelling, protecting liver and promoting diuresis, enhancing immunity, resisting tumors and the like; dandelion is sweet in nature and taste, slightly bitter and cold. Entering liver and stomach meridians, and has diuretic, laxative, jaundice treating, and gallbladder promoting effects; notoginseng radix, entering liver and stomach channels, has effects of removing blood stasis, stopping bleeding, subsiding swelling, relieving pain, enhancing immunity, promoting blood cell metabolism, and balancing and regulating blood cells; ginseng, entering spleen and lung meridians, has the functions of invigorating primordial qi, tonifying spleen and lung, promoting the production of body fluid, tranquilizing, resisting fatigue, resisting stress and enhancing immunity. The traditional Chinese medicinal materials such as the periplaneta americana, the dandelion, the pseudo-ginseng, the ginseng and the like are rich in various active substances with treatment effects on the gastric cancer, and the vinegar and the traditional Chinese medicinal materials such as the periplaneta americana, the dandelion, the pseudo-ginseng, the ginseng and the like have synergistic effects, so that the active substances of the gastric cancer treatment drug are further enriched, and the active substances in the raw materials can be better played, so that the gastric cancer treatment drug prepared by the embodiment of the invention has the effects of strengthening body resistance and banking up root, activating blood and dissolving stasis, and resisting cancer, detoxifying and treating tumors, has a remarkable treatment effect on the gastric cancer, relieves pains of patients, relieves economic pressure, and has few toxic and side.

As a preferred embodiment, the vinegar is selected from: one or more of Zhangjiajiuquxiangzhi vinegar, Shanxi mature vinegar, Zhenjiang aromatic vinegar, Fujia Yongchun mature vinegar, Lanzhonggning vinegar, Henan mature turtle special vinegar, Tianjin uniflow mature vinegar, Liyunggang dripping vinegar and Beijing Longmen rice vinegar. More preferably, the vinegar is Zhang Jiajiu Jiuquxiangzhi vinegar. The total flavone in the Zhang Jiajiu Jiuqu aromatic ester vinegar reaches 1800mg/100mL, and the content of lactic acid accounts for not less than 25% of the total acid; the soluble salt-free solid is not less than 25g/100mL and is far higher than the national standard 1.0g/100 mL; the content of the characteristic active peptide is not less than 4.5g/100 mL. In addition, the aspongopus japonicus ester vinegar also contains active substances such as polysaccharide, amino acid, ferulic acid, gamma-aminobutyric acid, ligustrazine, catechin, epicatechin and the like, and the active substances are high in abundance and high in medicinal value. The aspongopus fragrant ester vinegar contains rich active substances, has effects of sterilizing, removing toxic substances, preventing common cold, removing blood stasis, relieving swelling, astringing blood, stopping bleeding, expelling ascaris and killing trichomonad, and has remarkable therapeutic effect on digestive system diseases, especially gastric cancer.

In a preferred embodiment, the vinegar has a total acid concentration of 8 to 16g/100 ml. When the total acid concentration of the vinegar is 8-16g/100ml, the extraction efficiency of active substances in the traditional Chinese medicinal materials is optimal, and the active substances in the traditional Chinese medicinal materials can be extracted fully and efficiently. In some embodiments, the vinegar is prepared by solid state fermentation.

As a preferred embodiment, the preparation of the vinegar at least comprises: soaking the raw materials in water, steaming, and stewing to obtain raw materials to be fermented; and then, sequentially carrying out alcoholic fermentation, acetic fermentation, fumigating, vinegar pouring, vinegar freezing and aging on the raw materials to be fermented. Specifically, the preparation method of the vinegar comprises the following steps: a) adding water into the raw materials for soaking to ensure that the raw materials fully absorb water to obtain soaked grain raw materials; b) adding water accounting for 140-240% of the weight of the grain raw materials into the soaked grain raw materials, and stewing the materials for 15-80 min to obtain the raw materials to be fermented; c) adding famous wine fungus series yeast which accounts for 20-70% of the weight of the cereal raw material into the raw material to be fermented, uniformly mixing, adding water which accounts for 40-70% of the total weight of the cereal raw material, and fermenting for 15-730 days at 28-35 ℃ to obtain wine mash; d) adding grain processing peel substances accounting for 100-300% of the weight of the grain raw materials into the fermented glutinous rice as loose materials, uniformly mixing, adding vinegar fermented grains accounting for 5-15% of the weight of the grain raw materials and fermenting for 6-8 days for acetic fermentation for 8-360 days to obtain vinegar fermented grains; e) smoking 25-75% of the fermented vinegar residue obtained in the step d) at 60-80 ℃ for 4-8 days to obtain smoked fermented residue products; f) adding cold water and the light vinegar liquid for pouring vinegar into the rest fermented vinegar grains to increase the total weight to 2-3 times of the original weight, soaking for more than 12 hours, and pouring out the vinegar liquid to obtain a first vinegar liquid; heating the first vinegar liquid to 80-90 ℃, adding the smoked grain product, soaking for more than 10 hours, and leaching out the vinegar liquid again to obtain a second vinegar liquid; g) freezing the second vinegar liquid obtained in the step f) in a freezing environment to form vinegar blocks; then, placing the vinegar blocks in an environment with the temperature of 0-20 ℃ to slowly melt the vinegar blocks, and collecting flowing thick vinegar liquid; h) putting the concentrated vinegar liquid collected in the step g) into a brewing jar, and brewing in the open air for at least 12 months to obtain the edible vinegar. The contents of active substances such as flavone, polysaccharide, polyphenol, active peptide and the like in the vinegar prepared by the embodiment of the invention are high, so that on one hand, the vinegar is beneficial to exerting a better treatment effect; on the other hand, the method has better extraction effect on the traditional Chinese medicinal materials matched with the vinegar. Therefore, the content of each active substance in the prepared medicine for treating gastric cancer is higher, the synergistic coordination mechanism among the active substances is more complete, and the curative effect of the medicine is better.

Specifically, in the step S20, the Chinese medicinal material components are pretreated, crushed, and sieved with a 10-80 mesh sieve to obtain powdery Chinese medicinal material components. The pretreated Chinese medicinal material components are crushed, which is favorable for the extraction of active substances in the subsequent Chinese medicinal material components. On one hand, if the crushing granularity is too large, the extraction of active substances is slow, insufficient extraction is easy to occur, raw materials are wasted, and the process cost is increased. On the other hand, the small crushing particle size destroys the crystal phase structure of active substances in the traditional Chinese medicinal materials matched with vinegar, damages the efficacy of the active substances and increases the separation difficulty.

As a preferred embodiment, the method of the pretreatment is: cleaning the traditional Chinese medicine components, and drying for 68-80 hours at 45-65 ℃. The method comprises the steps of cleaning the Chinese medicinal material components with purified water, removing impurities such as silt and deteriorated medicinal materials in the Chinese medicinal material components, drying the cleaned Chinese medicinal material components to obtain cleaned dry Chinese medicinal material components, and carrying out follow-up pharmacy. The pretreated traditional Chinese medicine components can not introduce unnecessary impurity components to the subsequent pharmacy process, and the pollution of the medicines at the source is avoided.

In a preferred embodiment, the pulverization is performed by a pulverizer or a pulverizer. In some embodiments, the traditional Chinese medicine components are crushed by a high-speed crusher to enable the particle size of the traditional Chinese medicine components to reach 10-80 meshes.

Specifically, in step S30, vinegar is used to extract the powdered Chinese medicinal components to obtain an extract. In a preferred embodiment, the powdered Chinese medicinal materials are extracted 1 to 3 times with vinegar of 7 to 10 times volume, respectively, and the extracts are combined. The vinegar is used as an extraction solvent to extract powdery traditional Chinese medicinal material components for multiple times, on one hand, the vinegar greatly improves the dissolution of active substances of the traditional Chinese medicinal material components such as periplaneta americana, dandelion, pseudo-ginseng, ginseng and the like, and the active substances in the traditional Chinese medicinal material components are completely dissolved out through multiple extraction, so that the utilization rate of the traditional Chinese medicinal material components is improved, and the content of the active substances in the medicine is improved. On the other hand, solved the many-sided problem that exists of traditional soaking with white spirit, if: the dissolution efficiency of the soaking active substances is low, the dissolution speed is slow, and many patients can not drink alcohol and even have alcohol allergy. Through multiple extraction, active substances in medicinal materials matched with vinegar are fully dissolved out, the medicine is utilized at the maximum efficiency, and the content of the active substances in the medicine is increased, so that the curative effect of the medicine is improved. In some embodiments, a first extraction of powdered herbal ingredients is performed with 7 volumes of vinegar; then, vinegar with 8 times of volume is adopted to carry out secondary extraction on the Chinese medicinal material components after the primary extraction; and extracting the Chinese medicinal material components after the second extraction for the third time by using vinegar with 9 times of volume, and finally combining the extracted products of the three times to obtain the extracting solution.

As a preferred embodiment, the method of extracting includes: extracting for 1-5 hours at the temperature of 20-80 ℃ and the rotating speed of 50-80 r/min, and filtering to obtain filtrate, namely the extracting solution. According to the embodiment of the invention, low-temperature extraction is adopted, so that the damage of high-temperature decoction to effective components is effectively avoided, the active substances in the extract for treating gastric cancer are kept in the optimal active states, and the optimal drug properties of the active substances are favorably exerted.

Specifically, in step S40, the extract is subjected to concentration, filtration, and sterilization in this order to obtain an extract for treating gastric cancer. The excessive water in the extracting solution is removed through concentration treatment, and the effective active substances in the medicine are concentrated, thereby being beneficial to taking, storing and transporting the extracting solution. And then filtering to remove impurity particulate matters in the concentrated medicine. Through sterilization treatment, impurity bacteria and other substances which are inevitably introduced in the preparation process of the extracting solution are removed, and the extracting solution is purified, so that better drug effect is brought into play.

As a preferred embodiment, the method of the concentration process includes: freezing the extracting solution into solid blocks at the temperature of-25 to-18 ℃; and (3) placing the solid block of the extracting solution at room temperature, slowly melting the solid block, collecting the molten liquid, and stopping collecting when the solid block becomes white. According to the embodiment of the invention, freezing concentration treatment is adopted, water molecules in the extracting solution are solidified into ice crystals by utilizing the solid-liquid phase equilibrium relation of the extracting solution below a freezing point, and ice is removed by a mechanical means, so that the solvent water in the solution is reduced, the concentration of the solution is improved, and the solution is concentrated. The concentration method does not affect the activity of active substances in the extract. When the freezing temperature is too high, the extract is not fully frozen, the melting speed of the extract block is not balanced, the concentration effect is not good, and the water content in the concentrated solution is high. When the freezing temperature is too low, the energy consumption is correspondingly increased, and the concept of green environmental protection is not met. The concentration method adopted by the embodiment of the invention can effectively avoid the adverse chemical change and biochemical change among the components in the medicament, and has small nutrient loss of active substances.

As a preferred embodiment, the method of sterilization treatment includes: sterilizing for 30-60 minutes at the temperature of 100-110 ℃. According to the embodiment of the invention, a high-temperature sterilization mode is adopted, microorganisms such as bacteria in the extract are removed, and the stability and the use safety of the medicine are improved. When the sterilization temperature is too low, microorganisms such as bacteria in the concentrated solution cannot be fully killed; when the sterilization temperature is too high, the optimal activity of each active substance in the concentrated solution is not favorably maintained, and the high temperature has the risk of damaging the activity of the active substances in the concentrated solution, thereby influencing the curative effect of the medicine. The embodiment of the present invention does not specifically limit the filtration treatment, as long as the purpose of removing impurities in the concentrated solution can be achieved. In some embodiments, the filtering treatment is performed by using a titanium rod.

In some embodiments, the method of pre-processing comprises: cleaning the traditional Chinese medicine components, and drying for 68-80 hours at 45-65 ℃; the crushing method comprises the following steps: crushing by a high-speed crusher or a pulverizer; the filtering treatment method comprises the following steps: filtering with titanium rod; the method for sterilizing treatment comprises the following steps: sterilizing for 30-60 minutes at the temperature of 100-110 ℃.

In some embodiments, the method of extracting comprises: the extraction method comprises the following steps: extracting for 1-5 hours at the temperature of 20-80 ℃ and the rotating speed of 50-80 r/min, and filtering to obtain filtrate, namely extracting solution; the concentration treatment method comprises the following steps: freezing the extracting solution into solid blocks at the temperature of-25 to-18 ℃; and (3) placing the solid block of the extracting solution at room temperature, slowly melting the solid block, collecting the molten liquid, and stopping collecting when the solid block becomes white.

As a preferred embodiment, the method for preparing the extract for treating gastric cancer further comprises the steps of: adding auxiliary materials allowed in the medical scope into the extracting solution after the concentration and sterilization treatment, and preparing into pharmaceutically acceptable dosage forms. The auxiliary materials can be a stabilizer, a thickening agent, a dispersing agent, a preservative, a disintegrating agent, a sustained-release agent, an excipient or a combination thereof, mainly play roles in stabilizing, emulsifying, bonding, disintegrating, filling and the like on the medicine for treating the gastric cancer, are beneficial to improving the stability of the medicine, meet the requirements of different preparations and improve the use feeling of the medicine.

As a preferred embodiment, the adjuvant is selected from: one or more of glycerin, polysorbate, polyethylene glycol, high-acyl gellan gum, xanthan gum, carrageenan, sodium alginate, sodium carboxymethylcellulose, microcrystalline cellulose, hydroxypropyl methylcellulose, crospovidone, polyacrylic resin polymers, safflower seed oil, lecithin and beeswax, and the specific auxiliary materials can be selected and added according to factors such as dosage form, taste and the like of the medicine.

As a preferable example, the dosage form of the drug for treating gastric cancer according to the embodiment of the present invention is not particularly limited, and may be prepared into dosage forms according to specific use conditions. Preferably, the medicament for treating prostate diseases is prepared into a pharmaceutically acceptable dosage form, which comprises: at least one dosage form of oral liquid, capsule, tablet, paste, powder, granule, pill, suppository and extract injection, and the specific preparation type is determined according to actual requirement and condition. The medicine for treating gastric cancer provided by the embodiment of the invention can be prepared into various dosage forms, is flexible and convenient in preparation, has no special limitation, can meet the requirements of different dosage forms, and is convenient to prepare and wide in applicability.

Correspondingly, the embodiment of the invention provides an extract for treating gastric cancer, which comprises the following raw materials in parts by weight: 300-800 parts of table vinegar, 8-20 parts of periplaneta americana, 10-20 parts of dandelion, 4-16 parts of pseudo-ginseng, 5-20 parts of ginseng and 0-30 parts of auxiliary materials. The raw material proportion in parts by weight is beneficial to the components to exert the optimal drug curative effect, and the 300-800 parts of vinegar can fully extract active substances in medicinal materials such as 10-20 parts of dandelion, 4-16 parts of pseudo-ginseng, 5-20 parts of ginseng and the like, and simultaneously, the active substances can exert the optimal synergistic cooperation effect to improve the drug curative effect.

According to the extract for treating gastric cancer, provided by the embodiment of the invention, vinegar is adopted as a medicinal raw material and is matched with traditional Chinese medicine materials such as periplaneta americana, dandelion, pseudo-ginseng, ginseng and the like, and on one hand, the components such as flavone, active peptide, polysaccharide, polyphenol and the like in the vinegar can exert the effects of various active substances; on the other hand, the vinegar can improve the dissolution of the active substances of the ingredients of the traditional Chinese medicinal materials such as the periplaneta americana, the dandelion, the pseudo-ginseng, the ginseng and the like, improve the content of the active substances in the medicament, realize multi-mechanism and multi-target performance of the medicament property through the synergistic cooperation effect of the vinegar and the active substances in the traditional Chinese medicinal materials, achieve the aims of improving the microenvironment of tumor growth, regulating the tumor immune metabolism and inhibiting the proliferation and the metastasis of tumor cells, and further regulate and control the occurrence and the development of tumors. The medicine for treating gastric cancer provided by the invention has obvious analgesic, sedative and anticancer effects, reduces the toxic and side effects of the medicine while improving the medicine effect of the medicine, improves the safety of the medicine, and has excellent performance in the aspects of enhancing the chemotherapy effect, improving the immunity of the organism, inhibiting the growth of cancer cells, improving symptoms and the like, so that the purposes of improving the life quality of a patient and prolonging the life time are achieved, the cost is low, the economic pressure of the patient can be effectively relieved, and the medicine is easy to accept by the patient and is a safe and effective treatment medicine. In some embodiments, the extract for treating gastric cancer is prepared by the preparation method of the extract for treating gastric cancer.

In some embodiments, the extract for treating gastric cancer has a polypeptide concentration of 18-22 mg/ml, a polysaccharide concentration of 12-14 mg/ml, a flavone concentration of 22-28 mg/ml and a ginsenoside concentration of 3-6 mg/ml. The extract for treating gastric cancer provided by the embodiment of the invention is rich in a combination of various active substances such as vinegar polypeptide, vinegar polyphenol, periplaneta americana polypeptide, dandelion polysaccharide, ginsenoside, flavone and the like, and has a remarkable effect on digestive system diseases, particularly gastric cancer, through the synergistic effect of the active substances. On one hand, the traditional Chinese medicine composition can effectively relieve and cure adverse symptoms of emaciation, pain and the like of a patient with the gastric cancer disease, and has the effects of relieving symptoms, improving the life quality of the patient and delaying the life. On the other hand, the extract for treating gastric cancer provided by the embodiment of the invention takes vinegar, periplaneta americana and the like as main raw materials, so that the extract has the advantages of wide raw material source, easiness in obtaining, low medicine cost and capability of relieving the economic pressure of patients.

In order to make the above implementation details and operations of the present invention clearly understood by those skilled in the art and to make the progress of the extract for treating gastric cancer and the preparation method thereof obvious in the present invention, the above technical solution is illustrated by the following examples.

Example 1

An oral liquid for treating gastric cancer.

Weighing 15kg of periplaneta americana, 10kg of dandelion, 4kg of pseudo-ginseng and 5kg of ginseng;

removing sand and other impurities in Periplaneta americana, herba Taraxaci, Notoginseng radix, and Ginseng radix, cleaning with purified water, respectively, and oven drying at 55 deg.C for 72 hr; mixing the medicines according to the formula, crushing by a high-speed crusher, and sieving by a 10-mesh sieve;

weighing 160L of aspongopus ester vinegar, adding into an extraction tank, stirring, heating to 50 deg.C, adding pulverized periplaneta americana, herba Taraxaci, Notoginseng radix, and Ginseng radix, extracting for 4h, and filtering to obtain a first extractive solution; weighing 140L of aspongopus aromatic ester vinegar, adding into an extraction tank, stirring, heating to 50 deg.C, adding above residue, extracting for 4 hr, and filtering to obtain a second extractive solution; combining the first and second extracts;

cooling the extract to room temperature, subpackaging the extract in a specially-made stainless steel small barrel, placing the stainless steel small barrel in a refrigeration house at the temperature of-18 ℃ for freezing, forming ice blocks, taking out the ice blocks, placing the ice blocks in the room temperature, slowly cooling, melting, collecting melted liquid, stopping collecting when the ice blocks become white, obtaining concentrated solution with the total acid of 10g/100ml, performing titanium rod precision filtration, sterilization, filling into 10 ml/piece, performing sterilization according to the standard operation procedure of an oral liquid sterilizer, wherein the sterilization temperature is 105 ℃, the sterilization time is 30min, and after heating, sterilization, leak detection, cooling and cleaning, obtaining an oral liquid product 1, wherein the polypeptide concentration is 18.2mg/ml, the polysaccharide concentration is 12.6mg/ml, the flavone concentration is 24.6mg/ml, and the ginsenoside concentration is 4.1 mg/ml.

Example 2

An oral liquid for treating gastric cancer.

Weighing 18kg of periplaneta americana, 15kg of dandelion, 6kg of pseudo-ginseng and 7.5kg of ginseng;

weighing 288L of aspongopus ester vinegar, adding into an extraction tank, stirring, heating to 45 deg.C, adding pulverized periplaneta americana, herba Taraxaci, Notoginseng radix, and Ginseng radix, extracting for 4 hr, and filtering to obtain a first extractive solution; weighing 216L of aspongopus japonicus ester vinegar, adding into an extraction tank, stirring, heating to 45 deg.C, adding above filter residue, extracting for 4 hr, and filtering to obtain a second extractive solution; combining the first and second extracts;

cooling the extract to room temperature, subpackaging the extract in a specially-made stainless steel small barrel, placing the stainless steel small barrel in a refrigeration house at the temperature of-18 ℃ for freezing, forming ice blocks, taking out the ice blocks, placing the ice blocks in the room temperature, slowly cooling, melting, collecting melted liquid, stopping collecting when the ice blocks become white, obtaining concentrated solution with the total acid of 10g/100ml, performing titanium rod precision filtration, sterilization, filling into 10 ml/piece, performing sterilization according to the standard operation procedure of an oral liquid sterilizer, wherein the sterilization temperature is 105 ℃, the sterilization time is 30min, and after heating, sterilization, leak detection, cooling and cleaning, obtaining an oral liquid product 2, wherein the polypeptide concentration is 19.3mg/ml, the polysaccharide concentration is 12.1mg/ml, the flavone concentration is 25.9mg/ml, and the ginsenoside concentration is 4.8 mg/ml.

Example 3

An oral liquid for treating gastric cancer.

Weighing 22.5kg of periplaneta americana, 18kg of dandelion, 7.2kg of pseudo-ginseng and 9kg of ginseng;

weighing and adding 310L of aspongopus japonicus ester vinegar into an extraction tank, stirring and heating to 55 ℃, adding the crushed ingredients of the traditional Chinese medicinal materials of the periplaneta americana, the dandelion, the pseudo-ginseng and the ginseng, extracting for 4 hours, and filtering to obtain a first extracting solution; weighing 240L of aspongopus japonicus ester vinegar, adding into an extraction tank, stirring, heating to 50 deg.C, adding above filter residue, extracting for 4 hr, and filtering to obtain a second extractive solution; combining the first and second extracts;

cooling the extract to room temperature, subpackaging the extract in a specially-made stainless steel small barrel, placing the stainless steel small barrel in a refrigeration house at the temperature of-18 ℃ for freezing, forming ice blocks, taking out the ice blocks, placing the ice blocks in the room temperature, slowly cooling, melting, collecting melted liquid, stopping collecting when the ice blocks become white, obtaining concentrated solution with the total acid of 10g/100ml, performing titanium rod precision filtration, sterilization, filling into 10 ml/piece, performing sterilization according to the standard operation procedure of an oral liquid sterilizer, wherein the sterilization temperature is 105 ℃, the sterilization time is 30min, and after heating, sterilization, leak detection, cooling and cleaning, obtaining an oral liquid product 3, wherein the polypeptide concentration is 15.2mg/ml, the polysaccharide concentration is 12.1mg/ml, the flavone concentration is 14.6mg/ml, and the ginsenoside concentration is 2.1 mg/ml.

Furthermore, in order to verify the efficacy of the drug prepared in the present invention in treating gastric cancer, the present invention has performed human drug efficacy experiments.

Test example 1

Li Yi, male, 63 years old, Hunan Changde, 58 years old, found the cancer embryo abnormal in a physical examination, in Zhongnan university Hunan ya Hospital further examination confirmed gastric cancer early stage, adopting operation treatment, three years later, chest pain, vomiting, hematochezia, through the examination confirmed gastric cancer recurrence and spread to the lung, two courses of chemotherapy, patients can not bear the pain caused by chemotherapy, and the symptoms do not obviously improve, do not want to continue chemotherapy. After the oral liquid 1 for treating the gastric cancer, which is prepared by the embodiment of the invention, is taken for 26 days, the hematochezia and the pain are reduced, the diet is normal, the oral liquid is continuously taken for 6 months, the hematochezia and the pain are not obvious, the cancer focus is reduced through the examination of a hospital, and the normal life with the cancer can be realized.

Test example 2

The oral liquid 2 prepared by the embodiment of the invention is taken three times a day, one at a time, for 30 days continuously, the appetite is obviously improved, the pain is relieved, the vomiting phenomenon is not obvious, the CT detection focus does not obviously change, the patient is unwilling to continue the chemotherapy treatment, the oral liquid 2 can be taken for 180 days, the oral liquid can be swallowed normally, and the pain is not obvious, no vomit phenomenon, the focus is reduced through hospital detection, and the normal life of the patient is not affected.

Test example 3

Certain old man, 55 years old man, Hunan Changsha, abdominal pain, inappetence, vomiting, diagnosis, gastric cancer antigen detection, CT detection and ultrasonic endoscopy examination, confirms that the stomach cancer is in the middle and late stages, has no obvious effect after 2 courses of chemotherapy, is weak, cannot be continuously treated by the chemotherapy, has intolerable pain, and can only relieve the pain by means of the hibitalin, the morphine and the dolantin. After the oral liquid 3 prepared by the embodiment of the invention is taken for 30 days, the frequency of using the analgesic is reduced, the food intake is increased, the oral liquid is taken for 3 times every day, 1 count each time, the injection taking and the analgesic taking are stopped after the oral liquid is taken for 3 months, and patients with advanced gastric cancer cannot be seen basically after the oral liquid is taken normally.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.

Claims

1. The preparation method of the extract for treating gastric cancer is characterized by comprising the following steps:

2. The method for preparing an extract for treating gastric cancer according to claim 1, wherein the vinegar is selected from the group consisting of: one or more of Zhangjiajiuquxiangzhi vinegar, Shanxi mature vinegar, Zhenjiang aromatic vinegar, Fujia Yongchun mature vinegar, Lanzhonggning vinegar, Henan mature turtle special vinegar, Tianjin uniflow mature vinegar, Liyunggang dripping vinegar and Beijing Longmen rice vinegar.

3. The method for preparing an extract for treating gastric cancer according to claim 1, wherein the vinegar has a total acid concentration of 8 to 16g/100 ml.

4. The method of preparing an extract for treating gastric cancer according to claim 1, wherein the vinegar is prepared by at least: soaking the raw materials in water, steaming, and stewing to obtain raw materials to be fermented; and then, sequentially carrying out alcoholic fermentation, acetic fermentation, fumigating, vinegar pouring, vinegar freezing and aging on the raw materials to be fermented.

5. The method for preparing an extract for the treatment of gastric cancer according to any one of claims 1 to 4, wherein the step of pretreating comprises: cleaning the traditional Chinese medicine components, and drying for 68-80 hours at 45-65 ℃; and/or the presence of a gas in the gas,

6. The method for preparing an extract for treating gastric cancer according to any one of claims 1 to 4, wherein the extraction step comprises: extracting for 1-3 times under the conditions that the temperature is 20-80 ℃, the rotating speed is 50-80 r/min and the extracting time is 1-5 hours; and/or the presence of a gas in the gas,

the concentration treatment step comprises: freezing the extracting solution into solid blocks at the temperature of-25 to-18 ℃; and (3) placing the solid block of the extracting solution at room temperature, slowly melting the solid block, collecting the molten liquid, and stopping collecting when the solid block becomes white.

7. The method for preparing an extract for treating gastric cancer according to any one of claims 1 to 4, wherein the method for preparing the extract for treating gastric cancer further comprises: adding auxiliary materials allowed in the medical scope into the extracting solution after the concentration and sterilization treatment, and preparing into pharmaceutically acceptable dosage forms.

8. The method for preparing an extract for treating gastric cancer according to claim 7, wherein the adjuvant is selected from the group consisting of: at least one of glycerin, polysorbate, polyethylene glycol, high acyl gellan gum, xanthan gum, carrageenan, sodium alginate, sodium carboxymethylcellulose, microcrystalline cellulose, hydroxypropyl methylcellulose, crospovidone, polyacrylic resin polymers, safflower seed oil, lecithin and beeswax; and/or the presence of a gas in the gas,

9. The extract for treating the gastric cancer is characterized by comprising the following raw materials in parts by weight: 800 parts of vinegar, 8-20 parts of periplaneta americana, 10-20 parts of dandelion, 4-16 parts of pseudo-ginseng and 5-20 parts of ginseng.

10. The extract for treating gastric cancer according to claim 9, wherein the concentration of the polypeptide is 18-22 mg/ml, the concentration of the polysaccharide is 12-14 mg/ml, the concentration of the flavone is 22-28 mg/ml, and the concentration of the ginsenoside is 3-6 mg/ml.